Spg012846 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012846
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionER membrane protein complex subunit 8/9 homolog
Locationscaffold1: 18684778 .. 18688748 (-)
RNA-Seq ExpressionSpg012846
SyntenySpg012846
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGTTCTCACTTCTCTGCTACCCGTCCATCCGGCTTCACTTTGGCTGTAAACTCCGCCTCCTCCACCTCGCTTTCACCGTTTTTTCCGGCGGACTCACCGGCGATTCACCAACTCGCTGACTCATGGGCGGCGATCTACGCTTCGAGATCTCTCAGAATGCCTACATAAAGCTCGTTCTCCATGCCCTAAAGCACCGGACCTCCGCCGTCAACGGAGTCCTCCTAGGTCGCAATTCCGGCCCCAATGATGTCGTCGAAATTGCCGATTCCGTCCCTCTCTTCCACTCCAGCATCGGTCTCCTCCCTCAGCTCGAGATATCTCTCCTTCTGGTACTCCTTTTGAACCATTTTGCAGCGAAATTTTTTGTTTAGATTTCTGTGAACTGGTGGCTCATGTGAGAATTTGTTGTGTTTGACGCCATGTTATATTGCCTTTTTTACTTGCGTATTTGAGATCTGTGATTGATGAGCATGCTTGATTTGGAATCAGATTGAAGAATATTATGAAGCTAAGGGATTGAGTATCGTGGGATATTTTCATGCTAACGAGCGGTTTGATGACTATGAGGTCGGCGGTGTTGCCAAGAATATTGGTGATCATATTTCTCGATCATTCCCTCATGCTGCTCTTCTTTTGGTAAGATATGTCTTACTGTACTTTAACTGTATTGTTTTGTATATTTCTGTTGTTTGATTTATGCTCCTCCGCAGTTAGATCACAGAAAACTTGAAGCTTTGCCGAAGAGAAAGGATAGAAGCCCTGTAATGGAGGTGAGTTTATGGCTATAGAATTCTTATCATGGTTTGTAAATTGTATTTGTGCGCTTCATTATAATTTCCACTAAACTTTCCTCCTTTTATTCTGTTTCAAAATCATTCAAACGAGCAATTTTATATCGCAAAAATGCAAAATTTAAATATTGTTGGTTGATGCTGTTATGGCATGTTGTTCTTTGGGACCAACTACTTTGTTCCGTTTTTGAAGGACAGCAAAACATCGGAAAAATAACTTTGTTACATCATTGATGATTCCTTCATTTATTGGGGTGACGTTCTTATAGTTTTCCCAATCACTAGATCGGACACAATGCTAAAATGTCACAAGAAGGCTTCATTTTAATTCTATTTGAGTCTTTTGACAATCTCTCGATTATCTTCCATTGGTGGAGACTTGATATCATTAACTACTACTGATGCTATTTGAGATATAACGAAATGTATGTCGAAGGTTCACTGGCTTTCTGTCTTGTTTATCTTAAATTCCAGCTTGTAACTAGACCTTTTTATTGAATTTTCTGAGCCTTTTTAATGTTATTTTAAATTTGGTTTAGCTTGTACTTATTTTGCATTGAAACGGATGAAGTATGTCGGGGTTTCTTTCAAGGAGGTGAGAGCTTGAGAGAGGCAATAGGAGGGCGATCTCCAATTTTCCTTAATGTTGGAGCTGCTCCATCTTTAGATGTAGGATTGAACATAAGATGATCAACATGGAATTTGAGAAAGGATATAGGAGAAACATCATTCGATCGATTTTTCCCAATTAAATGGTGAGTCATTGCACTCCTTTCTTTAGTTTGCCCCTCAACCACCAGAGTTTATGCATAACGTTCTATATCAAAGGAGAATCTTGAGACCTACATTGTAGAGTGGTTGCTTGAAGCTATGGGGGTTTTCTGTTGTAAATCCTTTTATGATTTTTTGAGGCAAGATCCAAGTGTTACACTGAATAAGATTGATTCAACTTGGAAGTTTAAGATCTCAAAGAAGTTTATTGTGCAAACAGTGATTGAGAGAGTGCAGTAAGGGAAGCAAATACACGCCCATCCATATTGTGAAGCAGTTATGGAACACCGGGATGTTCTCCTTTGTTCTTTTGCCTTTGCAAGAAGCATTTAGGAGAAGTTGTAGTGGCATTTGATCTTTGTATGAGGGCTCCTAGTGTTGATTATTATCTCGTTTACCATGGTTCAGGTTGAACCATCAGTTTGCTTTTGTCCTCTCCCTACGGTATATGTTTCCTTATTAGTACATCTTTGGCCTCAAAAGTGCGGAAGATCACTTGTTAACTTTTTTTAGTTGGAGGAGGTAACAGTTATGTGTTGTCTTACTGGTCAAAAGTATAAACTTTGAGTTCAACTCTGTTAGGCCCCCAACTACCATTCGTTATGAGAGGAGGGATAAGAAGTTAGTTAGAAGGTCAGGTGCATGGGAGTGAGGAACCACCTGAGGTTTGTTGAGATAGAATGTCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGTATGAGTATTTAGGTAAGAAGGGAGTAGTATTGTTTTCTTTTATGCTTTTGTCTATGAACAGGTTGTAGAGGGAGAGGAAAGATTACCTGCCCTCTCTAATTGGTTGGGAGTGTTTGTATTGCCTTAGGGCATTGTTCCTCCTTTGTTTATCAATATATCGTAAGGGAGGCTTACAAACTTCAGCCGCTGTAGTTTATAAGATCAATCTAGATGTGTTAGTGACTGGCAATTGTTTAACCCAGAAGATAAAAAGATCTCGACAAAGTCAAACTCATTACTAAAGACTAAAGTAAATGACAGTAAGCGTTGTCAGTGGAAATTTACTTATTCTGGGATTTTGAGTGTTTTTCTTTGCTAGGATAGATCCTGATTGCTGCTTGTTCCAATCGCTTTCCAATTTCATTTATCTCTTAAGCATCACTATTTGACTTATCTAATGTCAAACTTGTAGTCTAAAATGGTGAGTTGTCTTGTGACCTAGCAAAGAAATATATTGGGAGACTAAGTGGGGTGTGGCCGATTTTGAGCATTGTCTCAGCACACCCGACTGAGGCTCCCAATTCCCTTATTTATTATCAAGACCCCTAACTAATTACCAAGACACTCTTATATAATTACCACTATGCCCTTACTACTATTCTACTACTATTCCTAATATATCTCTATTAGTACTCTCACACCCCACACTGAGACCGTGCTCAAAATCGTCCACACTCCACTAAGGCTCCCAATTCCCCTATCTATAATCAAGAGTCAAGACCCCTAACTAATTACCAAGACACCCTTATCTAATCACCACTATGTCCTCACTACTGTTCTACTACTATTCCCAATATCTCTGTCTCTGTTACTACTCTCACACCGGACTAGCAGTCATGTAATCTAGGAGGAACCCTTTTAAACTATAGGCCTTGTCTATGTTGTTAGTTTCACCATGAAATAAAGTAGACCCTGAGCATGAGGAGATGATTGCATAATGTGCTTCTCATTCCAATTTTTTTTTGTTATGTTGCCGATCAGCTATACACTAAGGAAACTTCCAAGCATTGGAAGCTTGCTGGATCAGATGGAAGCAGCCAGCTAACGATCAAGGAACCGTCAGCAAATGTGGTCCTGTTGGATTTCATATCATCTGAAAAATGGCAAGACGTGGTGGATTTTGATGATCACCTCGACGACATTAGCAAGTACGATACACAATGCCTCCTTTTTTTGAACATTGCATCAATCATGAATATAGTTATACCTTATTCTTTTAAACTTTGAAAACTTTCAGGGATTGGTTGAACCCAGACCTCTTCAAGTAGATCTTTTCCCATAGTTGCAAGGCTTGATTGCTCTAGAAAGATAAGTCGGAAACTGGAGCCTTGTGCTTTTCAAGGATTGAGAATATTGTTAGATGTATTGCTATTTAGTCATCGTCGAATTTTATCATGTTAGACTTTGATTACTCGTCTTTGATTCGAGATACTGAATAGAAGTTCAGTCTTGAAATTGAATTTGAAGCCCTATTATTTTAGCATTTCAAGTTTGGGGCAAATATTTAAGGAGTTGTTTGGATGTACACTAACATAATATGTAATTCTTTGGTGAGAAGACAAATACTCTTTAGCATTGGGTTTTAGTTTATTGGTTGAGAATAAGTTTGAATGAGAATTTTGGGAATGAAATGATGAGGATGAGAATAG

mRNA sequence

ATGGGCGGCGATCTACGCTTCGAGATCTCTCAGAATGCCTACATAAAGCTCGTTCTCCATGCCCTAAAGCACCGGACCTCCGCCGTCAACGGAGTCCTCCTAGGTCGCAATTCCGGCCCCAATGATGTCGTCGAAATTGCCGATTCCGTCCCTCTCTTCCACTCCAGCATCGGTCTCCTCCCTCAGCTCGAGATATCTCTCCTTCTGATTGAAGAATATTATGAAGCTAAGGGATTGAGTATCGTGGGATATTTTCATGCTAACGAGCGGTTTGATGACTATGAGGTCGGCGGTGTTGCCAAGAATATTGGTGATCATATTTCTCGATCATTCCCTCATGCTGCTCTTCTTTTGTTAGATCACAGAAAACTTGAAGCTTTGCCGAAGAGAAAGGATAGAAGCCCTGTAATGGAGCTATACACTAAGGAAACTTCCAAGCATTGGAAGCTTGCTGGATCAGATGGAAGCAGCCAGCTAACGATCAAGGAACCGTCAGCAAATGTGGTCCTGTTGGATTTCATATCATCTGAAAAATGGCAAGACGTGGTGGATTTTGATGATCACCTCGACGACATTAGCAAGGATTGGTTGAACCCAGACCTCTTCAAGTAG

Coding sequence (CDS)

ATGGGCGGCGATCTACGCTTCGAGATCTCTCAGAATGCCTACATAAAGCTCGTTCTCCATGCCCTAAAGCACCGGACCTCCGCCGTCAACGGAGTCCTCCTAGGTCGCAATTCCGGCCCCAATGATGTCGTCGAAATTGCCGATTCCGTCCCTCTCTTCCACTCCAGCATCGGTCTCCTCCCTCAGCTCGAGATATCTCTCCTTCTGATTGAAGAATATTATGAAGCTAAGGGATTGAGTATCGTGGGATATTTTCATGCTAACGAGCGGTTTGATGACTATGAGGTCGGCGGTGTTGCCAAGAATATTGGTGATCATATTTCTCGATCATTCCCTCATGCTGCTCTTCTTTTGTTAGATCACAGAAAACTTGAAGCTTTGCCGAAGAGAAAGGATAGAAGCCCTGTAATGGAGCTATACACTAAGGAAACTTCCAAGCATTGGAAGCTTGCTGGATCAGATGGAAGCAGCCAGCTAACGATCAAGGAACCGTCAGCAAATGTGGTCCTGTTGGATTTCATATCATCTGAAAAATGGCAAGACGTGGTGGATTTTGATGATCACCTCGACGACATTAGCAAGGATTGGTTGAACCCAGACCTCTTCAAGTAG

Protein sequence

MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLLPQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDHRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQDVVDFDDHLDDISKDWLNPDLFK
Homology
BLAST of Spg012846 vs. NCBI nr
Match: XP_008455761.1 (PREDICTED: ER membrane protein complex subunit 8/9 homolog [Cucumis melo] >KAA0025782.1 ER membrane protein complex subunit 8/9-like protein [Cucumis melo var. makuwa] >TYK09673.1 ER membrane protein complex subunit 8/9-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 390.6 bits (1002), Expect = 8.4e-105
Identity = 194/203 (95.57%), Postives = 199/203 (98.03%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHR+SAVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRSSAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPVMELYTKETSK+WKLAGSDGSSQL IKEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. NCBI nr
Match: XP_038880935.1 (ER membrane protein complex subunit 8/9 homolog [Benincasa hispida])

HSP 1 Score: 390.6 bits (1002), Expect = 8.4e-105
Identity = 194/203 (95.57%), Postives = 198/203 (97.54%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MG DLRFEISQNAYIKLVLHALKHRTSAVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGSDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLE LPKRKDRSPVMELYTKETSK+WKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEVLPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. NCBI nr
Match: XP_004144311.1 (ER membrane protein complex subunit 8/9 homolog [Cucumis sativus] >KGN54668.1 hypothetical protein Csa_012212 [Cucumis sativus])

HSP 1 Score: 389.8 bits (1000), Expect = 1.4e-104
Identity = 193/203 (95.07%), Postives = 199/203 (98.03%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRT+AVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTTAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPVMELYTKETSK+WKLAGSDGSSQL +KEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMMKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. NCBI nr
Match: KAG6595444.1 (ER membrane protein complex subunit 8/9-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027444.1 ER membrane protein complex subunit 8/9-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 385.6 bits (989), Expect = 2.7e-103
Identity = 191/203 (94.09%), Postives = 198/203 (97.54%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGR SG NDV+EIA+SVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRISGSNDVIEIAESVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGLSIVGYFHANERFDDY+ GGVAKNI DHISRSFPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYDAGGVAKNIADHISRSFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPV+ELYT+ETSK+WKLAGSDGSSQLT+KEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVVELYTRETSKNWKLAGSDGSSQLTMKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. NCBI nr
Match: XP_023518172.1 (ER membrane protein complex subunit 8/9 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 384.4 bits (986), Expect = 6.0e-103
Identity = 191/203 (94.09%), Postives = 198/203 (97.54%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGR SG NDV+EIA+SVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRISGSNDVIEIAESVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGLSIVGYFHANERFDDY+VGGVAKNI DHISRSFPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYDVGGVAKNIADHISRSFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPV+ELYT+ETSK+WKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVVELYTRETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
            V+DFDDHLDDISKDWLNPDLFK
Sbjct: 181 GVMDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. ExPASy Swiss-Prot
Match: Q9FG71 (ER membrane protein complex subunit 8/9 homolog OS=Arabidopsis thaliana OX=3702 GN=EMB2731 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.1e-78
Identity = 143/201 (71.14%), Postives = 177/201 (88.06%), Query Frame = 0

Query: 3   GDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPND-VVEIADSVPLFHSSIGLLP 62
           G+L++EISQNAYIKLVLH+L+H+T+AVNGVL+GR S  +D VVEI+DSVPLFHS++ LLP
Sbjct: 7   GELKYEISQNAYIKLVLHSLRHKTAAVNGVLVGRISPKDDGVVEISDSVPLFHSNLALLP 66

Query: 63  QLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDH 122
            LEISL++IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIGDHISR FP A +LLL++
Sbjct: 67  PLEISLIMIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIGDHISRYFPQAPILLLNN 126

Query: 123 RKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQD 182
           +KLEAL K K+RSPVM+L  K+ SK+W++ G+DG S+L +KEPSANVVL D+ISSEKW+D
Sbjct: 127 KKLEALSKGKERSPVMQLCVKDASKNWRVVGADGGSKLLLKEPSANVVLSDYISSEKWKD 186

Query: 183 VVDFDDHLDDISKDWLNPDLF 203
           V D DDHLDD++KDWLNP LF
Sbjct: 187 VTDVDDHLDDVTKDWLNPGLF 207

BLAST of Spg012846 vs. ExPASy Swiss-Prot
Match: Q5FVL2 (ER membrane protein complex subunit 8 OS=Rattus norvegicus OX=10116 GN=Emc8 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.9e-23
Identity = 63/204 (30.88%), Postives = 102/204 (50.00%), Query Frame = 0

Query: 8   EISQNAYIKLVLHALKHRTSAVNGVLLGRNS--------GPNDVVEIADSVPLFHSSIGL 67
           +++  AY K+VLH  K+   AVNG+L+            G  +     D +PLFH ++ L
Sbjct: 5   KLTTQAYCKMVLHGAKYPHCAVNGLLVAERQRPRKEHPPGAGNHTLFVDCIPLFHGTLAL 64

Query: 68  LPQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLL 127
            P LE++L LI+ + +     I GY+ ANER  D     VA+ +   I+  F  AAL+++
Sbjct: 65  TPMLEVALTLIDSWCKDNSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFSDAALIMV 124

Query: 128 DHRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKW 187
           D+ K          +P + +Y +  ++ W+    D         P A  +    + S  +
Sbjct: 125 DNAKF----TMDCAAPTIHVYEQHENR-WRC--RDPHHDYCEDWPEAQRISASLLDSRSY 184

Query: 188 QDVVDFDDHLDDISKDWLNPDLFK 204
           + +VDFD+HLDDI  DW NP++ K
Sbjct: 185 ETLVDFDNHLDDIRSDWTNPEINK 201

BLAST of Spg012846 vs. ExPASy Swiss-Prot
Match: O70378 (ER membrane protein complex subunit 8 OS=Mus musculus OX=10090 GN=Emc8 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.9e-23
Identity = 63/204 (30.88%), Postives = 101/204 (49.51%), Query Frame = 0

Query: 8   EISQNAYIKLVLHALKHRTSAVNGVLLGRNS--------GPNDVVEIADSVPLFHSSIGL 67
           +++  AY K+VLH  K+   AVNG+L+            G        D +PLFH ++ L
Sbjct: 5   KLTTQAYCKMVLHGAKYPHCAVNGLLVAERQRPRKEHPPGAGSHTLFVDCIPLFHGTLAL 64

Query: 68  LPQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLL 127
            P LE++L LI+ + +     I GY+ ANER  D     VA+ +   I+  F  AAL+++
Sbjct: 65  TPMLEVALTLIDSWCKDNSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFGDAALIMV 124

Query: 128 DHRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKW 187
           D+ K          +P + +Y +  ++ W+    D         P A  +    + S  +
Sbjct: 125 DNAKF----TMDCAAPTIHVYEQHENR-WRC--RDPHHDYCEDWPEAQRISASLLDSRSY 184

Query: 188 QDVVDFDDHLDDISKDWLNPDLFK 204
           + +VDFD+HLDDI  DW NP++ K
Sbjct: 185 ETLVDFDNHLDDIRSDWTNPEINK 201

BLAST of Spg012846 vs. ExPASy Swiss-Prot
Match: Q9DB76 (ER membrane protein complex subunit 9 OS=Mus musculus OX=10090 GN=Emc9 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 6.4e-23
Identity = 68/194 (35.05%), Postives = 102/194 (52.58%), Query Frame = 0

Query: 8   EISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLLPQLEISL 67
           EIS  AY K+ LHA ++  +AVNG+LL   +G  + + + D VPLFHS + L   LE++L
Sbjct: 5   EISARAYGKMCLHASRYPHAAVNGLLLAPATGSGECLCLTDCVPLFHSHLALSVMLEVAL 64

Query: 68  LLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDHRKLEAL 127
             ++ +    GL + GY+HAN   DD   G +A  I   I+  FP A L++LD++KL   
Sbjct: 65  NQVDVWGAQAGLVVAGYYHANAVLDDQSPGPLALKIAGRIAEFFPRAVLIMLDNKKLVTR 124

Query: 128 PKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQDVVDFDD 187
           P+     PV+ L  +     W     D +  +      +  ++   +     Q +VDFD 
Sbjct: 125 PR---VPPVIVL--ENQGLQW--VPKDKNLVMWRDWEESRQMVGALLEGRAHQHLVDFDC 184

Query: 188 HLDDISKDWLNPDL 202
           HLDDI +DW N  L
Sbjct: 185 HLDDIRQDWTNQRL 191

BLAST of Spg012846 vs. ExPASy Swiss-Prot
Match: Q5U1W7 (ER membrane protein complex subunit 9 OS=Rattus norvegicus OX=10116 GN=Emc9 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.2e-22
Identity = 67/194 (34.54%), Postives = 102/194 (52.58%), Query Frame = 0

Query: 8   EISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLLPQLEISL 67
           EIS  AY K+ LHA ++  +AVNG+LL   +   + + + D VPLFHS + L   LE++L
Sbjct: 5   EISARAYGKMCLHASRYPHAAVNGLLLAPATRSGECLCLTDCVPLFHSHLALSVMLEVAL 64

Query: 68  LLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDHRKLEAL 127
             ++ +    GL + GY+HAN   DD   G +A  I   I+  FP+A L++LD++KL   
Sbjct: 65  NQVDVWATQAGLVVAGYYHANAVLDDQSPGPLALKIAGRIAEFFPNAVLIMLDNKKLVTW 124

Query: 128 PKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQDVVDFDD 187
           P+     PV+ L  +     W     D +  +      +  ++   +     Q +VDFD 
Sbjct: 125 PR---VPPVIVL--ENQGLQW--VPKDKNLVMWRDWEESRQMVGALLEGRAHQHLVDFDC 184

Query: 188 HLDDISKDWLNPDL 202
           HLDDI +DW N  L
Sbjct: 185 HLDDIRQDWTNQRL 191

BLAST of Spg012846 vs. ExPASy TrEMBL
Match: A0A5A7SKW9 (ER membrane protein complex subunit 8/9-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00760 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.1e-105
Identity = 194/203 (95.57%), Postives = 199/203 (98.03%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHR+SAVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRSSAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPVMELYTKETSK+WKLAGSDGSSQL IKEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. ExPASy TrEMBL
Match: A0A1S3C2C1 (ER membrane protein complex subunit 8/9 homolog OS=Cucumis melo OX=3656 GN=LOC103495860 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.1e-105
Identity = 194/203 (95.57%), Postives = 199/203 (98.03%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHR+SAVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRSSAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPVMELYTKETSK+WKLAGSDGSSQL IKEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. ExPASy TrEMBL
Match: A0A0A0KYR9 (MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419600 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 6.9e-105
Identity = 193/203 (95.07%), Postives = 199/203 (98.03%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRT+AVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTTAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPVMELYTKETSK+WKLAGSDGSSQL +KEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMMKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. ExPASy TrEMBL
Match: A0A6J1HRP6 (ER membrane protein complex subunit 8/9 homolog OS=Cucurbita maxima OX=3661 GN=LOC111466107 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.9e-103
Identity = 191/203 (94.09%), Postives = 198/203 (97.54%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGR SG NDV+EIA+SVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRISGSNDVIEIAESVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGLSIVGYFHAN RFDDY+VGGVAKNI DHISRSFPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANVRFDDYDVGGVAKNIADHISRSFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPV+ELYT+ETSK+WKLAGSDGSSQLT+KEPSANVVLLDFISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVVELYTRETSKNWKLAGSDGSSQLTMKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. ExPASy TrEMBL
Match: A0A6J1GG75 (ER membrane protein complex subunit 8/9 homolog OS=Cucurbita moschata OX=3662 GN=LOC111453869 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 3.8e-103
Identity = 189/203 (93.10%), Postives = 197/203 (97.04%), Query Frame = 0

Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKL+LHALKHRTSAVNG+LLGR SGP DV+EIADSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLILHALKHRTSAVNGLLLGRISGPTDVIEIADSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYY AKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180
           HRKLEALPKRKDRSPVMELYT+ET K+WKLAGSDGSSQLTIKEP AN+VLLD+ISSEKWQ
Sbjct: 121 HRKLEALPKRKDRSPVMELYTRETPKNWKLAGSDGSSQLTIKEPLANMVLLDYISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

BLAST of Spg012846 vs. TAIR 10
Match: AT5G55940.1 (Uncharacterised protein family (UPF0172) )

HSP 1 Score: 294.3 bits (752), Expect = 7.6e-80
Identity = 143/201 (71.14%), Postives = 177/201 (88.06%), Query Frame = 0

Query: 3   GDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPND-VVEIADSVPLFHSSIGLLP 62
           G+L++EISQNAYIKLVLH+L+H+T+AVNGVL+GR S  +D VVEI+DSVPLFHS++ LLP
Sbjct: 7   GELKYEISQNAYIKLVLHSLRHKTAAVNGVLVGRISPKDDGVVEISDSVPLFHSNLALLP 66

Query: 63  QLEISLLLIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDH 122
            LEISL++IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIGDHISR FP A +LLL++
Sbjct: 67  PLEISLIMIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIGDHISRYFPQAPILLLNN 126

Query: 123 RKLEALPKRKDRSPVMELYTKETSKHWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQD 182
           +KLEAL K K+RSPVM+L  K+ SK+W++ G+DG S+L +KEPSANVVL D+ISSEKW+D
Sbjct: 127 KKLEALSKGKERSPVMQLCVKDASKNWRVVGADGGSKLLLKEPSANVVLSDYISSEKWKD 186

Query: 183 VVDFDDHLDDISKDWLNPDLF 203
           V D DDHLDD++KDWLNP LF
Sbjct: 187 VTDVDDHLDDVTKDWLNPGLF 207

BLAST of Spg012846 vs. TAIR 10
Match: AT5G51620.3 (Uncharacterised protein family (UPF0172) )

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-16
Identity = 39/50 (78.00%), Postives = 44/50 (88.00%), Query Frame = 0

Query: 69  LIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLL 119
           +IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIGDHISR FP A +LL
Sbjct: 1   MIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIGDHISRYFPQAPILL 50

BLAST of Spg012846 vs. TAIR 10
Match: AT5G51620.2 (Uncharacterised protein family (UPF0172) )

HSP 1 Score: 63.2 bits (152), Expect = 2.9e-10
Identity = 29/36 (80.56%), Postives = 33/36 (91.67%), Query Frame = 0

Query: 69  LIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIG 105
           +IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIG
Sbjct: 1   MIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIG 36

BLAST of Spg012846 vs. TAIR 10
Match: AT5G51620.1 (Uncharacterised protein family (UPF0172) )

HSP 1 Score: 63.2 bits (152), Expect = 2.9e-10
Identity = 29/36 (80.56%), Postives = 33/36 (91.67%), Query Frame = 0

Query: 69  LIEEYYEAKGLSIVGYFHANERFDDYEVGGVAKNIG 105
           +IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIG
Sbjct: 1   MIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIG 36

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008455761.18.4e-10595.57PREDICTED: ER membrane protein complex subunit 8/9 homolog [Cucumis melo] >KAA00... [more]
XP_038880935.18.4e-10595.57ER membrane protein complex subunit 8/9 homolog [Benincasa hispida][more]
XP_004144311.11.4e-10495.07ER membrane protein complex subunit 8/9 homolog [Cucumis sativus] >KGN54668.1 hy... [more]
KAG6595444.12.7e-10394.09ER membrane protein complex subunit 8/9-like protein, partial [Cucurbita argyros... [more]
XP_023518172.16.0e-10394.09ER membrane protein complex subunit 8/9 homolog [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FG711.1e-7871.14ER membrane protein complex subunit 8/9 homolog OS=Arabidopsis thaliana OX=3702 ... [more]
Q5FVL22.9e-2330.88ER membrane protein complex subunit 8 OS=Rattus norvegicus OX=10116 GN=Emc8 PE=2... [more]
O703784.9e-2330.88ER membrane protein complex subunit 8 OS=Mus musculus OX=10090 GN=Emc8 PE=1 SV=1[more]
Q9DB766.4e-2335.05ER membrane protein complex subunit 9 OS=Mus musculus OX=10090 GN=Emc9 PE=1 SV=1[more]
Q5U1W73.2e-2234.54ER membrane protein complex subunit 9 OS=Rattus norvegicus OX=10116 GN=Emc9 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7SKW94.1e-10595.57ER membrane protein complex subunit 8/9-like protein OS=Cucumis melo var. makuwa... [more]
A0A1S3C2C14.1e-10595.57ER membrane protein complex subunit 8/9 homolog OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0KYR96.9e-10595.07MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419600 PE=3 SV... [more]
A0A6J1HRP62.9e-10394.09ER membrane protein complex subunit 8/9 homolog OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GG753.8e-10393.10ER membrane protein complex subunit 8/9 homolog OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G55940.17.6e-8071.14Uncharacterised protein family (UPF0172) [more]
AT5G51620.32.7e-1678.00Uncharacterised protein family (UPF0172) [more]
AT5G51620.22.9e-1080.56Uncharacterised protein family (UPF0172) [more]
AT5G51620.12.9e-1080.56Uncharacterised protein family (UPF0172) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005366ER membrane protein complex subunit 8/9PFAMPF03665UPF0172coord: 7..201
e-value: 1.2E-59
score: 201.7
IPR005366ER membrane protein complex subunit 8/9PANTHERPTHR12941ER MEMBRANE PROTEIN COMPLEXcoord: 1..203
IPR005366ER membrane protein complex subunit 8/9CDDcd08060MPN_UPF0172coord: 9..198
e-value: 8.27649E-72
score: 214.081
NoneNo IPR availablePANTHERPTHR12941:SF14ER MEMBRANE PROTEIN COMPLEX SUBUNIT 8/9 HOMOLOGcoord: 1..203
IPR037518MPN domainPROSITEPS50249MPNcoord: 7..146
score: 18.092991

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012846.1Spg012846.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0072546 EMC complex