Spg012737 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012737
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionThreonine dehydratase
Locationscaffold1: 12365846 .. 12370308 (-)
RNA-Seq ExpressionSpg012737
SyntenySpg012737
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAATGCTTCACACAAAGCAAGAAGTAATCAACAACAACAAAAACAAAACAAACTTCAACTTCCTTGTTCTTTCTTTCTTTTACTACAATAATGGAGTCCTTACTCCTCACCACCACCACTTCAAACCTGCTGACCGGCCGAAACTCTCCGGTGTCGTCGCTGTCTTCCTCTTCTTCCTTGGGCTCGATTCCGAGTGGCAAGGTCGAGGTCAGGAGAAGCAGAATTGGAAAGGTTAAGACACCGACGTCCATAGTGGCATCGGTGTCGAAACGGGGGACCGGTAAGGGGACTGCCAATGTGGCAGTGGACGGTTCGGTTGCGGCGGTCGCGGCAGATGTCCCGGATGTGTCGTGGACGGAAGTGCAGTACCCGGCAGGGTCAATCGGACACAGGCATCCGAAGCCGACGGAGGTTGATAAAGAGAAACAGATGGGGTATATTACAAAAATATTGGGTTCCACAGTGTACGATGTGGCTGTTGAAACGCCCTTGCAGCTTGCTCCTCTCTTGTCCCAGAAGTTGGGGGTCAATCTCTGGCTCAAGAGGGAGGATTCGCAGCAGGTAACTATACCATTTTGCTTTGCTTTTGCTTTTGCTTTTGCTTTTCAGTTTTTCTCTTTCTTCATTTCTGTTTTTTTTTTTTTTAAATTACAAATTTAGTATTAATCATTTAACTTTTGATGGGAGTAATGTTTAATTATAAGCTTAATTTTCAAAAATAAAAAACTAAATGATTATCAAATGTGACCTTAACTCTCAATTTTGTGTATAATAGATCTCCAAACTTGACAAATGCATGATAAATCCCTTAACAAGTTCTTGTCTAATTGAAATTTAAATTTTATCAAACTTACTCGGGTGTTGGATAATAATATTTTTGGATATATACGTAGGTATTCTCATTCAAGATTCGAGGAGCTTATAATATGATTTCCCAACTTCCAGAAGATCAACGGAAGAAAGGAGTTATCTGCGCGTCAGCTGGGAATCATGCCCAAGGAGTTGCATTATCTGCTAAGCGATTAAAAATTGTAGCCAACATTGTTATGCCAACAACTACACCCCAAATTAAGGTGCACTAATTAATTAACATCATATATATATATATATATATATATATAGCTTTTAGCTTTTAGCTTTCATTTTTATTAATAATATTGGAAACTTGTCATATATATCACACTCACAGATAGATGCAGTTAAGAGATTGGGTGGAGCGGTTGAGACGTGGGGCGATACTTTTGATAAAGCACAAGAAAGAGCTAAACAGCGAGCCATAGACGAAGACTTAACATTCATACCTCCTTTTGATGACGAACATGTCATAGCTGGCCAGGGTACTGTCGGCATGGAAATTTCCCGTCAAATGAAGGGCAACATCCATGCCATGTTTGTGCCCGTTGGTGGAGGTGGTCTCATTTCCGGTATCGCCTCTTTCTACAAGTTGGTTTATCCCGGGGTGAGTTCTATCGCTTTTAACCCATTTACCCTATACTACTCCATTTTACATCAACTCAGCGCTCTGTTTGGAATGACTTTCTAACTGTTTCAAACACAGGTCAAGATTTTTGGAGTCGAGCCCAATGACGAAAACTCCATGGCACAAGCGTTAAAGCGCGGCTACATAGTGAATGTGACAGATATTGGACATTTTGCAGATGGTGTAGCTGTTCAACAGGTTGGCAATGAGACTTTCCGAATTTGCAACGAGGTCGTCGACGATATTATTCTCGTCGACAAATATGCAATATCTAAGGCAGTTCAGGTACAAATTGAGATATATATATTGACATGATCTCTATGTATGCAGTCAAAACTAATTTGTTTTTGAGGTGTACTGATAGGATATGTTTAACGACGGGAGGAACATCTTGGAACCTTCGGGTGCGCTATCAATTGCTGCAGCCAAGGCATATTGCGAATACAACAATATTAAAGGAGTAAACGTTGTGGCAGTAACCAGTGGCGCCAATATGAACTTCGACCAATTGCGTGTAATTTCTGATATCGCCAATAATGATACTCAAACGATTCTTGTATCTATATTGCCAGAGGTGCCTGGCAGCTTAGATAAGCTCACAAAGCTGATCGATGAAAAGGGCTTCAACATTCTAGAAACCAGTTACAGATTTGGCGAAGGCTCAACGGATGCTGTTGTTGTTTATAAGTAAGTGTTTTTTTAATACACCTCTCAATTAATACTTTTCCATCATATATTTAATTAATTTTTCATCACTGTGCTGTGCAGGGTGGATGCAGTAGGCTCAGATCTTGAAAATTTGTTGCTGGATTTGAATTCTTTTGGCTATGAAACTTACGCTTTGAATGATAATGATGCTGCCGCGAGTCACCTCCGTTACATGGTAAATATATCTTTTTTTTTTTTTTCTTATTGAATTTGGAAAATGCCTGCTTAATTAATTTCTAATCAACATGTATATATATTTTTTCTTCCTTTCAGACGGGAGGCAGAGCAAAGGTTGAAAACGAAACTCTCTTCCGTTTCAGTTTCCCAGAAAGGAAAGGTGCTCTCAAGCATTTCCTCCATGGCTTTCAGCCCAATTGGAACATTTCTTTGTTCCACCATCGTGCAGAGGTAAGTCCCGTTTGTTAACCATTTTGATCATTTTCGAAGATTATCAAGAAAAGCCACTTGTTAATTTATCTTTTTTTTTTCTTTTTTTCAACAGGGTATTCTTAAATCAAGTGTATTAGTTGGAATTCAACTGGAAAAATCAGAGGAGAAAAATTTCCATGCTTATGCAAAGAAGGTGGGGTATAAATACGAAGTATTATCTCCGGATGATGCGGCCTATATTCTAACTTCAAAACCCTAGATGCGGCCGGCGGAGGCTTGCGATTCATTTCACTCAACAAAGTCAGCTTAATTAGTGGAATAAATTACTCGAAATATGTTGGTGGTAATGTAATTTTAACTTACAAAATTAGCTAGCTAGCTAGGGGTTGTTGTTGGTGTGTTTGATGTTGGAATAAACACTTTGTTTGATCTCTCTCTATAAATTTGATCAAACAGAAAGAAAAGATGATGGAAGTTAGTGAAATGGAGTTGCTCTTATGTATTGGCGAGATAATTGCCAACTATCTACATATATATTGTATAAAATAAAGTATAATGCAAGTGGTTTTCTAAAACCACATGCTTTTCGCTTATTTTGTGCTTAAATTAAAGCATTTTTATTCTCGTAATTCACAAGTAATGTTGACAAAAATGAATGGTTTGCCACTCAACTCGCATAAGATTTGCACTGATAATCGAATGGTCCAGTGACTAAAAAAAACAAACAATTGGTTGACATTTCATGGAATGGATAAAAAAAGAAAAGGAATAAGTTAAATTACAAGTTTAGTTCTTAAACTTTCAAGATTGTGAAATAGGGTCTTTGAATTTTTAGTTTTGTGTCCAATAATTAAGTCAACTTCAACGCTTTCAATTTTGTATCTATAATTAGTTCTTGACCTATTCAACATTTTAAAAAGTTCACAAGTCTACTAGATGCAAAATTGGAAGTTTATGGTCCCAATAGGTATAAAATTCAATTTGATATCTAATAGATTAGCTAATTTTTAAAAATCTCGAATATGCCAGGGTTCTATTAGACACAAAATTGAAAGTTCAGGAATTTATTAGACGTTTTTAAAAGTTTCAGGACCTAATTGATACAATCTTAAAAGTTTAGGAACCAAACTTATAAAAAAAGCGAAAAGAATTATGTCCTTACAAAATTTGTCAAAATGACCTCAAATCTAAATTAAAGTTAAATGCCCTCTCGTTTGGACAAATATGCCCTGTCTGCTTTCATCCTCTATTCAACCCAACTTTTTCTTCTTCCTTCCTTGGCTATTCAATTGGTATAATCTAATAAGTATTATGGGATTTAGCTTTCCTATATTATTGCTGATTATGAATCAATTTCACTCTCAATGTTGTTTAACTGTTGCTGTACTGAAACAAGGGATTTTGAATTACACAGTTTGGTATTACCGGCCAGTCTTCTTTGAAATCCAAAAGTGACAGAGTGGTCTTGATGCCACGATCCACTGAGATTCTAACCCTTCGTCGCTTTGATTTTTTTTTATTGTTGGGAGTGCGACCAAAGTTTCACATTGGCTAGATAAGGGGATGATCATGGGTATATAAGGGAGGACAAGTATCTCCATTATGAGGCTTTTTGAGGTGAACCAAAAGTAAAGTCATGAGGGGCTTATGCCCAAAGTGGACAACATCATATCATTGTGGAGATATGTGGTTGTCGAGTTGTCCCTTACAAGTGGTATTAGAGCCATGCATGGAAGTAGCTATGTTGGGTGGAATCTTCGAGTGTCGAACAAAGAAGTTGTGAGCCTTAATAGTCATGGTGGAGGTCCTGTGGTTGAGCTCTGGGTAGGCGGGTATACTCCGGTGAAAGGAGTTGTCCTGGATAAGAGCGAGAACTCTCGATAA

mRNA sequence

ATGGAGTCCTTACTCCTCACCACCACCACTTCAAACCTGCTGACCGGCCGAAACTCTCCGGTGTCGTCGCTGTCTTCCTCTTCTTCCTTGGGCTCGATTCCGAGTGGCAAGGTCGAGGTCAGGAGAAGCAGAATTGGAAAGGTTAAGACACCGACGTCCATAGTGGCATCGGTGTCGAAACGGGGGACCGGTAAGGGGACTGCCAATGTGGCAGTGGACGGTTCGGTTGCGGCGGTCGCGGCAGATGTCCCGGATGTGTCGTGGACGGAAGTGCAGTACCCGGCAGGGTCAATCGGACACAGGCATCCGAAGCCGACGGAGGTTGATAAAGAGAAACAGATGGGGTATATTACAAAAATATTGGGTTCCACAGTGTACGATGTGGCTGTTGAAACGCCCTTGCAGCTTGCTCCTCTCTTGTCCCAGAAGTTGGGGGTCAATCTCTGGCTCAAGAGGGAGGATTCGCAGCAGGTATTCTCATTCAAGATTCGAGGAGCTTATAATATGATTTCCCAACTTCCAGAAGATCAACGGAAGAAAGGAGTTATCTGCGCGTCAGCTGGGAATCATGCCCAAGGAGTTGCATTATCTGCTAAGCGATTAAAAATTGTAGCCAACATTGTTATGCCAACAACTACACCCCAAATTAAGATAGATGCAGTTAAGAGATTGGGTGGAGCGGTTGAGACGTGGGGCGATACTTTTGATAAAGCACAAGAAAGAGCTAAACAGCGAGCCATAGACGAAGACTTAACATTCATACCTCCTTTTGATGACGAACATGTCATAGCTGGCCAGGGTACTGTCGGCATGGAAATTTCCCGTCAAATGAAGGGCAACATCCATGCCATGTTTGTGCCCGTTGGTGGAGGTGGTCTCATTTCCGGTATCGCCTCTTTCTACAAGTTGGTTTATCCCGGGGTCAAGATTTTTGGAGTCGAGCCCAATGACGAAAACTCCATGGCACAAGCGTTAAAGCGCGGCTACATAGTGAATGTGACAGATATTGGACATTTTGCAGATGGTGTAGCTGTTCAACAGGTTGGCAATGAGACTTTCCGAATTTGCAACGAGGTCGTCGACGATATTATTCTCGTCGACAAATATGCAATATCTAAGGCAGTTCAGGATATGTTTAACGACGGGAGGAACATCTTGGAACCTTCGGGTGCGCTATCAATTGCTGCAGCCAAGGCATATTGCGAATACAACAATATTAAAGGAGTAAACGTTGTGGCAGTAACCAGTGGCGCCAATATGAACTTCGACCAATTGCGTGTAATTTCTGATATCGCCAATAATGATACTCAAACGATTCTTGTATCTATATTGCCAGAGGTGCCTGGCAGCTTAGATAAGCTCACAAAGCTGATCGATGAAAAGGGCTTCAACATTCTAGAAACCAGTTACAGATTTGGCGAAGGCTCAACGGATGCTGTTGTTGTTTATAAGGTGGATGCAGTAGGCTCAGATCTTGAAAATTTGTTGCTGGATTTGAATTCTTTTGGCTATGAAACTTACGCTTTGAATGATAATGATGCTGCCGCGAGTCACCTCCGTTACATGACGGGAGGCAGAGCAAAGGTTGAAAACGAAACTCTCTTCCGTTTCAGTTTCCCAGAAAGGAAAGGTGCTCTCAAGCATTTCCTCCATGGCTTTCAGCCCAATTGGAACATTTCTTTGTTCCACCATCGTGCAGAGGGTATTCTTAAATCAAGTGTATTAGTTGGAATTCAACTGGAAAAATCAGAGGAGAAAAATTTCCATGCTTATGCAAAGAAGGTGGGTCATGAGGGGCTTATGCCCAAAGTGGACAACATCATATCATTGTGGAGATATGTGGTTGTCGAGTTGTCCCTTACAAGTGGTATTAGAGCCATGCATGGAAGTAGCTATGTTGGGTGGAATCTTCGAGTGTCGAACAAAGAAGTTGTGAGCCTTAATAGTCATGGTGGAGGTCCTGTGGTTGAGCTCTGGGTAGGCGGGTATACTCCGGTGAAAGGAGTTGTCCTGGATAAGAGCGAGAACTCTCGATAA

Coding sequence (CDS)

ATGGAGTCCTTACTCCTCACCACCACCACTTCAAACCTGCTGACCGGCCGAAACTCTCCGGTGTCGTCGCTGTCTTCCTCTTCTTCCTTGGGCTCGATTCCGAGTGGCAAGGTCGAGGTCAGGAGAAGCAGAATTGGAAAGGTTAAGACACCGACGTCCATAGTGGCATCGGTGTCGAAACGGGGGACCGGTAAGGGGACTGCCAATGTGGCAGTGGACGGTTCGGTTGCGGCGGTCGCGGCAGATGTCCCGGATGTGTCGTGGACGGAAGTGCAGTACCCGGCAGGGTCAATCGGACACAGGCATCCGAAGCCGACGGAGGTTGATAAAGAGAAACAGATGGGGTATATTACAAAAATATTGGGTTCCACAGTGTACGATGTGGCTGTTGAAACGCCCTTGCAGCTTGCTCCTCTCTTGTCCCAGAAGTTGGGGGTCAATCTCTGGCTCAAGAGGGAGGATTCGCAGCAGGTATTCTCATTCAAGATTCGAGGAGCTTATAATATGATTTCCCAACTTCCAGAAGATCAACGGAAGAAAGGAGTTATCTGCGCGTCAGCTGGGAATCATGCCCAAGGAGTTGCATTATCTGCTAAGCGATTAAAAATTGTAGCCAACATTGTTATGCCAACAACTACACCCCAAATTAAGATAGATGCAGTTAAGAGATTGGGTGGAGCGGTTGAGACGTGGGGCGATACTTTTGATAAAGCACAAGAAAGAGCTAAACAGCGAGCCATAGACGAAGACTTAACATTCATACCTCCTTTTGATGACGAACATGTCATAGCTGGCCAGGGTACTGTCGGCATGGAAATTTCCCGTCAAATGAAGGGCAACATCCATGCCATGTTTGTGCCCGTTGGTGGAGGTGGTCTCATTTCCGGTATCGCCTCTTTCTACAAGTTGGTTTATCCCGGGGTCAAGATTTTTGGAGTCGAGCCCAATGACGAAAACTCCATGGCACAAGCGTTAAAGCGCGGCTACATAGTGAATGTGACAGATATTGGACATTTTGCAGATGGTGTAGCTGTTCAACAGGTTGGCAATGAGACTTTCCGAATTTGCAACGAGGTCGTCGACGATATTATTCTCGTCGACAAATATGCAATATCTAAGGCAGTTCAGGATATGTTTAACGACGGGAGGAACATCTTGGAACCTTCGGGTGCGCTATCAATTGCTGCAGCCAAGGCATATTGCGAATACAACAATATTAAAGGAGTAAACGTTGTGGCAGTAACCAGTGGCGCCAATATGAACTTCGACCAATTGCGTGTAATTTCTGATATCGCCAATAATGATACTCAAACGATTCTTGTATCTATATTGCCAGAGGTGCCTGGCAGCTTAGATAAGCTCACAAAGCTGATCGATGAAAAGGGCTTCAACATTCTAGAAACCAGTTACAGATTTGGCGAAGGCTCAACGGATGCTGTTGTTGTTTATAAGGTGGATGCAGTAGGCTCAGATCTTGAAAATTTGTTGCTGGATTTGAATTCTTTTGGCTATGAAACTTACGCTTTGAATGATAATGATGCTGCCGCGAGTCACCTCCGTTACATGACGGGAGGCAGAGCAAAGGTTGAAAACGAAACTCTCTTCCGTTTCAGTTTCCCAGAAAGGAAAGGTGCTCTCAAGCATTTCCTCCATGGCTTTCAGCCCAATTGGAACATTTCTTTGTTCCACCATCGTGCAGAGGGTATTCTTAAATCAAGTGTATTAGTTGGAATTCAACTGGAAAAATCAGAGGAGAAAAATTTCCATGCTTATGCAAAGAAGGTGGGTCATGAGGGGCTTATGCCCAAAGTGGACAACATCATATCATTGTGGAGATATGTGGTTGTCGAGTTGTCCCTTACAAGTGGTATTAGAGCCATGCATGGAAGTAGCTATGTTGGGTGGAATCTTCGAGTGTCGAACAAAGAAGTTGTGAGCCTTAATAGTCATGGTGGAGGTCCTGTGGTTGAGCTCTGGGTAGGCGGGTATACTCCGGTGAAAGGAGTTGTCCTGGATAAGAGCGAGAACTCTCGATAA

Protein sequence

MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSKRGTGKGTANVAVDGSVAAVAADVPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYITKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQRKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICNEVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGANMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGSTDAVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRFSFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGHEGLMPKVDNIISLWRYVVVELSLTSGIRAMHGSSYVGWNLRVSNKEVVSLNSHGGGPVVELWVGGYTPVKGVVLDKSENSR
Homology
BLAST of Spg012737 vs. NCBI nr
Match: KAG6604380.1 (Threonine dehydratase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 842.4 bits (2175), Expect = 2.7e-240
Identity = 439/601 (73.04%), Postives = 501/601 (83.36%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSK 60
           MES+LLT TTSNL   RNS +S LS SSSL +  S KV++RR+  G  K P  + +S   
Sbjct: 1   MESILLTATTSNLPLPRNSSLSPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRIVASSSGS 60

Query: 61  RG-TGKG-TANVAVDGSVAAVAAD-VPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYI 120
           +G  GKG +ANVAVDGSVAAVAA+ V DVSW E+QY  GS GHR P PT  D EKQM YI
Sbjct: 61  KGRAGKGVSANVAVDGSVAAVAAEGVKDVSWKELQYEEGSTGHRQPLPTNPDTEKQMEYI 120

Query: 121 TKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQ 180
           TKILGS VYDVA+ETPLQLAPLLS +LGVNLWLKREDSQQVFSFKIRGAYNMISQLPE+Q
Sbjct: 121 TKILGSNVYDVAIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEEQ 180

Query: 181 RKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDK 240
            KKG+ICASAGNHAQGVALS +RLK  A IVMPTTTPQIKIDAV+RLGG V+  G++FD+
Sbjct: 181 LKKGIICASAGNHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLHGNSFDE 240

Query: 241 AQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGI 300
           AQ+RAK+R+ +E LTFIPPFDD+ VIAGQGTVGMEI RQ++G IHA+FVP+GGGGL SGI
Sbjct: 241 AQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGI 300

Query: 301 ASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICN 360
            SFYKLVYP VK+FGVEPND+NSMAQAL RG IVNVTDIGHFADGVAVQQVG ETFRIC 
Sbjct: 301 VSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICY 360

Query: 361 EVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG 420
           E++DD+ILV K +IS A++DMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG
Sbjct: 361 ELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG 420

Query: 421 ANMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGST 480
           ANMNFDQL  ISD+AN D Q+IL ++LPE PGSL +LT LI + G  I E +YRF  GST
Sbjct: 421 ANMNFDQLGQISDLANID-QSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGST 480

Query: 481 DAVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRF 540
           DA+VVYKV AV S LE L+  LN FG++TY L+DN+   +HLRYM GGR  VENETLFRF
Sbjct: 481 DALVVYKVKAVDSALETLVRQLNFFGFKTYTLSDNEVVRNHLRYMMGGRQNVENETLFRF 540

Query: 541 SFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGH 599
           +FPE+ GALKHFL  FQPNWN+SLFHHR +GIL S VL+G+QLEKSEEK FH YAK+VG+
Sbjct: 541 TFPEKVGALKHFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFHEYAKRVGY 600

BLAST of Spg012737 vs. NCBI nr
Match: KAG7034531.1 (Threonine dehydratase biosynthetic, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 840.5 bits (2170), Expect = 1.0e-239
Identity = 439/601 (73.04%), Postives = 500/601 (83.19%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSK 60
           MES+LLT TTSNL   RNS +S LS SSSL +  S KV++RR+  G  K P  + +S   
Sbjct: 1   MESILLTATTSNLPLPRNSSLSPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRIVASSSGS 60

Query: 61  RG-TGKG-TANVAVDGSVAAVAAD-VPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYI 120
           +G  GKG +ANVAVDGSVAAVAA+ V DVSW E+QY  GS GHR P PT  D EKQM YI
Sbjct: 61  KGRAGKGVSANVAVDGSVAAVAAEGVKDVSWKELQYEEGSTGHRQPLPTNPDTEKQMEYI 120

Query: 121 TKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQ 180
           TKILGS VYDVA+ETPLQLAPLLS +LGVNLWLKREDSQQVFSFKIRGAYNMISQLPE+Q
Sbjct: 121 TKILGSNVYDVAIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEEQ 180

Query: 181 RKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDK 240
            KKG+ICASAGNHAQGVALS +RLK  A+IVMPTTTPQIKIDAV+RLGG V+  G+TFD+
Sbjct: 181 LKKGIICASAGNHAQGVALSGQRLKTKAHIVMPTTTPQIKIDAVERLGGIVDLKGNTFDE 240

Query: 241 AQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGI 300
           AQ+ AK+R+  E LTFIPPFDD+ VIAGQGTVGMEI RQ++G IHA+FVP+GGGGL SGI
Sbjct: 241 AQKIAKERSEKEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGI 300

Query: 301 ASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICN 360
            SFYKLVYP VK+FGVEPND+NSMAQAL RG IVNVTDIGHFADGVAVQQVG ETFRIC 
Sbjct: 301 VSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICY 360

Query: 361 EVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG 420
           E++DD+ILV K +IS A++DMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG
Sbjct: 361 ELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG 420

Query: 421 ANMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGST 480
           ANMNFDQL  ISD+AN D Q+IL ++LPE PGSL +LT LI + G  I E +YRF  GST
Sbjct: 421 ANMNFDQLGQISDLANID-QSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGST 480

Query: 481 DAVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRF 540
           DA+VVYKV AV S LE L+  LN FG++TY L+DN+   +HLRYM GGR  VENETLFRF
Sbjct: 481 DALVVYKVKAVDSALETLVRQLNFFGFKTYTLSDNEVVRNHLRYMMGGRQNVENETLFRF 540

Query: 541 SFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGH 599
           +FPE+ GALKHFL  FQPNWN+SLFHHR +GIL S VL+G+QLEKSEEK FH YAK+VG+
Sbjct: 541 TFPEKVGALKHFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFHEYAKRVGY 600

BLAST of Spg012737 vs. NCBI nr
Match: XP_023543427.1 (threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 839.3 bits (2167), Expect = 2.3e-239
Identity = 438/601 (72.88%), Postives = 499/601 (83.03%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSK 60
           MES+LLT  TSNL   RNS +S LS SSSL +  S KV++RR+  G  K P  + +S   
Sbjct: 1   MESILLTAATSNLPLPRNSSLSPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRIVASSSGS 60

Query: 61  RG-TGKG-TANVAVDGSVAAVAAD-VPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYI 120
           +G  GKG +ANVAVDGSVAAVAA+ V DVSW E+QY  GS GHR P PT  D EKQM YI
Sbjct: 61  KGRAGKGVSANVAVDGSVAAVAAEGVKDVSWKELQYEEGSTGHRQPLPTNPDTEKQMEYI 120

Query: 121 TKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQ 180
           TKILGS VYDVA+ETPLQLAPLLS +LGVNLWLKREDSQQVFSFKIRGAYNMISQLPE+Q
Sbjct: 121 TKILGSNVYDVAIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEEQ 180

Query: 181 RKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDK 240
            KKG+ICASAGNHAQGVALS +RLK  A+IVMPTTTPQIKIDAV+RLGG V+  G+TFD+
Sbjct: 181 LKKGIICASAGNHAQGVALSGQRLKTKAHIVMPTTTPQIKIDAVERLGGIVDLKGNTFDE 240

Query: 241 AQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGI 300
           AQ+ AK+R+  E LTFIPPFDD+ VIAGQGTVGMEI RQ++G IHA+FVP+GGGGL SGI
Sbjct: 241 AQKIAKERSEKEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGI 300

Query: 301 ASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICN 360
            SFYKLVYP VK+FGVEPND+NSMAQAL RG IVNVTDIGHFADGVAVQQVG ETFRIC 
Sbjct: 301 VSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICY 360

Query: 361 EVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG 420
           E++DD+ILV K +IS A++DMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG
Sbjct: 361 ELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG 420

Query: 421 ANMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGST 480
           ANMNFDQL  ISD+AN D Q+IL ++LPE PGSL +LT LI + G  + E +YRF  GST
Sbjct: 421 ANMNFDQLGQISDLANID-QSILATMLPETPGSLKQLTDLIADLGVTVTEMTYRFSSGST 480

Query: 481 DAVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRF 540
           DA+VVYKV AV S LE L+  LN FG++TY L+DN+   +HLRYM GGR  VENETLFRF
Sbjct: 481 DALVVYKVKAVDSALETLVRQLNFFGFKTYTLSDNEVVRNHLRYMMGGRQNVENETLFRF 540

Query: 541 SFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGH 599
           +FPE+ GALKHFL  FQPNWN+SLFHHR +GIL S VL+G+QLEKSEEK FH YAKKVG+
Sbjct: 541 TFPEKVGALKHFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFHEYAKKVGY 600

BLAST of Spg012737 vs. NCBI nr
Match: XP_022977240.1 (threonine dehydratase biosynthetic, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 836.3 bits (2159), Expect = 1.9e-238
Identity = 436/600 (72.67%), Postives = 499/600 (83.17%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSK 60
           MES+LLT TTSN+   RNS +SSLS SSSL +  S KVE+RRS  G  K P  + +S   
Sbjct: 1   MESILLTATTSNIPLPRNSSLSSLSFSSSLPNQVSTKVEIRRSNKGTTKAPRIVASSSGS 60

Query: 61  RG-TGKG-TANVAVDGSVAAVAADVPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYIT 120
           +G  GKG +ANVAVDGSV  VA  V DVSW E+QY  GS GHR P PT+ D EKQM YIT
Sbjct: 61  KGRAGKGVSANVAVDGSV--VAEGVKDVSWKELQYEEGSTGHRLPLPTDPDTEKQMEYIT 120

Query: 121 KILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQR 180
            ILGS VYDVA+ETPLQLAPLLS +LGVNLWLKREDSQQVFSFKIRGAYNMIS+LPE Q 
Sbjct: 121 NILGSNVYDVAIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISKLPEKQL 180

Query: 181 KKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDKA 240
           KKG+ICASAGNHAQGVALS +RLK  A IVMPTTTPQIKIDAV+RLGG V+ +G++FD+A
Sbjct: 181 KKGIICASAGNHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLYGNSFDEA 240

Query: 241 QERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGIA 300
           Q+RAK+R+ +E LTFIPPFDD+ VIAGQGTVGMEI RQ++G IHA+FVP+GGGGL SGI 
Sbjct: 241 QKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIV 300

Query: 301 SFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICNE 360
           SFYKLVYP VK+FGVEPND+NSMAQAL RG IVNVTDIGHFADGVAVQQVG+ETFRIC E
Sbjct: 301 SFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGDETFRICYE 360

Query: 361 VVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA 420
           ++DD+ILV K +IS A++DMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA
Sbjct: 361 LLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA 420

Query: 421 NMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGSTD 480
           NMNFDQL  ISD+AN D Q+IL ++LPE PGSL +LT LI + G  I E +YRF  GSTD
Sbjct: 421 NMNFDQLGQISDLANID-QSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGSTD 480

Query: 481 AVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRFS 540
           A+VVYKV AV S LE L+  LN FG++TY L+DN+   +HLR+M GGR  VENETLFRF+
Sbjct: 481 ALVVYKVKAVDSALETLVRQLNFFGFKTYTLSDNEVVRNHLRFMMGGRQNVENETLFRFT 540

Query: 541 FPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGHE 599
           FPE+ GALKHFL  FQPNWN+SLFHHR +GIL S VL+G+QLEKSEEK FH YAKKVG++
Sbjct: 541 FPEKVGALKHFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFHEYAKKVGYQ 597

BLAST of Spg012737 vs. NCBI nr
Match: XP_022977241.1 (threonine dehydratase biosynthetic, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 823.9 bits (2127), Expect = 9.9e-235
Identity = 433/600 (72.17%), Postives = 494/600 (82.33%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSK 60
           MES+LLT TTSN+   RNS +SSLS SSSL +  S KVE+RRS  G  K P  + +S   
Sbjct: 1   MESILLTATTSNIPLPRNSSLSSLSFSSSLPNQVSTKVEIRRSNKGTTKAPRIVASSSGS 60

Query: 61  RG-TGKG-TANVAVDGSVAAVAADVPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYIT 120
           +G  GKG +ANVAVDGSV  VA  V DVSW E+QY  GS GHR P PT+ D EKQM YIT
Sbjct: 61  KGRAGKGVSANVAVDGSV--VAEGVKDVSWKELQYEEGSTGHRLPLPTDPDTEKQMEYIT 120

Query: 121 KILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQR 180
            ILGS VYDVA+ETPLQLAPLLS +LGVNLWLKREDSQQVFSFKIRGAYNMIS+LPE Q 
Sbjct: 121 NILGSNVYDVAIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISKLPEKQL 180

Query: 181 KKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDKA 240
           KKG+ICASAGNHAQGVALS +RLK  A IVMPTTTPQIKIDAV+RLGG V+ +G++FD+A
Sbjct: 181 KKGIICASAGNHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLYGNSFDEA 240

Query: 241 QERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGIA 300
           Q+RAK+R+ +E LTFIPPFDD+ VIAGQGTVGMEI RQ++G IHA+FVP+GGGGL SGI 
Sbjct: 241 QKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIV 300

Query: 301 SFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICNE 360
           SFYKLVYP VK+FGVEPND+NSMAQAL RG IVNVTDIGHFADGVAVQQVG+ETFRIC E
Sbjct: 301 SFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGDETFRICYE 360

Query: 361 VVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA 420
           ++DD+ILV K +IS A++DMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA
Sbjct: 361 LLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA 420

Query: 421 NMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGSTD 480
           NMNFDQL  ISD+AN D Q+IL ++LPE PGSL +LT LI + G  I E +YRF  GSTD
Sbjct: 421 NMNFDQLGQISDLANID-QSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGSTD 480

Query: 481 AVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRFS 540
           A+VVYKV AV S LE L+  LN FG++TY L+DN+   +HLR+M GGR  VENETLFRF+
Sbjct: 481 ALVVYKVKAVDSALETLVRQLNFFGFKTYTLSDNEVVRNHLRFMMGGRQNVENETLFRFT 540

Query: 541 FPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGHE 599
           FPE+ GAL HFL GFQPNWNISL HH   GI+ S VL+GIQLEKS EK F  YAKKVG++
Sbjct: 541 FPEKAGALNHFLKGFQPNWNISLLHHPDLGIITSDVLIGIQLEKSLEKKFDEYAKKVGYQ 597

BLAST of Spg012737 vs. ExPASy Swiss-Prot
Match: A0FKE6 (Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD1 PE=1 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 8.1e-147
Identity = 268/498 (53.82%), Postives = 352/498 (70.68%), Query Frame = 0

Query: 116 YITKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPE 175
           Y+T IL S VYDVA ETPLQ AP LS++LGVN+WLKRED Q VFSFKIRGAYNM+++LP+
Sbjct: 108 YLTNILSSKVYDVAYETPLQKAPKLSERLGVNVWLKREDLQPVFSFKIRGAYNMMAKLPK 167

Query: 176 DQRKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTF 235
           +Q +KGVIC+SAGNHAQGVALSA+RL   A IVMP TTP IK  +VKRLG  V   GD++
Sbjct: 168 EQLEKGVICSSAGNHAQGVALSAQRLGCDAVIVMPVTTPDIKWKSVKRLGATVVLVGDSY 227

Query: 236 DKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLIS 295
           D+AQ  AK+RA  E  TFIPPFD   VI GQGTVGMEI+RQ+K NIHA+FVPVGGGGLI+
Sbjct: 228 DEAQAYAKKRAESEGRTFIPPFDHPDVIVGQGTVGMEINRQLKDNIHAIFVPVGGGGLIA 287

Query: 296 GIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRI 355
           GIA++ K V P +KI GVEP D N++A +L  G  V +  +G FADGVAV+ VG ET+R+
Sbjct: 288 GIAAYLKRVAPDIKIIGVEPLDANALALSLHHGQRVMLDQVGGFADGVAVKVVGEETYRL 347

Query: 356 CNEVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVT 415
           C E++D ++LV + AI  +++DMF + R+ILEP+GAL++A A+AYC+Y  +KG NVVA+T
Sbjct: 348 CEELIDGVVLVGRDAICASIKDMFEEKRSILEPAGALALAGAEAYCKYYGLKGENVVAIT 407

Query: 416 SGANMNFDQLRVISDIAN--NDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFG 475
           SGANMNFD+LR+++++A+     + +L + +PE PGS  K  +++     NI E  YR+ 
Sbjct: 408 SGANMNFDRLRLVTELADVGRQREAVLATFMPEDPGSFKKFAEMVGP--MNITEFKYRYN 467

Query: 476 EGSTDAVVVYKVDA-VGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENE 535
                A+V+Y V      +LE ++  + S   +T  L DND    HLR++ GGR  V NE
Sbjct: 468 SDKERALVLYSVGLHTILELEGMVERMESADLQTINLTDNDLVKDHLRHLMGGRTNVHNE 527

Query: 536 TLFRFSFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYA 595
            L RF+FPE+ GAL  FL  F P WNISLFH+RA+G   ++VLVGIQ+   E   F   A
Sbjct: 528 LLCRFTFPEKPGALMKFLDAFSPRWNISLFHYRAQGDTGANVLVGIQVPPDEVVEFEGRA 587

Query: 596 KKVGHEGLMPKVDNIISL 611
             +G+E  M  ++    L
Sbjct: 588 DSLGYEYAMESLNEAYQL 603

BLAST of Spg012737 vs. ExPASy Swiss-Prot
Match: Q9ZSS6 (Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OMR1 PE=1 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.2e-145
Identity = 274/531 (51.60%), Postives = 367/531 (69.11%), Query Frame = 0

Query: 86  VSWTEVQYPAGSIGHRHPKPTEVDK---EKQMGYITKILGSTVYDVAVETPLQLAPLLSQ 145
           VS   +QYPAG +G    +  E +     + M Y+T IL + VYD+A+E+PLQLA  LS+
Sbjct: 62  VSPNSLQYPAGYLGAVPERTNEAENGSIAEAMEYLTNILSTKVYDIAIESPLQLAKKLSK 121

Query: 146 KLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQRKKGVICASAGNHAQGVALSAKRLK 205
           +LGV ++LKRED Q VFSFK+RGAYNM+ +LP DQ  KGVIC+SAGNHAQGVALSA +L 
Sbjct: 122 RLGVRMYLKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLG 181

Query: 206 IVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDKAQERAKQRAIDEDLTFIPPFDDEHV 265
             A IVMP TTP+IK  AV+ LG  V  +GD++D+AQ  AK RA +E LTFIPPFD   V
Sbjct: 182 CTAVIVMPVTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDV 241

Query: 266 IAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGIASFYKLVYPGVKIFGVEPNDENSMA 325
           IAGQGTVGMEI+RQ KG +HA+FVPVGGGGLI+GIA++ K V P VKI GVEP D N+MA
Sbjct: 242 IAGQGTVGMEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMA 301

Query: 326 QALKRGYIVNVTDIGHFADGVAVQQVGNETFRICNEVVDDIILVDKYAISKAVQDMFNDG 385
            +L  G  V +  +G FADGVAV++VG ETFRI   ++D ++LV + AI  +++DMF + 
Sbjct: 302 LSLHHGERVILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEK 361

Query: 386 RNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGANMNFDQLRVISDIAN--NDTQTIL 445
           RNILEP+GAL++A A+AYC+Y  +K VNVVA+TSGANMNFD+LR+++++AN     + +L
Sbjct: 362 RNILEPAGALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVL 421

Query: 446 VSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGSTDAVVVYKVDA-VGSDLENLLLDL 505
            +++PE PGS  +  +L+     NI E  YR      +AVV+Y V      +L+ L   +
Sbjct: 422 ATLMPEKPGSFKQFCELVGP--MNISEFKYRC-SSEKEAVVLYSVGVHTAGELKALQKRM 481

Query: 506 NSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRFSFPERKGALKHFLHGFQPNWNI 565
            S   +T  L  +D    HLRY+ GGR+ V +E L RF+FPER GAL +FL  F P WNI
Sbjct: 482 ESSQLKTVNLTTSDLVKDHLRYLMGGRSTVGDEVLCRFTFPERPGALMNFLDSFSPRWNI 541

Query: 566 SLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGHEGLMPKVDNIISL 611
           +LFH+R +G   ++VLVGIQ+ + E + F   AK +G++  +   D+   L
Sbjct: 542 TLFHYRGQGETGANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDDYFKL 589

BLAST of Spg012737 vs. ExPASy Swiss-Prot
Match: Q9AXU4 (Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 2.2e-144
Identity = 265/496 (53.43%), Postives = 351/496 (70.77%), Query Frame = 0

Query: 105 PTEVDKEKQMGYITKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIR 164
           P+  D E+   Y+ +IL S VYDVA+++PLQ A  LS+KLGVN W+KRED Q VFSFK+R
Sbjct: 93  PSGGDTEELFQYLVEILASRVYDVAIDSPLQNAAKLSKKLGVNFWIKREDMQSVFSFKLR 152

Query: 165 GAYNMISQLPEDQRKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRL 224
           GAYNM+++L ++Q ++GVI ASAGNHAQGVAL A+RLK  A IVMP TTP+IKI+AVK L
Sbjct: 153 GAYNMMTKLSKEQLERGVITASAGNHAQGVALGAQRLKCTATIVMPVTTPEIKIEAVKNL 212

Query: 225 GGAVETWGDTFDKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAM 284
            G V   GDTFDKAQE A + A DE LTFIPPFD   VI GQGT+G EI+RQ+K +IHA+
Sbjct: 213 DGKVVLHGDTFDKAQEHALKLAEDEGLTFIPPFDHPDVIIGQGTIGTEINRQLK-DIHAV 272

Query: 285 FVPVGGGGLISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVA 344
           FVPVGGGGLI+G+A+++K V P  KI GVEP   +SM Q+L  G  V +  + +FADGVA
Sbjct: 273 FVPVGGGGLIAGVAAYFKRVAPHTKIIGVEPFGASSMTQSLYHGERVKLEQVDNFADGVA 332

Query: 345 VQQVGNETFRICNEVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYN 404
           V  VG ETFR+C +++D ++LV   AIS AV+D++++GRNILE SGAL+IA A+AYC+Y 
Sbjct: 333 VALVGEETFRLCKDLIDGMVLVSNDAISAAVKDVYDEGRNILETSGALAIAGAEAYCKYY 392

Query: 405 NIKGVNVVAVTSGANMNFDQLRVISDIAN--NDTQTILVSILPEVPGSLDKLTKLIDEKG 464
           NIKG NVVA+ SGANM+F +L+++ D+A+     + +L + +PE PGS  K  +L+    
Sbjct: 393 NIKGENVVAIASGANMDFSKLKLVVDLADIGGQREALLATFMPEEPGSFKKFCELVGP-- 452

Query: 465 FNILETSYRFGEGSTDAVVVYKVDA-VGSDLENLLLDLNSFGYETYALNDNDAAASHLRY 524
            NI E  YR+  G   A+V+Y V     SDLE++L  + S    T  L +N+    HLR+
Sbjct: 453 MNITEFKYRYNSGRKQALVLYSVGVNTKSDLESMLERMKSSQLNTVNLTNNNLVKEHLRH 512

Query: 525 MTGGRAKVENETLFRFSFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLE 584
           + GGR++  NE   +F FPE+ GAL+ FL  F P WNISLFH+R +G L +SVLVG Q+ 
Sbjct: 513 LMGGRSEPSNEIFCQFIFPEKPGALRKFLDAFSPRWNISLFHYREQGELDASVLVGFQVP 572

Query: 585 KSEEKNFHAYAKKVGH 598
           K E + F   A  +G+
Sbjct: 573 KGEIEEFRVQANNLGY 585

BLAST of Spg012737 vs. ExPASy Swiss-Prot
Match: P25306 (Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD2 PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 6.4e-128
Identity = 241/517 (46.62%), Postives = 341/517 (65.96%), Query Frame = 0

Query: 104 KPTEVDKEKQMGYITKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKI 163
           KPT  D ++   Y+  IL S VYDVA+E+PL+LA  LS +LGVN ++KRED Q+VFSFK+
Sbjct: 85  KPTGGDSDELFQYLVDILASPVYDVAIESPLELAEKLSDRLGVNFYIKREDKQRVFSFKL 144

Query: 164 RGAYNMISQLPEDQRKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKR 223
           RGAYNM+S L  ++  KGVI ASAGNHAQGVAL+ +RL  VA IVMPTTTPQIKIDAV+ 
Sbjct: 145 RGAYNMMSNLSREELDKGVITASAGNHAQGVALAGQRLNCVAKIVMPTTTPQIKIDAVRA 204

Query: 224 LGGAVETWGDTFDKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHA 283
           LGG V  +G TFD+AQ  A + +  + L +IPPFDD  VI GQGT+G EI+RQ+K +IHA
Sbjct: 205 LGGDVVLYGKTFDEAQTHALELSEKDGLKYIPPFDDPGVIKGQGTIGTEINRQLK-DIHA 264

Query: 284 MFVPVGGGGLISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGV 343
           +F+PVGGGGLI+G+A+F+K + P  KI GVEP    SM  +L  G+ V ++++  FADGV
Sbjct: 265 VFIPVGGGGLIAGVATFFKQIAPNTKIIGVEPYGAASMTLSLHEGHRVKLSNVDTFADGV 324

Query: 344 AVQQVGNETFRICNEVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEY 403
           AV  VG  TF  C E++D ++LV    IS A++D++++GRNILE SGA++IA A AYCE+
Sbjct: 325 AVALVGEYTFAKCQELIDGMVLVANDGISAAIKDVYDEGRNILETSGAVAIAGAAAYCEF 384

Query: 404 NNIKGVNVVAVTSGANMNFDQLRVISDIA--NNDTQTILVSILPEVPGSLDKLTKLIDEK 463
             IK  N+VA+ SGANM+F +L  ++++A   +  + +L + + E  GS      L+   
Sbjct: 385 YKIKNENIVAIASGANMDFSKLHKVTELAGLGSGKEALLATFMVEQQGSFKTFVGLVG-- 444

Query: 464 GFNILETSYRFGEGSTDAVVVYKVDA-VGSDLENLLLDLNSFGYETYALNDNDAAASHLR 523
             N  E +YRF     +A+++Y+V+    SDLE ++ D+ S    T  L+ N+    HL+
Sbjct: 445 SLNFTELTYRFTSERKNALILYRVNVDKESDLEKMIEDMKSSNMTTLNLSHNELVVDHLK 504

Query: 524 YMTGGRAKVENETLFRFSFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQL 583
           ++ GG A + +E    F  PE+   LK FL  F P WNI+L  +R +G + +S+L+G Q+
Sbjct: 505 HLVGGSANISDEIFGEFIVPEKAETLKTFLDAFSPRWNITLCRYRNQGDINASLLMGFQV 564

Query: 584 EKSEEKNFHAYAKKVGHEGLMPKVDNIISLWRYVVVE 618
            ++E   F   A K+G+     ++DN    +  VV E
Sbjct: 565 PQAEMDEFKNQADKLGYP---YELDNYNEAFNLVVSE 595

BLAST of Spg012737 vs. ExPASy Swiss-Prot
Match: P53607 (L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=ilvA PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 2.5e-116
Identity = 219/488 (44.88%), Postives = 318/488 (65.16%), Query Frame = 0

Query: 116 YITKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPE 175
           Y+ KIL + VYDVA ET L+ A  LS +L   ++LKRED+Q VFSFK+RGAYN ++ +P 
Sbjct: 6   YLKKILTARVYDVAFETELEPARNLSARLRNPVYLKREDNQPVFSFKLRGAYNKMAHIPA 65

Query: 176 DQRKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGG---AVETWG 235
           D   +GVI ASAGNHAQGVA SA R+ + A IV+P TTPQ+K+DAV+  GG    V   G
Sbjct: 66  DALARGVITASAGNHAQGVAFSAARMGVKAVIVVPVTTPQVKVDAVRAHGGPGVEVIQAG 125

Query: 236 DTFDKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGG 295
           +++  A   A +   +  LTF+ PFDD +VIAGQGT+ MEI RQ +G IHA+FVP+GGGG
Sbjct: 126 ESYSDAYAHALKVQEERGLTFVHPFDDPYVIAGQGTIAMEILRQHQGPIHAIFVPIGGGG 185

Query: 296 LISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNET 355
           L +G+A++ K V P +K+ GV+  D  +MAQ+L+ G  V + ++G FADG AV+ VG ET
Sbjct: 186 LAAGVAAYVKAVRPEIKVIGVQAEDSCAMAQSLQAGKRVELAEVGLFADGTAVKLVGEET 245

Query: 356 FRICNEVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVV 415
           FR+C E +D ++ VD  A+  A++D+F D R++LEPSGAL++A AK Y E   I+   +V
Sbjct: 246 FRLCKEYLDGVVTVDTDALCAAIKDVFQDTRSVLEPSGALAVAGAKLYAEREGIENQTLV 305

Query: 416 AVTSGANMNFDQLRVISDIA--NNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSY 475
           AVTSGANMNFD++R +++ A      + +    +PE  GS  +   L+ ++  N+ E +Y
Sbjct: 306 AVTSGANMNFDRMRFVAERAEVGEAREAVFAVTIPEERGSFKRFCSLVGDR--NVTEFNY 365

Query: 476 RFGEGSTDAVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVE 535
           R  +  +  + V        +  ++  +  S G++T  L  ++ +  H+RYM GGR+ + 
Sbjct: 366 RIADAQSAHIFVGVQIRRRGESADIAANFESHGFKTADLTHDELSKEHIRYMVGGRSPLA 425

Query: 536 -NETLFRFSFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFH 595
            +E LFRF FPER GAL  FL    P+WNISLFH+R +G   SS+LVG+Q+ +++   F 
Sbjct: 426 LDERLFRFEFPERPGALMKFLSSMAPDWNISLFHYRNQGADYSSILVGLQVPQADHAEFE 485

Query: 596 AYAKKVGH 598
            +   +G+
Sbjct: 486 RFLAALGY 491

BLAST of Spg012737 vs. ExPASy TrEMBL
Match: A0A6J1ILR7 (Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477609 PE=3 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 9.3e-239
Identity = 436/600 (72.67%), Postives = 499/600 (83.17%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSK 60
           MES+LLT TTSN+   RNS +SSLS SSSL +  S KVE+RRS  G  K P  + +S   
Sbjct: 1   MESILLTATTSNIPLPRNSSLSSLSFSSSLPNQVSTKVEIRRSNKGTTKAPRIVASSSGS 60

Query: 61  RG-TGKG-TANVAVDGSVAAVAADVPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYIT 120
           +G  GKG +ANVAVDGSV  VA  V DVSW E+QY  GS GHR P PT+ D EKQM YIT
Sbjct: 61  KGRAGKGVSANVAVDGSV--VAEGVKDVSWKELQYEEGSTGHRLPLPTDPDTEKQMEYIT 120

Query: 121 KILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQR 180
            ILGS VYDVA+ETPLQLAPLLS +LGVNLWLKREDSQQVFSFKIRGAYNMIS+LPE Q 
Sbjct: 121 NILGSNVYDVAIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISKLPEKQL 180

Query: 181 KKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDKA 240
           KKG+ICASAGNHAQGVALS +RLK  A IVMPTTTPQIKIDAV+RLGG V+ +G++FD+A
Sbjct: 181 KKGIICASAGNHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLYGNSFDEA 240

Query: 241 QERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGIA 300
           Q+RAK+R+ +E LTFIPPFDD+ VIAGQGTVGMEI RQ++G IHA+FVP+GGGGL SGI 
Sbjct: 241 QKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIV 300

Query: 301 SFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICNE 360
           SFYKLVYP VK+FGVEPND+NSMAQAL RG IVNVTDIGHFADGVAVQQVG+ETFRIC E
Sbjct: 301 SFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGDETFRICYE 360

Query: 361 VVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA 420
           ++DD+ILV K +IS A++DMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA
Sbjct: 361 LLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA 420

Query: 421 NMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGSTD 480
           NMNFDQL  ISD+AN D Q+IL ++LPE PGSL +LT LI + G  I E +YRF  GSTD
Sbjct: 421 NMNFDQLGQISDLANID-QSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGSTD 480

Query: 481 AVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRFS 540
           A+VVYKV AV S LE L+  LN FG++TY L+DN+   +HLR+M GGR  VENETLFRF+
Sbjct: 481 ALVVYKVKAVDSALETLVRQLNFFGFKTYTLSDNEVVRNHLRFMMGGRQNVENETLFRFT 540

Query: 541 FPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGHE 599
           FPE+ GALKHFL  FQPNWN+SLFHHR +GIL S VL+G+QLEKSEEK FH YAKKVG++
Sbjct: 541 FPEKVGALKHFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFHEYAKKVGYQ 597

BLAST of Spg012737 vs. ExPASy TrEMBL
Match: A0A6J1IJC6 (Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477609 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 4.8e-235
Identity = 433/600 (72.17%), Postives = 494/600 (82.33%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSK 60
           MES+LLT TTSN+   RNS +SSLS SSSL +  S KVE+RRS  G  K P  + +S   
Sbjct: 1   MESILLTATTSNIPLPRNSSLSSLSFSSSLPNQVSTKVEIRRSNKGTTKAPRIVASSSGS 60

Query: 61  RG-TGKG-TANVAVDGSVAAVAADVPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYIT 120
           +G  GKG +ANVAVDGSV  VA  V DVSW E+QY  GS GHR P PT+ D EKQM YIT
Sbjct: 61  KGRAGKGVSANVAVDGSV--VAEGVKDVSWKELQYEEGSTGHRLPLPTDPDTEKQMEYIT 120

Query: 121 KILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQR 180
            ILGS VYDVA+ETPLQLAPLLS +LGVNLWLKREDSQQVFSFKIRGAYNMIS+LPE Q 
Sbjct: 121 NILGSNVYDVAIETPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISKLPEKQL 180

Query: 181 KKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDKA 240
           KKG+ICASAGNHAQGVALS +RLK  A IVMPTTTPQIKIDAV+RLGG V+ +G++FD+A
Sbjct: 181 KKGIICASAGNHAQGVALSGQRLKTKAYIVMPTTTPQIKIDAVERLGGIVDLYGNSFDEA 240

Query: 241 QERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGIA 300
           Q+RAK+R+ +E LTFIPPFDD+ VIAGQGTVGMEI RQ++G IHA+FVP+GGGGL SGI 
Sbjct: 241 QKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGIV 300

Query: 301 SFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICNE 360
           SFYKLVYP VK+FGVEPND+NSMAQAL RG IVNVTDIGHFADGVAVQQVG+ETFRIC E
Sbjct: 301 SFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGDETFRICYE 360

Query: 361 VVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA 420
           ++DD+ILV K +IS A++DMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA
Sbjct: 361 LLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGA 420

Query: 421 NMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGSTD 480
           NMNFDQL  ISD+AN D Q+IL ++LPE PGSL +LT LI + G  I E +YRF  GSTD
Sbjct: 421 NMNFDQLGQISDLANID-QSILATMLPETPGSLKQLTDLIADLGVTITEMTYRFSSGSTD 480

Query: 481 AVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRFS 540
           A+VVYKV AV S LE L+  LN FG++TY L+DN+   +HLR+M GGR  VENETLFRF+
Sbjct: 481 ALVVYKVKAVDSALETLVRQLNFFGFKTYTLSDNEVVRNHLRFMMGGRQNVENETLFRFT 540

Query: 541 FPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGHE 599
           FPE+ GAL HFL GFQPNWNISL HH   GI+ S VL+GIQLEKS EK F  YAKKVG++
Sbjct: 541 FPEKAGALNHFLKGFQPNWNISLLHHPDLGIITSDVLIGIQLEKSLEKKFDEYAKKVGYQ 597

BLAST of Spg012737 vs. ExPASy TrEMBL
Match: A0A6J1ECR5 (Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433136 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 1.2e-233
Identity = 425/601 (70.72%), Postives = 496/601 (82.53%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSLSSSSSLGSIPSGKVEVRRSRIGKVKTPTSIVASVSK 60
           MES+LLT TTSNL   RNS +S LS SSSL +  S KV++RR+  G  K P  + +S   
Sbjct: 1   MESILLTATTSNLPLPRNSSLSPLSFSSSLPNQVSTKVDIRRTNKGTTKAPRIVASSSGS 60

Query: 61  RG-TGKG-TANVAVDGSVAAVAAD-VPDVSWTEVQYPAGSIGHRHPKPTEVDKEKQMGYI 120
           +G  GKG +ANVAVDGSVAAV A+ V DVSW E+QY  GS GHR P P + D EKQM YI
Sbjct: 61  KGRAGKGVSANVAVDGSVAAVGAEAVKDVSWKELQYEEGSTGHRQPLPADPDTEKQMEYI 120

Query: 121 TKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQ 180
           T ILGS VYDVA+E+PLQLAPLLS +LGVNLWLKREDSQQVFSFKIRGAYNMIS+L E++
Sbjct: 121 TNILGSNVYDVAIESPLQLAPLLSTQLGVNLWLKREDSQQVFSFKIRGAYNMISRLSEEK 180

Query: 181 RKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDK 240
            KKG+ICASAGNHAQGVAL+ +RLK  A IVMPTTTPQIKIDAV+RL G V+ +G++FD+
Sbjct: 181 LKKGIICASAGNHAQGVALAGQRLKTKAYIVMPTTTPQIKIDAVERLDGIVDLYGNSFDE 240

Query: 241 AQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGI 300
           AQ+RAK+R+ +E LTFIPPFDD+ VIAGQGTVGMEI RQ++G IHA+FVP+GGGGL SGI
Sbjct: 241 AQKRAKERSEEEGLTFIPPFDDKDVIAGQGTVGMEIGRQIRGKIHAIFVPIGGGGLASGI 300

Query: 301 ASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICN 360
            SFYKLVYP VK+FGVEPND+NSMAQAL RG IVNVTDIGHFADGVAVQQVG ETFRIC 
Sbjct: 301 VSFYKLVYPDVKVFGVEPNDQNSMAQALYRGEIVNVTDIGHFADGVAVQQVGEETFRICY 360

Query: 361 EVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSG 420
           E++DD+ILV K +IS A++DMFNDGRNILEPSGALSIA AKAYCEYNNIKGVN+VAVTSG
Sbjct: 361 ELLDDVILVKKESISAAIKDMFNDGRNILEPSGALSIAGAKAYCEYNNIKGVNIVAVTSG 420

Query: 421 ANMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGST 480
           ANMNFDQL  ISD+AN D Q+IL ++LPE PGSL +LT LI + G  I E +YRF  GST
Sbjct: 421 ANMNFDQLGQISDLANID-QSILATMLPETPGSLKQLTDLIADLGVTIAEMTYRFSSGST 480

Query: 481 DAVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRF 540
           DA+VVYKV AV + LE L+  L+ FG++TY L+DN+   +HLRYM GGR  VENETLFRF
Sbjct: 481 DALVVYKVKAVDAALETLVRQLDFFGFKTYTLSDNEVVRNHLRYMMGGRQNVENETLFRF 540

Query: 541 SFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGH 599
           +FPE+ GALKHFL  FQPNWN+SLFHHR +GIL S VL+G+QLEKSEEK F  YAK+VG+
Sbjct: 541 TFPEKVGALKHFLKDFQPNWNVSLFHHRHQGILTSDVLIGVQLEKSEEKKFDEYAKRVGY 600

BLAST of Spg012737 vs. ExPASy TrEMBL
Match: A0A5D3C7Q3 (Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00790 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 3.0e-229
Identity = 431/606 (71.12%), Postives = 501/606 (82.67%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSL-SSSSSLGSIPSGKVEVRRSRIGKVKT-----PTSI 60
           MES LLTT   N L GRNS +SSL SSSSS+ +  + KVE+ R RI KV T     PTSI
Sbjct: 1   MESFLLTT---NPLLGRNSSLSSLPSSSSSISTQLNSKVEIIRRRIEKVNTTTTHVPTSI 60

Query: 61  VA-SVSKRGTGKG-TANVAVDGSVAAVAADVPDVSWTEVQYPAGSIGHRHPKPTEVDKEK 120
           VA S SKR TGKG +ANVAVDGSVA V   V DVSWTEVQY +GSIGHR P P EVDK+K
Sbjct: 61  VASSASKRKTGKGLSANVAVDGSVATVGT-VKDVSWTEVQYESGSIGHRAPPPAEVDKDK 120

Query: 121 QMGYITKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQ 180
           QM YITKILGS VYDVAVETPL+LAPLLS ++G+NL+LKRED+QQVFSFKIRGAYNMISQ
Sbjct: 121 QMEYITKILGSKVYDVAVETPLELAPLLSTQIGINLYLKREDAQQVFSFKIRGAYNMISQ 180

Query: 181 LPEDQRKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWG 240
           LP+DQ   GVICASAGNHAQGVALSA+RLKI A IVMPTTTP IK++AVKRLGG V   G
Sbjct: 181 LPKDQLANGVICASAGNHAQGVALSAQRLKIKAQIVMPTTTPAIKVEAVKRLGGKVIQEG 240

Query: 241 DTFDKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGG 300
           +TFD++Q+ AK+ +    +TFIPPFD+E VIAGQGTVGMEI RQM+G IHA+ VP+GGGG
Sbjct: 241 ETFDESQKIAKEISSRLGITFIPPFDNEDVIAGQGTVGMEIGRQMRGKIHAIIVPIGGGG 300

Query: 301 LISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNET 360
           L +GI SFYKLVYP VK+FGVEPNDENSMAQAL R  IVNVTDIGHFADGVAVQQVG+ET
Sbjct: 301 LAAGIVSFYKLVYPEVKVFGVEPNDENSMAQALYRDEIVNVTDIGHFADGVAVQQVGDET 360

Query: 361 FRICNEVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVV 420
           FRIC E++DD+ILV K  IS A++DMF+D R+ILEPSGALSIAAAKAYC+YNNI GVN+V
Sbjct: 361 FRICRELLDDVILVTKEEISAAIKDMFSDERSILEPSGALSIAAAKAYCKYNNITGVNIV 420

Query: 421 AVTSGANMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRF 480
           AV SGANMNFDQLR ISDIAN D Q+I+ +ILPE PGSL +LT ++DE   +I E +YRF
Sbjct: 421 AVCSGANMNFDQLREISDIANVD-QSIICTILPETPGSLQQLTDMLDE---DITEMTYRF 480

Query: 481 GEGSTDAVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENE 540
             GS DAVVVYKV AVG++LE+++  LN+ G+ TY LNDN    +HLRYMTGGRA +ENE
Sbjct: 481 SSGSQDAVVVYKVSAVGTELEDMVEMLNTAGFTTYTLNDNPVVKNHLRYMTGGRADLENE 540

Query: 541 TLFRFSFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYA 599
           TLFRF+FPERKGALKHF   F+ +WN+SLFHHRA+GIL S VL+G+QLEKSEEK+FH + 
Sbjct: 541 TLFRFTFPERKGALKHFFKDFKQSWNVSLFHHRAQGILTSDVLIGVQLEKSEEKDFHEHL 598

BLAST of Spg012737 vs. ExPASy TrEMBL
Match: A0A1S3CH38 (Threonine dehydratase OS=Cucumis melo OX=3656 GN=LOC103500853 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 3.0e-229
Identity = 431/606 (71.12%), Postives = 501/606 (82.67%), Query Frame = 0

Query: 1   MESLLLTTTTSNLLTGRNSPVSSL-SSSSSLGSIPSGKVEVRRSRIGKVKT-----PTSI 60
           MES LLTT   N L GRNS +SSL SSSSS+ +  + KVE+ R RI KV T     PTSI
Sbjct: 1   MESFLLTT---NPLLGRNSSLSSLPSSSSSISTQLNSKVEIIRRRIEKVNTTTTHVPTSI 60

Query: 61  VA-SVSKRGTGKG-TANVAVDGSVAAVAADVPDVSWTEVQYPAGSIGHRHPKPTEVDKEK 120
           VA S SKR TGKG +ANVAVDGSVA V   V DVSWTEVQY +GSIGHR P P EVDK+K
Sbjct: 61  VASSASKRKTGKGLSANVAVDGSVATVGT-VKDVSWTEVQYESGSIGHRAPPPAEVDKDK 120

Query: 121 QMGYITKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQ 180
           QM YITKILGS VYDVAVETPL+LAPLLS ++G+NL+LKRED+QQVFSFKIRGAYNMISQ
Sbjct: 121 QMEYITKILGSKVYDVAVETPLELAPLLSTQIGINLYLKREDAQQVFSFKIRGAYNMISQ 180

Query: 181 LPEDQRKKGVICASAGNHAQGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWG 240
           LP+DQ   GVICASAGNHAQGVALSA+RLKI A IVMPTTTP IK++AVKRLGG V   G
Sbjct: 181 LPKDQLANGVICASAGNHAQGVALSAQRLKIKAQIVMPTTTPAIKVEAVKRLGGKVIQEG 240

Query: 241 DTFDKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGG 300
           +TFD++Q+ AK+ +    +TFIPPFD+E VIAGQGTVGMEI RQM+G IHA+ VP+GGGG
Sbjct: 241 ETFDESQKIAKEISSRLGITFIPPFDNEDVIAGQGTVGMEIGRQMRGKIHAIIVPIGGGG 300

Query: 301 LISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNET 360
           L +GI SFYKLVYP VK+FGVEPNDENSMAQAL R  IVNVTDIGHFADGVAVQQVG+ET
Sbjct: 301 LAAGIVSFYKLVYPEVKVFGVEPNDENSMAQALYRDEIVNVTDIGHFADGVAVQQVGDET 360

Query: 361 FRICNEVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVV 420
           FRIC E++DD+ILV K  IS A++DMF+D R+ILEPSGALSIAAAKAYC+YNNI GVN+V
Sbjct: 361 FRICRELLDDVILVTKEEISAAIKDMFSDERSILEPSGALSIAAAKAYCKYNNITGVNIV 420

Query: 421 AVTSGANMNFDQLRVISDIANNDTQTILVSILPEVPGSLDKLTKLIDEKGFNILETSYRF 480
           AV SGANMNFDQLR ISDIAN D Q+I+ +ILPE PGSL +LT ++DE   +I E +YRF
Sbjct: 421 AVCSGANMNFDQLREISDIANVD-QSIICTILPETPGSLQQLTDMLDE---DITEMTYRF 480

Query: 481 GEGSTDAVVVYKVDAVGSDLENLLLDLNSFGYETYALNDNDAAASHLRYMTGGRAKVENE 540
             GS DAVVVYKV AVG++LE+++  LN+ G+ TY LNDN    +HLRYMTGGRA +ENE
Sbjct: 481 SSGSQDAVVVYKVSAVGTELEDMVEMLNTAGFTTYTLNDNPVVKNHLRYMTGGRADLENE 540

Query: 541 TLFRFSFPERKGALKHFLHGFQPNWNISLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYA 599
           TLFRF+FPERKGALKHF   F+ +WN+SLFHHRA+GIL S VL+G+QLEKSEEK+FH + 
Sbjct: 541 TLFRFTFPERKGALKHFFKDFKQSWNVSLFHHRAQGILTSDVLIGVQLEKSEEKDFHEHL 598

BLAST of Spg012737 vs. TAIR 10
Match: AT3G10050.1 (L-O-methylthreonine resistant 1 )

HSP 1 Score: 518.5 bits (1334), Expect = 8.3e-147
Identity = 274/531 (51.60%), Postives = 367/531 (69.11%), Query Frame = 0

Query: 86  VSWTEVQYPAGSIGHRHPKPTEVDK---EKQMGYITKILGSTVYDVAVETPLQLAPLLSQ 145
           VS   +QYPAG +G    +  E +     + M Y+T IL + VYD+A+E+PLQLA  LS+
Sbjct: 62  VSPNSLQYPAGYLGAVPERTNEAENGSIAEAMEYLTNILSTKVYDIAIESPLQLAKKLSK 121

Query: 146 KLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQRKKGVICASAGNHAQGVALSAKRLK 205
           +LGV ++LKRED Q VFSFK+RGAYNM+ +LP DQ  KGVIC+SAGNHAQGVALSA +L 
Sbjct: 122 RLGVRMYLKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLG 181

Query: 206 IVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDKAQERAKQRAIDEDLTFIPPFDDEHV 265
             A IVMP TTP+IK  AV+ LG  V  +GD++D+AQ  AK RA +E LTFIPPFD   V
Sbjct: 182 CTAVIVMPVTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDV 241

Query: 266 IAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGIASFYKLVYPGVKIFGVEPNDENSMA 325
           IAGQGTVGMEI+RQ KG +HA+FVPVGGGGLI+GIA++ K V P VKI GVEP D N+MA
Sbjct: 242 IAGQGTVGMEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMA 301

Query: 326 QALKRGYIVNVTDIGHFADGVAVQQVGNETFRICNEVVDDIILVDKYAISKAVQDMFNDG 385
            +L  G  V +  +G FADGVAV++VG ETFRI   ++D ++LV + AI  +++DMF + 
Sbjct: 302 LSLHHGERVILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEK 361

Query: 386 RNILEPSGALSIAAAKAYCEYNNIKGVNVVAVTSGANMNFDQLRVISDIAN--NDTQTIL 445
           RNILEP+GAL++A A+AYC+Y  +K VNVVA+TSGANMNFD+LR+++++AN     + +L
Sbjct: 362 RNILEPAGALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVL 421

Query: 446 VSILPEVPGSLDKLTKLIDEKGFNILETSYRFGEGSTDAVVVYKVDA-VGSDLENLLLDL 505
            +++PE PGS  +  +L+     NI E  YR      +AVV+Y V      +L+ L   +
Sbjct: 422 ATLMPEKPGSFKQFCELVGP--MNISEFKYRC-SSEKEAVVLYSVGVHTAGELKALQKRM 481

Query: 506 NSFGYETYALNDNDAAASHLRYMTGGRAKVENETLFRFSFPERKGALKHFLHGFQPNWNI 565
            S   +T  L  +D    HLRY+ GGR+ V +E L RF+FPER GAL +FL  F P WNI
Sbjct: 482 ESSQLKTVNLTTSDLVKDHLRYLMGGRSTVGDEVLCRFTFPERPGALMNFLDSFSPRWNI 541

Query: 566 SLFHHRAEGILKSSVLVGIQLEKSEEKNFHAYAKKVGHEGLMPKVDNIISL 611
           +LFH+R +G   ++VLVGIQ+ + E + F   AK +G++  +   D+   L
Sbjct: 542 TLFHYRGQGETGANVLVGIQVPEQEMEEFKNRAKALGYDYFLVSDDDYFKL 589

BLAST of Spg012737 vs. TAIR 10
Match: AT4G11640.1 (serine racemase )

HSP 1 Score: 159.5 bits (402), Expect = 9.8e-39
Identity = 99/298 (33.22%), Postives = 164/298 (55.03%), Query Frame = 0

Query: 132 TPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQRKKGVICASAGNHA 191
           TP+  +  L+   G +L+ K E  Q+  +FK RGA N +  L  +Q  KGV+  S+GNHA
Sbjct: 29  TPVLTSESLNSISGRSLFFKCECLQKGGAFKFRGACNAVLSLDAEQAAKGVVTHSSGNHA 88

Query: 192 QGVALSAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVETWGDTFDKAQERAKQRAIDE-D 251
             ++L+AK   I A IV+P   P+ K+D V R GG V  W +    ++E    + + E  
Sbjct: 89  AALSLAAKIQGIPAYIVVPKGAPKCKVDNVIRYGGKV-IWSEATMSSREEIASKVLQETG 148

Query: 252 LTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGLISGIASFYKLVYPGVKI 311
              I P++D  +I+GQGT+ +E+  Q++  I A+ VP+ GGGLISG+A   K + P ++I
Sbjct: 149 SVLIHPYNDGRIISGQGTIALELLEQIQ-EIDAIVVPISGGGLISGVALAAKSIKPSIRI 208

Query: 312 FGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETFRICNEVVDDIILVDKYA 371
              EP   +  AQ+   G I+ +      ADG+    +G+ T+ +  ++VDD++ +++  
Sbjct: 209 IAAEPKGADDAAQSKVAGKIITLPVTNTIADGLRA-SLGDLTWPVVRDLVDDVVTLEECE 268

Query: 372 ISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIK---GVNVVAVTSGANMNFDQL 426
           I +A++  +   +  +EPSGA+ +AA  +    NN       N+  V SG N++   L
Sbjct: 269 IIEAMKMCYEILKVSVEPSGAIGLAAVLSNSFRNNPSCRDCKNIGIVLSGGNVDLGSL 323

BLAST of Spg012737 vs. TAIR 10
Match: AT3G61440.1 (cysteine synthase C1 )

HSP 1 Score: 55.1 bits (131), Expect = 2.6e-07
Identity = 76/320 (23.75%), Postives = 127/320 (39.69%), Query Frame = 0

Query: 131 ETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPEDQ----RKKGVICAS 190
           +TPL     +++     +  K+E  Q   S K R A  MI+   + +     K  +I  +
Sbjct: 59  KTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTLIEPT 118

Query: 191 AGNHAQGVALSAKRLKIVANIVMPTTT-------------PQIKIDAVKRLGGAVETWGD 250
           +GN    +A  A        + MP+ T               +  D  K +GG V+   D
Sbjct: 119 SGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKKAYD 178

Query: 251 TFDKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMKGNIHAMFVPVGGGGL 310
             D   +    +           FD         T G EI     GN+    + +G GG 
Sbjct: 179 LLDSTPDAFMCQQFANPANTQIHFD---------TTGPEIWEDTLGNVDIFVMGIGSGGT 238

Query: 311 ISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQQVGNETF 370
           +SG+  + K   P VKI+GVEP + N          I+N    G  A  +    VG +  
Sbjct: 239 VSGVGRYLKSKNPNVKIYGVEPAESN----------ILNGGKPGPHA--ITGNGVGFKPE 298

Query: 371 RICNEVVDDIILV-DKYAISKAVQDMFNDGRNILEPSGALSIAAAKAYCEYNNIKGVNVV 427
            +  +V++ ++ V  + AI  A +    +G  +   SGA ++AA +      N KG  +V
Sbjct: 299 ILDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSGANTVAAIRLAKMPEN-KGKLIV 356

BLAST of Spg012737 vs. TAIR 10
Match: AT3G03630.1 (cysteine synthase 26 )

HSP 1 Score: 53.9 bits (128), Expect = 5.8e-07
Identity = 64/290 (22.07%), Postives = 121/290 (41.72%), Query Frame = 0

Query: 117 ITKILGSTVYDVAVETPLQLAPLLSQKLGVNLWLKREDSQQVFSFKIRGAYNMISQLPED 176
           +T+++GS        TP+     ++     ++  K E  +   S K R   +MI++    
Sbjct: 103 VTQLIGS--------TPMVYLNRVTDGCLADIAAKLESMEPCRSVKDRIGLSMINEAENS 162

Query: 177 ----QRKKGVICASAGNHAQGVAL--SAKRLKIVANIVMPTTTPQIKIDAVKRLGGAVET 236
                RK  ++  + GN   G+A   +AK  K++  + MP +    +   ++ LG  +  
Sbjct: 163 GAITPRKTVLVEPTTGNTGLGIAFVAAAKGYKLI--VTMPASINIERRMLLRALGAEIVL 222

Query: 237 WGDT--FDKAQERAKQRAI-DEDLTFIPPFDD-EHVIAGQGTVGMEISRQMKGNIHAMFV 296
                    A ++AK+  +  ++      FD+  +      T G EI     GN+     
Sbjct: 223 TNPEKGLKGAVDKAKEIVLKTKNAYMFQQFDNTANTKIHFETTGPEIWEDTMGNVDIFVA 282

Query: 297 PVGGGGLISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGHFADGVAVQ 356
            +G GG ++G   F K++   +K+ GVEP+ E S+      GY+  + D+          
Sbjct: 283 GIGTGGTVTGTGGFLKMMNKDIKVVGVEPS-ERSVISGDNPGYLPGILDV---------- 342

Query: 357 QVGNETFRICNEVVDDIILVDKYAISKAVQDMFNDGRNILEPSGALSIAA 397
           ++ +E F++ N            AI  A +    +G  +   SGA ++AA
Sbjct: 343 KLLDEVFKVSN----------GEAIEMARRLALEEGLLVGISSGAAAVAA 361

BLAST of Spg012737 vs. TAIR 10
Match: AT3G61440.2 (cysteine synthase C1 )

HSP 1 Score: 50.8 bits (120), Expect = 4.9e-06
Identity = 55/215 (25.58%), Postives = 90/215 (41.86%), Query Frame = 0

Query: 219 DAVKRLGGAVETWGDTFDKAQERAKQRAIDEDLTFIPPFDDEHVIAGQGTVGMEISRQMK 278
           D  K +GG V+   D  D   +    +           FD         T G EI     
Sbjct: 68  DPAKGMGGTVKKAYDLLDSTPDAFMCQQFANPANTQIHFD---------TTGPEIWEDTL 127

Query: 279 GNIHAMFVPVGGGGLISGIASFYKLVYPGVKIFGVEPNDENSMAQALKRGYIVNVTDIGH 338
           GN+    + +G GG +SG+  + K   P VKI+GVEP + N          I+N    G 
Sbjct: 128 GNVDIFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESN----------ILNGGKPGP 187

Query: 339 FADGVAVQQVGNETFRICNEVVDDIILV-DKYAISKAVQDMFNDGRNILEPSGALSIAAA 398
            A  +    VG +   +  +V++ ++ V  + AI  A +    +G  +   SGA ++AA 
Sbjct: 188 HA--ITGNGVGFKPEILDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSGANTVAAI 247

Query: 399 KAYCEYNNIKGVNVVAVTSG------ANMNFDQLR 427
           +      N KG  +V + +       +++ FD+LR
Sbjct: 248 RLAKMPEN-KGKLIVTIHASFGERYLSSVLFDELR 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6604380.12.7e-24073.04Threonine dehydratase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7034531.11.0e-23973.04Threonine dehydratase biosynthetic, chloroplastic [Cucurbita argyrosperma subsp.... [more]
XP_023543427.12.3e-23972.88threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita pepo subsp. pe... [more]
XP_022977240.11.9e-23872.67threonine dehydratase biosynthetic, chloroplastic-like isoform X1 [Cucurbita max... [more]
XP_022977241.19.9e-23572.17threonine dehydratase biosynthetic, chloroplastic-like isoform X2 [Cucurbita max... [more]
Match NameE-valueIdentityDescription
A0FKE68.1e-14753.82Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4... [more]
Q9ZSS61.2e-14551.60Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q9AXU42.2e-14453.43Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1[more]
P253066.4e-12846.62Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4... [more]
P536072.5e-11644.88L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain AT... [more]
Match NameE-valueIdentityDescription
A0A6J1ILR79.3e-23972.67Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477609 PE=3 SV=1[more]
A0A6J1IJC64.8e-23572.17Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477609 PE=3 SV=1[more]
A0A6J1ECR51.2e-23370.72Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433136 PE=3 SV=1[more]
A0A5D3C7Q33.0e-22971.12Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold20... [more]
A0A1S3CH383.0e-22971.12Threonine dehydratase OS=Cucumis melo OX=3656 GN=LOC103500853 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10050.18.3e-14751.60L-O-methylthreonine resistant 1 [more]
AT4G11640.19.8e-3933.22serine racemase [more]
AT3G61440.12.6e-0723.75cysteine synthase C1 [more]
AT3G03630.15.8e-0722.07cysteine synthase 26 [more]
AT3G61440.24.9e-0625.58cysteine synthase C1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005787Threonine dehydratase, biosyntheticTIGRFAMTIGR01124TIGR01124coord: 116..597
e-value: 2.2E-173
score: 575.4
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 130..417
e-value: 2.1E-73
score: 247.3
IPR001721Threonine dehydratase, ACT-like domainPFAMPF00585Thr_dehydrat_Ccoord: 433..519
e-value: 1.2E-5
score: 25.0
coord: 525..596
e-value: 2.1E-16
score: 59.5
IPR001721Threonine dehydratase, ACT-like domainPROSITEPS51672ACT_LIKEcoord: 533..609
score: 9.530398
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 126..420
e-value: 2.1E-100
score: 337.7
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 161..257
e-value: 2.1E-100
score: 337.7
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 120..460
IPR038110Threonine dehydratase, ACT-like domain superfamilyGENE3D3.40.1020.10Biosynthetic Threonine Deaminase; Domain 3coord: 437..609
e-value: 1.6E-40
score: 140.5
NoneNo IPR availablePIRSRPIRSR006278-2PIRSR006278-2coord: 145..319
e-value: 8.1E-4
score: 16.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..38
NoneNo IPR availablePANTHERPTHR48078:SF11THREONINE DEHYDRATASE, MITOCHONDRIALcoord: 83..598
NoneNo IPR availablePANTHERPTHR48078THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATEDcoord: 83..598
NoneNo IPR availableCDDcd01562Thr-dehydcoord: 127..421
e-value: 2.20133E-142
score: 415.732
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 438..522
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 520..597
IPR000634Serine/threonine dehydratase, pyridoxal-phosphate-binding sitePROSITEPS00165DEHYDRATASE_SER_THRcoord: 153..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012737.1Spg012737.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009097 isoleucine biosynthetic process
biological_process GO:0006567 threonine catabolic process
biological_process GO:0006520 cellular amino acid metabolic process
molecular_function GO:0004794 L-threonine ammonia-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding