Spg012588 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012588
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionOxidoreductase-like domain-containing protein
Locationscaffold1: 5624144 .. 5624548 (+)
RNA-Seq ExpressionSpg012588
SyntenySpg012588
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACTGATCGAGCACAATTTTACTCCGACTTCCATTTTTCCGTCATCTTTATCGAATCATAATTCAAATCGTCTCCGCCAATTAGGGCCAGAGAATGTCCCCTTGGCCATGAGACGAGTCCCTGCAAAAATAATTCCCAATCTCCAACCATCAGGCGTTTACCCTAAATCGATCATGGCTAGCGAGGGAACAAGAGAAATCCAGTCAGTTGATTCGAAGAAGAAAGGCGTGGAGGAGAAAGAAGAGAAAGAGAAGAGCGTGAAATTGCCGCCGCCGCCGGATAAGCCGTTGCCGGGAGATTGCTGTGGGAGTGGATGCGTTCGATGTGTGTGGGACGTGTATTACGAAGAGCTTGAAGCTTACAACAAGCTTTGCGAGAAAGGTTCGGCGCCCGATTCTTGA

mRNA sequence

ATGAAACTGATCGAGCACAATTTTACTCCGACTTCCATTTTTCCGTCATCTTTATCGAATCATAATTCAAATCGTCTCCGCCAATTAGGGCCAGAGAATGTCCCCTTGGCCATGAGACGAGTCCCTGCAAAAATAATTCCCAATCTCCAACCATCAGGCGTTTACCCTAAATCGATCATGGCTAGCGAGGGAACAAGAGAAATCCAGTCAGTTGATTCGAAGAAGAAAGGCGTGGAGGAGAAAGAAGAGAAAGAGAAGAGCGTGAAATTGCCGCCGCCGCCGGATAAGCCGTTGCCGGGAGATTGCTGTGGGAGTGGATGCGTTCGATGTGTGTGGGACGTGTATTACGAAGAGCTTGAAGCTTACAACAAGCTTTGCGAGAAAGGTTCGGCGCCCGATTCTTGA

Coding sequence (CDS)

ATGAAACTGATCGAGCACAATTTTACTCCGACTTCCATTTTTCCGTCATCTTTATCGAATCATAATTCAAATCGTCTCCGCCAATTAGGGCCAGAGAATGTCCCCTTGGCCATGAGACGAGTCCCTGCAAAAATAATTCCCAATCTCCAACCATCAGGCGTTTACCCTAAATCGATCATGGCTAGCGAGGGAACAAGAGAAATCCAGTCAGTTGATTCGAAGAAGAAAGGCGTGGAGGAGAAAGAAGAGAAAGAGAAGAGCGTGAAATTGCCGCCGCCGCCGGATAAGCCGTTGCCGGGAGATTGCTGTGGGAGTGGATGCGTTCGATGTGTGTGGGACGTGTATTACGAAGAGCTTGAAGCTTACAACAAGCTTTGCGAGAAAGGTTCGGCGCCCGATTCTTGA

Protein sequence

MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPENVPLAMRRVPAKIIPNLQPSGVYPKSIMASEGTREIQSVDSKKKGVEEKEEKEKSVKLPPPPDKPLPGDCCGSGCVRCVWDVYYEELEAYNKLCEKGSAPDS
Homology
BLAST of Spg012588 vs. NCBI nr
Match: XP_023518260.1 (uncharacterized protein LOC111781791 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 215.3 bits (547), Expect = 3.2e-52
Identity = 110/141 (78.01%), Postives = 119/141 (84.40%), Query Frame = 0

Query: 1   MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPEN----VPLAMRRVPAKIIPNLQPSGVYP 60
           MKLI HNF P+SIF SSLSN NSNRLRQ   EN    VPLAMRRVPAKIIPN Q S VYP
Sbjct: 1   MKLIHHNFIPSSIFSSSLSNRNSNRLRQFEAENVRPHVPLAMRRVPAKIIPNFQSSSVYP 60

Query: 61  KSIMASEGTREIQSVDSKKKGVEEKEEK---EKSVKLPPPPDKPLPGDCCGSGCVRCVWD 120
           KSIMAS+G REIQSVD ++ GVEEKE+K   +K+VKLPPPP+KPLPGDCCGSGCVRCVWD
Sbjct: 61  KSIMASDGAREIQSVDLQRTGVEEKEKKATEKKAVKLPPPPEKPLPGDCCGSGCVRCVWD 120

Query: 121 VYYEELEAYNKLCEKGSAPDS 135
           VYYEELE YNKLCEKGSA +S
Sbjct: 121 VYYEELEDYNKLCEKGSASNS 141

BLAST of Spg012588 vs. NCBI nr
Match: KAG6594792.1 (hypothetical protein SDJN03_11345, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026759.1 SPCP31B10.02, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 213.0 bits (541), Expect = 1.6e-51
Identity = 109/141 (77.30%), Postives = 118/141 (83.69%), Query Frame = 0

Query: 1   MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPEN----VPLAMRRVPAKIIPNLQPSGVYP 60
           MKLI HNF P+SI  SSLSN NSNRLRQ   EN    VPLAMRRVPAKIIPN Q S VYP
Sbjct: 1   MKLIHHNFIPSSILSSSLSNRNSNRLRQFEAENVRRYVPLAMRRVPAKIIPNFQSSSVYP 60

Query: 61  KSIMASEGTREIQSVDSKKKGVEEKEEK---EKSVKLPPPPDKPLPGDCCGSGCVRCVWD 120
           KSIMAS+G REIQSVD ++ GVEEKE+K   +K+VKLPPPP+KPLPGDCCGSGCVRCVWD
Sbjct: 61  KSIMASDGAREIQSVDLQRTGVEEKEKKATEKKAVKLPPPPEKPLPGDCCGSGCVRCVWD 120

Query: 121 VYYEELEAYNKLCEKGSAPDS 135
           VYYEELE YNKLCEKGSA +S
Sbjct: 121 VYYEELEDYNKLCEKGSASNS 141

BLAST of Spg012588 vs. NCBI nr
Match: XP_022963072.1 (uncharacterized protein LOC111463382 [Cucurbita moschata])

HSP 1 Score: 210.7 bits (535), Expect = 7.8e-51
Identity = 110/142 (77.46%), Postives = 119/142 (83.80%), Query Frame = 0

Query: 1   MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPEN----VPLAMRRVPAKIIPNLQPSGVYP 60
           MKLI HNF P+SIF SSLSN NSNRLRQ   EN    VPLAMRRVPAKIIPN Q S VYP
Sbjct: 1   MKLIHHNFIPSSIFSSSLSNRNSNRLRQFEAENVRRYVPLAMRRVPAKIIPNFQSSSVYP 60

Query: 61  KSIMASEGTREIQSVDSKKKGVEEKEEK---EKSVKL-PPPPDKPLPGDCCGSGCVRCVW 120
           KSIMAS+G REIQSVD ++ GVEEKE+K   +K+VKL PPPP+KPLPGDCCGSGCVRCVW
Sbjct: 61  KSIMASDGAREIQSVDLQRTGVEEKEKKATEKKAVKLPPPPPEKPLPGDCCGSGCVRCVW 120

Query: 121 DVYYEELEAYNKLCEKGSAPDS 135
           DVYYEELE YNKLCEKGSA +S
Sbjct: 121 DVYYEELEDYNKLCEKGSASNS 142

BLAST of Spg012588 vs. NCBI nr
Match: XP_023003942.1 (uncharacterized protein LOC111497385 [Cucurbita maxima])

HSP 1 Score: 208.4 bits (529), Expect = 3.9e-50
Identity = 107/140 (76.43%), Postives = 116/140 (82.86%), Query Frame = 0

Query: 1   MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPEN----VPLAMRRVPAKIIPNLQPSGVYP 60
           MKLI +NF P+SI  SSLSN NSNRLRQ   EN    VPLAMRR PAKIIPN Q S VYP
Sbjct: 1   MKLIHNNFIPSSILSSSLSNRNSNRLRQFEAENVWPHVPLAMRRFPAKIIPNFQSSSVYP 60

Query: 61  KSIMASEGTREIQSVDSKKKGVEEKEEK--EKSVKLPPPPDKPLPGDCCGSGCVRCVWDV 120
           KSIMAS+G RE QSVD ++ GVEEKE+K  EK+VKLPPPP+KPLPGDCCGSGCVRCVWDV
Sbjct: 61  KSIMASDGARETQSVDLQRTGVEEKEKKETEKAVKLPPPPEKPLPGDCCGSGCVRCVWDV 120

Query: 121 YYEELEAYNKLCEKGSAPDS 135
           YYEELE YNKLCEKGSA +S
Sbjct: 121 YYEELEDYNKLCEKGSASNS 140

BLAST of Spg012588 vs. NCBI nr
Match: XP_038881411.1 (uncharacterized protein LOC120072947 [Benincasa hispida])

HSP 1 Score: 205.7 bits (522), Expect = 2.5e-49
Identity = 104/133 (78.20%), Postives = 115/133 (86.47%), Query Frame = 0

Query: 1   MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPE----NVPLAMRRVPAKIIPNLQPSGVYP 60
           MKLIE NFTPTS+F SSLSN NSN LRQL PE    ++P AMRRVPAKIIPN Q   +YP
Sbjct: 1   MKLIEQNFTPTSLFLSSLSNLNSNCLRQLRPEYVRPHLPWAMRRVPAKIIPNFQSPRIYP 60

Query: 61  KSIMASEGTREIQ-SVDSKKKGVEEKEEKEKSVKLPPPPDKPLPGDCCGSGCVRCVWDVY 120
            SIMASEGTRE+Q SVDSK+KG E+KE+KEK+VKLPPPP+KPLPGDCCGSGCVRCVWD+Y
Sbjct: 61  NSIMASEGTREMQSSVDSKEKGTEKKEKKEKNVKLPPPPEKPLPGDCCGSGCVRCVWDIY 120

Query: 121 YEELEAYNKLCEK 129
           YEELE YNKLCEK
Sbjct: 121 YEELEDYNKLCEK 133

BLAST of Spg012588 vs. ExPASy Swiss-Prot
Match: Q9USH2 (UPF0651 protein P31B10.02, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCP31B10.02 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-06
Identity = 21/41 (51.22%), Postives = 30/41 (73.17%), Query Frame = 0

Query: 88  VKLPPPPDKPLPGDCCGSGCVRCVWDVYYEELEAYNKLCEK 129
           +++PP P++PL  +CC SGC  CVWDVY ++LE YN+   K
Sbjct: 52  IRVPPKPEEPL--NCCQSGCAICVWDVYADDLEEYNRARRK 90

BLAST of Spg012588 vs. ExPASy Swiss-Prot
Match: B1AS42 (NADH-cytochrome b5 reductase-like OS=Mus musculus OX=10090 GN=Cyb5rl PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 4.0e-05
Identity = 21/44 (47.73%), Postives = 29/44 (65.91%), Query Frame = 0

Query: 79  EEKEEKEKSVKLPPPPDKPLPGDCCGSGCVRCVWDVYYEELEAY 123
           E +EE++    L   P +PLP  CCGSGC  CV+D+YY +LE +
Sbjct: 3   ETEEEEDSEAWLRLKPVEPLPSQCCGSGCSPCVFDLYYRDLERW 46

BLAST of Spg012588 vs. ExPASy TrEMBL
Match: A0A6J1HE87 (uncharacterized protein LOC111463382 OS=Cucurbita moschata OX=3662 GN=LOC111463382 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.8e-51
Identity = 110/142 (77.46%), Postives = 119/142 (83.80%), Query Frame = 0

Query: 1   MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPEN----VPLAMRRVPAKIIPNLQPSGVYP 60
           MKLI HNF P+SIF SSLSN NSNRLRQ   EN    VPLAMRRVPAKIIPN Q S VYP
Sbjct: 1   MKLIHHNFIPSSIFSSSLSNRNSNRLRQFEAENVRRYVPLAMRRVPAKIIPNFQSSSVYP 60

Query: 61  KSIMASEGTREIQSVDSKKKGVEEKEEK---EKSVKL-PPPPDKPLPGDCCGSGCVRCVW 120
           KSIMAS+G REIQSVD ++ GVEEKE+K   +K+VKL PPPP+KPLPGDCCGSGCVRCVW
Sbjct: 61  KSIMASDGAREIQSVDLQRTGVEEKEKKATEKKAVKLPPPPPEKPLPGDCCGSGCVRCVW 120

Query: 121 DVYYEELEAYNKLCEKGSAPDS 135
           DVYYEELE YNKLCEKGSA +S
Sbjct: 121 DVYYEELEDYNKLCEKGSASNS 142

BLAST of Spg012588 vs. ExPASy TrEMBL
Match: A0A6J1KP06 (uncharacterized protein LOC111497385 OS=Cucurbita maxima OX=3661 GN=LOC111497385 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.9e-50
Identity = 107/140 (76.43%), Postives = 116/140 (82.86%), Query Frame = 0

Query: 1   MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPEN----VPLAMRRVPAKIIPNLQPSGVYP 60
           MKLI +NF P+SI  SSLSN NSNRLRQ   EN    VPLAMRR PAKIIPN Q S VYP
Sbjct: 1   MKLIHNNFIPSSILSSSLSNRNSNRLRQFEAENVWPHVPLAMRRFPAKIIPNFQSSSVYP 60

Query: 61  KSIMASEGTREIQSVDSKKKGVEEKEEK--EKSVKLPPPPDKPLPGDCCGSGCVRCVWDV 120
           KSIMAS+G RE QSVD ++ GVEEKE+K  EK+VKLPPPP+KPLPGDCCGSGCVRCVWDV
Sbjct: 61  KSIMASDGARETQSVDLQRTGVEEKEKKETEKAVKLPPPPEKPLPGDCCGSGCVRCVWDV 120

Query: 121 YYEELEAYNKLCEKGSAPDS 135
           YYEELE YNKLCEKGSA +S
Sbjct: 121 YYEELEDYNKLCEKGSASNS 140

BLAST of Spg012588 vs. ExPASy TrEMBL
Match: A0A6J1BUD5 (uncharacterized protein LOC111005446 OS=Momordica charantia OX=3673 GN=LOC111005446 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.2e-46
Identity = 106/144 (73.61%), Postives = 114/144 (79.17%), Query Frame = 0

Query: 1   MKLIEHNFTPTSIFPSSLSNHNSNRLRQLGPENV------PLAMRRVPAKIIPNLQPSGV 60
           MKLI   F PTS      SNH S+R  +L PENV      PLAMRRVPAKIIPNLQ S V
Sbjct: 1   MKLIGQKFNPTS------SNHYSDRRHRLRPENVHVRVHAPLAMRRVPAKIIPNLQSSSV 60

Query: 61  YPKSIMASEGTREIQSVDSKKKG--VEEKE----EKEKSVKLPPPPDKPLPGDCCGSGCV 120
           YPKSIMASEGTRE  SV+S+KKG  VEEKE    EKEK++KLPPPP+KPLPGDCCGSGCV
Sbjct: 61  YPKSIMASEGTRENLSVESQKKGVDVEEKEDKEKEKEKTLKLPPPPEKPLPGDCCGSGCV 120

Query: 121 RCVWDVYYEELEAYNKLCEKGSAP 133
           RCVWDVYYEELEAYNKLC+KGSAP
Sbjct: 121 RCVWDVYYEELEAYNKLCDKGSAP 138

BLAST of Spg012588 vs. ExPASy TrEMBL
Match: A0A0A0KKK8 (Glutaredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G504670 PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.5e-26
Identity = 64/77 (83.12%), Postives = 69/77 (89.61%), Query Frame = 0

Query: 60  MASEGTREIQ-SVDSKKKGVEE-----KEEKEKSVKLPPPPDKPLPGDCCGSGCVRCVWD 119
           MASEGTRE+Q SVDSKKKGVEE     ++EKE+ VKLPPPP+KPLPGDCCGSGCVRCVWD
Sbjct: 1   MASEGTREMQSSVDSKKKGVEEEKAEKEKEKEEMVKLPPPPEKPLPGDCCGSGCVRCVWD 60

Query: 120 VYYEELEAYNKLCEKGS 131
           VYYEELE YNKLCEKGS
Sbjct: 61  VYYEELEDYNKLCEKGS 77

BLAST of Spg012588 vs. ExPASy TrEMBL
Match: A0A5D3CL09 (UPF0651 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001550 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.5e-26
Identity = 64/83 (77.11%), Postives = 69/83 (83.13%), Query Frame = 0

Query: 60  MASEGTREIQ-SVDSKKKGVEE---------KEEKEKSVKLPPPPDKPLPGDCCGSGCVR 119
           MAS+ TRE+Q SVDSKKKG EE         ++EKEK VKLPPPP+KPLPGDCCGSGCVR
Sbjct: 1   MASDATREMQSSVDSKKKGAEEEKVEKEEEKEKEKEKIVKLPPPPEKPLPGDCCGSGCVR 60

Query: 120 CVWDVYYEELEAYNKLCEKGSAP 133
           CVWDVYYEELE YNKLCEKGSAP
Sbjct: 61  CVWDVYYEELEDYNKLCEKGSAP 83

BLAST of Spg012588 vs. TAIR 10
Match: AT5G17280.1 (CONTAINS InterPro DOMAIN/s: Oxidoreductase-like, N-terminal (InterPro:IPR019180); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 84.3 bits (207), Expect = 7.9e-17
Identity = 36/69 (52.17%), Postives = 49/69 (71.01%), Query Frame = 0

Query: 56  PKSIMASEGTREIQSVDSKKKGVEEKEEKEKSVKLPPPPDKPLPGDCCGSGCVRCVWDVY 115
           P ++  +E    +++   +    E +++KE  V +PPPP+KP PGDCCGSGCVRCVWDVY
Sbjct: 58  PVTMATTESKNLVEASKEETNKKETEDKKEVGVSVPPPPEKPEPGDCCGSGCVRCVWDVY 117

Query: 116 YEELEAYNK 125
           Y+ELE YNK
Sbjct: 118 YDELEDYNK 126

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023518260.13.2e-5278.01uncharacterized protein LOC111781791 [Cucurbita pepo subsp. pepo][more]
KAG6594792.11.6e-5177.30hypothetical protein SDJN03_11345, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022963072.17.8e-5177.46uncharacterized protein LOC111463382 [Cucurbita moschata][more]
XP_023003942.13.9e-5076.43uncharacterized protein LOC111497385 [Cucurbita maxima][more]
XP_038881411.12.5e-4978.20uncharacterized protein LOC120072947 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9USH21.6e-0651.22UPF0651 protein P31B10.02, mitochondrial OS=Schizosaccharomyces pombe (strain 97... [more]
B1AS424.0e-0547.73NADH-cytochrome b5 reductase-like OS=Mus musculus OX=10090 GN=Cyb5rl PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HE873.8e-5177.46uncharacterized protein LOC111463382 OS=Cucurbita moschata OX=3662 GN=LOC1114633... [more]
A0A6J1KP061.9e-5076.43uncharacterized protein LOC111497385 OS=Cucurbita maxima OX=3661 GN=LOC111497385... [more]
A0A6J1BUD52.2e-4673.61uncharacterized protein LOC111005446 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A0A0KKK81.5e-2683.12Glutaredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G50467... [more]
A0A5D3CL092.5e-2677.11UPF0651 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G0015... [more]
Match NameE-valueIdentityDescription
AT5G17280.17.9e-1752.17CONTAINS InterPro DOMAIN/s: Oxidoreductase-like, N-terminal (InterPro:IPR019180)... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019180Oxidoreductase-like, N-terminalPFAMPF09791Oxidored-likecoord: 88..127
e-value: 1.8E-14
score: 53.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 60..100
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..91
IPR039251Oxidoreductase-like domain-containing protein 1PANTHERPTHR21193OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1coord: 52..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012588.1Spg012588.1mRNA