Spg012569 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg012569 vs. NCBI nr
Match: XP_023518138.1 (cytochrome P450 84A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 911.8 bits (2355), Expect = 2.7e-261
Identity = 440/507 (86.79%), Postives = 472/507 (93.10%), Query Frame = 0

Query: 9   HMPLFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGI 68
           HM L FT+PLLFLLA +FR RRKLPYPPGP GLPF+GNMLM D LTHRGLA LAKRYGGI
Sbjct: 16  HMALIFTIPLLFLLALIFRLRRKLPYPPGPTGLPFLGNMLMTDQLTHRGLAKLAKRYGGI 75

Query: 69  FHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQ 128
           FHMRMGFLHMV +SNPD+AR+VLQVHDNICSNRP TIAISYLTYD+ADMAFAHYGPFWRQ
Sbjct: 76  FHMRMGFLHMVTISNPDMAREVLQVHDNICSNRPVTIAISYLTYDQADMAFAHYGPFWRQ 135

Query: 129 MRKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAAFG 188
           MRK+CVMKLFSRKRAESWASVRDEVD+ +R V  + GT VN+GE VFELT +IIYRAAFG
Sbjct: 136 MRKLCVMKLFSRKRAESWASVRDEVDSTVRHVATQLGTVVNVGERVFELTMNIIYRAAFG 195

Query: 189 TSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLDKFIDTII 248
           TS VQGQDEFI+ILQEFS+LFGAFNIADFIP+L WIDPQGLNNRLVKARQSLDKFIDTII
Sbjct: 196 TSTVQGQDEFIKILQEFSKLFGAFNIADFIPNLSWIDPQGLNNRLVKARQSLDKFIDTII 255

Query: 249 DEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRDNIKAIIM 308
           DEHME KR R LSNVKDDEI+TDMVDELLAFYDE  +VNE +D QN IKLNRDNIKAIIM
Sbjct: 256 DEHMEKKRIRKLSNVKDDEIETDMVDELLAFYDEQVSVNELDDSQNGIKLNRDNIKAIIM 315

Query: 309 DVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTFLKCCL 368
           DVMFGGTETVASAIEW +SELMR+P+DLKKAQQELA+VVG  RRVEE+D ENL FLKCC+
Sbjct: 316 DVMFGGTETVASAIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDMENLAFLKCCI 375

Query: 369 KETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFL 428
           KETLRLHPPIPLLLHETAED+VV+GYFIPAKSRV+VNAWAIGRDPASWD+AETFRPARFL
Sbjct: 376 KETLRLHPPIPLLLHETAEDVVVAGYFIPAKSRVIVNAWAIGRDPASWDEAETFRPARFL 435

Query: 429 EQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPSEMDM 488
           +QGV DFKG NFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKP+EMDM
Sbjct: 436 KQGVADFKGGNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPNEMDM 495

Query: 489 GDVFGLTAPRATRLVAVPTKRVLCPLV 516
           GD+FGLTAPRATRLVAVPTKRVLCPLV
Sbjct: 496 GDIFGLTAPRATRLVAVPTKRVLCPLV 522

BLAST of Spg012569 vs. NCBI nr
Match: XP_023003875.1 (cytochrome P450 84A1 [Cucurbita maxima])

HSP 1 Score: 908.7 bits (2347), Expect = 2.3e-260
Identity = 437/506 (86.36%), Postives = 474/506 (93.68%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGIF 69
           M L FTLPLLFLLA +FR RRKLPYPPGPKGLPF+GNMLMMD LTHRGLA LAKRYGGIF
Sbjct: 1   MALIFTLPLLFLLALIFRLRRKLPYPPGPKGLPFLGNMLMMDQLTHRGLAKLAKRYGGIF 60

Query: 70  HMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQM 129
           HMRMGFLHMV +SNPD+ARQVLQV+DN+CSNRP TIAISYLTYD+ADMAFAHYGPFWRQM
Sbjct: 61  HMRMGFLHMVTISNPDMARQVLQVNDNVCSNRPVTIAISYLTYDQADMAFAHYGPFWRQM 120

Query: 130 RKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAAFGT 189
           RK+CVMKLFSRKRAESWASVRDE+D+ +R V  + GT VN+GE VFELT +IIYRAAFGT
Sbjct: 121 RKLCVMKLFSRKRAESWASVRDEIDSTVRHVAAQLGTVVNVGERVFELTMNIIYRAAFGT 180

Query: 190 SAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLDKFIDTIID 249
           S VQGQDEFI+ILQEFS+LFGAFNIADF+P+L WIDPQGLNNRLVKARQSLDKFIDTIID
Sbjct: 181 STVQGQDEFIKILQEFSKLFGAFNIADFMPNLSWIDPQGLNNRLVKARQSLDKFIDTIID 240

Query: 250 EHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRDNIKAIIMD 309
           EHME KR R LSNVKDD+I+TDMVDELLAFYDE  +VNE +D QN IKLNRDNIKAIIMD
Sbjct: 241 EHMEKKRIRKLSNVKDDKIETDMVDELLAFYDEQVSVNELDDSQNGIKLNRDNIKAIIMD 300

Query: 310 VMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTFLKCCLK 369
           VMFGGTETVASAIEW +SELMR+P+DLKKAQQELA+VVG  RRVEE+D ENL FLKCC+K
Sbjct: 301 VMFGGTETVASAIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDLENLAFLKCCIK 360

Query: 370 ETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFLE 429
           ETLRLHPPIPLLLHETAED+VV+GYFIPAKSRV+VNAWAIGRDPASWDDAETFRP+RFL+
Sbjct: 361 ETLRLHPPIPLLLHETAEDVVVAGYFIPAKSRVIVNAWAIGRDPASWDDAETFRPSRFLK 420

Query: 430 QGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPSEMDMG 489
           QGV DFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKP+EMDMG
Sbjct: 421 QGVADFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPNEMDMG 480

Query: 490 DVFGLTAPRATRLVAVPTKRVLCPLV 516
           D+FGLT+PRATRLVAVPT+RVLCPLV
Sbjct: 481 DIFGLTSPRATRLVAVPTQRVLCPLV 506

BLAST of Spg012569 vs. NCBI nr
Match: XP_022962687.1 (cytochrome P450 84A1 [Cucurbita moschata])

HSP 1 Score: 904.4 bits (2336), Expect = 4.4e-259
Identity = 437/506 (86.36%), Postives = 471/506 (93.08%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGIF 69
           M L FT+PLLFLLA +F  RRKLPYPPGP+GLPF+GNMLMMD LTHRGLA LAKRYGGIF
Sbjct: 1   MALIFTIPLLFLLALIFWLRRKLPYPPGPRGLPFLGNMLMMDQLTHRGLAKLAKRYGGIF 60

Query: 70  HMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQM 129
           HMRMGFLHMV +S+PD+ARQVLQVHDNICSNRP TIAISYLTY++ADMAFAHYGPFWRQM
Sbjct: 61  HMRMGFLHMVTISDPDMARQVLQVHDNICSNRPVTIAISYLTYNQADMAFAHYGPFWRQM 120

Query: 130 RKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAAFGT 189
           RK+CVMKLFSRKRAESWASVRDEVD+ +R V  + GT VN+GE VFELT +IIYRAAFGT
Sbjct: 121 RKLCVMKLFSRKRAESWASVRDEVDSTVRHVATQLGTVVNVGERVFELTMNIIYRAAFGT 180

Query: 190 SAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLDKFIDTIID 249
           S VQGQDEFI+ILQEFS+LFGAFNIADFIP+L WIDPQGLNNRLVKARQSLDKFIDTIID
Sbjct: 181 STVQGQDEFIKILQEFSKLFGAFNIADFIPNLSWIDPQGLNNRLVKARQSLDKFIDTIID 240

Query: 250 EHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRDNIKAIIMD 309
           EHME KR R LSNVKDDEI+TDMVDEL+AFYDE  +VNE +D QN IKLNRDNIKAIIMD
Sbjct: 241 EHMEKKRIRKLSNVKDDEIETDMVDELVAFYDEQVSVNELDDSQNGIKLNRDNIKAIIMD 300

Query: 310 VMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTFLKCCLK 369
           VMFGGTETVAS IEW +SELMR+P+DLKKAQQELA+VVG  RRVEE+D ENL FLKCC+K
Sbjct: 301 VMFGGTETVASVIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDMENLAFLKCCIK 360

Query: 370 ETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFLE 429
           ETLRLHPPIPLLLHETAED VV+GYFIPAKSRV+VNAWAIGRDPASWDDAETFRPARFL+
Sbjct: 361 ETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVIVNAWAIGRDPASWDDAETFRPARFLK 420

Query: 430 QGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPSEMDMG 489
           QGV DFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKP+EMDMG
Sbjct: 421 QGVADFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPNEMDMG 480

Query: 490 DVFGLTAPRATRLVAVPTKRVLCPLV 516
           D+FGLTAPRATRLVA+PTKRVLCPLV
Sbjct: 481 DIFGLTAPRATRLVALPTKRVLCPLV 506

BLAST of Spg012569 vs. NCBI nr
Match: XP_038883545.1 (cytochrome P450 84A1-like [Benincasa hispida])

HSP 1 Score: 900.6 bits (2326), Expect = 6.3e-258
Identity = 454/518 (87.64%), Postives = 471/518 (90.93%), Query Frame = 0

Query: 3   SLLQPLHMPLFFTLPLLFLLAFLF-RFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANL 62
           SLLQPL+M  FFTLPLLFLL  LF  FRRKLP PPGPKGLPFIGNMLMMD LTHRGLA L
Sbjct: 6   SLLQPLNMLFFFTLPLLFLLTLLFPPFRRKLPLPPGPKGLPFIGNMLMMDQLTHRGLARL 65

Query: 63  AKRYGGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAH 122
           A RYGGIFHMRMGFLHMV VSNPD+ARQVLQVHD ICSNRPATIAI+YLTY+RADMAFAH
Sbjct: 66  AARYGGIFHMRMGFLHMVTVSNPDIARQVLQVHDGICSNRPATIAIAYLTYNRADMAFAH 125

Query: 123 YGPFWRQMRKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDI 182
           YGPFWRQMRK+CVMKLFSRKRAESW SVRDEVD  I KV  RTGTAVNIGELVFELTRDI
Sbjct: 126 YGPFWRQMRKLCVMKLFSRKRAESWESVRDEVDNTIGKVAVRTGTAVNIGELVFELTRDI 185

Query: 183 IYRAAFGTSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLD 242
           IYRAAFGTSA QGQDEFIRILQEFS+LFGAFNI DFIP LGWIDPQGLNNRLVKAR+SLD
Sbjct: 186 IYRAAFGTSAEQGQDEFIRILQEFSQLFGAFNITDFIPMLGWIDPQGLNNRLVKARRSLD 245

Query: 243 KFIDTIIDEHMENKRKRTLSN----VKDDEIDTDMVDELLAFYDEHATV-NESNDQQNTI 302
           KFID+IID+H    RK+T S     VKD EID+DMVDELLAFY E +T  N S D QN  
Sbjct: 246 KFIDSIIDDH--TNRKKTTSKLPNYVKDHEIDSDMVDELLAFYSEDSTASNTSEDLQNNF 305

Query: 303 KLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEET 362
           KLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMR+PEDLKKAQQELA+VVG ERRVEE+
Sbjct: 306 KLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELAQVVGLERRVEES 365

Query: 363 DFENLTFLKCCLKETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASW 422
           DFENLTF KCCLKETLRLHPPIPLLLHETAED VV+GYFIPAKSRVMVNAWAIGRDPASW
Sbjct: 366 DFENLTFFKCCLKETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVMVNAWAIGRDPASW 425

Query: 423 DDAETFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWE 482
           DDAETFRPARFLEQGVPDFKG+NFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCF WE
Sbjct: 426 DDAETFRPARFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFNWE 485

Query: 483 LPDGMKPSEMDMGDVFGLTAPRATRLVAVPTKRVLCPL 515
           LPDGMKPSEMDM D FGLTAPRATRLVAVPTKRVLCPL
Sbjct: 486 LPDGMKPSEMDMTDSFGLTAPRATRLVAVPTKRVLCPL 521

BLAST of Spg012569 vs. NCBI nr
Match: KAG7026756.1 (Cytochrome P450 84A1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 879.4 bits (2271), Expect = 1.5e-251
Identity = 430/506 (84.98%), Postives = 459/506 (90.71%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGIF 69
           M L FT+PLLFLLA +FR RRKLPYPPGP+GLPF+GNMLMMD LTHRGLA LAKRYGGIF
Sbjct: 1   MALIFTIPLLFLLALIFRLRRKLPYPPGPRGLPFLGNMLMMDQLTHRGLAKLAKRYGGIF 60

Query: 70  HMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQM 129
           HMRMGFLHM              VHDNICSNRP TIAISYLTYD+ADMAFAHYGPFWRQM
Sbjct: 61  HMRMGFLHM--------------VHDNICSNRPVTIAISYLTYDQADMAFAHYGPFWRQM 120

Query: 130 RKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAAFGT 189
           RK+CVMKLFSRKRAESWASVRDEVD+ +R V  + GT VN+GE VFELT +IIYRAAFGT
Sbjct: 121 RKLCVMKLFSRKRAESWASVRDEVDSTVRHVATQLGTVVNVGERVFELTMNIIYRAAFGT 180

Query: 190 SAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLDKFIDTIID 249
           S VQGQDEFI+ILQEFS+LFGAFNIADFIP+L WIDPQGLNNRLVKARQSLDKFIDTIID
Sbjct: 181 STVQGQDEFIKILQEFSKLFGAFNIADFIPNLSWIDPQGLNNRLVKARQSLDKFIDTIID 240

Query: 250 EHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRDNIKAIIMD 309
           EHME KR R LSNVKDDEI+TDMVDEL+AFYDE  +VNE +D QN IKLNRDNIKAIIMD
Sbjct: 241 EHMEKKRIRKLSNVKDDEIETDMVDELVAFYDEQVSVNELDDSQNGIKLNRDNIKAIIMD 300

Query: 310 VMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTFLKCCLK 369
           VMFGGTETVASAIEW +SELMR+P+DLKKAQQELA+VVG  RRVEE+D ENL FLKCC+K
Sbjct: 301 VMFGGTETVASAIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDMENLAFLKCCIK 360

Query: 370 ETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFLE 429
           ETLRLHPPIPLLLHETAED VV+GYFIPAKSRV+VNAWAIGRDPASWDDAE FRPARFL+
Sbjct: 361 ETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVIVNAWAIGRDPASWDDAEMFRPARFLK 420

Query: 430 QGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPSEMDMG 489
           QGV DFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKP+EMDMG
Sbjct: 421 QGVADFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPNEMDMG 480

Query: 490 DVFGLTAPRATRLVAVPTKRVLCPLV 516
           D+FGLTAPRATRLVAVPTKRVLCPLV
Sbjct: 481 DIFGLTAPRATRLVAVPTKRVLCPLV 492

BLAST of Spg012569 vs. ExPASy Swiss-Prot
Match: Q42600 (Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 5.3e-223
Identity = 378/505 (74.85%), Postives = 434/505 (85.94%), Query Frame = 0

Query: 12  LFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGIFHM 71
           L   + L   ++F+ R RR+ PYPPGP+G P IGNMLMMD LTHRGLANLAK+YGG+ H+
Sbjct: 19  LVIVVSLFIFISFITR-RRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLCHL 78

Query: 72  RMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQMRK 131
           RMGFLHM AVS+P+VARQVLQV D++ SNRPATIAISYLTYDRADMAFAHYGPFWRQMRK
Sbjct: 79  RMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMRK 138

Query: 132 VCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAAFGTSA 191
           VCVMK+FSRKRAESWASVRDEVD  +R V+   G  +N+GE +F LTR+I YRAAFG++ 
Sbjct: 139 VCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGKPINVGEQIFALTRNITYRAAFGSAC 198

Query: 192 VQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLDKFIDTIIDEH 251
            +GQDEFIRILQEFS+LFGAFN+ADFIP  GWIDPQG+N RLVKAR  LD FID IIDEH
Sbjct: 199 EKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDGFIDDIIDEH 258

Query: 252 MENKRKRTLSNVKD-DEIDTDMVDELLAFYDEHA-TVNESNDQQNTIKLNRDNIKAIIMD 311
           M  K+K   + V D D +DTDMVD+LLAFY E A  V+E+ D QN+IKL RDNIKAIIMD
Sbjct: 259 M--KKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRDNIKAIIMD 318

Query: 312 VMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTFLKCCLK 371
           VMFGGTETVASAIEWA++EL+R+PEDLK+ QQELAEVVG +RRVEE+D E LT+LKC LK
Sbjct: 319 VMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLK 378

Query: 372 ETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFLE 431
           ETLR+HPPIPLLLHETAED  + G+FIP KSRVM+NA+AIGRDP SW D +TFRP+RFLE
Sbjct: 379 ETLRMHPPIPLLLHETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLE 438

Query: 432 QGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPSEMDMG 491
            GVPDFKGSNFEFIPFGSGRRSCPGMQLGLY L++AVAH+LHCFTW+LPDGMKPSE+DM 
Sbjct: 439 PGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMN 498

Query: 492 DVFGLTAPRATRLVAVPTKRVLCPL 515
           DVFGLTAP+ATRL AVPT R++C L
Sbjct: 499 DVFGLTAPKATRLFAVPTTRLICAL 520

BLAST of Spg012569 vs. ExPASy Swiss-Prot
Match: F4JW83 (Cytochrome P450 84A4 OS=Arabidopsis thaliana OX=3702 GN=CYP84A4 PE=1 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 1.2e-179
Identity = 314/515 (60.97%), Postives = 388/515 (75.34%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLFRFRRKL---PYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYG 69
           M L   +PLL  L      RR++   PYPPGPKGLP IGN+LMM+   HRGLA L++ YG
Sbjct: 5   MTLIVLVPLLLFLFPHLLLRRQMLLKPYPPGPKGLPVIGNILMMNQFNHRGLAKLSRIYG 64

Query: 70  GIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFW 129
           G+ H+R+GF H+  VS+PD+ARQVLQV D++ SNRP TIAI YLTY  +D+AF +YGPFW
Sbjct: 65  GLLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIAIRYLTYGGSDLAFCNYGPFW 124

Query: 130 RQMRKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAA 189
           R+MRK+ VM LFSRKRAESW SV +EV  ++R V    G  +NI +L F L+RDI +RAA
Sbjct: 125 RRMRKLYVMMLFSRKRAESWVSVDEEVHKSVRLVASNVGKPLNICKLAFSLSRDITFRAA 184

Query: 190 FGTSAVQGQ----DEFIRILQEFSRLFGAFNIADFIPS-LGWIDPQGLNNRLVKARQSLD 249
           FG+S+        DEF+ I+QEFS+LFG FN+AD++PS L WIDPQG+N R+ KAR+SLD
Sbjct: 185 FGSSSSTSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWLSWIDPQGINGRVEKARKSLD 244

Query: 250 KFIDTIIDEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRD 309
            FI+++ID+H+ +K+KR   NV +   +TDMVD+LLAFY+E   VN S       K+N D
Sbjct: 245 GFIESVIDDHL-HKKKREHDNVDE---ETDMVDQLLAFYEEEVKVNNS-----VTKINLD 304

Query: 310 NIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFER-RVEETDFEN 369
           NIK IIMDVMFGGTETVA AIEW ++E++R+PE++K+ Q EL  VVG +R RVE+T  E 
Sbjct: 305 NIKGIIMDVMFGGTETVALAIEWVLTEILRSPENMKRVQDELTSVVGLDRWRVEDTHLEK 364

Query: 370 LTFLKCCLKETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAE 429
           LTFLKC LKETLRLHPP PLLLHET +D  +SGYFIP  SRVMVN +A+GRDP SW D E
Sbjct: 365 LTFLKCILKETLRLHPPFPLLLHETVKDTEISGYFIPKGSRVMVNTYALGRDPNSWSDPE 424

Query: 430 TFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDG 489
           +F P RFL    PD KG+NFEF+PFGSGRRSCPGMQLGLY  E+AVAHLLHCFTW LPDG
Sbjct: 425 SFNPGRFLNPIAPDLKGNNFEFVPFGSGRRSCPGMQLGLYAFELAVAHLLHCFTWSLPDG 484

Query: 490 MKPSEMDMGDVFGLTAPRATRLVAVPTKRVLCPLV 516
           M P ++D  +  GLT P+A  LVAVPT R+LCP+V
Sbjct: 485 MNPGDVDTVEGPGLTVPKAIPLVAVPTTRLLCPIV 510

BLAST of Spg012569 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 390.6 bits (1002), Expect = 2.8e-107
Identity = 207/494 (41.90%), Postives = 309/494 (62.55%), Query Frame = 0

Query: 12  LFFTLPLLFLLAFLFRFRRKLP-YPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGIFH 71
           LF  + L F L  L   R K P  PP P  LP IGN+  + +L HR L +LA   G +  
Sbjct: 8   LFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLIL 67

Query: 72  MRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQMR 131
           + +G +  + VS  ++A ++L+ HD I ++RP+T A   + YD  D+AF+ YG +WRQ+R
Sbjct: 68  LHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVR 127

Query: 132 KVCVMKLFSRKRAESWASVR-DEVDTAIRKVTG--RTGTAVNIGELVFELTRDIIYRAAF 191
           K+CV++L S KR  S+ S+R +EV   + +++    TG AVN+ EL+  L+   I R AF
Sbjct: 128 KICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAF 187

Query: 192 GTSAVQGQDE----FIRILQEFSRLFGAFNIADFIPSLGWIDP-QGLNNRLVKARQSLDK 251
           G    +G++E    F  +  E + L GAF + D+ PS  W+D   G++ RL +    LD 
Sbjct: 188 G-KKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDA 247

Query: 252 FIDTIIDEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRDN 311
           F+D +ID+H+ +++    +N  D     D+VD LL         +   D    + LNR+N
Sbjct: 248 FVDHVIDDHLLSRK----ANGSDGVEQKDLVDVLL---------HLQKDSSLGVHLNRNN 307

Query: 312 IKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLT 371
           +KA+I+D+  GGT+T A  +EWAM+EL+++P+ ++KAQQE+  VVG + +VEE D   L 
Sbjct: 308 LKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLH 367

Query: 372 FLKCCLKETLRLHPPIPLLL-HETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAET 431
           +LK  +KETLRLHP  PLL+  E+  D+V+ GY IPAK+RV +NAWAIGRDP SW++AE 
Sbjct: 368 YLKLIIKETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEE 427

Query: 432 FRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGM 491
           F P RF+   V DFKG +F+ IPFG+GRR CPG+  G+  +E+++A+LL+ F WELP  +
Sbjct: 428 FLPERFVNNSV-DFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDL 486

Query: 492 KPSEMDMGDVFGLT 496
              ++DM +  G+T
Sbjct: 488 TKEDLDMSEAVGIT 486

BLAST of Spg012569 vs. ExPASy Swiss-Prot
Match: A6YIH8 (Premnaspirodiene oxygenase OS=Hyoscyamus muticus OX=35626 GN=CYP71D55 PE=1 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 7.6e-105
Identity = 209/507 (41.22%), Postives = 310/507 (61.14%), Query Frame = 0

Query: 13  FFTLPLLFL-LAFLFRFRR-------KLPYPPGPKGLPFIGNML-MMDHLTHRGLANLAK 72
           FF+L  +FL L+FLF  R+           PPGP  LP +G+ML M+  L H  L +LAK
Sbjct: 3   FFSLVSIFLFLSFLFLLRKWKNSNSQSKKLPPGPWKLPLLGSMLHMVGGLPHHVLRDLAK 62

Query: 73  RYGGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYG 132
           +YG + H+++G +  V V++PD+A++VL+ HD   ++RP  +A   + Y+R+D+AF  YG
Sbjct: 63  KYGPLMHLQLGEVSAVVVTSPDMAKEVLKTHDIAFASRPKLLAPEIVCYNRSDIAFCPYG 122

Query: 133 PFWRQMRKVCVMKLFSRKRAESWASV-RDEVDTAIRKVTGRTGTAVNIGELVFELTRDII 192
            +WRQMRK+CV+++ S K   S++S+ RDEV   +  V   T   VN  E +F  T  + 
Sbjct: 123 DYWRQMRKICVLEVLSAKNVRSFSSIRRDEVLRLVNFVRSSTSEPVNFTERLFLFTSSMT 182

Query: 193 YRAAFGTSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDP-QGLNNRLVKARQSLD 252
            R+AFG    + Q+ FI++++E   L G F++AD  PSL ++    G+  +++KA   +D
Sbjct: 183 CRSAFG-KVFKEQETFIQLIKEVIGLAGGFDVADIFPSLKFLHVLTGMEGKIMKAHHKVD 242

Query: 253 KFIDTIIDEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRD 312
             ++ +I+EH +N      +     E   D++D LL            ND      +  D
Sbjct: 243 AIVEDVINEHKKNLAMGKTNGALGGE---DLIDVLLRL---------MNDGGLQFPITND 302

Query: 313 NIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENL 372
           NIKAII D+   GTET +S + WAM ++MRNP  L KAQ E+ E    +   +E D E L
Sbjct: 303 NIKAIIFDMFAAGTETSSSTLVWAMVQMMRNPTILAKAQAEVREAFKGKETFDENDVEEL 362

Query: 373 TFLKCCLKETLRLHPPIPLLL-HETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAE 432
            +LK  +KETLRLHPP+PLL+  E  E+  ++GY IP K++VMVN WA+GRDP  WDDA+
Sbjct: 363 KYLKLVIKETLRLHPPVPLLVPRECREETEINGYTIPVKTKVMVNVWALGRDPKYWDDAD 422

Query: 433 TFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDG 492
            F+P RF EQ   DF G+NFE++PFG GRR CPG+  GL  + + +A LL+ F W+LP G
Sbjct: 423 NFKPERF-EQCSVDFIGNNFEYLPFGGGRRICPGISFGLANVYLPLAQLLYHFDWKLPTG 482

Query: 493 MKPSEMDMGDVFGLTAPRATRLVAVPT 508
           M+P ++D+ ++ G+TA R + L+ V T
Sbjct: 483 MEPKDLDLTELVGVTAARKSDLMLVAT 495

BLAST of Spg012569 vs. ExPASy Swiss-Prot
Match: A0A0N9HT29 (Desmethyl-deoxy-podophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP71BE54 PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.2e-102
Identity = 208/512 (40.62%), Postives = 307/512 (59.96%), Query Frame = 0

Query: 1   MDSLLQPLHMPLFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNM--LMMDHLTHRGL 60
           M+ L  PL   L   + LLF+L       +    PPGP+ LP IG++  L+ D L H  L
Sbjct: 1   MEFLSFPLSSALL--IILLFMLVKKAIQNKNSKLPPGPRKLPVIGSLHHLIGDGLPHHAL 60

Query: 61  ANLAKRYGGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMA 120
            NLAK++G + H+++G    + VS+  +AR ++  HD + +NR    A   L Y    + 
Sbjct: 61  RNLAKKHGPLMHLQLGENSTLVVSSSKMARAIMSTHDLMFANRTVQFATDILLYGGKGIG 120

Query: 121 FAHYGPFWRQMRKVCVMKLFSRKRAESWASVR-DEVDTAIRKVTGRTGTAVNIGELVFEL 180
            A YG +WRQ+RK+CV++L S KR +S+ +VR +E+   IR ++  + + VN  E++  L
Sbjct: 121 LAPYGDYWRQIRKICVLELLSAKRVQSFQTVREEEISNLIRSISAES-SKVNFSEMITSL 180

Query: 181 TRDIIYRAAFGTSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDP-QGLNNRLVKA 240
           T DII RAAFG    + +  F+ +++E  +L G F+IA   PSL  +    G   +L + 
Sbjct: 181 TNDIIARAAFGEKC-KDKHAFLSLIEEGIQLAGGFDIAGLFPSLKLLHVITGKKRQLERV 240

Query: 241 RQSLDKFIDTIIDEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTI 300
              LD+ +  II    ENK KR  S + +D+ + ++VD L         V    + Q  +
Sbjct: 241 HNELDRILVDII---KENKEKRRASKLNEDKYEENLVDVL---------VRVQENTQLEV 300

Query: 301 KLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEET 360
            L  DN+KA+I+D+   G+ET ++ IEWAMSE+++NP+ +KKAQ E+  V    +++ ET
Sbjct: 301 PLANDNLKAVILDIFVAGSETSSTTIEWAMSEMLKNPKVMKKAQAEVRRVFSGNKKINET 360

Query: 361 DFENLTFLKCCLKETLRLHPPIPLLL-HETAEDLVVSGYFIPAKSRVMVNAWAIGRDPAS 420
           + + L++LK  LKETLRLH P PLL+  E  E   + GY IP K+ VMVNAWAIGRDP +
Sbjct: 361 EIQELSYLKLVLKETLRLHAPAPLLIPRECRESCEIDGYEIPKKTMVMVNAWAIGRDPEN 420

Query: 421 WDDAETFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTW 480
           W + + F P RF    V D+KG+NFE+IPFG+GRR CPGM  G+  +E+ +A LL+ F W
Sbjct: 421 WSNGDRFEPERFDGISV-DYKGTNFEYIPFGAGRRICPGMLFGMANVEVPLARLLYHFDW 480

Query: 481 ELPDGMKPSEMDMGDVFGLTAPRATRLVAVPT 508
           ELP+G+KP E+DM + FG T  R   L  +PT
Sbjct: 481 ELPNGIKPEELDMDETFGTTVRRKNHLYLIPT 495

BLAST of Spg012569 vs. ExPASy TrEMBL
Match: A0A6J1KT07 (cytochrome P450 84A1 OS=Cucurbita maxima OX=3661 GN=LOC111497337 PE=3 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 1.1e-260
Identity = 437/506 (86.36%), Postives = 474/506 (93.68%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGIF 69
           M L FTLPLLFLLA +FR RRKLPYPPGPKGLPF+GNMLMMD LTHRGLA LAKRYGGIF
Sbjct: 1   MALIFTLPLLFLLALIFRLRRKLPYPPGPKGLPFLGNMLMMDQLTHRGLAKLAKRYGGIF 60

Query: 70  HMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQM 129
           HMRMGFLHMV +SNPD+ARQVLQV+DN+CSNRP TIAISYLTYD+ADMAFAHYGPFWRQM
Sbjct: 61  HMRMGFLHMVTISNPDMARQVLQVNDNVCSNRPVTIAISYLTYDQADMAFAHYGPFWRQM 120

Query: 130 RKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAAFGT 189
           RK+CVMKLFSRKRAESWASVRDE+D+ +R V  + GT VN+GE VFELT +IIYRAAFGT
Sbjct: 121 RKLCVMKLFSRKRAESWASVRDEIDSTVRHVAAQLGTVVNVGERVFELTMNIIYRAAFGT 180

Query: 190 SAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLDKFIDTIID 249
           S VQGQDEFI+ILQEFS+LFGAFNIADF+P+L WIDPQGLNNRLVKARQSLDKFIDTIID
Sbjct: 181 STVQGQDEFIKILQEFSKLFGAFNIADFMPNLSWIDPQGLNNRLVKARQSLDKFIDTIID 240

Query: 250 EHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRDNIKAIIMD 309
           EHME KR R LSNVKDD+I+TDMVDELLAFYDE  +VNE +D QN IKLNRDNIKAIIMD
Sbjct: 241 EHMEKKRIRKLSNVKDDKIETDMVDELLAFYDEQVSVNELDDSQNGIKLNRDNIKAIIMD 300

Query: 310 VMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTFLKCCLK 369
           VMFGGTETVASAIEW +SELMR+P+DLKKAQQELA+VVG  RRVEE+D ENL FLKCC+K
Sbjct: 301 VMFGGTETVASAIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDLENLAFLKCCIK 360

Query: 370 ETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFLE 429
           ETLRLHPPIPLLLHETAED+VV+GYFIPAKSRV+VNAWAIGRDPASWDDAETFRP+RFL+
Sbjct: 361 ETLRLHPPIPLLLHETAEDVVVAGYFIPAKSRVIVNAWAIGRDPASWDDAETFRPSRFLK 420

Query: 430 QGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPSEMDMG 489
           QGV DFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKP+EMDMG
Sbjct: 421 QGVADFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPNEMDMG 480

Query: 490 DVFGLTAPRATRLVAVPTKRVLCPLV 516
           D+FGLT+PRATRLVAVPT+RVLCPLV
Sbjct: 481 DIFGLTSPRATRLVAVPTQRVLCPLV 506

BLAST of Spg012569 vs. ExPASy TrEMBL
Match: A0A6J1HDA0 (cytochrome P450 84A1 OS=Cucurbita moschata OX=3662 GN=LOC111463105 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.1e-259
Identity = 437/506 (86.36%), Postives = 471/506 (93.08%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGIF 69
           M L FT+PLLFLLA +F  RRKLPYPPGP+GLPF+GNMLMMD LTHRGLA LAKRYGGIF
Sbjct: 1   MALIFTIPLLFLLALIFWLRRKLPYPPGPRGLPFLGNMLMMDQLTHRGLAKLAKRYGGIF 60

Query: 70  HMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQM 129
           HMRMGFLHMV +S+PD+ARQVLQVHDNICSNRP TIAISYLTY++ADMAFAHYGPFWRQM
Sbjct: 61  HMRMGFLHMVTISDPDMARQVLQVHDNICSNRPVTIAISYLTYNQADMAFAHYGPFWRQM 120

Query: 130 RKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAAFGT 189
           RK+CVMKLFSRKRAESWASVRDEVD+ +R V  + GT VN+GE VFELT +IIYRAAFGT
Sbjct: 121 RKLCVMKLFSRKRAESWASVRDEVDSTVRHVATQLGTVVNVGERVFELTMNIIYRAAFGT 180

Query: 190 SAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLDKFIDTIID 249
           S VQGQDEFI+ILQEFS+LFGAFNIADFIP+L WIDPQGLNNRLVKARQSLDKFIDTIID
Sbjct: 181 STVQGQDEFIKILQEFSKLFGAFNIADFIPNLSWIDPQGLNNRLVKARQSLDKFIDTIID 240

Query: 250 EHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRDNIKAIIMD 309
           EHME KR R LSNVKDDEI+TDMVDEL+AFYDE  +VNE +D QN IKLNRDNIKAIIMD
Sbjct: 241 EHMEKKRIRKLSNVKDDEIETDMVDELVAFYDEQVSVNELDDSQNGIKLNRDNIKAIIMD 300

Query: 310 VMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTFLKCCLK 369
           VMFGGTETVAS IEW +SELMR+P+DLKKAQQELA+VVG  RRVEE+D ENL FLKCC+K
Sbjct: 301 VMFGGTETVASVIEWTLSELMRSPDDLKKAQQELADVVGLNRRVEESDMENLAFLKCCIK 360

Query: 370 ETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFLE 429
           ETLRLHPPIPLLLHETAED VV+GYFIPAKSRV+VNAWAIGRDPASWDDAETFRPARFL+
Sbjct: 361 ETLRLHPPIPLLLHETAEDAVVAGYFIPAKSRVIVNAWAIGRDPASWDDAETFRPARFLK 420

Query: 430 QGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPSEMDMG 489
           QGV DFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKP+EMDMG
Sbjct: 421 QGVADFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPNEMDMG 480

Query: 490 DVFGLTAPRATRLVAVPTKRVLCPLV 516
           D+FGLTAPRATRLVA+PTKRVLCPLV
Sbjct: 481 DIFGLTAPRATRLVALPTKRVLCPLV 506

BLAST of Spg012569 vs. ExPASy TrEMBL
Match: A0A0A0KKL4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504720 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.1e-247
Identity = 428/518 (82.63%), Postives = 469/518 (90.54%), Query Frame = 0

Query: 3   SLLQPLHMPLFFTLPLLFLLAFLF--RFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLAN 62
           SLLQPL+M +FFTLP L LL  L   R R KLP+PPGPKGLPFIGNMLM+D LTHRGLA 
Sbjct: 5   SLLQPLNMAVFFTLPFLVLLTLLIFRRLRPKLPFPPGPKGLPFIGNMLMLDQLTHRGLAK 64

Query: 63  LAKRYGGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFA 122
           LA RYGGIF+MRMGFL+M  V++PD+ARQVLQVHD+ICSNRPATIAISYLTY RADMAFA
Sbjct: 65  LAARYGGIFYMRMGFLNMFTVADPDIARQVLQVHDSICSNRPATIAISYLTYSRADMAFA 124

Query: 123 HYGPFWRQMRKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRD 182
           +YGPFWRQMRK+CVMKLFSRKRAESW SVRD VD  ++KV  +TGTAVN+GELVFELTRD
Sbjct: 125 NYGPFWRQMRKICVMKLFSRKRAESWQSVRDVVDNTVKKVAVQTGTAVNVGELVFELTRD 184

Query: 183 IIYRAAFGTSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSL 242
           IIYRAAFGTS +QGQDEFI ILQEFS+LFGAFNIADFIP L WIDPQGLN RL++ARQSL
Sbjct: 185 IIYRAAFGTSTLQGQDEFISILQEFSKLFGAFNIADFIPGLRWIDPQGLNKRLIEARQSL 244

Query: 243 DKFIDTIIDEHMENKRKRT--LSNVKDDEIDTDMVDELLAFY-DEHATVNESNDQQNTIK 302
           DKFID+IIDEH+  KR  +  LSN K  E ++DMVDELLAFY D+ + +NESND QN IK
Sbjct: 245 DKFIDSIIDEHIMKKRNASSKLSNYK-LETESDMVDELLAFYSDDSSIINESNDIQNNIK 304

Query: 303 LNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETD 362
           LNRDNIK IIMDVMFGGTETVASAIEWAMSELMR+PEDLKKAQQEL++VVG +RRVEETD
Sbjct: 305 LNRDNIKGIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELSQVVGLQRRVEETD 364

Query: 363 FENLTFLKCCLKETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWD 422
            +NLTFLKCCLKET+RLHPPIPLLLHE AED V++GYFIPAKSR+MVNAWAIGRDPASWD
Sbjct: 365 LDNLTFLKCCLKETIRLHPPIPLLLHEAAEDAVIAGYFIPAKSRIMVNAWAIGRDPASWD 424

Query: 423 DAETFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWEL 482
           DAETFRPARFLE+GVPDFKG+NFEFIPFGSGRRSCPGMQLGLYGLE+AVAHLLHCF WEL
Sbjct: 425 DAETFRPARFLEEGVPDFKGNNFEFIPFGSGRRSCPGMQLGLYGLEIAVAHLLHCFDWEL 484

Query: 483 PDGMKPSEMDMGDVFGLTAPRATRLVAVPTKRVLCPLV 516
           PDGMKPSEMDM DVFGLTAPRATRLVAVPTKRVLCPL+
Sbjct: 485 PDGMKPSEMDMSDVFGLTAPRATRLVAVPTKRVLCPLL 521

BLAST of Spg012569 vs. ExPASy TrEMBL
Match: A0A5A7SJ62 (Cytochrome P450 84A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001510 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.6e-244
Identity = 425/517 (82.21%), Postives = 467/517 (90.33%), Query Frame = 0

Query: 3   SLLQPLHMPLFFTLPLLFLLAFLF-RFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANL 62
           SLLQPL+M + FTL  LFLL  LF R R KLP+PPGPKGLPFIGNMLMM+ LTHRGLANL
Sbjct: 5   SLLQPLNMAVPFTLSFLFLLTLLFRRLRSKLPFPPGPKGLPFIGNMLMMEQLTHRGLANL 64

Query: 63  AKRYGGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAH 122
           A RYGGIF+MRMGF++M  VSNPD+ARQVLQVHD+ICSNRPATIAISYLTY+ ADMAFA+
Sbjct: 65  AARYGGIFYMRMGFVNMFVVSNPDIARQVLQVHDSICSNRPATIAISYLTYNCADMAFAN 124

Query: 123 YGPFWRQMRKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDI 182
           YGPFWRQMRK+CVMKLFSRKRAESW SVRD VD  ++KV  +TGTAVNIGELVFEL  DI
Sbjct: 125 YGPFWRQMRKICVMKLFSRKRAESWQSVRDMVDNTVKKVAVQTGTAVNIGELVFELMGDI 184

Query: 183 IYRAAFGTSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLD 242
           IYRAAFGTS +QGQDEFIRILQEFS+LFGAFNIADFIPSL WIDPQGLN RLVKARQSLD
Sbjct: 185 IYRAAFGTSTLQGQDEFIRILQEFSKLFGAFNIADFIPSLRWIDPQGLNKRLVKARQSLD 244

Query: 243 KFIDTIIDEHMENKRKRTLSNVKDDEIDT--DMVDELLAFYDEHATV-NESNDQQNTIKL 302
           KFID+IID+H+  KR  + S + + E++T  DMVDELLAFY E +++ N S+D +N IKL
Sbjct: 245 KFIDSIIDDHIIRKRNAS-SKLPNYELETESDMVDELLAFYGEDSSIRNASDDLKNNIKL 304

Query: 303 NRDNIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDF 362
           NRDNIK IIMDVMFGGTETVASAIEWAMSELMR+PEDLKKAQQEL++VVG +RRVEETD 
Sbjct: 305 NRDNIKGIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELSQVVGLQRRVEETDL 364

Query: 363 ENLTFLKCCLKETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDD 422
           +NLTFLKCCLKET+RLHPPIPLLLHE AED V++GYFIPAKSR+MVNAWAIGRDPASWDD
Sbjct: 365 DNLTFLKCCLKETIRLHPPIPLLLHEAAEDAVIAGYFIPAKSRIMVNAWAIGRDPASWDD 424

Query: 423 AETFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELP 482
           AETFRP RFLEQGVPDFKG+NFEFIPFGSGRRSCPGMQLGLYGLE+AVA LLHCF WELP
Sbjct: 425 AETFRPTRFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLGLYGLEIAVAQLLHCFDWELP 484

Query: 483 DGMKPSEMDMGDVFGLTAPRATRLVAVPTKRVLCPLV 516
           DGMKPSEMDM DVFGLTAPRATRLVAVPTKRVLCPL+
Sbjct: 485 DGMKPSEMDMSDVFGLTAPRATRLVAVPTKRVLCPLL 520

BLAST of Spg012569 vs. ExPASy TrEMBL
Match: A0A1S3B201 (cytochrome P450 84A1-like OS=Cucumis melo OX=3656 GN=LOC103485107 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.6e-244
Identity = 425/517 (82.21%), Postives = 467/517 (90.33%), Query Frame = 0

Query: 3   SLLQPLHMPLFFTLPLLFLLAFLF-RFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANL 62
           SLLQPL+M + FTL  LFLL  LF R R KLP+PPGPKGLPFIGNMLMM+ LTHRGLANL
Sbjct: 5   SLLQPLNMAVPFTLSFLFLLTLLFRRLRSKLPFPPGPKGLPFIGNMLMMEQLTHRGLANL 64

Query: 63  AKRYGGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAH 122
           A RYGGIF+MRMGF++M  VSNPD+ARQVLQVHD+ICSNRPATIAISYLTY+ ADMAFA+
Sbjct: 65  AARYGGIFYMRMGFVNMFVVSNPDIARQVLQVHDSICSNRPATIAISYLTYNCADMAFAN 124

Query: 123 YGPFWRQMRKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDI 182
           YGPFWRQMRK+CVMKLFSRKRAESW SVRD VD  ++KV  +TGTAVNIGELVFEL  DI
Sbjct: 125 YGPFWRQMRKICVMKLFSRKRAESWQSVRDMVDNTVKKVAVQTGTAVNIGELVFELMGDI 184

Query: 183 IYRAAFGTSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLD 242
           IYRAAFGTS +QGQDEFIRILQEFS+LFGAFNIADFIPSL WIDPQGLN RLVKARQSLD
Sbjct: 185 IYRAAFGTSTLQGQDEFIRILQEFSKLFGAFNIADFIPSLRWIDPQGLNKRLVKARQSLD 244

Query: 243 KFIDTIIDEHMENKRKRTLSNVKDDEIDT--DMVDELLAFYDEHATV-NESNDQQNTIKL 302
           KFID+IID+H+  KR  + S + + E++T  DMVDELLAFY E +++ N S+D +N IKL
Sbjct: 245 KFIDSIIDDHIIRKRNAS-SKLPNYELETESDMVDELLAFYGEDSSIRNASDDLKNNIKL 304

Query: 303 NRDNIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDF 362
           NRDNIK IIMDVMFGGTETVASAIEWAMSELMR+PEDLKKAQQEL++VVG +RRVEETD 
Sbjct: 305 NRDNIKGIIMDVMFGGTETVASAIEWAMSELMRSPEDLKKAQQELSQVVGLQRRVEETDL 364

Query: 363 ENLTFLKCCLKETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDD 422
           +NLTFLKCCLKET+RLHPPIPLLLHE AED V++GYFIPAKSR+MVNAWAIGRDPASWDD
Sbjct: 365 DNLTFLKCCLKETIRLHPPIPLLLHEAAEDAVIAGYFIPAKSRIMVNAWAIGRDPASWDD 424

Query: 423 AETFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELP 482
           AETFRP RFLEQGVPDFKG+NFEFIPFGSGRRSCPGMQLGLYGLE+AVA LLHCF WELP
Sbjct: 425 AETFRPTRFLEQGVPDFKGNNFEFIPFGSGRRSCPGMQLGLYGLEIAVAQLLHCFDWELP 484

Query: 483 DGMKPSEMDMGDVFGLTAPRATRLVAVPTKRVLCPLV 516
           DGMKPSEMDM DVFGLTAPRATRLVAVPTKRVLCPL+
Sbjct: 485 DGMKPSEMDMSDVFGLTAPRATRLVAVPTKRVLCPLL 520

BLAST of Spg012569 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 775.0 bits (2000), Expect = 3.7e-224
Identity = 378/505 (74.85%), Postives = 434/505 (85.94%), Query Frame = 0

Query: 12  LFFTLPLLFLLAFLFRFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYGGIFHM 71
           L   + L   ++F+ R RR+ PYPPGP+G P IGNMLMMD LTHRGLANLAK+YGG+ H+
Sbjct: 19  LVIVVSLFIFISFITR-RRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLCHL 78

Query: 72  RMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFWRQMRK 131
           RMGFLHM AVS+P+VARQVLQV D++ SNRPATIAISYLTYDRADMAFAHYGPFWRQMRK
Sbjct: 79  RMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMRK 138

Query: 132 VCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAAFGTSA 191
           VCVMK+FSRKRAESWASVRDEVD  +R V+   G  +N+GE +F LTR+I YRAAFG++ 
Sbjct: 139 VCVMKVFSRKRAESWASVRDEVDKMVRSVSCNVGKPINVGEQIFALTRNITYRAAFGSAC 198

Query: 192 VQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKARQSLDKFIDTIIDEH 251
            +GQDEFIRILQEFS+LFGAFN+ADFIP  GWIDPQG+N RLVKAR  LD FID IIDEH
Sbjct: 199 EKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDGFIDDIIDEH 258

Query: 252 MENKRKRTLSNVKD-DEIDTDMVDELLAFYDEHA-TVNESNDQQNTIKLNRDNIKAIIMD 311
           M  K+K   + V D D +DTDMVD+LLAFY E A  V+E+ D QN+IKL RDNIKAIIMD
Sbjct: 259 M--KKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRDNIKAIIMD 318

Query: 312 VMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTFLKCCLK 371
           VMFGGTETVASAIEWA++EL+R+PEDLK+ QQELAEVVG +RRVEE+D E LT+LKC LK
Sbjct: 319 VMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLK 378

Query: 372 ETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAETFRPARFLE 431
           ETLR+HPPIPLLLHETAED  + G+FIP KSRVM+NA+AIGRDP SW D +TFRP+RFLE
Sbjct: 379 ETLRMHPPIPLLLHETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLE 438

Query: 432 QGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGMKPSEMDMG 491
            GVPDFKGSNFEFIPFGSGRRSCPGMQLGLY L++AVAH+LHCFTW+LPDGMKPSE+DM 
Sbjct: 439 PGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMN 498

Query: 492 DVFGLTAPRATRLVAVPTKRVLCPL 515
           DVFGLTAP+ATRL AVPT R++C L
Sbjct: 499 DVFGLTAPKATRLFAVPTTRLICAL 520

BLAST of Spg012569 vs. TAIR 10
Match: AT5G04330.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 630.9 bits (1626), Expect = 8.7e-181
Identity = 314/515 (60.97%), Postives = 388/515 (75.34%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLFRFRRKL---PYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRYG 69
           M L   +PLL  L      RR++   PYPPGPKGLP IGN+LMM+   HRGLA L++ YG
Sbjct: 5   MTLIVLVPLLLFLFPHLLLRRQMLLKPYPPGPKGLPVIGNILMMNQFNHRGLAKLSRIYG 64

Query: 70  GIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPFW 129
           G+ H+R+GF H+  VS+PD+ARQVLQV D++ SNRP TIAI YLTY  +D+AF +YGPFW
Sbjct: 65  GLLHLRLGFSHIFVVSSPDIARQVLQVQDHVFSNRPTTIAIRYLTYGGSDLAFCNYGPFW 124

Query: 130 RQMRKVCVMKLFSRKRAESWASVRDEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYRAA 189
           R+MRK+ VM LFSRKRAESW SV +EV  ++R V    G  +NI +L F L+RDI +RAA
Sbjct: 125 RRMRKLYVMMLFSRKRAESWVSVDEEVHKSVRLVASNVGKPLNICKLAFSLSRDITFRAA 184

Query: 190 FGTSAVQGQ----DEFIRILQEFSRLFGAFNIADFIPS-LGWIDPQGLNNRLVKARQSLD 249
           FG+S+        DEF+ I+QEFS+LFG FN+AD++PS L WIDPQG+N R+ KAR+SLD
Sbjct: 185 FGSSSSTSDESRLDEFLEIIQEFSKLFGEFNVADYVPSWLSWIDPQGINGRVEKARKSLD 244

Query: 250 KFIDTIIDEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRD 309
            FI+++ID+H+ +K+KR   NV +   +TDMVD+LLAFY+E   VN S       K+N D
Sbjct: 245 GFIESVIDDHL-HKKKREHDNVDE---ETDMVDQLLAFYEEEVKVNNS-----VTKINLD 304

Query: 310 NIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFER-RVEETDFEN 369
           NIK IIMDVMFGGTETVA AIEW ++E++R+PE++K+ Q EL  VVG +R RVE+T  E 
Sbjct: 305 NIKGIIMDVMFGGTETVALAIEWVLTEILRSPENMKRVQDELTSVVGLDRWRVEDTHLEK 364

Query: 370 LTFLKCCLKETLRLHPPIPLLLHETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASWDDAE 429
           LTFLKC LKETLRLHPP PLLLHET +D  +SGYFIP  SRVMVN +A+GRDP SW D E
Sbjct: 365 LTFLKCILKETLRLHPPFPLLLHETVKDTEISGYFIPKGSRVMVNTYALGRDPNSWSDPE 424

Query: 430 TFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDG 489
           +F P RFL    PD KG+NFEF+PFGSGRRSCPGMQLGLY  E+AVAHLLHCFTW LPDG
Sbjct: 425 SFNPGRFLNPIAPDLKGNNFEFVPFGSGRRSCPGMQLGLYAFELAVAHLLHCFTWSLPDG 484

Query: 490 MKPSEMDMGDVFGLTAPRATRLVAVPTKRVLCPLV 516
           M P ++D  +  GLT P+A  LVAVPT R+LCP+V
Sbjct: 485 MNPGDVDTVEGPGLTVPKAIPLVAVPTTRLLCPIV 510

BLAST of Spg012569 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 348.2 bits (892), Expect = 1.1e-95
Identity = 197/519 (37.96%), Postives = 292/519 (56.26%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLFRFRRKLPYP-----PGPKGLPFIGNMLMMDHLTHRGLANLAKR 69
           M   + LPL+FL+  L        +P     P P G P IGN+  +  L H+ L  L+K+
Sbjct: 1   MAHIWLLPLIFLVCILLAVFNHKKHPKYRQFPCPPGFPIIGNLHQIGELPHQTLWKLSKK 60

Query: 70  YGGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGP 129
           YG + H+ +G +  V VS+ D ARQVL+VHD  C  RP+      L+Y+  D+AF+ Y  
Sbjct: 61  YGPVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDD 120

Query: 130 FWRQMRKVCVMKLFSRKRAESWASVRDE-----VDTAIRKVTGRTGTAVNIGELVFELTR 189
           +W+++RK+CV +LFS K+  S   ++DE     +D+     + +    VN+     ELT 
Sbjct: 121 YWKEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQK--NPVNLNNKCLELTV 180

Query: 190 DIIYRAAFGTS---AVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDP--QGLNNRLV 249
            ++ R AFG S    V   D F +I++E   + G+F+ ADFIP +GWI     GL  R  
Sbjct: 181 SVVCRTAFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRE 240

Query: 250 KARQSLDKFIDTIIDEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQN 309
           ++++ L+ F + + D H E K+          E + D VD LL    E A +   ND   
Sbjct: 241 RSKRDLNAFFEQMFDLHKEGKK----------EGNEDFVDLLLRLEKEEAVL--GND--- 300

Query: 310 TIKLNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVE 369
             KL R++IKAI++DV+  G +T A  + WAM+EL RNP  +KK Q E+   +G    + 
Sbjct: 301 --KLTRNHIKAILLDVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMIS 360

Query: 370 ETDFENLTFLKCCLKETLRLHPPIPLLL-HETAEDLVVSGYFIPAKSRVMVNAWAIGRDP 429
             D + L +LK  +KET RLHP  PLLL  E   +  ++GY IP K+R+ VN WAIGRDP
Sbjct: 361 FEDMDQLEYLKMVIKETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDP 420

Query: 430 ASWDDAETFRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCF 489
            +W D E F P RF++  + D KG +FE +PFG GRR CP + +G   +E  +A+LL+ F
Sbjct: 421 DTWKDPEVFLPERFMDNNI-DAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHF 480

Query: 490 TWELPDGMKPSEMDMGDVFGLTAPRATRLVAVPTKRVLC 513
            W+LP+G++  ++D+ +  GLT  +   L+ VP  R  C
Sbjct: 481 DWKLPEGVEVKDIDVEEAPGLTVNKKNELLLVPEMRRSC 499

BLAST of Spg012569 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 342.8 bits (878), Expect = 4.7e-94
Identity = 182/504 (36.11%), Postives = 296/504 (58.73%), Query Frame = 0

Query: 10  MPLFFTLPLLFLLAFLF----RFRRKLPYPPGPKGLPFIGNMLMMDHLTHRGLANLAKRY 69
           M +     L+  +  LF    +  +K   P  P  LP IGN+  + H  HR L +L+ RY
Sbjct: 4   MTMIILQSLIIFITILFFKKQKRGKKSNTPRSPPRLPLIGNLHQLGHHPHRSLCSLSHRY 63

Query: 70  GGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGPF 129
           G +  + +G + ++ VS+ DVAR +L+ HD + ++RP +     L YD  D+AFA YG +
Sbjct: 64  GPLMLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGEY 123

Query: 130 WRQMRKVCVMKLFSRKRAESWASVR-DEVDTAIRKVTGRTGTAVNIGELVFELTRDIIYR 189
           WRQ++ VCV++L S K   S+ +VR +E+   + K+   +   VN+ EL+  LT D+I R
Sbjct: 124 WRQIKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSLQVNVSELLGSLTNDVISR 183

Query: 190 AAFGTSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWID-PQGLNNRLVKARQSLDKF 249
            A G     G+ +   +++    L G F++  ++P LGWID   GL+ +L K    LD+F
Sbjct: 184 IALGRK-YSGETDSKELMKRLMMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLDEF 243

Query: 250 IDTIIDEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIKLNRDNI 309
           ++ ++ +H++   +R           TD VD LL    E +            +++R  I
Sbjct: 244 LEKVVQDHVDGDGQR-----------TDFVDVLLRIQREKSI---------GFEIDRLCI 303

Query: 310 KAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETDFENLTF 369
           KAI++DV+  GT++  + ++WAM+EL+R+PE L+  Q+E+  +      V E D +N+++
Sbjct: 304 KAIVLDVLVAGTDSSYALMDWAMTELLRHPECLRTLQEEVRTICKGNLSVSEEDIQNMSY 363

Query: 370 LKCCLKETLRLHPPIPLLL-HETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASW-DDAET 429
           LK  +KET RLHPP+PLL  HE+ +D+++  Y IPA ++VM+NAWAIGR+ A+W  DAE 
Sbjct: 364 LKAVIKETTRLHPPLPLLAPHESIQDVILGDYHIPAGTQVMINAWAIGREAATWGPDAEK 423

Query: 430 FRPARFLEQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCFTWELPDGM 489
           FRP R L+  V DF+G NFE +PFG+GRR CP +   +  +E+A+A+ +H + W+LP+  
Sbjct: 424 FRPERHLDSSV-DFRGHNFELVPFGAGRRICPAISFAVVLIEVALANFVHRYDWKLPEDS 483

Query: 490 KPSEMDMGDVFGLTAPRATRLVAV 506
           K ++ ++ +  G+   R   L A+
Sbjct: 484 KENQTNVAESTGMVIHRLFPLYAI 485

BLAST of Spg012569 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 340.5 bits (872), Expect = 2.4e-93
Identity = 200/517 (38.68%), Postives = 293/517 (56.67%), Query Frame = 0

Query: 12  LFFTLPL---LFLLAFLFRFRRKLPY----PPGPKGLPFIGNMLMMDHLTHRGLANLAKR 71
           LF T+ L   LFL+  +F  RR   +    PPGP   P IGN+  M    HR L+ +   
Sbjct: 4   LFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTT 63

Query: 72  YGGIFHMRMGFLHMVAVSNPDVARQVLQVHDNICSNRPATIAISYLTYDRADMAFAHYGP 131
           YG I H+R+GF+ +V  ++  VA Q L++HD   ++RP      ++ Y+  D+ FA YG 
Sbjct: 64  YGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGH 123

Query: 132 FWRQMRKVCVMKLFSRKRAESWASVR-DEVDTAIRKVTGRTGT-AVNIGEL-----VFEL 191
            WR +RK+  + LFS K  E +  VR +EV T  R++  R GT  VN+G+L     V  L
Sbjct: 124 RWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELV-RVGTKPVNLGQLVNMCVVNAL 183

Query: 192 TRDIIYRAAFGTSAVQGQDEFIRILQEFSRLFGAFNIADFIPSLGWIDPQGLNNRLVKAR 251
            R++I R  FG  A    DEF  ++ E   L G FNI DF+PSL W+D QG+  ++ +  
Sbjct: 184 GREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLH 243

Query: 252 QSLDKFIDTIIDEHMENKRKRTLSNVKDDEIDTDMVDELLAFYDEHATVNESNDQQNTIK 311
           +  D F+ +I+ EH  N +         D+  TDM+  L+       ++  ++   +   
Sbjct: 244 KRFDAFLSSILKEHEMNGQ---------DQKHTDMLSTLI-------SLKGTDLDGDGGS 303

Query: 312 LNRDNIKAIIMDVMFGGTETVASAIEWAMSELMRNPEDLKKAQQELAEVVGFERRVEETD 371
           L    IKA+++++   GT+T AS ++WA++EL+R+P+ + KAQ+EL  VVG +R V E+D
Sbjct: 304 LTDTEIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESD 363

Query: 372 FENLTFLKCCLKETLRLHPPIPLLL-HETAEDLVVSGYFIPAKSRVMVNAWAIGRDPASW 431
              L +L+  +KE  RLHPP PL L H  +E   ++GY IP  S ++ N WAI RDP  W
Sbjct: 364 IAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQW 423

Query: 432 DDAETFRPARFL---EQGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLEMAVAHLLHCF 491
            D   F+P RFL   E+   D KGS+FE IPFG+GRR C G+ LGL  ++   A L+  F
Sbjct: 424 SDPLAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGF 483

Query: 492 TWELPDGMKPSEMDMGDVFGLTAPRATRLVAVPTKRV 511
            WEL  G+ P +++M + +GLT  RA  LV  P  R+
Sbjct: 484 DWELAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRL 503