Spg012491 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012491
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationscaffold1: 15539011 .. 15543362 (-)
RNA-Seq ExpressionSpg012491
SyntenySpg012491
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCGGCGGCCCGCCGCACACCTGGCCGGAACCGGTGGTCCGAGTCCAATCCCTAACCCGAACCGGCCTCCACACAATCCCAGACCGGTACATCAGACCGCCGTCGTCGAGGCCCTCCGCCGCCGACGATTCGATCGAAATCCCGGCCGTGGACTTCGGCGAGCTGTTCGGCGGCGACGAGAGCCGCCGTGAGGCGAGCCGAAGACAGGTATCGGAGGCTTGTAGAGAGTGGGGGATTTTTCAGATGATAAACCACGGAATCGAGACGGAGGTGATGAAGAAGGTGAGAGATGTGTGGCGGAGGTTCTTCGAATCGCCGGCGGAGGAGAATTAGGAGGAATACGCGAACTCGCCGAGATCGTATGAAGGATACGGCAGCCGGATGGGAGTCCAAGAAGGAGCGGTGTTGGATTGGAGTGATTATTTTTTTCTTCATTTTTCGCCGCCGTCTCACCGGAACCCTAACAAGTTGCCTGCCATTCCTCCTTCCTGCAGGTAAATTATTATAACAAACAAACAACAAAAAAAAAAAGTATGTATATATACACACATACACGTATGTTTGGGAGTGATTTTAAAATTATTAAAATCACTTTTTCTATATTTAAAATCATTCTAAAACATGTCTTCTAAAAGTGTAAAAATCAATCATGAAATTGAATTGGAATAATTAAAAAGGTATGTTCTAAGGTGATTTCGAACATAACAAAAATGATTTTAGTCATTTCAAAATCACTCCCAATCATACACTATATATATAATCTCTTGACCAAGGATATCAGCAGATGTTTTATACTGTTAAAGCTATACTAAAACAAGAACATGACTCTTGTTTAACAACCTTTTTTAAAAAAGCATAGGAAAAAATCATTTTTTACCCTAAACTTGACTTGTATCAATTTAAATCTTGAACTTTCAATTTCATTAAATTGAACCTTAAACTTAATAAGTTATATCAATTATAACCCTAAACTTTTAATTTCATCAAATCGACCTCAAACTAAATAAATTGTATCAATTTTAACTTCAAATTTACGTAAGTTTTGCAATGTAAACCTTCAATTATATTTATGTTTGAACCGTTGTTAAAGCATGTAATTGACGACTATTTCCTTTAAAAAAAAAATTATATTGCACCTGAATTGGTTTCTTGTAAAAAGAAAATATTGGAGAAAATTTTTATTTAAAGATACTTTGCTACCGGGAAGTTGTACATGAGTTTTCTCTCTTTTTTTTTTCCTTATTATGTTTGTGAGATTTATTGCCTTTTTATTAGCTACTTAGAAAAACATGCAAAGATGATAGATAGCTACTTAGAAAAACATGCAAAGATGATAGAAAATCAATTCTGAGATTTTTTTTTTTCTTTTCATTTTAAATGACTTTTTCTTGTTTATTTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTATCTAGGATTACGAAAGCAAAATGATTTTGAATGTTTTTTTTTTCCTTCAAATGTTTGTTGTTGCTTTTTTTTTCTAAGAATTTTGTGGGGTTTTTTTTGTCTTCTTTTGTTTTTTTTCTCCTACTAATTAGAAGAACATGCAAATGTAGGAAAAGAAAGCAAAACAATTTTGTGTTTTTTTTCCCTCATATTTGCTAGATTTTTCTTGTTGCTAATAACTTTGTAAGTTTTTTTTAGGAAGAATACTAATAATGACGAGAAGAATCACTCTAGAGTATTTGATCTCTCCCAATAAAGAGTTAACAAATTTCTTACAAGAAACCAACTCAGGTGCAATGTAGATTTTTTTAAAAAAAAAAAAAAAAAAAAAGAAATTGTAAATCGCATGCTTTAATGATAATTTGAGTGGAAAGTTAACCGAAGGTCTACATTGCAACACTTATGCAAGTTTAAGGTTAAAAATTGATATAATATGCTAAGTTTGGGGTTCAATTGGATGAAACTGAAAGTTCAGGGTTAAAATCGATACAACATGCTAAGTTTAGGGTTATAATTGATTTTTTTTCCAAGAAACATATTTAGTATCTGTATTTTTATATAGTATATATGCAAAGAGAGTGCCAACAAGCACATAGTTCAACTAACACAAGTTCGATAAAAAGGTCAAAAGTTCAAATTTCCCACCCCATGTCGTAAAAAAGAAAATATGCAAAGAGAGTTGATTCTTTGGACTGAACGCAAAGAATGTAAGTAAAGGTATGGGAATTTTAATCTCGAACCAGCGTTGATTTTGGAGGAGGTTGAAGTGATTAAAATAGAATCTTAACCATTTTTAGCGTTTGGTAAAATAGATTATAAATTAATTTTTATAAAAGTACTTTTGTAACTAACTGTTTATCTTCTCTACACGTTTTTTTAGACTTGATTCATGTCAATCATTTTTTTAATTTAAGTTAAATATTTTTATTCTATTATCTAATCTTATACTCAATTGAAATTATTTGTTTTTATTTTTATTTAAATTGAAATAATATTTTTTTAATACAAGTAGCAAATGATATTTCTTAGCCAAATATAAAAACATCCTAGATTAAACATAACATTATATTTTAAATCATATGAAATTTAAAACAATTATCTTATATGTTTCAAAAAAAAATAATTGTCTTAAAATAATTTTGAAATTATCTTATAAAACTTTGAAATTAAATTTCAACATTAAATTTATTTATTGAAAAAATATAGTTAAGTTGCAATAATTTAGAATATTAATTTACCAAGCACCTCAACTTGCTTTTTCATCTATAAAACTAAAATTTACGAAATACTAATTTACTTTATGGTACAATTGATTTTCTTAGAAGTACAGTCAACAACAATTATTTTAGAAGCTACAACAATCCTAAACTAAGCCTAATGCTTTAACTAGGATGTAAATTCATATTTGTTTTTATTTTAAAATTTGTTACAATATTATTTTAGTTGTTGTAGTTGCACATGTTCAAATTTATCTATTTCATGTACTTTTAATAAATCTTAAAAGTTAAACATCAATTATCAATTTTTCATCCTTAATTTATGATTAACTTTTGTTTTAAAGAAAGTAAAGTCATTAAAATATATATATTAAAAAAAAAAATCTTATTACTCTCTATAAGCCTTCCTTTGATAAGCATGAATAAATTATGTGCTTTATTAAAGGGAGATTATTATTATTCTTTTGTAAAGTCAAGTTAACTTCAATTGTTAATAAACCATTGTGAGAGAAACACTTGAAACCAACTTTTTCAAAATGAGTTTTTAAGTATACAATAACTTGCGTATAAAAACACTTATGAGGAAATGTAACCAAACATATAAGTTTTTAAATTCTAAACAATTTTTTTGCAAAATGTTTTTTAAAAAATGCATTCTTGAAAAACATTTAAATTAAAATGTATTTCAAACCGACCTTTAGTCTCTAATGAAAATTTTCATTTTAATTATCTTTTCAATAACTTTAAAAGTTTTATTGTCATTTCTATCCACACCGATTATAGTTTACTGTCTTGGTTAACTAGGGCAAAAATCTCACGCATTAGATATAGGCATAATAGATAGAGCTTTGTGGTGTAATATTTTGTTTTAGTGATATGAATAAACATGCTACACCTTTATAAATATATATATATATATATATATATATATATATGGATATCAAGTCATAAAATATTATTTTGTTTGGAAAAAAACCGAATAAATACGGTGACTAGTGATGTTTTAGAGTGCAATCAGTCAATATTATATCAGTTGGATTTTTATTTCTTTTCTCTAAGGATCGAGATGTTAGGACCCATTTGATAATCGTACAGAGTGTGTGTGTGATTAATATTGCAGGATTATATTTGAACCTCTGATCCCTTGTCAAGCAACGTGGTTTTTGTTTGTGATTAATATTGCAGGAAGATAACAGAAGAATATGGAGAAGAAATGATGAAGTTAAGTAGAAAGATGATGAGGGTATTTTCGGAAAATCTTGGTTTAAGGGAAGACTACATTGAGAAAGCATTTGGAGAGGCAGGGATGGGGGGATGCCTTAGGGTTAATTTGTACCCAAAATGCCCTCAGCCAGAGCTGACTTTGGGAATATCATGTCATTCTGATCCTGGTGGCCTTACCATTCTGTTGGCTGATGAAAATGTGAGTGATGGACTCCAAGTTTCTAAGGCCAATGTTTGGGTCAATGTTAAGCCTCTTCCAAATGCCTTCATTCTCAATATTGGAGATCAAATTCAGGTCTCTCTCTCTCTCTTTCAACTTTCTACTCTCATTTTGGTTTTAATTCTCTAACTAATTTTGAGGAGCCTTATCCATTATTGAAGTCAATTGTTTAGGTCTCGTTTGATAACTATTTGGACCTTGTTTGA

mRNA sequence

ATGACCGGCGGCCCGCCGCACACCTGGCCGGAACCGGTGGTCCGAGTCCAATCCCTAACCCGAACCGGCCTCCACACAATCCCAGACCGGTACATCAGACCGCCGTCGTCGAGGCCCTCCGCCGCCGACGATTCGATCGAAATCCCGGCCGTGGACTTCGGCGAGCTGTTCGGCGGCGACGAGAGCCGCCGTGAGGCGAGCCGAAGACAGGTATCGGAGGCTTGTAGAGAGTGGGGGATTTTTCAGATGATAAACCACGGAATCGAGACGGAGGTGATGAAGAAGGTGAGAGATGTGTGGCGGAGCCGGATGGGAGTCCAAGAAGGAGCGGTGTTGGATTGGAGTGATTATTTTTTTCTTCATTTTTCGCCGCCGTCTCACCGGAACCCTAACAAGTTGCCTGCCATTCCTCCTTCCTGCAGGAAGATAACAGAAGAATATGGAGAAGAAATGATGAAGTTAAGTAGAAAGATGATGAGGGTATTTTCGGAAAATCTTGGTTTAAGGGAAGACTACATTGAGAAAGCATTTGGAGAGGCAGGGATGGGGGGATGCCTTAGGGTTAATTTGTACCCAAAATGCCCTCAGCCAGAGCTGACTTTGGGAATATCATGTCATTCTGATCCTGGTGGCCTTACCATTCTGTTGGCTGATGAAAATGTGAGTGATGGACTCCAAGTTTCTAAGGCCAATGTTTGGGTCAATGTTAAGCCTCTTCCAAATGCCTTCATTCTCAATATTGGAGATCAAATTCAGGTCTCGTTTGATAACTATTTGGACCTTGTTTGA

Coding sequence (CDS)

ATGACCGGCGGCCCGCCGCACACCTGGCCGGAACCGGTGGTCCGAGTCCAATCCCTAACCCGAACCGGCCTCCACACAATCCCAGACCGGTACATCAGACCGCCGTCGTCGAGGCCCTCCGCCGCCGACGATTCGATCGAAATCCCGGCCGTGGACTTCGGCGAGCTGTTCGGCGGCGACGAGAGCCGCCGTGAGGCGAGCCGAAGACAGGTATCGGAGGCTTGTAGAGAGTGGGGGATTTTTCAGATGATAAACCACGGAATCGAGACGGAGGTGATGAAGAAGGTGAGAGATGTGTGGCGGAGCCGGATGGGAGTCCAAGAAGGAGCGGTGTTGGATTGGAGTGATTATTTTTTTCTTCATTTTTCGCCGCCGTCTCACCGGAACCCTAACAAGTTGCCTGCCATTCCTCCTTCCTGCAGGAAGATAACAGAAGAATATGGAGAAGAAATGATGAAGTTAAGTAGAAAGATGATGAGGGTATTTTCGGAAAATCTTGGTTTAAGGGAAGACTACATTGAGAAAGCATTTGGAGAGGCAGGGATGGGGGGATGCCTTAGGGTTAATTTGTACCCAAAATGCCCTCAGCCAGAGCTGACTTTGGGAATATCATGTCATTCTGATCCTGGTGGCCTTACCATTCTGTTGGCTGATGAAAATGTGAGTGATGGACTCCAAGTTTCTAAGGCCAATGTTTGGGTCAATGTTAAGCCTCTTCCAAATGCCTTCATTCTCAATATTGGAGATCAAATTCAGGTCTCGTTTGATAACTATTTGGACCTTGTTTGA

Protein sequence

MTGGPPHTWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAADDSIEIPAVDFGELFGGDESRREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWRSRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKMMRVFSENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQVSFDNYLDLV
Homology
BLAST of Spg012491 vs. NCBI nr
Match: PON92241.1 (Isopenicillin N synthase [Trema orientale])

HSP 1 Score: 321.6 bits (823), Expect = 6.2e-84
Identity = 150/277 (54.15%), Postives = 194/277 (70.04%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRP-----SAADDSIEIPAVDFGELFGGDES 67
           TWPEP++RVQSL  +G+  +P RYI+PPS RP     S   ++I IP +   +LF  DE+
Sbjct: 6   TWPEPIIRVQSLAESGIQRLPHRYIKPPSERPQTRVNSQDHETINIPVISLEDLFARDET 65

Query: 68  RREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR--------------------- 127
            +EA+   +S ACREWG FQ++NHG+  E+MK+ RD WR                     
Sbjct: 66  LQEATLAAISSACREWGFFQVVNHGVSPELMKRARDTWREFFGQPLEEKQRYANSPSTYE 125

Query: 128 ---SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKM 187
              SR+GV++GAVLDWSDYFFLHF PPS RNP K P++PPSCR + EEYG E+++L   +
Sbjct: 126 GYGSRLGVEKGAVLDWSDYFFLHFLPPSLRNPEKWPSLPPSCRNVIEEYGNEVVRLCGDL 185

Query: 188 MRVFSENLGLREDYIEKAFG--EAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILL 247
           M++ S NLGL ED+++KAFG      G CLRVN YPKCPQP+LTLG+S HSDPGG+T+LL
Sbjct: 186 MKILSINLGLEEDHLQKAFGGENNNTGACLRVNFYPKCPQPDLTLGLSSHSDPGGMTLLL 245

Query: 248 ADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            DE+V+ GLQV + N W+ VKP+PNAFI+NIGDQIQV
Sbjct: 246 PDEDVA-GLQVRRDNSWITVKPVPNAFIVNIGDQIQV 281

BLAST of Spg012491 vs. NCBI nr
Match: PON51147.1 (Isopenicillin N synthase [Parasponia andersonii])

HSP 1 Score: 320.1 bits (819), Expect = 1.8e-83
Identity = 151/277 (54.51%), Postives = 193/277 (69.68%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRP-----SAADDSIEIPAVDFGELFGGDES 67
           TWPEP+VRVQSL  +G+  +P RYI+PPS RP     S   ++I IP +   +LF  DE+
Sbjct: 6   TWPEPIVRVQSLAESGIKRLPHRYIKPPSERPQTRVNSQDHETINIPVISLEDLFARDET 65

Query: 68  RREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR--------------------- 127
            +EA+   +S ACREWG FQ++NHG+  E+MK+ RD W                      
Sbjct: 66  LQEATLAAISSACREWGFFQVVNHGVSPELMKQARDTWSEFFGQPLEEKQRYANSPSTYE 125

Query: 128 ---SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKM 187
              SR+GV++GAVLDWSDYFFLHF PPS RNP K P++PPSCR + EEYG E+++L   +
Sbjct: 126 GYGSRLGVEKGAVLDWSDYFFLHFLPPSLRNPEKWPSLPPSCRNVIEEYGNEVVRLCGDL 185

Query: 188 MRVFSENLGLREDYIEKAFG--EAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILL 247
           M++ S NLGL ED+++KAFG      G CLRVN YPKCPQPELTLG+S HSDPGG+T+LL
Sbjct: 186 MKILSINLGLEEDHLQKAFGGENNNTGACLRVNFYPKCPQPELTLGLSSHSDPGGMTLLL 245

Query: 248 ADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            DE+V+ GLQV + N W+ VKP+PNAFI+NIGDQIQV
Sbjct: 246 PDEDVA-GLQVRRDNSWITVKPVPNAFIVNIGDQIQV 281

BLAST of Spg012491 vs. NCBI nr
Match: KAF3449533.1 (hypothetical protein FNV43_RR10262 [Rhamnella rubrinervis])

HSP 1 Score: 315.8 bits (808), Expect = 3.4e-82
Identity = 147/281 (52.31%), Postives = 195/281 (69.40%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRP-----------SAADDSIEIPAVDFGEL 67
           +WPEPVVRVQSL  +G+ +IP RYI+PPS RP           SA DD I IP +D  +L
Sbjct: 7   SWPEPVVRVQSLAESGISSIPKRYIKPPSQRPSSSSHNYNNLNSADDDDISIPVIDLQDL 66

Query: 68  FGGDESRREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR--------------- 127
           FG DES REA+   +S AC EWG FQ++NHG+  ++M +VR++WR               
Sbjct: 67  FGTDESLREAALACLSNACSEWGFFQVVNHGVSHQLMNQVREMWRGFFELPLEVKQEYSN 126

Query: 128 ---------SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMM 187
                    SR+GV +GA+LDWSDY+FL+F PP  R+P K P++PPSCR + EEYG E +
Sbjct: 127 SPTTYEGYGSRLGVDKGAILDWSDYYFLNFMPPELRSPEKWPSLPPSCRNLIEEYGNEAI 186

Query: 188 KLSRKMMRVFSENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGL 247
           +L  +++++ S  LGL EDY++ AFG   +G CLRVN YPKCPQP+LTLG+S HSDPGG+
Sbjct: 187 RLCGRLIKILSITLGLGEDYLQNAFGGDNIGACLRVNFYPKCPQPDLTLGLSPHSDPGGM 246

Query: 248 TILLADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
           T+LL DENV+ GLQV + + W+ VKP+PN+FI+NIGDQI+V
Sbjct: 247 TVLLPDENVA-GLQVRRGDSWITVKPIPNSFIVNIGDQIKV 286

BLAST of Spg012491 vs. NCBI nr
Match: XP_004302589.1 (PREDICTED: protein SRG1-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 314.3 bits (804), Expect = 9.9e-82
Identity = 145/271 (53.51%), Postives = 193/271 (71.22%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAADD-SIEIPAVDFGELFGGDESRREA 67
           +WPEP+VRVQSL ++G+  IP+ YI+PPS RPS  D+ ++ IP +D   L G D+  RE 
Sbjct: 7   SWPEPIVRVQSLAQSGMTKIPEHYIKPPSKRPSNVDNTTVNIPVIDMQSLTGDDQGLRET 66

Query: 68  SRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR------------------------S 127
           + + VS ACREWG FQ++NHG+  E+MK+ R+ WR                        S
Sbjct: 67  TLQLVSNACREWGFFQVVNHGVSHELMKRARETWREFFEQPLGVKQEYSNDPSTYEGYGS 126

Query: 128 RMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKMMRVF 187
           R+GV++GA+LDWSDY+FLH+ PP+ R P+K PA+P SCR + EEYGEE  KL  +++++ 
Sbjct: 127 RLGVEKGAILDWSDYYFLHYLPPALRKPHKWPALPSSCRDLIEEYGEEATKLCGRLVKML 186

Query: 188 SENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLADENVS 247
           S NLGL EDY+  AFG   +G CLRVN YP+CPQP+LTLGIS HSDPGG+T+LL DE+V+
Sbjct: 187 SLNLGLGEDYLPNAFGGENVGACLRVNFYPRCPQPDLTLGISPHSDPGGMTLLLPDEDVA 246

Query: 248 DGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            GLQV K + WV VKP+PNAFI+N+GDQ+QV
Sbjct: 247 -GLQVRKGDNWVTVKPVPNAFIVNMGDQMQV 276

BLAST of Spg012491 vs. NCBI nr
Match: XP_024191028.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Rosa chinensis] >PRQ42929.1 putative anthocyanidin synthase [Rosa chinensis])

HSP 1 Score: 313.2 bits (801), Expect = 2.2e-81
Identity = 148/275 (53.82%), Postives = 192/275 (69.82%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPS----AADDSIEIPAVDFGELFGGDESR 67
           +WPEP+VRVQSL ++G+ TIP+ YI+PP  RPS      D ++ IP +D   L G D+S 
Sbjct: 7   SWPEPIVRVQSLAQSGIKTIPELYIKPPCKRPSNDLVLDDATVNIPVIDLQALAGDDQSL 66

Query: 68  REASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR---------------------- 127
           RE + + VS ACREWG FQ++NHG+  E+MK+ R+ WR                      
Sbjct: 67  RETTLQLVSSACREWGFFQVVNHGVSHELMKRARETWREFFEQPLEVKQEYSNNPSTYEG 126

Query: 128 --SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKMM 187
             SR+GV++GA+LDWSDY+FLH+ PP  R P K PA+P SCR + EEYGEE  +L  ++M
Sbjct: 127 YGSRLGVEKGAILDWSDYYFLHYMPPELRKPTKWPALPSSCRNLIEEYGEEATRLCGRLM 186

Query: 188 RVFSENLGLREDYIEKAF-GEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLAD 247
           ++ S NLGL EDY+  AF GE  +G CLRVN YPKCPQP+LTLGIS HSDPGG+T+LL D
Sbjct: 187 KMLSLNLGLGEDYLLNAFGGEENVGACLRVNFYPKCPQPDLTLGISAHSDPGGMTLLLPD 246

Query: 248 ENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
           E+V+ GLQV K + WV VKP+PNAFI+N+GDQ+QV
Sbjct: 247 EDVA-GLQVRKGDSWVTVKPVPNAFIVNMGDQMQV 280

BLAST of Spg012491 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.2e-75
Identity = 136/277 (49.10%), Postives = 183/277 (66.06%), Query Frame = 0

Query: 4   GPPHTWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAADD---SIEIPAVDFGELFGGD 63
           G    WPEP+VRVQSL  + L ++PDRYI+P S RP+  +D   +  IP +D   LF  +
Sbjct: 15  GSMDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEE 74

Query: 64  ESRREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR------------------- 123
               +    ++SEACR WG FQ++NHG++ E+M   R+ WR                   
Sbjct: 75  GLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRT 134

Query: 124 -----SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSR 183
                SR+GV++GA LDWSDY+FLH  P   ++ NK P+ PP+ R++ +EYGEE++KLS 
Sbjct: 135 YEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSG 194

Query: 184 KMMRVFSENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILL 243
           ++MRV S NLGL+ED  ++AFG   +G CLRVN YPKCP+PEL LG+S HSDPGG+TILL
Sbjct: 195 RIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILL 254

Query: 244 ADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            D+ V  GLQV K + W+ VKP P+AFI+NIGDQIQ+
Sbjct: 255 PDDQVF-GLQVRKDDTWITVKPHPHAFIVNIGDQIQI 290

BLAST of Spg012491 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 3.5e-74
Identity = 131/276 (47.46%), Postives = 183/276 (66.30%), Query Frame = 0

Query: 9   WPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAA-------DDSIEIPAVDFGELFGGDE 68
           WPEP+VRVQSL  + L ++PDRYI+PPS RP             I IP +D   LF G+E
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 69  SRREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR-------------------- 128
                 ++++SEACREWG FQ+INHG++ E+M   R+ W+                    
Sbjct: 109 D----DKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTY 168

Query: 129 ----SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRK 188
               SR+GV++GA+LDW+DY++LHF P + ++ NK P++P + R++ +EYG+E++KL  +
Sbjct: 169 EGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGR 228

Query: 189 MMRVFSENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLA 248
           +M + S NLGLR + +++AFG   +G CLRVN YPKCPQPEL LG+S HSDPGG+TILL 
Sbjct: 229 LMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLP 288

Query: 249 DENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
           D+ V  GLQV   + W+ V PL +AFI+NIGDQIQ+
Sbjct: 289 DDQVV-GLQVRHGDTWITVNPLRHAFIVNIGDQIQI 319

BLAST of Spg012491 vs. ExPASy Swiss-Prot
Match: Q9LY48 (Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.2e-69
Identity = 126/276 (45.65%), Postives = 180/276 (65.22%), Query Frame = 0

Query: 9   WPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAA------DDSIEIPAVDFGELFGGDES 68
           WPEP+VRVQSL+ + L  IP+RY++P S RP+          +  IP +D G L+  D +
Sbjct: 7   WPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLT 66

Query: 69  RREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR--------------------- 128
            +  +  ++S+ACRE G FQ++NHG+  ++M + +  WR                     
Sbjct: 67  LQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHANSPKTYE 126

Query: 129 ---SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKM 188
              SR+GV++GA+LDWSDY++LH+ P S ++  K P++P  CR+I E+Y +EM+KL   +
Sbjct: 127 GYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVKLCENL 186

Query: 189 MRVFSENLGLREDYIEKAF-GEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLA 248
           M++ S+NLGL+ED ++ AF G+   GGCLRVN YPKCPQPELTLGIS HSDPGGLTILL 
Sbjct: 187 MKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGGLTILLP 246

Query: 249 DENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
           DE V+        + W+ V+P P+AFI+N+GDQIQ+
Sbjct: 247 DEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQM 282

BLAST of Spg012491 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-68
Identity = 128/274 (46.72%), Postives = 176/274 (64.23%), Query Frame = 0

Query: 9   WPEPVVRVQSLTRTGLHTIPDRYIRPPSSRP----SAADDSIEIPAVDFGELFGGDESRR 68
           WPEP+V VQSL++TG+ T+P+RY++P   RP    + +D  IEIP +D  +++G  E   
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPE--- 64

Query: 69  EASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR----------------------- 128
               R V  AC EWG FQM+NHG+   +M++VR  WR                       
Sbjct: 65  --GLRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGY 124

Query: 129 -SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKMMR 188
            SR+GV + A LDWSDYFFL++ P S RNP+K P+ PP  R++ E+YGEE+ KL  ++  
Sbjct: 125 GSRLGVVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTE 184

Query: 189 VFSENLGLREDYIEKAFGEAG-MGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLADE 248
             SE+LGL+ + + +A G    +G  LR N YPKCPQP+LTLG+S HSDPGG+TILL DE
Sbjct: 185 TLSESLGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDE 244

Query: 249 NVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            V+ GLQV + + WV +K +PNA I+NIGDQ+Q+
Sbjct: 245 KVA-GLQVRRGDGWVTIKSVPNALIVNIGDQLQI 272

BLAST of Spg012491 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.4e-38
Identity = 89/254 (35.04%), Postives = 136/254 (53.54%), Query Frame = 0

Query: 26  TIPDRYIRPPSSRPSAADDS--IEIPAVDFGELFGGDESRREASRRQVSEACREWGIFQM 85
           T+P+ Y+RP S RP  ++ S   + P +D         + R    +Q+ +AC  +G FQ+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDL------SSTDRSFLIQQIHQACARFGFFQV 73

Query: 86  INHGIETEVMKKVRDVWR-------------------------SRMGVQEGAVLDWSDYF 145
           INHG+  +++ ++  V R                         +   V++  V +W DY 
Sbjct: 74  INHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYL 133

Query: 146 FLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKMMRVFSENLGLREDYIEKAFG 205
            LH   P H+  N+ P+ PPS ++I  +Y  E+ ++  K+  + SE+LGL +DY++K  G
Sbjct: 134 RLH-CYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLG 193

Query: 206 EAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLADENVSDGLQVSKANVWVNVKP 253
           E G    + VN YP CP+PELT G+  H+DP  LTILL D  V  GLQ+     W  V P
Sbjct: 194 EQGQH--MAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVC-GLQILIDGQWFAVNP 253

BLAST of Spg012491 vs. ExPASy TrEMBL
Match: A0A2P5F373 (Isopenicillin N synthase OS=Trema orientale OX=63057 GN=TorRG33x02_120600 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 3.0e-84
Identity = 150/277 (54.15%), Postives = 194/277 (70.04%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRP-----SAADDSIEIPAVDFGELFGGDES 67
           TWPEP++RVQSL  +G+  +P RYI+PPS RP     S   ++I IP +   +LF  DE+
Sbjct: 6   TWPEPIIRVQSLAESGIQRLPHRYIKPPSERPQTRVNSQDHETINIPVISLEDLFARDET 65

Query: 68  RREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR--------------------- 127
            +EA+   +S ACREWG FQ++NHG+  E+MK+ RD WR                     
Sbjct: 66  LQEATLAAISSACREWGFFQVVNHGVSPELMKRARDTWREFFGQPLEEKQRYANSPSTYE 125

Query: 128 ---SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKM 187
              SR+GV++GAVLDWSDYFFLHF PPS RNP K P++PPSCR + EEYG E+++L   +
Sbjct: 126 GYGSRLGVEKGAVLDWSDYFFLHFLPPSLRNPEKWPSLPPSCRNVIEEYGNEVVRLCGDL 185

Query: 188 MRVFSENLGLREDYIEKAFG--EAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILL 247
           M++ S NLGL ED+++KAFG      G CLRVN YPKCPQP+LTLG+S HSDPGG+T+LL
Sbjct: 186 MKILSINLGLEEDHLQKAFGGENNNTGACLRVNFYPKCPQPDLTLGLSSHSDPGGMTLLL 245

Query: 248 ADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            DE+V+ GLQV + N W+ VKP+PNAFI+NIGDQIQV
Sbjct: 246 PDEDVA-GLQVRRDNSWITVKPVPNAFIVNIGDQIQV 281

BLAST of Spg012491 vs. ExPASy TrEMBL
Match: A0A2P5BQT0 (Isopenicillin N synthase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_218560 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 8.7e-84
Identity = 151/277 (54.51%), Postives = 193/277 (69.68%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRP-----SAADDSIEIPAVDFGELFGGDES 67
           TWPEP+VRVQSL  +G+  +P RYI+PPS RP     S   ++I IP +   +LF  DE+
Sbjct: 6   TWPEPIVRVQSLAESGIKRLPHRYIKPPSERPQTRVNSQDHETINIPVISLEDLFARDET 65

Query: 68  RREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR--------------------- 127
            +EA+   +S ACREWG FQ++NHG+  E+MK+ RD W                      
Sbjct: 66  LQEATLAAISSACREWGFFQVVNHGVSPELMKQARDTWSEFFGQPLEEKQRYANSPSTYE 125

Query: 128 ---SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKM 187
              SR+GV++GAVLDWSDYFFLHF PPS RNP K P++PPSCR + EEYG E+++L   +
Sbjct: 126 GYGSRLGVEKGAVLDWSDYFFLHFLPPSLRNPEKWPSLPPSCRNVIEEYGNEVVRLCGDL 185

Query: 188 MRVFSENLGLREDYIEKAFG--EAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILL 247
           M++ S NLGL ED+++KAFG      G CLRVN YPKCPQPELTLG+S HSDPGG+T+LL
Sbjct: 186 MKILSINLGLEEDHLQKAFGGENNNTGACLRVNFYPKCPQPELTLGLSSHSDPGGMTLLL 245

Query: 248 ADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            DE+V+ GLQV + N W+ VKP+PNAFI+NIGDQIQV
Sbjct: 246 PDEDVA-GLQVRRDNSWITVKPVPNAFIVNIGDQIQV 281

BLAST of Spg012491 vs. ExPASy TrEMBL
Match: A0A2P6R931 (Putative anthocyanidin synthase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0462961 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 1.1e-81
Identity = 148/275 (53.82%), Postives = 192/275 (69.82%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPS----AADDSIEIPAVDFGELFGGDESR 67
           +WPEP+VRVQSL ++G+ TIP+ YI+PP  RPS      D ++ IP +D   L G D+S 
Sbjct: 7   SWPEPIVRVQSLAQSGIKTIPELYIKPPCKRPSNDLVLDDATVNIPVIDLQALAGDDQSL 66

Query: 68  REASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR---------------------- 127
           RE + + VS ACREWG FQ++NHG+  E+MK+ R+ WR                      
Sbjct: 67  RETTLQLVSSACREWGFFQVVNHGVSHELMKRARETWREFFEQPLEVKQEYSNNPSTYEG 126

Query: 128 --SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKMM 187
             SR+GV++GA+LDWSDY+FLH+ PP  R P K PA+P SCR + EEYGEE  +L  ++M
Sbjct: 127 YGSRLGVEKGAILDWSDYYFLHYMPPELRKPTKWPALPSSCRNLIEEYGEEATRLCGRLM 186

Query: 188 RVFSENLGLREDYIEKAF-GEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLAD 247
           ++ S NLGL EDY+  AF GE  +G CLRVN YPKCPQP+LTLGIS HSDPGG+T+LL D
Sbjct: 187 KMLSLNLGLGEDYLLNAFGGEENVGACLRVNFYPKCPQPDLTLGISAHSDPGGMTLLLPD 246

Query: 248 ENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
           E+V+ GLQV K + WV VKP+PNAFI+N+GDQ+QV
Sbjct: 247 EDVA-GLQVRKGDSWVTVKPVPNAFIVNMGDQMQV 280

BLAST of Spg012491 vs. ExPASy TrEMBL
Match: A0A498K1T5 (Fe2OG dioxygenase domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_014755 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.4e-81
Identity = 152/277 (54.87%), Postives = 187/277 (67.51%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSA------ADDSIEIPAVDFGELFGGDE 67
           +WPEP+VRVQSL  +G+  IPD YI+PPS RPS        D  + IP +D   L   + 
Sbjct: 6   SWPEPIVRVQSLAESGITAIPDLYIKPPSKRPSPPNTNNHRDADVNIPIIDLQNLLSDNR 65

Query: 68  SRREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR-------------------- 127
           S REA+ R +S ACREWG FQ++NHG+  E+MK+ RD WR                    
Sbjct: 66  SLREATMRSISSACREWGFFQVVNHGVSHELMKRARDTWREFFSLPLEVKQEYSNKPSTY 125

Query: 128 ----SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRK 187
               SR+GV++GA+LDWSDY+FLH+ PP  RN NK PA+P SCRK+ EEY EE +KL   
Sbjct: 126 EGYGSRLGVEKGAILDWSDYYFLHYMPPQIRNHNKWPAVPTSCRKLIEEYSEETIKLCGI 185

Query: 188 MMRVFSENLGLREDYIEKAF-GEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILL 247
           +M+V S NLGL ED +  AF GE  +G CLRVN YPKCPQP+LTLG+S HSDPGGLT+LL
Sbjct: 186 LMKVLSLNLGLGEDQLLDAFGGEENIGACLRVNFYPKCPQPDLTLGLSEHSDPGGLTLLL 245

Query: 248 ADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            DENV+ GLQV K   WV VKP+PNAFI+N+GDQ QV
Sbjct: 246 PDENVA-GLQVRKGENWVTVKPVPNAFIVNVGDQTQV 281

BLAST of Spg012491 vs. ExPASy TrEMBL
Match: A0A2P5W4R0 (Fe2OG dioxygenase domain-containing protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA34632 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.4e-81
Identity = 145/271 (53.51%), Postives = 189/271 (69.74%), Query Frame = 0

Query: 8   TWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPS-AADDSIEIPAVDFGELFGGDESRREA 67
           +WPEPVVRVQSL+ +G+  IPDRYI+PPS RP     + ++IP VD   LF  D +  E 
Sbjct: 6   SWPEPVVRVQSLSESGIRAIPDRYIKPPSDRPGFEKQEHVDIPVVDLKNLFSHDHTLHEE 65

Query: 68  SRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR------------------------S 127
           + + +S AC+EWG FQ +NHGI  E+MK+ R+VWR                        S
Sbjct: 66  TLKLISSACKEWGFFQAVNHGISHELMKRTREVWREFFNLPLEVKQEYANAPTTYEGYGS 125

Query: 128 RMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKMMRVF 187
           R+GVQ+GA+LDWSDYFFLH+ P   RNP+K P++P SCR +  EYG E+ KL  ++++  
Sbjct: 126 RLGVQKGAILDWSDYFFLHYLPVYLRNPSKWPSLPSSCRDLISEYGAEVEKLCGRLLKAM 185

Query: 188 SENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLADENVS 247
           S +LGL+EDY++ AFG  G+G CLRVN YPKCPQP+LTLG+S HSDPGGLT+LL D +V+
Sbjct: 186 SISLGLKEDYLQNAFGGEGIGACLRVNFYPKCPQPDLTLGLSSHSDPGGLTLLLPDHDVA 245

Query: 248 DGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            GLQV K   W+ VKP+PNAFI+N+GDQIQV
Sbjct: 246 -GLQVRKNGKWITVKPVPNAFIVNVGDQIQV 275

BLAST of Spg012491 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 283.1 bits (723), Expect = 2.3e-76
Identity = 136/277 (49.10%), Postives = 183/277 (66.06%), Query Frame = 0

Query: 4   GPPHTWPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAADD---SIEIPAVDFGELFGGD 63
           G    WPEP+VRVQSL  + L ++PDRYI+P S RP+  +D   +  IP +D   LF  +
Sbjct: 15  GSMDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEE 74

Query: 64  ESRREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR------------------- 123
               +    ++SEACR WG FQ++NHG++ E+M   R+ WR                   
Sbjct: 75  GLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRT 134

Query: 124 -----SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSR 183
                SR+GV++GA LDWSDY+FLH  P   ++ NK P+ PP+ R++ +EYGEE++KLS 
Sbjct: 135 YEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSG 194

Query: 184 KMMRVFSENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILL 243
           ++MRV S NLGL+ED  ++AFG   +G CLRVN YPKCP+PEL LG+S HSDPGG+TILL
Sbjct: 195 RIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILL 254

Query: 244 ADENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            D+ V  GLQV K + W+ VKP P+AFI+NIGDQIQ+
Sbjct: 255 PDDQVF-GLQVRKDDTWITVKPHPHAFIVNIGDQIQI 290

BLAST of Spg012491 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 2.5e-75
Identity = 131/276 (47.46%), Postives = 183/276 (66.30%), Query Frame = 0

Query: 9   WPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAA-------DDSIEIPAVDFGELFGGDE 68
           WPEP+VRVQSL  + L ++PDRYI+PPS RP             I IP +D   LF G+E
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 69  SRREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR-------------------- 128
                 ++++SEACREWG FQ+INHG++ E+M   R+ W+                    
Sbjct: 109 D----DKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTY 168

Query: 129 ----SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRK 188
               SR+GV++GA+LDW+DY++LHF P + ++ NK P++P + R++ +EYG+E++KL  +
Sbjct: 169 EGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGR 228

Query: 189 MMRVFSENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLA 248
           +M + S NLGLR + +++AFG   +G CLRVN YPKCPQPEL LG+S HSDPGG+TILL 
Sbjct: 229 LMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLP 288

Query: 249 DENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
           D+ V  GLQV   + W+ V PL +AFI+NIGDQIQ+
Sbjct: 289 DDQVV-GLQVRHGDTWITVNPLRHAFIVNIGDQIQI 319

BLAST of Spg012491 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 2.5e-75
Identity = 131/276 (47.46%), Postives = 183/276 (66.30%), Query Frame = 0

Query: 9   WPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAA-------DDSIEIPAVDFGELFGGDE 68
           WPEP+VRVQSL  + L ++PDRYI+PPS RP             I IP +D   LF G+E
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 69  SRREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR-------------------- 128
                 ++++SEACREWG FQ+INHG++ E+M   R+ W+                    
Sbjct: 109 D----DKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTY 168

Query: 129 ----SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRK 188
               SR+GV++GA+LDW+DY++LHF P + ++ NK P++P + R++ +EYG+E++KL  +
Sbjct: 169 EGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGR 228

Query: 189 MMRVFSENLGLREDYIEKAFGEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLA 248
           +M + S NLGLR + +++AFG   +G CLRVN YPKCPQPEL LG+S HSDPGG+TILL 
Sbjct: 229 LMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLP 288

Query: 249 DENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
           D+ V  GLQV   + W+ V PL +AFI+NIGDQIQ+
Sbjct: 289 DDQVV-GLQVRHGDTWITVNPLRHAFIVNIGDQIQI 319

BLAST of Spg012491 vs. TAIR 10
Match: AT3G55970.1 (jasmonate-regulated gene 21 )

HSP 1 Score: 264.6 bits (675), Expect = 8.3e-71
Identity = 126/276 (45.65%), Postives = 180/276 (65.22%), Query Frame = 0

Query: 9   WPEPVVRVQSLTRTGLHTIPDRYIRPPSSRPSAA------DDSIEIPAVDFGELFGGDES 68
           WPEP+VRVQSL+ + L  IP+RY++P S RP+          +  IP +D G L+  D +
Sbjct: 7   WPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLT 66

Query: 69  RREASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR--------------------- 128
            +  +  ++S+ACRE G FQ++NHG+  ++M + +  WR                     
Sbjct: 67  LQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHANSPKTYE 126

Query: 129 ---SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKM 188
              SR+GV++GA+LDWSDY++LH+ P S ++  K P++P  CR+I E+Y +EM+KL   +
Sbjct: 127 GYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVKLCENL 186

Query: 189 MRVFSENLGLREDYIEKAF-GEAGMGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLA 248
           M++ S+NLGL+ED ++ AF G+   GGCLRVN YPKCPQPELTLGIS HSDPGGLTILL 
Sbjct: 187 MKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGGLTILLP 246

Query: 249 DENVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
           DE V+        + W+ V+P P+AFI+N+GDQIQ+
Sbjct: 247 DEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQM 282

BLAST of Spg012491 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 1.6e-69
Identity = 128/274 (46.72%), Postives = 176/274 (64.23%), Query Frame = 0

Query: 9   WPEPVVRVQSLTRTGLHTIPDRYIRPPSSRP----SAADDSIEIPAVDFGELFGGDESRR 68
           WPEP+V VQSL++TG+ T+P+RY++P   RP    + +D  IEIP +D  +++G  E   
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPE--- 64

Query: 69  EASRRQVSEACREWGIFQMINHGIETEVMKKVRDVWR----------------------- 128
               R V  AC EWG FQM+NHG+   +M++VR  WR                       
Sbjct: 65  --GLRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGY 124

Query: 129 -SRMGVQEGAVLDWSDYFFLHFSPPSHRNPNKLPAIPPSCRKITEEYGEEMMKLSRKMMR 188
            SR+GV + A LDWSDYFFL++ P S RNP+K P+ PP  R++ E+YGEE+ KL  ++  
Sbjct: 125 GSRLGVVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTE 184

Query: 189 VFSENLGLREDYIEKAFGEAG-MGGCLRVNLYPKCPQPELTLGISCHSDPGGLTILLADE 248
             SE+LGL+ + + +A G    +G  LR N YPKCPQP+LTLG+S HSDPGG+TILL DE
Sbjct: 185 TLSESLGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDE 244

Query: 249 NVSDGLQVSKANVWVNVKPLPNAFILNIGDQIQV 254
            V+ GLQV + + WV +K +PNA I+NIGDQ+Q+
Sbjct: 245 KVA-GLQVRRGDGWVTIKSVPNALIVNIGDQLQI 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PON92241.16.2e-8454.15Isopenicillin N synthase [Trema orientale][more]
PON51147.11.8e-8354.51Isopenicillin N synthase [Parasponia andersonii][more]
KAF3449533.13.4e-8252.31hypothetical protein FNV43_RR10262 [Rhamnella rubrinervis][more]
XP_004302589.19.9e-8253.51PREDICTED: protein SRG1-like [Fragaria vesca subsp. vesca][more]
XP_024191028.12.2e-8153.82probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Rosa chinensis] >PRQ429... [more]
Match NameE-valueIdentityDescription
Q9FFF63.2e-7549.10Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
Q9SRM33.5e-7447.46Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1[more]
Q9LY481.2e-6945.65Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1[more]
O804492.2e-6846.72Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1[more]
Q9FLV02.4e-3835.04Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A2P5F3733.0e-8454.15Isopenicillin N synthase OS=Trema orientale OX=63057 GN=TorRG33x02_120600 PE=3 S... [more]
A0A2P5BQT08.7e-8454.51Isopenicillin N synthase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_218560 P... [more]
A0A2P6R9311.1e-8153.82Putative anthocyanidin synthase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0462... [more]
A0A498K1T51.4e-8154.87Fe2OG dioxygenase domain-containing protein OS=Malus domestica OX=3750 GN=DVH24_... [more]
A0A2P5W4R01.4e-8153.51Fe2OG dioxygenase domain-containing protein OS=Gossypium barbadense OX=3634 GN=G... [more]
Match NameE-valueIdentityDescription
AT5G05600.12.3e-7649.102-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G11180.12.5e-7547.462-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G11180.22.5e-7547.462-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G55970.18.3e-7145.65jasmonate-regulated gene 21 [more]
AT2G38240.11.6e-6946.722-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 11..260
e-value: 4.8E-67
score: 228.6
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 185..254
e-value: 2.6E-13
score: 50.3
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 48..101
e-value: 1.9E-11
score: 44.8
NoneNo IPR availablePANTHERPTHR47991:SF14LEUCOANTHOCYANIDIN DIOXYGENASE-LIKEcoord: 6..102
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 103..253
NoneNo IPR availablePANTHERPTHR47991:SF14LEUCOANTHOCYANIDIN DIOXYGENASE-LIKEcoord: 103..253
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 6..102
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 12..253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012491.1Spg012491.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity