Spg012413 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012413
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein DETOXIFICATION
Locationscaffold1: 6975049 .. 6977793 (-)
RNA-Seq ExpressionSpg012413
SyntenySpg012413
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACTTTCCTGTTTCTCTCATTCTCGGTCGGCAGCCGGAGAAGCATAAATTTTGTAACCATGGCCGATGACCAACAAACAAACGTTCCGTTATTGGAAGATTCAACTGCAATAATATTACAACCTCGAGATCAAAGTTGTGAAGATATTGTAACGAGGGTGTGGATTGAATCGAAGAAGCTATGGCTTATCGTCGGCCCTGCAATTTTCAGCAGGATAAGCACCTACTCCGTCCTGGTAATCTCCCAAGCCTTCGCCGGCCACTTGGGTGACCTCGACCTCGCCGCCATTTCCATCGCCCTTAACGTCATCATCGGCTTCGATCTCGGACTTATGGTACCCAACCAACAAACGTTCTACCCAATTTCAACTCTGTTTATCTCTGTTCGATTTCTCCTCTGTTTAAACTCTGTTTTTGTGTATTTGAGATTGGAATGGCAAGCGCGTTAGAGACGCTGTGCGGGCAGGCGTACGGGGCGAAGAAGCACTACATGCTGGGAGTGTATCTGCAGCGCTCCTGGATCGTTCTTCTCACATGCTGCCTTTTGCTGTTGCCCGCGTTCGTGTTTGCTTCTCCGATTCTGAAGGTGTTAGGGGAGCCTGATGATCTGGCGGATCTAGTGGGGGTTTTGGCCAGATGGTTGATTCCGTTGCACTTCGGCTTGGCGTTTTACTTCCCATTGCAGAGATTCTTGCAGAGCCAAGTGAAGGCGACGGCGATTATGTGGGTGGCGCTGCTGGGGCTTGGAGTGCACCTGGCGGCCAGTTGGGCGTTTGTCGGTTTCTTCAAAATGGGAGTGGTGGGCATTGCGGTGGCTTCAGATATTTCTTGGTGGGTTCTGCCGATTGGGCTGATGACGTACACTGTCTGCGGCGGCTGCCCCGACACTTGGACTGGCTTCTCCGTCGAAGCCCTCACCGGTGTTTGGGATTTTGTTAAGCTCTCTGCTGCTTCCGGGGTTATGATATGGTCTCTCGCCCTCTAATTAATTAAAACAAAACCACTTTTTTACTTTTCAGTGACACTAAGCCTAAAAACTTCACCAATATTTTCACACGTGGATTTCTTTGAAATTATGCTTGTAATTTTTAAACGTACTTTGACACGTTTTTTTTTAAAATATTGCTAAAAACTAGCTTTTCAAAACTTTGAAGTTTATACGTTTTAAATTTTTTTTTTGTTAGTCTTTCTATCATTAATAAATATTGTTATTGCTTTCATCATTTCTTTGTAATAGAAATTTGAAAACAAAATTCATACCAAACTAAAAATTTAGGATTAGCTTGTGCTTGTCTCTGTTTGTTAAACTGACAATTTACATTTGCAACTGATAAATTTCAGTTTGGAGAATTGGTATTACAGAATATTGATATTGATGACGGGAAACATGGAGAACGCCAAGATTCAAGTGGATGCTTTATCCATCTGGTCTTTCTATGTCCCTCCTCTCTCAAATTCTTCTCAAACATAAAAGATATACTTTTTTAAATTACTTTATAAATACTTAAAAAAATATTTTTAAATAACGGAATATTTGAGTTTATAATAATTTTGTGATGGCAGTATGAGCATCAACGGGTTAGAAATGATGATTCCTCTAGCATTCTTCGCGGGTACAGGGTAATTTAATTATTATTCTAGAAAATATTTTTTCTTCCCAATTATATAAATCTTTACATATAAGACAGACATTAAATTTAAATGAACAGAGTGAGAGTGGCAAATGAGCTCGGGGCAGGAAATGGAAAAGGAGCCAAGTTTGCAGCCATTGTCTCATCAGTGACATCGATGATAATCGGCATTTTCTTTTGTTGTTTGATTGTAATCTTTCACGACAAATTTGGTCTTTTTTACTCCTCCAGCAACGTTGTCCTTGAAGAAGTCAGTAATCTTAGCACCCTCTTGGCCTTCACCATCCTCTTCAATAGTTTCCAACCAGTCCTCTCCGGTATGTCAAATTCTCTATATTTTGTAGTAATCATTAAAATGTATGGTCAAACGATTTCTCTTGTATATTTAGCCAGTAAAATTGTCGACTACATTATTTCAACCATACTAAATATTTGCCTCCAATATATAGGAGTGGCAGTTGGATCTGGTTGGCAATCTTATGTGGCTTATATAAACTTGGGTTGTTATTACCTCATTGGTTTGCCTCTTGGCATCTCGACGCAATGGTTTTTCAACCTTGGTGTTAAGGTAACATATATATATTACACAATTTCTTTTGTTAGAGTCAACCTTTAAGTGATATAATTTGCATTTACCTGTTCAATAATTATATGCTCATTGATCTCTATATAAAACTATATGATTTATTTCATGTATATATATACAAAAGGGAATTTGGATGGGGATGATATTTGGAGGAACAGGTGTTCAGACGTTGATATTGCTCATCATTACTATTCGATGTGATTGGGAAGGAGAGGTATGCTATATCTATTTATATTTACTTAATTTGAACATGCTATTGAATATATATGAGAATTTAATTTCAAGTTTAAATTTATCTCTCTAACATAATTCATCTTCCATTTTGCATTACAAATAATCTTAGGCATTGAATTTCATGAATTAAAAGAATAATATTTCGTCCACAAGTAAAATACAAAGATTAAAAATTTACGGTATTTCATGGATTTAACACTTTTGGTTGTGGATGGATGGGTGATTTGCAGGCTAAGAAAGCAGGTTTGCGCATGCAGAAATGGGACAGATGAAAAGTTTGTGCCGAAGGAATGA

mRNA sequence

ATGGCCGATGACCAACAAACAAACGTTCCGTTATTGGAAGATTCAACTGCAATAATATTACAACCTCGAGATCAAAGTTGTGAAGATATTGTAACGAGGGTGTGGATTGAATCGAAGAAGCTATGGCTTATCGTCGGCCCTGCAATTTTCAGCAGGATAAGCACCTACTCCGTCCTGGTAATCTCCCAAGCCTTCGCCGGCCACTTGGGTGACCTCGACCTCGCCGCCATTTCCATCGCCCTTAACGTCATCATCGGCTTCGATCTCGGACTTATGATTGGAATGGCAAGCGCGTTAGAGACGCTGTGCGGGCAGGCGTACGGGGCGAAGAAGCACTACATGCTGGGAGTGTATCTGCAGCGCTCCTGGATCGTTCTTCTCACATGCTGCCTTTTGCTGTTGCCCGCGTTCGTGTTTGCTTCTCCGATTCTGAAGGTGTTAGGGGAGCCTGATGATCTGGCGGATCTAGTGGGGGTTTTGGCCAGATGGTTGATTCCGTTGCACTTCGGCTTGGCGTTTTACTTCCCATTGCAGAGATTCTTGCAGAGCCAAGTGAAGGCGACGGCGATTATGTGGGTGGCGCTGCTGGGGCTTGGAGTGCACCTGGCGGCCAGTTGGGCGTTTGTCGGTTTCTTCAAAATGGGAGTGGTGGGCATTGCGGTGGCTTCAGATATTTCTTGGTGGGTTCTGCCGATTGGGCTGATGACGTACACTGTCTGCGGCGGCTGCCCCGACACTTGGACTGGCTTCTCCGTCGAAGCCCTCACCGGTGTTTGGGATTTTGTTAAGCTCTCTGCTGCTTCCGGGGTTATGATATGTTTGGAGAATTGGTATTACAGAATATTGATATTGATGACGGGAAACATGGAGAACGCCAAGATTCAAGTGGATGCTTTATCCATCTGTATGAGCATCAACGGGTTAGAAATGATGATTCCTCTAGCATTCTTCGCGGGTACAGGAGTGAGAGTGGCAAATGAGCTCGGGGCAGGAAATGGAAAAGGAGCCAAGTTTGCAGCCATTGTCTCATCAGTGACATCGATGATAATCGGCATTTTCTTTTGTTGTTTGATTGTAATCTTTCACGACAAATTTGGTCTTTTTTACTCCTCCAGCAACGTTGTCCTTGAAGAAGTCAGTAATCTTAGCACCCTCTTGGCCTTCACCATCCTCTTCAATAGTTTCCAACCAGTCCTCTCCGGAGTGGCAGTTGGATCTGGTTGGCAATCTTATGTGGCTTATATAAACTTGGGTTGTTATTACCTCATTGGTTTGCCTCTTGGCATCTCGACGCAATGGTTTTTCAACCTTGGTGTTAAGGGAATTTGGATGGGGATGATATTTGGAGGAACAGGTGTTCAGACGTTGATATTGCTCATCATTACTATTCGATGTGATTGGGAAGGAGAGATTAAAAATTTACGGTATTTCATGGATTTAACACTTTTGGTTGTGGATGGATGGGTGATTTGCAGGCTAAGAAAGCAGGTTTGCGCATGCAGAAATGGGACAGATGAAAAGTTTGTGCCGAAGGAATGA

Coding sequence (CDS)

ATGGCCGATGACCAACAAACAAACGTTCCGTTATTGGAAGATTCAACTGCAATAATATTACAACCTCGAGATCAAAGTTGTGAAGATATTGTAACGAGGGTGTGGATTGAATCGAAGAAGCTATGGCTTATCGTCGGCCCTGCAATTTTCAGCAGGATAAGCACCTACTCCGTCCTGGTAATCTCCCAAGCCTTCGCCGGCCACTTGGGTGACCTCGACCTCGCCGCCATTTCCATCGCCCTTAACGTCATCATCGGCTTCGATCTCGGACTTATGATTGGAATGGCAAGCGCGTTAGAGACGCTGTGCGGGCAGGCGTACGGGGCGAAGAAGCACTACATGCTGGGAGTGTATCTGCAGCGCTCCTGGATCGTTCTTCTCACATGCTGCCTTTTGCTGTTGCCCGCGTTCGTGTTTGCTTCTCCGATTCTGAAGGTGTTAGGGGAGCCTGATGATCTGGCGGATCTAGTGGGGGTTTTGGCCAGATGGTTGATTCCGTTGCACTTCGGCTTGGCGTTTTACTTCCCATTGCAGAGATTCTTGCAGAGCCAAGTGAAGGCGACGGCGATTATGTGGGTGGCGCTGCTGGGGCTTGGAGTGCACCTGGCGGCCAGTTGGGCGTTTGTCGGTTTCTTCAAAATGGGAGTGGTGGGCATTGCGGTGGCTTCAGATATTTCTTGGTGGGTTCTGCCGATTGGGCTGATGACGTACACTGTCTGCGGCGGCTGCCCCGACACTTGGACTGGCTTCTCCGTCGAAGCCCTCACCGGTGTTTGGGATTTTGTTAAGCTCTCTGCTGCTTCCGGGGTTATGATATGTTTGGAGAATTGGTATTACAGAATATTGATATTGATGACGGGAAACATGGAGAACGCCAAGATTCAAGTGGATGCTTTATCCATCTGTATGAGCATCAACGGGTTAGAAATGATGATTCCTCTAGCATTCTTCGCGGGTACAGGAGTGAGAGTGGCAAATGAGCTCGGGGCAGGAAATGGAAAAGGAGCCAAGTTTGCAGCCATTGTCTCATCAGTGACATCGATGATAATCGGCATTTTCTTTTGTTGTTTGATTGTAATCTTTCACGACAAATTTGGTCTTTTTTACTCCTCCAGCAACGTTGTCCTTGAAGAAGTCAGTAATCTTAGCACCCTCTTGGCCTTCACCATCCTCTTCAATAGTTTCCAACCAGTCCTCTCCGGAGTGGCAGTTGGATCTGGTTGGCAATCTTATGTGGCTTATATAAACTTGGGTTGTTATTACCTCATTGGTTTGCCTCTTGGCATCTCGACGCAATGGTTTTTCAACCTTGGTGTTAAGGGAATTTGGATGGGGATGATATTTGGAGGAACAGGTGTTCAGACGTTGATATTGCTCATCATTACTATTCGATGTGATTGGGAAGGAGAGATTAAAAATTTACGGTATTTCATGGATTTAACACTTTTGGTTGTGGATGGATGGGTGATTTGCAGGCTAAGAAAGCAGGTTTGCGCATGCAGAAATGGGACAGATGAAAAGTTTGTGCCGAAGGAATGA

Protein sequence

MADDQQTNVPLLEDSTAIILQPRDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTYSVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGVYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPLQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTYTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVDALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIKNLRYFMDLTLLVVDGWVICRLRKQVCACRNGTDEKFVPKE
Homology
BLAST of Spg012413 vs. NCBI nr
Match: KAG6604053.1 (Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 798.5 bits (2061), Expect = 3.4e-227
Identity = 404/476 (84.87%), Postives = 435/476 (91.39%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQP----RDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTY 60
           M DD QTNVPLLEDST  IL P    +DQ+ EDIVTRVWIESKKLW IVGPA+ SRISTY
Sbjct: 1   MGDDHQTNVPLLEDSTE-ILHPGDPDKDQNSEDIVTRVWIESKKLWQIVGPAVLSRISTY 60

Query: 61  SVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLG 120
           SVLVISQ FAGHLGDLDLAA+SIALNVIIGFD GLM+GMASALETLCGQAYGAKKHYMLG
Sbjct: 61  SVLVISQVFAGHLGDLDLAAVSIALNVIIGFDFGLMMGMASALETLCGQAYGAKKHYMLG 120

Query: 121 VYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFP 180
           VYLQRSW+VL  CC+LLLPA VFAS ILKV+GEPDDLADL GVLARWLIPLHF LAFYFP
Sbjct: 121 VYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYFP 180

Query: 181 LQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMT 240
           LQRFLQSQVKA AIMWVA++GL VH+AASW FVGF KMGVVGIAVA +ISWWVLPIGLM 
Sbjct: 181 LQRFLQSQVKAGAIMWVAMVGLAVHVAASWVFVGFLKMGVVGIAVACNISWWVLPIGLMA 240

Query: 241 YTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQV 300
           YT CGGCP+TWTGFSVEALTG+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+V
Sbjct: 241 YTTCGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEV 300

Query: 301 DALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCC 360
           DALSICMSINGLE+MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSS TS+IIGI FCC
Sbjct: 301 DALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGIVFCC 360

Query: 361 LIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYIN 420
           LIV+  DKFGL +SSS +VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYIN
Sbjct: 361 LIVLLRDKFGLLFSSSTIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYIN 420

Query: 421 LGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIK 473
           LGCYY IGLP+G+ST  F +LGVKGIWMGMIFGGTG+QTLILLIITIRCDWEGE K
Sbjct: 421 LGCYYFIGLPVGMSTLKFSDLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAK 475

BLAST of Spg012413 vs. NCBI nr
Match: XP_023544208.1 (protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 798.5 bits (2061), Expect = 3.4e-227
Identity = 402/476 (84.45%), Postives = 436/476 (91.60%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQP----RDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTY 60
           M DD QTNVPLLEDST  IL P    +DQ+ EDIVTRVWIESKKLW IVGPA+ SRISTY
Sbjct: 1   MGDDHQTNVPLLEDSTE-ILHPGDPDKDQNSEDIVTRVWIESKKLWQIVGPAVLSRISTY 60

Query: 61  SVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLG 120
           SV+VISQ FAGHLGDLDLAA+SIALNVIIGFD GLM+GMASALETLCGQAYGAKKHYMLG
Sbjct: 61  SVIVISQVFAGHLGDLDLAAVSIALNVIIGFDFGLMMGMASALETLCGQAYGAKKHYMLG 120

Query: 121 VYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFP 180
           VYLQRSW+VL  CC+LLLPA VFAS ILKV+GEPDDLADL GVLARWLIPLHF LAFYFP
Sbjct: 121 VYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYFP 180

Query: 181 LQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMT 240
           LQRFLQSQVKA AIMWVA++GL VH+AASW FVGF KMGVVGIAVA +ISWWVLPIGLM 
Sbjct: 181 LQRFLQSQVKARAIMWVAMVGLAVHVAASWVFVGFLKMGVVGIAVACNISWWVLPIGLMA 240

Query: 241 YTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQV 300
           YT+CGGCP+TWTGFSVEALTG+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+V
Sbjct: 241 YTICGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEV 300

Query: 301 DALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCC 360
           DALSICMSINGLE+MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSS TS+IIG+ FCC
Sbjct: 301 DALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGLVFCC 360

Query: 361 LIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYIN 420
           LIV+  DKFGL +SSS +VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYIN
Sbjct: 361 LIVLLRDKFGLLFSSSTIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYIN 420

Query: 421 LGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIK 473
           LGCYY IGLP+G+ST  F +LGVKGIWMGMIFGGTG+QTLILLIITIRCDWEGE K
Sbjct: 421 LGCYYFIGLPVGMSTLKFTHLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAK 475

BLAST of Spg012413 vs. NCBI nr
Match: KAG7034219.1 (Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 797.0 bits (2057), Expect = 9.8e-227
Identity = 402/476 (84.45%), Postives = 435/476 (91.39%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQP----RDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTY 60
           M DD QTNVPLLEDST  IL P    +DQ+ EDIVTRVWIESKKLW IVGPA+ SRISTY
Sbjct: 1   MGDDHQTNVPLLEDSTE-ILHPGDPDKDQNSEDIVTRVWIESKKLWQIVGPAVLSRISTY 60

Query: 61  SVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLG 120
           SV+VISQ FAGHLGDLDLAA+SIALNVIIGFD GLM+GMASALETLCGQAYGAKKHYMLG
Sbjct: 61  SVMVISQVFAGHLGDLDLAAVSIALNVIIGFDFGLMMGMASALETLCGQAYGAKKHYMLG 120

Query: 121 VYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFP 180
           VYLQRSW+VL  CC+LLLPA VFAS ILKV+GEPDDLADL GVLARWLIPLHF LAFYFP
Sbjct: 121 VYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYFP 180

Query: 181 LQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMT 240
           LQRFLQSQVKA AIMWVA++GL VH+AASW FVGF KMGVVGIAVA +ISWWVLPIGLM 
Sbjct: 181 LQRFLQSQVKAGAIMWVAMVGLAVHVAASWVFVGFLKMGVVGIAVACNISWWVLPIGLMA 240

Query: 241 YTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQV 300
           YT CGGCP+TWTGFSVEALTG+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+V
Sbjct: 241 YTTCGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEV 300

Query: 301 DALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCC 360
           DALSICMSINGLE+MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSS TS+IIG+ FCC
Sbjct: 301 DALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGLVFCC 360

Query: 361 LIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYIN 420
           LIV+  DKFGL +SSS +VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYIN
Sbjct: 361 LIVLLRDKFGLLFSSSTIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYIN 420

Query: 421 LGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIK 473
           LGCYY IGLP+G+ST  F +LGVKGIWMGMIFGGTG+QTLILLIITIRCDWEGE K
Sbjct: 421 LGCYYFIGLPVGMSTLKFSDLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAK 475

BLAST of Spg012413 vs. NCBI nr
Match: XP_022950624.1 (protein DETOXIFICATION 27-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 795.8 bits (2054), Expect = 2.2e-226
Identity = 402/476 (84.45%), Postives = 434/476 (91.18%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQP----RDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTY 60
           M DD QTNVPLLEDST  IL P    +DQ+ EDIVTRVWIESKKLW IVGPA+ SRISTY
Sbjct: 1   MGDDHQTNVPLLEDSTE-ILHPGDPDKDQNSEDIVTRVWIESKKLWQIVGPAVLSRISTY 60

Query: 61  SVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLG 120
           SVLVISQ FAGHLGDLDLAA+SIALNVIIGFD GLM+GMASALETLCGQAYGAKKHYMLG
Sbjct: 61  SVLVISQVFAGHLGDLDLAAVSIALNVIIGFDFGLMMGMASALETLCGQAYGAKKHYMLG 120

Query: 121 VYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFP 180
           VYLQRSW+VL  CC+LLLPA VFAS ILKV+GEPDDLADL GVLARWLIPLHF LAFYFP
Sbjct: 121 VYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYFP 180

Query: 181 LQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMT 240
           LQRFLQSQVKA AIMWVA++GL VH+AASW FVG  KMGVVGIAVA +ISWWVLPIGLM 
Sbjct: 181 LQRFLQSQVKARAIMWVAMVGLAVHVAASWVFVGLLKMGVVGIAVACNISWWVLPIGLMA 240

Query: 241 YTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQV 300
           YT CGGCP+TWTGFSVEALTG+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+V
Sbjct: 241 YTTCGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEV 300

Query: 301 DALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCC 360
           DALSICMSINGLE+MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSS TS+IIG+ FCC
Sbjct: 301 DALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGLVFCC 360

Query: 361 LIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYIN 420
           LIV+  DKFGL +SSS +VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYIN
Sbjct: 361 LIVLLRDKFGLLFSSSTIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYIN 420

Query: 421 LGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIK 473
           LGCYY IGLP+G+ST  F +LGVKGIWMGMIFGGTG+QTLILLIITIRCDWEGE K
Sbjct: 421 LGCYYFIGLPVGMSTLKFSDLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAK 475

BLAST of Spg012413 vs. NCBI nr
Match: XP_022978480.1 (protein DETOXIFICATION 27-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 794.7 bits (2051), Expect = 4.9e-226
Identity = 402/482 (83.40%), Postives = 436/482 (90.46%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQP----RDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTY 60
           M DD QTNVPLLEDST  IL P    +DQ+ EDIVTRVW+ESKKLW IVGPA+ SRISTY
Sbjct: 1   MGDDHQTNVPLLEDSTE-ILHPGDPDKDQNSEDIVTRVWVESKKLWQIVGPAVLSRISTY 60

Query: 61  SVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLG 120
           SV+VISQ FAGHLGDLDLAAISIALNVIIGFDLGLM+GMASALETLCGQAYGAKKHYMLG
Sbjct: 61  SVMVISQVFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKKHYMLG 120

Query: 121 VYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFP 180
           VYLQRSW+VL  CC+LLLPA VFAS ILKV+GEPDDLADL GVLARWLIPLHF LAFYFP
Sbjct: 121 VYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYFP 180

Query: 181 LQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMT 240
           LQRFLQSQVKA AIMWVA++GL VH+AASW FVG  KMGVVGIAVA +ISWWVLPIGLM 
Sbjct: 181 LQRFLQSQVKARAIMWVAMVGLAVHVAASWVFVGLLKMGVVGIAVACNISWWVLPIGLMA 240

Query: 241 YTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQV 300
           YT CGGCP+TWTGFSVEALTG+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+V
Sbjct: 241 YTTCGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEV 300

Query: 301 DALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCC 360
           DALSICMSINGLE+MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSS TS+IIG+ FCC
Sbjct: 301 DALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGLVFCC 360

Query: 361 LIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYIN 420
           LIV+  DKFGL +SSS +VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYIN
Sbjct: 361 LIVLLRDKFGLLFSSSAIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYIN 420

Query: 421 LGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIKNLRY 479
           LGCYY IGLP+G+S   F +LGVKGIWMGMIFGGTG+QTLILLIITIRCDWEGE K   +
Sbjct: 421 LGCYYFIGLPVGMSALKFSHLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAKKASF 480

BLAST of Spg012413 vs. ExPASy Swiss-Prot
Match: Q9FKQ1 (Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 3.2e-172
Identity = 301/460 (65.43%), Postives = 365/460 (79.35%), Query Frame = 0

Query: 13  EDSTAIILQPR--DQSCEDIVTRVWIESKKLWLIVGPAIFSRISTYSVLVISQAFAGHLG 72
           E   A++  P   ++  E +  R+ +E+KKLW IVGPAIFSR++TYS+LVI+QAFAGHLG
Sbjct: 11  ESRVALLKSPHTAEEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAFAGHLG 70

Query: 73  DLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGVYLQRSWIVLLTCC 132
           DL+LAAISI  NV +GF+ GL++GMASALETLCGQA+GAKK++MLGVY+QRSWIVL  CC
Sbjct: 71  DLELAAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIVLFFCC 130

Query: 133 LLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPLQRFLQSQVKATAI 192
           +LLLP ++F +P+LK LG+PDD+A+L GV+A W+IPLHF     FPLQRFLQ Q+K    
Sbjct: 131 VLLLPTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKNRVT 190

Query: 193 MWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTYTVCGGCPDTWTGF 252
            + A + L VH+   W FV   K+GVVG      ISWWV  + L+ Y+ CGGCP TWTG 
Sbjct: 191 AYAAAVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPLTWTGL 250

Query: 253 SVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVDALSICMSINGLEM 312
           S EALTG+W+F+KLSA+SGVM+CLENWYYRILI+MTGN++NA+I VD+LSICM+ING EM
Sbjct: 251 SSEALTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAINGWEM 310

Query: 313 MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDKFGLFYS 372
           MIPLAFFAGTGVRVANELGAGNGKGA+FA IVS   S+IIG+FF  LI++ H++    +S
Sbjct: 311 MIPLAFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQIAWIFS 370

Query: 373 SSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGIS 432
           SS  VL+ V+ LS LLAFT+L NS QPVLSGVAVGSGWQSYVAYINLGCYY IG+PLG  
Sbjct: 371 SSVAVLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGVPLGFL 430

Query: 433 TQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGE 471
             W F LGV GIW GMIFGGT VQT+IL  IT+RCDWE E
Sbjct: 431 MGWGFKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKE 470

BLAST of Spg012413 vs. ExPASy Swiss-Prot
Match: Q1PDX9 (Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 1.1e-167
Identity = 289/470 (61.49%), Postives = 366/470 (77.87%), Query Frame = 0

Query: 3   DDQQTNVPLLEDSTAIILQPRDQSCE--DIVTRVWIESKKLWLIVGPAIFSRISTYSVLV 62
           D +      +E++T  +L+  + + E   +   +WIE+KK+W IVGP+IF+ ++TYS+L+
Sbjct: 2   DKKSGGTKAIEEATVPLLECHNAAEEGGGMKREIWIETKKIWYIVGPSIFTGLATYSILI 61

Query: 63  ISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGVYLQ 122
           I+QAFAGHLGDL+LAAISI  N  +GF+ GL++GMASALETLCGQA+GA+++YMLGVY+Q
Sbjct: 62  ITQAFAGHLGDLELAAISIINNFTLGFNYGLLLGMASALETLCGQAFGAREYYMLGVYMQ 121

Query: 123 RSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPLQRF 182
           R WI+L  CC+LLLP ++FA+PILK +G+ DD+A+L G +A W+IP+HF  AF+FPL RF
Sbjct: 122 RYWIILFLCCILLLPMYLFATPILKFIGQSDDIAELTGTIALWVIPVHFAFAFFFPLNRF 181

Query: 183 LQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTYTVC 242
           LQ Q+K   I   A + L VH+   W FV  +K+G++G   + ++ WW+    L  Y+  
Sbjct: 182 LQCQLKNKVIAISAGVSLAVHILVCWFFVYGYKLGIIGTMASVNVPWWLNIFILFLYSTR 241

Query: 243 GGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVDALS 302
           GGC  TWTGFS EA TG+ +  KLSA+SG+M+CLENWYY+IL+LMTGN+ NAKI VD+LS
Sbjct: 242 GGCTLTWTGFSSEAFTGLLELTKLSASSGIMLCLENWYYKILMLMTGNLVNAKIAVDSLS 301

Query: 303 ICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCLIVI 362
           ICMS+NG EMMIPLAFFAGTGVRVANELGAGNGKGA+FA IVS   S++IG+FF  +IVI
Sbjct: 302 ICMSVNGWEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSITLSLMIGLFFTVIIVI 361

Query: 363 FHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINLGCY 422
           FHD+ G  +SSS  VL  V NLS LLAFT+L NS QPVLSGVAVGSGWQSYVAYINLGCY
Sbjct: 362 FHDQIGSIFSSSEAVLNAVDNLSVLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCY 421

Query: 423 YLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGE 471
           YLIGLP G++  W F  GVKGIW GMIFGGT +QTLIL+IIT RCDW+ E
Sbjct: 422 YLIGLPFGLTMGWIFKFGVKGIWAGMIFGGTAIQTLILIIITTRCDWDNE 471

BLAST of Spg012413 vs. ExPASy Swiss-Prot
Match: Q9FNC1 (Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 4.2e-164
Identity = 291/491 (59.27%), Postives = 368/491 (74.95%), Query Frame = 0

Query: 5   QQTNVPLLEDSTAIILQPRDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTYSVLVISQA 64
           ++  +PLL+D         ++   +I   +W+E+KKLW IVGPAIF+R++T  + VI+QA
Sbjct: 13  EKAKIPLLKDQNV-----AEEENGEIKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQA 72

Query: 65  FAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGVYLQRSWI 124
           FAGHLG+L+LAAISI  NVIIGF+  L IGMA+ALETLCGQA+GAKK+ M GVYLQRSWI
Sbjct: 73  FAGHLGELELAAISIVNNVIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWI 132

Query: 125 VLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPLQRFLQSQ 184
           VL    +LLLP ++FA+PILK +G+PDD+A+L G+++ W IP HF  AF+FP+ RFLQ Q
Sbjct: 133 VLFLFSILLLPMYIFATPILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQ 192

Query: 185 VKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTYTVCGGCP 244
           +K + I   + + L VH+   W FV   ++GV+G    +++SWW+    L TYT CGGCP
Sbjct: 193 LKNSVIAISSGVSLVVHIFVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCP 252

Query: 245 DTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVDALSICMS 304
            TWTGFS+E+ T +W+F KLSA+SG+M+CLENWYYR+LI+MTGN+E+A+I VD++SICMS
Sbjct: 253 LTWTGFSMESFTRLWEFTKLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMS 312

Query: 305 INGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDK 364
           INGLEMM+PLAFFAGT VRVANELGAGNGK A+FA I+S   S+IIGI    LI    D+
Sbjct: 313 INGLEMMVPLAFFAGTSVRVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQ 372

Query: 365 FGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINLGCYYLIG 424
            G  +SSS  VL+ V+NLS LL+F IL NS QPVLSGVAVGSGWQS VA+INLGCYY IG
Sbjct: 373 IGWMFSSSETVLKAVNNLSILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIG 432

Query: 425 LPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIKNLRYFMDLTLLV 484
           LPLGI   W F  GVKGIW GMIFGGT VQTLIL+ IT+RCDWE E +N +       + 
Sbjct: 433 LPLGIVMGWMFKFGVKGIWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAK-------VR 491

Query: 485 VDGWVICRLRK 496
           V+ W +   RK
Sbjct: 493 VNKWSVSDARK 491

BLAST of Spg012413 vs. ExPASy Swiss-Prot
Match: Q8W488 (Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.6e-125
Identity = 219/439 (49.89%), Postives = 309/439 (70.39%), Query Frame = 0

Query: 33  RVWIESKKLWLIVGPAIFSRISTYSVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLM 92
           +VWIESKKLW++  PAIF+R ST+ V +ISQ+F GHLG ++LAA SI   V++ F  G++
Sbjct: 32  KVWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIGHLGPIELAAYSITFTVLLRFSNGIL 91

Query: 93  IGMASALETLCGQAYGAKKHYMLGVYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDD 152
           +GMASALETLCGQAYGAK+++MLG+YLQRSWIVL  C + L P ++F+ PIL  LG+ + 
Sbjct: 92  LGMASALETLCGQAYGAKQNHMLGIYLQRSWIVLTGCTICLTPVYIFSGPILLALGQEER 151

Query: 153 LADLVGVLARWLIPLHFGLAFYFPLQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFF 212
           +  +  ++A W+I ++F     F  Q FLQ+Q K   I +VA + LGVH+  SW  +  F
Sbjct: 152 IVRVARIIALWVIGINFSFVPSFTCQMFLQAQSKNKIIAYVAAVSLGVHVFLSWLLMVHF 211

Query: 213 KMGVVGIAVASDISWWVLPIGLMTYTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMI 272
             G+ G   ++ +++W+  I  + +  CGGC DTW GFS+ A   +W   KLS +SG M+
Sbjct: 212 NFGITGAMTSTLVAFWLPNIAQLLFVTCGGCKDTWRGFSMMAFKDLWPVFKLSMSSGGML 271

Query: 273 CLENWYYRILILMTGNMENAKIQVDALSICMSINGLEMMIPLAFFAGTGVRVANELGAGN 332
           CLE WY  IL+L+TGN++NA++ +DAL+IC++INGLEMMI L F A   VRV+NELG+GN
Sbjct: 272 CLELWYNSILVLLTGNLKNAEVALDALAICLNINGLEMMIALGFLAAASVRVSNELGSGN 331

Query: 333 GKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILF 392
            KGAKFA + +  TS+ +GI    + +    +    +++S  V  EV++LS LLAF+IL 
Sbjct: 332 PKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSYIFTTSEAVAAEVADLSPLLAFSILM 391

Query: 393 NSFQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTG 452
           NS QPVLSGVAVG+GWQ YV Y+NL CYYL+G+P+GI   +   L VKG+W+GM+F G  
Sbjct: 392 NSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPIGIILGYVVGLQVKGVWIGMLF-GIF 451

Query: 453 VQTLILLIITIRCDWEGEI 472
           VQT +L ++T+R DW+ ++
Sbjct: 452 VQTCVLTVMTLRTDWDQQV 469

BLAST of Spg012413 vs. ExPASy Swiss-Prot
Match: F4HPH2 (Protein DETOXIFICATION 20 OS=Arabidopsis thaliana OX=3702 GN=DTX20 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 6.1e-123
Identity = 221/458 (48.25%), Postives = 312/458 (68.12%), Query Frame = 0

Query: 33  RVWIESKKLWLIVGPAIFSRISTYSVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLM 92
           +VWIESKKLW++  PAIF+R ST+ V +++QAF GHLG  +LAA SI   +++ F  G++
Sbjct: 29  KVWIESKKLWVVAAPAIFTRYSTFGVSMVTQAFIGHLGPTELAAYSITFTILLRFSNGIL 88

Query: 93  IGMASALETLCGQAYGAKKHYMLGVYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDD 152
           +GMA AL TLCGQAYGAK++ MLG+YLQRSWIVL    + L+P F+FA PIL  LG+ + 
Sbjct: 89  LGMAGALGTLCGQAYGAKQYQMLGIYLQRSWIVLTGGTICLMPVFIFAGPILLALGQEER 148

Query: 153 LADLVGVLARWLIPLHFGLAFYFPLQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFF 212
           +  +  VLA W+I ++F     F  Q FLQ+Q K   I +V  + LG+H+  SW  V  F
Sbjct: 149 IVRVARVLALWVIGINFSFVPSFTCQMFLQAQSKNKIISYVTAVSLGLHVFFSWLLVAHF 208

Query: 213 KMGVVGIAVASDISWWVLPIGLMTYTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMI 272
             G+ G   +  I++W+  I  + Y  CGGC DTW GFS+ A   +W  +KLS +SG M+
Sbjct: 209 NFGITGAMTSMLIAFWLPIIVQLLYVTCGGCKDTWRGFSMLAFKDLWPVLKLSLSSGGML 268

Query: 273 CLENWYYRILILMTGNMENAKIQVDALSICMSINGLEMMIPLAFFAGTGVRVANELGAGN 332
           CLE WY  +L+L+TGN++NA++ +DAL+IC+SIN LEMMI L F A   VRV+NELG+GN
Sbjct: 269 CLELWYNSVLVLLTGNLKNAEVALDALAICISINALEMMIALGFLAAVSVRVSNELGSGN 328

Query: 333 GKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILF 392
            KGAKFA +++  TS+ IGI    + +    +    +++S  V  EV++LS LLAF+IL 
Sbjct: 329 PKGAKFATLIAVFTSLSIGIVLFFVFLFLRGRISYIFTTSEAVAAEVADLSPLLAFSILL 388

Query: 393 NSFQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTG 452
           NS QPVLSGVA+G+GWQ YVAY+NL CYYL+G+P+G+   +   L VKG+W+GM+F G  
Sbjct: 389 NSVQPVLSGVAIGAGWQGYVAYVNLACYYLVGIPIGVILGYVVGLQVKGVWIGMLF-GIF 448

Query: 453 VQTLILLIITIRCDWEGEIKNLRYFMDLTLLVVDGWVI 491
           VQT +L ++T+R DW+ ++         +L  ++ WV+
Sbjct: 449 VQTCVLTVMTLRTDWDQQVST-------SLRNINRWVV 478

BLAST of Spg012413 vs. ExPASy TrEMBL
Match: A0A6J1GGD2 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453668 PE=3 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 1.1e-226
Identity = 402/476 (84.45%), Postives = 434/476 (91.18%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQP----RDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTY 60
           M DD QTNVPLLEDST  IL P    +DQ+ EDIVTRVWIESKKLW IVGPA+ SRISTY
Sbjct: 1   MGDDHQTNVPLLEDSTE-ILHPGDPDKDQNSEDIVTRVWIESKKLWQIVGPAVLSRISTY 60

Query: 61  SVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLG 120
           SVLVISQ FAGHLGDLDLAA+SIALNVIIGFD GLM+GMASALETLCGQAYGAKKHYMLG
Sbjct: 61  SVLVISQVFAGHLGDLDLAAVSIALNVIIGFDFGLMMGMASALETLCGQAYGAKKHYMLG 120

Query: 121 VYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFP 180
           VYLQRSW+VL  CC+LLLPA VFAS ILKV+GEPDDLADL GVLARWLIPLHF LAFYFP
Sbjct: 121 VYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYFP 180

Query: 181 LQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMT 240
           LQRFLQSQVKA AIMWVA++GL VH+AASW FVG  KMGVVGIAVA +ISWWVLPIGLM 
Sbjct: 181 LQRFLQSQVKARAIMWVAMVGLAVHVAASWVFVGLLKMGVVGIAVACNISWWVLPIGLMA 240

Query: 241 YTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQV 300
           YT CGGCP+TWTGFSVEALTG+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+V
Sbjct: 241 YTTCGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEV 300

Query: 301 DALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCC 360
           DALSICMSINGLE+MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSS TS+IIG+ FCC
Sbjct: 301 DALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGLVFCC 360

Query: 361 LIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYIN 420
           LIV+  DKFGL +SSS +VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYIN
Sbjct: 361 LIVLLRDKFGLLFSSSTIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYIN 420

Query: 421 LGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIK 473
           LGCYY IGLP+G+ST  F +LGVKGIWMGMIFGGTG+QTLILLIITIRCDWEGE K
Sbjct: 421 LGCYYFIGLPVGMSTLKFSDLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAK 475

BLAST of Spg012413 vs. ExPASy TrEMBL
Match: A0A6J1IQ80 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478447 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.4e-226
Identity = 402/482 (83.40%), Postives = 436/482 (90.46%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQP----RDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTY 60
           M DD QTNVPLLEDST  IL P    +DQ+ EDIVTRVW+ESKKLW IVGPA+ SRISTY
Sbjct: 1   MGDDHQTNVPLLEDSTE-ILHPGDPDKDQNSEDIVTRVWVESKKLWQIVGPAVLSRISTY 60

Query: 61  SVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLG 120
           SV+VISQ FAGHLGDLDLAAISIALNVIIGFDLGLM+GMASALETLCGQAYGAKKHYMLG
Sbjct: 61  SVMVISQVFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKKHYMLG 120

Query: 121 VYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFP 180
           VYLQRSW+VL  CC+LLLPA VFAS ILKV+GEPDDLADL GVLARWLIPLHF LAFYFP
Sbjct: 121 VYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYFP 180

Query: 181 LQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMT 240
           LQRFLQSQVKA AIMWVA++GL VH+AASW FVG  KMGVVGIAVA +ISWWVLPIGLM 
Sbjct: 181 LQRFLQSQVKARAIMWVAMVGLAVHVAASWVFVGLLKMGVVGIAVACNISWWVLPIGLMA 240

Query: 241 YTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQV 300
           YT CGGCP+TWTGFSVEALTG+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+V
Sbjct: 241 YTTCGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEV 300

Query: 301 DALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCC 360
           DALSICMSINGLE+MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSS TS+IIG+ FCC
Sbjct: 301 DALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGLVFCC 360

Query: 361 LIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYIN 420
           LIV+  DKFGL +SSS +VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYIN
Sbjct: 361 LIVLLRDKFGLLFSSSAIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYIN 420

Query: 421 LGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIKNLRY 479
           LGCYY IGLP+G+S   F +LGVKGIWMGMIFGGTG+QTLILLIITIRCDWEGE K   +
Sbjct: 421 LGCYYFIGLPVGMSALKFSHLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAKKASF 480

BLAST of Spg012413 vs. ExPASy TrEMBL
Match: A0A0A0KLT9 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_6G499160 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.8e-218
Identity = 393/516 (76.16%), Postives = 440/516 (85.27%), Query Frame = 0

Query: 1   MADDQQ-TNVPLLEDSTAIIL---QPRDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTY 60
           M DD Q  +VPLLE+ST I+    +  DQ+ ED+V RVWIESKKLW IVGPAI SR+ST+
Sbjct: 1   MVDDHQIISVPLLEESTPILQSHDRDDDQNSEDLVRRVWIESKKLWYIVGPAILSRVSTH 60

Query: 61  SVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLG 120
           SV+V SQAFAGHLGDLDLAAISIALNVIIGFDLGLM+GMASALETLCGQAYGAK+HYMLG
Sbjct: 61  SVMVTSQAFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKRHYMLG 120

Query: 121 VYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFP 180
           VYLQRSW+VL  CC+LLLP F+FASPILK +GE D+LA+L GVLARWLIPLHF  AFYFP
Sbjct: 121 VYLQRSWMVLFMCCVLLLPVFIFASPILKAIGEGDELAELAGVLARWLIPLHFSFAFYFP 180

Query: 181 LQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMT 240
           LQRFLQSQVKA AIMWVA++GL VH+AASW FVGF KMGVVGIAVA DISWWVLPIGLM 
Sbjct: 181 LQRFLQSQVKARAIMWVAVVGLVVHVAASWVFVGFLKMGVVGIAVACDISWWVLPIGLMG 240

Query: 241 YTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQV 300
           Y+  GGCP TWTGFS+EAL+G+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+V
Sbjct: 241 YSAGGGCPYTWTGFSLEALSGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEV 300

Query: 301 DALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCC 360
           DALSICM INGLE MIPLAFFAGTGVRVANELG GNGKGAKFAAIV+S TS++IG+FFCC
Sbjct: 301 DALSICMGINGLEFMIPLAFFAGTGVRVANELGGGNGKGAKFAAIVASTTSLVIGLFFCC 360

Query: 361 LIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYIN 420
           LIVIFHDKFGL +SSS++VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYIN
Sbjct: 361 LIVIFHDKFGLLFSSSDIVLQEVNRLSILLAFTILFNSIQPVLSGVAVGSGWQSYVAYIN 420

Query: 421 LGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIKNLRY 480
           LGCYY IGLPLGI T  F +LGVKGIW+GMIFGGTG+QT+ILLIITIRCDWE E K    
Sbjct: 421 LGCYYFIGLPLGIFTLRFTHLGVKGIWLGMIFGGTGIQTMILLIITIRCDWEEEAKK--- 480

Query: 481 FMDLTLLVVDGWVICRLRKQVCACRNGTDEKFVPKE 513
                 L +  W               TD+KF+PK+
Sbjct: 481 ----ATLRIQKW---------------TDQKFLPKQ 494

BLAST of Spg012413 vs. ExPASy TrEMBL
Match: A0A1S3B3F7 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103485342 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 5.3e-218
Identity = 386/475 (81.26%), Postives = 427/475 (89.89%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQ---PRDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTYS 60
           MA DQQ +VPLLE+STAI+       DQ+ ED+V RVWIESKKLW IVGPAI SR+ST+S
Sbjct: 1   MAHDQQISVPLLEESTAILQSHDGDDDQNSEDLVRRVWIESKKLWYIVGPAILSRVSTHS 60

Query: 61  VLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGV 120
           V+V SQAFAGHLGDLDLAAISIALNVIIGFDLGLM+GMASALETLCGQAYGAKKHYMLGV
Sbjct: 61  VMVTSQAFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKKHYMLGV 120

Query: 121 YLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPL 180
           YLQRSW+VL  CC+LLLP F+FASPILK +GE D+LA+L G LARWLIPLHF  AFYFPL
Sbjct: 121 YLQRSWMVLFMCCVLLLPVFIFASPILKAIGEGDELAELAGGLARWLIPLHFSFAFYFPL 180

Query: 181 QRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTY 240
           QRFLQSQVKA AIMWVA++GL VH+AASW FVG  KMGVVGIAVA DISWWVLPIGLM Y
Sbjct: 181 QRFLQSQVKARAIMWVAVVGLVVHVAASWVFVGLLKMGVVGIAVACDISWWVLPIGLMGY 240

Query: 241 TVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVD 300
           +  GGCP+TWTGFS+EAL+G+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+VD
Sbjct: 241 SAGGGCPNTWTGFSLEALSGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEVD 300

Query: 301 ALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCL 360
           ALSICM INGLE MIPLAFFAGTGVRVANELG GNGKGAKFAAIVSS TS++IG+ FCCL
Sbjct: 301 ALSICMGINGLEFMIPLAFFAGTGVRVANELGGGNGKGAKFAAIVSSTTSLVIGLLFCCL 360

Query: 361 IVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINL 420
           IVIFHDKFGL +SS+++VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYINL
Sbjct: 361 IVIFHDKFGLLFSSTDIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYINL 420

Query: 421 GCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIK 473
           GCYY IGLPLGI T  F +LGVKGIW+GMIFGGTG+QTLILLIITIRCDWE E K
Sbjct: 421 GCYYFIGLPLGIFTLRFTDLGVKGIWLGMIFGGTGIQTLILLIITIRCDWEEEAK 475

BLAST of Spg012413 vs. ExPASy TrEMBL
Match: A0A5D3CLF0 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003110 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 6.7e-205
Identity = 370/475 (77.89%), Postives = 413/475 (86.95%), Query Frame = 0

Query: 1   MADDQQTNVPLLEDSTAIILQ---PRDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTYS 60
           MA DQQ +VPLLE+STAI+       DQ+ ED+V RVWIESKKLW IVGPAI SR+ST+S
Sbjct: 1   MAHDQQISVPLLEESTAILQSHDGDDDQNSEDLVRRVWIESKKLWYIVGPAILSRVSTHS 60

Query: 61  VLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGV 120
           V+V SQAFAGHLGDLDLAAISIALNVIIGFDLGLM+GMASALETLCGQAYGAKKHYMLGV
Sbjct: 61  VMVTSQAFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKKHYMLGV 120

Query: 121 YLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPL 180
           YLQRSW+VL  CC+LLLP F+FASPILK +GE D+LA+L G LARWLIPLHF  AFYFPL
Sbjct: 121 YLQRSWMVLFMCCVLLLPVFIFASPILKAIGEGDELAELAGGLARWLIPLHFSFAFYFPL 180

Query: 181 QRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTY 240
           QRFLQSQVKA AIMWVA++GL VH+AASW FVG  KMGVVGIAVA DISWWVLPIGLM Y
Sbjct: 181 QRFLQSQVKARAIMWVAVVGLVVHVAASWVFVGLLKMGVVGIAVACDISWWVLPIGLMGY 240

Query: 241 TVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVD 300
           +  GGCP+TWTGFS+EAL+G+WDF+KLSAASGVM+CLENWYY+ILI+MTGNM+NAKI+VD
Sbjct: 241 SAGGGCPNTWTGFSLEALSGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIEVD 300

Query: 301 ALSICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCL 360
           ALSI          I L +F    +RVANELG GNGKGAKFAAIVSS TS++IG+ FCCL
Sbjct: 301 ALSIWSFSISFFTSIILVYF----LRVANELGGGNGKGAKFAAIVSSTTSLVIGLLFCCL 360

Query: 361 IVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINL 420
           IVIFHDKFGL +SS+++VL+EV+ LS LLAFTILFNS QPVLSGVAVGSGWQSYVAYINL
Sbjct: 361 IVIFHDKFGLLFSSTDIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYINL 420

Query: 421 GCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIK 473
           GCYY IGLPLGI T  F +LGVKGIW+GMIFGGTG+QTLILLIITIRCDWE E K
Sbjct: 421 GCYYFIGLPLGIFTLRFTDLGVKGIWLGMIFGGTGIQTLILLIITIRCDWEEEAK 471

BLAST of Spg012413 vs. TAIR 10
Match: AT5G65380.1 (MATE efflux family protein )

HSP 1 Score: 606.3 bits (1562), Expect = 2.3e-173
Identity = 301/460 (65.43%), Postives = 365/460 (79.35%), Query Frame = 0

Query: 13  EDSTAIILQPR--DQSCEDIVTRVWIESKKLWLIVGPAIFSRISTYSVLVISQAFAGHLG 72
           E   A++  P   ++  E +  R+ +E+KKLW IVGPAIFSR++TYS+LVI+QAFAGHLG
Sbjct: 11  ESRVALLKSPHTAEEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAFAGHLG 70

Query: 73  DLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGVYLQRSWIVLLTCC 132
           DL+LAAISI  NV +GF+ GL++GMASALETLCGQA+GAKK++MLGVY+QRSWIVL  CC
Sbjct: 71  DLELAAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIVLFFCC 130

Query: 133 LLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPLQRFLQSQVKATAI 192
           +LLLP ++F +P+LK LG+PDD+A+L GV+A W+IPLHF     FPLQRFLQ Q+K    
Sbjct: 131 VLLLPTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKNRVT 190

Query: 193 MWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTYTVCGGCPDTWTGF 252
            + A + L VH+   W FV   K+GVVG      ISWWV  + L+ Y+ CGGCP TWTG 
Sbjct: 191 AYAAAVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPLTWTGL 250

Query: 253 SVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVDALSICMSINGLEM 312
           S EALTG+W+F+KLSA+SGVM+CLENWYYRILI+MTGN++NA+I VD+LSICM+ING EM
Sbjct: 251 SSEALTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAINGWEM 310

Query: 313 MIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDKFGLFYS 372
           MIPLAFFAGTGVRVANELGAGNGKGA+FA IVS   S+IIG+FF  LI++ H++    +S
Sbjct: 311 MIPLAFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQIAWIFS 370

Query: 373 SSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGIS 432
           SS  VL+ V+ LS LLAFT+L NS QPVLSGVAVGSGWQSYVAYINLGCYY IG+PLG  
Sbjct: 371 SSVAVLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGVPLGFL 430

Query: 433 TQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGE 471
             W F LGV GIW GMIFGGT VQT+IL  IT+RCDWE E
Sbjct: 431 MGWGFKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKE 470

BLAST of Spg012413 vs. TAIR 10
Match: AT5G10420.1 (MATE efflux family protein )

HSP 1 Score: 591.3 bits (1523), Expect = 7.6e-169
Identity = 289/470 (61.49%), Postives = 366/470 (77.87%), Query Frame = 0

Query: 3   DDQQTNVPLLEDSTAIILQPRDQSCE--DIVTRVWIESKKLWLIVGPAIFSRISTYSVLV 62
           D +      +E++T  +L+  + + E   +   +WIE+KK+W IVGP+IF+ ++TYS+L+
Sbjct: 2   DKKSGGTKAIEEATVPLLECHNAAEEGGGMKREIWIETKKIWYIVGPSIFTGLATYSILI 61

Query: 63  ISQAFAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGVYLQ 122
           I+QAFAGHLGDL+LAAISI  N  +GF+ GL++GMASALETLCGQA+GA+++YMLGVY+Q
Sbjct: 62  ITQAFAGHLGDLELAAISIINNFTLGFNYGLLLGMASALETLCGQAFGAREYYMLGVYMQ 121

Query: 123 RSWIVLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPLQRF 182
           R WI+L  CC+LLLP ++FA+PILK +G+ DD+A+L G +A W+IP+HF  AF+FPL RF
Sbjct: 122 RYWIILFLCCILLLPMYLFATPILKFIGQSDDIAELTGTIALWVIPVHFAFAFFFPLNRF 181

Query: 183 LQSQVKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTYTVC 242
           LQ Q+K   I   A + L VH+   W FV  +K+G++G   + ++ WW+    L  Y+  
Sbjct: 182 LQCQLKNKVIAISAGVSLAVHILVCWFFVYGYKLGIIGTMASVNVPWWLNIFILFLYSTR 241

Query: 243 GGCPDTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVDALS 302
           GGC  TWTGFS EA TG+ +  KLSA+SG+M+CLENWYY+IL+LMTGN+ NAKI VD+LS
Sbjct: 242 GGCTLTWTGFSSEAFTGLLELTKLSASSGIMLCLENWYYKILMLMTGNLVNAKIAVDSLS 301

Query: 303 ICMSINGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCLIVI 362
           ICMS+NG EMMIPLAFFAGTGVRVANELGAGNGKGA+FA IVS   S++IG+FF  +IVI
Sbjct: 302 ICMSVNGWEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSITLSLMIGLFFTVIIVI 361

Query: 363 FHDKFGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINLGCY 422
           FHD+ G  +SSS  VL  V NLS LLAFT+L NS QPVLSGVAVGSGWQSYVAYINLGCY
Sbjct: 362 FHDQIGSIFSSSEAVLNAVDNLSVLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCY 421

Query: 423 YLIGLPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGE 471
           YLIGLP G++  W F  GVKGIW GMIFGGT +QTLIL+IIT RCDW+ E
Sbjct: 422 YLIGLPFGLTMGWIFKFGVKGIWAGMIFGGTAIQTLILIIITTRCDWDNE 471

BLAST of Spg012413 vs. TAIR 10
Match: AT5G44050.1 (MATE efflux family protein )

HSP 1 Score: 579.3 bits (1492), Expect = 3.0e-165
Identity = 291/491 (59.27%), Postives = 368/491 (74.95%), Query Frame = 0

Query: 5   QQTNVPLLEDSTAIILQPRDQSCEDIVTRVWIESKKLWLIVGPAIFSRISTYSVLVISQA 64
           ++  +PLL+D         ++   +I   +W+E+KKLW IVGPAIF+R++T  + VI+QA
Sbjct: 13  EKAKIPLLKDQNV-----AEEENGEIKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQA 72

Query: 65  FAGHLGDLDLAAISIALNVIIGFDLGLMIGMASALETLCGQAYGAKKHYMLGVYLQRSWI 124
           FAGHLG+L+LAAISI  NVIIGF+  L IGMA+ALETLCGQA+GAKK+ M GVYLQRSWI
Sbjct: 73  FAGHLGELELAAISIVNNVIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWI 132

Query: 125 VLLTCCLLLLPAFVFASPILKVLGEPDDLADLVGVLARWLIPLHFGLAFYFPLQRFLQSQ 184
           VL    +LLLP ++FA+PILK +G+PDD+A+L G+++ W IP HF  AF+FP+ RFLQ Q
Sbjct: 133 VLFLFSILLLPMYIFATPILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQ 192

Query: 185 VKATAIMWVALLGLGVHLAASWAFVGFFKMGVVGIAVASDISWWVLPIGLMTYTVCGGCP 244
           +K + I   + + L VH+   W FV   ++GV+G    +++SWW+    L TYT CGGCP
Sbjct: 193 LKNSVIAISSGVSLVVHIFVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCP 252

Query: 245 DTWTGFSVEALTGVWDFVKLSAASGVMICLENWYYRILILMTGNMENAKIQVDALSICMS 304
            TWTGFS+E+ T +W+F KLSA+SG+M+CLENWYYR+LI+MTGN+E+A+I VD++SICMS
Sbjct: 253 LTWTGFSMESFTRLWEFTKLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMS 312

Query: 305 INGLEMMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDK 364
           INGLEMM+PLAFFAGT VRVANELGAGNGK A+FA I+S   S+IIGI    LI    D+
Sbjct: 313 INGLEMMVPLAFFAGTSVRVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQ 372

Query: 365 FGLFYSSSNVVLEEVSNLSTLLAFTILFNSFQPVLSGVAVGSGWQSYVAYINLGCYYLIG 424
            G  +SSS  VL+ V+NLS LL+F IL NS QPVLSGVAVGSGWQS VA+INLGCYY IG
Sbjct: 373 IGWMFSSSETVLKAVNNLSILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIG 432

Query: 425 LPLGISTQWFFNLGVKGIWMGMIFGGTGVQTLILLIITIRCDWEGEIKNLRYFMDLTLLV 484
           LPLGI   W F  GVKGIW GMIFGGT VQTLIL+ IT+RCDWE E +N +       + 
Sbjct: 433 LPLGIVMGWMFKFGVKGIWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAK-------VR 491

Query: 485 VDGWVICRLRK 496
           V+ W +   RK
Sbjct: 493 VNKWSVSDARK 491

BLAST of Spg012413 vs. TAIR 10
Match: AT1G33110.1 (MATE efflux family protein )

HSP 1 Score: 449.1 bits (1154), Expect = 4.7e-126
Identity = 219/439 (49.89%), Postives = 309/439 (70.39%), Query Frame = 0

Query: 33  RVWIESKKLWLIVGPAIFSRISTYSVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLM 92
           +VWIESKKLW++  PAIF+R ST+ V +ISQ+F GHLG ++LAA SI   V++ F  G++
Sbjct: 32  KVWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIGHLGPIELAAYSITFTVLLRFSNGIL 91

Query: 93  IGMASALETLCGQAYGAKKHYMLGVYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDD 152
           +GMASALETLCGQAYGAK+++MLG+YLQRSWIVL  C + L P ++F+ PIL  LG+ + 
Sbjct: 92  LGMASALETLCGQAYGAKQNHMLGIYLQRSWIVLTGCTICLTPVYIFSGPILLALGQEER 151

Query: 153 LADLVGVLARWLIPLHFGLAFYFPLQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFF 212
           +  +  ++A W+I ++F     F  Q FLQ+Q K   I +VA + LGVH+  SW  +  F
Sbjct: 152 IVRVARIIALWVIGINFSFVPSFTCQMFLQAQSKNKIIAYVAAVSLGVHVFLSWLLMVHF 211

Query: 213 KMGVVGIAVASDISWWVLPIGLMTYTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMI 272
             G+ G   ++ +++W+  I  + +  CGGC DTW GFS+ A   +W   KLS +SG M+
Sbjct: 212 NFGITGAMTSTLVAFWLPNIAQLLFVTCGGCKDTWRGFSMMAFKDLWPVFKLSMSSGGML 271

Query: 273 CLENWYYRILILMTGNMENAKIQVDALSICMSINGLEMMIPLAFFAGTGVRVANELGAGN 332
           CLE WY  IL+L+TGN++NA++ +DAL+IC++INGLEMMI L F A   VRV+NELG+GN
Sbjct: 272 CLELWYNSILVLLTGNLKNAEVALDALAICLNINGLEMMIALGFLAAASVRVSNELGSGN 331

Query: 333 GKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILF 392
            KGAKFA + +  TS+ +GI    + +    +    +++S  V  EV++LS LLAF+IL 
Sbjct: 332 PKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSYIFTTSEAVAAEVADLSPLLAFSILM 391

Query: 393 NSFQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTG 452
           NS QPVLSGVAVG+GWQ YV Y+NL CYYL+G+P+GI   +   L VKG+W+GM+F G  
Sbjct: 392 NSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPIGIILGYVVGLQVKGVWIGMLF-GIF 451

Query: 453 VQTLILLIITIRCDWEGEI 472
           VQT +L ++T+R DW+ ++
Sbjct: 452 VQTCVLTVMTLRTDWDQQV 469

BLAST of Spg012413 vs. TAIR 10
Match: AT1G33100.1 (MATE efflux family protein )

HSP 1 Score: 442.6 bits (1137), Expect = 4.4e-124
Identity = 221/458 (48.25%), Postives = 312/458 (68.12%), Query Frame = 0

Query: 33  RVWIESKKLWLIVGPAIFSRISTYSVLVISQAFAGHLGDLDLAAISIALNVIIGFDLGLM 92
           +VWIESKKLW++  PAIF+R ST+ V +++QAF GHLG  +LAA SI   +++ F  G++
Sbjct: 29  KVWIESKKLWVVAAPAIFTRYSTFGVSMVTQAFIGHLGPTELAAYSITFTILLRFSNGIL 88

Query: 93  IGMASALETLCGQAYGAKKHYMLGVYLQRSWIVLLTCCLLLLPAFVFASPILKVLGEPDD 152
           +GMA AL TLCGQAYGAK++ MLG+YLQRSWIVL    + L+P F+FA PIL  LG+ + 
Sbjct: 89  LGMAGALGTLCGQAYGAKQYQMLGIYLQRSWIVLTGGTICLMPVFIFAGPILLALGQEER 148

Query: 153 LADLVGVLARWLIPLHFGLAFYFPLQRFLQSQVKATAIMWVALLGLGVHLAASWAFVGFF 212
           +  +  VLA W+I ++F     F  Q FLQ+Q K   I +V  + LG+H+  SW  V  F
Sbjct: 149 IVRVARVLALWVIGINFSFVPSFTCQMFLQAQSKNKIISYVTAVSLGLHVFFSWLLVAHF 208

Query: 213 KMGVVGIAVASDISWWVLPIGLMTYTVCGGCPDTWTGFSVEALTGVWDFVKLSAASGVMI 272
             G+ G   +  I++W+  I  + Y  CGGC DTW GFS+ A   +W  +KLS +SG M+
Sbjct: 209 NFGITGAMTSMLIAFWLPIIVQLLYVTCGGCKDTWRGFSMLAFKDLWPVLKLSLSSGGML 268

Query: 273 CLENWYYRILILMTGNMENAKIQVDALSICMSINGLEMMIPLAFFAGTGVRVANELGAGN 332
           CLE WY  +L+L+TGN++NA++ +DAL+IC+SIN LEMMI L F A   VRV+NELG+GN
Sbjct: 269 CLELWYNSVLVLLTGNLKNAEVALDALAICISINALEMMIALGFLAAVSVRVSNELGSGN 328

Query: 333 GKGAKFAAIVSSVTSMIIGIFFCCLIVIFHDKFGLFYSSSNVVLEEVSNLSTLLAFTILF 392
            KGAKFA +++  TS+ IGI    + +    +    +++S  V  EV++LS LLAF+IL 
Sbjct: 329 PKGAKFATLIAVFTSLSIGIVLFFVFLFLRGRISYIFTTSEAVAAEVADLSPLLAFSILL 388

Query: 393 NSFQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGISTQWFFNLGVKGIWMGMIFGGTG 452
           NS QPVLSGVA+G+GWQ YVAY+NL CYYL+G+P+G+   +   L VKG+W+GM+F G  
Sbjct: 389 NSVQPVLSGVAIGAGWQGYVAYVNLACYYLVGIPIGVILGYVVGLQVKGVWIGMLF-GIF 448

Query: 453 VQTLILLIITIRCDWEGEIKNLRYFMDLTLLVVDGWVI 491
           VQT +L ++T+R DW+ ++         +L  ++ WV+
Sbjct: 449 VQTCVLTVMTLRTDWDQQVST-------SLRNINRWVV 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6604053.13.4e-22784.87Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023544208.13.4e-22784.45protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo][more]
KAG7034219.19.8e-22784.45Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022950624.12.2e-22684.45protein DETOXIFICATION 27-like isoform X1 [Cucurbita moschata][more]
XP_022978480.14.9e-22683.40protein DETOXIFICATION 27-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FKQ13.2e-17265.43Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1[more]
Q1PDX91.1e-16761.49Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1[more]
Q9FNC14.2e-16459.27Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1[more]
Q8W4886.6e-12549.89Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1[more]
F4HPH26.1e-12348.25Protein DETOXIFICATION 20 OS=Arabidopsis thaliana OX=3702 GN=DTX20 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GGD21.1e-22684.45Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453668 PE=3 SV=1[more]
A0A6J1IQ802.4e-22683.40Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478447 PE=3 SV=1[more]
A0A0A0KLT91.8e-21876.16Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_6G499160 PE=3 SV=1[more]
A0A1S3B3F75.3e-21881.26Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103485342 PE=3 SV=1[more]
A0A5D3CLF06.7e-20577.89Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
Match NameE-valueIdentityDescription
AT5G65380.12.3e-17365.43MATE efflux family protein [more]
AT5G10420.17.6e-16961.49MATE efflux family protein [more]
AT5G44050.13.0e-16559.27MATE efflux family protein [more]
AT1G33110.14.7e-12649.89MATE efflux family protein [more]
AT1G33100.14.4e-12448.25MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 269..428
e-value: 1.1E-25
score: 90.1
coord: 47..205
e-value: 7.3E-30
score: 103.7
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 47..443
e-value: 6.8E-64
score: 214.1
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 20..476
NoneNo IPR availablePANTHERPTHR11206:SF194PROTEIN DETOXIFICATIONcoord: 20..476
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 37..473
e-value: 2.30659E-154
score: 445.479

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012413.1Spg012413.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity