Spg012119 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg012119 vs. NCBI nr
Match: XP_022962911.1 (putative U-box domain-containing protein 50 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 698/805 (86.71%), Postives = 746/805 (92.67%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           M GKA+KVYVAIGNDLHDGFKTLGWTL KWK+ PISIVILH+AYT          GKLPA
Sbjct: 1   MTGKADKVYVAIGNDLHDGFKTLGWTLGKWKTQPISIVILHIAYTNSQEYVYTPFGKLPA 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKV+VLR+YEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEP+++ LVDLISELGI
Sbjct: 61  SSVSEEKVKVLRKYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPIHRFLVDLISELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKA+ CELFVIWGGKQVFLRDERIMEDDKGV
Sbjct: 121 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKANSCELFVIWGGKQVFLRDERIMEDDKGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KISSK S K WLG++F+EDP Y +ER LSLSS+SPSSSNS SSRNYWD+N+HELENY
Sbjct: 181 RISKISSKHSFKDWLGKMFMEDPIYSTERMLSLSSSSPSSSNSLSSRNYWDNNIHELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           YEELLSLNLQEE+DCEQDQ+  LE+S STQFN+LDHPDS+MN EE IEHLRTKIE+ARKT
Sbjct: 241 YEELLSLNLQEEKDCEQDQEDVLETSSSTQFNILDHPDSDMNTEESIEHLRTKIEEARKT 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVT+REEL+KELD  KE ILEI
Sbjct: 301 IQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTIREELQKELDPAKECILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLSSLLELQAELS KL IST GKSRIEAQLEKTAKTREGMVREIE+LRRQR
Sbjct: 361 VRDIEESKNRLSSLLELQAELSRKLQISTAGKSRIEAQLEKTAKTREGMVREIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           DIL+RRIEFCKEKDAIGMAERSTEV+CSTRVYT EEIRLAT+ FSEQMRLSS++YRGRIN
Sbjct: 421 DILNRRIEFCKEKDAIGMAERSTEVTCSTRVYTGEEIRLATEGFSEQMRLSSQVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H S+AIQMIDSGN LSEEDFQSKVELLS+IRHPHLIA+MGFCSELKCIVFDYMH GSLSD
Sbjct: 481 HNSIAIQMIDSGNCLSEEDFQSKVELLSHIRHPHLIAMMGFCSELKCIVFDYMHNGSLSD 540

Query: 591 RLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLV 650
           RLLP+N+RSRKI R LMWHDRIRIASEVCSGLSFLHQAQPQPISHG+LTLSKILLDQNLV
Sbjct: 541 RLLPTNRRSRKIRRPLMWHDRIRIASEVCSGLSFLHQAQPQPISHGNLTLSKILLDQNLV 600

Query: 651 AKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDDKA 710
           AK+TG GL ELDEAGGIELDIRAFGALLLHLVTGRNW GLIEK LTMGKVG+VQILDD+A
Sbjct: 601 AKVTGFGLAELDEAGGIELDIRAFGALLLHLVTGRNWTGLIEKALTMGKVGIVQILDDEA 660

Query: 711 GKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGA 770
           G+WPLDLVD LVDLALRCVTPNG NSE+NL TAMEEIDEIK +ADDLV       G EGA
Sbjct: 661 GQWPLDLVDGLVDLALRCVTPNGLNSELNLATAMEEIDEIKAKADDLV-------GNEGA 720

Query: 771 DAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 830
           +A NEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH
Sbjct: 721 NATNEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 780

Query: 831 PYLTPNHTLRSLIQDWQNETSNVYT 847
           PYLTPNHTLRSLIQDWQNETSN+YT
Sbjct: 781 PYLTPNHTLRSLIQDWQNETSNLYT 798

BLAST of Spg012119 vs. NCBI nr
Match: XP_023518889.1 (putative U-box domain-containing protein 50 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1351.7 bits (3497), Expect = 0.0e+00
Identity = 696/805 (86.46%), Postives = 746/805 (92.67%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           M GKA+KVYVAIGNDLHDGFKTLGWTL KWKS PISIVILH+AYT          GKLPA
Sbjct: 1   MTGKADKVYVAIGNDLHDGFKTLGWTLGKWKSQPISIVILHIAYTNSQEYVYTPFGKLPA 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSE+KV+VLR+YEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEP+++ LVDLISELGI
Sbjct: 61  SSVSEDKVKVLRKYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPIHRFLVDLISELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKA+ CELFVIWGGKQVFLRDERIMEDDKGV
Sbjct: 121 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKANSCELFVIWGGKQVFLRDERIMEDDKGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KISSK S K WLG++F+EDP Y +ER LSLSS+SPSSSNS SSRNYWD+N+HELENY
Sbjct: 181 RISKISSKHSFKDWLGKMFMEDPIYSTERMLSLSSSSPSSSNSLSSRNYWDNNIHELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           YEELLSLNLQEE+DCEQDQ+  LE+S STQFN+LDHPDS+MN EE IEHLRTKIE+ARKT
Sbjct: 241 YEELLSLNLQEEKDCEQDQEDVLETSSSTQFNILDHPDSDMNTEESIEHLRTKIEEARKT 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVT+REEL+KELD  KE ILEI
Sbjct: 301 IQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTIREELQKELDPAKECILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLSSLLELQAELS KL IST GKSRIEAQLEKTAKT+EGMVREIE+LRRQR
Sbjct: 361 VRDIEESKNRLSSLLELQAELSRKLQISTAGKSRIEAQLEKTAKTKEGMVREIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           DIL+RRIEFCKEKDAIGMAERS+EV+CSTRVYTAEEIRLAT+ FSEQMRLSSR+YRGRIN
Sbjct: 421 DILNRRIEFCKEKDAIGMAERSSEVTCSTRVYTAEEIRLATEGFSEQMRLSSRVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H S+AIQMIDSGN LSEEDFQSKVELLS+IRHPHLIA+MGFCSELKCIVFDYMH GSLSD
Sbjct: 481 HNSIAIQMIDSGNCLSEEDFQSKVELLSHIRHPHLIAMMGFCSELKCIVFDYMHNGSLSD 540

Query: 591 RLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLV 650
           RLLP+NKRSRKI R LMWHDRIRIASEVCSGLSFLHQAQPQPISHG+LTLSKILLDQNLV
Sbjct: 541 RLLPTNKRSRKIRRPLMWHDRIRIASEVCSGLSFLHQAQPQPISHGNLTLSKILLDQNLV 600

Query: 651 AKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDDKA 710
           AK+TG GL ELDEAGGI LDI+AFGALLLHLVTGRNW GLIEK LTMGKVG+VQILDD+A
Sbjct: 601 AKVTGFGLAELDEAGGIALDIQAFGALLLHLVTGRNWTGLIEKALTMGKVGIVQILDDEA 660

Query: 711 GKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGA 770
           G+WPLDLVD LVDLALRCVTPNG NSE+NL TAMEEIDEIK +ADDLV       G EGA
Sbjct: 661 GQWPLDLVDGLVDLALRCVTPNGLNSELNLATAMEEIDEIKAKADDLV-------GNEGA 720

Query: 771 DAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 830
           +A NEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIE+WIIAGHDTSPMTNLRLQH
Sbjct: 721 NATNEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEKWIIAGHDTSPMTNLRLQH 780

Query: 831 PYLTPNHTLRSLIQDWQNETSNVYT 847
           PYLTPNHTLRSLIQDWQNETSN+YT
Sbjct: 781 PYLTPNHTLRSLIQDWQNETSNLYT 798

BLAST of Spg012119 vs. NCBI nr
Match: XP_023002977.1 (putative U-box domain-containing protein 50 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 696/805 (86.46%), Postives = 743/805 (92.30%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           M GKA+KVYVAIGNDLHDGFKTLGWTL KWKS PISIVILH+AYT          GKLPA
Sbjct: 1   MTGKADKVYVAIGNDLHDGFKTLGWTLGKWKSQPISIVILHIAYTNSQEYVYTPFGKLPA 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKV+VLR+YEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEP+++ LVDLISELGI
Sbjct: 61  SSVSEEKVKVLRKYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPIHRFLVDLISELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKA+ CELFVIWGGKQVFLRDERIMEDDKGV
Sbjct: 121 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKANSCELFVIWGGKQVFLRDERIMEDDKGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KIS+K S K WLG++FVEDP Y +ER LSLSS+SPSSSNS SSRNYWD+N+HELENY
Sbjct: 181 RISKISTKHSFKDWLGKMFVEDPIYSTERMLSLSSSSPSSSNSLSSRNYWDNNIHELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           Y+ELLSLNLQEE+DCEQDQ+  LE+S STQFN+LDHPDS+MN EE IEHLRTKIE+ARKT
Sbjct: 241 YKELLSLNLQEEKDCEQDQEDVLETSSSTQFNILDHPDSDMNTEESIEHLRTKIEEARKT 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVT+REEL+KELD  KE ILEI
Sbjct: 301 IQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTIREELQKELDPAKECILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLS LLELQAELS KL IST GKSRIEAQLEKTAKTREGMVREIE+LRRQR
Sbjct: 361 VRDIEESKNRLSPLLELQAELSRKLQISTAGKSRIEAQLEKTAKTREGMVREIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           DIL+RRIEFCKEKDAIGMAERSTEV+CSTRVYTAEEIRLAT+ FSEQMRLSSR+YRGRIN
Sbjct: 421 DILNRRIEFCKEKDAIGMAERSTEVTCSTRVYTAEEIRLATEGFSEQMRLSSRVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H S+AIQMIDSGN LSEEDFQSKVELLS+IRHPHLIA+MGFCSELKCIVFDYMH GSLSD
Sbjct: 481 HNSIAIQMIDSGNCLSEEDFQSKVELLSHIRHPHLIAMMGFCSELKCIVFDYMHSGSLSD 540

Query: 591 RLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLV 650
           RLLP+NKRSRKI R LMWHDRIRIASEVCSGLSFLHQAQPQPISHG+LTLSKILLDQNLV
Sbjct: 541 RLLPTNKRSRKIRRPLMWHDRIRIASEVCSGLSFLHQAQPQPISHGNLTLSKILLDQNLV 600

Query: 651 AKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDDKA 710
           AK+TG GL ELD+AGGIELDIRAFGALLLHLVTGRNW GLIEK LTMGK G+VQILDD+A
Sbjct: 601 AKVTGFGLAELDKAGGIELDIRAFGALLLHLVTGRNWTGLIEKALTMGKAGIVQILDDEA 660

Query: 711 GKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGA 770
           G+WPLDLVD LVDLAL CVTPNG NSE+NL TAMEEIDEIK +ADDLV       G EGA
Sbjct: 661 GQWPLDLVDGLVDLALGCVTPNGLNSELNLATAMEEIDEIKAKADDLV-------GNEGA 720

Query: 771 DAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 830
           +A NEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIEQWIIAG+DTSPMTNLRLQH
Sbjct: 721 NATNEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGNDTSPMTNLRLQH 780

Query: 831 PYLTPNHTLRSLIQDWQNETSNVYT 847
           PYLTPNHTLRSLIQDWQNETSN YT
Sbjct: 781 PYLTPNHTLRSLIQDWQNETSNRYT 798

BLAST of Spg012119 vs. NCBI nr
Match: KAG7026876.1 (putative U-box domain-containing protein 50, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1345.9 bits (3482), Expect = 0.0e+00
Identity = 694/805 (86.21%), Postives = 744/805 (92.42%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           M GKA+KVYVAIGNDLHDGFKTLGWTL KWK+ PISIVILH+AYT          GKLPA
Sbjct: 1   MTGKADKVYVAIGNDLHDGFKTLGWTLGKWKTQPISIVILHIAYTNSQEYVYTPFGKLPA 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKV+VLR+YEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEP+++ LVDLISELGI
Sbjct: 61  SSVSEEKVKVLRKYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPIHRFLVDLISELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKA+ CELFVIWGGKQVFLRDERIMEDDKGV
Sbjct: 121 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKANSCELFVIWGGKQVFLRDERIMEDDKGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KISSK S K WLG++F+EDP Y +ER LSLSS+SPSSSNS SSRNYWD+N+HELENY
Sbjct: 181 RISKISSKHSFKDWLGKMFMEDPIYSTERMLSLSSSSPSSSNSLSSRNYWDNNIHELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           YEELLSLNLQEE+DCEQDQ+  LE+S STQFN+LDHPDS+MN EE IEHLRTKIE+ARKT
Sbjct: 241 YEELLSLNLQEEKDCEQDQEDVLETSSSTQFNILDHPDSDMNTEESIEHLRTKIEEARKT 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVT+REEL+KELD  KE ILEI
Sbjct: 301 IQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTIREELQKELDPAKECILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK R+SSLLELQAELS KL IST GKSRIEAQLEKTAKTREGMVREIE+LRRQR
Sbjct: 361 VRDIEESKNRISSLLELQAELSRKLQISTAGKSRIEAQLEKTAKTREGMVREIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           DIL+RRIEFCKEKDA GMAERSTEV+CSTRVYTAEEIRLAT+ FSEQMRLSS++YRGRIN
Sbjct: 421 DILNRRIEFCKEKDANGMAERSTEVTCSTRVYTAEEIRLATEGFSEQMRLSSQVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H S+AIQMIDSGN LSEEDFQSKVELLS+IRHPHLIA+MGFCSELKCIVFDYMH GSLSD
Sbjct: 481 HNSIAIQMIDSGNCLSEEDFQSKVELLSHIRHPHLIAMMGFCSELKCIVFDYMHNGSLSD 540

Query: 591 RLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLV 650
           RLLP+N+RSRKI R LMW DRIRIASEVCSGLSFLHQAQPQPISHG+LTLSKILLDQNLV
Sbjct: 541 RLLPTNRRSRKIRRPLMWRDRIRIASEVCSGLSFLHQAQPQPISHGNLTLSKILLDQNLV 600

Query: 651 AKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDDKA 710
           AK+TG GL ELDEAGGIELDIRAFGALLLHLVTGRNW GLIEK  TMGKVG+VQILDD+A
Sbjct: 601 AKVTGFGLAELDEAGGIELDIRAFGALLLHLVTGRNWTGLIEKAPTMGKVGIVQILDDEA 660

Query: 711 GKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGA 770
           G+WPLDLVD LVDLALRCVTPNG NSE+NL TAMEEI+EIK +ADDLV       G EGA
Sbjct: 661 GQWPLDLVDGLVDLALRCVTPNGLNSELNLATAMEEINEIKAKADDLV-------GNEGA 720

Query: 771 DAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 830
           +A NEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH
Sbjct: 721 NATNEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 780

Query: 831 PYLTPNHTLRSLIQDWQNETSNVYT 847
           PYLTPNHTLRSLIQDWQNETSN+YT
Sbjct: 781 PYLTPNHTLRSLIQDWQNETSNLYT 798

BLAST of Spg012119 vs. NCBI nr
Match: KAG6594910.1 (putative U-box domain-containing protein 50, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1344.7 bits (3479), Expect = 0.0e+00
Identity = 694/805 (86.21%), Postives = 744/805 (92.42%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           M GKA+KVYVAIGNDLHDGFKTLGWTL KWK+ PISIVILH+AYT          GKLPA
Sbjct: 1   MTGKADKVYVAIGNDLHDGFKTLGWTLGKWKTQPISIVILHIAYTNSQEYVYTPFGKLPA 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKV+VLR+YEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEP+++ LVDLISELGI
Sbjct: 61  SSVSEEKVKVLRKYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPIHRFLVDLISELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKA+ CELFVIWGGKQVFLRDERIMEDDKGV
Sbjct: 121 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKANSCELFVIWGGKQVFLRDERIMEDDKGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KISSK S K WLG++F+EDP Y +ER LSLSS+SPSSSNS SSRNYWD+N+HELENY
Sbjct: 181 RISKISSKHSFKDWLGKMFMEDPIYSTERMLSLSSSSPSSSNSLSSRNYWDNNIHELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           YEELLSLNLQEE+DCEQDQ+  LE+S STQFN+LDHPDS+MN EE IEHLRTKIE+ARKT
Sbjct: 241 YEELLSLNLQEEKDCEQDQEDVLETSSSTQFNILDHPDSDMNTEESIEHLRTKIEEARKT 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVT+REEL+KELD  KE ILEI
Sbjct: 301 IQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTIREELQKELDPAKECILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLSSLLELQAELS KL IST GKSRIEAQLEKTAKTREGMVREIE+LRRQR
Sbjct: 361 VRDIEESKNRLSSLLELQAELSRKLQISTAGKSRIEAQLEKTAKTREGMVREIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           DIL+RRIEFCKEKDA GMAERSTEV+CSTRVYTAEEIRLAT+ FSEQMRLSS++YRGRIN
Sbjct: 421 DILNRRIEFCKEKDANGMAERSTEVTCSTRVYTAEEIRLATEGFSEQMRLSSQVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H S+AIQMIDSGN LSEEDFQSKVELLS+IRHPHLIA+MGFCSELKCIVFDYMH GSLSD
Sbjct: 481 HNSIAIQMIDSGNCLSEEDFQSKVELLSHIRHPHLIAMMGFCSELKCIVFDYMHNGSLSD 540

Query: 591 RLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLV 650
           RLLP+N+RSRKI R LMW DRIRIASEVCSGLSFLHQAQPQPISHG+LTLSKILLDQNLV
Sbjct: 541 RLLPTNRRSRKIRRPLMWRDRIRIASEVCSGLSFLHQAQPQPISHGNLTLSKILLDQNLV 600

Query: 651 AKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDDKA 710
           AK+TG GL ELDEAGGIELDIRAFGALLLHLVTGRNW  LIEK LTMGKVG+VQILDD+A
Sbjct: 601 AKVTGFGLAELDEAGGIELDIRAFGALLLHLVTGRNWTRLIEKALTMGKVGIVQILDDEA 660

Query: 711 GKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGA 770
           G+WPLDLVD LVDLALRCVTPNG NSE+NL TAME+I+EIK +ADDLV       G EGA
Sbjct: 661 GQWPLDLVDGLVDLALRCVTPNGLNSELNLATAMEKINEIKAKADDLV-------GNEGA 720

Query: 771 DAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 830
           +A NEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH
Sbjct: 721 NATNEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 780

Query: 831 PYLTPNHTLRSLIQDWQNETSNVYT 847
           PYLTPNHTLRSLIQDWQNETSN+YT
Sbjct: 781 PYLTPNHTLRSLIQDWQNETSNLYT 798

BLAST of Spg012119 vs. ExPASy Swiss-Prot
Match: Q9FGD7 (Putative U-box domain-containing protein 50 OS=Arabidopsis thaliana OX=3702 GN=PUB50 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.0e-191
Identity = 385/805 (47.83%), Postives = 523/805 (64.97%), Query Frame = 0

Query: 54  KAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHV-------AYT-IGKLPASSVS 113
           + EKVY+A+GND+ +G+KT+ W L+KW + PISIV+LH+        YT  GKLPASSVS
Sbjct: 18  RMEKVYIAVGNDVQEGYKTIHWALKKWNNIPISIVLLHLCNISQDFVYTPFGKLPASSVS 77

Query: 114 EEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGITNLV 173
           EEK++VLR+YE +KIDKLLSKY+ FCGKV                        L +  ++
Sbjct: 78  EEKLQVLRKYEDQKIDKLLSKYITFCGKV----------------------CPLSVNFVL 137

Query: 174 IGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGVRIAK 233
            G         KSK+AISGSFY+Y+NK  FCE ++I GGK V L+++             
Sbjct: 138 FG---------KSKSAISGSFYVYQNKPEFCEFYIICGGKMVSLKND------------V 197

Query: 234 ISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENYYEEL 293
            ++  +++ W+G++F  DP     RNL  SS +     +S S   WD N+ E+ENY+++L
Sbjct: 198 NNNNSNIRSWIGKMF-HDPG----RNLDRSSGNNDDPTASGSS--WDKNLQEIENYFQQL 257

Query: 294 LSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKTVQLM 353
           LSLNL EEE  E   +   E       NVL H    M+  E++E++R K+ +A+  +   
Sbjct: 258 LSLNLAEEE-TENVVEEEQEDDDDVALNVLQH----MDVAEKLEYVRRKVNEAKLMIDEK 317

Query: 354 KDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEIVGDI 413
             E K +AER  +AEWAI+LC+ R  E EA IKEE   RE+L+  LDS KE I E    +
Sbjct: 318 SREVKVNAERSNRAEWAISLCNSRIGEFEAWIKEESERREKLQATLDSDKECIEEAKNYV 377

Query: 414 EESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQRDILH 473
           E+ K +L SL ELQ  LSSK+      KS+ E +LE+    R  M+ EIE+LR QRD+ +
Sbjct: 378 EKGKTKLHSLAELQEVLSSKVKTMMEAKSQAEVELERVVLQRGEMITEIEKLRSQRDVFN 437

Query: 474 RRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSS-----RMYRGRI 533
           RRIEFCKE++ IG   +  EV C  R Y AE+IRLAT+++S+++RL S      +YRGRI
Sbjct: 438 RRIEFCKEREVIGSVSKE-EVKCGYREYVAEDIRLATETYSDRLRLKSGGNWTNVYRGRI 497

Query: 534 NHTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSEL-KCIVFDYMHGGSL 593
            HT+VA+++I  G+ LS+E F +KV+LL+ IRHP+L+AI GFCS+  KC++F+YMH G+L
Sbjct: 498 KHTTVAVKVI--GDSLSDEAFGAKVKLLNEIRHPNLVAIAGFCSQRPKCLLFEYMHNGNL 557

Query: 594 SDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQN 653
            D L  S ++SR+  + L WHDRIRIA +VCSGL FLH  +P+PI HG LT SKILLD+N
Sbjct: 558 RDNLFTSQRKSRR-SKILKWHDRIRIAHQVCSGLGFLHSVKPKPIVHGRLTPSKILLDRN 617

Query: 654 LVAKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDD 713
           LV KITG GL+   +    + D+ AFG LLLHL+TGRNW GL+ K ++M +  +++ LD 
Sbjct: 618 LVPKITGFGLIMHSDQSDTKPDVMAFGVLLLHLLTGRNWHGLL-KAMSMNQTSILRDLDQ 677

Query: 714 KAGKWPLDLVDALVDLALRCVTPN-GSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGT 773
            AGKWPL+L      LA++C + N G N + + +  MEE+ +I+ +AD+     K  GG 
Sbjct: 678 TAGKWPLELAKEFGALAVKCSSVNRGGNMDFSTKEIMEELGKIREKADEF----KTKGGY 737

Query: 774 EGADAAN---EDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMT 833
           E A  +N    D  D+P +F+CPILQEVMKNPHVAADGFSYELEAI++W+  GHDTSPMT
Sbjct: 738 EEATNSNMDEGDPNDIPSVFMCPILQEVMKNPHVAADGFSYELEAIQEWLSMGHDTSPMT 758

Query: 834 NLRLQHPYLTPNHTLRSLIQDWQNE 841
           NLRL +  LTPNHTLRSLIQDW ++
Sbjct: 798 NLRLDYQMLTPNHTLRSLIQDWHSK 758

BLAST of Spg012119 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 280.8 bits (717), Expect = 5.1e-74
Identity = 250/833 (30.01%), Postives = 388/833 (46.58%), Query Frame = 0

Query: 56  EKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILH-----VAYTIGKLPASSVSEEKVE 115
           EK++VA+   +     TL W L+      I ++ +H     +     K P  +V EE+V 
Sbjct: 45  EKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEVR 104

Query: 116 VLRRYEQEKIDKLLSKYVDFCGK--VKAEILKVEKSDEPVNKLLVDLISELGITNLVIGF 175
           V R  E+EK+  +L  Y+  C +  V+AE + +E   E +   +V LISELGI  LV+G 
Sbjct: 105 VFREKEREKVHMILDDYLRICQQRGVRAEKMFIEM--ESIENGIVQLISELGIRKLVMGA 164

Query: 176 T-----FMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGVRI 235
                   + +  KS+ AI    ++ R     C+++    G  +  R E  M+D +    
Sbjct: 165 AADRHYSRRMTDLKSRKAI----FVRREAPTLCQIWFTCKGYLIHTR-EATMDDTES--- 224

Query: 236 AKISSKPSLKGW-LGRIF--VEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELEN 295
              S +PS+    L + F   E  + +  R  S  S     SN SS+       V +   
Sbjct: 225 EYASPRPSISASDLLQTFSTPESEHQHISRVQSTDSVQQLVSNGSSTEQ--SGRVSD--- 284

Query: 296 YYEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARK 355
                 SLN  EE   E++ DG  E +GS       H   +   +   +    KI  A  
Sbjct: 285 -----GSLNTDEE---ERESDGS-EVTGSATVMSSGHSSPSSFPDGVDDSFNVKIRKATS 344

Query: 356 TVQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILE 415
                K EA     R+ KAE       +R ++ E+   EE+  R++ +  +   KE  + 
Sbjct: 345 EAHSSKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEELKRRKDTEIAVAKEKERFIT 404

Query: 416 IVGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQ 475
           I  + E     L S +  +A L S++  S     ++  +L+   K  + +  E E+L+ +
Sbjct: 405 IKNEQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIAVKLLQKLRDEREELQTE 464

Query: 476 RDILHRRIEFCK---EKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLS----S 535
           RD   R  E  +   E   + + +  T+ S S       EI  AT+ F   +++      
Sbjct: 465 RDRALREAEELRSHAETSTLQLPQYFTDFSFS-------EIEEATNHFDSTLKIGEGGYG 524

Query: 536 RMYRGRINHTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDY 595
            +Y G + HT VAI+M++  +     ++Q +V++LS +RHP++I ++G C E   +V++Y
Sbjct: 525 SIYVGLLRHTQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLIGACPEGWSLVYEY 584

Query: 596 MHGGSLSDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSK 655
           + GGSL DRL   +         L W +R+RIA+E+C+ L FLH  +   + HG L  + 
Sbjct: 585 LPGGSLEDRLTCKDNSP-----PLSWQNRVRIATEICAALVFLHSNKAHSLVHGDLKPAN 644

Query: 656 ILLDQNLVAKIT-----------GSGLVELDEAGGI---------------ELDIRAFGA 715
           ILLD NLV+K++           GS  V  D  G +               + D+ +FG 
Sbjct: 645 ILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTPKSDVYSFGI 704

Query: 716 LLLHLVTGR---NWEGLIEKGLTMGKVGLVQILDDKAGKWPLDLVDALVDLALRCVTPNG 775
           +LL L+TGR        ++  L  G   L  +LD  AG WP    + L  LALRC     
Sbjct: 705 ILLRLLTGRPALRISNEVKYALDNGT--LNDLLDPLAGDWPFVQAEQLARLALRC-CETV 764

Query: 776 SNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGADAANEDVEDVPRLFICPILQEVM 835
           S +  +L T +  + E  R +         +GG+        +    P  FICPI QEVM
Sbjct: 765 SENRPDLGTEVWRVLEPMRAS---------SGGSSSFHLGRNEHRIAPPYFICPIFQEVM 824

Query: 836 KNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLRSLIQDW 838
           ++PHVAADGF+YE EAI  W+ + HDTSPMTN++L H  L  NH LRS IQ+W
Sbjct: 825 QDPHVAADGFTYEAEAIRAWLDSEHDTSPMTNVKLSHTSLIANHALRSAIQEW 829

BLAST of Spg012119 vs. ExPASy Swiss-Prot
Match: Q94A51 (U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.3e-66
Identity = 234/838 (27.92%), Postives = 381/838 (45.47%), Query Frame = 0

Query: 56  EKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILH-----VAYTIGKLPASSVSEEKVE 115
           E ++VA+  D+     T+ W  R +    I ++ +H      ++T  KL   S  +  V+
Sbjct: 15  ETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDVK 74

Query: 116 VLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGITNLVIGFTF 175
           V+ R E+ K+D+L++ Y+    + + +  K+  + + + + +V+LI+   I  LV+G   
Sbjct: 75  VIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAAS 134

Query: 176 MKSSSWKSKNAIS-GSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGVRIAKISSK 235
            K  SWK  +  S  + ++ +     C ++ +  G  +F    R   DD   R     + 
Sbjct: 135 DKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIF---TRASNDDSNNR----QTM 194

Query: 236 PSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENYYEELLSLN 295
           P L                  N +  S    SS       YW S + +     +      
Sbjct: 195 PPLV------------QLDSDNETRKSEKLESSYMRRRLRYWRSLLEQ-----DGEKDTG 254

Query: 296 LQEEEDCEQDQDGGLESSGSTQFNVLDHPD--------------SNMNAEERIEHLRTKI 355
             E E  E        S  S+ F     P+              SN+  +ER   +  K+
Sbjct: 255 QLEREKVEPRAPPLFSSGSSSSFGEPVGPEPVSPELVDSDTLNTSNVEEKEREGDVARKV 314

Query: 356 EDARKTVQLMKDEAK---GSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELD 415
               K +  +    +   G A ++ + + +      + + LE    +E + R+ L++ L+
Sbjct: 315 HRYDKAMHDIGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLE 374

Query: 416 STKEYILEIVGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAK--TREGM 475
             K   LE+   IE++      + ELQ      L + +  +   + + E   K  T   +
Sbjct: 375 KEK---LEVKMVIEQNN---GFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMEL 434

Query: 476 VREIEQLRRQRDILHRRIEFCKEKDAI-GMAERSTEVSCSTRV--YTAEEIRLATDSFSE 535
           ++   Q R +  I H      KE +A+  + +  T  S  + +  Y+  EI  AT+ F  
Sbjct: 435 LKSFRQKRDEIRIDHENA--VKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDP 494

Query: 536 QMRLS----SRMYRGRINHTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFC 595
             +L       +Y+G + H  VA++M+ S   L+  +F+ +VE+LS +RHP+L+ +MG C
Sbjct: 495 SWKLGEGKYGSVYKGNLQHLQVAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGAC 554

Query: 596 SELKCIVFDYMHGGSLSDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQP 655
            E + +++ Y+  GSL D     N        +L W  RIRIASE+CS L FLH   P  
Sbjct: 555 PESRSLIYQYIPNGSLEDCFSSENN-----VPALSWESRIRIASEICSALLFLHSNIP-C 614

Query: 656 ISHGSLTLSKILLDQNLVAKITGSGLVELDEAGG-------------------IELDIRA 715
           I HG+L  SKILLD NLV KI   G+ +L    G                   +E DI A
Sbjct: 615 IIHGNLKPSKILLDSNLVTKINDYGISQLIPIDGLDKSDPHVDPHYFVSREMTLESDIYA 674

Query: 716 FGALLLHLVTGRNWEGLI-EKGLTMGKVGLVQILDDKAGKWPLDLVDALVDLALRCVTPN 775
           FG +LL L+T R   G++ +    +    +  +LD+ AG WP+     L ++A+RC   N
Sbjct: 675 FGIILLQLLTRRPVSGILRDVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKN 734

Query: 776 GSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGADAANEDV-EDVPRLFICPILQE 835
             N   +L   +  ID +K               +E +  AN++V    P  ++CPI QE
Sbjct: 735 PMN-RPDLAVVLRFIDRMK---------APEVPSSETSSYANQNVPRRPPSHYLCPIFQE 794

Query: 836 VMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLRSLIQDWQNE 841
           VMK+P +AADGF+YE EAI +W+  GHDTSPMTNL+++   L PNH L   IQDWQN+
Sbjct: 795 VMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of Spg012119 vs. ExPASy Swiss-Prot
Match: Q5WA76 (U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB70 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 3.0e-58
Identity = 166/551 (30.13%), Postives = 279/551 (50.64%), Query Frame = 0

Query: 329 EHLRTKIEDARKTVQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELK 388
           + L  K++ +     ++K EA  S ER  + E         +E L   +++    + + +
Sbjct: 293 DDLCNKLQKSMDEAAVLKKEA--SDERLKRIE---------SERLARTLEDLYLSQVQQR 352

Query: 389 KELDSTKEYILEIVGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTRE 448
           KE   T+E +  +  + E+ KI+       Q E++ +L         +  QL  + +  E
Sbjct: 353 KE---TEESLSRVQQEFEQLKIQ-------QDEVTVELQRVNEQNENLLGQLSDSREHFE 412

Query: 449 GMVREIEQLRRQRDILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQ 508
            ++ E +QL R+RD   R +E  ++K    ++   T + C    +++ E+  AT++FS  
Sbjct: 413 WLLSEHDQLLRERDNAVREVEELRQKRGQMLSVLVTAMHCE---FSSSEVESATENFSNS 472

Query: 509 MRLSSR----MYRGRINHTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCS 568
           +++       +Y+G + + +VAI+++   +   +  F+ +V +LS +RHPHL+ ++G CS
Sbjct: 473 LKIGEGGFGCVYKGILRNMTVAIKVLRPDSLQGQSQFEQEVSILSRVRHPHLVTLLGACS 532

Query: 569 ELKCIVFDYMHGGSLSDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPI 628
           E   +V++++  GSL D L+ S+KR     ++L W  RIRI +E+CS L FLH+ +P P+
Sbjct: 533 ESSTLVYEFLPNGSLEDFLMCSDKR-----QTLTWQARIRIIAEICSALIFLHKNKPHPV 592

Query: 629 SHGSLTLSKILLDQNLVAKITGSGL----------------------------VELDEAG 688
            HG L  + ILL  NLV+K++  G+                             E    G
Sbjct: 593 VHGDLKPANILLGVNLVSKLSDFGISRLLIQSSTNNTTLYRTMHPVGTPLYMDPEFLSTG 652

Query: 689 GI--ELDIRAFGALLLHLVTGR---NWEGLIEKGLTMGKVGLVQILDDKAGKWPLDLVDA 748
            +  + D+ +FG ++L L+TG+     + ++E  +  G   L  ++D   G+WP   ++ 
Sbjct: 653 ELTPQSDVYSFGIVVLRLLTGKPPVGIKNIVEDAMEKG--DLNSVIDTSVGEWPHLHIEQ 712

Query: 749 LVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGADAANEDVEDV 808
           L  LALRC T        +L   +  I E  R A         A  +  +  + +D    
Sbjct: 713 LAYLALRC-TELSRRCRPDLSGEVWAIVEAIRDA---------ALSSPSSSRSAQDQNSP 772

Query: 809 PRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLR 843
           P  FICPI Q++M +PH+AADGF+YE EAI  W+  GHDTSPMTNL L+H  L PN  LR
Sbjct: 773 PSYFICPISQDIMDDPHIAADGFTYEAEAIRSWLCNGHDTSPMTNLLLEHEELIPNRALR 802

BLAST of Spg012119 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 1.1e-49
Identity = 233/882 (26.42%), Postives = 382/882 (43.31%), Query Frame = 0

Query: 31  PLATRLQRHNRGSSLVDNQSMNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHP-ISIVI 90
           P A R Q   R      ++ M      V +AI   +      + W L K+ S   ++  +
Sbjct: 12  PKAVRAQLPKRADLAAPSEPMT-----VALAISGSIKSK-NVIKWALNKFGSDKNVTFKL 71

Query: 91  LHVAYTIGKLPAS-----SVSEEKVEVLRRYE----QEKIDKLLSKYVDFCGKVKAEI-- 150
           +H+   I  LP +     S+SEE  EV   Y     QE  + LL  +   C + K +I  
Sbjct: 72  IHIHPKITTLPTASGNIVSISEELEEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDE 131

Query: 151 ---------LKVEKSDEPVNKLLVDLISELG---ITNLVIGFTFMKSSSWKSKNAISGSF 210
                    + VE      N + V +  E+    I+NL+IG    +SS   S      + 
Sbjct: 132 TRFESSLTKVAVELQVLESNSVAVAITKEVNQHLISNLIIG----RSSQAASSRNYDITA 191

Query: 211 YIYRNKAHFCELFVIW-GGKQVFLRDERIMEDDKGVRIAKISSKPSLKGWLGRIFVEDPN 270
            I  + ++ C ++V+  GG  +  +D    E           +  S++    R       
Sbjct: 192 SISASVSNLCTVYVVSNGGVHILAKDTSDTE----------RNDTSIESGFER------- 251

Query: 271 YYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENYYEELLSLNLQ------EEEDCEQD 330
                  S S +S S +NS    N   SN H L N   + L   ++      E    E D
Sbjct: 252 ------TSSSCSSGSGANSDVMSNALKSNPHTLSNKRMQNLPTIVRGVSVPMETSSTESD 311

Query: 331 QDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKTVQLMKDEAKGSAERQAKA 390
           +     S  + + +    P+++ +     +    +  D RK       +A  S     + 
Sbjct: 312 ETKKRSSDAAEEASKRSSPETSRSVSWNPQF---RDFDERK-------DAMSSMSSNFEY 371

Query: 391 EWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEIVGDIEESKIRLSSLLELQ 450
              +        + +  + E   +R EL+    + + Y +  V  ++ S+     L EL+
Sbjct: 372 GNVVTPLGHYFTDNQDTLNEISKLRAELR---HAHEMYAVAQVETLDASR----KLNELK 431

Query: 451 AELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQRDILHRRIEFCKEKDAIGM 510
            E  + L   T G ++ E   EK  + R    RE  +  ++R+   +     KEK+ +  
Sbjct: 432 FEELTLLEHETKGIAKKET--EKFEQKR----REEREAAQRREAEMKATHEAKEKEKL-- 491

Query: 511 AERSTEVS--CSTRVYTAEEIRLATDSFSEQMRLS----SRMYRGRINHTSVAIQMIDSG 570
            E S+ V+     + +T EEI  AT SFSE +++       +Y+  ++HT  A++++ S 
Sbjct: 492 -EESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHSA 551

Query: 571 NRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSDRLLPSNKRSRKI 630
                + F  ++E+LS IRHPHL+ ++G C +   +V++YM  GSL DRL   N      
Sbjct: 552 ESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVND----- 611

Query: 631 CRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLVAKITGSGLVELD 690
            + + W  R+RIA EV S L FLH+++P PI H  L  + ILL+ N V+K+   GL  + 
Sbjct: 612 SQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMI 671

Query: 691 EAG--------------------------------GIELDIRAFGALLLHLVTGRNWEGL 750
           +A                                   + D+ AFG ++L L+TG+    L
Sbjct: 672 QAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMAL 731

Query: 751 ---IEKGLTMGKVG-LVQILDDKAGKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEE 810
              +E  +       L+QILD+KAG WP++    L  LAL+C T   S    +L   +  
Sbjct: 732 TYTVETAMENNNDDELIQILDEKAGNWPIEETRQLAALALQC-TELRSKDRPDLEDQILP 791

Query: 811 IDEIKRRADDLVENGKHAGGTEGADAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYE 840
           + E  ++  D   N   A  ++            P  F CP+L++VMK P +AADG++Y+
Sbjct: 792 VLESLKKVADKARNSLSAAPSQ-----------PPSHFFCPLLKDVMKEPCIAADGYTYD 816

BLAST of Spg012119 vs. ExPASy TrEMBL
Match: A0A6J1HEL8 (putative U-box domain-containing protein 50 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463274 PE=4 SV=1)

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 698/805 (86.71%), Postives = 746/805 (92.67%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           M GKA+KVYVAIGNDLHDGFKTLGWTL KWK+ PISIVILH+AYT          GKLPA
Sbjct: 1   MTGKADKVYVAIGNDLHDGFKTLGWTLGKWKTQPISIVILHIAYTNSQEYVYTPFGKLPA 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKV+VLR+YEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEP+++ LVDLISELGI
Sbjct: 61  SSVSEEKVKVLRKYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPIHRFLVDLISELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKA+ CELFVIWGGKQVFLRDERIMEDDKGV
Sbjct: 121 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKANSCELFVIWGGKQVFLRDERIMEDDKGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KISSK S K WLG++F+EDP Y +ER LSLSS+SPSSSNS SSRNYWD+N+HELENY
Sbjct: 181 RISKISSKHSFKDWLGKMFMEDPIYSTERMLSLSSSSPSSSNSLSSRNYWDNNIHELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           YEELLSLNLQEE+DCEQDQ+  LE+S STQFN+LDHPDS+MN EE IEHLRTKIE+ARKT
Sbjct: 241 YEELLSLNLQEEKDCEQDQEDVLETSSSTQFNILDHPDSDMNTEESIEHLRTKIEEARKT 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVT+REEL+KELD  KE ILEI
Sbjct: 301 IQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTIREELQKELDPAKECILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLSSLLELQAELS KL IST GKSRIEAQLEKTAKTREGMVREIE+LRRQR
Sbjct: 361 VRDIEESKNRLSSLLELQAELSRKLQISTAGKSRIEAQLEKTAKTREGMVREIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           DIL+RRIEFCKEKDAIGMAERSTEV+CSTRVYT EEIRLAT+ FSEQMRLSS++YRGRIN
Sbjct: 421 DILNRRIEFCKEKDAIGMAERSTEVTCSTRVYTGEEIRLATEGFSEQMRLSSQVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H S+AIQMIDSGN LSEEDFQSKVELLS+IRHPHLIA+MGFCSELKCIVFDYMH GSLSD
Sbjct: 481 HNSIAIQMIDSGNCLSEEDFQSKVELLSHIRHPHLIAMMGFCSELKCIVFDYMHNGSLSD 540

Query: 591 RLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLV 650
           RLLP+N+RSRKI R LMWHDRIRIASEVCSGLSFLHQAQPQPISHG+LTLSKILLDQNLV
Sbjct: 541 RLLPTNRRSRKIRRPLMWHDRIRIASEVCSGLSFLHQAQPQPISHGNLTLSKILLDQNLV 600

Query: 651 AKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDDKA 710
           AK+TG GL ELDEAGGIELDIRAFGALLLHLVTGRNW GLIEK LTMGKVG+VQILDD+A
Sbjct: 601 AKVTGFGLAELDEAGGIELDIRAFGALLLHLVTGRNWTGLIEKALTMGKVGIVQILDDEA 660

Query: 711 GKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGA 770
           G+WPLDLVD LVDLALRCVTPNG NSE+NL TAMEEIDEIK +ADDLV       G EGA
Sbjct: 661 GQWPLDLVDGLVDLALRCVTPNGLNSELNLATAMEEIDEIKAKADDLV-------GNEGA 720

Query: 771 DAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 830
           +A NEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH
Sbjct: 721 NATNEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 780

Query: 831 PYLTPNHTLRSLIQDWQNETSNVYT 847
           PYLTPNHTLRSLIQDWQNETSN+YT
Sbjct: 781 PYLTPNHTLRSLIQDWQNETSNLYT 798

BLAST of Spg012119 vs. ExPASy TrEMBL
Match: A0A6J1KL40 (putative U-box domain-containing protein 50 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496725 PE=4 SV=1)

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 696/805 (86.46%), Postives = 743/805 (92.30%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           M GKA+KVYVAIGNDLHDGFKTLGWTL KWKS PISIVILH+AYT          GKLPA
Sbjct: 1   MTGKADKVYVAIGNDLHDGFKTLGWTLGKWKSQPISIVILHIAYTNSQEYVYTPFGKLPA 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKV+VLR+YEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEP+++ LVDLISELGI
Sbjct: 61  SSVSEEKVKVLRKYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPIHRFLVDLISELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKA+ CELFVIWGGKQVFLRDERIMEDDKGV
Sbjct: 121 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKANSCELFVIWGGKQVFLRDERIMEDDKGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KIS+K S K WLG++FVEDP Y +ER LSLSS+SPSSSNS SSRNYWD+N+HELENY
Sbjct: 181 RISKISTKHSFKDWLGKMFVEDPIYSTERMLSLSSSSPSSSNSLSSRNYWDNNIHELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           Y+ELLSLNLQEE+DCEQDQ+  LE+S STQFN+LDHPDS+MN EE IEHLRTKIE+ARKT
Sbjct: 241 YKELLSLNLQEEKDCEQDQEDVLETSSSTQFNILDHPDSDMNTEESIEHLRTKIEEARKT 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVT+REEL+KELD  KE ILEI
Sbjct: 301 IQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTIREELQKELDPAKECILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLS LLELQAELS KL IST GKSRIEAQLEKTAKTREGMVREIE+LRRQR
Sbjct: 361 VRDIEESKNRLSPLLELQAELSRKLQISTAGKSRIEAQLEKTAKTREGMVREIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           DIL+RRIEFCKEKDAIGMAERSTEV+CSTRVYTAEEIRLAT+ FSEQMRLSSR+YRGRIN
Sbjct: 421 DILNRRIEFCKEKDAIGMAERSTEVTCSTRVYTAEEIRLATEGFSEQMRLSSRVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H S+AIQMIDSGN LSEEDFQSKVELLS+IRHPHLIA+MGFCSELKCIVFDYMH GSLSD
Sbjct: 481 HNSIAIQMIDSGNCLSEEDFQSKVELLSHIRHPHLIAMMGFCSELKCIVFDYMHSGSLSD 540

Query: 591 RLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLV 650
           RLLP+NKRSRKI R LMWHDRIRIASEVCSGLSFLHQAQPQPISHG+LTLSKILLDQNLV
Sbjct: 541 RLLPTNKRSRKIRRPLMWHDRIRIASEVCSGLSFLHQAQPQPISHGNLTLSKILLDQNLV 600

Query: 651 AKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDDKA 710
           AK+TG GL ELD+AGGIELDIRAFGALLLHLVTGRNW GLIEK LTMGK G+VQILDD+A
Sbjct: 601 AKVTGFGLAELDKAGGIELDIRAFGALLLHLVTGRNWTGLIEKALTMGKAGIVQILDDEA 660

Query: 711 GKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGA 770
           G+WPLDLVD LVDLAL CVTPNG NSE+NL TAMEEIDEIK +ADDLV       G EGA
Sbjct: 661 GQWPLDLVDGLVDLALGCVTPNGLNSELNLATAMEEIDEIKAKADDLV-------GNEGA 720

Query: 771 DAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQH 830
           +A NEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIEQWIIAG+DTSPMTNLRLQH
Sbjct: 721 NATNEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGNDTSPMTNLRLQH 780

Query: 831 PYLTPNHTLRSLIQDWQNETSNVYT 847
           PYLTPNHTLRSLIQDWQNETSN YT
Sbjct: 781 PYLTPNHTLRSLIQDWQNETSNRYT 798

BLAST of Spg012119 vs. ExPASy TrEMBL
Match: A0A6J1HGE5 (putative U-box domain-containing protein 50 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463274 PE=4 SV=1)

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 698/830 (84.10%), Postives = 746/830 (89.88%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           M GKA+KVYVAIGNDLHDGFKTLGWTL KWK+ PISIVILH+AYT          GKLPA
Sbjct: 1   MTGKADKVYVAIGNDLHDGFKTLGWTLGKWKTQPISIVILHIAYTNSQEYVYTPFGKLPA 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKV+VLR+YEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEP+++ LVDLISELGI
Sbjct: 61  SSVSEEKVKVLRKYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPIHRFLVDLISELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKA+ CELFVIWGGKQVFLRDERIMEDDKGV
Sbjct: 121 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKANSCELFVIWGGKQVFLRDERIMEDDKGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KISSK S K WLG++F+EDP Y +ER LSLSS+SPSSSNS SSRNYWD+N+HELENY
Sbjct: 181 RISKISSKHSFKDWLGKMFMEDPIYSTERMLSLSSSSPSSSNSLSSRNYWDNNIHELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           YEELLSLNLQEE+DCEQDQ+  LE+S STQFN+LDHPDS+MN EE IEHLRTKIE+ARKT
Sbjct: 241 YEELLSLNLQEEKDCEQDQEDVLETSSSTQFNILDHPDSDMNTEESIEHLRTKIEEARKT 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVT+REEL+KELD  KE ILEI
Sbjct: 301 IQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTIREELQKELDPAKECILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLSSLLELQAELS KL IST GKSRIEAQLEKTAKTREGMVREIE+LRRQR
Sbjct: 361 VRDIEESKNRLSSLLELQAELSRKLQISTAGKSRIEAQLEKTAKTREGMVREIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           DIL+RRIEFCKEKDAIGMAERSTEV+CSTRVYT EEIRLAT+ FSEQMRLSS++YRGRIN
Sbjct: 421 DILNRRIEFCKEKDAIGMAERSTEVTCSTRVYTGEEIRLATEGFSEQMRLSSQVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSK-------------------------VELLSNIRHPHL 590
           H S+AIQMIDSGN LSEEDFQSK                         VELLS+IRHPHL
Sbjct: 481 HNSIAIQMIDSGNCLSEEDFQSKVADALFSTVFNCQKLCFHPNCVYLQVELLSHIRHPHL 540

Query: 591 IAIMGFCSELKCIVFDYMHGGSLSDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFL 650
           IA+MGFCSELKCIVFDYMH GSLSDRLLP+N+RSRKI R LMWHDRIRIASEVCSGLSFL
Sbjct: 541 IAMMGFCSELKCIVFDYMHNGSLSDRLLPTNRRSRKIRRPLMWHDRIRIASEVCSGLSFL 600

Query: 651 HQAQPQPISHGSLTLSKILLDQNLVAKITGSGLVELDEAGGIELDIRAFGALLLHLVTGR 710
           HQAQPQPISHG+LTLSKILLDQNLVAK+TG GL ELDEAGGIELDIRAFGALLLHLVTGR
Sbjct: 601 HQAQPQPISHGNLTLSKILLDQNLVAKVTGFGLAELDEAGGIELDIRAFGALLLHLVTGR 660

Query: 711 NWEGLIEKGLTMGKVGLVQILDDKAGKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAME 770
           NW GLIEK LTMGKVG+VQILDD+AG+WPLDLVD LVDLALRCVTPNG NSE+NL TAME
Sbjct: 661 NWTGLIEKALTMGKVGIVQILDDEAGQWPLDLVDGLVDLALRCVTPNGLNSELNLATAME 720

Query: 771 EIDEIKRRADDLVENGKHAGGTEGADAANEDVEDVPRLFICPILQEVMKNPHVAADGFSY 830
           EIDEIK +ADDLV       G EGA+A NEDV+DVPR+FICPILQEVMKNPHVAADGFSY
Sbjct: 721 EIDEIKAKADDLV-------GNEGANATNEDVDDVPRIFICPILQEVMKNPHVAADGFSY 780

Query: 831 ELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLRSLIQDWQNETSNVYT 847
           ELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLRSLIQDWQNETSN+YT
Sbjct: 781 ELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLRSLIQDWQNETSNLYT 823

BLAST of Spg012119 vs. ExPASy TrEMBL
Match: A0A5D3CLH3 (Putative U-box domain-containing protein 50 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003310 PE=4 SV=1)

HSP 1 Score: 1302.0 bits (3368), Expect = 0.0e+00
Identity = 669/806 (83.00%), Postives = 734/806 (91.07%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           MN K EKV+VAIGNDL DGFKTLGWTLRKWKSHPISIVILHV+Y           GKLP 
Sbjct: 1   MNAKEEKVFVAIGNDLRDGFKTLGWTLRKWKSHPISIVILHVSYNNSMEYVHTPFGKLPV 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKVEVLRRYEQEKI+K+LSKYV+FCGKV+AEILKVEKSD+PV+KL+VDL+SELGI
Sbjct: 61  SSVSEEKVEVLRRYEQEKINKILSKYVEFCGKVRAEILKVEKSDKPVHKLIVDLVSELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWK KNAISGSFYIYRNKAHFCEL+VIWGGKQVFLRDERIMED+ GV
Sbjct: 121 TNLVIGFTFMKSSSWKPKNAISGSFYIYRNKAHFCELYVIWGGKQVFLRDERIMEDESGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KIS+K SLKGWLG++FVEDP Y+S+RNLSLSS+SP SSNS SS+NYWD NV ELENY
Sbjct: 181 RISKISTKHSLKGWLGKMFVEDPTYFSDRNLSLSSSSPHSSNSMSSQNYWDHNVQELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           YEELLSLN+QEEEDCEQDQDG LE+S ST+FN+LD+PDSNMN EERIEHLRTKIE+ARK+
Sbjct: 241 YEELLSLNVQEEEDCEQDQDGVLENSSSTRFNILDYPDSNMNPEERIEHLRTKIEEARKS 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLM+DEAKGS+ERQAKAEWAINLCSQRT+ELEA+IKEEVT+RE+L+KELDS KEYILEI
Sbjct: 301 IQLMRDEAKGSSERQAKAEWAINLCSQRTDELEAKIKEEVTIREDLQKELDSAKEYILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLSSLL+LQAELSSKL IST  KSR+EAQLEKTAKT++GMVR IE+LRRQR
Sbjct: 361 VADIEESKTRLSSLLKLQAELSSKLQISTAEKSRLEAQLEKTAKTKKGMVRAIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           +ILHRRIEFCK++DAIGM ERS EVSCSTRVYT EEI LATD+FSEQMRLSSR+YRGRIN
Sbjct: 421 EILHRRIEFCKDRDAIGMGERSIEVSCSTRVYTVEEITLATDNFSEQMRLSSRVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H SVAIQMI SGNRLSE+DFQSKVELLS+I HPHLIA++GFC ELKCIVFDYMH GSLSD
Sbjct: 481 HLSVAIQMITSGNRLSEDDFQSKVELLSHIHHPHLIAMIGFCPELKCIVFDYMHSGSLSD 540

Query: 591 RLLP--SNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQN 650
           RLLP  SNKRS+KICR LMW +RIRIASEVCSGLSFLHQAQPQPISHG LTLSKILLDQN
Sbjct: 541 RLLPSNSNKRSKKICRPLMWSERIRIASEVCSGLSFLHQAQPQPISHGKLTLSKILLDQN 600

Query: 651 LVAKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDD 710
           L AK+TG GL ELDE  GIELDIRAFGALLLH+VTGR   G IE+ L+MGKVGLVQILD+
Sbjct: 601 LAAKVTGFGLDELDEGRGIELDIRAFGALLLHIVTGRTGAGQIEEVLSMGKVGLVQILDE 660

Query: 711 KAGKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDL-VENGKHAGGT 770
           KAG+WPL LVD L+DLALRC TPNGS+S+V L T MEEIDEIKR+ADDL V NGK     
Sbjct: 661 KAGQWPLSLVDHLLDLALRCATPNGSSSDVKLGTVMEEIDEIKRKADDLVVGNGKKVEVI 720

Query: 771 EGADAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLR 830
           EGADAANEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIEQWI AGH+TSPMTNL+
Sbjct: 721 EGADAANEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEQWIGAGHETSPMTNLK 780

Query: 831 LQHPYLTPNHTLRSLIQDWQNETSNV 845
           LQHPYLTPNHTLRSLIQDWQNE SNV
Sbjct: 781 LQHPYLTPNHTLRSLIQDWQNENSNV 806

BLAST of Spg012119 vs. ExPASy TrEMBL
Match: A0A5A7T4P4 (Putative U-box domain-containing protein 50 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G001480 PE=4 SV=1)

HSP 1 Score: 1300.8 bits (3365), Expect = 0.0e+00
Identity = 668/806 (82.88%), Postives = 734/806 (91.07%), Query Frame = 0

Query: 51  MNGKAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHVAYT---------IGKLPA 110
           MN K EKV+VAIGNDL DGFKTLGWTLRKWKSHPISIVILHV+Y           GKLP 
Sbjct: 1   MNAKEEKVFVAIGNDLRDGFKTLGWTLRKWKSHPISIVILHVSYNNSMEYVHTPFGKLPV 60

Query: 111 SSVSEEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGI 170
           SSVSEEKVEVLRRYEQEKI+K+LSKYVDFCGKV+AEILKVEKSD+PV+KL+VDL+SELGI
Sbjct: 61  SSVSEEKVEVLRRYEQEKINKILSKYVDFCGKVRAEILKVEKSDKPVHKLIVDLVSELGI 120

Query: 171 TNLVIGFTFMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGV 230
           TNLVIGFTFMKSSSWK KNAISGSFYIYRNKAHFCEL+VIWGGKQVFLRDERIMED+ GV
Sbjct: 121 TNLVIGFTFMKSSSWKPKNAISGSFYIYRNKAHFCELYVIWGGKQVFLRDERIMEDESGV 180

Query: 231 RIAKISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENY 290
           RI+KIS+K SLKGWLG++FVEDP Y+S+RNLSLSS+SP SSNS SS+NYWD NV ELENY
Sbjct: 181 RISKISTKHSLKGWLGKMFVEDPTYFSDRNLSLSSSSPHSSNSMSSQNYWDHNVQELENY 240

Query: 291 YEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 350
           YEELLSLN+QEEEDCEQDQDG LE+S ST+FN+LD+PDSNMN EERIEHLRTKIE+ARK+
Sbjct: 241 YEELLSLNVQEEEDCEQDQDGVLENSSSTRFNILDYPDSNMNPEERIEHLRTKIEEARKS 300

Query: 351 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 410
           +QLM+DEAKGS+ERQAKAEWAINLCSQRT+ELEA+IKEEVT+RE+L+KELDS KEYILEI
Sbjct: 301 IQLMRDEAKGSSERQAKAEWAINLCSQRTDELEAKIKEEVTIREDLQKELDSAKEYILEI 360

Query: 411 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 470
           V DIEESK RLSSLL+LQAELSSKL IST  KSR+EAQLEKTAKT++G+VR IE+LRRQR
Sbjct: 361 VADIEESKTRLSSLLKLQAELSSKLQISTAEKSRLEAQLEKTAKTKKGLVRAIEELRRQR 420

Query: 471 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSSRMYRGRIN 530
           +ILHRRIEFCK++DA+GM ERS EVSCSTRVYT EEI LATD+FSEQMRLSSR+YRGRIN
Sbjct: 421 EILHRRIEFCKDRDAMGMGERSIEVSCSTRVYTVEEITLATDNFSEQMRLSSRVYRGRIN 480

Query: 531 HTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSD 590
           H SVAIQMI SGNRLSE+DFQSKVELLS+I HPHLIA++GFC ELKCIVFDYMH GSLSD
Sbjct: 481 HLSVAIQMITSGNRLSEDDFQSKVELLSHIHHPHLIAMIGFCPELKCIVFDYMHSGSLSD 540

Query: 591 RLLP--SNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQN 650
           RLLP  SNKRS+KICR LMW +RIRIASEVCSGLSFLHQAQPQPISHG LTLSKILLDQN
Sbjct: 541 RLLPSNSNKRSKKICRPLMWSERIRIASEVCSGLSFLHQAQPQPISHGKLTLSKILLDQN 600

Query: 651 LVAKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQILDD 710
           L AK+TG GL ELDE  GIELDIRAFGALLLH+VTGR   G IE+ L+MGKVGLVQILD+
Sbjct: 601 LAAKVTGFGLDELDEGRGIELDIRAFGALLLHIVTGRTEAGQIEEVLSMGKVGLVQILDE 660

Query: 711 KAGKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEIDEIKRRADDL-VENGKHAGGT 770
           KAG+WPL LVD L+DLALRC TPNGS+S+V L T MEEIDEIKR+ADDL V NGK     
Sbjct: 661 KAGQWPLSLVDDLLDLALRCATPNGSSSDVKLGTVMEEIDEIKRKADDLVVGNGKKVEVI 720

Query: 771 EGADAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLR 830
           EGADAANEDV+DVPR+FICPILQEVMKNPHVAADGFSYELEAIEQWI AGH+TSPMTNL+
Sbjct: 721 EGADAANEDVDDVPRIFICPILQEVMKNPHVAADGFSYELEAIEQWIGAGHETSPMTNLK 780

Query: 831 LQHPYLTPNHTLRSLIQDWQNETSNV 845
           LQHPYLTPNHTLRSLIQDWQNE SNV
Sbjct: 781 LQHPYLTPNHTLRSLIQDWQNENSNV 806

BLAST of Spg012119 vs. TAIR 10
Match: AT5G65500.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 719.5 bits (1856), Expect = 3.1e-207
Identity = 404/809 (49.94%), Postives = 548/809 (67.74%), Query Frame = 0

Query: 54  KAEKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILHV-------AYT-IGKLPASSVS 113
           + EKVY+A+GND+ +G+KT+ W L+KW + PISIV+LH+        YT  GKLPASSVS
Sbjct: 18  RMEKVYIAVGNDVQEGYKTIHWALKKWNNIPISIVLLHLCNISQDFVYTPFGKLPASSVS 77

Query: 114 EEKVEVLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGITNLV 173
           EEK++VLR+YE +KIDKLLSKY+ FCGKVKAE+LKVEK  + +  L++DLIS+L IT LV
Sbjct: 78  EEKLQVLRKYEDQKIDKLLSKYITFCGKVKAELLKVEKQHDSIQVLILDLISKLRITKLV 137

Query: 174 IGFTFMK-SSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGVRIA 233
           +G TFM+ SSSWKSK+AISGSFY+Y+NK  FCE ++I GGK V L+++            
Sbjct: 138 MGITFMRSSSSWKSKSAISGSFYVYQNKPEFCEFYIICGGKMVSLKND------------ 197

Query: 234 KISSKPSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENYYEE 293
             ++  +++ W+G++F  DP     RNL  SS +     +S S   WD N+ E+ENY+++
Sbjct: 198 VNNNNSNIRSWIGKMF-HDPG----RNLDRSSGNNDDPTASGSS--WDKNLQEIENYFQQ 257

Query: 294 LLSLNLQEEED---CEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARKT 353
           LLSLNL EEE     E++Q+              D  D  +N  + +  +  K+ +A+  
Sbjct: 258 LLSLNLAEEETENVVEEEQE--------------DDDDVALNVLQHMVKILRKVNEAKLM 317

Query: 354 VQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEI 413
           +     E K +AER  +AEWAI+LC+ R  E EA IKEE   RE+L+  LDS KE I E 
Sbjct: 318 IDEKSREVKVNAERSNRAEWAISLCNSRIGEFEAWIKEESERREKLQATLDSDKECIEEA 377

Query: 414 VGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQR 473
              +E+ K +L SL ELQ  LSSK+      KS+ E +LE+    R  M+ EIE+LR QR
Sbjct: 378 KNYVEKGKTKLHSLAELQEVLSSKVKTMMEAKSQAEVELERVVLQRGEMITEIEKLRSQR 437

Query: 474 DILHRRIEFCKEKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLSS-----RMY 533
           D+ +RRIEFCKE++ IG   +  EV C  R Y AE+IRLAT+++S+++RL S      +Y
Sbjct: 438 DVFNRRIEFCKEREVIGSVSKE-EVKCGYREYVAEDIRLATETYSDRLRLKSGGNWTNVY 497

Query: 534 RGRINHTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSEL-KCIVFDYMH 593
           RGRI HT+VA+++I  G+ LS+E F +KV+LL+ IRHP+L+AI GFCS+  KC++F+YMH
Sbjct: 498 RGRIKHTTVAVKVI--GDSLSDEAFGAKVKLLNEIRHPNLVAIAGFCSQRPKCLLFEYMH 557

Query: 594 GGSLSDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKIL 653
            G+L D L  S ++SR+  + L WHDRIRIA +VCSGL FLH  +P+PI HG LT SKIL
Sbjct: 558 NGNLRDNLFTSQRKSRR-SKILKWHDRIRIAHQVCSGLGFLHSVKPKPIVHGRLTPSKIL 617

Query: 654 LDQNLVAKITGSGLVELDEAGGIELDIRAFGALLLHLVTGRNWEGLIEKGLTMGKVGLVQ 713
           LD+NLV KITG GL+   +    + D+ AFG LLLHL+TGRNW GL+ K ++M +  +++
Sbjct: 618 LDRNLVPKITGFGLIMHSDQSDTKPDVMAFGVLLLHLLTGRNWHGLL-KAMSMNQTSILR 677

Query: 714 ILDDKAGKWPLDLVDALVDLALRCVTPN-GSNSEVNLRTAMEEIDEIKRRADDLVENGKH 773
            LD  AGKWPL+L      LA++C + N G N + + +  MEE+ +I+ +AD+     K 
Sbjct: 678 DLDQTAGKWPLELAKEFGALAVKCSSVNRGGNMDFSTKEIMEELGKIREKADEF----KT 737

Query: 774 AGGTEGADAAN---EDVEDVPRLFICPILQEVMKNPHVAADGFSYELEAIEQWIIAGHDT 833
            GG E A  +N    D  D+P +F+CPILQEVMKNPHVAADGFSYELEAI++W+  GHDT
Sbjct: 738 KGGYEEATNSNMDEGDPNDIPSVFMCPILQEVMKNPHVAADGFSYELEAIQEWLSMGHDT 784

Query: 834 SPMTNLRLQHPYLTPNHTLRSLIQDWQNE 841
           SPMTNLRL +  LTPNHTLRSLIQDW ++
Sbjct: 798 SPMTNLRLDYQMLTPNHTLRSLIQDWHSK 784

BLAST of Spg012119 vs. TAIR 10
Match: AT2G45910.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 280.8 bits (717), Expect = 3.6e-75
Identity = 250/833 (30.01%), Postives = 388/833 (46.58%), Query Frame = 0

Query: 56  EKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILH-----VAYTIGKLPASSVSEEKVE 115
           EK++VA+   +     TL W L+      I ++ +H     +     K P  +V EE+V 
Sbjct: 45  EKIFVAVDKHVAKSKSTLIWALQNTGGKKICLIHVHQPSQMIPLMGAKFPVGAVKEEEVR 104

Query: 116 VLRRYEQEKIDKLLSKYVDFCGK--VKAEILKVEKSDEPVNKLLVDLISELGITNLVIGF 175
           V R  E+EK+  +L  Y+  C +  V+AE + +E   E +   +V LISELGI  LV+G 
Sbjct: 105 VFREKEREKVHMILDDYLRICQQRGVRAEKMFIEM--ESIENGIVQLISELGIRKLVMGA 164

Query: 176 T-----FMKSSSWKSKNAISGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGVRI 235
                   + +  KS+ AI    ++ R     C+++    G  +  R E  M+D +    
Sbjct: 165 AADRHYSRRMTDLKSRKAI----FVRREAPTLCQIWFTCKGYLIHTR-EATMDDTES--- 224

Query: 236 AKISSKPSLKGW-LGRIF--VEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELEN 295
              S +PS+    L + F   E  + +  R  S  S     SN SS+       V +   
Sbjct: 225 EYASPRPSISASDLLQTFSTPESEHQHISRVQSTDSVQQLVSNGSSTEQ--SGRVSD--- 284

Query: 296 YYEELLSLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPDSNMNAEERIEHLRTKIEDARK 355
                 SLN  EE   E++ DG  E +GS       H   +   +   +    KI  A  
Sbjct: 285 -----GSLNTDEE---ERESDGS-EVTGSATVMSSGHSSPSSFPDGVDDSFNVKIRKATS 344

Query: 356 TVQLMKDEAKGSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILE 415
                K EA     R+ KAE       +R ++ E+   EE+  R++ +  +   KE  + 
Sbjct: 345 EAHSSKQEAFAETLRRQKAEKNALDAIRRAKQSESAYSEELKRRKDTEIAVAKEKERFIT 404

Query: 416 IVGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVREIEQLRRQ 475
           I  + E     L S +  +A L S++  S     ++  +L+   K  + +  E E+L+ +
Sbjct: 405 IKNEQEVIMEELQSAMAQKAMLESQIAKSDGTMEKLNQKLDIAVKLLQKLRDEREELQTE 464

Query: 476 RDILHRRIEFCK---EKDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLS----S 535
           RD   R  E  +   E   + + +  T+ S S       EI  AT+ F   +++      
Sbjct: 465 RDRALREAEELRSHAETSTLQLPQYFTDFSFS-------EIEEATNHFDSTLKIGEGGYG 524

Query: 536 RMYRGRINHTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDY 595
            +Y G + HT VAI+M++  +     ++Q +V++LS +RHP++I ++G C E   +V++Y
Sbjct: 525 SIYVGLLRHTQVAIKMLNPNSSQGPVEYQQEVDVLSKMRHPNIITLIGACPEGWSLVYEY 584

Query: 596 MHGGSLSDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSK 655
           + GGSL DRL   +         L W +R+RIA+E+C+ L FLH  +   + HG L  + 
Sbjct: 585 LPGGSLEDRLTCKDNSP-----PLSWQNRVRIATEICAALVFLHSNKAHSLVHGDLKPAN 644

Query: 656 ILLDQNLVAKIT-----------GSGLVELDEAGGI---------------ELDIRAFGA 715
           ILLD NLV+K++           GS  V  D  G +               + D+ +FG 
Sbjct: 645 ILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTVAYLDPEASSSGELTPKSDVYSFGI 704

Query: 716 LLLHLVTGR---NWEGLIEKGLTMGKVGLVQILDDKAGKWPLDLVDALVDLALRCVTPNG 775
           +LL L+TGR        ++  L  G   L  +LD  AG WP    + L  LALRC     
Sbjct: 705 ILLRLLTGRPALRISNEVKYALDNGT--LNDLLDPLAGDWPFVQAEQLARLALRC-CETV 764

Query: 776 SNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGADAANEDVEDVPRLFICPILQEVM 835
           S +  +L T +  + E  R +         +GG+        +    P  FICPI QEVM
Sbjct: 765 SENRPDLGTEVWRVLEPMRAS---------SGGSSSFHLGRNEHRIAPPYFICPIFQEVM 824

Query: 836 KNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLRSLIQDW 838
           ++PHVAADGF+YE EAI  W+ + HDTSPMTN++L H  L  NH LRS IQ+W
Sbjct: 825 QDPHVAADGFTYEAEAIRAWLDSEHDTSPMTNVKLSHTSLIANHALRSAIQEW 829

BLAST of Spg012119 vs. TAIR 10
Match: AT3G49060.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 256.1 bits (653), Expect = 9.6e-68
Identity = 234/838 (27.92%), Postives = 381/838 (45.47%), Query Frame = 0

Query: 56  EKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILH-----VAYTIGKLPASSVSEEKVE 115
           E ++VA+  D+     T+ W  R +    I ++ +H      ++T  KL   S  +  V+
Sbjct: 15  ETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDVK 74

Query: 116 VLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGITNLVIGFTF 175
           V+ R E+ K+D+L++ Y+    + + +  K+  + + + + +V+LI+   I  LV+G   
Sbjct: 75  VIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAAS 134

Query: 176 MKSSSWKSKNAIS-GSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGVRIAKISSK 235
            K  SWK  +  S  + ++ +     C ++ +  G  +F    R   DD   R     + 
Sbjct: 135 DKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIF---TRASNDDSNNR----QTM 194

Query: 236 PSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENYYEELLSLN 295
           P L                  N +  S    SS       YW S + +     +      
Sbjct: 195 PPLV------------QLDSDNETRKSEKLESSYMRRRLRYWRSLLEQ-----DGEKDTG 254

Query: 296 LQEEEDCEQDQDGGLESSGSTQFNVLDHPD--------------SNMNAEERIEHLRTKI 355
             E E  E        S  S+ F     P+              SN+  +ER   +  K+
Sbjct: 255 QLEREKVEPRAPPLFSSGSSSSFGEPVGPEPVSPELVDSDTLNTSNVEEKEREGDVARKV 314

Query: 356 EDARKTVQLMKDEAK---GSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELD 415
               K +  +    +   G A ++ + + +      + + LE    +E + R+ L++ L+
Sbjct: 315 HRYDKAMHDIGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEELLE 374

Query: 416 STKEYILEIVGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAK--TREGM 475
             K   LE+   IE++      + ELQ      L + +  +   + + E   K  T   +
Sbjct: 375 KEK---LEVKMVIEQNN---GFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTAMEL 434

Query: 476 VREIEQLRRQRDILHRRIEFCKEKDAI-GMAERSTEVSCSTRV--YTAEEIRLATDSFSE 535
           ++   Q R +  I H      KE +A+  + +  T  S  + +  Y+  EI  AT+ F  
Sbjct: 435 LKSFRQKRDEIRIDHENA--VKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNEFDP 494

Query: 536 QMRLS----SRMYRGRINHTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFC 595
             +L       +Y+G + H  VA++M+ S   L+  +F+ +VE+LS +RHP+L+ +MG C
Sbjct: 495 SWKLGEGKYGSVYKGNLQHLQVAVKMLPSYGSLNHFEFERRVEILSRVRHPNLVTLMGAC 554

Query: 596 SELKCIVFDYMHGGSLSDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQP 655
            E + +++ Y+  GSL D     N        +L W  RIRIASE+CS L FLH   P  
Sbjct: 555 PESRSLIYQYIPNGSLEDCFSSENN-----VPALSWESRIRIASEICSALLFLHSNIP-C 614

Query: 656 ISHGSLTLSKILLDQNLVAKITGSGLVELDEAGG-------------------IELDIRA 715
           I HG+L  SKILLD NLV KI   G+ +L    G                   +E DI A
Sbjct: 615 IIHGNLKPSKILLDSNLVTKINDYGISQLIPIDGLDKSDPHVDPHYFVSREMTLESDIYA 674

Query: 716 FGALLLHLVTGRNWEGLI-EKGLTMGKVGLVQILDDKAGKWPLDLVDALVDLALRCVTPN 775
           FG +LL L+T R   G++ +    +    +  +LD+ AG WP+     L ++A+RC   N
Sbjct: 675 FGIILLQLLTRRPVSGILRDVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCCKKN 734

Query: 776 GSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGADAANEDV-EDVPRLFICPILQE 835
             N   +L   +  ID +K               +E +  AN++V    P  ++CPI QE
Sbjct: 735 PMN-RPDLAVVLRFIDRMK---------APEVPSSETSSYANQNVPRRPPSHYLCPIFQE 794

Query: 836 VMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLRSLIQDWQNE 841
           VMK+P +AADGF+YE EAI +W+  GHDTSPMTNL+++   L PNH L   IQDWQN+
Sbjct: 795 VMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAIQDWQNQ 804

BLAST of Spg012119 vs. TAIR 10
Match: AT3G49060.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 240.0 bits (611), Expect = 7.1e-63
Identity = 233/841 (27.71%), Postives = 373/841 (44.35%), Query Frame = 0

Query: 56  EKVYVAIGNDLHDGFKTLGWTLRKWKSHPISIVILH-----VAYTIGKLPASSVSEEKVE 115
           E ++VA+  D+     T+ W  R +    I ++ +H      ++T  KL   S  +  V+
Sbjct: 15  ETIFVAVAEDVERSKTTVLWAARNFSGKKICLLYVHRTARAASWTHKKLVGGSFKKHDVK 74

Query: 116 VLRRYEQEKIDKLLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGITNLVIGFTF 175
           V+ R E+ K+D+L++ Y+    + + +  K+  + + + + +V+LI+   I  LV+G   
Sbjct: 75  VIERVEKPKVDELMNSYLQLLSETEIQTDKLCIAGQNIEECIVELIARHKIKWLVMGAAS 134

Query: 176 MKSSSWKSKNAIS-GSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGVRIAKISSK 235
            K  SWK  +  S  + ++ +     C ++ +  G  +F    R   DD   R     + 
Sbjct: 135 DKHYSWKMTDLKSKKAIFVCKKAPDSCHIWFLCKGYLIF---TRASNDDSNNR----QTM 194

Query: 236 PSLKGWLGRIFVEDPNYYSERNLSLSSASPSSSNSSSSRNYWDS---NVHELENYYEELL 295
           P L                  N +  S    SS       YW S    V+ L+N  +   
Sbjct: 195 PPLV------------QLDSDNETRKSEKLESSYMRRRLRYWRSLLEQVNLLQNCADGEK 254

Query: 296 SLNLQEEEDCEQDQDGGLESSGSTQFNVLDHPD--------------SNMNAEERIEHLR 355
                E E  E        S  S+ F     P+              SN+  +ER   + 
Sbjct: 255 DTGQLEREKVEPRAPPLFSSGSSSSFGEPVGPEPVSPELVDSDTLNTSNVEEKEREGDVA 314

Query: 356 TKIEDARKTVQLMKDEAK---GSAERQAKAEWAINLCSQRTEELEAQIKEEVTVREELKK 415
            K+    K +  +    +   G A ++ + + +      + + LE    +E + R+ L++
Sbjct: 315 RKVHRYDKAMHDIGQSDRTVYGEAGKKWEEDASTTEALCKAKALEGLCIKESSQRKRLEE 374

Query: 416 ELDSTKEYILEIVGDIEESKIRLSSLLELQAELSSKLLISTVGKSRIEAQLEKTAK--TR 475
            L+  K   LE+   IE++      + ELQ      L + +  +   + + E   K  T 
Sbjct: 375 LLEKEK---LEVKMVIEQNN---GFMKELQMVQGRNLKLESQMRKLQDLEKEHGEKFDTA 434

Query: 476 EGMVREIEQLRRQRDILHRRIEFCKEKDAI-GMAERSTEVSCSTRV--YTAEEIRLATDS 535
             +++   Q R +  I H      KE +A+  + +  T  S  + +  Y+  EI  AT+ 
Sbjct: 435 MELLKSFRQKRDEIRIDHENA--VKEVNALRRLVKGETGESSGSEMLDYSFMEINEATNE 494

Query: 536 FSEQMRLS----SRMYRGRINHTSVAIQMIDSGNRLSEEDFQSKVELLSNIRHPHLIAIM 595
           F    +L       +Y+G + H  VA                  VE+LS +RHP+L+ +M
Sbjct: 495 FDPSWKLGEGKYGSVYKGNLQHLQVA------------------VEILSRVRHPNLVTLM 554

Query: 596 GFCSELKCIVFDYMHGGSLSDRLLPSNKRSRKICRSLMWHDRIRIASEVCSGLSFLHQAQ 655
           G C E + +++ Y+  GSL D     N        +L W  RIRIASE+CS L FLH   
Sbjct: 555 GACPESRSLIYQYIPNGSLEDCFSSENN-----VPALSWESRIRIASEICSALLFLHSNI 614

Query: 656 PQPISHGSLTLSKILLDQNLVAKITGSGLVELDEAGG-------------------IELD 715
           P  I HG+L  SKILLD NLV KI   G+ +L    G                   +E D
Sbjct: 615 P-CIIHGNLKPSKILLDSNLVTKINDYGISQLIPIDGLDKSDPHVDPHYFVSREMTLESD 674

Query: 716 IRAFGALLLHLVTGRNWEGLI-EKGLTMGKVGLVQILDDKAGKWPLDLVDALVDLALRCV 775
           I AFG +LL L+T R   G++ +    +    +  +LD+ AG WP+     L ++A+RC 
Sbjct: 675 IYAFGIILLQLLTRRPVSGILRDVKCALENDNISAVLDNSAGDWPVARGKKLANVAIRCC 734

Query: 776 TPNGSNSEVNLRTAMEEIDEIKRRADDLVENGKHAGGTEGADAANEDV-EDVPRLFICPI 835
             N  N   +L   +  ID +K               +E +  AN++V    P  ++CPI
Sbjct: 735 KKNPMN-RPDLAVVLRFIDRMK---------APEVPSSETSSYANQNVPRRPPSHYLCPI 794

Query: 836 LQEVMKNPHVAADGFSYELEAIEQWIIAGHDTSPMTNLRLQHPYLTPNHTLRSLIQDWQN 841
            QEVMK+P +AADGF+YE EAI +W+  GHDTSPMTNL+++   L PNH L   IQDWQN
Sbjct: 795 FQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAIQDWQN 794

BLAST of Spg012119 vs. TAIR 10
Match: AT5G57035.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 217.2 bits (552), Expect = 4.9e-56
Identity = 215/823 (26.12%), Postives = 361/823 (43.86%), Query Frame = 0

Query: 71  KTLGWTLRKWKSHPISIVILHVAYTI--------GKLPASSVSEEKVEVLRRYEQEKIDK 130
           + L WT+  +      +V++HV  T+         K+P   + E  V + +R  +++ ++
Sbjct: 40  RALRWTIENFLPKIDRLVLVHVMPTVTTIPSPSGSKIPIEELDESVVSMYKRDLRKEFEQ 99

Query: 131 LLSKYVDFCGKVKAEILKVEKSDEPVNKLLVDLISELGITNLVIGFTFMKSSSWKSKNAI 190
           +   +   C   K E L +E  D    K L+  +S+  +  LVIG     S+    K   
Sbjct: 100 VFVPFKRICKSNKVETLLLEHHDPA--KALLKYMSDTDVECLVIG--SCSSNFLTRKKGQ 159

Query: 191 SGSFYIYRNKAHFCELFVIWGGKQVFLRDERIMEDDKGVRIAKISSKPSLKGWLGRIFVE 250
                +       CE++V+          +RI+        A  SS   +          
Sbjct: 160 EMPLTVLGEAPETCEIYVVC--------KDRILTKSTNQFTADSSSSFRIPEGAEAYTES 219

Query: 251 DPNYYSERNLSLSSASPSSSNSSSSRNYWDSNVHELENYYEELLS----LNLQEEEDCEQ 310
                S++    +S+  SS      R     + H     Y +  S    + L ++E C  
Sbjct: 220 FSRTRSDKTGLSASSITSSGRMRIGRPGSLPHSHPTSRVYSDAQSSSTDIVLVDDEHCRS 279

Query: 311 DQDGGLESSGSTQFNVLDH---PDSNMNAEERIEHLRTKIEDARKTVQLMKDEAKGSAER 370
                  S+   Q +   H   P S + AE  +E LR +++    T+ + K   +    +
Sbjct: 280 ILRHSTVSTSKIQMDPRPHLKTPKSGVRAE--VEQLRKEVQ---TTLSMYKQACEELVHK 339

Query: 371 QAKAEWAINLCSQRTEELEAQIKEEVTVREELKKELDSTKEYILEIVGDIEESKIRLSSL 430
           Q + +   + C + TE +   +++E    E  +K     KE  L+ V ++EE+K      
Sbjct: 340 QTQVQSLSSECIKETERVITALEKE----EMRRKAAAEEKEKHLKAVKEVEEAK------ 399

Query: 431 LELQAELSSKLLISTVGKSRIEAQLEKTAKTREGMVRE--IEQLRRQRDILHRRIEFCKE 490
                        S + K   E QL +    ++ + ++  IEQL   RD  +R+      
Sbjct: 400 -------------SMLAKEFCERQLAELDALKQSIEKQKVIEQL-FLRDGRYRK------ 459

Query: 491 KDAIGMAERSTEVSCSTRVYTAEEIRLATDSFSEQMRLS----SRMYRGRINHTSVAIQM 550
                              YT EEI  ATD+FS +  +      ++Y+  ++HT VA+++
Sbjct: 460 -------------------YTKEEIAAATDNFSSRKIIGEGGYGKVYKCSLDHTPVALKV 519

Query: 551 IDSGNRLSEEDFQSKVELLSNIRHPHLIAIMGFCSELKCIVFDYMHGGSLSDRLLPSNKR 610
           +   +   +E+F  ++ +LS +RHPH++ ++G C E  C+V++YM  GSL   + P   +
Sbjct: 520 LKPDSVEKKEEFLKEISVLSQLRHPHVVLLLGACPENGCLVYEYMENGSLDCHISPKKGK 579

Query: 611 SRKICRSLMWHDRIRIASEVCSGLSFLHQAQPQPISHGSLTLSKILLDQNLVAKITGSGL 670
                 SL W  R RI  E   GL+FLH ++P+PI H  L    ILLD+N V+KI   GL
Sbjct: 580 P-----SLSWFIRFRIIYETACGLAFLHNSKPEPIVHRDLKPGNILLDRNFVSKIGDVGL 639

Query: 671 VEL--DEA--------------------------GGI--ELDIRAFGALLLHLVTGRNWE 730
            +L  DEA                          G I  + D+ AFG ++L L+T R+  
Sbjct: 640 AKLMSDEAPDSVTVYRNSIIAGTLYYMDPEYQRTGTIRPKSDLYAFGIIILQLLTARHPN 699

Query: 731 GLIEKGLTMGKVGLVQ-ILDDKAGKWPLDLVDALVDLALRCVTPNGSNSEVNLRTAMEEI 790
           GL+       K G  + +LD     WP+     L  +A+RC       S++  R   +  
Sbjct: 700 GLLFCVEDAVKRGCFEDMLDGSVKDWPIAEAKELARIAIRC-------SQLKCRDRPDLS 759

Query: 791 DEIKRRADDLVENGKHAGGTEGADAANEDVEDVPRLFICPILQEVMKNPHVAADGFSYEL 842
            ++      ++E+      TE A+A        P  + CPIL+E+M++P +AADGF+YE 
Sbjct: 760 TQVLPALKRILESANSRLKTEQANAR------APTHYYCPILKEIMEDPQIAADGFTYER 778