Spg012114 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg012114 vs. NCBI nr
Match: XP_038883935.1 (uncharacterized protein LOC120074765 [Benincasa hispida])

HSP 1 Score: 880.9 bits (2275), Expect = 5.0e-252
Identity = 438/499 (87.78%), Postives = 462/499 (92.59%), Query Frame = 0

Query: 1   MVVATMPWLSGSIKGTTGHTLQFFFHDKTPPPTLRILAFDTAKTMAALISLYRSLSDDEI 60
           MVVATMPWLSGSIKGTT HTLQFFF DK+ PPTLRIL FDTAKTMAALISLYRSLSDDEI
Sbjct: 1   MVVATMPWLSGSIKGTTNHTLQFFFQDKSTPPTLRILCFDTAKTMAALISLYRSLSDDEI 60

Query: 61  NNLQKHALRSQGVLYLNSRNEDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120
           + LQ H LRS+GV YLNSR+E+FLL LACSERLEELNNAASSVSRLSRKCADLGLTRFDL
Sbjct: 61  SRLQNHILRSKGVAYLNSRDEEFLLNLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120

Query: 121 VFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWR 180
           +FSDMKLGIFH  KSDSGFKNV KLI RMEKLVFLTAELHSAME L+EMEASEKK+QKWR
Sbjct: 121 LFSDMKLGIFHSVKSDSGFKNVAKLIARMEKLVFLTAELHSAMESLIEMEASEKKLQKWR 180

Query: 181 AVTPKQCLPVNFELFDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTV 240
            ++PKQ  PVNFELFDKKLA+Q+KDVKHFKEISLWNQSFDYAVGLMTRL+C IYARI TV
Sbjct: 181 TMSPKQFPPVNFELFDKKLASQKKDVKHFKEISLWNQSFDYAVGLMTRLVCFIYARIVTV 240

Query: 241 FGSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKCSDNDHRLVTQSGPIPKKGKKELI 300
           FG   PDQ+C +  NPQIRILRDRVWRWNFYGG+RKC DN++RLVTQSGPIPKKGKKEL+
Sbjct: 241 FGPLAPDQACPMYHNPQIRILRDRVWRWNFYGGNRKCGDNEYRLVTQSGPIPKKGKKELV 300

Query: 301 RFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLHA 360
           RFPSGIRAKDDIGIGYG FNSST ANNRVYTSAPPTTVGGSGLSMNYANVILFAERCL A
Sbjct: 301 RFPSGIRAKDDIGIGYGEFNSSTGANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLLA 360

Query: 361 PATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREAV 420
           PATIGEEARGELYEMLP RIK KVRAKL+RNNWVKRG GEEEMG G DG+SLAAGWREAV
Sbjct: 361 PATIGEEARGELYEMLPARIKEKVRAKLRRNNWVKRG-GEEEMGYGRDGNSLAAGWREAV 420

Query: 421 EEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGLS 480
           EEMMGWLGPLAHDTVRWQSERNMEKQRFDT+PTALLMQTLHYSDLEKTEAAIVEVLVGLS
Sbjct: 421 EEMMGWLGPLAHDTVRWQSERNMEKQRFDTNPTALLMQTLHYSDLEKTEAAIVEVLVGLS 480

Query: 481 CIYRYENRRPQGRRSSDHV 500
           CIYRYENRRP   R+SDH+
Sbjct: 481 CIYRYENRRPPCCRTSDHM 498

BLAST of Spg012114 vs. NCBI nr
Match: KAA0032674.1 (Avr9/Cf-9 rapidly elicited protein [Cucumis melo var. makuwa] >TYK23557.1 Avr9/Cf-9 rapidly elicited protein [Cucumis melo var. makuwa])

HSP 1 Score: 872.5 bits (2253), Expect = 1.8e-249
Identity = 434/500 (86.80%), Postives = 458/500 (91.60%), Query Frame = 0

Query: 1   MVVATMPWLSGSIKGTTGHTLQFFFHDKTPPPTLRILAFDTAKTMAALISLYRSLSDDEI 60
           MVVATMPWLS SIKGTT  TLQFFF DK+ PPTLRIL FDTAKTMAALISLYRSLSDDEI
Sbjct: 1   MVVATMPWLSSSIKGTTNQTLQFFFQDKSTPPTLRILCFDTAKTMAALISLYRSLSDDEI 60

Query: 61  NNLQKHALRSQGVLYLNSRNEDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120
           + LQ   LRS+GV YLNSR+E+FLL LACSERLEELNNAASSVSRLSRKCADLGLTRFDL
Sbjct: 61  SRLQNDTLRSKGVSYLNSRDEEFLLNLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120

Query: 121 VFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWR 180
           +FSDMKLGIFH  KSD GFKNV KLI RMEKLV LTAELHSAME L EMEASEKK+QKW+
Sbjct: 121 LFSDMKLGIFHSAKSDPGFKNVAKLISRMEKLVLLTAELHSAMEALTEMEASEKKLQKWK 180

Query: 181 AVTPKQCLPVNFELFDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTV 240
            + PKQ  PVNFELFDKKL++QRKDVKHFKEISLWNQSFDYAVG+MTRL+C IYARI T+
Sbjct: 181 QLGPKQFPPVNFELFDKKLSSQRKDVKHFKEISLWNQSFDYAVGMMTRLVCFIYARIVTI 240

Query: 241 FGSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKC-SDNDHRLVTQSGPIPKKGKKEL 300
           FG  VPDQ+CSL  NPQIRILRDRVWRWNFYGG+RKC SDN++RLVTQSGPIPKKGKKEL
Sbjct: 241 FGPLVPDQACSLYHNPQIRILRDRVWRWNFYGGNRKCSSDNEYRLVTQSGPIPKKGKKEL 300

Query: 301 IRFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLH 360
           +RFPSGIRAKDDIGIG G FNSST ANNRVYTSAPP TVGGSGLS+NYANVILFAERCLH
Sbjct: 301 VRFPSGIRAKDDIGIGCGEFNSSTPANNRVYTSAPPMTVGGSGLSINYANVILFAERCLH 360

Query: 361 APATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREA 420
           APATIGEEARGELYEMLP  IK KVRAKL+RNNWVKRG G EEMGSGGDGHSLAAGWREA
Sbjct: 361 APATIGEEARGELYEMLPAGIKEKVRAKLRRNNWVKRGEGAEEMGSGGDGHSLAAGWREA 420

Query: 421 VEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGL 480
           VEEM+GWLGPLAHDTVRWQSERNMEKQRFDT+PTALLMQTLHYSDLEKTEAAIVEVLVGL
Sbjct: 421 VEEMLGWLGPLAHDTVRWQSERNMEKQRFDTNPTALLMQTLHYSDLEKTEAAIVEVLVGL 480

Query: 481 SCIYRYENRRPQGRRSSDHV 500
           SCIYRYEN RPQ RRSSDH+
Sbjct: 481 SCIYRYENPRPQCRRSSDHM 500

BLAST of Spg012114 vs. NCBI nr
Match: XP_004142198.1 (uncharacterized protein LOC101204955 [Cucumis sativus] >KGN54272.1 hypothetical protein Csa_018148 [Cucumis sativus])

HSP 1 Score: 868.2 bits (2242), Expect = 3.4e-248
Identity = 431/500 (86.20%), Postives = 459/500 (91.80%), Query Frame = 0

Query: 1   MVVATMPWLSGSIKGTTGHTLQFFFHDKTPPPTLRILAFDTAKTMAALISLYRSLSDDEI 60
           MVVATMPWLS SIKGTT HTLQFFFHDK+ PPTLRIL FDTAKTMAALISLYRSLSDDEI
Sbjct: 1   MVVATMPWLSSSIKGTTNHTLQFFFHDKSTPPTLRILCFDTAKTMAALISLYRSLSDDEI 60

Query: 61  NNLQKHALRSQGVLYLNSRNEDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120
           + LQ   LRS+GV YLNSR+E+FLL LACSERLEE+NNAASSVSRLSRKCADLGLTRFDL
Sbjct: 61  SRLQNDTLRSKGVSYLNSRDEEFLLNLACSERLEEMNNAASSVSRLSRKCADLGLTRFDL 120

Query: 121 VFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWR 180
           +FSDMKLGIFH  KSDSG KNV KLI RMEKLVFLT+ELHSAME L EME SEKK+QKW+
Sbjct: 121 LFSDMKLGIFHSAKSDSGCKNVAKLIARMEKLVFLTSELHSAMEGLTEMEVSEKKLQKWK 180

Query: 181 AVTPKQCLPVNFELFDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTV 240
           A++PKQ  PVNFELFDKKLA+QRKDVKHFKEISLWNQSFDYAVG+MTRL+C+IY RI TV
Sbjct: 181 ALSPKQFPPVNFELFDKKLASQRKDVKHFKEISLWNQSFDYAVGMMTRLVCLIYTRIVTV 240

Query: 241 FGSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKC-SDNDHRLVTQSGPIPKKGKKEL 300
           FG  VPD +CSL  NPQIRILRDRVWRWNFYG +RKC SDN++RLVTQSGPIPKKGKKEL
Sbjct: 241 FGPLVPDLACSLYHNPQIRILRDRVWRWNFYGVNRKCSSDNEYRLVTQSGPIPKKGKKEL 300

Query: 301 IRFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLH 360
           +RFPSGIRAKDD+GIGYG FNSST  NNRVYTSAPPTTVGGSGLS+NYANVILFAERCLH
Sbjct: 301 VRFPSGIRAKDDMGIGYGEFNSSTPENNRVYTSAPPTTVGGSGLSINYANVILFAERCLH 360

Query: 361 APATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREA 420
           APATIG+EARGELYEMLP  IK KVRAKL+RNNWVKRG G EE+GSGGDGHSLAAGWREA
Sbjct: 361 APATIGDEARGELYEMLPAGIKEKVRAKLRRNNWVKRGEGAEELGSGGDGHSLAAGWREA 420

Query: 421 VEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGL 480
           VEEMMGWLGPLAHDTVRWQSERNMEKQRFD +PTALLMQTLHYSDLEKTEAAIVEVLVGL
Sbjct: 421 VEEMMGWLGPLAHDTVRWQSERNMEKQRFDMNPTALLMQTLHYSDLEKTEAAIVEVLVGL 480

Query: 481 SCIYRYENRRPQGRRSSDHV 500
           SCIYRYEN R Q RRSSDH+
Sbjct: 481 SCIYRYENPRLQCRRSSDHM 500

BLAST of Spg012114 vs. NCBI nr
Match: XP_008449997.1 (PREDICTED: uncharacterized protein LOC103491711, partial [Cucumis melo])

HSP 1 Score: 825.9 bits (2132), Expect = 1.9e-235
Identity = 411/473 (86.89%), Postives = 435/473 (91.97%), Query Frame = 0

Query: 28  KTPPPTLRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRNEDFLLKL 87
           K+ PPTLRIL FDTAKTMAALISLYRSLSDDEI+ LQ   LRS+GV YLNSR+E+FLL L
Sbjct: 1   KSTPPTLRILCFDTAKTMAALISLYRSLSDDEISRLQNDTLRSKGVSYLNSRDEEFLLNL 60

Query: 88  ACSERLEELNNAASSVSRLSRKCADLGLTRFDLVFSDMKLGIFHWGKSDSGFKNVGKLIG 147
           ACSERLEELNNAASSVSRLSRKCADLGLTRFDL+FSDMKLGIFH  KSD GFKNV KLI 
Sbjct: 61  ACSERLEELNNAASSVSRLSRKCADLGLTRFDLLFSDMKLGIFHSAKSDPGFKNVAKLIS 120

Query: 148 RMEKLVFLTAELHSAMEVLLEMEASEKKMQKWRAVTPKQCLPVNFELFDKKLATQRKDVK 207
           RMEKLV LTAELHSAME L EMEASEKK+QKW+ + PKQ  PVNFELFDKKL++QRKDVK
Sbjct: 121 RMEKLVLLTAELHSAMEALTEMEASEKKLQKWKQLGPKQFPPVNFELFDKKLSSQRKDVK 180

Query: 208 HFKEISLWNQSFDYAVGLMTRLICVIYARISTVFGSFVPDQSCSLDQNPQIRILRDRVWR 267
           HFKEISLWNQSFDYAVG+MTRL+C IYARI T+FG  VPDQ+CSL  NPQIRILRDRVWR
Sbjct: 181 HFKEISLWNQSFDYAVGMMTRLVCFIYARIVTIFGPLVPDQACSLYHNPQIRILRDRVWR 240

Query: 268 WNFYGGHRKC-SDNDHRLVTQSGPIPKKGKKELIRFPSGIRAKDDIGIGYGVFNSSTAAN 327
           WNFYGG+RKC SDN++RLVTQSGPIPKKGKKEL+RFPSGIRAKDDIGIG G FNSST AN
Sbjct: 241 WNFYGGNRKCSSDNEYRLVTQSGPIPKKGKKELVRFPSGIRAKDDIGIGCGEFNSSTPAN 300

Query: 328 NRVYTSAPPTTVGGSGLSMNYANVILFAERCLHAPATIGEEARGELYEMLPTRIKAKVRA 387
           NRVYTSAPP TVGGSGLS+NYANVILFAERCLHAPATIGEEARGELYEMLP  IK KVRA
Sbjct: 301 NRVYTSAPPMTVGGSGLSINYANVILFAERCLHAPATIGEEARGELYEMLPAGIKEKVRA 360

Query: 388 KLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREAVEEMMGWLGPLAHDTVRWQSERNMEKQ 447
           KL+RNNWVKRG G EEMGSGGDGHSLAAGWREAVEEM+GWLGPLAHDTVRWQSERNMEKQ
Sbjct: 361 KLRRNNWVKRGEGAEEMGSGGDGHSLAAGWREAVEEMLGWLGPLAHDTVRWQSERNMEKQ 420

Query: 448 RFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGLSCIYRYENRRPQGRRSSDHV 500
           RFDT+PTALLMQTLHYSDLEKTEAAIVEVLVGLSCIYRYEN RPQ RRSSDH+
Sbjct: 421 RFDTNPTALLMQTLHYSDLEKTEAAIVEVLVGLSCIYRYENPRPQCRRSSDHM 473

BLAST of Spg012114 vs. NCBI nr
Match: XP_022931864.1 (uncharacterized protein LOC111438146 [Cucurbita moschata])

HSP 1 Score: 816.6 bits (2108), Expect = 1.2e-232
Identity = 404/499 (80.96%), Postives = 443/499 (88.78%), Query Frame = 0

Query: 1   MVVATMPWLSGSIKGTTGHTLQFFFHDKTPPPTLRILAFDTAKTMAALISLYRSLSDDEI 60
           MVVATMPWL+GS+KG + HTL FFF DKTPPPTLRILAFDTAKTMAALISLYRSL+D EI
Sbjct: 1   MVVATMPWLTGSLKGASNHTLHFFFQDKTPPPTLRILAFDTAKTMAALISLYRSLTDKEI 60

Query: 61  NNLQKHALRSQGVLYLNSRNEDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120
           + L+ H + S+GV+YLNS +E+FLL LACSERLEELNNAASSVSRLSRKCADLGLTRFDL
Sbjct: 61  SKLRNHVIPSKGVVYLNSMDEEFLLNLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120

Query: 121 VFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWR 180
           VFSDMKLGIF+WGKSDSGFKNV KLIG+MEKLVF TAELHSAMEVL+EMEASEKKMQK +
Sbjct: 121 VFSDMKLGIFNWGKSDSGFKNVAKLIGKMEKLVFATAELHSAMEVLVEMEASEKKMQKRK 180

Query: 181 AVTPKQCLPVNFELFDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTV 240
            +TPKQ +P+ F++FDKKLA+QRKDVKHFKEISLWNQSFDYAVGLMTRL+CVIYARI  V
Sbjct: 181 EITPKQGVPLKFDMFDKKLASQRKDVKHFKEISLWNQSFDYAVGLMTRLVCVIYARIFAV 240

Query: 241 FGSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKC--SDNDHRLVTQSGPIPKKGKKE 300
           F  FV DQ CSLDQNPQI+ L +RVWRWNF+G HRK    D++H+LVTQSGPIPK GKKE
Sbjct: 241 FRPFVSDQVCSLDQNPQIQNLSNRVWRWNFHGEHRKVGGGDDEHKLVTQSGPIPKNGKKE 300

Query: 301 LIRFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCL 360
           LIRFPSGIR K++  I YG F+SS A NNRVYTSAPPTTVGGSGLS+NYANVILFAERCL
Sbjct: 301 LIRFPSGIREKNNPRIEYGEFDSSMATNNRVYTSAPPTTVGGSGLSLNYANVILFAERCL 360

Query: 361 HAPATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWRE 420
           +   TIG++ARGELY+MLP RIK KVRAKL+RNNW KRG G+EEM S  DGHSLA GWRE
Sbjct: 361 NEATTIGDDARGELYQMLPARIKEKVRAKLRRNNWGKRGGGKEEMRSADDGHSLAVGWRE 420

Query: 421 AVEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVG 480
           A+EEMMGWLGPLAHDTVRWQSERNMEKQRFDT  T LLMQTLHYSDLEKTEAAIVEVLVG
Sbjct: 421 AMEEMMGWLGPLAHDTVRWQSERNMEKQRFDTGSTVLLMQTLHYSDLEKTEAAIVEVLVG 480

Query: 481 LSCIYRYENRRPQGRRSSD 498
           L CIYRYENRRPQ  RS D
Sbjct: 481 LCCIYRYENRRPQDHRSCD 499

BLAST of Spg012114 vs. ExPASy Swiss-Prot
Match: Q9XID5 (Protein PSK SIMULATOR 1 OS=Arabidopsis thaliana OX=3702 GN=PSI1 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.3e-18
Identity = 101/458 (22.05%), Postives = 189/458 (41.27%), Query Frame = 0

Query: 34  LRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRNEDFLLKLACSERL 93
           + IL+F+ A T+    +L  SLS D I +L++  L S+GV  L S++ D LL++A +++ 
Sbjct: 155 ISILSFEVANTIVKGANLMHSLSKDSITHLKEVVLPSEGVQNLISKDMDELLRIAAADKR 214

Query: 94  EELNNAASSVSRLSRKCADLGLTRFDLVFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLV 153
           EEL   +  V R   +C D      D  F   +LG   +       +    ++ +M   V
Sbjct: 215 EELRIFSGEVVRFGNRCKDPQYHNLDRFFD--RLG-SEFTPQKHLKQEAETIMHQMMSFV 274

Query: 154 FLTAELHSAMEVL--LEMEASEKKMQKWRAVTPKQCLPVNFELFDKKLATQRKDVKHFKE 213
             TA+L+  +  L   E +   K  ++    T ++ +     +   +L +Q+K V++ K+
Sbjct: 275 HFTADLYHELHALDRFEQDYQRKIQEEENPSTAQRGVGDTLAILRTELKSQKKHVRNLKK 334

Query: 214 ISLWNQSFDYAVGLMTRLICVIYARISTVFGSFVPDQSCSLDQNPQIRILRDRVWRWNFY 273
            SLW++  +  +  +  ++  ++  I   FG   PD+  +   +P I             
Sbjct: 335 KSLWSRILEEVMEKLVDVVHFLHLEIHEAFGGADPDKPAN---DPPI------------- 394

Query: 274 GGHRKCSDNDHRLVTQSGPIPKKGKKELIRFPSGIRAKDDIGIGYGVFNSSTAANNRVYT 333
             H+K                                                       
Sbjct: 395 -NHKK------------------------------------------------------- 454

Query: 334 SAPPTTVGGSGLSMNYANVILFAERCLHAPATIGEEARGELYEMLPTRIKAKVRAKLKRN 393
                 +G +GL+++YAN+I   +  +   +T+    R  LY+ LP  IK+ +R+++ ++
Sbjct: 455 ------LGSAGLALHYANIITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRI-QS 514

Query: 394 NWVKRGAGEEEMGSGGDGHSLAAGWREAVEEMMGWLGPLAHDTVR----------WQSER 453
             VK      ++             +  +E+ + WL P+A +T +          W S  
Sbjct: 515 FQVKEELTVPQI-------------KAEMEKTLQWLVPVATNTTKAHHGFGWVGEWASSG 516

Query: 454 NMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGL 480
           +   QR     T L + TLH++D EKTEA I++++V L
Sbjct: 575 SEANQR-PAGQTILRIDTLHHADKEKTEAYILDLVVWL 516

BLAST of Spg012114 vs. ExPASy Swiss-Prot
Match: P0DO24 (Protein PSK SIMULATOR 3 OS=Arabidopsis thaliana OX=3702 GN=PSI3 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.4e-15
Identity = 101/469 (21.54%), Postives = 174/469 (37.10%), Query Frame = 0

Query: 34  LRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRNEDFLLKLACSERL 93
           L ILAF+ A T+    +L  SLS   I +L+   L S+GV  L S + D LL+L  +++ 
Sbjct: 149 LGILAFEVANTIVKSSNLIESLSKRNIEHLKGTILYSEGVQNLVSNDFDELLRLVAADKR 208

Query: 94  EELNNAASSVSRLSRKCADLGLTRFDLVFSDMKLGIFHWGKSDSGFKNVGK--------- 153
           +EL   +  V R   +  D                 F W      F  + K         
Sbjct: 209 QELQVFSGEVVRFGNRSKD-----------------FQWHNLQRYFDRISKELTPQRQLK 268

Query: 154 -----LIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWRAVTPKQCLPVNFELFDKKL 213
                ++ ++  LV  TAEL+  ++VL  +E   ++ ++    +          +   +L
Sbjct: 269 EDAVLVVDQLMVLVQYTAELYQELQVLYRLEKDYEQKRREEENSANSSKGDGLAILKTEL 328

Query: 214 ATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTVFGSFVPDQSCSLDQNPQIR 273
             QRK VK  K+ SLW++ F+  +  +  ++  +   I  +FG          D  P   
Sbjct: 329 KAQRKVVKSLKKKSLWSRGFEEVMEKLVDIVHFLLLEIHNIFG--------GADDQPS-- 388

Query: 274 ILRDRVWRWNFYGGHRKCSDNDHRLVTQSGPIPKKGKKELIRFPSGIRAKDDIGIGYGVF 333
                                            KKG  E  +                  
Sbjct: 389 ---------------------------------KKGAAEYDK------------------ 448

Query: 334 NSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLHAPATIGEEARGELYEMLPTR 393
                             +G +GL+++YAN+I+  +  +   ++I   AR  LY+ LP  
Sbjct: 449 -----------------RLGPAGLALHYANIIVQIDTLVARASSITSNARDSLYQSLPPG 508

Query: 394 IKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREAVEEMMGWLGPLAHDTVR--- 453
           IK  +R+K+K  N               D        ++ +E  + WL P+A +T +   
Sbjct: 509 IKLALRSKIKSFN--------------VDKELSVTQIKDEMERTLHWLVPVAGNTTKAHH 508

Query: 454 ---WQSERNMEKQRFDTSPTA---LLMQTLHYSDLEKTEAAIVEVLVGL 480
              W  E       F + P+    L ++TL+++  EKTE  I+  ++ L
Sbjct: 569 GFGWVGEWANTGTDFTSKPSGGDILRIETLYHASKEKTEIYILGQIIWL 508

BLAST of Spg012114 vs. ExPASy TrEMBL
Match: A0A5D3DIU8 (Avr9/Cf-9 rapidly elicited protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00650 PE=4 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 8.7e-250
Identity = 434/500 (86.80%), Postives = 458/500 (91.60%), Query Frame = 0

Query: 1   MVVATMPWLSGSIKGTTGHTLQFFFHDKTPPPTLRILAFDTAKTMAALISLYRSLSDDEI 60
           MVVATMPWLS SIKGTT  TLQFFF DK+ PPTLRIL FDTAKTMAALISLYRSLSDDEI
Sbjct: 1   MVVATMPWLSSSIKGTTNQTLQFFFQDKSTPPTLRILCFDTAKTMAALISLYRSLSDDEI 60

Query: 61  NNLQKHALRSQGVLYLNSRNEDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120
           + LQ   LRS+GV YLNSR+E+FLL LACSERLEELNNAASSVSRLSRKCADLGLTRFDL
Sbjct: 61  SRLQNDTLRSKGVSYLNSRDEEFLLNLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120

Query: 121 VFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWR 180
           +FSDMKLGIFH  KSD GFKNV KLI RMEKLV LTAELHSAME L EMEASEKK+QKW+
Sbjct: 121 LFSDMKLGIFHSAKSDPGFKNVAKLISRMEKLVLLTAELHSAMEALTEMEASEKKLQKWK 180

Query: 181 AVTPKQCLPVNFELFDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTV 240
            + PKQ  PVNFELFDKKL++QRKDVKHFKEISLWNQSFDYAVG+MTRL+C IYARI T+
Sbjct: 181 QLGPKQFPPVNFELFDKKLSSQRKDVKHFKEISLWNQSFDYAVGMMTRLVCFIYARIVTI 240

Query: 241 FGSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKC-SDNDHRLVTQSGPIPKKGKKEL 300
           FG  VPDQ+CSL  NPQIRILRDRVWRWNFYGG+RKC SDN++RLVTQSGPIPKKGKKEL
Sbjct: 241 FGPLVPDQACSLYHNPQIRILRDRVWRWNFYGGNRKCSSDNEYRLVTQSGPIPKKGKKEL 300

Query: 301 IRFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLH 360
           +RFPSGIRAKDDIGIG G FNSST ANNRVYTSAPP TVGGSGLS+NYANVILFAERCLH
Sbjct: 301 VRFPSGIRAKDDIGIGCGEFNSSTPANNRVYTSAPPMTVGGSGLSINYANVILFAERCLH 360

Query: 361 APATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREA 420
           APATIGEEARGELYEMLP  IK KVRAKL+RNNWVKRG G EEMGSGGDGHSLAAGWREA
Sbjct: 361 APATIGEEARGELYEMLPAGIKEKVRAKLRRNNWVKRGEGAEEMGSGGDGHSLAAGWREA 420

Query: 421 VEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGL 480
           VEEM+GWLGPLAHDTVRWQSERNMEKQRFDT+PTALLMQTLHYSDLEKTEAAIVEVLVGL
Sbjct: 421 VEEMLGWLGPLAHDTVRWQSERNMEKQRFDTNPTALLMQTLHYSDLEKTEAAIVEVLVGL 480

Query: 481 SCIYRYENRRPQGRRSSDHV 500
           SCIYRYEN RPQ RRSSDH+
Sbjct: 481 SCIYRYENPRPQCRRSSDHM 500

BLAST of Spg012114 vs. ExPASy TrEMBL
Match: A0A0A0L0Z9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G296260 PE=4 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.6e-248
Identity = 431/500 (86.20%), Postives = 459/500 (91.80%), Query Frame = 0

Query: 1   MVVATMPWLSGSIKGTTGHTLQFFFHDKTPPPTLRILAFDTAKTMAALISLYRSLSDDEI 60
           MVVATMPWLS SIKGTT HTLQFFFHDK+ PPTLRIL FDTAKTMAALISLYRSLSDDEI
Sbjct: 1   MVVATMPWLSSSIKGTTNHTLQFFFHDKSTPPTLRILCFDTAKTMAALISLYRSLSDDEI 60

Query: 61  NNLQKHALRSQGVLYLNSRNEDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120
           + LQ   LRS+GV YLNSR+E+FLL LACSERLEE+NNAASSVSRLSRKCADLGLTRFDL
Sbjct: 61  SRLQNDTLRSKGVSYLNSRDEEFLLNLACSERLEEMNNAASSVSRLSRKCADLGLTRFDL 120

Query: 121 VFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWR 180
           +FSDMKLGIFH  KSDSG KNV KLI RMEKLVFLT+ELHSAME L EME SEKK+QKW+
Sbjct: 121 LFSDMKLGIFHSAKSDSGCKNVAKLIARMEKLVFLTSELHSAMEGLTEMEVSEKKLQKWK 180

Query: 181 AVTPKQCLPVNFELFDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTV 240
           A++PKQ  PVNFELFDKKLA+QRKDVKHFKEISLWNQSFDYAVG+MTRL+C+IY RI TV
Sbjct: 181 ALSPKQFPPVNFELFDKKLASQRKDVKHFKEISLWNQSFDYAVGMMTRLVCLIYTRIVTV 240

Query: 241 FGSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKC-SDNDHRLVTQSGPIPKKGKKEL 300
           FG  VPD +CSL  NPQIRILRDRVWRWNFYG +RKC SDN++RLVTQSGPIPKKGKKEL
Sbjct: 241 FGPLVPDLACSLYHNPQIRILRDRVWRWNFYGVNRKCSSDNEYRLVTQSGPIPKKGKKEL 300

Query: 301 IRFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLH 360
           +RFPSGIRAKDD+GIGYG FNSST  NNRVYTSAPPTTVGGSGLS+NYANVILFAERCLH
Sbjct: 301 VRFPSGIRAKDDMGIGYGEFNSSTPENNRVYTSAPPTTVGGSGLSINYANVILFAERCLH 360

Query: 361 APATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREA 420
           APATIG+EARGELYEMLP  IK KVRAKL+RNNWVKRG G EE+GSGGDGHSLAAGWREA
Sbjct: 361 APATIGDEARGELYEMLPAGIKEKVRAKLRRNNWVKRGEGAEELGSGGDGHSLAAGWREA 420

Query: 421 VEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGL 480
           VEEMMGWLGPLAHDTVRWQSERNMEKQRFD +PTALLMQTLHYSDLEKTEAAIVEVLVGL
Sbjct: 421 VEEMMGWLGPLAHDTVRWQSERNMEKQRFDMNPTALLMQTLHYSDLEKTEAAIVEVLVGL 480

Query: 481 SCIYRYENRRPQGRRSSDHV 500
           SCIYRYEN R Q RRSSDH+
Sbjct: 481 SCIYRYENPRLQCRRSSDHM 500

BLAST of Spg012114 vs. ExPASy TrEMBL
Match: A0A1S3BMP0 (uncharacterized protein LOC103491711 OS=Cucumis melo OX=3656 GN=LOC103491711 PE=4 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 9.3e-236
Identity = 411/473 (86.89%), Postives = 435/473 (91.97%), Query Frame = 0

Query: 28  KTPPPTLRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRNEDFLLKL 87
           K+ PPTLRIL FDTAKTMAALISLYRSLSDDEI+ LQ   LRS+GV YLNSR+E+FLL L
Sbjct: 1   KSTPPTLRILCFDTAKTMAALISLYRSLSDDEISRLQNDTLRSKGVSYLNSRDEEFLLNL 60

Query: 88  ACSERLEELNNAASSVSRLSRKCADLGLTRFDLVFSDMKLGIFHWGKSDSGFKNVGKLIG 147
           ACSERLEELNNAASSVSRLSRKCADLGLTRFDL+FSDMKLGIFH  KSD GFKNV KLI 
Sbjct: 61  ACSERLEELNNAASSVSRLSRKCADLGLTRFDLLFSDMKLGIFHSAKSDPGFKNVAKLIS 120

Query: 148 RMEKLVFLTAELHSAMEVLLEMEASEKKMQKWRAVTPKQCLPVNFELFDKKLATQRKDVK 207
           RMEKLV LTAELHSAME L EMEASEKK+QKW+ + PKQ  PVNFELFDKKL++QRKDVK
Sbjct: 121 RMEKLVLLTAELHSAMEALTEMEASEKKLQKWKQLGPKQFPPVNFELFDKKLSSQRKDVK 180

Query: 208 HFKEISLWNQSFDYAVGLMTRLICVIYARISTVFGSFVPDQSCSLDQNPQIRILRDRVWR 267
           HFKEISLWNQSFDYAVG+MTRL+C IYARI T+FG  VPDQ+CSL  NPQIRILRDRVWR
Sbjct: 181 HFKEISLWNQSFDYAVGMMTRLVCFIYARIVTIFGPLVPDQACSLYHNPQIRILRDRVWR 240

Query: 268 WNFYGGHRKC-SDNDHRLVTQSGPIPKKGKKELIRFPSGIRAKDDIGIGYGVFNSSTAAN 327
           WNFYGG+RKC SDN++RLVTQSGPIPKKGKKEL+RFPSGIRAKDDIGIG G FNSST AN
Sbjct: 241 WNFYGGNRKCSSDNEYRLVTQSGPIPKKGKKELVRFPSGIRAKDDIGIGCGEFNSSTPAN 300

Query: 328 NRVYTSAPPTTVGGSGLSMNYANVILFAERCLHAPATIGEEARGELYEMLPTRIKAKVRA 387
           NRVYTSAPP TVGGSGLS+NYANVILFAERCLHAPATIGEEARGELYEMLP  IK KVRA
Sbjct: 301 NRVYTSAPPMTVGGSGLSINYANVILFAERCLHAPATIGEEARGELYEMLPAGIKEKVRA 360

Query: 388 KLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREAVEEMMGWLGPLAHDTVRWQSERNMEKQ 447
           KL+RNNWVKRG G EEMGSGGDGHSLAAGWREAVEEM+GWLGPLAHDTVRWQSERNMEKQ
Sbjct: 361 KLRRNNWVKRGEGAEEMGSGGDGHSLAAGWREAVEEMLGWLGPLAHDTVRWQSERNMEKQ 420

Query: 448 RFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGLSCIYRYENRRPQGRRSSDHV 500
           RFDT+PTALLMQTLHYSDLEKTEAAIVEVLVGLSCIYRYEN RPQ RRSSDH+
Sbjct: 421 RFDTNPTALLMQTLHYSDLEKTEAAIVEVLVGLSCIYRYENPRPQCRRSSDHM 473

BLAST of Spg012114 vs. ExPASy TrEMBL
Match: A0A6J1EVF1 (uncharacterized protein LOC111438146 OS=Cucurbita moschata OX=3662 GN=LOC111438146 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.6e-233
Identity = 404/499 (80.96%), Postives = 443/499 (88.78%), Query Frame = 0

Query: 1   MVVATMPWLSGSIKGTTGHTLQFFFHDKTPPPTLRILAFDTAKTMAALISLYRSLSDDEI 60
           MVVATMPWL+GS+KG + HTL FFF DKTPPPTLRILAFDTAKTMAALISLYRSL+D EI
Sbjct: 1   MVVATMPWLTGSLKGASNHTLHFFFQDKTPPPTLRILAFDTAKTMAALISLYRSLTDKEI 60

Query: 61  NNLQKHALRSQGVLYLNSRNEDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120
           + L+ H + S+GV+YLNS +E+FLL LACSERLEELNNAASSVSRLSRKCADLGLTRFDL
Sbjct: 61  SKLRNHVIPSKGVVYLNSMDEEFLLNLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120

Query: 121 VFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWR 180
           VFSDMKLGIF+WGKSDSGFKNV KLIG+MEKLVF TAELHSAMEVL+EMEASEKKMQK +
Sbjct: 121 VFSDMKLGIFNWGKSDSGFKNVAKLIGKMEKLVFATAELHSAMEVLVEMEASEKKMQKRK 180

Query: 181 AVTPKQCLPVNFELFDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTV 240
            +TPKQ +P+ F++FDKKLA+QRKDVKHFKEISLWNQSFDYAVGLMTRL+CVIYARI  V
Sbjct: 181 EITPKQGVPLKFDMFDKKLASQRKDVKHFKEISLWNQSFDYAVGLMTRLVCVIYARIFAV 240

Query: 241 FGSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKC--SDNDHRLVTQSGPIPKKGKKE 300
           F  FV DQ CSLDQNPQI+ L +RVWRWNF+G HRK    D++H+LVTQSGPIPK GKKE
Sbjct: 241 FRPFVSDQVCSLDQNPQIQNLSNRVWRWNFHGEHRKVGGGDDEHKLVTQSGPIPKNGKKE 300

Query: 301 LIRFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCL 360
           LIRFPSGIR K++  I YG F+SS A NNRVYTSAPPTTVGGSGLS+NYANVILFAERCL
Sbjct: 301 LIRFPSGIREKNNPRIEYGEFDSSMATNNRVYTSAPPTTVGGSGLSLNYANVILFAERCL 360

Query: 361 HAPATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWRE 420
           +   TIG++ARGELY+MLP RIK KVRAKL+RNNW KRG G+EEM S  DGHSLA GWRE
Sbjct: 361 NEATTIGDDARGELYQMLPARIKEKVRAKLRRNNWGKRGGGKEEMRSADDGHSLAVGWRE 420

Query: 421 AVEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVG 480
           A+EEMMGWLGPLAHDTVRWQSERNMEKQRFDT  T LLMQTLHYSDLEKTEAAIVEVLVG
Sbjct: 421 AMEEMMGWLGPLAHDTVRWQSERNMEKQRFDTGSTVLLMQTLHYSDLEKTEAAIVEVLVG 480

Query: 481 LSCIYRYENRRPQGRRSSD 498
           L CIYRYENRRPQ  RS D
Sbjct: 481 LCCIYRYENRRPQDHRSCD 499

BLAST of Spg012114 vs. ExPASy TrEMBL
Match: A0A6J1HN78 (uncharacterized protein LOC111466181 OS=Cucurbita maxima OX=3661 GN=LOC111466181 PE=4 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.3e-229
Identity = 398/500 (79.60%), Postives = 442/500 (88.40%), Query Frame = 0

Query: 1   MVVATMPWLSGSIKGTTGHTLQFFFHDKTPPPTLRILAFDTAKTMAALISLYRSLSDDEI 60
           MVVATMPWL+GS+KG + HTL FFF DKT PPTLRILAFDTAKTMAALISLYRSL+D EI
Sbjct: 1   MVVATMPWLTGSLKGASNHTLHFFFQDKTSPPTLRILAFDTAKTMAALISLYRSLTDKEI 60

Query: 61  NNLQKHALRSQGVLYLNSRNEDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120
           + L+ H + S+GV+YLNS +E+FLL LACSERLEELNNAASSVSRLSRKCADLGLTRFDL
Sbjct: 61  SKLRNHVVPSKGVVYLNSMDEEFLLNLACSERLEELNNAASSVSRLSRKCADLGLTRFDL 120

Query: 121 VFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWR 180
           VFSDMKLGIF+WGKSDSGFKNV KLIG+MEKLV+ TAELHSAMEVL+ MEASEKK+QK +
Sbjct: 121 VFSDMKLGIFNWGKSDSGFKNVAKLIGKMEKLVYSTAELHSAMEVLVGMEASEKKLQKRK 180

Query: 181 AVTPKQCLPVNFELFDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTV 240
            +TPKQ +P+ FE+FDKKLA+QRKDVKHFKEISLWNQSFDYAVGLMTRL+CVIYARI  V
Sbjct: 181 EITPKQGVPMKFEMFDKKLASQRKDVKHFKEISLWNQSFDYAVGLMTRLVCVIYARIFAV 240

Query: 241 FGSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKC-SDNDHRLVTQSGPIPKKGKKEL 300
           F  FV +Q CSLDQNPQIR L +RVWRWNF+G H+K   D++ +LVTQSGPIPK GKKEL
Sbjct: 241 FRPFVSEQVCSLDQNPQIRNLSNRVWRWNFHGEHQKAGGDDEQKLVTQSGPIPKNGKKEL 300

Query: 301 IRFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLH 360
           IRFPSGIR K++  I YG F+SS A NNRVYTSAPPTTVGGSGLS+NYANVILFA+RCL+
Sbjct: 301 IRFPSGIRDKNNPRIEYGEFDSSMATNNRVYTSAPPTTVGGSGLSLNYANVILFADRCLN 360

Query: 361 APATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREA 420
              TIG++ARGELY+MLP RIK KVRAKL+RNNW KRG G++EM S  DGHSLA GWREA
Sbjct: 361 EATTIGDDARGELYQMLPARIKEKVRAKLRRNNWGKRGGGKKEMRSADDGHSLAVGWREA 420

Query: 421 VEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGL 480
           +EEMMGWLGPLAHDTVRWQSERNMEKQRFDT  T LLMQTLHYSDLEKTEAAIVEVLVGL
Sbjct: 421 MEEMMGWLGPLAHDTVRWQSERNMEKQRFDTGSTVLLMQTLHYSDLEKTEAAIVEVLVGL 480

Query: 481 SCIYRYENRRPQGRRSSDHV 500
            CIYRYENRRPQ  RS DH+
Sbjct: 481 CCIYRYENRRPQDHRSCDHL 500

BLAST of Spg012114 vs. TAIR 10
Match: AT3G23160.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 256.5 bits (654), Expect = 4.3e-68
Identity = 174/495 (35.15%), Postives = 265/495 (53.54%), Query Frame = 0

Query: 28  KTPPP------TLRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRNE 87
           KTP P      T+ IL+F+ A  M+  I L+RSLSD EI+ L+     S+GV  L S +E
Sbjct: 31  KTPKPVSLPKQTIGILSFEVANVMSKTIHLHRSLSDTEISKLKAEVFHSEGVRKLVSSDE 90

Query: 88  DFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDLVFSDMKLGIFHWGKSDSGFKN 147
           + LL L+ SE+L++L+  AS VSRL +KC +  L  F+ V+ D+  G   + K     K+
Sbjct: 91  NHLLDLSVSEKLDDLSRVASVVSRLGKKCNEPALQGFEHVYEDIVNGAIDFRKLGFLVKD 150

Query: 148 VGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWRAVTPKQCLPVNFELFDKKLAT 207
           +  ++ +ME+ V  T  L+  MEV+ E+E +  K+Q+       Q    + + F++KL  
Sbjct: 151 MESMVKKMERFVNATCSLYCEMEVMNELEQAIVKLQR------SQQHQESVKAFEQKLMW 210

Query: 208 QRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTVFGSFVPDQSCSLDQNPQIRIL 267
           QR+DVK  ++ SLWNQ++D  V ++ R +C IY RI TVFG         L     + + 
Sbjct: 211 QRQDVKSLRDGSLWNQTYDKVVEMLARTVCTIYGRIETVFGGL------GLRGKKDVTLK 270

Query: 268 RDR-------VWRWNFYGGHRKCSDNDHRLVTQSGPI-------PKKGKKELIRFPSGIR 327
           RDR               G +    ++    T++G         P +   E +     I 
Sbjct: 271 RDRSKNEASKAVNSRSVAGFKDSRRSEADEFTRAGDFNFPCGTNPGRMFMECLAMNRTIG 330

Query: 328 AKDDIGIGYG---VFNSSTAA---------NNRVYTSAPPTTVGGSGLSMNYANVILFAE 387
             DD     G    F  STA           +R+   A  +T+GGS LS++YANV++  E
Sbjct: 331 DDDDDDDEDGGRITFPLSTARMIRSNKFGFKSRLTQHASASTIGGSALSLHYANVVIVVE 390

Query: 388 RCLHAPATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAG 447
           + L  P  IGEEAR +LY+MLPT +K  ++A L+  +++K  +  +          LA  
Sbjct: 391 KLLKYPHLIGEEARDDLYQMLPTSLKTTLKASLR--SYLKNISIYDA--------PLAHD 450

Query: 448 WREAVEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPT-ALLMQTLHYSDLEKTEAAIVE 490
           W+E ++ ++ WL PLAH+ +RWQSERN E+Q      T  LL+QTL+++D EKTEAAI +
Sbjct: 451 WKETIDGILSWLAPLAHNMIRWQSERNFEQQNQIVKRTNVLLLQTLYFADREKTEAAICK 503

BLAST of Spg012114 vs. TAIR 10
Match: AT5G04550.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 193.7 bits (491), Expect = 3.4e-49
Identity = 155/568 (27.29%), Postives = 259/568 (45.60%), Query Frame = 0

Query: 34  LRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRNEDFLLKLACSERL 93
           L +LAF+ A  ++ L+ L++SLSD  +  L+     S G+  L S ++DF+++L   E +
Sbjct: 26  LGVLAFEVASLLSKLVHLWQSLSDKNVARLRDEITHSTGIKKLVSEDDDFIVRLIRDEMM 85

Query: 94  EELNNAASSVSRLSRKCADLGLTRFDLVFSD-MKLGIFHWGKSDSGFKNVGKLIGRMEKL 153
           E + N A +V+RL+RKC D  L  F+  FSD MK G   +G    G+K + K   +ME+ 
Sbjct: 86  ENVENVAKAVARLARKCNDPKLKCFENCFSDMMKTGADPYG-WQFGWKKMDKKAKKMERF 145

Query: 154 VFLTAELHSAMEVLLEMEASEKKMQKWRAVTPKQCLPVNFELFDKKLATQRKDVKHFKEI 213
           +   A L+   E+L ++E + K+M+   + T       N   + KK+  +R +VK+ +++
Sbjct: 146 ISSNASLYQETEILADLEQTFKRMKSNESATD------NLLEYQKKVTWKRHEVKNLRDV 205

Query: 214 SLWNQSFDYAVGLMTRLICVIYARISTVFG------------------------------ 273
           SLWN+++DY V L+ R +  I +R   VFG                              
Sbjct: 206 SLWNRTYDYTVILLVRSVFTILSRTKHVFGISYRVEASDVSSADSDFIGRSHSVSTILTP 265

Query: 274 -------SFVP-----------------------------DQSCSLDQNPQIRILRDRVW 333
                  S +P                               S S +      ++ ++  
Sbjct: 266 VSHKSESSGLPRFASGPLGRFTGPASGSAATRSTKMSDFLSGSLSAESPKSGPLVAEKHK 325

Query: 334 RWNFYGGHRKCSDNDHRLVTQSGPIPKKGK--KELIRFP---------SGIRAKDDIGIG 393
           R+ FY G         ++ ++SGP+   GK  K++ + P           ++A     +G
Sbjct: 326 RFKFYSGQL------GKITSKSGPLIGMGKNNKKMGQTPERPSISSVKKQLKANRLTQVG 385

Query: 394 ---------YGVFNSSTAANNRVYTS-----------------------------APPTT 453
                     G+   ST A N    S                             A P T
Sbjct: 386 PFKGCMVSHDGITPLSTRARNGARNSSAEHHILEDNSNSVHVENLTLPSRPKLSDAAPNT 445

Query: 454 VGGSGLSMNYANVILFAERCLHAPATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRG 486
           +G + L+++YANVI+  ER + +P  IG++AR +LY MLP  ++  +R +LK   + K  
Sbjct: 446 LGTACLALHYANVIIVIERFVASPHLIGDDARDDLYNMLPASVRTSLRERLK--PYSKNL 505

BLAST of Spg012114 vs. TAIR 10
Match: AT5G51670.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 186.4 bits (472), Expect = 5.5e-47
Identity = 146/498 (29.32%), Postives = 233/498 (46.79%), Query Frame = 0

Query: 26  HDKTPP-----PTLRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRN 85
           H  +PP      ++ +L+F+ A+ M  L+ L  SL+D  +   + H+L  +G+  + + +
Sbjct: 24  HRSSPPISTTTSSVGVLSFEVARVMTKLLHLTHSLTDSNLLTPRDHSLSLEGLTKIVNGD 83

Query: 86  EDFLLKLACSERLEELNNAASSVSRLSRKCADLGLTRFDLV---FSDMKLGIFHW--GKS 145
           E F L L C+E  + L +AA+SVSRLS +C    L  F  +   F+DM      W     
Sbjct: 84  ETFHLSLVCAELADSLAHAANSVSRLSNRCTTASLRSFHRLFHEFADMGRDPHGWVMNCK 143

Query: 146 DSGFKNVGKLIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWRAVTPKQCLPVNFEL- 205
           D+  KN      ++E+ V +T  L+  ME +  +E S +          KQ L +  E  
Sbjct: 144 DTEAKN-----KKIERYVSVTTALYREMEEMAILENSLR----------KQSLQIGIEFE 203

Query: 206 ----------------FDKKLATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARIS 265
                              K+  Q++ VK+ K+ SLWN+SFD  V ++ R +    AR+ 
Sbjct: 204 EEEDYENKKDVMKVIDLQNKIERQKQHVKYLKDRSLWNKSFDTVVLILARSVFTALARLK 263

Query: 266 TVF------GSFVPDQSCSLDQNPQIRILRDRVWRWNFYGGHRKCSDNDHRLVTQSGPIP 325
           +VF      G  VP    SL      R L       N    H   +D +    T S    
Sbjct: 264 SVFSSAAATGYMVPTVVSSLP-----RSLSSSSSSMNLV--HPSPNDEERDKTTTSSAFL 323

Query: 326 KKGKKELIRFPSGIRAKDDIGIGYGVFNSSTAANNRVYTSAPPTTVGGSGLSMNYANVIL 385
           ++  + L                                  P TT+GG+G++++YAN+I+
Sbjct: 324 EESSRLL--------------------------------KPPETTLGGAGVALHYANLIV 383

Query: 386 FAERCLHAPATIGEEARGELYEMLPTRIKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSL 445
             E+ +  P  +G +AR +LY MLP  +++ +R++LK       G G      G     L
Sbjct: 384 VMEKMIKQPQLVGLDARDDLYSMLPASVRSSLRSRLK-------GVGFTATDGG-----L 443

Query: 446 AAGWREAVEEMMGWLGPLAHDTVRWQSERNMEKQRFDTSPTA----LLMQTLHYSDLEKT 487
           A  W+ A+  ++ WL PLA + +RWQSER+ E+Q   T+  +    +L+QTL ++D  KT
Sbjct: 444 ATEWKAALGRILRWLLPLAQNMIRWQSERSFEQQHMATATNSQNRVMLVQTLVFADKVKT 455

BLAST of Spg012114 vs. TAIR 10
Match: AT1G34320.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-19
Identity = 101/458 (22.05%), Postives = 189/458 (41.27%), Query Frame = 0

Query: 34  LRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRNEDFLLKLACSERL 93
           + IL+F+ A T+    +L  SLS D I +L++  L S+GV  L S++ D LL++A +++ 
Sbjct: 155 ISILSFEVANTIVKGANLMHSLSKDSITHLKEVVLPSEGVQNLISKDMDELLRIAAADKR 214

Query: 94  EELNNAASSVSRLSRKCADLGLTRFDLVFSDMKLGIFHWGKSDSGFKNVGKLIGRMEKLV 153
           EEL   +  V R   +C D      D  F   +LG   +       +    ++ +M   V
Sbjct: 215 EELRIFSGEVVRFGNRCKDPQYHNLDRFFD--RLG-SEFTPQKHLKQEAETIMHQMMSFV 274

Query: 154 FLTAELHSAMEVL--LEMEASEKKMQKWRAVTPKQCLPVNFELFDKKLATQRKDVKHFKE 213
             TA+L+  +  L   E +   K  ++    T ++ +     +   +L +Q+K V++ K+
Sbjct: 275 HFTADLYHELHALDRFEQDYQRKIQEEENPSTAQRGVGDTLAILRTELKSQKKHVRNLKK 334

Query: 214 ISLWNQSFDYAVGLMTRLICVIYARISTVFGSFVPDQSCSLDQNPQIRILRDRVWRWNFY 273
            SLW++  +  +  +  ++  ++  I   FG   PD+  +   +P I             
Sbjct: 335 KSLWSRILEEVMEKLVDVVHFLHLEIHEAFGGADPDKPAN---DPPI------------- 394

Query: 274 GGHRKCSDNDHRLVTQSGPIPKKGKKELIRFPSGIRAKDDIGIGYGVFNSSTAANNRVYT 333
             H+K                                                       
Sbjct: 395 -NHKK------------------------------------------------------- 454

Query: 334 SAPPTTVGGSGLSMNYANVILFAERCLHAPATIGEEARGELYEMLPTRIKAKVRAKLKRN 393
                 +G +GL+++YAN+I   +  +   +T+    R  LY+ LP  IK+ +R+++ ++
Sbjct: 455 ------LGSAGLALHYANIITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRI-QS 514

Query: 394 NWVKRGAGEEEMGSGGDGHSLAAGWREAVEEMMGWLGPLAHDTVR----------WQSER 453
             VK      ++             +  +E+ + WL P+A +T +          W S  
Sbjct: 515 FQVKEELTVPQI-------------KAEMEKTLQWLVPVATNTTKAHHGFGWVGEWASSG 516

Query: 454 NMEKQRFDTSPTALLMQTLHYSDLEKTEAAIVEVLVGL 480
           +   QR     T L + TLH++D EKTEA I++++V L
Sbjct: 575 SEANQR-PAGQTILRIDTLHHADKEKTEAYILDLVVWL 516

BLAST of Spg012114 vs. TAIR 10
Match: AT5G08660.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 85.1 bits (209), Expect = 1.7e-16
Identity = 101/469 (21.54%), Postives = 174/469 (37.10%), Query Frame = 0

Query: 34  LRILAFDTAKTMAALISLYRSLSDDEINNLQKHALRSQGVLYLNSRNEDFLLKLACSERL 93
           L ILAF+ A T+    +L  SLS   I +L+   L S+GV  L S + D LL+L  +++ 
Sbjct: 149 LGILAFEVANTIVKSSNLIESLSKRNIEHLKGTILYSEGVQNLVSNDFDELLRLVAADKR 208

Query: 94  EELNNAASSVSRLSRKCADLGLTRFDLVFSDMKLGIFHWGKSDSGFKNVGK--------- 153
           +EL   +  V R   +  D                 F W      F  + K         
Sbjct: 209 QELQVFSGEVVRFGNRSKD-----------------FQWHNLQRYFDRISKELTPQRQLK 268

Query: 154 -----LIGRMEKLVFLTAELHSAMEVLLEMEASEKKMQKWRAVTPKQCLPVNFELFDKKL 213
                ++ ++  LV  TAEL+  ++VL  +E   ++ ++    +          +   +L
Sbjct: 269 EDAVLVVDQLMVLVQYTAELYQELQVLYRLEKDYEQKRREEENSANSSKGDGLAILKTEL 328

Query: 214 ATQRKDVKHFKEISLWNQSFDYAVGLMTRLICVIYARISTVFGSFVPDQSCSLDQNPQIR 273
             QRK VK  K+ SLW++ F+  +  +  ++  +   I  +FG          D  P   
Sbjct: 329 KAQRKVVKSLKKKSLWSRGFEEVMEKLVDIVHFLLLEIHNIFG--------GADDQPS-- 388

Query: 274 ILRDRVWRWNFYGGHRKCSDNDHRLVTQSGPIPKKGKKELIRFPSGIRAKDDIGIGYGVF 333
                                            KKG  E  +                  
Sbjct: 389 ---------------------------------KKGAAEYDK------------------ 448

Query: 334 NSSTAANNRVYTSAPPTTVGGSGLSMNYANVILFAERCLHAPATIGEEARGELYEMLPTR 393
                             +G +GL+++YAN+I+  +  +   ++I   AR  LY+ LP  
Sbjct: 449 -----------------RLGPAGLALHYANIIVQIDTLVARASSITSNARDSLYQSLPPG 508

Query: 394 IKAKVRAKLKRNNWVKRGAGEEEMGSGGDGHSLAAGWREAVEEMMGWLGPLAHDTVR--- 453
           IK  +R+K+K  N               D        ++ +E  + WL P+A +T +   
Sbjct: 509 IKLALRSKIKSFN--------------VDKELSVTQIKDEMERTLHWLVPVAGNTTKAHH 508

Query: 454 ---WQSERNMEKQRFDTSPTA---LLMQTLHYSDLEKTEAAIVEVLVGL 480
              W  E       F + P+    L ++TL+++  EKTE  I+  ++ L
Sbjct: 569 GFGWVGEWANTGTDFTSKPSGGDILRIETLYHASKEKTEIYILGQIIWL 508