Spg012064 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012064
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAlpha-mannosidase
Locationscaffold1: 4619906 .. 4625430 (+)
RNA-Seq ExpressionSpg012064
SyntenySpg012064
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGACGAAGCAACATGCCATTACATAGACATTATTGACCAAACCACTCTCGGCCACAGTGTGATAAAGGAAGAGTTCGACAAGGTTCCTCGTGCTGGATGGCAGATTGATCCGTTTGGTCACTCTGCAGTGCAAGCTTACTTACTTGGAGCTGAGGTCCTTGGTTTCTTTTGTCTCCAATCCTAAAGTCCCTCCACATTTTCTTCCTTTTTTTTTACTATGTATTCACTTTTCTTTGATAGAATTGATATGGCCACGCTTTTTCTTAGAATGATGAATCACCTGTGTTTTAGTTCTTCTACCACTCGAACTTCCAACGCATAGTTCTGTACTTACATGTTTTCATTTTTCAGGTTGGTTTCGAGTCTGTACATTTTGCAAGAATTGATTATCAGGATAGAGAAAAGCGCAAGGTAGATAAATCTCTAGAAGTTGTATGGCGTGGCTCCAAAACTTTTGGTTCTTCATCTCAGGTGTACCATAACACATCAGATTTTCTATCTTCTTACAATGTCCATTCTTTCTTGTTATCTGCTGTTGCATTTATATACTTAATTTTGTGCTTACTGTCCGTTTATAGATTTTTGCAAATGCCTTCCCTGTACATTACAGTCCTCCTCCTGGTTTTCACTTTGAAGTGAACGACGACTCCAATCTTATTCAGGTATAATGCTTGTGAACGAAAGGCTACTCCAAATCCTTGGTAACAGTCACATTACCTCTTAACTAATATGATAAAACTGCAGGACGATCCTTTACTTTATGACTACAACGTTGAACAGCGGGTTAATGATTTTATAGATGCTGCCCTGACCCAAGTAAAATTTCCATTTATTGATTTGCATTCTGATTTTAGGATTTGGCCCAATAAGTGGTAATGACAAACGTTGGTTAATTTGCACTTTTGTTTATGTAGGCAAATATCACCCGGACGAATCATATTATGTGGACAATGGGTGACGATTTCCATACCAATATGCTGAGTCTTGGTTCCGGCAAATGGATAAGTTTATTCACTATGTTAAAAAGGTATGGTTGAATGAAAAATCAATTCATTTTTGGCATGTGTTTTGCATTCTGATTATTGGGTTTCCCGTTTCCCACTATGTTTTGTTGTTTTGGAATGCATAATTTACCAGGATGGTCGAATTAATGCTCTGTATTCAACACCATCCATATATACTGATGCAAAACACGCAGCAAATGAATCATGGCCATTGAAAACCGAGGATTATTTCCCGTGAGTGTTGCATCATCCTTCAAGTTTAATAACGTGCCCCCTATTGTGAATGAAGCATAATTTTCTTTTGGTATTATGTAATCGATCAATATCCTTATTACAGGTATGTTGATCGTGAACATGCGTACTGGACTGGCTTCTTTACAAGTCGCCCAGCCTTTAAAGGATATATCAGAATGCTGAGTGGATATTACTTGGTAAGTCATAAGGGCAGCTGAGGATTTATTAAGGAGTGACTAAACGTTGTACTTTAACTCTTGGAATGTCATAACCTCTAAACGTATTATCGGGCTCTTGAGTATAGCTGCTGAAGATCTTCTATTGTGATGCCAAGCAGAATCTGAATTGCTAGTTCATTGTTTTTGAATTGCTTGGTACATTTTTAAAATCCATATCAGCTGAATAATCTTTTGTTGCATATTAGGCAGCACGACAGCTCGAATTCCTTGTTGGAAGGAGATCTCGTGGCCCCAATACTTATAGTCTTGGTGATGCTTTGGGGATTGCACAACACCATGATGCAGTGACTGGTACTGCTAAGCAGCATACTACAAATGACTATATGAAACGGCTTGCTGCAGGATCCTCTGAGGTGGGTGTCATGTGTTTTGCTCGAGCCTTGGTTGGATCCTATAATTGCATTCTAGTTTACCCTTTTTTCTTCTTGTAACCTTGAATATTCTTAGCTTTTAATGTAAAGACAATGGAAACATTTACAGGTTGAAACAATGGTGAATTCTGCCCTGAATTGCCTGGGACAAAAGTGTAACGCCCCAGGCCCAAGATTCGAAATCCGGATTCGGCACCTCACGGCCCCGGCATTCCCCTACATCCTCTGCGGCCTAGCGACATCACCATACTTGTCTGAAACCGCTTCTAAGAGTGAAGACTGTCCTCACAAACCAACACGAGGTCCTTTTAGCATGCTTTGTCCTCACTCACATGCTTCCTAGGAAAATTCCCAGGAGGTCACCCAACATAGAACTTCTCCAAGCCAAGCACGCTTAACTTCAAAGTTCTTATGATTGAGCCACCAAAAAGGAAGGTGCACCTTGTTGGTATAGGTAGTATCTATCAATTCTTTTAAGCTTATCTTGACCATACTTTCATGTCCTCAGGATCCCTCTCATTCGGATGTGATCTCGGTTCATTCATGTACCCCTCCTAACTTCGGGCGTCACATGCCCACCAGCTTCCGCCTTGGTTCGTCCCCAAACCACATCCTACTAGGAGAGGTTTCGCTCTGATACCATCTGTAACGTCCCAGGCCCAGGATTCGGAATCTGTAACGCCCCAGGCCCAAGATTCGAAATCCGGATTCGGCACCTCACGACCCCGGCATTCCCCTACATCCTCTGCGGCCTGGCGACATCACCATACTTGTCTGAAACCGCTTCTAAGAGTGAAGACTGTCCCCACAAACCAACACGAGGTCCTTTTAGCATGCTTTGTCCTCACTCACATGCTTCCTAGGAAAATTCCCAGGAGGTCACCCAACATAGAACTTCTCCAAGCCAAGCACGCTTAACTTCAAAGTTCCTATGATTGAGCCACCGAAAAGGAAGGTGCATCTTGTTGGTATAGGTAGTATCTATCAATTCTTTTAAGCTTATCTTGACCATACTTTCATGTCCTCAGGATCCCTCTCATTCGGATGTGATCTCGGTTCATTCATGTACCCCTCCTAACTTCGGGCGTCACAAAAAGAAATTGGGAGGTCATTGTGAGAAATCAAAAATACTAAGCCAGGTGATGCTTTATTGTTGCTTGAATTTCTGATATTATTTATGAGGTTCATTTTCTCCTGAAATTTGTTCTTCATTTTCCGAACATTTGGATTCTTTTTGTCATTATTACTTTGGAATTGTTGCATTTTATGACTTTTGAAATTCCCTTTATTTTTTGTTAAGTAGTGTCCATTACTCAATATCAGTTTCTGCCCACCAACCGAGCAACATATTCAAGGAGGGAATAGTTTGGTAACGTTTCTTTCTTTCTCCAGTCTTCTGGAAATGTGCTTTTTACAATTTTTAACTACAATGTGAATGAGTAAATGTGCCTGTGGTCAGGTCGTGGTGGTGTATAACCCTCTTGGATGGATTCGTAATGATATCATTAGAATACCGGTGAGTAGAAACTTGCTTTTGCTTGCTTGTTTCACGTTCCAGACAATTGAATGACTTGGATTATTACTTCATCATTAGACCTTTTACAATTGAGGGAAATATGAATGGGAAGAGAAAAATGGAATCCATTCTATTCATGTAATCCGAAAATGAAAACTGTTATCAGGTTCATGATGCTGATCTTGTTGTCCAAGATACCTCAGGAAAAACCATTGAGACCCAATTTGTTGCCATTGATAAAGTCACAAAAAACATAAGCGAGTTGTATGTGAAGGCCTACTTGGGACAGCTGAGGGGAAATTTTCCAAAGTACTGGCTTCTGTTTCAAGTATCAGTCCCTCAACTTGGATGGAACACGTACTTTATTTCTAAAGGAAGTGGTGGAGGTAGATATTTAACTTTCTATCCTTGTCTGTCATTTTTCCTTTCTAGTCACTTAGAACAGTGGATGTTTCGAATTCAAGAGTTATGATCTCTCAACTGTGGTGAAATCTCACATTTTAATAGCCGGGTTTCTTTGAAGTGGATCTTAAGGTGTGCTCATATGCTGAGAAAACCATAGAAGTTCCATAAAAGAATTATAATATGGAACTTCTAATTCACTTTTGCACCAAAATTATTCAGGTAAAGGAAGATCTGAATTTCTTCCCGTGGACACTCAGAAGGATACCGTTGAAGTTGGACCTGGCAATTTGAAGATGTCCTTTTCTATTGCTTCTGGTCAACTCAAACGCGTGTACAATTCAAAGACCGGGGTAAATTGTCTCGCTTTTTCCTGTCAAAATACTTTTAGCATTAAAAGGTGGATATAAGATGGATGTTTAAAAAGTACTTCGTTCATCTTATACTGCTTAGATGGTTGGTTATATGCATTTTTGTTAGTTTCCATGTTCCAATGCATGTTTAACTTAATTTTGATTGATCTCGTGATCTTACCATAAGTGATATTTTCTCAGTTTTTGTTTGTTCTTTCTTTGGACTTTATGATGCTCTTTCCTTACAAAATTATCTGAGAATTCTCAATAAGATGAGCATCCTGTATATAATTATAGAGTTTTTCGGCTCAACGTCCTAATTGCTGTTTATTTTTGTTTCTAATACTTACTTTGGTTCCACTTGAGTTAAATTAGAATAATCTTATAGTTGTTATCTCAATACGCCAGGTTGATGTACCAATACAACAAAGCTATCTTTGGTATGCTTCAGCTACAGGTTTCTTTAATAATGATCAGGTCGGTCTCTCTTAAAACAACGAAATTTCATGAAATTGAAATTTGGCATCTACTAAATAGGTCTTCCATAACTTCTTGTAGAATTATGGTGCATATATTTTTGTGCCTCAAGGGGAACCAAATATCGTTTCCAGATCAGTAAGCCTTATGTTTCCGTTGTCACTTTCGTATGTAATGTGCATCAAGTCGTTAACTTCCTGTATTTATTATGTTCCACACTTCTTTTTCCTCATTTTTCATATTGAACTTGACAGGTTGCTGTGGAAATCGTTCGTGGACCGCTAGTTGATGAGGTCCGCCAACAATTCAGTCCATGGGTTTACCAGGTTGCTTACTAAAAGTAGAATGAAGTTGAAAAAGGAATTGTCCATTTGGGTTCATATCTGTTAATGGCCTAATCTTTGAGGATATGGTTGTGCATTTGGTCGTTAGACTTTACAAAGACAAAGAGCATGCTGAATTTGAATTCACTGTGAGTATGTCAAAACTTTATTCTCCAATTGCCTCACTTTTCATCTTCTGTTTGTTAAAGTCCAAAACTAGTTGATACCTTAAGCTTCCAATTGAATGGGTTTTGTGATTTTTTACTCTGAAGGTTGGTCCAATTCCAGTGGATGATAGTGTGGGAAAGGAGGTCATCACAAGATTGACAACAAACATGGTTACAGATAAGGCGTTTTACACTGATTCGAACGGAAGAGACTTTTTGAAGCGGGTAATTAGCACACAAGTATTGAAATTTCAGTTGAATGTCTTTCAATAACATTTGAATTATCATAATCTCTCCTTGTTGTTTTGAGAACATTTTCCTTTTCTCTCTTATTCTGCACCATTTCTCTTCTCACCTTCAGTTGTTGTCATGAGTAATATTTGCAGGTTCGAGACTATAGACAAGACTGGAATCTTTCTGTCAATGAACCGTAG

mRNA sequence

ATGCATGACGAAGCAACATGCCATTACATAGACATTATTGACCAAACCACTCTCGGCCACAGTGTGATAAAGGAAGAGTTCGACAAGGTTCCTCGTGCTGGATGGCAGATTGATCCGTTTGGTCACTCTGCAGTGCAAGCTTACTTACTTGGAGCTGAGGTTGGTTTCGAGTCTGTACATTTTGCAAGAATTGATTATCAGGATAGAGAAAAGCGCAAGGTAGATAAATCTCTAGAAGTTGTATGGCGTGGCTCCAAAACTTTTGGTTCTTCATCTCAGATTTTTGCAAATGCCTTCCCTGTACATTACAGTCCTCCTCCTGGTTTTCACTTTGAAGTGAACGACGACTCCAATCTTATTCAGGACGATCCTTTACTTTATGACTACAACGTTGAACAGCGGGTTAATGATTTTATAGATGCTGCCCTGACCCAAGATGGTCGAATTAATGCTCTGTATTCAACACCATCCATATATACTGATGCAAAACACGCAGCAAATGAATCATGGCCATTGAAAACCGAGGATTATTTCCCGTATGTTGATCGTGAACATGCGTACTGGACTGGCTTCTTTACAAGTCGCCCAGCCTTTAAAGGATATATCAGAATGCTGAGTGGATATTACTTGGCAGCACGACAGCTCGAATTCCTTGTTGGAAGGAGATCTCGTGGCCCCAATACTTATAGTCTTGGTGATGCTTTGGGGATTGCACAACACCATGATGCAGTGACTGGTACTGCTAAGCAGCATACTACAAATGACTATATGAAACGGCTTGCTGCAGGATCCTCTGAGGTCGTGGTGGTGTATAACCCTCTTGGATGGATTCGTAATGATATCATTAGAATACCGGTTCATGATGCTGATCTTGTTGTCCAAGATACCTCAGGAAAAACCATTGAGACCCAATTTGTTGCCATTGATAAAGTCACAAAAAACATAAGCGAGTTGTATGTGAAGGCCTACTTGGGACAGCTGAGGGGAAATTTTCCAAAGTACTGGCTTCTGTTTCAAGTATCAGTCCCTCAACTTGGATGGAACACGTACTTTATTTCTAAAGGAAGTGGTGGAGGTAAAGGAAGATCTGAATTTCTTCCCGTGGACACTCAGAAGGATACCGTTGAAGTTGGACCTGGCAATTTGAAGATGTCCTTTTCTATTGCTTCTGGTCAACTCAAACGCGTGTACAATTCAAAGACCGGGGTTGATGTACCAATACAACAAAGCTATCTTTGGTATGCTTCAGCTACAGGGGAACCAAATATCGTTTCCAGATCAGTAAGCCTTATGTTTCCGTTGTCACTTTCGTATGTTGCTGTGGAAATCGTTCGTGGACCGCTAGTTGATGAGGTCCGCCAACAATTCAGTCCATGGGTTTACCAGGTTGGTCCAATTCCAGTGGATGATAGTGTGGGAAAGGAGGTCATCACAAGATTGACAACAAACATGGTTACAGATAAGGCGTTTTACACTGATTCGAACGGAAGAGACTTTTTGAAGCGGGTTCGAGACTATAGACAAGACTGGAATCTTTCTGTCAATGAACCGTAG

Coding sequence (CDS)

ATGCATGACGAAGCAACATGCCATTACATAGACATTATTGACCAAACCACTCTCGGCCACAGTGTGATAAAGGAAGAGTTCGACAAGGTTCCTCGTGCTGGATGGCAGATTGATCCGTTTGGTCACTCTGCAGTGCAAGCTTACTTACTTGGAGCTGAGGTTGGTTTCGAGTCTGTACATTTTGCAAGAATTGATTATCAGGATAGAGAAAAGCGCAAGGTAGATAAATCTCTAGAAGTTGTATGGCGTGGCTCCAAAACTTTTGGTTCTTCATCTCAGATTTTTGCAAATGCCTTCCCTGTACATTACAGTCCTCCTCCTGGTTTTCACTTTGAAGTGAACGACGACTCCAATCTTATTCAGGACGATCCTTTACTTTATGACTACAACGTTGAACAGCGGGTTAATGATTTTATAGATGCTGCCCTGACCCAAGATGGTCGAATTAATGCTCTGTATTCAACACCATCCATATATACTGATGCAAAACACGCAGCAAATGAATCATGGCCATTGAAAACCGAGGATTATTTCCCGTATGTTGATCGTGAACATGCGTACTGGACTGGCTTCTTTACAAGTCGCCCAGCCTTTAAAGGATATATCAGAATGCTGAGTGGATATTACTTGGCAGCACGACAGCTCGAATTCCTTGTTGGAAGGAGATCTCGTGGCCCCAATACTTATAGTCTTGGTGATGCTTTGGGGATTGCACAACACCATGATGCAGTGACTGGTACTGCTAAGCAGCATACTACAAATGACTATATGAAACGGCTTGCTGCAGGATCCTCTGAGGTCGTGGTGGTGTATAACCCTCTTGGATGGATTCGTAATGATATCATTAGAATACCGGTTCATGATGCTGATCTTGTTGTCCAAGATACCTCAGGAAAAACCATTGAGACCCAATTTGTTGCCATTGATAAAGTCACAAAAAACATAAGCGAGTTGTATGTGAAGGCCTACTTGGGACAGCTGAGGGGAAATTTTCCAAAGTACTGGCTTCTGTTTCAAGTATCAGTCCCTCAACTTGGATGGAACACGTACTTTATTTCTAAAGGAAGTGGTGGAGGTAAAGGAAGATCTGAATTTCTTCCCGTGGACACTCAGAAGGATACCGTTGAAGTTGGACCTGGCAATTTGAAGATGTCCTTTTCTATTGCTTCTGGTCAACTCAAACGCGTGTACAATTCAAAGACCGGGGTTGATGTACCAATACAACAAAGCTATCTTTGGTATGCTTCAGCTACAGGGGAACCAAATATCGTTTCCAGATCAGTAAGCCTTATGTTTCCGTTGTCACTTTCGTATGTTGCTGTGGAAATCGTTCGTGGACCGCTAGTTGATGAGGTCCGCCAACAATTCAGTCCATGGGTTTACCAGGTTGGTCCAATTCCAGTGGATGATAGTGTGGGAAAGGAGGTCATCACAAGATTGACAACAAACATGGTTACAGATAAGGCGTTTTACACTGATTCGAACGGAAGAGACTTTTTGAAGCGGGTTCGAGACTATAGACAAGACTGGAATCTTTCTGTCAATGAACCGTAG

Protein sequence

MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVHFARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLIQDDPLLYDYNVEQRVNDFIDAALTQDGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYIRMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAAGSSEVVVVYNPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNFPKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDTQKDTVEVGPGNLKMSFSIASGQLKRVYNSKTGVDVPIQQSYLWYASATGEPNIVSRSVSLMFPLSLSYVAVEIVRGPLVDEVRQQFSPWVYQVGPIPVDDSVGKEVITRLTTNMVTDKAFYTDSNGRDFLKRVRDYRQDWNLSVNEP
Homology
BLAST of Spg012064 vs. NCBI nr
Match: XP_023545016.1 (alpha-mannosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.1 bits (2330), Expect = 2.2e-258
Identity = 467/641 (72.85%), Postives = 488/641 (76.13%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEATCHYID+IDQTTLGHSVIKEEF+ VPRAGWQIDPFGHSAVQAYLLGAEVGF+SVH
Sbjct: 137 MHDEATCHYIDMIDQTTLGHSVIKEEFNTVPRAGWQIDPFGHSAVQAYLLGAEVGFDSVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDR KR VDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVND++N+I
Sbjct: 197 FARIDYQDRAKRMVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDNTNII 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDPLLYDYNV+QRVNDFIDAALTQ                                   
Sbjct: 257 QDDPLLYDYNVKQRVNDFIDAALTQANVTRTNHIMWTMGDDFQYQYAESWFKQMDKFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPS+YTDAK+AANESWPLKT+D+FPY DREHAYWTGFFTSRPAFKGYI
Sbjct: 317 VKKDGRINALYSTPSVYTDAKNAANESWPLKTDDFFPYADREHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           RMLSGYYLAARQLEFLVGRRS GPNTYSLGDALGIAQHHDAVTGTAKQHTTNDY KRLAA
Sbjct: 377 RMLSGYYLAARQLEFLVGRRSNGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYTKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   VVVVY
Sbjct: 437 GSSEAEAVVNSALICLGQKKLGDHCEISKTLSQCPLLNISFCPPTEQDIQEGNSLVVVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRND +RIPVHDADLVVQD SGKTIE+QF+AIDKVTKNISE YVKAY G+ RG F
Sbjct: 497 NPLGWIRNDFVRIPVHDADLVVQDISGKTIESQFLAIDKVTKNISEFYVKAYSGRARGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDT--QKDTVEVGPGNLKMSFSIA 480
           PKYWLLFQVSVPQLGWNTYFISKGSGGGK RS FLP DT  QKDT+ VGPG+LKMSFSIA
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGSGGGKRRSNFLPTDTDSQKDTIVVGPGHLKMSFSIA 616

Query: 481 SGQLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMF 518
           SGQLKR+YNSKTGVDVPIQQSYLWYASAT                 GEPNI+SRSV +  
Sbjct: 617 SGQLKRMYNSKTGVDVPIQQSYLWYASATGSINNNQNSGAYIFVPQGEPNIISRSVPM-- 676

BLAST of Spg012064 vs. NCBI nr
Match: XP_038883639.1 (alpha-mannosidase [Benincasa hispida])

HSP 1 Score: 900.2 bits (2325), Expect = 8.3e-258
Identity = 466/639 (72.93%), Postives = 486/639 (76.06%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEAT HYID+IDQTTLGHSVIK+EF+KVPRAGWQIDPFGHSAVQAYLL AEVGF+SVH
Sbjct: 137 MHDEATSHYIDMIDQTTLGHSVIKQEFNKVPRAGWQIDPFGHSAVQAYLLSAEVGFDSVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDR KRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI
Sbjct: 197 FARIDYQDRAKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDP LYDYNVE+RVNDFIDAAL Q                                   
Sbjct: 257 QDDPSLYDYNVEERVNDFIDAALNQANITRTNHIMWTMGDDFQYQYAESWFRQMDKFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPSIYTDAKHAANESWPLKT+D+FPY DREHAYWTGFFTSRPAFKGYI
Sbjct: 317 VKKDGRINALYSTPSIYTDAKHAANESWPLKTDDFFPYADREHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           RMLSGYYLAARQLEFLVGR+S  PNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA
Sbjct: 377 RMLSGYYLAARQLEFLVGRKSGSPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   V+VVY
Sbjct: 437 GSSEAEAVVNSALICLGQKQLGDHCEQSKILSQCPLLNISFCPPTEQDIQEGNSLVMVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRNDI+RIPVHDADLVVQD SGKTIETQFVA+DKVT NISE YVKA LG  RG F
Sbjct: 497 NPLGWIRNDIVRIPVHDADLVVQDISGKTIETQFVAVDKVTNNISEFYVKANLGLPRGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDTQKDTVEVGPGNLKMSFSIASG 480
           PKYWLLFQVSVPQLGWNTYFISKGSGGGK RS+FLP D+Q DT+EVGPG LKMSFSIASG
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGSGGGKRRSKFLPADSQNDTIEVGPGQLKMSFSIASG 616

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMFPL 518
           QLKR+YNSKTGVDVPIQQSYLWYAS++                 GEPNI+SRS       
Sbjct: 617 QLKRMYNSKTGVDVPIQQSYLWYASSSGFYSDEQNSGAYIFVPQGEPNILSRS------- 676

BLAST of Spg012064 vs. NCBI nr
Match: XP_022950420.1 (alpha-mannosidase [Cucurbita moschata])

HSP 1 Score: 898.7 bits (2321), Expect = 2.4e-257
Identity = 467/641 (72.85%), Postives = 488/641 (76.13%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEATCHYID+IDQTTLGHSVIKEEF+ VPRAGWQIDPFGHSAVQAYLLGAEVGF+SVH
Sbjct: 137 MHDEATCHYIDMIDQTTLGHSVIKEEFNTVPRAGWQIDPFGHSAVQAYLLGAEVGFDSVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDR KR VDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVND++N+I
Sbjct: 197 FARIDYQDRAKRMVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDNTNII 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDPLLYDYNV+QRVNDFIDAALTQ                                   
Sbjct: 257 QDDPLLYDYNVKQRVNDFIDAALTQANVTRTNHIMWTMGDDFQYQYAESWFKQMDKFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPS+YTDAK+AANESWPLKT+D+FPY DR+HAYWTGFFTSRPAFKGYI
Sbjct: 317 VKKDGRINALYSTPSMYTDAKNAANESWPLKTDDFFPYADRDHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           RMLSGYYLAARQLEFLVGRRS GPNTYSLGDALGIAQHHDAVTGTAKQHTTNDY KRLAA
Sbjct: 377 RMLSGYYLAARQLEFLVGRRSSGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYTKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   VVVVY
Sbjct: 437 GSSEAEAVVNSALICLGQKKLGDHCEISKTLSQCPLLNISFCPPTEQDIQGGNSLVVVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRND +RIPVHDADLVVQD SGKTIE+QF+AIDKVTKNISE YVKAY GQ RG F
Sbjct: 497 NPLGWIRNDFVRIPVHDADLVVQDISGKTIESQFLAIDKVTKNISEFYVKAYSGQARGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDT--QKDTVEVGPGNLKMSFSIA 480
           PKYWLLFQVSVPQLGWNTYFISKGS GGK RS+FLP DT  QKDTV VGPG+LKMSFSIA
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGSDGGKTRSKFLPTDTDSQKDTVVVGPGHLKMSFSIA 616

Query: 481 SGQLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMF 518
           SGQLKR+YNSKTGVDVPIQQSYLWYASA+                 GEPNI+SRS     
Sbjct: 617 SGQLKRMYNSKTGVDVPIQQSYLWYASASGSINNNQNSGAYIFVPQGEPNIISRS----- 676

BLAST of Spg012064 vs. NCBI nr
Match: XP_022977427.1 (alpha-mannosidase [Cucurbita maxima])

HSP 1 Score: 897.5 bits (2318), Expect = 5.4e-257
Identity = 466/641 (72.70%), Postives = 486/641 (75.82%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEATCHYID+IDQTTLGHSVIKEEF+ VPRAGWQIDPFGHSAVQAYLLGAEVGF+SVH
Sbjct: 137 MHDEATCHYIDMIDQTTLGHSVIKEEFNTVPRAGWQIDPFGHSAVQAYLLGAEVGFDSVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDR KR VDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVND++N+I
Sbjct: 197 FARIDYQDRAKRMVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDNTNII 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDPLLYDYNV+QRVNDFIDAALTQ                                   
Sbjct: 257 QDDPLLYDYNVKQRVNDFIDAALTQANVTRTNHIMWTMGDDFQYQYAESWFKQMDKFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPSIYTDAK+AANESWPLKT+D+FPY DREHAYWTGFFTSRPAFKGYI
Sbjct: 317 VKKDGRINALYSTPSIYTDAKNAANESWPLKTDDFFPYADREHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           RMLSGYYLAARQLEFLVGRRS GPNTYSLGDALGIAQHHDAVTGTAKQHTTNDY KRLAA
Sbjct: 377 RMLSGYYLAARQLEFLVGRRSSGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYTKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   VVVVY
Sbjct: 437 GSSEAEAVVNSALVCLGQKKLGDHCEISKTLSQCPLLNISFCPPTEQDIQEGNSLVVVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRNDI+RIPVHDADLVVQD SGKTIE+QF+AID VTKNISE YVKAY G+ RG F
Sbjct: 497 NPLGWIRNDIVRIPVHDADLVVQDISGKTIESQFLAIDNVTKNISEFYVKAYSGRARGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDT--QKDTVEVGPGNLKMSFSIA 480
           PKYWLLFQVSVPQLGWNTYFISKGSGGGK RS+FLP DT  QKDTV VGPG+LKMSFSIA
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGSGGGKRRSKFLPTDTDSQKDTVVVGPGHLKMSFSIA 616

Query: 481 SGQLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMF 518
           SGQLKR+YNSKTGVDVPIQQSYLWYASAT                 GEPNI+SRSV +  
Sbjct: 617 SGQLKRMYNSKTGVDVPIQQSYLWYASATGSINNNQNSGAYIFVPQGEPNIISRSVPM-- 676

BLAST of Spg012064 vs. NCBI nr
Match: XP_004135022.1 (alpha-mannosidase [Cucumis sativus] >KGN48931.1 hypothetical protein Csa_003885 [Cucumis sativus])

HSP 1 Score: 895.2 bits (2312), Expect = 2.7e-256
Identity = 464/639 (72.61%), Postives = 486/639 (76.06%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEAT HYID+IDQTTLGHSVIKEEF+KVPRAGWQIDPFGHSAVQAYLL AEVGFESVH
Sbjct: 137 MHDEATSHYIDMIDQTTLGHSVIKEEFNKVPRAGWQIDPFGHSAVQAYLLSAEVGFESVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIF NAFPVHYSPPPGFHFEV+DDSNLI
Sbjct: 197 FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFTNAFPVHYSPPPGFHFEVDDDSNLI 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDP LYDYNVEQRVNDFI+AALTQ                                   
Sbjct: 257 QDDPSLYDYNVEQRVNDFIEAALTQANITRTNHIMWTMGDDFQYQYAESWFRQMDRFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPSIYTDAKHAANESWPLKT+D+FPY DR+HAYWTGFFTSRPAFKGYI
Sbjct: 317 IKKDGRINALYSTPSIYTDAKHAANESWPLKTDDFFPYADRDHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           R LSGYYLAARQLEFLVGR+S GPNT+SLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA
Sbjct: 377 RTLSGYYLAARQLEFLVGRKSGGPNTFSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   V+VVY
Sbjct: 437 GSSEAEAVVNSALICLGQRQLGDHCEQPKILSQCPLLNISFCPPTEQDIQEGNSLVMVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRND +RIPVHDADLVVQD SGKTIETQFVAIDKVT ++SE YVKA LG  RG F
Sbjct: 497 NPLGWIRNDFVRIPVHDADLVVQDISGKTIETQFVAIDKVTNSVSEFYVKANLGLPRGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDTQKDTVEVGPGNLKMSFSIASG 480
           PKYWLLFQVSVPQLGWNTYFISKGSGGGK RSEF P+D+Q DT+EVGPG+LKMSFSIASG
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGSGGGKRRSEFFPMDSQNDTIEVGPGHLKMSFSIASG 616

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMFPL 518
           QLKR+YNSKTGVD+PIQQSYLWY S+T                 GEPNIVSRS    FP 
Sbjct: 617 QLKRMYNSKTGVDLPIQQSYLWYTSSTGFYSDQQNSGAYIFVPQGEPNIVSRS----FP- 676

BLAST of Spg012064 vs. ExPASy Swiss-Prot
Match: Q9FKW9 (Probable alpha-mannosidase At5g66150 OS=Arabidopsis thaliana OX=3702 GN=At5g66150 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 4.4e-185
Identity = 339/651 (52.07%), Postives = 407/651 (62.52%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           M+DEATCHYID+IDQTT GH  IK++F+  PRA WQIDPFGHS+VQAYLLGAE+G +SVH
Sbjct: 141 MNDEATCHYIDMIDQTTKGHRFIKQQFNTTPRAAWQIDPFGHSSVQAYLLGAELGLDSVH 200

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDREKRK +KSLEV+WRGSKT  SSSQIF N F VHY PP GFH+EV DD   +
Sbjct: 201 FARIDYQDREKRKAEKSLEVIWRGSKTLDSSSQIFTNIFFVHYGPPTGFHYEVTDDYVPL 260

Query: 121 QDDPLLYDYNVEQRVNDFIDAAL------------------------------------- 180
           QD+P    YN+++ VNDF++A+L                                     
Sbjct: 261 QDNPRFDGYNIKEAVNDFVNASLVYANVSRGNHVMWTMGDDFQYQFAESWFRQMDRLIHY 320

Query: 181 -TQDGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
             +DGR+NALYSTPS+Y DAK+ AN +WPLKT D+FPY DR +AYWTG+FTSRPA K Y+
Sbjct: 321 VNKDGRVNALYSTPSLYVDAKNVANVTWPLKTHDFFPYADRAYAYWTGYFTSRPALKRYV 380

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           R LSGYY+AARQLEFLVG+ S GPNTYSLGDALGIAQHHDAVTGTAKQH TNDYMKRLA 
Sbjct: 381 RALSGYYMAARQLEFLVGKNSGGPNTYSLGDALGIAQHHDAVTGTAKQHVTNDYMKRLAL 440

Query: 301 GSSE-----------------------------------------------------VVV 360
           G+SE                                                     ++V
Sbjct: 441 GASEAEAVVNSALACLMNKAPKGGCTKPAIAFSQQCSLMNISYCPSTEETLPGQKSLILV 500

Query: 361 VYNPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRG 420
            YN LGW R +IIRIPV+DA L V+D+SG T++ Q++ +D VT N+   Y KAYLG    
Sbjct: 501 AYNSLGWNRTEIIRIPVNDAGLSVEDSSGNTLDAQYIPMDNVTSNLRSFYTKAYLGISSL 560

Query: 421 NFPKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPV--DTQKDTVEVGPGNLKMSFS 480
             PKYWL+F+  VP LGWNT+FISK S  G    +   V      +T E+GPGNLKM FS
Sbjct: 561 QRPKYWLVFKAKVPPLGWNTFFISKASAQGSNNHKHSSVMLSPMNNTTEIGPGNLKMVFS 620

Query: 481 IASGQLKRVYNSKTGVDVPIQQSYLWYASATGEPN--------IVSRSVSLMFPLSLSYV 518
             SG+L+R+YNS+TG D+ + Q+Y WYAS  G+          I   + SL +P+S S +
Sbjct: 621 SDSGRLERMYNSRTGADIKVDQNYFWYASNVGDAKDPQVSGAYIFRPNGSLAYPVSSSKI 680

BLAST of Spg012064 vs. ExPASy Swiss-Prot
Match: C0HJB3 (Alpha-mannosidase OS=Canavalia ensiformis OX=3823 PE=1 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 2.2e-184
Identity = 336/631 (53.25%), Postives = 411/631 (65.13%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEAT HYID+ID TTLGH  ++E+F+K+PRAGWQIDPFGHSAVQ YLLGAE+GF+SVH
Sbjct: 108 MHDEATTHYIDMIDHTTLGHRFLQEQFNKIPRAGWQIDPFGHSAVQGYLLGAELGFDSVH 167

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDREKRK +KSLEVVWRGSKTFGSS+QIFANAFP HY PP GF+FEV ++   +
Sbjct: 168 FARIDYQDREKRKGEKSLEVVWRGSKTFGSSAQIFANAFPGHYGPPNGFNFEVRNNFVPL 227

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDP L+D NVE+RV +F+DAALTQ                                   
Sbjct: 228 QDDPRLFDTNVEERVQNFLDAALTQAKLTRTNHLMWTMGDDFQYQYAESWFKQMDKLLHH 287

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGR+NALYSTPS+YT+AK+AAN++WPLK +DYFPY D  +AYWTGF+TSR       
Sbjct: 288 VNKDGRVNALYSTPSLYTEAKNAANQTWPLKIDDYFPYADGRNAYWTGFYTSRXXXXXXX 347

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
            MLSGYYLA R   F  G++S   + + L DALGIAQHHDAV+GTAKQHTTNDY KRLA 
Sbjct: 348 XMLSGYYLATRHSGFFAGKKSTKYHAFDLADALGIAQHHDAVSGTAKQHTTNDYAKRLAL 407

Query: 301 GSSE----------------------------------------------------VVVV 360
           G+S+                                                    VVVV
Sbjct: 408 GASKAEAVVSSSLACLTSKQSADQCSAPASAFSQCHLFNISYCPPTESSLPDDKSLVVVV 467

Query: 361 YNPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGN 420
           YNPLGW RN+I+RIPV+DA+LVV+D+SG  +E Q+V +D VT N+   YVK         
Sbjct: 468 YNPLGWSRNEIVRIPVNDANLVVKDSSGNKLEVQYVEMDDVTANLRSFYVKXXXXXXXXX 527

Query: 421 FPKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDTQKDTVEVGPGNLKMSFSIAS 480
              YW LF+ SVP LGW+TYFIS+ +  GKG    L +  + +T+ +GPG+LKMSFS  +
Sbjct: 528 XXXYWSLFKASVPPLGWSTYFISEAT--GKGTRNALTLSQKGETLNIGPGDLKMSFSSLT 587

Query: 481 GQLKRVYNSKTGVDVPIQQSYLWYASATGEPNIVSRSVSLMF-------PLSLSYVAV-E 518
           GQLKR+YNSKTGVD+PIQQ+YLWY S+ G+ +    S + +F       P ++S  +V  
Sbjct: 588 GQLKRMYNSKTGVDLPIQQNYLWYESSEGDFSDYQASGAYLFRPNGQPPPHTVSRSSVTR 647

BLAST of Spg012064 vs. ExPASy Swiss-Prot
Match: Q8LPJ3 (Probable alpha-mannosidase At5g13980 OS=Arabidopsis thaliana OX=3702 GN=At5g13980 PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.5e-164
Identity = 308/633 (48.66%), Postives = 393/633 (62.09%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEA  HYID+IDQTTLGH  I  EF+  PR GWQIDPFGHSAVQAYLLGAEVGF+SV 
Sbjct: 131 MHDEAAPHYIDMIDQTTLGHRFIIREFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVF 190

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPP-GFHFEVNDDSNL 120
           F RIDYQDREKR  +K+LEV+WRGSK+ GSSSQIFA AFP +Y PPP GF++E+ DDS +
Sbjct: 191 FGRIDYQDREKRYKEKTLEVIWRGSKSLGSSSQIFAGAFPTNYEPPPGGFYYEITDDSPV 250

Query: 121 IQDDPLLYDYNVEQRVNDFIDAALTQ---------------------------------- 180
           +QDDP L+DYNV++RVN F+ AAL Q                                  
Sbjct: 251 VQDDPDLFDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRYQYAHTWYRQMDKLIH 310

Query: 181 ----DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGY 240
               DGR+NA YSTPSIYTDAKHAANE+WPLKTEDYFPY DR +AYWTG+FTSRPA K Y
Sbjct: 311 YVNLDGRVNAFYSTPSIYTDAKHAANEAWPLKTEDYFPYADRINAYWTGYFTSRPALKRY 370

Query: 241 IRMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLA 300
           +R++S YYLAARQLEF  GR  +GPNT SL DAL IAQHHDAV+GT+KQH  NDY KRLA
Sbjct: 371 VRVMSAYYLAARQLEFFKGRSQKGPNTDSLADALAIAQHHDAVSGTSKQHVANDYAKRLA 430

Query: 301 AG--------------------------------------SSEV---------VVVYNPL 360
            G                                      SSEV         V+ YNPL
Sbjct: 431 IGYVEAESVVATSLAHLTKVDPTLNPTFQQCLLLNISYCPSSEVNLSDGKSLIVLAYNPL 490

Query: 361 GWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNFPKY 420
           GW R DI+R+PV   D+ V D+ G  +E+Q V        + + +V+AYLGQ     PKY
Sbjct: 491 GWKRVDIVRLPVVGGDVSVHDSEGHEVESQLVPFTDEYVALRKYHVEAYLGQSPTQVPKY 550

Query: 421 WLLFQVSVPQLGWNTYFIS--KGSGGGKGRSEFLPV-DTQKDTVEVGPGNLKMSFSIASG 480
           WL+F V+VP LG+ TY IS  K + G   +S    +   ++  + +G G+LK+SFS   G
Sbjct: 551 WLVFSVTVPPLGFTTYTISTAKKTDGYSSKSYVSNILKGEQSIINIGHGHLKLSFSTDQG 610

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASATG----EPNIVSRSVSLMFPLSLSY-------VA 518
                 N +T +  P++Q++ +Y++  G    EP I   S + +F  + ++       V 
Sbjct: 611 TAINYVNGRTSMTEPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVP 670

BLAST of Spg012064 vs. ExPASy Swiss-Prot
Match: P94078 (Alpha-mannosidase At3g26720 OS=Arabidopsis thaliana OX=3702 GN=At3g26720 PE=1 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 4.6e-158
Identity = 302/631 (47.86%), Postives = 378/631 (59.90%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEAT HYID+IDQTTLGH  IK EF +VPR GWQIDPFGHSAVQAYLLGAE GF+S+ 
Sbjct: 132 MHDEATPHYIDMIDQTTLGHQFIKTEFGQVPRVGWQIDPFGHSAVQAYLLGAEFGFDSLF 191

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDR KR  +K+LEV+W+GSK+ GSSSQIF   FP HY PP GF FE+ND S  I
Sbjct: 192 FARIDYQDRAKRLREKTLEVIWQGSKSLGSSSQIFTGVFPRHYDPPEGFTFEINDVSAPI 251

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDPLL+DYNV++RVNDF+ AAL Q                                   
Sbjct: 252 QDDPLLFDYNVQERVNDFVAAALAQVNVTRTNHIMWLMGTDFRYQYAYSWFRQIDKFIHY 311

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGR+N LYSTPSIYTDAK+AANESWPLKT+D+FPY D+ +AYWTG+FTSRPAFK Y+
Sbjct: 312 VNKDGRLNVLYSTPSIYTDAKYAANESWPLKTDDFFPYADKPNAYWTGYFTSRPAFKKYV 371

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLA- 300
           R LSGYYLAARQLEFL GR S GP T  L DAL IAQHHDAV+GT +QH   DY  RL+ 
Sbjct: 372 RDLSGYYLAARQLEFLRGRDSSGPTTDMLADALAIAQHHDAVSGTQRQHVAADYALRLSM 431

Query: 301 ---------------------------------------------------AGSSEVVVV 360
                                                              +G S VVVV
Sbjct: 432 GYLQAEKLVASSLSFLSAAKSSTEKKNPGTKFQQCPLLNISYCPASEARLLSGKSLVVVV 491

Query: 361 YNPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGN 420
           YN LGW R +++R+PV   +++V+D SGK +  Q + + ++   I   YVKAYLG+   +
Sbjct: 492 YNSLGWKREEVVRVPVSSENVIVKDASGKEVVFQLLPLSEIALRIRNEYVKAYLGRSPRD 551

Query: 421 FPKYWLLFQVSVPQLGWNTYFISKGSGGGKGRS-EFLPVDTQKDTVEVGPGNLKMSFSIA 480
             K+ L F  SVP LG+++Y IS      +G S  ++   +    VEVG GNLK+ +S  
Sbjct: 552 TAKHVLAFTASVPPLGFSSYVISDTGRTARGLSASYVTSGSMNQNVEVGQGNLKLRYSEE 611

Query: 481 SGQLKRVYNSKTGVDVPIQQSYLWYASATGEPNIVSRSVSLMF------PL-SLSYVAVE 518
             ++ R  ++K    V  +QSY +Y  + G       S + +F      P+ S     + 
Sbjct: 612 GVKITRHLSTKN--QVTAEQSYAYYIGSNGTDKDPQASGAYVFRPDGVLPIKSKEEAQLT 671

BLAST of Spg012064 vs. ExPASy Swiss-Prot
Match: P34098 (Lysosomal alpha-mannosidase OS=Dictyostelium discoideum OX=44689 GN=manA PE=1 SV=2)

HSP 1 Score: 314.7 bits (805), Expect = 2.0e-84
Identity = 205/663 (30.92%), Postives = 305/663 (46.00%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           M+DEAT +Y D IDQ TLGH  + E F  +P+ GW IDPFGHSA QA + G ++GF++  
Sbjct: 136 MNDEATTYYDDTIDQMTLGHQFLWENFGVMPKIGWHIDPFGHSATQARIFG-QLGFDAFI 195

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
             R+DYQD E R  +K +E +WR +++    +Q+F +     Y  P GF+FE  DD   I
Sbjct: 196 IGRMDYQDIEARLENKQMEFMWRSTQS-TPENQVFTSVLRAMYCTPDGFNFEQGDDP--I 255

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQDGR-------------------------------- 180
           QDDP L+D NV+ R   F   AL                                     
Sbjct: 256 QDDPNLFDNNVDSRAEQFTQVALEYATHYRTNNVLIPFGCDFAYLNAQMYYKNIDKLIAH 315

Query: 181 ---------INALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFK 240
                    +N LYSTPSIY DA + AN  W +KT+D FPY D E +YWTG+F SRPA K
Sbjct: 316 INSNPDKYGLNLLYSTPSIYIDAVNDANLVWEVKTDDLFPYADNEFSYWTGYFVSRPALK 375

Query: 241 GYIRMLSGYYLAARQLEFLVGRRSRGPNTYS---------LGDALGIAQHHDAVTGTAKQ 300
           GY+R  +       Q+  LV   +  P++ S         + + +GIAQHHDAV+GT +Q
Sbjct: 376 GYVRQNNALLHVVEQM--LVTSSNLMPSSRSEQLVDDIVIMREVMGIAQHHDAVSGTEQQ 435

Query: 301 HTTNDYMKR--------------------------------------------------- 360
           H  +DY +R                                                   
Sbjct: 436 HVADDYAERLSIGNCASLETINTVVGTLLTANGNSKSAAATPTISFCPLLNQSICPATDP 495

Query: 361 LAAGSSEVVVVYNPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELY 420
           L++G+S  V++YN L W RN+ +RIP+  A++ V  +S  +I +Q               
Sbjct: 496 LSSGTSVPVLIYNSLSWTRNEPVRIPIPIANVTVTSSSNGSITSQ--------------- 555

Query: 421 VKAYLGQLRGNFPKYWLLFQVSVPQLGWNTYFISKG---------------------SGG 480
               + Q+ G F    L F  ++P LG++TY I+                       SGG
Sbjct: 556 ----VNQINGTF---ILEFLATIPPLGYSTYIITSTASDFVEPNSIPAIIIQDEIIVSGG 615

Query: 481 GKGRSEFLPVDTQKDTVEVGPGNLKMSFSIASGQLKRVYNSKTGVDVPIQQSYLWYASAT 518
           GK   +     +  D + +    + + FS   G +  + N  +GV   I Q Y+WY  + 
Sbjct: 616 GKINEKV----SYNDPIILENDYINVQFSSQDGSILSITNKTSGVTSSITQEYIWYNPSV 675

BLAST of Spg012064 vs. ExPASy TrEMBL
Match: A0A6J1GFN9 (Alpha-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111453525 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.2e-257
Identity = 467/641 (72.85%), Postives = 488/641 (76.13%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEATCHYID+IDQTTLGHSVIKEEF+ VPRAGWQIDPFGHSAVQAYLLGAEVGF+SVH
Sbjct: 137 MHDEATCHYIDMIDQTTLGHSVIKEEFNTVPRAGWQIDPFGHSAVQAYLLGAEVGFDSVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDR KR VDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVND++N+I
Sbjct: 197 FARIDYQDRAKRMVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDNTNII 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDPLLYDYNV+QRVNDFIDAALTQ                                   
Sbjct: 257 QDDPLLYDYNVKQRVNDFIDAALTQANVTRTNHIMWTMGDDFQYQYAESWFKQMDKFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPS+YTDAK+AANESWPLKT+D+FPY DR+HAYWTGFFTSRPAFKGYI
Sbjct: 317 VKKDGRINALYSTPSMYTDAKNAANESWPLKTDDFFPYADRDHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           RMLSGYYLAARQLEFLVGRRS GPNTYSLGDALGIAQHHDAVTGTAKQHTTNDY KRLAA
Sbjct: 377 RMLSGYYLAARQLEFLVGRRSSGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYTKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   VVVVY
Sbjct: 437 GSSEAEAVVNSALICLGQKKLGDHCEISKTLSQCPLLNISFCPPTEQDIQGGNSLVVVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRND +RIPVHDADLVVQD SGKTIE+QF+AIDKVTKNISE YVKAY GQ RG F
Sbjct: 497 NPLGWIRNDFVRIPVHDADLVVQDISGKTIESQFLAIDKVTKNISEFYVKAYSGQARGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDT--QKDTVEVGPGNLKMSFSIA 480
           PKYWLLFQVSVPQLGWNTYFISKGS GGK RS+FLP DT  QKDTV VGPG+LKMSFSIA
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGSDGGKTRSKFLPTDTDSQKDTVVVGPGHLKMSFSIA 616

Query: 481 SGQLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMF 518
           SGQLKR+YNSKTGVDVPIQQSYLWYASA+                 GEPNI+SRS     
Sbjct: 617 SGQLKRMYNSKTGVDVPIQQSYLWYASASGSINNNQNSGAYIFVPQGEPNIISRS----- 676

BLAST of Spg012064 vs. ExPASy TrEMBL
Match: A0A6J1IIE4 (Alpha-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111477773 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.6e-257
Identity = 466/641 (72.70%), Postives = 486/641 (75.82%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEATCHYID+IDQTTLGHSVIKEEF+ VPRAGWQIDPFGHSAVQAYLLGAEVGF+SVH
Sbjct: 137 MHDEATCHYIDMIDQTTLGHSVIKEEFNTVPRAGWQIDPFGHSAVQAYLLGAEVGFDSVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDR KR VDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVND++N+I
Sbjct: 197 FARIDYQDRAKRMVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDNTNII 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDPLLYDYNV+QRVNDFIDAALTQ                                   
Sbjct: 257 QDDPLLYDYNVKQRVNDFIDAALTQANVTRTNHIMWTMGDDFQYQYAESWFKQMDKFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPSIYTDAK+AANESWPLKT+D+FPY DREHAYWTGFFTSRPAFKGYI
Sbjct: 317 VKKDGRINALYSTPSIYTDAKNAANESWPLKTDDFFPYADREHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           RMLSGYYLAARQLEFLVGRRS GPNTYSLGDALGIAQHHDAVTGTAKQHTTNDY KRLAA
Sbjct: 377 RMLSGYYLAARQLEFLVGRRSSGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYTKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   VVVVY
Sbjct: 437 GSSEAEAVVNSALVCLGQKKLGDHCEISKTLSQCPLLNISFCPPTEQDIQEGNSLVVVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRNDI+RIPVHDADLVVQD SGKTIE+QF+AID VTKNISE YVKAY G+ RG F
Sbjct: 497 NPLGWIRNDIVRIPVHDADLVVQDISGKTIESQFLAIDNVTKNISEFYVKAYSGRARGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDT--QKDTVEVGPGNLKMSFSIA 480
           PKYWLLFQVSVPQLGWNTYFISKGSGGGK RS+FLP DT  QKDTV VGPG+LKMSFSIA
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGSGGGKRRSKFLPTDTDSQKDTVVVGPGHLKMSFSIA 616

Query: 481 SGQLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMF 518
           SGQLKR+YNSKTGVDVPIQQSYLWYASAT                 GEPNI+SRSV +  
Sbjct: 617 SGQLKRMYNSKTGVDVPIQQSYLWYASATGSINNNQNSGAYIFVPQGEPNIISRSVPM-- 676

BLAST of Spg012064 vs. ExPASy TrEMBL
Match: A0A0A0KMH9 (Alpha-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G507060 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.3e-256
Identity = 464/639 (72.61%), Postives = 486/639 (76.06%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEAT HYID+IDQTTLGHSVIKEEF+KVPRAGWQIDPFGHSAVQAYLL AEVGFESVH
Sbjct: 137 MHDEATSHYIDMIDQTTLGHSVIKEEFNKVPRAGWQIDPFGHSAVQAYLLSAEVGFESVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIF NAFPVHYSPPPGFHFEV+DDSNLI
Sbjct: 197 FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFTNAFPVHYSPPPGFHFEVDDDSNLI 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDP LYDYNVEQRVNDFI+AALTQ                                   
Sbjct: 257 QDDPSLYDYNVEQRVNDFIEAALTQANITRTNHIMWTMGDDFQYQYAESWFRQMDRFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPSIYTDAKHAANESWPLKT+D+FPY DR+HAYWTGFFTSRPAFKGYI
Sbjct: 317 IKKDGRINALYSTPSIYTDAKHAANESWPLKTDDFFPYADRDHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           R LSGYYLAARQLEFLVGR+S GPNT+SLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA
Sbjct: 377 RTLSGYYLAARQLEFLVGRKSGGPNTFSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   V+VVY
Sbjct: 437 GSSEAEAVVNSALICLGQRQLGDHCEQPKILSQCPLLNISFCPPTEQDIQEGNSLVMVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRND +RIPVHDADLVVQD SGKTIETQFVAIDKVT ++SE YVKA LG  RG F
Sbjct: 497 NPLGWIRNDFVRIPVHDADLVVQDISGKTIETQFVAIDKVTNSVSEFYVKANLGLPRGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDTQKDTVEVGPGNLKMSFSIASG 480
           PKYWLLFQVSVPQLGWNTYFISKGSGGGK RSEF P+D+Q DT+EVGPG+LKMSFSIASG
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGSGGGKRRSEFFPMDSQNDTIEVGPGHLKMSFSIASG 616

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMFPL 518
           QLKR+YNSKTGVD+PIQQSYLWY S+T                 GEPNIVSRS    FP 
Sbjct: 617 QLKRMYNSKTGVDLPIQQSYLWYTSSTGFYSDQQNSGAYIFVPQGEPNIVSRS----FP- 676

BLAST of Spg012064 vs. ExPASy TrEMBL
Match: A0A5D3CQ32 (Alpha-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001170 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 460/639 (71.99%), Postives = 483/639 (75.59%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEAT HYID+IDQTTLGHSVIKEEF+KVPRAGWQIDPFGHSAVQAYLL AEVGFESVH
Sbjct: 137 MHDEATSHYIDMIDQTTLGHSVIKEEFNKVPRAGWQIDPFGHSAVQAYLLSAEVGFESVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIF NAFPVHYSPPPGFHFEVNDDSNLI
Sbjct: 197 FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFTNAFPVHYSPPPGFHFEVNDDSNLI 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDP LYDYNVEQRVNDFIDAALTQ                                   
Sbjct: 257 QDDPSLYDYNVEQRVNDFIDAALTQANITRTNHIMWTMGDDFQYQYAESWFRQMDKFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPSIYTDAKHAANESWPLKT+D+FPY DR HAYWTGFFTSRPAFKGYI
Sbjct: 317 IKKDGRINALYSTPSIYTDAKHAANESWPLKTDDFFPYADRAHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           R LSGYYLAARQLEFLVGR+S GPNT+SLGDALG+AQHHDAVTGTAKQHTTNDYMKRLAA
Sbjct: 377 RTLSGYYLAARQLEFLVGRKSGGPNTFSLGDALGVAQHHDAVTGTAKQHTTNDYMKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   V+VVY
Sbjct: 437 GSSEAEAVVNSALICLGQKQLGDHCEQSKILSQCPLLNISFCPPTEQDIQKGNSLVMVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRND++RIPVHDADLVVQD SGKTIETQFVAIDKVT +ISE YVKA LG  RG F
Sbjct: 497 NPLGWIRNDVVRIPVHDADLVVQDISGKTIETQFVAIDKVTNSISEFYVKANLGLPRGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDTQKDTVEVGPGNLKMSFSIASG 480
           PKYWLLFQVSVPQLGWNTYFISKG+GGGK RSEF P D+Q DT+EVGPG+LKMSFS ASG
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGNGGGKRRSEFFPTDSQNDTIEVGPGHLKMSFSNASG 616

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMFPL 518
           QLKR++NSKTGVD+PIQQSYLWY S+T                 GEPNI+SRS       
Sbjct: 617 QLKRMFNSKTGVDLPIQQSYLWYTSSTGFFSDQQNSGAYIFVPQGEPNILSRS------- 676

BLAST of Spg012064 vs. ExPASy TrEMBL
Match: A0A1S3B2P6 (Alpha-mannosidase OS=Cucumis melo OX=3656 GN=LOC103485145 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 460/639 (71.99%), Postives = 483/639 (75.59%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEAT HYID+IDQTTLGHSVIKEEF+KVPRAGWQIDPFGHSAVQAYLL AEVGFESVH
Sbjct: 137 MHDEATSHYIDMIDQTTLGHSVIKEEFNKVPRAGWQIDPFGHSAVQAYLLSAEVGFESVH 196

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIF NAFPVHYSPPPGFHFEVNDDSNLI
Sbjct: 197 FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFTNAFPVHYSPPPGFHFEVNDDSNLI 256

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDP LYDYNVEQRVNDFIDAALTQ                                   
Sbjct: 257 QDDPSLYDYNVEQRVNDFIDAALTQANITRTNHIMWTMGDDFQYQYAESWFRQMDKFIHY 316

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGRINALYSTPSIYTDAKHAANESWPLKT+D+FPY DR HAYWTGFFTSRPAFKGYI
Sbjct: 317 IKKDGRINALYSTPSIYTDAKHAANESWPLKTDDFFPYADRAHAYWTGFFTSRPAFKGYI 376

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           R LSGYYLAARQLEFLVGR+S GPNT+SLGDALG+AQHHDAVTGTAKQHTTNDYMKRLAA
Sbjct: 377 RTLSGYYLAARQLEFLVGRKSGGPNTFSLGDALGVAQHHDAVTGTAKQHTTNDYMKRLAA 436

Query: 301 GSSE---------------------------------------------------VVVVY 360
           GSSE                                                   V+VVY
Sbjct: 437 GSSEAEAVVNSALICLGQKQLGDHCEQSKILSQCPLLNISFCPPTEQDIQKGNSLVMVVY 496

Query: 361 NPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNF 420
           NPLGWIRND++RIPVHDADLVVQD SGKTIETQFVAIDKVT +ISE YVKA LG  RG F
Sbjct: 497 NPLGWIRNDVVRIPVHDADLVVQDISGKTIETQFVAIDKVTNSISEFYVKANLGLPRGQF 556

Query: 421 PKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPVDTQKDTVEVGPGNLKMSFSIASG 480
           PKYWLLFQVSVPQLGWNTYFISKG+GGGK RSEF P D+Q DT+EVGPG+LKMSFS ASG
Sbjct: 557 PKYWLLFQVSVPQLGWNTYFISKGNGGGKRRSEFFPTDSQNDTIEVGPGHLKMSFSNASG 616

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASAT-----------------GEPNIVSRSVSLMFPL 518
           QLKR++NSKTGVD+PIQQSYLWY S+T                 GEPNI+SRS       
Sbjct: 617 QLKRMFNSKTGVDLPIQQSYLWYTSSTGFFSDQQNSGAYIFVPQGEPNILSRS------- 676

BLAST of Spg012064 vs. TAIR 10
Match: AT5G66150.1 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 649.0 bits (1673), Expect = 3.1e-186
Identity = 339/651 (52.07%), Postives = 407/651 (62.52%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           M+DEATCHYID+IDQTT GH  IK++F+  PRA WQIDPFGHS+VQAYLLGAE+G +SVH
Sbjct: 141 MNDEATCHYIDMIDQTTKGHRFIKQQFNTTPRAAWQIDPFGHSSVQAYLLGAELGLDSVH 200

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDREKRK +KSLEV+WRGSKT  SSSQIF N F VHY PP GFH+EV DD   +
Sbjct: 201 FARIDYQDREKRKAEKSLEVIWRGSKTLDSSSQIFTNIFFVHYGPPTGFHYEVTDDYVPL 260

Query: 121 QDDPLLYDYNVEQRVNDFIDAAL------------------------------------- 180
           QD+P    YN+++ VNDF++A+L                                     
Sbjct: 261 QDNPRFDGYNIKEAVNDFVNASLVYANVSRGNHVMWTMGDDFQYQFAESWFRQMDRLIHY 320

Query: 181 -TQDGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
             +DGR+NALYSTPS+Y DAK+ AN +WPLKT D+FPY DR +AYWTG+FTSRPA K Y+
Sbjct: 321 VNKDGRVNALYSTPSLYVDAKNVANVTWPLKTHDFFPYADRAYAYWTGYFTSRPALKRYV 380

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLAA 300
           R LSGYY+AARQLEFLVG+ S GPNTYSLGDALGIAQHHDAVTGTAKQH TNDYMKRLA 
Sbjct: 381 RALSGYYMAARQLEFLVGKNSGGPNTYSLGDALGIAQHHDAVTGTAKQHVTNDYMKRLAL 440

Query: 301 GSSE-----------------------------------------------------VVV 360
           G+SE                                                     ++V
Sbjct: 441 GASEAEAVVNSALACLMNKAPKGGCTKPAIAFSQQCSLMNISYCPSTEETLPGQKSLILV 500

Query: 361 VYNPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRG 420
            YN LGW R +IIRIPV+DA L V+D+SG T++ Q++ +D VT N+   Y KAYLG    
Sbjct: 501 AYNSLGWNRTEIIRIPVNDAGLSVEDSSGNTLDAQYIPMDNVTSNLRSFYTKAYLGISSL 560

Query: 421 NFPKYWLLFQVSVPQLGWNTYFISKGSGGGKGRSEFLPV--DTQKDTVEVGPGNLKMSFS 480
             PKYWL+F+  VP LGWNT+FISK S  G    +   V      +T E+GPGNLKM FS
Sbjct: 561 QRPKYWLVFKAKVPPLGWNTFFISKASAQGSNNHKHSSVMLSPMNNTTEIGPGNLKMVFS 620

Query: 481 IASGQLKRVYNSKTGVDVPIQQSYLWYASATGEPN--------IVSRSVSLMFPLSLSYV 518
             SG+L+R+YNS+TG D+ + Q+Y WYAS  G+          I   + SL +P+S S +
Sbjct: 621 SDSGRLERMYNSRTGADIKVDQNYFWYASNVGDAKDPQVSGAYIFRPNGSLAYPVSSSKI 680

BLAST of Spg012064 vs. TAIR 10
Match: AT5G13980.1 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 580.9 bits (1496), Expect = 1.0e-165
Identity = 308/633 (48.66%), Postives = 393/633 (62.09%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEA  HYID+IDQTTLGH  I  EF+  PR GWQIDPFGHSAVQAYLLGAEVGF+SV 
Sbjct: 131 MHDEAAPHYIDMIDQTTLGHRFIIREFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVF 190

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPP-GFHFEVNDDSNL 120
           F RIDYQDREKR  +K+LEV+WRGSK+ GSSSQIFA AFP +Y PPP GF++E+ DDS +
Sbjct: 191 FGRIDYQDREKRYKEKTLEVIWRGSKSLGSSSQIFAGAFPTNYEPPPGGFYYEITDDSPV 250

Query: 121 IQDDPLLYDYNVEQRVNDFIDAALTQ---------------------------------- 180
           +QDDP L+DYNV++RVN F+ AAL Q                                  
Sbjct: 251 VQDDPDLFDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRYQYAHTWYRQMDKLIH 310

Query: 181 ----DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGY 240
               DGR+NA YSTPSIYTDAKHAANE+WPLKTEDYFPY DR +AYWTG+FTSRPA K Y
Sbjct: 311 YVNLDGRVNAFYSTPSIYTDAKHAANEAWPLKTEDYFPYADRINAYWTGYFTSRPALKRY 370

Query: 241 IRMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLA 300
           +R++S YYLAARQLEF  GR  +GPNT SL DAL IAQHHDAV+GT+KQH  NDY KRLA
Sbjct: 371 VRVMSAYYLAARQLEFFKGRSQKGPNTDSLADALAIAQHHDAVSGTSKQHVANDYAKRLA 430

Query: 301 AG--------------------------------------SSEV---------VVVYNPL 360
            G                                      SSEV         V+ YNPL
Sbjct: 431 IGYVEAESVVATSLAHLTKVDPTLNPTFQQCLLLNISYCPSSEVNLSDGKSLIVLAYNPL 490

Query: 361 GWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNFPKY 420
           GW R DI+R+PV   D+ V D+ G  +E+Q V        + + +V+AYLGQ     PKY
Sbjct: 491 GWKRVDIVRLPVVGGDVSVHDSEGHEVESQLVPFTDEYVALRKYHVEAYLGQSPTQVPKY 550

Query: 421 WLLFQVSVPQLGWNTYFIS--KGSGGGKGRSEFLPV-DTQKDTVEVGPGNLKMSFSIASG 480
           WL+F V+VP LG+ TY IS  K + G   +S    +   ++  + +G G+LK+SFS   G
Sbjct: 551 WLVFSVTVPPLGFTTYTISTAKKTDGYSSKSYVSNILKGEQSIINIGHGHLKLSFSTDQG 610

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASATG----EPNIVSRSVSLMFPLSLSY-------VA 518
                 N +T +  P++Q++ +Y++  G    EP I   S + +F  + ++       V 
Sbjct: 611 TAINYVNGRTSMTEPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVP 670

BLAST of Spg012064 vs. TAIR 10
Match: AT5G13980.2 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 580.9 bits (1496), Expect = 1.0e-165
Identity = 308/633 (48.66%), Postives = 393/633 (62.09%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEA  HYID+IDQTTLGH  I  EF+  PR GWQIDPFGHSAVQAYLLGAEVGF+SV 
Sbjct: 131 MHDEAAPHYIDMIDQTTLGHRFIIREFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVF 190

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPP-GFHFEVNDDSNL 120
           F RIDYQDREKR  +K+LEV+WRGSK+ GSSSQIFA AFP +Y PPP GF++E+ DDS +
Sbjct: 191 FGRIDYQDREKRYKEKTLEVIWRGSKSLGSSSQIFAGAFPTNYEPPPGGFYYEITDDSPV 250

Query: 121 IQDDPLLYDYNVEQRVNDFIDAALTQ---------------------------------- 180
           +QDDP L+DYNV++RVN F+ AAL Q                                  
Sbjct: 251 VQDDPDLFDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRYQYAHTWYRQMDKLIH 310

Query: 181 ----DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGY 240
               DGR+NA YSTPSIYTDAKHAANE+WPLKTEDYFPY DR +AYWTG+FTSRPA K Y
Sbjct: 311 YVNLDGRVNAFYSTPSIYTDAKHAANEAWPLKTEDYFPYADRINAYWTGYFTSRPALKRY 370

Query: 241 IRMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLA 300
           +R++S YYLAARQLEF  GR  +GPNT SL DAL IAQHHDAV+GT+KQH  NDY KRLA
Sbjct: 371 VRVMSAYYLAARQLEFFKGRSQKGPNTDSLADALAIAQHHDAVSGTSKQHVANDYAKRLA 430

Query: 301 AG--------------------------------------SSEV---------VVVYNPL 360
            G                                      SSEV         V+ YNPL
Sbjct: 431 IGYVEAESVVATSLAHLTKVDPTLNPTFQQCLLLNISYCPSSEVNLSDGKSLIVLAYNPL 490

Query: 361 GWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNFPKY 420
           GW R DI+R+PV   D+ V D+ G  +E+Q V        + + +V+AYLGQ     PKY
Sbjct: 491 GWKRVDIVRLPVVGGDVSVHDSEGHEVESQLVPFTDEYVALRKYHVEAYLGQSPTQVPKY 550

Query: 421 WLLFQVSVPQLGWNTYFIS--KGSGGGKGRSEFLPV-DTQKDTVEVGPGNLKMSFSIASG 480
           WL+F V+VP LG+ TY IS  K + G   +S    +   ++  + +G G+LK+SFS   G
Sbjct: 551 WLVFSVTVPPLGFTTYTISTAKKTDGYSSKSYVSNILKGEQSIINIGHGHLKLSFSTDQG 610

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASATG----EPNIVSRSVSLMFPLSLSY-------VA 518
                 N +T +  P++Q++ +Y++  G    EP I   S + +F  + ++       V 
Sbjct: 611 TAINYVNGRTSMTEPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVP 670

BLAST of Spg012064 vs. TAIR 10
Match: AT5G13980.3 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 580.9 bits (1496), Expect = 1.0e-165
Identity = 308/633 (48.66%), Postives = 393/633 (62.09%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEA  HYID+IDQTTLGH  I  EF+  PR GWQIDPFGHSAVQAYLLGAEVGF+SV 
Sbjct: 131 MHDEAAPHYIDMIDQTTLGHRFIIREFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVF 190

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPP-GFHFEVNDDSNL 120
           F RIDYQDREKR  +K+LEV+WRGSK+ GSSSQIFA AFP +Y PPP GF++E+ DDS +
Sbjct: 191 FGRIDYQDREKRYKEKTLEVIWRGSKSLGSSSQIFAGAFPTNYEPPPGGFYYEITDDSPV 250

Query: 121 IQDDPLLYDYNVEQRVNDFIDAALTQ---------------------------------- 180
           +QDDP L+DYNV++RVN F+ AAL Q                                  
Sbjct: 251 VQDDPDLFDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRYQYAHTWYRQMDKLIH 310

Query: 181 ----DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGY 240
               DGR+NA YSTPSIYTDAKHAANE+WPLKTEDYFPY DR +AYWTG+FTSRPA K Y
Sbjct: 311 YVNLDGRVNAFYSTPSIYTDAKHAANEAWPLKTEDYFPYADRINAYWTGYFTSRPALKRY 370

Query: 241 IRMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLA 300
           +R++S YYLAARQLEF  GR  +GPNT SL DAL IAQHHDAV+GT+KQH  NDY KRLA
Sbjct: 371 VRVMSAYYLAARQLEFFKGRSQKGPNTDSLADALAIAQHHDAVSGTSKQHVANDYAKRLA 430

Query: 301 AG--------------------------------------SSEV---------VVVYNPL 360
            G                                      SSEV         V+ YNPL
Sbjct: 431 IGYVEAESVVATSLAHLTKVDPTLNPTFQQCLLLNISYCPSSEVNLSDGKSLIVLAYNPL 490

Query: 361 GWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGNFPKY 420
           GW R DI+R+PV   D+ V D+ G  +E+Q V        + + +V+AYLGQ     PKY
Sbjct: 491 GWKRVDIVRLPVVGGDVSVHDSEGHEVESQLVPFTDEYVALRKYHVEAYLGQSPTQVPKY 550

Query: 421 WLLFQVSVPQLGWNTYFIS--KGSGGGKGRSEFLPV-DTQKDTVEVGPGNLKMSFSIASG 480
           WL+F V+VP LG+ TY IS  K + G   +S    +   ++  + +G G+LK+SFS   G
Sbjct: 551 WLVFSVTVPPLGFTTYTISTAKKTDGYSSKSYVSNILKGEQSIINIGHGHLKLSFSTDQG 610

Query: 481 QLKRVYNSKTGVDVPIQQSYLWYASATG----EPNIVSRSVSLMFPLSLSY-------VA 518
                 N +T +  P++Q++ +Y++  G    EP I   S + +F  + ++       V 
Sbjct: 611 TAINYVNGRTSMTEPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVP 670

BLAST of Spg012064 vs. TAIR 10
Match: AT3G26720.1 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 559.3 bits (1440), Expect = 3.2e-159
Identity = 302/631 (47.86%), Postives = 378/631 (59.90%), Query Frame = 0

Query: 1   MHDEATCHYIDIIDQTTLGHSVIKEEFDKVPRAGWQIDPFGHSAVQAYLLGAEVGFESVH 60
           MHDEAT HYID+IDQTTLGH  IK EF +VPR GWQIDPFGHSAVQAYLLGAE GF+S+ 
Sbjct: 132 MHDEATPHYIDMIDQTTLGHQFIKTEFGQVPRVGWQIDPFGHSAVQAYLLGAEFGFDSLF 191

Query: 61  FARIDYQDREKRKVDKSLEVVWRGSKTFGSSSQIFANAFPVHYSPPPGFHFEVNDDSNLI 120
           FARIDYQDR KR  +K+LEV+W+GSK+ GSSSQIF   FP HY PP GF FE+ND S  I
Sbjct: 192 FARIDYQDRAKRLREKTLEVIWQGSKSLGSSSQIFTGVFPRHYDPPEGFTFEINDVSAPI 251

Query: 121 QDDPLLYDYNVEQRVNDFIDAALTQ----------------------------------- 180
           QDDPLL+DYNV++RVNDF+ AAL Q                                   
Sbjct: 252 QDDPLLFDYNVQERVNDFVAAALAQVNVTRTNHIMWLMGTDFRYQYAYSWFRQIDKFIHY 311

Query: 181 ---DGRINALYSTPSIYTDAKHAANESWPLKTEDYFPYVDREHAYWTGFFTSRPAFKGYI 240
              DGR+N LYSTPSIYTDAK+AANESWPLKT+D+FPY D+ +AYWTG+FTSRPAFK Y+
Sbjct: 312 VNKDGRLNVLYSTPSIYTDAKYAANESWPLKTDDFFPYADKPNAYWTGYFTSRPAFKKYV 371

Query: 241 RMLSGYYLAARQLEFLVGRRSRGPNTYSLGDALGIAQHHDAVTGTAKQHTTNDYMKRLA- 300
           R LSGYYLAARQLEFL GR S GP T  L DAL IAQHHDAV+GT +QH   DY  RL+ 
Sbjct: 372 RDLSGYYLAARQLEFLRGRDSSGPTTDMLADALAIAQHHDAVSGTQRQHVAADYALRLSM 431

Query: 301 ---------------------------------------------------AGSSEVVVV 360
                                                              +G S VVVV
Sbjct: 432 GYLQAEKLVASSLSFLSAAKSSTEKKNPGTKFQQCPLLNISYCPASEARLLSGKSLVVVV 491

Query: 361 YNPLGWIRNDIIRIPVHDADLVVQDTSGKTIETQFVAIDKVTKNISELYVKAYLGQLRGN 420
           YN LGW R +++R+PV   +++V+D SGK +  Q + + ++   I   YVKAYLG+   +
Sbjct: 492 YNSLGWKREEVVRVPVSSENVIVKDASGKEVVFQLLPLSEIALRIRNEYVKAYLGRSPRD 551

Query: 421 FPKYWLLFQVSVPQLGWNTYFISKGSGGGKGRS-EFLPVDTQKDTVEVGPGNLKMSFSIA 480
             K+ L F  SVP LG+++Y IS      +G S  ++   +    VEVG GNLK+ +S  
Sbjct: 552 TAKHVLAFTASVPPLGFSSYVISDTGRTARGLSASYVTSGSMNQNVEVGQGNLKLRYSEE 611

Query: 481 SGQLKRVYNSKTGVDVPIQQSYLWYASATGEPNIVSRSVSLMF------PL-SLSYVAVE 518
             ++ R  ++K    V  +QSY +Y  + G       S + +F      P+ S     + 
Sbjct: 612 GVKITRHLSTKN--QVTAEQSYAYYIGSNGTDKDPQASGAYVFRPDGVLPIKSKEEAQLT 671

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023545016.12.2e-25872.85alpha-mannosidase [Cucurbita pepo subsp. pepo][more]
XP_038883639.18.3e-25872.93alpha-mannosidase [Benincasa hispida][more]
XP_022950420.12.4e-25772.85alpha-mannosidase [Cucurbita moschata][more]
XP_022977427.15.4e-25772.70alpha-mannosidase [Cucurbita maxima][more]
XP_004135022.12.7e-25672.61alpha-mannosidase [Cucumis sativus] >KGN48931.1 hypothetical protein Csa_003885 ... [more]
Match NameE-valueIdentityDescription
Q9FKW94.4e-18552.07Probable alpha-mannosidase At5g66150 OS=Arabidopsis thaliana OX=3702 GN=At5g6615... [more]
C0HJB32.2e-18453.25Alpha-mannosidase OS=Canavalia ensiformis OX=3823 PE=1 SV=1[more]
Q8LPJ31.5e-16448.66Probable alpha-mannosidase At5g13980 OS=Arabidopsis thaliana OX=3702 GN=At5g1398... [more]
P940784.6e-15847.86Alpha-mannosidase At3g26720 OS=Arabidopsis thaliana OX=3702 GN=At3g26720 PE=1 SV... [more]
P340982.0e-8430.92Lysosomal alpha-mannosidase OS=Dictyostelium discoideum OX=44689 GN=manA PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1GFN91.2e-25772.85Alpha-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111453525 PE=3 SV=1[more]
A0A6J1IIE42.6e-25772.70Alpha-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111477773 PE=3 SV=1[more]
A0A0A0KMH91.3e-25672.61Alpha-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G507060 PE=3 SV=1[more]
A0A5D3CQ321.4e-25571.99Alpha-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00... [more]
A0A1S3B2P61.4e-25571.99Alpha-mannosidase OS=Cucumis melo OX=3656 GN=LOC103485145 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G66150.13.1e-18652.07Glycosyl hydrolase family 38 protein [more]
AT5G13980.11.0e-16548.66Glycosyl hydrolase family 38 protein [more]
AT5G13980.21.0e-16548.66Glycosyl hydrolase family 38 protein [more]
AT5G13980.31.0e-16548.66Glycosyl hydrolase family 38 protein [more]
AT3G26720.13.2e-15947.86Glycosyl hydrolase family 38 protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015341Glycoside hydrolase family 38, central domainSMARTSM00872Alpha_mann_mid_2coord: 186..260
e-value: 4.7E-21
score: 85.9
IPR015341Glycoside hydrolase family 38, central domainPFAMPF09261Alpha-mann_midcoord: 186..271
e-value: 8.4E-17
score: 61.1
IPR000602Glycoside hydrolase family 38, N-terminal domainPFAMPF01074Glyco_hydro_38Ncoord: 1..143
e-value: 5.3E-34
score: 117.9
IPR027291Glycoside hydrolase 38, N-terminal domain superfamilyGENE3D3.20.110.10Glycoside hydrolase 38, N terminal domaincoord: 1..184
e-value: 5.6E-48
score: 165.8
NoneNo IPR availableGENE3D2.70.98.30coord: 375..517
e-value: 5.3E-30
score: 106.2
NoneNo IPR availablePANTHERPTHR11607:SF61ALPHA-MANNOSIDASEcoord: 1..145
coord: 265..517
NoneNo IPR availablePANTHERPTHR11607:SF61ALPHA-MANNOSIDASEcoord: 144..266
NoneNo IPR availablePANTHERPTHR11607ALPHA-MANNOSIDASEcoord: 265..517
NoneNo IPR availablePANTHERPTHR11607ALPHA-MANNOSIDASEcoord: 1..145
coord: 144..266
IPR011682Glycosyl hydrolase family 38, C-terminalPFAMPF07748Glyco_hydro_38Ccoord: 379..517
e-value: 5.4E-13
score: 49.5
IPR037094Glycoside hydrolase family 38, central domain superfamilyGENE3D1.20.1270.50Glycoside hydrolase family 38, central domaincoord: 185..274
e-value: 3.6E-24
score: 86.9
IPR011330Glycoside hydrolase/deacetylase, beta/alpha-barrelSUPERFAMILY88713Glycoside hydrolase/deacetylasecoord: 1..182
IPR028995Glycoside hydrolase families 57/38, central domain superfamilySUPERFAMILY88688Families 57/38 glycoside transferase middle domaincoord: 185..273
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 262..508

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012064.1Spg012064.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006013 mannose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0004559 alpha-mannosidase activity
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0003824 catalytic activity