Spg012050 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg012050
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	glucan endo-1,3-beta-glucosidase 12-like isoform X8
Location	scaffold1: 1943728 .. 1944942 (+)
RNA-Seq Expression	Spg012050
Synteny	Spg012050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTACCTGCATTCACCTTCATTCTGCATTACTTTCCTTGTTTGTCTTCCTCAATCTAGGTAAATCAGCATTCACTCAATGCTTTTTTTCTGCACAATGATATTATGTTAATCTTCTAACAATAAATGCATCATGTAACTGTTATTACTGCTAATCTGCTACTAATGTCTGGATTATCTTTCTGCACCTCATCTATTGGTTGATGTTGATGTATCAGAAGGAAAACCAAACGTAATCTTTATCTTCACCCTGTATTCTAGGATAATCATAATGTGAAGGGGTTCCACTTTTTTATGTTTCTATGAAGAGAATTATGAACTGACCTGACTTTTTGTGCCTTCAGGTTCAAGTATTGCACAGACACCGTTTTTTGAAGCAAACCGAGAAAACTCTGCACTGCAAAGTCAAGAAGATCGATTATCCTACTTTTCAACTCCAATATCTAACACTCAACTGGACACAATCCCCATTGTGAATCCAACCACCCCAGGCGGCGCAACTCCGACACCGATTGTGAACCCAACATCGCCGCCAGCACAACAAATCCCAACCGGACCGACCATAACGCCGACAACCCCAACAACCACAGGCGGTGGAAGTTGGTGCATAGCAAGCCCAAATGCATCCCCAACCGCTTTGCAGGTAGCTCTTGACTATGCTTGCGGCTATGGAGGCGCAGATTGCTCTGCAATTCAAACGGGCGGGAGTTGCTTTGAGCCCAACACCATGCGAGACCATGCTTCTTATGCCTTCAACGACTACTACCAGAAGAATCCAGCACCCACAAGCTGCGTGTTTGGAGGAACAGCGCAGCTCTCCAGTACAGACCCCAGTAAGAAAGATCATCCATCACTTCAAAGTTTTCTAAATTCAAAACCATAACAAAAACACAGGCAATAATCCATATCCAATACTGAAAAGAGAATGAGAAACATTACAACAATAAGTATTTGGTCATGATTGTTGCTAGCTGTTTCATTTCATTTTGAACAAAAATTCGAAAATTTGTTTCCTATAATGCTTGCTTAAAGTGCTTAAATTAAAAAAATGAATACTAAACACATTCCTCTTTCAATCTGTTTCCTATCTAATAAAGTATTTACTATACGAAACAAATTTCCATCCTAATAAACCAAATATTCATTACTCTTTCTTGACGATTGCAGGCAATGGAAGTTGTCGCTACGCAGCACCAAGATCAACGCCAAGGTAA

mRNA sequence

ATGGGTACCTGCATTCACCTTCATTCTGCATTACTTTCCTTGTTTGTCTTCCTCAATCTAGGTTCAAGTATTGCACAGACACCGTTTTTTGAAGCAAACCGAGAAAACTCTGCACTGCAAAGTCAAGAAGATCGATTATCCTACTTTTCAACTCCAATATCTAACACTCAACTGGACACAATCCCCATTGTGAATCCAACCACCCCAGGCGGCGCAACTCCGACACCGATTGTGAACCCAACATCGCCGCCAGCACAACAAATCCCAACCGGACCGACCATAACGCCGACAACCCCAACAACCACAGGCGGTGGAAGTTGGTGCATAGCAAGCCCAAATGCATCCCCAACCGCTTTGCAGGTAGCTCTTGACTATGCTTGCGGCTATGGAGGCGCAGATTGCTCTGCAATTCAAACGGGCGGGAGTTGCTTTGAGCCCAACACCATGCGAGACCATGCTTCTTATGCCTTCAACGACTACTACCAGAAGAATCCAGCACCCACAAGCTGCGTGTTTGGAGGAACAGCGCAGCTCTCCAGTACAGACCCCAGCAATGGAAGTTGTCGCTACGCAGCACCAAGATCAACGCCAAGGTAA

Coding sequence (CDS)

ATGGGTACCTGCATTCACCTTCATTCTGCATTACTTTCCTTGTTTGTCTTCCTCAATCTAGGTTCAAGTATTGCACAGACACCGTTTTTTGAAGCAAACCGAGAAAACTCTGCACTGCAAAGTCAAGAAGATCGATTATCCTACTTTTCAACTCCAATATCTAACACTCAACTGGACACAATCCCCATTGTGAATCCAACCACCCCAGGCGGCGCAACTCCGACACCGATTGTGAACCCAACATCGCCGCCAGCACAACAAATCCCAACCGGACCGACCATAACGCCGACAACCCCAACAACCACAGGCGGTGGAAGTTGGTGCATAGCAAGCCCAAATGCATCCCCAACCGCTTTGCAGGTAGCTCTTGACTATGCTTGCGGCTATGGAGGCGCAGATTGCTCTGCAATTCAAACGGGCGGGAGTTGCTTTGAGCCCAACACCATGCGAGACCATGCTTCTTATGCCTTCAACGACTACTACCAGAAGAATCCAGCACCCACAAGCTGCGTGTTTGGAGGAACAGCGCAGCTCTCCAGTACAGACCCCAGCAATGGAAGTTGTCGCTACGCAGCACCAAGATCAACGCCAAGGTAA

Protein sequence

MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDTIPIVNPTTPGGATPTPIVNPTSPPAQQIPTGPTITPTTPTTTGGGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQLSSTDPSNGSCRYAAPRSTPR

Homology

[Close]

BLAST of Spg012050 vs. NCBI nr
Match: XP_022142225.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like [Momordica charantia])

HSP 1 Score: 341.3 bits (874), Expect = 5.7e-90
Identity = 172/200 (86.00%), Postives = 184/200 (92.00%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTCIHLH A LSLF+FLNLGSSIA+TPF  AN+ENS LQ+QED++ +FSTP+S+TQLDT
Sbjct: 1   MGTCIHLHFASLSLFIFLNLGSSIAETPFLRANQENSVLQNQEDQIPFFSTPLSSTQLDT 60

Query: 61  IPIVNPTTPGGATP--TPIVNPTSPPAQQIPTGPTITPTTP-TTTGGGSWCIASPNASPT 120
           IPIVNPT PGG TP  TPI NPTSPPAQ I TGPTITPTTP TTTGGGSWCIASP ASPT
Sbjct: 61  IPIVNPTIPGGTTPTTTPITNPTSPPAQPISTGPTITPTTPVTTTGGGSWCIASPAASPT 120

Query: 121 ALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQ 180
           ALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQ
Sbjct: 121 ALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQ 180

Query: 181 LSSTDPSNGSCRYAAPRSTP 198
           +++TDPS GSCRYAAPRSTP
Sbjct: 181 ITNTDPSTGSCRYAAPRSTP 200

[Close]

BLAST of Spg012050 vs. NCBI nr
Match: XP_038882928.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Benincasa hispida])

HSP 1 Score: 339.7 bits (870), Expect = 1.7e-89
Identity = 171/199 (85.93%), Postives = 182/199 (91.46%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTCIHLH ALLS  VFL+LGSSIAQ PFFEAN+E+S LQ+QE+++ YFST  S+ QLDT
Sbjct: 1   MGTCIHLHFALLSFLVFLSLGSSIAQRPFFEANQEDSVLQNQEEQIPYFSTSTSSNQLDT 60

Query: 61  IPIVNPTTPGGATP--TPIVNPTSPPAQQIPTGPTITPTTPTTTGGGSWCIASPNASPTA 120
           IPIVNPTTPGG TP  TPIVNP SPPAQ IPTGP ITPTTPTTTGGGSWCIASPNASPTA
Sbjct: 61  IPIVNPTTPGGTTPTQTPIVNPPSPPAQTIPTGPIITPTTPTTTGGGSWCIASPNASPTA 120

Query: 121 LQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQL 180
           LQVALDYACGYGGADCSAIQ+GGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQL
Sbjct: 121 LQVALDYACGYGGADCSAIQSGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQL 180

Query: 181 SSTDPSNGSCRYAAPRSTP 198
           +STDPSNG+C YAA RSTP
Sbjct: 181 TSTDPSNGNCHYAASRSTP 199

[Close]

BLAST of Spg012050 vs. NCBI nr
Match: KAG7034687.1 (Glucan endo-1,3-beta-glucosidase 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 326.6 bits (836), Expect = 1.4e-85
Identity = 169/207 (81.64%), Postives = 186/207 (89.86%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTC+ LH+ALLSL +FL+LGSSIA+TP+FEAN+E+  LQ+QE+++ YFST ISNTQLDT
Sbjct: 1   MGTCVRLHTALLSLLIFLSLGSSIARTPYFEANQEDYELQNQEEQIPYFSTSISNTQLDT 60

Query: 61  IPIVNPTTP-GGATP--TPIVNPTSPPAQQIPTG-------PTITPTTPTTTGGGSWCIA 120
           IPIVNPTTP GG TP  TPIV+PTSPPAQ IPTG       PTITPTTPTTTGGGSWCIA
Sbjct: 61  IPIVNPTTPVGGTTPTQTPIVDPTSPPAQTIPTGPTITPTTPTITPTTPTTTGGGSWCIA 120

Query: 121 SPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSC 180
           S +ASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFN YYQKNPAPTSC
Sbjct: 121 SSSASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNQYYQKNPAPTSC 180

Query: 181 VFGGTAQLSSTDPSNGSCRYAAPRSTP 198
           VFGGTAQL+STDPS+G+CRYAA RSTP
Sbjct: 181 VFGGTAQLTSTDPSSGNCRYAASRSTP 207

[Close]

BLAST of Spg012050 vs. NCBI nr
Match: XP_022978734.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 325.5 bits (833), Expect = 3.2e-85
Identity = 168/214 (78.50%), Postives = 185/214 (86.45%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTC+ LH+ALLSL +FL+LGSSIA+TP+FEAN+E+  LQ+QE+++ YFST ISNTQLDT
Sbjct: 1   MGTCVRLHTALLSLLIFLSLGSSIARTPYFEANQEDYVLQNQEEQIPYFSTSISNTQLDT 60

Query: 61  IPIVNPTTPGGA---TPTPIVNPTSPPAQQIPTG--------------PTITPTTPTTTG 120
           IPIVNPTTPGG    T TPIV+PTSPPAQ IPTG              PTITPTTPTTTG
Sbjct: 61  IPIVNPTTPGGGTTPTQTPIVDPTSPPAQTIPTGPTITPTTPTITPTTPTITPTTPTTTG 120

Query: 121 GGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQK 180
           GGSWCIAS +ASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFN YYQK
Sbjct: 121 GGSWCIASSSASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNQYYQK 180

Query: 181 NPAPTSCVFGGTAQLSSTDPSNGSCRYAAPRSTP 198
           NPAPTSCVFGGTAQL+STDPS+G+CRYAA RSTP
Sbjct: 181 NPAPTSCVFGGTAQLTSTDPSSGNCRYAASRSTP 214

[Close]

BLAST of Spg012050 vs. NCBI nr
Match: XP_022925629.1 (glucan endo-1,3-beta-glucosidase 12-like isoform X11 [Cucurbita moschata])

HSP 1 Score: 324.3 bits (830), Expect = 7.2e-85
Identity = 169/214 (78.97%), Postives = 186/214 (86.92%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTC+ LH+ALLSL +FL+LGSSIA+TP+FEAN+E+  LQ+QE+++ YFST ISNTQLDT
Sbjct: 1   MGTCVRLHTALLSLLIFLSLGSSIARTPYFEANQEDYVLQNQEEQIPYFSTSISNTQLDT 60

Query: 61  IPIVNPTTP-GGATP--TPIVNPTSPPAQQIPTG--------------PTITPTTPTTTG 120
           IPIVNPTTP GG TP  TPIV+PTSPPAQ IPTG              PTITPTTPTTTG
Sbjct: 61  IPIVNPTTPVGGTTPTQTPIVDPTSPPAQTIPTGPTITPTTPTITPTTPTITPTTPTTTG 120

Query: 121 GGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQK 180
           GGSWCIAS +ASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFN YYQK
Sbjct: 121 GGSWCIASSSASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNQYYQK 180

Query: 181 NPAPTSCVFGGTAQLSSTDPSNGSCRYAAPRSTP 198
           NPAPTSCVFGGTAQL+STDPS+G+CRYAA RSTP
Sbjct: 181 NPAPTSCVFGGTAQLTSTDPSSGNCRYAASRSTP 214

[Close]

BLAST of Spg012050 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 7.1e-19
Identity = 51/120 (42.50%), Postives = 71/120 (59.17%), Query Frame = 0


Query: 74  PTPIVNPTSPPAQQIPTGPTITPTTPTTTGGGSWCIASPNASPTALQVALDYACGYGGAD 133
           PTP  NP+  P+ +           P  +GGG WC+A   A+ T LQ  ++YACG+   D
Sbjct: 355 PTPSPNPSPNPSPK-----------PAPSGGGKWCVAKDGANGTDLQNNINYACGF--VD 414

Query: 134 CSAIQTGGSCFEPNTMRDHASYAFNDYYQKN-PAPTSCVFGGTAQLSSTDPSNGSCRYAA 193
           C  IQ+GG+CF PN+++ HASY  N YYQ N     +C F GT  ++S+DPS G C+Y +
Sbjct: 415 CKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCKYVS 461

[Close]

BLAST of Spg012050 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.6e-18
Identity = 49/112 (43.75%), Postives = 61/112 (54.46%), Query Frame = 0


Query: 80  PTSPPAQQIPTGPTITPTTPTTTGGGSWCIASPNASPTALQVALDYACGYGGADCSAIQT 139
           P   P   +PT  +  P  P T G   WC+    A+   LQ  +DY C   G DC  IQ 
Sbjct: 11  PVPTPTPSVPTSSSPVP-KPPTQGNKKWCVPKAEATDAQLQSNIDYVCSQSGMDCGPIQA 70

Query: 140 GGSCFEPNTMRDHASYAFNDYYQ-KNPAPTSCVFGGTAQLSSTDPSNGSCRY 191
            G+CF PNT+R HASYA N +YQ K      C F GT  ++S+DPSNGSC +
Sbjct: 71  NGACFNPNTVRAHASYAMNSWYQSKGRNDFDCDFSGTGAITSSDPSNGSCSF 121

[Close]

BLAST of Spg012050 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.1e-18
Identity = 59/136 (43.38%), Postives = 73/136 (53.68%), Query Frame = 0


Query: 68  TPGGATPTPIVNPTSPPAQQIPTGPTITPTTPTTTGGG------SWCIASPNASPTALQV 127
           T    TP    N T+  +    + P I   +  T GGG       WCIAS  AS T LQ 
Sbjct: 346 TGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQT 405

Query: 128 ALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPT-SCVFGGTAQLSS 187
           ALD+ACG G  DCSA+Q    CFEP+T+  HASYAFN YYQ++ A +  C F G +    
Sbjct: 406 ALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVD 465

Query: 188 TDPSNGSCRYAAPRST 197
            DPS G+C Y    +T
Sbjct: 466 KDPSYGNCLYMIAPAT 481

[Close]

BLAST of Spg012050 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 92.0 bits (227), Expect = 7.9e-18
Identity = 45/88 (51.14%), Postives = 59/88 (67.05%), Query Frame = 0


Query: 102 TGGGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYY 161
           T   ++CIA        LQ ALD+ACG G ++CS IQ G SC++PN ++ HAS+AFN YY
Sbjct: 376 TTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYY 435

Query: 162 QK-NPAPTSCVFGGTAQLSSTDPSNGSC 189
           QK   A  SC F G A +++TDPS+GSC
Sbjct: 436 QKEGRASGSCDFKGVAMITTTDPSHGSC 463

[Close]

BLAST of Spg012050 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 3.0e-17
Identity = 47/97 (48.45%), Postives = 63/97 (64.95%), Query Frame = 0


Query: 95  TPTTPTTTGGGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHAS 154
           TP+   T+ G  WC+    A+   LQ +LD+ACG+ G DC AIQ GG+CFEPN +  HA+
Sbjct: 354 TPSGKVTSSG--WCVPKKGATNEELQASLDWACGH-GIDCGAIQPGGACFEPNNVVSHAA 413

Query: 155 YAFNDYYQKNP-APTSCVFGGTAQLSSTDPSNGSCRY 191
           YA N Y+QK+P  PT C F  TA ++S +PS  +C Y
Sbjct: 414 YAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVY 447

[Close]

BLAST of Spg012050 vs. ExPASy TrEMBL
Match: A0A6J1CKB4 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Momordica charantia OX=3673 GN=LOC111012393 PE=4 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 2.8e-90
Identity = 172/200 (86.00%), Postives = 184/200 (92.00%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTCIHLH A LSLF+FLNLGSSIA+TPF  AN+ENS LQ+QED++ +FSTP+S+TQLDT
Sbjct: 1   MGTCIHLHFASLSLFIFLNLGSSIAETPFLRANQENSVLQNQEDQIPFFSTPLSSTQLDT 60

Query: 61  IPIVNPTTPGGATP--TPIVNPTSPPAQQIPTGPTITPTTP-TTTGGGSWCIASPNASPT 120
           IPIVNPT PGG TP  TPI NPTSPPAQ I TGPTITPTTP TTTGGGSWCIASP ASPT
Sbjct: 61  IPIVNPTIPGGTTPTTTPITNPTSPPAQPISTGPTITPTTPVTTTGGGSWCIASPAASPT 120

Query: 121 ALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQ 180
           ALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQ
Sbjct: 121 ALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGTAQ 180

Query: 181 LSSTDPSNGSCRYAAPRSTP 198
           +++TDPS GSCRYAAPRSTP
Sbjct: 181 ITNTDPSTGSCRYAAPRSTP 200

[Close]

BLAST of Spg012050 vs. ExPASy TrEMBL
Match: A0A6J1IUV4 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478603 PE=4 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.6e-85
Identity = 168/214 (78.50%), Postives = 185/214 (86.45%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTC+ LH+ALLSL +FL+LGSSIA+TP+FEAN+E+  LQ+QE+++ YFST ISNTQLDT
Sbjct: 1   MGTCVRLHTALLSLLIFLSLGSSIARTPYFEANQEDYVLQNQEEQIPYFSTSISNTQLDT 60

Query: 61  IPIVNPTTPGGA---TPTPIVNPTSPPAQQIPTG--------------PTITPTTPTTTG 120
           IPIVNPTTPGG    T TPIV+PTSPPAQ IPTG              PTITPTTPTTTG
Sbjct: 61  IPIVNPTTPGGGTTPTQTPIVDPTSPPAQTIPTGPTITPTTPTITPTTPTITPTTPTTTG 120

Query: 121 GGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQK 180
           GGSWCIAS +ASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFN YYQK
Sbjct: 121 GGSWCIASSSASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNQYYQK 180

Query: 181 NPAPTSCVFGGTAQLSSTDPSNGSCRYAAPRSTP 198
           NPAPTSCVFGGTAQL+STDPS+G+CRYAA RSTP
Sbjct: 181 NPAPTSCVFGGTAQLTSTDPSSGNCRYAASRSTP 214

[Close]

BLAST of Spg012050 vs. ExPASy TrEMBL
Match: A0A6J1EFR2 (glucan endo-1,3-beta-glucosidase 12-like isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111432992 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.5e-85
Identity = 169/214 (78.97%), Postives = 186/214 (86.92%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTC+ LH+ALLSL +FL+LGSSIA+TP+FEAN+E+  LQ+QE+++ YFST ISNTQLDT
Sbjct: 1   MGTCVRLHTALLSLLIFLSLGSSIARTPYFEANQEDYVLQNQEEQIPYFSTSISNTQLDT 60

Query: 61  IPIVNPTTP-GGATP--TPIVNPTSPPAQQIPTG--------------PTITPTTPTTTG 120
           IPIVNPTTP GG TP  TPIV+PTSPPAQ IPTG              PTITPTTPTTTG
Sbjct: 61  IPIVNPTTPVGGTTPTQTPIVDPTSPPAQTIPTGPTITPTTPTITPTTPTITPTTPTTTG 120

Query: 121 GGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQK 180
           GGSWCIAS +ASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFN YYQK
Sbjct: 121 GGSWCIASSSASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNQYYQK 180

Query: 181 NPAPTSCVFGGTAQLSSTDPSNGSCRYAAPRSTP 198
           NPAPTSCVFGGTAQL+STDPS+G+CRYAA RSTP
Sbjct: 181 NPAPTSCVFGGTAQLTSTDPSSGNCRYAASRSTP 214

[Close]

BLAST of Spg012050 vs. ExPASy TrEMBL
Match: A0A6J1ECQ8 (glucan endo-1,3-beta-glucosidase 12-like isoform X11 OS=Cucurbita moschata OX=3662 GN=LOC111432992 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.5e-85
Identity = 169/214 (78.97%), Postives = 186/214 (86.92%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTC+ LH+ALLSL +FL+LGSSIA+TP+FEAN+E+  LQ+QE+++ YFST ISNTQLDT
Sbjct: 1   MGTCVRLHTALLSLLIFLSLGSSIARTPYFEANQEDYVLQNQEEQIPYFSTSISNTQLDT 60

Query: 61  IPIVNPTTP-GGATP--TPIVNPTSPPAQQIPTG--------------PTITPTTPTTTG 120
           IPIVNPTTP GG TP  TPIV+PTSPPAQ IPTG              PTITPTTPTTTG
Sbjct: 61  IPIVNPTTPVGGTTPTQTPIVDPTSPPAQTIPTGPTITPTTPTITPTTPTITPTTPTTTG 120

Query: 121 GGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQK 180
           GGSWCIAS +ASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFN YYQK
Sbjct: 121 GGSWCIASSSASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNQYYQK 180

Query: 181 NPAPTSCVFGGTAQLSSTDPSNGSCRYAAPRSTP 198
           NPAPTSCVFGGTAQL+STDPS+G+CRYAA RSTP
Sbjct: 181 NPAPTSCVFGGTAQLTSTDPSSGNCRYAASRSTP 214

[Close]

BLAST of Spg012050 vs. ExPASy TrEMBL
Match: A0A6J1EFR7 (glucan endo-1,3-beta-glucosidase 12-like isoform X10 OS=Cucurbita moschata OX=3662 GN=LOC111432992 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.5e-85
Identity = 169/214 (78.97%), Postives = 186/214 (86.92%), Query Frame = 0


Query: 1   MGTCIHLHSALLSLFVFLNLGSSIAQTPFFEANRENSALQSQEDRLSYFSTPISNTQLDT 60
           MGTC+ LH+ALLSL +FL+LGSSIA+TP+FEAN+E+  LQ+QE+++ YFST ISNTQLDT
Sbjct: 1   MGTCVRLHTALLSLLIFLSLGSSIARTPYFEANQEDYVLQNQEEQIPYFSTSISNTQLDT 60

Query: 61  IPIVNPTTP-GGATP--TPIVNPTSPPAQQIPTG--------------PTITPTTPTTTG 120
           IPIVNPTTP GG TP  TPIV+PTSPPAQ IPTG              PTITPTTPTTTG
Sbjct: 61  IPIVNPTTPVGGTTPTQTPIVDPTSPPAQTIPTGPTITPTTPTITPTTPTITPTTPTTTG 120

Query: 121 GGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQK 180
           GGSWCIAS +ASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFN YYQK
Sbjct: 121 GGSWCIASSSASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNQYYQK 180

Query: 181 NPAPTSCVFGGTAQLSSTDPSNGSCRYAAPRSTP 198
           NPAPTSCVFGGTAQL+STDPS+G+CRYAA RSTP
Sbjct: 181 NPAPTSCVFGGTAQLTSTDPSSGNCRYAASRSTP 214

[Close]

BLAST of Spg012050 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 1.5e-35
Identity = 90/201 (44.78%), Postives = 105/201 (52.24%), Query Frame = 0


Query: 61  IPIVNPTTPGGATPTPIVNPTSPPA-----QQIPTG--PTITPTTPT--------TT--- 120
           IP+VNPT PGG+T TP +   SPP+        PTG  P +  TTPT        TT   
Sbjct: 37  IPVVNPTAPGGSTTTPTITQPSPPSLTFPGPTTPTGGYPPLDGTTPTGGYPPLYGTTPPG 96

Query: 121 -----------------------------------------------GGGSWCIASPNAS 180
                                                          G G WCIA  NAS
Sbjct: 97  GGDVGGGGGGYGGGTPGGGGGGGGDTGAGAGGGGYGGGGDTGAGGGVGSGQWCIAKANAS 156

Query: 181 PTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAPTSCVFGGT 197
           PT+LQVALDYACGYGGADC  IQ G +C+EPNT+RDHAS+AFN YYQK+P   SC FGG 
Sbjct: 157 PTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQKHPGSDSCNFGGA 216

[Close]

BLAST of Spg012050 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 127.9 bits (320), Expect = 9.2e-30
Identity = 73/143 (51.05%), Postives = 89/143 (62.24%), Query Frame = 0


Query: 52  PISNTQLDTIPI-VNPTTPGGATPTPIVNPTSPPAQQIPTGPTITPTTP---TTTGGGSW 111
           PI   +  T P+  NPTT    TPT +V  +   A  + T P   P++P      G  SW
Sbjct: 27  PIEEEKDITTPLATNPTT----TPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGDQSW 86

Query: 112 CIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAP 171
           C+A  N +  ALQ ALDYACG GGADCS IQ GG+C+ PN++R HAS+AFN YYQKNP P
Sbjct: 87  CVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPIP 146

Query: 172 TSCVFGGTAQLSSTDPSNGSCRY 191
           +SC F GTA   S DPS GSC +
Sbjct: 147 SSCNFDGTAITISADPSLGSCHF 165

[Close]

BLAST of Spg012050 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.5e-27
Identity = 70/137 (51.09%), Postives = 85/137 (62.04%), Query Frame = 0


Query: 52  PISNTQLDTIPI-VNPTTPGGATPTPIVNPTSPPAQQIPTGPTITPTTP---TTTGGGSW 111
           PI   +  T P+  NPTT    TPT +V  +   A  + T P   P++P      G  SW
Sbjct: 27  PIEEEKDITTPLATNPTT----TPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGDQSW 86

Query: 112 CIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQKNPAP 171
           C+A  N +  ALQ ALDYACG GGADCS IQ GG+C+ PN++R HAS+AFN YYQKNP P
Sbjct: 87  CVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPIP 146

Query: 172 TSCVFGGTAQLSSTDPS 185
           +SC F GTA   S DPS
Sbjct: 147 SSCNFDGTAITISADPS 159

[Close]

BLAST of Spg012050 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 4.3e-27
Identity = 75/165 (45.45%), Postives = 93/165 (56.36%), Query Frame = 0


Query: 60  TIPIVNPTT---PGGATPTPIV------------NP-TSPPAQQIPTGPTITPTTPT--- 119
           T+PIV P T   P   TP P++            NP T+P  Q  PT P+ T   P    
Sbjct: 62  TVPIVPPVTTIPPPTLTPPPVITIPPPTLTPPVTNPVTNPVTQYPPTQPSGTVPVPVPVV 121

Query: 120 ---------TTGGGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRD 179
                    +  G SWC+A P AS  +LQ ALDYACG   ADCS +Q GG+C+ P +++ 
Sbjct: 122 APPVVSNSPSVSGQSWCVAKPGASQVSLQQALDYACGI--ADCSQLQQGGNCYSPISLQS 181

Query: 180 HASYAFNDYYQKNPAPTSCVFGGTAQLSSTDPSNGSCRYAAPRST 197
           HAS+AFN YYQKNP+P SC FGG A L +T+PS GSC Y    ST
Sbjct: 182 HASFAFNSYYQKNPSPQSCDFGGAASLVNTNPSTGSCIYQTGSST 224

[Close]

BLAST of Spg012050 vs. TAIR 10
Match: AT4G05430.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-20
Identity = 47/93 (50.54%), Postives = 60/93 (64.52%), Query Frame = 0


Query: 104 GGSWCIASPNASPTALQVALDYACGYGGADCSAIQTGGSCFEPNTMRDHASYAFNDYYQK 163
           G +WC+A P A+   LQ ALD+ACG G  DCS I+  G C+EP+T+  HAS+AFN YYQ 
Sbjct: 20  GTTWCVARPGATQAELQRALDWACGIGRVDCSVIERHGDCYEPDTIVSHASFAFNAYYQT 79

Query: 164 N-PAPTSCVFGGTAQLSSTDPSNGSCRYAAPRS 196
           N     +C FGGTA  +  +PS G C Y A +S
Sbjct: 80  NGNNRIACYFGGTATFTKINPSYGKCSYDASKS 112

The following BLAST results are available for this feature:

BLAST of Spg012050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142225.1	5.7e-90	86.00	PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like [Momordica charantia]	[more]
XP_038882928.1	1.7e-89	85.93	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like [Benincasa hispida]	[more]
KAG7034687.1	1.4e-85	81.64	Glucan endo-1,3-beta-glucosidase 12 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022978734.1	3.2e-85	78.50	PLASMODESMATA CALLOSE-BINDING PROTEIN 2-like isoform X1 [Cucurbita maxima]	[more]
XP_022925629.1	7.2e-85	78.97	glucan endo-1,3-beta-glucosidase 12-like isoform X11 [Cucurbita moschata]	[more]

BLAST of Spg012050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52409	7.1e-19	42.50	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q84V39	1.6e-18	43.75	Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q8VYE5	2.1e-18	43.38	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
O65399	7.9e-18	51.14	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q9M069	3.0e-17	48.45	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]

BLAST of Spg012050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKB4	2.8e-90	86.00	PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1IUV4	1.6e-85	78.50	PLASMODESMATA CALLOSE-BINDING PROTEIN 2-like isoform X1 OS=Cucurbita maxima OX=3...	[more]
A0A6J1EFR2	3.5e-85	78.97	glucan endo-1,3-beta-glucosidase 12-like isoform X5 OS=Cucurbita moschata OX=366...	[more]
A0A6J1ECQ8	3.5e-85	78.97	glucan endo-1,3-beta-glucosidase 12-like isoform X11 OS=Cucurbita moschata OX=36...	[more]
A0A6J1EFR7	3.5e-85	78.97	glucan endo-1,3-beta-glucosidase 12-like isoform X10 OS=Cucurbita moschata OX=36...	[more]

BLAST of Spg012050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29380.1	1.5e-35	44.78	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.1	9.2e-30	51.05	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.2	1.5e-27	51.09	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G09460.1	4.3e-27	45.45	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G05430.1	1.3e-20	50.54	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 106..190 e-value: 1.6E-47 score: 173.9
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 107..176 e-value: 2.9E-21 score: 75.9
None	No IPR available	GENE3D	1.20.58.1040		coord: 104..195 e-value: 3.4E-11 score: 45.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..103
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 178..198
None	No IPR available	PANTHER	PTHR31044:SF79	BETA-1,3 GLUCANASE	coord: 10..191
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 10..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg012050.1	Spg012050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds