Spg012011 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg012011
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	gibberellin 2-beta-dioxygenase-like
Location	scaffold1: 1693878 .. 1695284 (-)
RNA-Seq Expression	Spg012011
Synteny	Spg012011

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCAATTCCAATTCCAATTCCAATTCCAATTCCAATTCAAATTCATCTATAATCCGCCATGGTTGTTCTGTCACAGCCAGCATTGGGTTTAGACCAATATTCTCTTCTTCGAACAACATGCAAACCCACCGCCTCCTTCACCGAAATCCCTGAAATCGACCTCTCAGACCCCAACGCCCAGTCCCACATCGTCGAGGCCTGTGAAGAATTCGGCTTCTTTAAGCTCGTAAACCATCGAGTTCCGATTGAATTGATGACCAAACTCGAAGAAGAAGCCCTCTGTTTTTTCAAACTTTCCCAGTCTGAGAAAGATAAAGCTCGCCCTCCTGATCCTTTAGGCTATGGCTGCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATTGAATACCTCCTCCTCACCGCCAATCCTCTCCCCATTTTCTCCCAAAACTCCGAATCTTTCCTGTAAGTTGCAATACAAACAGAGCACTCTGTTTTTTTTTTTTTAAGAATCCACTTAGATTTTGAAAAAAGTATTATTTATAAAGAATAAAATCTTTCCTTCACGTCATTTTCTCGTGTTACTGATTTTGGGCAAATTTTCCTGTAGCGCCGCCGCGAAGGAGTACGTCACGGCGGTGAAGAAGCTAAGCTGCGAAGTGGCGGAATTGATAGCAGAGGGATTGAAAATCGAGCCACGAAACGCAATAAGCAAGCTTCTAAACGACGAGAAAAGCGACTGTTGTTTCAGAGTGAACCACTACCCGCCATGTCCGGACCTCCAAGCTCTAGGCGGAAGTGGAAGGAACATGATCGGATTCGGGGAACACACAGACCCACAAATCATATCGCTGCTAAGATCAAACAACTCGACCGGCCTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCGGACGCGGCGGCGATCTTCGTGAACGTCGGCGACTCGCTGCAGGTGATGAGCAACGGCAGATTCAAAAGCGTGAAGCACAAAGTGGTGGCGGATTCCAACAGAGAGAGGGTTTCGATGATTTACTTCGGAGGGCCGCCGTTGAGTGAAAAGATTGCGCCATTGCCGGAGGTTTTGGGCGACGGAGAAGAAAGCTTGTACAAGGAATTCACTTGGTGGGAATACAAAACGGCGGCGTATAAGACCAGATTGGCTGATTACCGCCTCGGTCCCTTTGAGAAGAATCAAAATTCTTAGACGTTTTCATTTCCTTCCATAATTGGAAAATCATAAAATCTCTTTTCTACCAAGAAATTATTGGAGGAGTCTCATCTATCCGTTTTTTTTTTTTTTTTTTATTATTATTATTGAAAAAAAAAATATTTGTGTTTTGTATTTTTTTTTAATCTTTTGAACTGTTACTTGCTGATCAAGGGTTGTAAAGAAAATAGTAAACTGTTATGGG

mRNA sequence

ATGGTTGTTCTGTCACAGCCAGCATTGGGTTTAGACCAATATTCTCTTCTTCGAACAACATGCAAACCCACCGCCTCCTTCACCGAAATCCCTGAAATCGACCTCTCAGACCCCAACGCCCAGTCCCACATCGTCGAGGCCTGTGAAGAATTCGGCTTCTTTAAGCTCGTAAACCATCGAGTTCCGATTGAATTGATGACCAAACTCGAAGAAGAAGCCCTCTGTTTTTTCAAACTTTCCCAGTCTGAGAAAGATAAAGCTCGCCCTCCTGATCCTTTAGGCTATGGCTGCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATTGAATACCTCCTCCTCACCGCCAATCCTCTCCCCATTTTCTCCCAAAACTCCGAATCTTTCCTCGCCGCCGCGAAGGAGTACGTCACGGCGGTGAAGAAGCTAAGCTGCGAAGTGGCGGAATTGATAGCAGAGGGATTGAAAATCGAGCCACGAAACGCAATAAGCAAGCTTCTAAACGACGAGAAAAGCGACTGTTGTTTCAGAGTGAACCACTACCCGCCATGTCCGGACCTCCAAGCTCTAGGCGGAAGTGGAAGGAACATGATCGGATTCGGGGAACACACAGACCCACAAATCATATCGCTGCTAAGATCAAACAACTCGACCGGCCTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCGGACGCGGCGGCGATCTTCGTGAACGTCGGCGACTCGCTGCAGGTGATGAGCAACGGCAGATTCAAAAGCGTGAAGCACAAAGTGGTGGCGGATTCCAACAGAGAGAGGGTTTCGATGATTTACTTCGGAGGGCCGCCGTTGAGTGAAAAGATTGCGCCATTGCCGGAGGTTTTGGGCGACGGAGAAGAAAGCTTGTACAAGGAATTCACTTGGTGGGAATACAAAACGGCGGCGTATAAGACCAGATTGGCTGATTACCGCCTCGGTCCCTTTGAGAAGAATCAAAATTCTTAG

Coding sequence (CDS)

ATGGTTGTTCTGTCACAGCCAGCATTGGGTTTAGACCAATATTCTCTTCTTCGAACAACATGCAAACCCACCGCCTCCTTCACCGAAATCCCTGAAATCGACCTCTCAGACCCCAACGCCCAGTCCCACATCGTCGAGGCCTGTGAAGAATTCGGCTTCTTTAAGCTCGTAAACCATCGAGTTCCGATTGAATTGATGACCAAACTCGAAGAAGAAGCCCTCTGTTTTTTCAAACTTTCCCAGTCTGAGAAAGATAAAGCTCGCCCTCCTGATCCTTTAGGCTATGGCTGCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATTGAATACCTCCTCCTCACCGCCAATCCTCTCCCCATTTTCTCCCAAAACTCCGAATCTTTCCTCGCCGCCGCGAAGGAGTACGTCACGGCGGTGAAGAAGCTAAGCTGCGAAGTGGCGGAATTGATAGCAGAGGGATTGAAAATCGAGCCACGAAACGCAATAAGCAAGCTTCTAAACGACGAGAAAAGCGACTGTTGTTTCAGAGTGAACCACTACCCGCCATGTCCGGACCTCCAAGCTCTAGGCGGAAGTGGAAGGAACATGATCGGATTCGGGGAACACACAGACCCACAAATCATATCGCTGCTAAGATCAAACAACTCGACCGGCCTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCGGACGCGGCGGCGATCTTCGTGAACGTCGGCGACTCGCTGCAGGTGATGAGCAACGGCAGATTCAAAAGCGTGAAGCACAAAGTGGTGGCGGATTCCAACAGAGAGAGGGTTTCGATGATTTACTTCGGAGGGCCGCCGTTGAGTGAAAAGATTGCGCCATTGCCGGAGGTTTTGGGCGACGGAGAAGAAAGCTTGTACAAGGAATTCACTTGGTGGGAATACAAAACGGCGGCGTATAAGACCAGATTGGCTGATTACCGCCTCGGTCCCTTTGAGAAGAATCAAAATTCTTAG

Protein sequence

MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHRVPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLYKEFTWWEYKTAAYKTRLADYRLGPFEKNQNS

Homology

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BLAST of Spg012011 vs. NCBI nr
Match: AUB30295.1 (gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca])

HSP 1 Score: 682.9 bits (1761), Expect = 1.3e-192
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of Spg012011 vs. NCBI nr
Match: XP_038883200.1 (gibberellin 2-beta-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 284/331 (85.80%), Postives = 310/331 (93.66%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L+QYSLLR+TCKPTASF +IPEIDLSDPNA+SHI++ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VP+ELMTKLE+E+LCFFKLS+SEKDKARPP PLGYGCKNIGSNGDKGWIEYLLL ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           I SQN  SFL AA+EYV AVKKLS EV ELIAEGLK+EPRN ISKLLNDEK+DCCFRVNH
Sbjct: 121 ILSQN--SFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCP++Q+LG  GRNMIGFGEHTDPQIIS+LRSNNS+GLQICLRDGAWVSVPPDA A+F
Sbjct: 181 YPPCPEMQSLG--GRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVVAD NRERVSMIYFGGPPLSEKI PLP++L +GEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFTWWEYKTAAYKT+LADYRLG FEK+ NS
Sbjct: 301 KEFTWWEYKTAAYKTKLADYRLGAFEKSPNS 327

[Close]

BLAST of Spg012011 vs. NCBI nr
Match: XP_008439633.1 (PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo])

HSP 1 Score: 584.7 bits (1506), Expect = 4.9e-163
Identity = 281/328 (85.67%), Postives = 303/328 (92.38%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L+QYSLLR+TCKPTASF +IPEIDLSDPNA+ HIV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VPIELMTKLE+E+LCFFKLS+SEKDKARPP PLGYG KNIGSNGDKGWIEYLL+ ANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IFS +   FL AA EYVTAVKKLSCEV ELIAEGLK+E RNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSHD---FLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCP++Q L  SGRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD  A+F
Sbjct: 181 YPPCPEMQGL--SGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVV D NRERVSMIYFGGPPLSEKI PLPE+L DGEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKN 329
           KEFTWWEYKTAAYK++LADYRLG FEK+
Sbjct: 301 KEFTWWEYKTAAYKSKLADYRLGAFEKS 323

[Close]

BLAST of Spg012011 vs. NCBI nr
Match: KAA0052541.1 (gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa] >TYK13284.1 gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 584.3 bits (1505), Expect = 6.4e-163
Identity = 281/328 (85.67%), Postives = 303/328 (92.38%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L+QYSLLR+TCKPTASF +IPEIDLSDPNA+ HIV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VPIELMTKLE+E+LCFFKLS+SEKDKARPP PLGYG KNIGSNGDKGWIEYLL+ ANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IFS +   FL AA EYVTAVKKLSCEV ELIAEGLK+E RNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSHD---FLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCP++Q L  SGRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD  A+F
Sbjct: 181 YPPCPEMQGL--SGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVV D NRERVSMIYFGGPPLSEKI PLPE+L DGEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKN 329
           KEFTWWEYKTAAYK++LADYRLG FEK+
Sbjct: 301 KEFTWWEYKTAAYKSKLADYRLGAFEKS 323

[Close]

BLAST of Spg012011 vs. NCBI nr
Match: XP_022977872.1 (gibberellin 2-beta-dioxygenase [Cucurbita maxima])

HSP 1 Score: 581.6 bits (1498), Expect = 4.2e-162
Identity = 278/331 (83.99%), Postives = 306/331 (92.45%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPAL L++YSLLR+T KP AS  +IPEIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VP+ELMTKLE+E+LCFFKLSQSEKDKARPPDPLGYG KNIGSNGDKGWIEYLLL ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           +FSQ S+SFL+AA EYVTAVKKLS EV ELI EGLKIEP+NAIS+L+NDEK+DC FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCP++QA+  SGRNMIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD  A F
Sbjct: 181 YPPCPEMQAV--SGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVVADS++ERVSMIYFGGPPLSEKI PL +VL +GEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFTWWEYKTAA+KTRLADYRLG FEK+Q S
Sbjct: 301 KEFTWWEYKTAAFKTRLADYRLGAFEKSQKS 329

[Close]

BLAST of Spg012011 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 2.2e-121
Identity = 216/335 (64.48%), Postives = 257/335 (76.72%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPA  L+Q+ LL+   K T  FT IP +DL+ P+A++ IV AC +FGFFKLVNH 
Sbjct: 1   MVVLSQPA--LNQFFLLK-PFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHG 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANP-- 120
           VP+ELM  LE EAL FFK SQSEKD+A PPDP GYG K IG NGD GW+EYLLL  NP  
Sbjct: 61  VPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDV 120

Query: 121 -----LPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSD 180
                L IF +N   F A  + Y+TAVK +   V EL+AEGL I  RN +S+LL DEKSD
Sbjct: 121 ISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSD 180

Query: 181 CCFRVNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVP 240
            CFR+NHYPPCP++QAL    RN++GFGEHTDPQIIS+LRSN+++GLQICL DG WVSVP
Sbjct: 181 SCFRLNHYPPCPEVQAL---NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVP 240

Query: 241 PDAAAIFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLG 300
           PD  + F+NVGD+LQVM+NGRFKSVKH+V+AD+ + R+SMIYFGGP LSE IAPLP V+ 
Sbjct: 241 PDQTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVML 300

Query: 301 DGEESLYKEFTWWEYKTAAYKTRLADYRLGPFEKN 329
            GEE LYKEFTW EYK AAY +RLAD RL PF+K+
Sbjct: 301 KGEECLYKEFTWCEYKKAAYTSRLADNRLAPFQKS 329

[Close]

BLAST of Spg012011 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 2.8e-108
Identity = 188/330 (56.97%), Postives = 255/330 (77.27%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MV+LS+P    +QY+ +R     T S + IP +DLS P+A++ IV+ACE+FGFFK++NH 
Sbjct: 1   MVLLSKPT--SEQYTYVRNNMPITFS-SSIPLVDLSKPDAKTLIVKACEDFGFFKVINHG 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANP-- 120
           +P++ +++LE EA  FF L Q+EK+KA P +P GYG K IG NGD GWIEYLLLT N   
Sbjct: 61  IPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQDH 120

Query: 121 -LPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFR 180
              ++ ++ + F    K+Y  A++ ++CE+ +L+AEGLKI+P+N  SKL+ D++SDC FR
Sbjct: 121 NFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFR 180

Query: 181 VNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAA 240
           VNHYP CP+L     +G N+IGFGEHTDPQIIS+LRSNN++G QI LRDG+W+SVPPD +
Sbjct: 181 VNHYPACPELAI---NGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHS 240

Query: 241 AIFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEE 300
           + F+NVGDSLQVM+NGRFKSV+H+V+A+    R+SMIYF GPPLSEKIAPLP ++  G+E
Sbjct: 241 SFFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKE 300

Query: 301 SLYKEFTWWEYKTAAYKTRLADYRLGPFEK 328
           SLYKEFTW+EYK++ Y +RLAD RLG +E+
Sbjct: 301 SLYKEFTWFEYKSSTYGSRLADNRLGNYER 323

[Close]

BLAST of Spg012011 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.1e-107
Identity = 193/336 (57.44%), Postives = 251/336 (74.70%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFT--EIPEIDLSDPNAQSHIVEACEEFGFFKLVN 60
           MVVL QP + LD +  L  T KP    T   IP ++L+DP A++ IV+ACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANP 120
           H V  ELMT+LE+EA+ FF L QS K++A PP+P GYG K IG NGD GWIEYLLL ANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEK 180
                    +F Q  + F  + +EY+  +K++S +V E++AE L IEPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 SDCCFRVNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVS 240
           SD C R+NHYP   +           +GFGEHTDPQIIS+LRSNN+ GLQIC++DG+WV+
Sbjct: 181 SDSCLRLNHYPAAEE----EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVA 240

Query: 241 VPPDAAAIFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEV 300
           VPPD ++ F+NVGD+LQVM+NGRFKSVKH+V+AD+ R R+SMIYFGGPPLS+KIAPLP +
Sbjct: 241 VPPDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCL 300

Query: 301 LGDGEESLYKEFTWWEYKTAAYKTRLADYRLGPFEK 328
           + + ++ LYKEFTW +YK++AYK++L DYRLG FEK
Sbjct: 301 VPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Spg012011 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.9e-99
Identity = 183/331 (55.29%), Postives = 233/331 (70.39%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVL+ P   +D   LLR+   P   F+ +P +DL  P A   +V+ACE +GFFK+VNH 
Sbjct: 1   MVVLAGPP-AVDHIPLLRSP-DPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVVNHG 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           V  + M K E EA+ FF  +Q +KD++ P  P GYG K IG NGD GW+EYLLL  +   
Sbjct: 61  VATDTMDKAESEAVRFFSQTQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDAS 120

Query: 121 IFS----QNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCF 180
           +       +   F AA  EY++ V+K++  V E ++EGL I   +A+S L+  E SD  F
Sbjct: 121 LADACTVPSCAVFRAALNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVF 180

Query: 181 RVNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDA 240
           RVNHYPPC  LQ LG S   + GFGEHTDPQ++S+LRSN ++GLQI LRDG WVSVP D 
Sbjct: 181 RVNHYPPCRALQGLGCS---VTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDR 240

Query: 241 AAIFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGE 300
            + FVNVGDSLQV++NGRFKSVKH+VVA+S + RVS IYFGGPPL+++IAPLP++LG+GE
Sbjct: 241 DSFFVNVGDSLQVLTNGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGE 300

Query: 301 ESLYKEFTWWEYKTAAYKTRLADYRLGPFEK 328
           +SLYKEFTW EYK AAYK+RL D RL  FEK
Sbjct: 301 QSLYKEFTWDEYKKAAYKSRLGDNRLAQFEK 326

[Close]

BLAST of Spg012011 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 7.5e-98
Identity = 178/334 (53.29%), Postives = 232/334 (69.46%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MV++ QPA   D    +   CKP      IP IDL+D +A++ IV+ACEEFGFFK++NH 
Sbjct: 1   MVIVLQPA-SFDSNLYVNPKCKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINHG 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTAN--- 120
           V  +L+T+LE+EA+ FF L  S KDKA PPDP GYG K IG NGD GW+EY+LL AN   
Sbjct: 61  VRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCL 120

Query: 121 ----PLPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSD 180
                  IF      F  A +EY+  +K++S +  E++ E LKIEP+  +S+L+  ++SD
Sbjct: 121 ESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESD 180

Query: 181 CCFRVNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVP 240
            C R+NHYP   +        +  IGFGEHTDPQ+ISLLRSN++ GLQIC++DG WV V 
Sbjct: 181 SCLRMNHYPEKEETPV-----KEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVT 240

Query: 241 PDAAAIFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLG 300
           PD ++ FV VGD+LQVM+NGRFKSVKH+VV ++ R R+SMIYF GPPLSEKIAPL  ++ 
Sbjct: 241 PDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVP 300

Query: 301 DGEESLYKEFTWWEYKTAAYKTRLADYRLGPFEK 328
             ++ LY EFTW +YK +AYKT+L DYRLG FEK
Sbjct: 301 KQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Spg012011 vs. ExPASy TrEMBL
Match: A0A2H4V3T7 (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 6.5e-193
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of Spg012011 vs. ExPASy TrEMBL
Match: A0A1S3AZ81 (gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.4e-163
Identity = 281/328 (85.67%), Postives = 303/328 (92.38%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L+QYSLLR+TCKPTASF +IPEIDLSDPNA+ HIV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VPIELMTKLE+E+LCFFKLS+SEKDKARPP PLGYG KNIGSNGDKGWIEYLL+ ANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IFS +   FL AA EYVTAVKKLSCEV ELIAEGLK+E RNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSHD---FLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCP++Q L  SGRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD  A+F
Sbjct: 181 YPPCPEMQGL--SGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVV D NRERVSMIYFGGPPLSEKI PLPE+L DGEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKN 329
           KEFTWWEYKTAAYK++LADYRLG FEK+
Sbjct: 301 KEFTWWEYKTAAYKSKLADYRLGAFEKS 323

[Close]

BLAST of Spg012011 vs. ExPASy TrEMBL
Match: A0A5A7UGB1 (Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009030 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.1e-163
Identity = 281/328 (85.67%), Postives = 303/328 (92.38%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQP L L+QYSLLR+TCKPTASF +IPEIDLSDPNA+ HIV+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VPIELMTKLE+E+LCFFKLS+SEKDKARPP PLGYG KNIGSNGDKGWIEYLL+ ANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           IFS +   FL AA EYVTAVKKLSCEV ELIAEGLK+E RNAISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSHD---FLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCP++Q L  SGRNMIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD  A+F
Sbjct: 181 YPPCPEMQGL--SGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVV D NRERVSMIYFGGPPLSEKI PLPE+L DGEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKN 329
           KEFTWWEYKTAAYK++LADYRLG FEK+
Sbjct: 301 KEFTWWEYKTAAYKSKLADYRLGAFEKS 323

[Close]

BLAST of Spg012011 vs. ExPASy TrEMBL
Match: A0A6J1IJN1 (gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 278/331 (83.99%), Postives = 306/331 (92.45%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPAL L++YSLLR+T KP AS  +IPEIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VP+ELMTKLE+E+LCFFKLSQSEKDKARPPDPLGYG KNIGSNGDKGWIEYLLL ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           +FSQ S+SFL+AA EYVTAVKKLS EV ELI EGLKIEP+NAIS+L+NDEK+DC FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCP++QA+  SGRNMIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD  A F
Sbjct: 181 YPPCPEMQAV--SGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVVADS++ERVSMIYFGGPPLSEKI PL +VL +GEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFTWWEYKTAA+KTRLADYRLG FEK+Q S
Sbjct: 301 KEFTWWEYKTAAFKTRLADYRLGAFEKSQKS 329

[Close]

BLAST of Spg012011 vs. ExPASy TrEMBL
Match: I0J1J0 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 278/331 (83.99%), Postives = 306/331 (92.45%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MVVLSQPAL L++YSLLR+T KP AS  +IPEIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120
           VP+ELMTKLE+E+LCFFKLSQSEKDKARPPDPLGYG KNIGSNGDKGWIEYLLL ANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180
           +FSQ S+SFL+AA EYVTAVKKLS EV ELI EGLKIEP+NAIS+L+NDEK+DC FRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240
           YPPCP++QA+  SGRNMIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD  A F
Sbjct: 181 YPPCPEMQAV--SGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVVADS++ERVSMIYFGGPPLSEKI PL +VL +GEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLY 300

Query: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFTWWEYKTAA+KTRLADYRLG FEK+Q S
Sbjct: 301 KEFTWWEYKTAAFKTRLADYRLGAFEKSQKS 329

[Close]

BLAST of Spg012011 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 391.3 bits (1004), Expect = 7.5e-109
Identity = 193/336 (57.44%), Postives = 251/336 (74.70%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFT--EIPEIDLSDPNAQSHIVEACEEFGFFKLVN 60
           MVVL QP + LD +  L  T KP    T   IP ++L+DP A++ IV+ACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANP 120
           H V  ELMT+LE+EA+ FF L QS K++A PP+P GYG K IG NGD GWIEYLLL ANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEK 180
                    +F Q  + F  + +EY+  +K++S +V E++AE L IEPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 SDCCFRVNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVS 240
           SD C R+NHYP   +           +GFGEHTDPQIIS+LRSNN+ GLQIC++DG+WV+
Sbjct: 181 SDSCLRLNHYPAAEE----EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVA 240

Query: 241 VPPDAAAIFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEV 300
           VPPD ++ F+NVGD+LQVM+NGRFKSVKH+V+AD+ R R+SMIYFGGPPLS+KIAPLP +
Sbjct: 241 VPPDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCL 300

Query: 301 LGDGEESLYKEFTWWEYKTAAYKTRLADYRLGPFEK 328
           + + ++ LYKEFTW +YK++AYK++L DYRLG FEK
Sbjct: 301 VPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Spg012011 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 358.6 bits (919), Expect = 5.4e-99
Identity = 178/334 (53.29%), Postives = 232/334 (69.46%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60
           MV++ QPA   D    +   CKP      IP IDL+D +A++ IV+ACEEFGFFK++NH 
Sbjct: 1   MVIVLQPA-SFDSNLYVNPKCKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINHG 60

Query: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTAN--- 120
           V  +L+T+LE+EA+ FF L  S KDKA PPDP GYG K IG NGD GW+EY+LL AN   
Sbjct: 61  VRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCL 120

Query: 121 ----PLPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSD 180
                  IF      F  A +EY+  +K++S +  E++ E LKIEP+  +S+L+  ++SD
Sbjct: 121 ESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESD 180

Query: 181 CCFRVNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVP 240
            C R+NHYP   +        +  IGFGEHTDPQ+ISLLRSN++ GLQIC++DG WV V 
Sbjct: 181 SCLRMNHYPEKEETPV-----KEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVT 240

Query: 241 PDAAAIFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLG 300
           PD ++ FV VGD+LQVM+NGRFKSVKH+VV ++ R R+SMIYF GPPLSEKIAPL  ++ 
Sbjct: 241 PDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVP 300

Query: 301 DGEESLYKEFTWWEYKTAAYKTRLADYRLGPFEK 328
             ++ LY EFTW +YK +AYKT+L DYRLG FEK
Sbjct: 301 KQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Spg012011 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 345.5 bits (885), Expect = 4.7e-95
Identity = 166/312 (53.21%), Postives = 226/312 (72.44%), Query Frame = 0


Query: 23  PTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHRVPIELMTKLEEEALCFFKLSQS 82
           P + F+ IP ID+SDP ++  +V+ACE+FGFFK++NH V  EL++ LE E + FF L +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTAN------PL-PIFSQNSESFLAAAKE 142
           EK +     P GYG   IG NGD GW+EYLL+ AN      PL P   ++  +F  A +E
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNHYPPCPDLQALGGSGR 202
           Y T+V+K++ +V E I +GL I+PRN +SKL++D+ +D   R+NHYPPCP        G+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIFVNVGDSLQVMSNGRF 262
           N+IGFGEHTDPQIIS+LRSNN++GLQI L DG+W+SVPPD  + F NVGDSLQVM+NGRF
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 250

Query: 263 KSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLYKEFTWWEYKTAAYKT 322
           KSV+H+V+A+  + RVSMIYF GP L+++IAPL  ++ + +E LY+EFTW EYK + Y +
Sbjct: 251 KSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNS 310

Query: 323 RLADYRLGPFEK 328
           RL+D RL  FE+
Sbjct: 311 RLSDNRLQQFER 321

[Close]

BLAST of Spg012011 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 275.8 bits (704), Expect = 4.6e-74
Identity = 140/258 (54.26%), Postives = 182/258 (70.54%), Query Frame = 0


Query: 1   MVVLSQPALGLDQYSLLRTTCKPTASFT--EIPEIDLSDPNAQSHIVEACEEFGFFKLVN 60
           MVVL QP + LD +  L  T KP    T   IP ++L+DP A++ IV+ACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANP 120
           H V  ELMT+LE+EA+ FF L QS K++A PP+P GYG K IG NGD GWIEYLLL ANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEK 180
                    +F Q  + F  + +EY+  +K++S +V E++AE L IEPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 SDCCFRVNHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVS 240
           SD C R+NHYP   +           +GFGEHTDPQIIS+LRSNN+ GLQIC++DG+WV+
Sbjct: 181 SDSCLRLNHYPAAEE----EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVA 240

Query: 241 VPPDAAAIFVNVGDSLQV 250
           VPPD ++ F+NVGD+LQV
Sbjct: 241 VPPDHSSFFINVGDALQV 253

[Close]

BLAST of Spg012011 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 273.1 bits (697), Expect = 3.0e-73
Identity = 146/323 (45.20%), Postives = 201/323 (62.23%), Query Frame = 0


Query: 17  LRTTCKPTASFTE--IPEID--LSDPNAQSH-IVEACEEFGFFKLVNHRVPIELMTKLEE 76
           L+TT K T S  E   P ID  L+D +  S  IV+ACE  GFFK++NH V  E++ + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 77  EALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLPIFSQNSE---- 136
           E   FF   +S+K +A P  P GYGCKNIG NGD G +EYLLL ANP  +  ++      
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHD 128

Query: 137 ---SFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNHYPPC 196
               F +A  +Y+  V+ L+CE+ +L  E L  +  + +S+L+ D +SD   R+NHYPP 
Sbjct: 129 DPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA 188

Query: 197 PDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIFVNVG 256
           P   AL G G+  IGFGEH+DPQI+++LRSN+  GL+IC RDG W+ +P D    FV VG
Sbjct: 189 P--YALSGVGQ--IGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVG 248

Query: 257 DSLQVMSNGRFKSVKHKVVAD-SNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLYKEF 316
           D LQ ++NGRF SV+H+V+A+ + + R+S +YF  PPL  KI+PLP+++       Y  F
Sbjct: 249 DCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSF 308

Query: 317 TWWEYKTAAYKTRLADYRLGPFE 327
           TW +YK A Y  RL   RL  F+
Sbjct: 309 TWGDYKKATYSLRLDVPRLEFFK 327

The following BLAST results are available for this feature:

BLAST of Spg012011 vs. NCBI nr


Match Name	E-value	Identity	Description
AUB30295.1	1.3e-192	100.00	gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca]	[more]
XP_038883200.1	1.6e-166	85.80	gibberellin 2-beta-dioxygenase-like [Benincasa hispida]	[more]
XP_008439633.1	4.9e-163	85.67	PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo]	[more]
KAA0052541.1	6.4e-163	85.67	gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa] >TYK13284.1 gibbe...	[more]
XP_022977872.1	4.2e-162	83.99	gibberellin 2-beta-dioxygenase [Cucurbita maxima]	[more]

BLAST of Spg012011 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	2.2e-121	64.48	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9SQ80	2.8e-108	56.97	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
Q9XFR9	1.1e-107	57.44	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q8S0S6	8.9e-99	55.29	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
O64692	7.5e-98	53.29	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]

BLAST of Spg012011 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2H4V3T7	6.5e-193	100.00	Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S...	[more]
A0A1S3AZ81	2.4e-163	85.67	gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3...	[more]
A0A5A7UGB1	3.1e-163	85.67	Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A6J1IJN1	2.0e-162	83.99	gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 ...	[more]
I0J1J0	2.0e-162	83.99	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1	[more]

BLAST of Spg012011 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	7.5e-109	57.44	gibberellin 2-oxidase	[more]
AT2G34555.1	5.4e-99	53.29	gibberellin 2-oxidase 3	[more]
AT1G78440.1	4.7e-95	53.21	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G30040.2	4.6e-74	54.26	gibberellin 2-oxidase	[more]
AT1G02400.1	3.0e-73	45.20	gibberellin 2-oxidase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 133..153
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 10..328
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 10..328
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 21..319
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 12..319 e-value: 3.4E-78 score: 265.2
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 30..111 e-value: 1.1E-12 score: 48.8
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 173..277 e-value: 1.7E-25 score: 89.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 171..279 score: 11.088902

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg012011.1	Spg012011.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding