Spg011973 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg011973
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionSeipin-2
Locationscaffold1: 2207421 .. 2210617 (+)
RNA-Seq ExpressionSpg011973
SyntenySpg011973
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCCCACGACACCAAAGATAACGAAGACGACGATGACTTCTTCGACACTCTCGACCATTTCCCCTCCGAGGATTCTTCCGTCGCCGATCAACCTCAACTTTCCACTTCACCCCCCTCCCCCTCCGATGAAATCTCGTCTGCGCATGCCCCCTCCCCTGTCAATTCATTGCGCCGTCGGCCCTCTGCTTCCCGCGGAATCGCAGGCGAGAATCCGACTTCAGATTCCTCCATCAGCTCCCCAACAAGTACAATCGACGATTCGATGAACATGAGCCCCGAAGGGAAAAACCCAGAAATTCACCGGGATTTTGATGACGGTGGAAAGAAACTCGAGGGATCCGAGTCTTTATCCGTTCAGGTTAGCTCGTCGGTGGGTTCGAGTAGCGTAAATGAAGAGAAGAGTGAGGTATCGACGGTGACCACCGCGGAGATTAACTCGGACGGCGAATTGGGCGTTTCGGAGGCGGAATCCGGCGATTCTTCTACAAGCATACTTGTGTTGATTGCAGGATTGCTCATCAAGGCAATTGGGGTTCAACTAAGCTTCTTCGTTTACAGTATCTGCTTTCCCCTGTGGTTTTTATATCTTTCTTACACGTTTGTTTTCCATCCTTTTCAAACAATTAAGCTTGGGAGAGCATATGTGACAGGAAAGTTATTTGGGGTTTGGGAGCTTGTTTTTGCTGTGGTCGGTCCTTTGATATCTGAACGGTTTAAGGAGCCCAAGTCTCTTTGGAAGGTTGGACTCCGCTGTGTGTGGGGTTTGTTGTGGTCGGCTTATGTTTGTATCATTTTGTGTGGTCTCTTGATTTCGGCGCTTATATTTAGTGGGTTTTTGATGAGGTTCTTAGTTCAGGAGCCTCTCAAGATGAAAGAAGTATTAAATTTTGACTACACCAAACACAGTCCTGAGGCTTACATGCCAATCTTGCCTGATTCAAATTATCTTTATGGTCGGAATTGTCAAGAGAATGCCGTAAGTGGGAAGACCCAGTCGCGAGCTATTCCTCCTCATCATAATTTGCAGGTCACTGTCTCACTAACATTGCCAGAGTCTGAATATAACAGAAATCTTGGGGTCTTCCAGGTATGTCTTTATTTCTTTTGTCAAGGGAATATGGTTTTTAATTATGACAAGTAGTATAATTTTCTGATTCTTTTATTGTTATGTGAAGCATTGATCTTCGTTGAAGTTGGATGATATTATTTTCTCAGATTCAAGAAGTGAACTTGATATAATCATTACATAACTCGAATGGATAAGATTGGGAATGAGGACCAAATTCCTTTTTTGCCTGCCTGTAACCATTGTACGTGCTGACGTTGGACTTTGCAGTTATTCAGTTGAAGAAACTACTAAGAAAGTGTGCTTCTGAGATGAGAATCAATCATTTTGTCTAAGCTTCAAGGATCAATTTGCTGGATATAGGATGATATGGGAACAGTAGTAATATTCAATGACATAGGAACTGCTAAGAACTTGATTTTCCATGAATTATGAATAAAGTAAACAAGAAAAGGCCACATTAAAATAAGTTCAAAACAGAGGATCCCCCTCCAAAAAAAATAAAGCTAATGTAGAGTGGTTTACAACCTTACTTGAACAGGATCTGTTCTATAGGTTGTACGTGTCCTTGAGTTCTAAAGCTAATATAGTAGTGGTTTCAGTTGTTTGAGTATAAAAAACAACCGAATATTAAAGAAAAATTTGTAGGGAACATCCAAAGAGTTTGCCACCTATGTGCGAATGATGAAGGTGAGAGGGCTTATAGAATCTTGCTTAGGATCAGACAACTGCACCTGAACTTCTATATATTATTAAATTTAGTCAGTGTTTGATTTTAATTTGTCTGAAAGTTCTATTTTTAGTTAACAGTCTCATCACTGGATGTATATACTTTCTCTCAAGTCTGTTTGGGTAATGTGTATTATGTGAATCCCTGTGAGATATTTGATGGCTATGCTGCAGGTAAGAGTAGACTTTCTTTCTGTTAGTGGTGACATTCTTGCAAGCTCAAGCCATCCATGCATGTTACAGTTTAAAAGTGAGCCCATTCGCCTTCTATTGACTATCCTGAAGCTTGCACCCCTTGTTACTGGCTATATATCAGAGTCCCAGACTCTGAATCTTGAGCTTAAAGGTTTCACAGAAGGAGACATACCGACAGCCTGTCTGCGAGTTACAATTGAACAGCGAGCAGAGTTCAATCCTGGTGCTGGTATACCCGAAATATATGATGCATCCTTAATCCTTGAATCCGAACTTCCCCTGTTCAAAAGGATTATATGGTACTGGAGGAAGACTATCTATGTATGGATTAGCATGACATCGTTTATGATGCAGTTGCTTTTTATGCTGGTCTGTTGTCGACCAATCATCCTTCCTCGAATAAGGAGAAGGGATGGATCTGCCAATGCAAGGTCCATAAGCAACATCTATAGACGAGGTTAGATAAGAACAGGGCCACCTGGATATCTGATCGAAATGTTCAATCAATGAATTTTGTTGCTGATGTATAACTATAAACCCAACAATTCCAACGTTGTTGGTTGCTCTAATACCGAATACTACATATTTGGTACGACCAATTCTTTAATTAATTAATTTATTTTGTTGACGTGTATGTTCTTGGTCTGAAGTGAATGGTGATGATGAGAGAAGAGCAGACCATATTTATGTCTGTACAGTTTTAGAAACCATTTCAACTCAGCACGAACAAACCTGCAATAGACTGAAGCTCTGTTAACTGTTAACCGGGTTTTATCAGGGCTACACCATGGGCGAGGAGAAAAGAAGTCTCACTTGGAAGATACAAAACTTAAACTCTTTACATTGGAACCTTTTATTTGTCATCATCAGATTATTGTTTGATTTTTTTTTCTTTGAAATAAACTTGAAGCAAGTAACCTGTGCATTGATGAGCAGAGTTCATGTGAACTTCTCCGCAACCATCAAAATTTGAAACTTATTCTGATCTAGATGTTAGTTAACATAGAAGTAGAACAATTTGGTAACTGGACGCCCATCTACAGTAAACTTTGAAGCACAAGAAAGAAAAAAGAAAGGTCCTTTGAATTGTTTATATACTATTGCTTGCAGGTCCCAAGCTTCTGGCTGGATTCATGGATGCTCCTGTAATTGGCCTGAAAAACAGGCTATCAGATTACCCAGATAGAATATTGAGATTTTAA

mRNA sequence

ATGGAATCCCACGACACCAAAGATAACGAAGACGACGATGACTTCTTCGACACTCTCGACCATTTCCCCTCCGAGGATTCTTCCGTCGCCGATCAACCTCAACTTTCCACTTCACCCCCCTCCCCCTCCGATGAAATCTCGTCTGCGCATGCCCCCTCCCCTGTCAATTCATTGCGCCGTCGGCCCTCTGCTTCCCGCGGAATCGCAGGCGAGAATCCGACTTCAGATTCCTCCATCAGCTCCCCAACAAGTACAATCGACGATTCGATGAACATGAGCCCCGAAGGGAAAAACCCAGAAATTCACCGGGATTTTGATGACGGTGGAAAGAAACTCGAGGGATCCGAGTCTTTATCCGTTCAGGTTAGCTCGTCGGTGGGTTCGAGTAGCGTAAATGAAGAGAAGAGTGAGGTATCGACGGTGACCACCGCGGAGATTAACTCGGACGGCGAATTGGGCGTTTCGGAGGCGGAATCCGGCGATTCTTCTACAAGCATACTTGTGTTGATTGCAGGATTGCTCATCAAGGCAATTGGGGTTCAACTAAGCTTCTTCGTTTACAGTATCTGCTTTCCCCTGTGGTTTTTATATCTTTCTTACACGTTTGTTTTCCATCCTTTTCAAACAATTAAGCTTGGGAGAGCATATGTGACAGGAAAGTTATTTGGGGTTTGGGAGCTTGTTTTTGCTGTGGTCGGTCCTTTGATATCTGAACGGTTTAAGGAGCCCAAGTCTCTTTGGAAGGTTGGACTCCGCTGTGTGTGGGGTTTGTTGTGGTCGGCTTATGTTTGTATCATTTTGTGTGGTCTCTTGATTTCGGCGCTTATATTTAGTGGGTTTTTGATGAGGTTCTTAGTTCAGGAGCCTCTCAAGATGAAAGAAGTATTAAATTTTGACTACACCAAACACAGTCCTGAGGCTTACATGCCAATCTTGCCTGATTCAAATTATCTTTATGGTCGGAATTGTCAAGAGAATGCCGTAAGTGGGAAGACCCAGTCGCGAGCTATTCCTCCTCATCATAATTTGCAGGTCACTGTCTCACTAACATTGCCAGAGTCTGAATATAACAGAAATCTTGGGGTCTTCCAGGATGATATGGGAACAGTAGTAAGAGTAGACTTTCTTTCTGTTAGTGGTGACATTCTTGCAAGCTCAAGCCATCCATGCATGTTACAGTTTAAAAGTGAGCCCATTCGCCTTCTATTGACTATCCTGAAGCTTGCACCCCTTGTTACTGGCTATATATCAGAGTCCCAGACTCTGAATCTTGAGCTTAAAGGTTTCACAGAAGGAGACATACCGACAGCCTGTCTGCGAGTTACAATTGAACAGCGAGCAGAGTTCAATCCTGGTGCTGGTATACCCGAAATATATGATGCATCCTTAATCCTTGAATCCGAACTTCCCCTGTTCAAAAGGATTATATGGTACTGGAGGAAGACTATCTATGTATGGATTAGCATGACATCGTTTATGATGCAGTTGCTTTTTATGCTGGTCTGTTGTCGACCAATCATCCTTCCTCGAATAAGGAGAAGGGATGGATCTGCCAATGCAAGGTCCATAAGCAACATCTATAGACGAGTGAATGGTGATGATGAGAGAAGAGCAGACCATATTTATGTCTGTCCCAAGCTTCTGGCTGGATTCATGGATGCTCCTGTAATTGGCCTGAAAAACAGGCTATCAGATTACCCAGATAGAATATTGAGATTTTAA

Coding sequence (CDS)

ATGGAATCCCACGACACCAAAGATAACGAAGACGACGATGACTTCTTCGACACTCTCGACCATTTCCCCTCCGAGGATTCTTCCGTCGCCGATCAACCTCAACTTTCCACTTCACCCCCCTCCCCCTCCGATGAAATCTCGTCTGCGCATGCCCCCTCCCCTGTCAATTCATTGCGCCGTCGGCCCTCTGCTTCCCGCGGAATCGCAGGCGAGAATCCGACTTCAGATTCCTCCATCAGCTCCCCAACAAGTACAATCGACGATTCGATGAACATGAGCCCCGAAGGGAAAAACCCAGAAATTCACCGGGATTTTGATGACGGTGGAAAGAAACTCGAGGGATCCGAGTCTTTATCCGTTCAGGTTAGCTCGTCGGTGGGTTCGAGTAGCGTAAATGAAGAGAAGAGTGAGGTATCGACGGTGACCACCGCGGAGATTAACTCGGACGGCGAATTGGGCGTTTCGGAGGCGGAATCCGGCGATTCTTCTACAAGCATACTTGTGTTGATTGCAGGATTGCTCATCAAGGCAATTGGGGTTCAACTAAGCTTCTTCGTTTACAGTATCTGCTTTCCCCTGTGGTTTTTATATCTTTCTTACACGTTTGTTTTCCATCCTTTTCAAACAATTAAGCTTGGGAGAGCATATGTGACAGGAAAGTTATTTGGGGTTTGGGAGCTTGTTTTTGCTGTGGTCGGTCCTTTGATATCTGAACGGTTTAAGGAGCCCAAGTCTCTTTGGAAGGTTGGACTCCGCTGTGTGTGGGGTTTGTTGTGGTCGGCTTATGTTTGTATCATTTTGTGTGGTCTCTTGATTTCGGCGCTTATATTTAGTGGGTTTTTGATGAGGTTCTTAGTTCAGGAGCCTCTCAAGATGAAAGAAGTATTAAATTTTGACTACACCAAACACAGTCCTGAGGCTTACATGCCAATCTTGCCTGATTCAAATTATCTTTATGGTCGGAATTGTCAAGAGAATGCCGTAAGTGGGAAGACCCAGTCGCGAGCTATTCCTCCTCATCATAATTTGCAGGTCACTGTCTCACTAACATTGCCAGAGTCTGAATATAACAGAAATCTTGGGGTCTTCCAGGATGATATGGGAACAGTAGTAAGAGTAGACTTTCTTTCTGTTAGTGGTGACATTCTTGCAAGCTCAAGCCATCCATGCATGTTACAGTTTAAAAGTGAGCCCATTCGCCTTCTATTGACTATCCTGAAGCTTGCACCCCTTGTTACTGGCTATATATCAGAGTCCCAGACTCTGAATCTTGAGCTTAAAGGTTTCACAGAAGGAGACATACCGACAGCCTGTCTGCGAGTTACAATTGAACAGCGAGCAGAGTTCAATCCTGGTGCTGGTATACCCGAAATATATGATGCATCCTTAATCCTTGAATCCGAACTTCCCCTGTTCAAAAGGATTATATGGTACTGGAGGAAGACTATCTATGTATGGATTAGCATGACATCGTTTATGATGCAGTTGCTTTTTATGCTGGTCTGTTGTCGACCAATCATCCTTCCTCGAATAAGGAGAAGGGATGGATCTGCCAATGCAAGGTCCATAAGCAACATCTATAGACGAGTGAATGGTGATGATGAGAGAAGAGCAGACCATATTTATGTCTGTCCCAAGCTTCTGGCTGGATTCATGGATGCTCCTGTAATTGGCCTGAAAAACAGGCTATCAGATTACCCAGATAGAATATTGAGATTTTAA

Protein sequence

MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLSTSPPSPSDEISSAHAPSPVNSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGSESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLIKAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPLISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEVLNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESEYNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRIIWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILPRIRRRDGSANARSISNIYRRVNGDDERRADHIYVCPKLLAGFMDAPVIGLKNRLSDYPDRILRF
Homology
BLAST of Spg011973 vs. NCBI nr
Match: XP_038883173.1 (seipin-2-like [Benincasa hispida])

HSP 1 Score: 824.3 bits (2128), Expect = 6.4e-235
Identity = 441/519 (84.97%), Postives = 469/519 (90.37%), Query Frame = 0

Query: 1   MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLSTSP---PSPSDEISSAHAPSPVNS 60
           MESHDTKDNEDDDDF DTL  FPSE+ SV DQPQLSTSP    SP  EISS +AP PVNS
Sbjct: 1   MESHDTKDNEDDDDFLDTLHDFPSENCSVIDQPQLSTSPSSDSSPLPEISSENAPFPVNS 60

Query: 61  LRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGSES 120
           LRRR S  R IA E P+SDSSISS T+TIDDS+  +PE KNPEIH DF+D GKKLEGSES
Sbjct: 61  LRRRSSVRRRIAAEIPSSDSSISSLTTTIDDSVKTNPERKNPEIHWDFNDDGKKLEGSES 120

Query: 121 LSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLIKA 180
           LSVQV+SS GSSSVNEE +EVSTVTTAEINSD +LG+SE ES DSS+SILVLIAGLLIKA
Sbjct: 121 LSVQVNSSAGSSSVNEENTEVSTVTTAEINSDVQLGISEVESSDSSSSILVLIAGLLIKA 180

Query: 181 IGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPLIS 240
           IGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYV GKLFGVW+LV AVVG L+S
Sbjct: 181 IGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVRGKLFGVWDLVTAVVGRLVS 240

Query: 241 ERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEVLN 300
           ERFKE KSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFS F MRFLVQEP+KMKEVLN
Sbjct: 241 ERFKERKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSAFFMRFLVQEPMKMKEVLN 300

Query: 301 FDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESEYN 360
           FDYTKHSPEA MPILPDSN LYG NC++N V+GKTQ R IPPHH LQV VSLTLPESEYN
Sbjct: 301 FDYTKHSPEALMPILPDSNDLYGHNCKDNVVTGKTQYRVIPPHHQLQVIVSLTLPESEYN 360

Query: 361 RNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYIS 420
           RNLGVFQ      VRVDFLSVSG+ILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYIS
Sbjct: 361 RNLGVFQ------VRVDFLSVSGNILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYIS 420

Query: 421 ESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRIIW 480
           ESQTLN++LKGFTEG+IPTACLRVTIEQRAEF+PGAGIPEIY+AS+ILESELPLFKRIIW
Sbjct: 421 ESQTLNIKLKGFTEGNIPTACLRVTIEQRAEFDPGAGIPEIYNASVILESELPLFKRIIW 480

Query: 481 YWRKTIYVWISMTSFMMQLLFMLVCCRPIILPRIRRRDG 517
           YWRKTIYVWISMTSFMMQLLF+LVCCRPIILPR+RRR+G
Sbjct: 481 YWRKTIYVWISMTSFMMQLLFILVCCRPIILPRMRRRNG 513

BLAST of Spg011973 vs. NCBI nr
Match: XP_023003136.1 (seipin-2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 807.0 bits (2083), Expect = 1.1e-229
Identity = 440/532 (82.71%), Postives = 465/532 (87.41%), Query Frame = 0

Query: 1   MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLSTSPPSPSD-----EISSAHAPSPV 60
           MESHDTKDNEDDDDFFDTLD FPSEDSSV DQPQLSTS  S SD     E SS +  S V
Sbjct: 1   MESHDTKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSPVLETSSENEFSSV 60

Query: 61  NSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGS 120
            SLR RPS+ R +AGE P+SDSSISS TS+IDDS+ MSPE K PEIH +F D  KKLEGS
Sbjct: 61  ASLRHRPSSRRRMAGETPSSDSSISSLTSSIDDSVMMSPEWKTPEIHPNFIDDVKKLEGS 120

Query: 121 ESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLI 180
           E+LSVQVSS  GSSSV +EKSEVSTVTT E NS GELGVSEAESG SS+++L+LI GLLI
Sbjct: 121 EALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGLLI 180

Query: 181 KAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPL 240
           KAIGVQLSFFVYS CFPLWFLY SYTFVFHPFQTIKLGRAYVTGKLFG  ELV AVV PL
Sbjct: 181 KAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVSPL 240

Query: 241 ISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEV 300
           ISER KEPKSLWK GLRCVWG LWSAYVCIILCGLLISALIF GFLMRFLVQEP+K+KEV
Sbjct: 241 ISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIKEV 300

Query: 301 LNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESE 360
           LNFDYTKHSPEAYMPILPDS+ LYGRNC+EN VSGKTQSR IP HH+LQ  VSLTLPESE
Sbjct: 301 LNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKTQSRVIPHHHHLQAIVSLTLPESE 360

Query: 361 YNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420
           YNRNLGVFQ      VRVDFLSVSGD+LASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY
Sbjct: 361 YNRNLGVFQ------VRVDFLSVSGDVLASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420

Query: 421 ISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRI 480
           ISESQTLNL+LKG  EG++PTACLRVTIEQRAEF+PGAGIPEIYDASLILESELPLFKRI
Sbjct: 421 ISESQTLNLKLKGLAEGNVPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRI 480

Query: 481 IWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILP--RIRRRDGSANARSISN 526
           IWYWRKT+YVWISMTSFMMQLLFMLVCCRPIILP  R+RRRD SAN  S  N
Sbjct: 481 IWYWRKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDESANVSSTRN 526

BLAST of Spg011973 vs. NCBI nr
Match: KAA0052594.1 (seipin-2 [Cucumis melo var. makuwa] >TYK13231.1 seipin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 804.7 bits (2077), Expect = 5.3e-229
Identity = 433/526 (82.32%), Postives = 465/526 (88.40%), Query Frame = 0

Query: 1   MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLST-----SPPSPSDEISSAHAPSPV 60
           MESHDTKDNEDDDD FD+   FPSE+ S+ADQPQLST     S  SP  EISS + P+PV
Sbjct: 4   MESHDTKDNEDDDDLFDSFHDFPSENCSLADQPQLSTSTSPSSDSSPLPEISSENVPAPV 63

Query: 61  NSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGS 120
           NS RRRPS  R IAGE PTSDSSISS T+TIDDS+  +PE K+PEIH DF+D G KLEG 
Sbjct: 64  NSFRRRPSVRRRIAGETPTSDSSISSLTTTIDDSVK-NPERKSPEIHWDFNDDGNKLEGP 123

Query: 121 ESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLI 180
           ESLSVQV+S  GSSSVNE+ +E STVTTAEINS+ ELG SE ES DSS+SILVLIAGLLI
Sbjct: 124 ESLSVQVNSWAGSSSVNEKDTEASTVTTAEINSEVELGASEVESRDSSSSILVLIAGLLI 183

Query: 181 KAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPL 240
           KAIGVQLSFFVYSICFPLWFLYLSYTF+FHPFQTIKLGRAYV GKL GVWELV A+VGPL
Sbjct: 184 KAIGVQLSFFVYSICFPLWFLYLSYTFIFHPFQTIKLGRAYVRGKLLGVWELVVALVGPL 243

Query: 241 ISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEV 300
           +SERFKE KSLWK G+RCVWGLLWS+YVCIIL GLLISALIFS FLMRFLVQEP+KMKEV
Sbjct: 244 VSERFKERKSLWKYGVRCVWGLLWSSYVCIILFGLLISALIFSAFLMRFLVQEPMKMKEV 303

Query: 301 LNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESE 360
           LNFDYTK SPEA MPILP+SN LYG NC++N +SGKTQ R IPPHH LQ  VSLTLPESE
Sbjct: 304 LNFDYTKPSPEALMPILPNSNDLYGHNCKDNVLSGKTQYRVIPPHHQLQAIVSLTLPESE 363

Query: 361 YNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420
           YNRNLGVFQ      VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLT+LKLAPLVTGY
Sbjct: 364 YNRNLGVFQ------VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTLLKLAPLVTGY 423

Query: 421 ISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRI 480
           ISESQTL+++LKGFTEG+IPTACLRVTIEQRAEFNPGAGIPEIY+ASLILESELPLFKRI
Sbjct: 424 ISESQTLSIKLKGFTEGNIPTACLRVTIEQRAEFNPGAGIPEIYNASLILESELPLFKRI 483

Query: 481 IWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILPRIRRRDGSANAR 522
           IWYWRKTIYVWISMTSFMMQLLF LVCCRPIILPRIRRRDGSANA+
Sbjct: 484 IWYWRKTIYVWISMTSFMMQLLFTLVCCRPIILPRIRRRDGSANAQ 522

BLAST of Spg011973 vs. NCBI nr
Match: KAG7026505.1 (Seipin-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 804.3 bits (2076), Expect = 6.9e-229
Identity = 442/534 (82.77%), Postives = 465/534 (87.08%), Query Frame = 0

Query: 1   MESHDT--KDNEDDDDFFDTLDHFPSEDSSVADQPQLSTSPPSPSD-----EISSAHAPS 60
           MESHDT  KDNEDDDDFFDTLD FPSEDSSV DQPQLSTS  S SD     E SS +  S
Sbjct: 1   MESHDTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60

Query: 61  PVNSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLE 120
            V+SLR RPSA R +AGE P+SDSSISS TSTIDDS  MSPE K PEIH +F D  KKLE
Sbjct: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGL 180
           GSE+LSVQVSS  GSSSV +EKSEVSTVTT E NS GELGVSEAESG SS+++L+LI GL
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180

Query: 181 LIKAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVG 240
           LIKAIGVQLSFFVYS CFPLWFLY SYTFVFHPFQTIKLGRAYVTGKLFG  ELV AVV 
Sbjct: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMK 300
           PLISER KEPKSLWK GLRCVWG LWSAYVCIILCGLLISALIF GFLMRFLVQEP+K+K
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDS+ LYGRNC+EN VSGK QSR IP HH+LQ  VSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVT 420
           SEYNRNLGVFQ      VRVDFLSVSGD+LASSSHPCMLQF+SEPIRLLLTILKLAPLVT
Sbjct: 361 SEYNRNLGVFQ------VRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVT 420

Query: 421 GYISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFK 480
           GYISESQTLNL+LKG  EG+IPTACLRVTIEQRAEF+PGAGIPEIYDASLILESELPLFK
Sbjct: 421 GYISESQTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFK 480

Query: 481 RIIWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILP--RIRRRDGSANARSISN 526
           RIIWYWRKT+YVWISMTSFMMQLLFMLVCCRPIILP  R+RRRDGSAN  S  N
Sbjct: 481 RIIWYWRKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRN 528

BLAST of Spg011973 vs. NCBI nr
Match: XP_008439706.1 (PREDICTED: seipin-2 [Cucumis melo] >ADN34020.1 hypothetical protein [Cucumis melo subsp. melo])

HSP 1 Score: 802.7 bits (2072), Expect = 2.0e-228
Identity = 432/525 (82.29%), Postives = 464/525 (88.38%), Query Frame = 0

Query: 1   MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLST-----SPPSPSDEISSAHAPSPV 60
           MESHDTKDNEDDDD FD+   FPSE+ S+ADQPQLST     S  SP  EISS + P+PV
Sbjct: 4   MESHDTKDNEDDDDLFDSFHDFPSENCSLADQPQLSTSTSPSSDSSPLPEISSENVPAPV 63

Query: 61  NSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGS 120
           NSLRRRPS  R IAGE PTSDSSISS T+TIDDS+  +PE K+PEIH DF+D G KLEG 
Sbjct: 64  NSLRRRPSVRRRIAGETPTSDSSISSLTTTIDDSVK-NPERKSPEIHWDFNDDGNKLEGP 123

Query: 121 ESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLI 180
           ESLSVQV+S  GSSSVNE+ +E STVTTAEINS+ ELG SE ES DSS+SILVLIAGLLI
Sbjct: 124 ESLSVQVNSWAGSSSVNEKDTEASTVTTAEINSEVELGASEVESRDSSSSILVLIAGLLI 183

Query: 181 KAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPL 240
           KAIGVQLSFFVYSICFPLWFLYLSYTF+FHPFQTIKLGRAYV GKL GVWELV A+VGPL
Sbjct: 184 KAIGVQLSFFVYSICFPLWFLYLSYTFIFHPFQTIKLGRAYVRGKLLGVWELVVALVGPL 243

Query: 241 ISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEV 300
           +SER KE KSLWK G+RCVWGLLWS+YVCIIL GLLISALIFS FLMRFLVQEP+KMKEV
Sbjct: 244 VSERLKERKSLWKYGVRCVWGLLWSSYVCIILFGLLISALIFSAFLMRFLVQEPMKMKEV 303

Query: 301 LNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESE 360
           LNFDYTK SPEA MPILP+SN LYG NC++N +SGKTQ R IPPHH LQ  VSLTLPESE
Sbjct: 304 LNFDYTKPSPEALMPILPNSNDLYGHNCKDNVLSGKTQYRVIPPHHQLQAIVSLTLPESE 363

Query: 361 YNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420
           YNRNLGVFQ      VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLT+LKLAPLVTGY
Sbjct: 364 YNRNLGVFQ------VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTLLKLAPLVTGY 423

Query: 421 ISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRI 480
           ISESQTL+++LKGFTEG+IPTACLRVTIEQRAEFNPGAGIPEIY+ASLILESELPLFKRI
Sbjct: 424 ISESQTLSIKLKGFTEGNIPTACLRVTIEQRAEFNPGAGIPEIYNASLILESELPLFKRI 483

Query: 481 IWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILPRIRRRDGSANA 521
           IWYWRKTIYVWISMTSFMMQLLF LVCCRP+ILPRIRRRDGSANA
Sbjct: 484 IWYWRKTIYVWISMTSFMMQLLFTLVCCRPLILPRIRRRDGSANA 521

BLAST of Spg011973 vs. ExPASy Swiss-Prot
Match: F4I340 (Seipin-2 OS=Arabidopsis thaliana OX=3702 GN=SEI2 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 6.1e-95
Identity = 221/496 (44.56%), Postives = 306/496 (61.69%), Query Frame = 0

Query: 47  SSAHAPSPVNSLRRRPSASRG--IAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRD 106
           S++H PS +  LRRR SA R   I+ +  T  SS S      D     S   KN E+  D
Sbjct: 36  SNSHQPSSL--LRRRKSAHRRDLISSDIETEPSSSS---DGFDVGEKSSYVEKNAELRGD 95

Query: 107 FD--DGGKKLEGSESLSVQVSS--SVGSSSVN---------------EEKSEVSTVTTAE 166
            D  D  +  + S  LS +  +   V SSS N               +E+S V+TV++ +
Sbjct: 96  IDTSDVIESTKDSIDLSSEKENDLDVISSSGNDMDVIDSGRNRVDPFQEESTVTTVSSDD 155

Query: 167 INSDGELG-VSEAESGDSST--SILVLIAGLLIKAIGVQLSFFVYSICFPLWFLYLSYTF 226
              D   G V +     +ST  S+L  + GL+IKAI  Q+SF    + FP W L   + F
Sbjct: 156 QGDDDYAGSVPQFREPPNSTEWSLLGFLVGLVIKAIEFQVSFMTSLLTFPPWLLRNCFLF 215

Query: 227 VFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPLISERFKEPKSLWKVGLRCVWGLLWSAY 286
            F PF TI+ GR ++  ++ G+ +++F  + P    R K+ K +  +  +  WG+ W+ Y
Sbjct: 216 FFDPFSTIRFGRRFLMARVAGISDMIFGYMNPF---RLKDTKQMLSIVCKFGWGMFWAVY 275

Query: 287 VCIILCGLLISALIFSGFLMRFLVQEPLKMKEVLNFDYTKHSPEAYMPILPDSNYLYGRN 346
           V I+L GLL+S+L+  G+++  +  +P ++KE LNFDYTK+SPEAY+PI   +      +
Sbjct: 276 VGIVLFGLLVSSLMIGGYVINRIADKPFEVKETLNFDYTKNSPEAYVPISSCAGVECEGS 335

Query: 347 CQENAVSGKTQS-RAIPPHHNLQVTVSLTLPESEYNRNLGVFQDDMGTVVRVDFLSVSGD 406
           C+E+    K +  R IP    L + +S+TLPES YN+NLG+FQ      VRVDFLSV G 
Sbjct: 336 CKESNEMSKIRGLRVIPRDQKLDIILSMTLPESAYNKNLGMFQ------VRVDFLSVDGQ 395

Query: 407 ILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISESQTLNLELKGFTEGDIPTACLRV 466
            +AS   PCML+F+SEPIRL+ T  K+ PLVTGY+SE QTL+L+LKGF E DIPTACL++
Sbjct: 396 TIASIRRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGFVEKDIPTACLKI 455

Query: 467 TIEQRAEFNPGAGIPEIYDASLILESELPLFKRIIWYWRKTIYVWISMTSFMMQLLFMLV 518
            IEQRAEF PGAGIPE+YDASL +ES LP F++IIW WRKT++VWISM+ F+ +LLF LV
Sbjct: 456 IIEQRAEFRPGAGIPELYDASLSVESGLPFFRKIIWKWRKTLFVWISMSLFITELLFTLV 515

BLAST of Spg011973 vs. ExPASy Swiss-Prot
Match: Q8L615 (Seipin-3 OS=Arabidopsis thaliana OX=3702 GN=SEI3 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.1e-85
Identity = 232/544 (42.65%), Postives = 313/544 (57.54%), Query Frame = 0

Query: 2   ESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLSTSPPSPSDEISSAHAPSPVNSLRRR 61
           ES  +  +   D F D  D F   DS       L++SPP+               +LRRR
Sbjct: 4   ESESSSTHSSCDRFLDAEDEF-FYDSFSNHYDCLNSSPPA---------------NLRRR 63

Query: 62  PSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGSESLSVQ 121
                        +DSS SS TS+++     S  G+N E+     D  + +E    ++  
Sbjct: 64  RLPM--------DTDSSSSSSTSSLESCEKRSTVGENDELEVSLVD-FETIEIDVDVTDS 123

Query: 122 VSSSVGSSSVNEEKSEV--STVTTAEINSDGELG-------------VSEAESGDSSTSI 181
            +SS+ S S   E  EV  S   T +   + + G               E  +G   TS 
Sbjct: 124 ANSSIDSISEKGEDFEVIDSCTDTEKNMGENDSGRVDPFTVTTLNDERGEIYTGPEYTST 183

Query: 182 LVLIAGLLIKAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWE 241
              +  L+I++I  Q+S  +  I FP W +    +FVF P++T++ GR Y          
Sbjct: 184 DWSLTSLVIRSIEFQVSLMITFIRFPPWLISKCLSFVFDPYRTMRRGRRY---------- 243

Query: 242 LVFAVVGPLISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLV 301
           LV  +VG L     K+ K + ++  R  WGL  + YV I+L  LL+SA + SGF++ +L 
Sbjct: 244 LVSWIVG-LCDSGLKDDKPVLELVRRVTWGLFCAVYVGIMLFALLVSAFMISGFVITYLA 303

Query: 302 QEPLKMKEVLNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVT 361
            EPL +KE LNFDYTK SPEAY+PI   +   +G + +E+  +GK +          ++T
Sbjct: 304 HEPLVIKESLNFDYTKSSPEAYVPISSCAGVAFGLSGKESIETGKVKGL----KDRTEIT 363

Query: 362 VSLTLPESEYNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTIL 421
           VS+TLPESEYNRNLG+FQ      VRVDFLS SG +LASS  PCM++F SEPIRL+ T+L
Sbjct: 364 VSMTLPESEYNRNLGMFQ------VRVDFLSASGHVLASSRRPCMVKFSSEPIRLVQTLL 423

Query: 422 KLAPLVTGYISESQTLNLELKGFTEGD-IPTACLRVTIEQRAEFNPGAGIPEIYDASLIL 481
           K+APLVTGY+SE QTLNL+LKG  E D IPTACL++ IEQRAEF PGAGIPEIYDASL L
Sbjct: 424 KIAPLVTGYVSEIQTLNLKLKGLVEKDIIPTACLKIMIEQRAEFRPGAGIPEIYDASLFL 483

Query: 482 ESELPLFKRIIWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILP----RIRRRDGSANAR 526
           ES+LP  KRIIW WRKT++VWISM+ F+M+LLF LV  RP+I+P    R ++RDG+    
Sbjct: 484 ESKLPFLKRIIWNWRKTLFVWISMSLFIMELLFALVFFRPLIIPRTGQRTQQRDGT---H 498

BLAST of Spg011973 vs. ExPASy Swiss-Prot
Match: Q9FFD9 (Seipin-1 OS=Arabidopsis thaliana OX=3702 GN=SEI1 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 5.6e-32
Identity = 82/262 (31.30%), Postives = 148/262 (56.49%), Query Frame = 0

Query: 248 KVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEVLNFDYTKHSPEA 307
           +V  R  +G+L + +V +++   LI A++    ++   V++P+ +++ L FDYT+ +P A
Sbjct: 83  RVVRRTWFGILGACHVSMVMVLALILAVVIGVGIVSLYVEKPVVVRDRLFFDYTEENPSA 142

Query: 308 YMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESEYNRNLGVFQDDM 367
                              +   K +S ++P  H++ V++ L +PESE NR +GVFQ   
Sbjct: 143 VF-----------------SFDKKKRSFSVPVGHSVHVSLVLWMPESEINRRIGVFQ--- 202

Query: 368 GTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISESQTLNLELK 427
              ++V+ LS+ G+ +A SS PCML+F+S+PIRL  T +   PL+ G  +E+QT+ ++  
Sbjct: 203 ---LKVELLSLKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDAL 262

Query: 428 GFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRIIWYWRKTIYVWI 487
              E    T  +R T+  RA+      +P++Y+A +++ S+ P  KR+ + W+ T+ VW 
Sbjct: 263 KHQEKMPRTKAVRATLIPRAQTRT---LPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWT 318

Query: 488 SMTSFMMQLLFMLVCCRPIILP 510
           SM  ++  L  +L C RP++ P
Sbjct: 323 SMYLYVAILTALLWCFRPVLFP 318

BLAST of Spg011973 vs. ExPASy Swiss-Prot
Match: Q9Z2E9 (Seipin OS=Mus musculus OX=10090 GN=Bscl2 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 1.3e-04
Identity = 29/83 (34.94%), Postives = 51/83 (61.45%), Query Frame = 0

Query: 344 QVTVSLTLPESEYNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLL 403
           +VT+ L LPES  N++LG+F      +V V   +  G I+++SS   ML ++S+ +++L 
Sbjct: 107 RVTLELELPESPVNQDLGMF------LVTVSCYTRGGRIISTSSRSVMLHYRSQLLQVLD 166

Query: 404 TILKLAPLVTGYISESQTLNLEL 427
           T+L  + L+ G+  + Q L +EL
Sbjct: 167 TLLFSSLLLFGFAEQKQLLEVEL 183

BLAST of Spg011973 vs. ExPASy Swiss-Prot
Match: Q5FVJ6 (Seipin OS=Rattus norvegicus OX=10116 GN=Bscl2 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 2.2e-04
Identity = 28/83 (33.73%), Postives = 51/83 (61.45%), Query Frame = 0

Query: 344 QVTVSLTLPESEYNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLL 403
           +VT+ L LPES  N++LG+F      +V V   +  G I+++SS   ML ++S+ +++L 
Sbjct: 107 RVTLELELPESPVNQDLGMF------LVTVSCYTRGGRIISTSSRSVMLHYRSQLLQMLD 166

Query: 404 TILKLAPLVTGYISESQTLNLEL 427
           T++  + L+ G+  + Q L +EL
Sbjct: 167 TLVFSSLLLFGFAEQKQLLEVEL 183

BLAST of Spg011973 vs. ExPASy TrEMBL
Match: A0A6J1KN97 (seipin-2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496838 PE=4 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 5.1e-230
Identity = 440/532 (82.71%), Postives = 465/532 (87.41%), Query Frame = 0

Query: 1   MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLSTSPPSPSD-----EISSAHAPSPV 60
           MESHDTKDNEDDDDFFDTLD FPSEDSSV DQPQLSTS  S SD     E SS +  S V
Sbjct: 1   MESHDTKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSPVLETSSENEFSSV 60

Query: 61  NSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGS 120
            SLR RPS+ R +AGE P+SDSSISS TS+IDDS+ MSPE K PEIH +F D  KKLEGS
Sbjct: 61  ASLRHRPSSRRRMAGETPSSDSSISSLTSSIDDSVMMSPEWKTPEIHPNFIDDVKKLEGS 120

Query: 121 ESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLI 180
           E+LSVQVSS  GSSSV +EKSEVSTVTT E NS GELGVSEAESG SS+++L+LI GLLI
Sbjct: 121 EALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGLLI 180

Query: 181 KAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPL 240
           KAIGVQLSFFVYS CFPLWFLY SYTFVFHPFQTIKLGRAYVTGKLFG  ELV AVV PL
Sbjct: 181 KAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVSPL 240

Query: 241 ISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEV 300
           ISER KEPKSLWK GLRCVWG LWSAYVCIILCGLLISALIF GFLMRFLVQEP+K+KEV
Sbjct: 241 ISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIKEV 300

Query: 301 LNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESE 360
           LNFDYTKHSPEAYMPILPDS+ LYGRNC+EN VSGKTQSR IP HH+LQ  VSLTLPESE
Sbjct: 301 LNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKTQSRVIPHHHHLQAIVSLTLPESE 360

Query: 361 YNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420
           YNRNLGVFQ      VRVDFLSVSGD+LASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY
Sbjct: 361 YNRNLGVFQ------VRVDFLSVSGDVLASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420

Query: 421 ISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRI 480
           ISESQTLNL+LKG  EG++PTACLRVTIEQRAEF+PGAGIPEIYDASLILESELPLFKRI
Sbjct: 421 ISESQTLNLKLKGLAEGNVPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFKRI 480

Query: 481 IWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILP--RIRRRDGSANARSISN 526
           IWYWRKT+YVWISMTSFMMQLLFMLVCCRPIILP  R+RRRD SAN  S  N
Sbjct: 481 IWYWRKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDESANVSSTRN 526

BLAST of Spg011973 vs. ExPASy TrEMBL
Match: A0A5D3CN25 (Seipin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008460 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.5e-229
Identity = 433/526 (82.32%), Postives = 465/526 (88.40%), Query Frame = 0

Query: 1   MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLST-----SPPSPSDEISSAHAPSPV 60
           MESHDTKDNEDDDD FD+   FPSE+ S+ADQPQLST     S  SP  EISS + P+PV
Sbjct: 4   MESHDTKDNEDDDDLFDSFHDFPSENCSLADQPQLSTSTSPSSDSSPLPEISSENVPAPV 63

Query: 61  NSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGS 120
           NS RRRPS  R IAGE PTSDSSISS T+TIDDS+  +PE K+PEIH DF+D G KLEG 
Sbjct: 64  NSFRRRPSVRRRIAGETPTSDSSISSLTTTIDDSVK-NPERKSPEIHWDFNDDGNKLEGP 123

Query: 121 ESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLI 180
           ESLSVQV+S  GSSSVNE+ +E STVTTAEINS+ ELG SE ES DSS+SILVLIAGLLI
Sbjct: 124 ESLSVQVNSWAGSSSVNEKDTEASTVTTAEINSEVELGASEVESRDSSSSILVLIAGLLI 183

Query: 181 KAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPL 240
           KAIGVQLSFFVYSICFPLWFLYLSYTF+FHPFQTIKLGRAYV GKL GVWELV A+VGPL
Sbjct: 184 KAIGVQLSFFVYSICFPLWFLYLSYTFIFHPFQTIKLGRAYVRGKLLGVWELVVALVGPL 243

Query: 241 ISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEV 300
           +SERFKE KSLWK G+RCVWGLLWS+YVCIIL GLLISALIFS FLMRFLVQEP+KMKEV
Sbjct: 244 VSERFKERKSLWKYGVRCVWGLLWSSYVCIILFGLLISALIFSAFLMRFLVQEPMKMKEV 303

Query: 301 LNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESE 360
           LNFDYTK SPEA MPILP+SN LYG NC++N +SGKTQ R IPPHH LQ  VSLTLPESE
Sbjct: 304 LNFDYTKPSPEALMPILPNSNDLYGHNCKDNVLSGKTQYRVIPPHHQLQAIVSLTLPESE 363

Query: 361 YNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420
           YNRNLGVFQ      VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLT+LKLAPLVTGY
Sbjct: 364 YNRNLGVFQ------VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTLLKLAPLVTGY 423

Query: 421 ISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRI 480
           ISESQTL+++LKGFTEG+IPTACLRVTIEQRAEFNPGAGIPEIY+ASLILESELPLFKRI
Sbjct: 424 ISESQTLSIKLKGFTEGNIPTACLRVTIEQRAEFNPGAGIPEIYNASLILESELPLFKRI 483

Query: 481 IWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILPRIRRRDGSANAR 522
           IWYWRKTIYVWISMTSFMMQLLF LVCCRPIILPRIRRRDGSANA+
Sbjct: 484 IWYWRKTIYVWISMTSFMMQLLFTLVCCRPIILPRIRRRDGSANAQ 522

BLAST of Spg011973 vs. ExPASy TrEMBL
Match: E5GC21 (Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 9.7e-229
Identity = 432/525 (82.29%), Postives = 464/525 (88.38%), Query Frame = 0

Query: 1   MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLST-----SPPSPSDEISSAHAPSPV 60
           MESHDTKDNEDDDD FD+   FPSE+ S+ADQPQLST     S  SP  EISS + P+PV
Sbjct: 4   MESHDTKDNEDDDDLFDSFHDFPSENCSLADQPQLSTSTSPSSDSSPLPEISSENVPAPV 63

Query: 61  NSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGS 120
           NSLRRRPS  R IAGE PTSDSSISS T+TIDDS+  +PE K+PEIH DF+D G KLEG 
Sbjct: 64  NSLRRRPSVRRRIAGETPTSDSSISSLTTTIDDSVK-NPERKSPEIHWDFNDDGNKLEGP 123

Query: 121 ESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLI 180
           ESLSVQV+S  GSSSVNE+ +E STVTTAEINS+ ELG SE ES DSS+SILVLIAGLLI
Sbjct: 124 ESLSVQVNSWAGSSSVNEKDTEASTVTTAEINSEVELGASEVESRDSSSSILVLIAGLLI 183

Query: 181 KAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPL 240
           KAIGVQLSFFVYSICFPLWFLYLSYTF+FHPFQTIKLGRAYV GKL GVWELV A+VGPL
Sbjct: 184 KAIGVQLSFFVYSICFPLWFLYLSYTFIFHPFQTIKLGRAYVRGKLLGVWELVVALVGPL 243

Query: 241 ISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEV 300
           +SER KE KSLWK G+RCVWGLLWS+YVCIIL GLLISALIFS FLMRFLVQEP+KMKEV
Sbjct: 244 VSERLKERKSLWKYGVRCVWGLLWSSYVCIILFGLLISALIFSAFLMRFLVQEPMKMKEV 303

Query: 301 LNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESE 360
           LNFDYTK SPEA MPILP+SN LYG NC++N +SGKTQ R IPPHH LQ  VSLTLPESE
Sbjct: 304 LNFDYTKPSPEALMPILPNSNDLYGHNCKDNVLSGKTQYRVIPPHHQLQAIVSLTLPESE 363

Query: 361 YNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420
           YNRNLGVFQ      VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLT+LKLAPLVTGY
Sbjct: 364 YNRNLGVFQ------VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTLLKLAPLVTGY 423

Query: 421 ISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRI 480
           ISESQTL+++LKGFTEG+IPTACLRVTIEQRAEFNPGAGIPEIY+ASLILESELPLFKRI
Sbjct: 424 ISESQTLSIKLKGFTEGNIPTACLRVTIEQRAEFNPGAGIPEIYNASLILESELPLFKRI 483

Query: 481 IWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILPRIRRRDGSANA 521
           IWYWRKTIYVWISMTSFMMQLLF LVCCRP+ILPRIRRRDGSANA
Sbjct: 484 IWYWRKTIYVWISMTSFMMQLLFTLVCCRPLILPRIRRRDGSANA 521

BLAST of Spg011973 vs. ExPASy TrEMBL
Match: A0A1S3B005 (seipin-2 OS=Cucumis melo OX=3656 GN=LOC103484422 PE=4 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 9.7e-229
Identity = 432/525 (82.29%), Postives = 464/525 (88.38%), Query Frame = 0

Query: 1   MESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLST-----SPPSPSDEISSAHAPSPV 60
           MESHDTKDNEDDDD FD+   FPSE+ S+ADQPQLST     S  SP  EISS + P+PV
Sbjct: 4   MESHDTKDNEDDDDLFDSFHDFPSENCSLADQPQLSTSTSPSSDSSPLPEISSENVPAPV 63

Query: 61  NSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGS 120
           NSLRRRPS  R IAGE PTSDSSISS T+TIDDS+  +PE K+PEIH DF+D G KLEG 
Sbjct: 64  NSLRRRPSVRRRIAGETPTSDSSISSLTTTIDDSVK-NPERKSPEIHWDFNDDGNKLEGP 123

Query: 121 ESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGLLI 180
           ESLSVQV+S  GSSSVNE+ +E STVTTAEINS+ ELG SE ES DSS+SILVLIAGLLI
Sbjct: 124 ESLSVQVNSWAGSSSVNEKDTEASTVTTAEINSEVELGASEVESRDSSSSILVLIAGLLI 183

Query: 181 KAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPL 240
           KAIGVQLSFFVYSICFPLWFLYLSYTF+FHPFQTIKLGRAYV GKL GVWELV A+VGPL
Sbjct: 184 KAIGVQLSFFVYSICFPLWFLYLSYTFIFHPFQTIKLGRAYVRGKLLGVWELVVALVGPL 243

Query: 241 ISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEV 300
           +SER KE KSLWK G+RCVWGLLWS+YVCIIL GLLISALIFS FLMRFLVQEP+KMKEV
Sbjct: 244 VSERLKERKSLWKYGVRCVWGLLWSSYVCIILFGLLISALIFSAFLMRFLVQEPMKMKEV 303

Query: 301 LNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESE 360
           LNFDYTK SPEA MPILP+SN LYG NC++N +SGKTQ R IPPHH LQ  VSLTLPESE
Sbjct: 304 LNFDYTKPSPEALMPILPNSNDLYGHNCKDNVLSGKTQYRVIPPHHQLQAIVSLTLPESE 363

Query: 361 YNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGY 420
           YNRNLGVFQ      VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLT+LKLAPLVTGY
Sbjct: 364 YNRNLGVFQ------VRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTLLKLAPLVTGY 423

Query: 421 ISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRI 480
           ISESQTL+++LKGFTEG+IPTACLRVTIEQRAEFNPGAGIPEIY+ASLILESELPLFKRI
Sbjct: 424 ISESQTLSIKLKGFTEGNIPTACLRVTIEQRAEFNPGAGIPEIYNASLILESELPLFKRI 483

Query: 481 IWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILPRIRRRDGSANA 521
           IWYWRKTIYVWISMTSFMMQLLF LVCCRP+ILPRIRRRDGSANA
Sbjct: 484 IWYWRKTIYVWISMTSFMMQLLFTLVCCRPLILPRIRRRDGSANA 521

BLAST of Spg011973 vs. ExPASy TrEMBL
Match: A0A6J1EEQ2 (seipin-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433666 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.3e-228
Identity = 441/534 (82.58%), Postives = 465/534 (87.08%), Query Frame = 0

Query: 1   MESHDT--KDNEDDDDFFDTLDHFPSEDSSVADQPQLSTSPPSPSD-----EISSAHAPS 60
           MESH+T  KDNEDDDDFFDTLD FPSEDSSV DQPQLSTS  S SD     E SS +  S
Sbjct: 1   MESHNTKDKDNEDDDDFFDTLDEFPSEDSSVTDQPQLSTSLSSISDSSTVPETSSENEFS 60

Query: 61  PVNSLRRRPSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLE 120
            V+SLR RPSA R +AGE P+SDSSISS TSTIDDS  MSPE K PEIH +F D  KKLE
Sbjct: 61  SVDSLRHRPSARRRMAGETPSSDSSISSLTSTIDDSGMMSPEWKTPEIHPNFIDDVKKLE 120

Query: 121 GSESLSVQVSSSVGSSSVNEEKSEVSTVTTAEINSDGELGVSEAESGDSSTSILVLIAGL 180
           GSE+LSVQVSS  GSSSV +EKSEVSTVTT E NS GELGVSEAESG SS+++L+LI GL
Sbjct: 121 GSEALSVQVSSWGGSSSVKDEKSEVSTVTTVETNSGGELGVSEAESGHSSSNLLMLIVGL 180

Query: 181 LIKAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWELVFAVVG 240
           LIKAIGVQLSFFVYS CFPLWFLY SYTFVFHPFQTIKLGRAYVTGKLFG  ELV AVV 
Sbjct: 181 LIKAIGVQLSFFVYSFCFPLWFLYHSYTFVFHPFQTIKLGRAYVTGKLFGFCELVTAVVS 240

Query: 241 PLISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMK 300
           PLISER KEPKSLWK GLRCVWG LWSAYVCIILCGLLISALIF GFLMRFLVQEP+K+K
Sbjct: 241 PLISERLKEPKSLWKAGLRCVWGFLWSAYVCIILCGLLISALIFGGFLMRFLVQEPVKIK 300

Query: 301 EVLNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPE 360
           EVLNFDYTKHSPEAYMPILPDS+ LYGRNC+EN VSGK QSR IP HH+LQ  VSLTLPE
Sbjct: 301 EVLNFDYTKHSPEAYMPILPDSDVLYGRNCKENVVSGKIQSRVIPHHHHLQAIVSLTLPE 360

Query: 361 SEYNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVT 420
           SEYNRNLGVFQ      VRVDFLSVSGD+LASSSHPCMLQF+SEPIRLLLTILKLAPLVT
Sbjct: 361 SEYNRNLGVFQ------VRVDFLSVSGDVLASSSHPCMLQFRSEPIRLLLTILKLAPLVT 420

Query: 421 GYISESQTLNLELKGFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFK 480
           GYISESQTLNL+LKG  EG+IPTACLRVTIEQRAEF+PGAGIPEIYDASLILESELPLFK
Sbjct: 421 GYISESQTLNLKLKGLAEGNIPTACLRVTIEQRAEFSPGAGIPEIYDASLILESELPLFK 480

Query: 481 RIIWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILP--RIRRRDGSANARSISN 526
           RIIWYWRKT+YVWISMTSFMMQLLFMLVCCRPIILP  R+RRRDGSAN  S  N
Sbjct: 481 RIIWYWRKTLYVWISMTSFMMQLLFMLVCCRPIILPRMRMRRRDGSANVSSTRN 528

BLAST of Spg011973 vs. TAIR 10
Match: AT1G29760.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 349.7 bits (896), Expect = 4.3e-96
Identity = 221/496 (44.56%), Postives = 306/496 (61.69%), Query Frame = 0

Query: 47  SSAHAPSPVNSLRRRPSASRG--IAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRD 106
           S++H PS +  LRRR SA R   I+ +  T  SS S      D     S   KN E+  D
Sbjct: 36  SNSHQPSSL--LRRRKSAHRRDLISSDIETEPSSSS---DGFDVGEKSSYVEKNAELRGD 95

Query: 107 FD--DGGKKLEGSESLSVQVSS--SVGSSSVN---------------EEKSEVSTVTTAE 166
            D  D  +  + S  LS +  +   V SSS N               +E+S V+TV++ +
Sbjct: 96  IDTSDVIESTKDSIDLSSEKENDLDVISSSGNDMDVIDSGRNRVDPFQEESTVTTVSSDD 155

Query: 167 INSDGELG-VSEAESGDSST--SILVLIAGLLIKAIGVQLSFFVYSICFPLWFLYLSYTF 226
              D   G V +     +ST  S+L  + GL+IKAI  Q+SF    + FP W L   + F
Sbjct: 156 QGDDDYAGSVPQFREPPNSTEWSLLGFLVGLVIKAIEFQVSFMTSLLTFPPWLLRNCFLF 215

Query: 227 VFHPFQTIKLGRAYVTGKLFGVWELVFAVVGPLISERFKEPKSLWKVGLRCVWGLLWSAY 286
            F PF TI+ GR ++  ++ G+ +++F  + P    R K+ K +  +  +  WG+ W+ Y
Sbjct: 216 FFDPFSTIRFGRRFLMARVAGISDMIFGYMNPF---RLKDTKQMLSIVCKFGWGMFWAVY 275

Query: 287 VCIILCGLLISALIFSGFLMRFLVQEPLKMKEVLNFDYTKHSPEAYMPILPDSNYLYGRN 346
           V I+L GLL+S+L+  G+++  +  +P ++KE LNFDYTK+SPEAY+PI   +      +
Sbjct: 276 VGIVLFGLLVSSLMIGGYVINRIADKPFEVKETLNFDYTKNSPEAYVPISSCAGVECEGS 335

Query: 347 CQENAVSGKTQS-RAIPPHHNLQVTVSLTLPESEYNRNLGVFQDDMGTVVRVDFLSVSGD 406
           C+E+    K +  R IP    L + +S+TLPES YN+NLG+FQ      VRVDFLSV G 
Sbjct: 336 CKESNEMSKIRGLRVIPRDQKLDIILSMTLPESAYNKNLGMFQ------VRVDFLSVDGQ 395

Query: 407 ILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISESQTLNLELKGFTEGDIPTACLRV 466
            +AS   PCML+F+SEPIRL+ T  K+ PLVTGY+SE QTL+L+LKGF E DIPTACL++
Sbjct: 396 TIASIRRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGFVEKDIPTACLKI 455

Query: 467 TIEQRAEFNPGAGIPEIYDASLILESELPLFKRIIWYWRKTIYVWISMTSFMMQLLFMLV 518
            IEQRAEF PGAGIPE+YDASL +ES LP F++IIW WRKT++VWISM+ F+ +LLF LV
Sbjct: 456 IIEQRAEFRPGAGIPELYDASLSVESGLPFFRKIIWKWRKTLFVWISMSLFITELLFTLV 515

BLAST of Spg011973 vs. TAIR 10
Match: AT2G34380.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 318.9 bits (816), Expect = 8.2e-87
Identity = 232/544 (42.65%), Postives = 313/544 (57.54%), Query Frame = 0

Query: 2   ESHDTKDNEDDDDFFDTLDHFPSEDSSVADQPQLSTSPPSPSDEISSAHAPSPVNSLRRR 61
           ES  +  +   D F D  D F   DS       L++SPP+               +LRRR
Sbjct: 4   ESESSSTHSSCDRFLDAEDEF-FYDSFSNHYDCLNSSPPA---------------NLRRR 63

Query: 62  PSASRGIAGENPTSDSSISSPTSTIDDSMNMSPEGKNPEIHRDFDDGGKKLEGSESLSVQ 121
                        +DSS SS TS+++     S  G+N E+     D  + +E    ++  
Sbjct: 64  RLPM--------DTDSSSSSSTSSLESCEKRSTVGENDELEVSLVD-FETIEIDVDVTDS 123

Query: 122 VSSSVGSSSVNEEKSEV--STVTTAEINSDGELG-------------VSEAESGDSSTSI 181
            +SS+ S S   E  EV  S   T +   + + G               E  +G   TS 
Sbjct: 124 ANSSIDSISEKGEDFEVIDSCTDTEKNMGENDSGRVDPFTVTTLNDERGEIYTGPEYTST 183

Query: 182 LVLIAGLLIKAIGVQLSFFVYSICFPLWFLYLSYTFVFHPFQTIKLGRAYVTGKLFGVWE 241
              +  L+I++I  Q+S  +  I FP W +    +FVF P++T++ GR Y          
Sbjct: 184 DWSLTSLVIRSIEFQVSLMITFIRFPPWLISKCLSFVFDPYRTMRRGRRY---------- 243

Query: 242 LVFAVVGPLISERFKEPKSLWKVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLV 301
           LV  +VG L     K+ K + ++  R  WGL  + YV I+L  LL+SA + SGF++ +L 
Sbjct: 244 LVSWIVG-LCDSGLKDDKPVLELVRRVTWGLFCAVYVGIMLFALLVSAFMISGFVITYLA 303

Query: 302 QEPLKMKEVLNFDYTKHSPEAYMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVT 361
            EPL +KE LNFDYTK SPEAY+PI   +   +G + +E+  +GK +          ++T
Sbjct: 304 HEPLVIKESLNFDYTKSSPEAYVPISSCAGVAFGLSGKESIETGKVKGL----KDRTEIT 363

Query: 362 VSLTLPESEYNRNLGVFQDDMGTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTIL 421
           VS+TLPESEYNRNLG+FQ      VRVDFLS SG +LASS  PCM++F SEPIRL+ T+L
Sbjct: 364 VSMTLPESEYNRNLGMFQ------VRVDFLSASGHVLASSRRPCMVKFSSEPIRLVQTLL 423

Query: 422 KLAPLVTGYISESQTLNLELKGFTEGD-IPTACLRVTIEQRAEFNPGAGIPEIYDASLIL 481
           K+APLVTGY+SE QTLNL+LKG  E D IPTACL++ IEQRAEF PGAGIPEIYDASL L
Sbjct: 424 KIAPLVTGYVSEIQTLNLKLKGLVEKDIIPTACLKIMIEQRAEFRPGAGIPEIYDASLFL 483

Query: 482 ESELPLFKRIIWYWRKTIYVWISMTSFMMQLLFMLVCCRPIILP----RIRRRDGSANAR 526
           ES+LP  KRIIW WRKT++VWISM+ F+M+LLF LV  RP+I+P    R ++RDG+    
Sbjct: 484 ESKLPFLKRIIWNWRKTLFVWISMSLFIMELLFALVFFRPLIIPRTGQRTQQRDGT---H 498

BLAST of Spg011973 vs. TAIR 10
Match: AT5G16460.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 140.6 bits (353), Expect = 4.0e-33
Identity = 82/262 (31.30%), Postives = 148/262 (56.49%), Query Frame = 0

Query: 248 KVGLRCVWGLLWSAYVCIILCGLLISALIFSGFLMRFLVQEPLKMKEVLNFDYTKHSPEA 307
           +V  R  +G+L + +V +++   LI A++    ++   V++P+ +++ L FDYT+ +P A
Sbjct: 83  RVVRRTWFGILGACHVSMVMVLALILAVVIGVGIVSLYVEKPVVVRDRLFFDYTEENPSA 142

Query: 308 YMPILPDSNYLYGRNCQENAVSGKTQSRAIPPHHNLQVTVSLTLPESEYNRNLGVFQDDM 367
                              +   K +S ++P  H++ V++ L +PESE NR +GVFQ   
Sbjct: 143 VF-----------------SFDKKKRSFSVPVGHSVHVSLVLWMPESEINRRIGVFQ--- 202

Query: 368 GTVVRVDFLSVSGDILASSSHPCMLQFKSEPIRLLLTILKLAPLVTGYISESQTLNLELK 427
              ++V+ LS+ G+ +A SS PCML+F+S+PIRL  T +   PL+ G  +E+QT+ ++  
Sbjct: 203 ---LKVELLSLKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDAL 262

Query: 428 GFTEGDIPTACLRVTIEQRAEFNPGAGIPEIYDASLILESELPLFKRIIWYWRKTIYVWI 487
              E    T  +R T+  RA+      +P++Y+A +++ S+ P  KR+ + W+ T+ VW 
Sbjct: 263 KHQEKMPRTKAVRATLIPRAQTRT---LPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWT 318

Query: 488 SMTSFMMQLLFMLVCCRPIILP 510
           SM  ++  L  +L C RP++ P
Sbjct: 323 SMYLYVAILTALLWCFRPVLFP 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883173.16.4e-23584.97seipin-2-like [Benincasa hispida][more]
XP_023003136.11.1e-22982.71seipin-2-like isoform X1 [Cucurbita maxima][more]
KAA0052594.15.3e-22982.32seipin-2 [Cucumis melo var. makuwa] >TYK13231.1 seipin-2 [Cucumis melo var. maku... [more]
KAG7026505.16.9e-22982.77Seipin-2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_008439706.12.0e-22882.29PREDICTED: seipin-2 [Cucumis melo] >ADN34020.1 hypothetical protein [Cucumis mel... [more]
Match NameE-valueIdentityDescription
F4I3406.1e-9544.56Seipin-2 OS=Arabidopsis thaliana OX=3702 GN=SEI2 PE=2 SV=1[more]
Q8L6151.1e-8542.65Seipin-3 OS=Arabidopsis thaliana OX=3702 GN=SEI3 PE=2 SV=1[more]
Q9FFD95.6e-3231.30Seipin-1 OS=Arabidopsis thaliana OX=3702 GN=SEI1 PE=2 SV=1[more]
Q9Z2E91.3e-0434.94Seipin OS=Mus musculus OX=10090 GN=Bscl2 PE=1 SV=2[more]
Q5FVJ62.2e-0433.73Seipin OS=Rattus norvegicus OX=10116 GN=Bscl2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KN975.1e-23082.71seipin-2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496838 PE=4 SV=1[more]
A0A5D3CN252.5e-22982.32Seipin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008460 PE=4... [more]
E5GC219.7e-22982.29Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3B0059.7e-22982.29seipin-2 OS=Cucumis melo OX=3656 GN=LOC103484422 PE=4 SV=1[more]
A0A6J1EEQ21.3e-22882.58seipin-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433666 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G29760.14.3e-9644.56Putative adipose-regulatory protein (Seipin) [more]
AT2G34380.18.2e-8742.65Putative adipose-regulatory protein (Seipin) [more]
AT5G16460.14.0e-3331.30Putative adipose-regulatory protein (Seipin) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009617Seipin familyPFAMPF06775Seipincoord: 270..491
e-value: 9.8E-38
score: 130.1
IPR009617Seipin familyPANTHERPTHR21212BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEINcoord: 2..525
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 116..143
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..92
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..143
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..60
NoneNo IPR availablePANTHERPTHR21212:SF4PROTEIN (SEIPIN), PUTATIVE-RELATEDcoord: 2..525

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg011973.1Spg011973.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0140042 lipid droplet formation
biological_process GO:0006629 lipid metabolic process
biological_process GO:0019915 lipid storage
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane