Spg011760 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg011760
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAspergillus nuclease S(1)
Locationscaffold1: 3349103 .. 3353572 (-)
RNA-Seq ExpressionSpg011760
SyntenySpg011760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACGTTCGTTACCTTTGATTGGGTGAGAGGCAAGCATTGAAGACCTGGAAGCAAAGCCGGTTTAATTTGGCAAACGAGCTTCAGAGCCGACGGTCAAGTAGCTGCAATCACTGCGAAGAGTGGCTTTCTGATTTAATTATCTTCATCAGCGTATTTCAGTGCTCTAAGAGCAGACATGGGCCGGTCTGAGCTTTGCTGGATCGCGATTGCTTTCGTTTTTCTGCTACTTGTACCTGAAGTCGTCGGCTGGGGAAAGGAAGGTCACTATGTAGTCTGCAAGATAGCAGAGGTAAGCATATTCAAGTCATTTTTAAGCATCAGTGGCGTTTGTTTTGTCAAGCTTTCGGTGTTACAAAAGGTTAATGGGGAATATGAAATTGTGGGGGTTTTGGCTAATACAGGAAATACATGATAATAACTGATGGGGTTGTTAATTGCAAGTTCCTTTTTACTCGGATTTTGGCTTTGAATTCTTGAAAGTGTAGCTTTCGTGGTTATATTAGTGTAGGCCAGGCCAGGATTCAAGGTAAAGCAAACGTGGGAAAGGAAAGAGGGGTCTTGTGAAATTTGGTTAGCTATTAGAAAACTTTTCGACCCATTTGATTTTTTTTTCACACAAAAGCTGAAGTAAATACTTTTATCCACTACTTTAGCTTCTTAGTGAGCTAGAAATTTGTAGTGGTGGGTTGTTTTGGAGCTGATTATATCTTATATCACCTACCCTACGTTAAGATTGCAACCACTAGATCCTGTAAAATTTTTGTTGAAGCCAAATTCTGATATCACTCCCTTTGGTTTAGGGATATTTCACCGAAGATACTTTATCAATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTTGCGGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCGCTGGAGTAGCGCCTTACACTATGTTGATACGCCTGATTTCTTTTGTAATTATGATTACTCGAGTAAGTTTAAGCTTATGGTATTTTCAGTTTCTCTCGTGGTCTTTCATGCCGTTTTATTGTTTGATATAATGCTTGTTATCATAGTGTTCACCAGCTTTAAAATTCAAATTCATTGTTGAACCACAAGAAGAATACTGAGGCTGAGAAATTTGATAGTTCATGATCACATAACTGATGGATCCTCTAAAGTTATGTTATATGGCTAATGTCATCATTGTATATCTAATTCAATCAAGCTATCCAATTGTAGTGTCGATATCTTCCTAAATTATAATTTTTGTATGCATAAGAATCTAAGGTTTTGACAAAGAGAATTTCCATGTCTCATTCCTTCAAGATGACAGTGTTCACGTAAAATGAATATACTTGTTCTTCAATTCATGTGCAGTACCCTTATGTTGCCTTTCTTGCTCTGAAACATCTTATGTTGCCAATTATGAAACCATTGAAAATATGTCATTCTGAATAACGTCATATATGATGGTCATTAAAATCAATTTCCTTTATTTGAAACTTGAAAGTCCTATTTTGACAATGTTTGCTAGGGTAACGGTATTATCTGATTCCGTTCGTTCAACGAGGTATTTTTAGTTGCAATATTTAGCTCTAATAACTAATTTCCTATATTTGAAACTTGAAAGTCCTATTTTAACAATGTTTGCTAGGGTAACGGTATTATCTGCTTCATTATAGGAGACTGTCATGACACCCATAGACACGAGGGTAGATGTGTGACTGCAGCAATTTACAACTATAGTATGCAACTCGAATCAGCTTACAAAGAAATTACTTCAGAAATTAGATGTAAGCATCGTAGATGACTAACGTTGTGCCTATACATTTTCGAACAAATGTCATTTTCATTCTGATGATATTTTCAGATAACTTAACAGAGGCTCTTATGTTCTTATCCCATTTTATTGGAGATGTCCATCAGGTTTGCTCTTTCTACAGTTCTTAATTTTAAGGCACAGAGAGATTGAAAACTTGAAATGCTAGGTTCATGAAATTTGTGCAAAATTTAGTCCTCCGAATATTTTCGAAAGTTTATTTTCATTTAATGGGATATTCTCAAGTCACTCATGCTTTTCAAATAAGATTTCTTTAAGTTTGATCCGCAGGTTTATGCATTATCTACATAGATACATTATAGCCTCCGTTAATGGAAGCTTACCTCTTTCTTTTACAGCTCTAAAATTCTACAATGCTTTTTGAAATTCTTCAGTGGTGCTGGTTTCTTGTACCTATGTACATAGATCCAAATTTTAGGTCTCAACCCCCCCCCCCCCCCAAAAAAAAAAAAACAGAGAGAGAGAGAGAGAAACCAGAATCAACACTACTAATAATGTCTACTTTATTTCATTTTTCGAGAAAGAGTTGAATAATCAATGATTTTATCTCTTTGGATCTGAATTTTCCGTTATTTGGTGGCAGCCCCTGCATGTTGGTTTCCTTGGAGATCTAGGAGGTAACTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTCCATCATGTAAGTGTCACCTAGAGATTTCAACTCCCTCCTCACAAGTACCCTCCTTAATTCTCTTTTCTTCCGGACCTATAATCAGTAGACAGTAAAACCCACAAACCATCTTGAAAAGCTTCGTTATGATGGTTAAAACTACAAGGTCGGAAATCCCACGACATATTGAAAAGCTTCGTTATTCATCTGGTATTGGAATAGTTTTGTCCATAAGAAAGAAACGGTTTTGCTCCAGGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACATTCTACCATTCTAACCTTACACTAATGATTCAAGCCATTCAAAACAACATTACGGTATGCCCTCTCTCTCTACATTTATGTCCTCATGTACCTTTTATGATAGAAGTAGAACAACTTTTTTTTCTTAGTTTCTACTTTTAGTTCTTATTCCTTATGGCATCTTCTTTTTAAATTTCTGAAGGGCGAATGGTATAATGACATCTCATCTTGGAGAAATTGCACACAGAACCACACAGCTTGTCCAAACCCGTGAGTATATTATTCTTCTCCCTTATCCATGTCTGTTGAGATGCTTATGTTCAAATCATGAATTCAAACGGTTCCTCTGCTATCACATGATCATCTCTGATTAGTATACGACAACAACGACATGATGCTTGTGATAGAAACGCACTTGCCTTGTTATCTCCAAGGCTCCAAACAACGAAGTAACCTTCATTATCCTACCATAAGGTTCTAGGACTTAATGAGATTGATTGGATGCCTCTTATTTTTCAGTTATGCTTCTGAAAGTATTAGCTTGGCATGTAAATATGCGTACAAGAATGCCACACCAGGAAGCACGCTAGAAGGTATACTTTCTTTGCCTAGACAAACATAAATACACTCATATGTATGGTTTTGTTTTCACATAAATTTCTTCATCCTATGAAAATATGTGAAATCTGAGCTTTTCTAATGGAGATTAATGGAGTGAGCCATGAGCATAATGCAAGGAATGAGGCTTTTTGATACCCTGGTTAACCCAAAAAATAGGGTACACAGCTGCACAGAATTTGATTCTATATGTCAAAACCAGATAGATGCCACTTAGTTTATTGGCAATCCTTCCTACGATAAAAAATAGACATTAGCATTCCTTTCATCAAGATCAGACGAAATGTTTGCAATCTTCCAGTTTGAATATTCTACTCAGGATTTTTTAAGCCCATCTCTTTATCCACATGAATGTCTCTGATATAAGTTCACATCTGAATCTTCCTTTCTATCTTGCAATCCTTACTGGCAAAAATATCATATTTTGGTACTGCTTGATCTTCATCTGCCAGTTTATTAACATTTACCTTCAAATATGATGCAAAAATATGAATGACTAAGGATCCGTTTGGAATAACTTTCTAAGTGTTTAAAAAATTATTTTTAAGTGTAAAAAAAATGTTTTTAAACACTTAGAAAGTCATTCCAAACATATACTAATCTGTTAAAGATGGCTGCTACTAAAAAAAAAACTTCCAGAAACGACCTGTTTGCTTCTAGGCATACAGACATGCTAGAATAATTCAACTGCTCTTTCTCTGTGCTAGCTTCCTGGATGTATAGAATGAAGAGTCATTGGCTACCCAAATAAGCAAAAATTCTCATGTTTTATTGCAGACAGCTATTTTGTTAGCCGGTTGCCAGTCGTAGAGAAGAGGTTAGCACAAAGTGGCATAAGATTGGCTGCTACCCTCAACCGTATCTTTGCTTCTGATGTGAAAGTGGCTGAAGCTTGAAGACAATGAGTAGGAGAGATCAGATCCATTAACATACAATATTAAAATGGCTTAGGACATGAATTGATTTATGCTCCATTCCTATGTCTTGTATTATATTTACGCTTGCTCCAGAAGATCCCAATATGTGGGGAATCCTCGGTAAATAGAACAGTCACTGTAAACAACCATAATATTCATGAATAATATGATTCTGTTAATTCAATCTTC

mRNA sequence

ATGGGCCGGTCTGAGCTTTGCTGGATCGCGATTGCTTTCGTTTTTCTGCTACTTGTACCTGAAGTCGTCGGCTGGGGAAAGGAAGGTCACTATGTAGTCTGCAAGATAGCAGAGGCCAGGCCAGGATTCAAGGGATATTTCACCGAAGATACTTTATCAATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTTGCGGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCGCTGGAGTAGCGCCTTACACTATGTTGATACGCCTGATTTCTTTTGTAATTATGATTACTCGAGAGACTGTCATGACACCCATAGACACGAGGGTAGATGTGTGACTGCAGCAATTTACAACTATAGTATGCAACTCGAATCAGCTTACAAAGAAATTACTTCAGAAATTAGATATAACTTAACAGAGGCTCTTATGTTCTTATCCCATTTTATTGGAGATGTCCATCAGCCCCTGCATGTTGGTTTCCTTGGAGATCTAGGAGGTAACTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTCCATCATGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACATTCTACCATTCTAACCTTACACTAATGATTCAAGCCATTCAAAACAACATTACGGGCGAATGGTATAATGACATCTCATCTTGGAGAAATTGCACACAGAACCACACAGCTTGTCCAAACCCTTATGCTTCTGAAAGTATTAGCTTGGCATGTAAATATGCGTACAAGAATGCCACACCAGGAAGCACGCTAGAAGACAGCTATTTTGTTAGCCGGTTGCCAGTCGTAGAGAAGAGGTTAGCACAAAGTGGCATAAGATTGGCTGCTACCCTCAACCGTATCTTTGCTTCTGATGTGAAAGTGGCTGAAGCTTGA

Coding sequence (CDS)

ATGGGCCGGTCTGAGCTTTGCTGGATCGCGATTGCTTTCGTTTTTCTGCTACTTGTACCTGAAGTCGTCGGCTGGGGAAAGGAAGGTCACTATGTAGTCTGCAAGATAGCAGAGGCCAGGCCAGGATTCAAGGGATATTTCACCGAAGATACTTTATCAATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGTGATCTTGCGGCTGTATGCTCCTGGCCTGATGAGATTCGACGAAAGGCTCATTATCGCTGGAGTAGCGCCTTACACTATGTTGATACGCCTGATTTCTTTTGTAATTATGATTACTCGAGAGACTGTCATGACACCCATAGACACGAGGGTAGATGTGTGACTGCAGCAATTTACAACTATAGTATGCAACTCGAATCAGCTTACAAAGAAATTACTTCAGAAATTAGATATAACTTAACAGAGGCTCTTATGTTCTTATCCCATTTTATTGGAGATGTCCATCAGCCCCTGCATGTTGGTTTCCTTGGAGATCTAGGAGGTAACTCAATAACAGTCCGTTGGTACCGTAGAAAGACTAATCTCCATCATGTATGGGATAACATGATCATTGAGTCTGCCTTGAAGACATTCTACCATTCTAACCTTACACTAATGATTCAAGCCATTCAAAACAACATTACGGGCGAATGGTATAATGACATCTCATCTTGGAGAAATTGCACACAGAACCACACAGCTTGTCCAAACCCTTATGCTTCTGAAAGTATTAGCTTGGCATGTAAATATGCGTACAAGAATGCCACACCAGGAAGCACGCTAGAAGACAGCTATTTTGTTAGCCGGTTGCCAGTCGTAGAGAAGAGGTTAGCACAAAGTGGCATAAGATTGGCTGCTACCCTCAACCGTATCTTTGCTTCTGATGTGAAAGTGGCTGAAGCTTGA

Protein sequence

MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLPASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTAAIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACPNPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDVKVAEA
Homology
BLAST of Spg011760 vs. NCBI nr
Match: XP_038883252.1 (endonuclease 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 575.9 bits (1483), Expect = 2.1e-160
Identity = 272/304 (89.47%), Postives = 290/304 (95.39%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+S+LCW A AFVFLLL+P ++GWGKEGHYV+CKIAE      GYFTEDTLS VKELLP
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAE------GYFTEDTLSTVKELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSWPDEI+RKAHYRWSSALHYVDTPDFFCNY+ SRDCHDTH H+GRCVTA
Sbjct: 61  ASAEGDLAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSRDCHDTHGHKGRCVTA 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+MQLESAYKE+TSEIRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWY
Sbjct: 121 AIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNI+ EWYND+S+WRNCT NHTACP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTLNHTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESISLACKYAYKNATPGSTLEDSYF+SRLPVVEKRLAQSGIRLA+TLNRIFAS+V
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNRIFASEV 298

Query: 301 KVAE 305
           KVAE
Sbjct: 301 KVAE 298

BLAST of Spg011760 vs. NCBI nr
Match: XP_022978904.1 (endonuclease 4-like [Cucurbita maxima])

HSP 1 Score: 573.2 bits (1476), Expect = 1.4e-159
Identity = 271/304 (89.14%), Postives = 288/304 (94.74%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+SELC IA AF FLLLVP ++GWGKEGHY++CKIAE      GYFTEDTLSMVKELLP
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAE------GYFTEDTLSMVKELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSWPDEIR KAHYRWSSALHYVDTPDFFCNY+YSRDCHDTH H+GRCVTA
Sbjct: 61  ASAEGDLAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHEHKGRCVTA 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+ QLESAYKE+TSEIRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWY
Sbjct: 121 AIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQ NI+GEWYND+S+WRNC  NHTACP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESISLACKYAYKNATPGSTLEDSYF+SRLP+VEKRLAQSGIRLA+TLNRIFAS+V
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEV 298

Query: 301 KVAE 305
           KVAE
Sbjct: 301 KVAE 298

BLAST of Spg011760 vs. NCBI nr
Match: XP_023543506.1 (endonuclease 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.4 bits (1474), Expect = 2.3e-159
Identity = 271/304 (89.14%), Postives = 288/304 (94.74%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+SELC IA AF FLLLVP ++GWGKEGHY++CKIAE      GYFTEDTLSMVKELLP
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAE------GYFTEDTLSMVKELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSWPDEIR KAHYRWSSALHYVDTPDFFCNY+YSRDCHDTH H+GRCVTA
Sbjct: 61  ASAEGDLAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTA 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+ QLESAYKE+TSEIRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWY
Sbjct: 121 AIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQ NI+GEWYND+S+WRNC  NHTACP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESISLACKYAYKNATPGSTLEDSYF+SRLP+VEKRLAQSGIRLA+TLNRIFAS+V
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEV 298

Query: 301 KVAE 305
           KVAE
Sbjct: 301 KVAE 298

BLAST of Spg011760 vs. NCBI nr
Match: XP_038883248.1 (endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883250.1 endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883251.1 endonuclease 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 567.0 bits (1460), Expect = 9.8e-158
Identity = 271/310 (87.42%), Postives = 289/310 (93.23%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+S+LCW A AFVFLLL+P ++GWGKEGHYV+CKIAE      GYFTEDTLS VKELLP
Sbjct: 1   MGQSQLCWTANAFVFLLLLPGILGWGKEGHYVICKIAE------GYFTEDTLSTVKELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSR------DCHDTHRHE 120
           ASAEGDLAAVCSWPDEI+RKAHYRWSSALHYVDTPDFFCNY+ S       DCHDTH H+
Sbjct: 61  ASAEGDLAAVCSWPDEIQRKAHYRWSSALHYVDTPDFFCNYNCSSKLKLYGDCHDTHGHK 120

Query: 121 GRCVTAAIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNS 180
           GRCVTAAIYNY+MQLESAYKE+TSEIRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNS
Sbjct: 121 GRCVTAAIYNYTMQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNS 180

Query: 181 ITVRWYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQ 240
           ITVRWYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNI+ EWYND+S+WRNCT 
Sbjct: 181 ITVRWYRRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNISDEWYNDVSAWRNCTL 240

Query: 241 NHTACPNPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNR 300
           NHTACPNPYASESISLACKYAYKNATPGSTLEDSYF+SRLPVVEKRLAQSGIRLA+TLNR
Sbjct: 241 NHTACPNPYASESISLACKYAYKNATPGSTLEDSYFLSRLPVVEKRLAQSGIRLASTLNR 300

Query: 301 IFASDVKVAE 305
           IFAS+VKVAE
Sbjct: 301 IFASEVKVAE 304

BLAST of Spg011760 vs. NCBI nr
Match: XP_022950165.1 (endonuclease 4-like [Cucurbita moschata])

HSP 1 Score: 567.0 bits (1460), Expect = 9.8e-158
Identity = 268/304 (88.16%), Postives = 286/304 (94.08%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+ ELC IA AF FLLLVP ++GWGKEGHY++CKIAE      GYFTEDTLSMVKELLP
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAE------GYFTEDTLSMVKELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSWPDEIR KAHYRWSSALHYVDTPDFFCNY+YSRDCHDTH H+GRCVTA
Sbjct: 61  ASAEGDLAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTA 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+ QLESAYKE+TSEIRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWY
Sbjct: 121 AIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQ NI+GEWYND+S+WRNC  NHTACP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESI LACKYAY+NATPGSTLEDSYF+SRLP+VEKRLAQSGIRLA+TLNRIFAS+V
Sbjct: 241 NPYASESIRLACKYAYRNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEV 298

Query: 301 KVAE 305
           KVAE
Sbjct: 301 KVAE 298

BLAST of Spg011760 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 3.4e-121
Identity = 202/296 (68.24%), Postives = 241/296 (81.42%), Query Frame = 0

Query: 8   WIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLPASAEGDL 67
           W A   V   L+   + WGKEGHY VCKIAE+      YF E+T++ VK+LLP SA+GDL
Sbjct: 8   WFARVLVLTQLINGALCWGKEGHYTVCKIAES------YFEEETVAAVKKLLPKSADGDL 67

Query: 68  AAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTAAIYNYSM 127
           A+VCSWPDEI+    +RW+S LHYVDTPD+ CNY+Y RDCHDTH+++ RCVT AI+NY+M
Sbjct: 68  ASVCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTM 127

Query: 128 QLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWYRRKTNLH 187
           QL SA +   + + YNLTEALMFLSHFIGD+HQPLHVGFLGD GGN+ITVRWYRRKTNLH
Sbjct: 128 QLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLH 187

Query: 188 HVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACPNPYASES 247
           HVWDNMIIESALKT+Y+ +L LMI+A+Q N+T +W ND+  W +C  N TACPNPYASES
Sbjct: 188 HVWDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASES 247

Query: 248 ISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDVKVA 304
           I+LACKYAY+NATPG+TL D YF+SRLP+VEKRLAQ GIRLAATLNRIF+S  K A
Sbjct: 248 INLACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSSKPKHA 297

BLAST of Spg011760 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 7.4e-108
Identity = 182/297 (61.28%), Postives = 230/297 (77.44%), Query Frame = 0

Query: 8   WIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLPASAE-GD 67
           WI    V   LV   + WGK+GHY VCK+AE      G+F +DT++ VK+LLP S + G 
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAE------GFFEDDTIAAVKKLLPESVDGGG 63

Query: 68  LAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTAAIYNYS 127
           LA  CSWPDEI++ + ++W+S LHYV+TP++ CNY+Y RDCHDTH+H+  CVT AI+NY+
Sbjct: 64  LADFCSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYT 123

Query: 128 MQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWYRRKTNL 187
            QL SA +   + + YNLTEAL+FLSH++GDVHQPLH GFLGDLGGN+I V WY  K+NL
Sbjct: 124 NQLMSASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNL 183

Query: 188 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACPNPYASE 247
           HHVWDNMII+SAL+T+Y+S+L  MIQA+Q  +   W ND+ SW++C  +  ACPN YASE
Sbjct: 184 HHVWDNMIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASE 243

Query: 248 SISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDVKVA 304
           SI LACKYAY+NATPG+TL D YF+SRLPVVEKRLAQ GIRLAATLNRIF++  K+A
Sbjct: 244 SIDLACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSAKPKLA 294

BLAST of Spg011760 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.0e-104
Identity = 185/305 (60.66%), Postives = 223/305 (73.11%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG S   WI    V   LV   + WG  GHY VCKIA++      YF EDT+  VK+LLP
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQS------YFEEDTVVAVKKLLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
            SA G+LAAVCSWPDEI++   +RW+SALH+ DTPD+ CNY+YSRDC      +  CVT 
Sbjct: 61  ESANGELAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTG 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AI+NY+ QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GDLGGN I V WY
Sbjct: 121 AIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
            ++TNLH VWD+MIIESAL+T+Y+S+L  MI  +Q  +   W ND+ SW +C  N TACP
Sbjct: 181 NQETNLHRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESI LACKYAY+NAT G+TL D YFVSRLPVVEKRLAQ GIRLA TLNRIF++  
Sbjct: 241 NPYASESIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKR 294

Query: 301 KVAEA 306
           K+A A
Sbjct: 301 KLARA 294

BLAST of Spg011760 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.5e-87
Identity = 155/281 (55.16%), Postives = 193/281 (68.68%), Query Frame = 0

Query: 16  LLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLPASAEGDLAAVCSWPD 75
           L   P + GWGKEGH ++CKIA+ R        E     VKELLP SAEGDL+++C W D
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTR------LDETAAKAVKELLPESAEGDLSSLCLWAD 78

Query: 76  EIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTAAIYNYSMQLESAYKE 135
            +  K  Y WSS LHY++TPD  C+Y Y+RDC D    +GRCV  AIYNY+ QL S    
Sbjct: 79  RV--KFRYHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTA 138

Query: 136 ITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWYRRKTNLHHVWDNMII 195
            +S+ +YNLTEAL+F+SHF+GD+HQPLHV +  D GGN+I V WY RK NLHH+WD+ II
Sbjct: 139 ASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNII 198

Query: 196 ESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACPNPYASESISLACKYA 255
           E+A    Y+S L  M+ A++ NIT EW + +  W  CT+  TACP+ YASE I  AC +A
Sbjct: 199 ETAEADLYNSALEGMVDALKKNITTEWADQVKRWETCTKK-TACPDIYASEGIQAACDWA 258

Query: 256 YKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIF 297
           YK  T G TLED YF SRLP+V +RLAQ G+RLAATLNRIF
Sbjct: 259 YKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

BLAST of Spg011760 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.4e-84
Identity = 150/296 (50.68%), Postives = 199/296 (67.23%), Query Frame = 0

Query: 9   IAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLPASAEGDLA 68
           + +  + L  V  V  W KEGH + C+IA+               +V+ LLP   +GDL+
Sbjct: 13  LVLGILILCSVSSVRSWSKEGHILTCRIAQ------NLLEAGPAHVVENLLPDYVKGDLS 72

Query: 69  AVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTAAIYNYSMQ 128
           A+C WPD+IR    YRW+S LHY+DTPD  C+Y+YSRDCHD H  +  CV  AI N++ Q
Sbjct: 73  ALCVWPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQ 132

Query: 129 LESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWYRRKTNLHH 188
           L+  Y E TS+ RYN+TEAL+FLSHF+GD+HQP+HVGF  D GGN+I +RWY+ K+NLHH
Sbjct: 133 LQH-YGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHH 192

Query: 189 VWDNMIIESALKTFYHSNLTLMIQAIQNNIT-GEWYNDISSWRNCTQNHTACPNPYASES 248
           VWD  II +ALK  Y  NL L+ + ++ NIT G W++D+SSW  C  +  ACP+ YASES
Sbjct: 193 VWDREIILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTEC-NDLIACPHKYASES 252

Query: 249 ISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDVKVA 304
           I LACK+ YK    G TL + YF +RLP+V KR+ Q G+RLA  LNR+F+ D  +A
Sbjct: 253 IKLACKWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDDHAIA 300

BLAST of Spg011760 vs. ExPASy TrEMBL
Match: A0A6J1IVA4 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.6e-160
Identity = 271/304 (89.14%), Postives = 288/304 (94.74%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+SELC IA AF FLLLVP ++GWGKEGHY++CKIAE      GYFTEDTLSMVKELLP
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAE------GYFTEDTLSMVKELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSWPDEIR KAHYRWSSALHYVDTPDFFCNY+YSRDCHDTH H+GRCVTA
Sbjct: 61  ASAEGDLAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHEHKGRCVTA 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+ QLESAYKE+TSEIRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWY
Sbjct: 121 AIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQ NI+GEWYND+S+WRNC  NHTACP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESISLACKYAYKNATPGSTLEDSYF+SRLP+VEKRLAQSGIRLA+TLNRIFAS+V
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEV 298

Query: 301 KVAE 305
           KVAE
Sbjct: 301 KVAE 298

BLAST of Spg011760 vs. ExPASy TrEMBL
Match: A0A6J1GEX6 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.8e-158
Identity = 268/304 (88.16%), Postives = 286/304 (94.08%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+ ELC IA AF FLLLVP ++GWGKEGHY++CKIAE      GYFTEDTLSMVKELLP
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAE------GYFTEDTLSMVKELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSWPDEIR KAHYRWSSALHYVDTPDFFCNY+YSRDCHDTH H+GRCVTA
Sbjct: 61  ASAEGDLAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTA 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+ QLESAYKE+TSEIRYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWY
Sbjct: 121 AIYNYTRQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQ NI+GEWYND+S+WRNC  NHTACP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQTNISGEWYNDVSAWRNCALNHTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESI LACKYAY+NATPGSTLEDSYF+SRLP+VEKRLAQSGIRLA+TLNRIFAS+V
Sbjct: 241 NPYASESIRLACKYAYRNATPGSTLEDSYFLSRLPIVEKRLAQSGIRLASTLNRIFASEV 298

Query: 301 KVAE 305
           KVAE
Sbjct: 301 KVAE 298

BLAST of Spg011760 vs. ExPASy TrEMBL
Match: A0A6J1CLU4 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111012332 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 7.9e-153
Identity = 262/305 (85.90%), Postives = 279/305 (91.48%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MGR EL W   A VFLLLVP ++GWGKEGHY +CKIAE      GYFTED LSMVKELLP
Sbjct: 1   MGRFELFW--RASVFLLLVPGILGWGKEGHYAICKIAE------GYFTEDALSMVKELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSWPDEIRRKAHY WSS LHYVDTPDFFCNY+YSRDCHDTH H GRCV A
Sbjct: 61  ASAEGDLAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIA 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+MQLES YKE+TS++RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGNSITVRWY
Sbjct: 121 AIYNYTMQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQPLHVGFIGDLGGNSITVRWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           RRKTNLHHVWDNMIIESALKTFY+S+L LMIQAIQN+I  EWYND+SSWRNCT+NHT CP
Sbjct: 181 RRKTNLHHVWDNMIIESALKTFYNSSLALMIQAIQNSIVDEWYNDVSSWRNCTENHTVCP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESISLACKYAYKNATPGSTL+DSYF+SRLPVVEKRLAQSGIRLAATLNRI AS+V
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLDDSYFLSRLPVVEKRLAQSGIRLAATLNRIAASEV 297

Query: 301 KVAEA 306
           KVAEA
Sbjct: 301 KVAEA 297

BLAST of Spg011760 vs. ExPASy TrEMBL
Match: A0A1S3CEU3 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 3.8e-147
Identity = 249/304 (81.91%), Postives = 274/304 (90.13%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+SELCW A  FVFLLL+P ++GWG+EGHY VCKIAE      GY TED LSMV+ELLP
Sbjct: 21  MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAE------GYLTEDALSMVRELLP 80

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSW DE+R    Y WSS LH+VDTPDFFCNY+ SRDCHD+HRH+GRCVTA
Sbjct: 81  ASAEGDLAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTA 140

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+MQLESAYKEITSE+RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGN I V WY
Sbjct: 141 AIYNYTMQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWY 200

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           +R TNLHHVWD MIIESALK FYHSNL LMIQAIQ+NI+ EW+N++S+WRNCT NHTACP
Sbjct: 201 QRSTNLHHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACP 260

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESISLACKYAYKNATPGSTLEDSYF++RLPVVEKRLAQSGIRLA+TLNRIFAS+V
Sbjct: 261 NPYASESISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEV 318

Query: 301 KVAE 305
           KVAE
Sbjct: 321 KVAE 318

BLAST of Spg011760 vs. ExPASy TrEMBL
Match: A0A1S3CF84 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 3.8e-147
Identity = 249/304 (81.91%), Postives = 274/304 (90.13%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG+SELCW A  FVFLLL+P ++GWG+EGHY VCKIAE      GY TED LSMV+ELLP
Sbjct: 1   MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAE------GYLTEDALSMVRELLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
           ASAEGDLAAVCSW DE+R    Y WSS LH+VDTPDFFCNY+ SRDCHD+HRH+GRCVTA
Sbjct: 61  ASAEGDLAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTA 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AIYNY+MQLESAYKEITSE+RYNLTEALMFLSHFIGDVHQPLHVGF+GDLGGN I V WY
Sbjct: 121 AIYNYTMQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQPLHVGFVGDLGGNLIKVGWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
           +R TNLHHVWD MIIESALK FYHSNL LMIQAIQ+NI+ EW+N++S+WRNCT NHTACP
Sbjct: 181 QRSTNLHHVWDTMIIESALKRFYHSNLMLMIQAIQSNISDEWHNEVSAWRNCTGNHTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESISLACKYAYKNATPGSTLEDSYF++RLPVVEKRLAQSGIRLA+TLNRIFAS+V
Sbjct: 241 NPYASESISLACKYAYKNATPGSTLEDSYFLTRLPVVEKRLAQSGIRLASTLNRIFASEV 298

Query: 301 KVAE 305
           KVAE
Sbjct: 301 KVAE 298

BLAST of Spg011760 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 436.0 bits (1120), Expect = 2.4e-122
Identity = 202/296 (68.24%), Postives = 241/296 (81.42%), Query Frame = 0

Query: 8   WIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLPASAEGDL 67
           W A   V   L+   + WGKEGHY VCKIAE+      YF E+T++ VK+LLP SA+GDL
Sbjct: 8   WFARVLVLTQLINGALCWGKEGHYTVCKIAES------YFEEETVAAVKKLLPKSADGDL 67

Query: 68  AAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTAAIYNYSM 127
           A+VCSWPDEI+    +RW+S LHYVDTPD+ CNY+Y RDCHDTH+++ RCVT AI+NY+M
Sbjct: 68  ASVCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTM 127

Query: 128 QLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWYRRKTNLH 187
           QL SA +   + + YNLTEALMFLSHFIGD+HQPLHVGFLGD GGN+ITVRWYRRKTNLH
Sbjct: 128 QLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLH 187

Query: 188 HVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACPNPYASES 247
           HVWDNMIIESALKT+Y+ +L LMI+A+Q N+T +W ND+  W +C  N TACPNPYASES
Sbjct: 188 HVWDNMIIESALKTYYNKSLPLMIEALQANLTNDWSNDVPLWESCQLNQTACPNPYASES 247

Query: 248 ISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDVKVA 304
           I+LACKYAY+NATPG+TL D YF+SRLP+VEKRLAQ GIRLAATLNRIF+S  K A
Sbjct: 248 INLACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFSSKPKHA 297

BLAST of Spg011760 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 391.7 bits (1005), Expect = 5.3e-109
Identity = 182/297 (61.28%), Postives = 230/297 (77.44%), Query Frame = 0

Query: 8   WIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLPASAE-GD 67
           WI    V   LV   + WGK+GHY VCK+AE      G+F +DT++ VK+LLP S + G 
Sbjct: 4   WIVSVLVLTHLVHGALCWGKDGHYTVCKLAE------GFFEDDTIAAVKKLLPESVDGGG 63

Query: 68  LAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTAAIYNYS 127
           LA  CSWPDEI++ + ++W+S LHYV+TP++ CNY+Y RDCHDTH+H+  CVT AI+NY+
Sbjct: 64  LADFCSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYT 123

Query: 128 MQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWYRRKTNL 187
            QL SA +   + + YNLTEAL+FLSH++GDVHQPLH GFLGDLGGN+I V WY  K+NL
Sbjct: 124 NQLMSASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNL 183

Query: 188 HHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACPNPYASE 247
           HHVWDNMII+SAL+T+Y+S+L  MIQA+Q  +   W ND+ SW++C  +  ACPN YASE
Sbjct: 184 HHVWDNMIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASE 243

Query: 248 SISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDVKVA 304
           SI LACKYAY+NATPG+TL D YF+SRLPVVEKRLAQ GIRLAATLNRIF++  K+A
Sbjct: 244 SIDLACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFSAKPKLA 294

BLAST of Spg011760 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 381.3 bits (978), Expect = 7.1e-106
Identity = 185/305 (60.66%), Postives = 223/305 (73.11%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG S   WI    V   LV   + WG  GHY VCKIA++      YF EDT+  VK+LLP
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQS------YFEEDTVVAVKKLLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
            SA G+LAAVCSWPDEI++   +RW+SALH+ DTPD+ CNY+YSRDC      +  CVT 
Sbjct: 61  ESANGELAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTG 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AI+NY+ QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GDLGGN I V WY
Sbjct: 121 AIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
            ++TNLH VWD+MIIESAL+T+Y+S+L  MI  +Q  +   W ND+ SW +C  N TACP
Sbjct: 181 NQETNLHRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESI LACKYAY+NAT G+TL D YFVSRLPVVEKRLAQ GIRLA TLNRIF++  
Sbjct: 241 NPYASESIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKR 294

Query: 301 KVAEA 306
           K+A A
Sbjct: 301 KLARA 294

BLAST of Spg011760 vs. TAIR 10
Match: AT4G21590.2 (endonuclease 3 )

HSP 1 Score: 381.3 bits (978), Expect = 7.1e-106
Identity = 185/305 (60.66%), Postives = 223/305 (73.11%), Query Frame = 0

Query: 1   MGRSELCWIAIAFVFLLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLP 60
           MG S   WI    V   LV   + WG  GHY VCKIA++      YF EDT+  VK+LLP
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQS------YFEEDTVVAVKKLLP 60

Query: 61  ASAEGDLAAVCSWPDEIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTA 120
            SA G+LAAVCSWPDEI++   +RW+SALH+ DTPD+ CNY+YSRDC      +  CVT 
Sbjct: 61  ESANGELAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTG 120

Query: 121 AIYNYSMQLESAYKEITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWY 180
           AI+NY+ QL S  +   S + YNLTEALMFLSH++GD+HQPLH GF+GDLGGN I V WY
Sbjct: 121 AIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWY 180

Query: 181 RRKTNLHHVWDNMIIESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACP 240
            ++TNLH VWD+MIIESAL+T+Y+S+L  MI  +Q  +   W ND+ SW +C  N TACP
Sbjct: 181 NQETNLHRVWDDMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACP 240

Query: 241 NPYASESISLACKYAYKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIFASDV 300
           NPYASESI LACKYAY+NAT G+TL D YFVSRLPVVEKRLAQ GIRLA TLNRIF++  
Sbjct: 241 NPYASESIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFSAKR 294

Query: 301 KVAEA 306
           K+A A
Sbjct: 301 KLARA 294

BLAST of Spg011760 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 323.6 bits (828), Expect = 1.8e-88
Identity = 155/281 (55.16%), Postives = 193/281 (68.68%), Query Frame = 0

Query: 16  LLLVPEVVGWGKEGHYVVCKIAEARPGFKGYFTEDTLSMVKELLPASAEGDLAAVCSWPD 75
           L   P + GWGKEGH ++CKIA+ R        E     VKELLP SAEGDL+++C W D
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTR------LDETAAKAVKELLPESAEGDLSSLCLWAD 78

Query: 76  EIRRKAHYRWSSALHYVDTPDFFCNYDYSRDCHDTHRHEGRCVTAAIYNYSMQLESAYKE 135
            +  K  Y WSS LHY++TPD  C+Y Y+RDC D    +GRCV  AIYNY+ QL S    
Sbjct: 79  RV--KFRYHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTA 138

Query: 136 ITSEIRYNLTEALMFLSHFIGDVHQPLHVGFLGDLGGNSITVRWYRRKTNLHHVWDNMII 195
            +S+ +YNLTEAL+F+SHF+GD+HQPLHV +  D GGN+I V WY RK NLHH+WD+ II
Sbjct: 139 ASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNII 198

Query: 196 ESALKTFYHSNLTLMIQAIQNNITGEWYNDISSWRNCTQNHTACPNPYASESISLACKYA 255
           E+A    Y+S L  M+ A++ NIT EW + +  W  CT+  TACP+ YASE I  AC +A
Sbjct: 199 ETAEADLYNSALEGMVDALKKNITTEWADQVKRWETCTKK-TACPDIYASEGIQAACDWA 258

Query: 256 YKNATPGSTLEDSYFVSRLPVVEKRLAQSGIRLAATLNRIF 297
           YK  T G TLED YF SRLP+V +RLAQ G+RLAATLNRIF
Sbjct: 259 YKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883252.12.1e-16089.47endonuclease 4-like isoform X2 [Benincasa hispida][more]
XP_022978904.11.4e-15989.14endonuclease 4-like [Cucurbita maxima][more]
XP_023543506.12.3e-15989.14endonuclease 4-like [Cucurbita pepo subsp. pepo][more]
XP_038883248.19.8e-15887.42endonuclease 4-like isoform X1 [Benincasa hispida] >XP_038883249.1 endonuclease ... [more]
XP_022950165.19.8e-15888.16endonuclease 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4JJL03.4e-12168.24Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1[more]
F4JJL37.4e-10861.28Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1[more]
Q8LDW61.0e-10460.66Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1[more]
Q9C9G42.5e-8755.16Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1[more]
Q9SXA64.4e-8450.68Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IVA46.6e-16089.14Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111478715 PE=3 SV=1[more]
A0A6J1GEX64.8e-15888.16Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111453339 PE=3 SV=... [more]
A0A6J1CLU47.9e-15385.90Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111012332 PE=3 SV... [more]
A0A1S3CEU33.8e-14781.91Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1[more]
A0A1S3CF843.8e-14781.91Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103500060 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G21585.12.4e-12268.24endonuclease 4 [more]
AT4G21600.15.3e-10961.28endonuclease 5 [more]
AT4G21590.17.1e-10660.66endonuclease 3 [more]
AT4G21590.27.1e-10660.66endonuclease 3 [more]
AT1G68290.11.8e-8855.16endonuclease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003154S1/P1 nucleasePFAMPF02265S1-P1_nucleasecoord: 25..296
e-value: 6.2E-78
score: 262.1
IPR003154S1/P1 nucleasePANTHERPTHR33146ENDONUCLEASE 4coord: 6..303
IPR003154S1/P1 nucleaseCDDcd11010S1-P1_nucleasecoord: 25..296
e-value: 6.05511E-85
score: 253.846
IPR008947Phospholipase C/P1 nuclease domain superfamilyGENE3D1.10.575.10P1 Nucleasecoord: 25..301
e-value: 7.4E-100
score: 336.1
IPR008947Phospholipase C/P1 nuclease domain superfamilySUPERFAMILY48537Phospholipase C/P1 nucleasecoord: 25..295
NoneNo IPR availablePANTHERPTHR33146:SF23ENDONUCLEASE 5coord: 6..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg011760.1Spg011760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006308 DNA catabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004521 endoribonuclease activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000014 single-stranded DNA endodeoxyribonuclease activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds