Spg011684 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg011684
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Location	scaffold1: 413610 .. 414116 (-)
RNA-Seq Expression	Spg011684
Synteny	Spg011684

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGAGAGCCATTCGAGTCACCAAAGATGGAAACCTCCGGACCCCAACCACTGGAAGCTGAACTCAGACGCCTCATGGCTCGAGGACGAAGACGCTGGTGGGCTTGGGTGGTCCATCCGTGACTCCAACAGATCTCTAATTGGAGCCGGTTGCAGGAAGATTGCCCAGAAGGGTCCGATCAAATGGCTCGAAGCTTCGGCGATGCTGGAAGGCTTGAGGGCTCTGGTTATAATGGCGAAAGATAGAGATATCGACCCAGTGCTACCGTTGGTAGTTGAAACGGATGCCTTGGAGGTGATAAACCTGTTGAACGAAGAAGGAGAAGATCTTACCGAGATTTCCATGATGATCGATGAAATCAAAGGTGTGGCCTCTCCAGATTTCGTCTCCTCTTTCTCCAAATGCTCGCGAGCGGAGAATCAGGAAACCCATGACCTTGCATGCGCTGGCGCGCGTTTGGCGAATTTTGTTATTCTTTTGGGCGCGACTTGTACCCTTAGTTAG

mRNA sequence

atgttggagagccattcgagtcaccaaagatggaaacctccggaccccaaccactggaagctgaactcagacgcctcatggctcgaggacgaagacgctggtgggcttgggtggtccatccgtgactccaacagatctctaattggagccggttgcaggaagattgcccagaagggtccgatcaaatggctcgaagcttcggcgatgctggaaggcttgagggctctggttataatggcgaaagatagagatatcgacccagtgctaccgttggtagttgaaacggatgccttggaggtgataaacctgttgaacgaagaaggagaagatcttaccgagatttccatgatgatcgatgaaatcaaaggtgtggcctctccagatttcgtctcctctttctccaaatgctcgcgagcggagaatcaggaaacccatgaccttgcatgcgctggcgcgcgtttggcgaattttgttattcttttgggcgcgacttgtacccttagttag

Coding sequence (CDS)

ATGTTGGAGAGCCATTCGAGTCACCAAAGATGGAAACCTCCGGACCCCAACCACTGGAAGCTGAACTCAGACGCCTCATGGCTCGAGGACGAAGACGCTGGTGGGCTTGGGTGGTCCATCCGTGACTCCAACAGATCTCTAATTGGAGCCGGTTGCAGGAAGATTGCCCAGAAGGGTCCGATCAAATGGCTCGAAGCTTCGGCGATGCTGGAAGGCTTGAGGGCTCTGGTTATAATGGCGAAAGATAGAGATATCGACCCAGTGCTACCGTTGGTAGTTGAAACGGATGCCTTGGAGGTGATAAACCTGTTGAACGAAGAAGGAGAAGATCTTACCGAGATTTCCATGATGATCGATGAAATCAAAGGTGTGGCCTCTCCAGATTTCGTCTCCTCTTTCTCCAAATGCTCGCGAGCGGAGAATCAGGAAACCCATGACCTTGCATGCGCTGGCGCGCGTTTGGCGAATTTTGTTATTCTTTTGGGCGCGACTTGTACCCTTAGTTAG

Protein sequence

MLESHSSHQRWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLEASAMLEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLNEEGEDLTEISMMIDEIKGVASPDFVSSFSKCSRAENQETHDLACAGARLANFVILLGATCTLS

Homology

[Close]

BLAST of Spg011684 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 103.2 bits (256), Expect = 2.2e-18
Identity = 57/144 (39.58%), Postives = 83/144 (57.64%), Query Frame = 0


Query: 10  RWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLEASAM 69
           RWKPP  N WKLN+DA+W  D + GG+GW +RD    +I A CR I  +  I +LE  A+
Sbjct: 78  RWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAI 137

Query: 70  LEGLRALVIMAKDRDIDPV-----LPLVVETDALEVINLLNEEGEDLTEISMMIDEIKGV 129
            EGLRA+    +     P+      P+ +E+D+LE I+LL+ + +D TEI  +++EI  +
Sbjct: 138 CEGLRAI----RQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQM 197

Query: 130 ASPDFVSSFSKCSRAENQETHDLA 149
                + S    SR  N+  HDLA
Sbjct: 198 MEDMKIVSMRHISREANKVAHDLA 217

[Close]

BLAST of Spg011684 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 96.7 bits (239), Expect = 2.1e-16
Identity = 53/139 (38.13%), Postives = 80/139 (57.55%), Query Frame = 0


Query: 10  RWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLEASAM 69
           +WKPP  N WKLN++A+W  D + GG+GW +RD    +I A CR I  +  I +LE  A+
Sbjct: 78  QWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAI 137

Query: 70  LEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLNEEGEDLTEISMMIDEIKGVASPDF 129
            EGLRA+         +   P+ +E+D+LE I+LL+ + +D TEI  +++EI  +     
Sbjct: 138 CEGLRAI-------RQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDME 197

Query: 130 VSSFSKCSRAENQETHDLA 149
           + S    SR  N+  H LA
Sbjct: 198 IVSMRHISREANKVAHGLA 209

[Close]

BLAST of Spg011684 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 96.3 bits (238), Expect = 2.7e-16
Identity = 54/139 (38.85%), Postives = 81/139 (58.27%), Query Frame = 0


Query: 10  RWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLEASAM 69
           +W+PP  + W LN+DASW +    GG+GW IR  +  ++ AG R +     +K LEASA+
Sbjct: 70  KWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAI 129

Query: 70  LEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLNEEGEDLTEISMMIDEIKGVASPDF 129
           LEGLR L       ++  + PL +ETD+ EV +LLN + EDLT+   +++EI  +     
Sbjct: 130 LEGLRNLT------NLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCE 189

Query: 130 VSSFSKCSRAENQETHDLA 149
           + +F+K  R  N   H LA
Sbjct: 190 ILAFAKVGRETNGCAHSLA 202

[Close]

BLAST of Spg011684 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-11
Identity = 41/100 (41.00%), Postives = 60/100 (60.00%), Query Frame = 0


Query: 6   SSHQRWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLE 65
           ++  RWKPP  N WKLN+DA+W  D +  G+GW +RD    +I  GCR I  +  I +LE
Sbjct: 250 NTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLE 309

Query: 66  ASAMLEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLN 106
             A+ EGLRA+         +   P+ +E+D+LE I+LL+
Sbjct: 310 VMAICEGLRAI-------RQEHCRPIHLESDSLEAIHLLH 342

[Close]

BLAST of Spg011684 vs. NCBI nr
Match: XP_022154991.1 (uncharacterized protein LOC111022134 isoform X2 [Momordica charantia])

HSP 1 Score: 80.1 bits (196), Expect = 2.0e-11
Identity = 41/100 (41.00%), Postives = 60/100 (60.00%), Query Frame = 0


Query: 6   SSHQRWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLE 65
           ++  RWKPP  N WKLN+DA+W  D +  G+GW +RD    +I  GCR I  +  I +LE
Sbjct: 215 NTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLE 274

Query: 66  ASAMLEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLN 106
             A+ EGLRA+         +   P+ +E+D+LE I+LL+
Sbjct: 275 VMAICEGLRAI-------RQEHCRPIHLESDSLEAIHLLH 307

[Close]

BLAST of Spg011684 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-18
Identity = 57/144 (39.58%), Postives = 83/144 (57.64%), Query Frame = 0


Query: 10  RWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLEASAM 69
           RWKPP  N WKLN+DA+W  D + GG+GW +RD    +I A CR I  +  I +LE  A+
Sbjct: 78  RWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAI 137

Query: 70  LEGLRALVIMAKDRDIDPV-----LPLVVETDALEVINLLNEEGEDLTEISMMIDEIKGV 129
            EGLRA+    +     P+      P+ +E+D+LE I+LL+ + +D TEI  +++EI  +
Sbjct: 138 CEGLRAI----RQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQM 197

Query: 130 ASPDFVSSFSKCSRAENQETHDLA 149
                + S    SR  N+  HDLA
Sbjct: 198 MEDMKIVSMRHISREANKVAHDLA 217

[Close]

BLAST of Spg011684 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.0e-16
Identity = 53/139 (38.13%), Postives = 80/139 (57.55%), Query Frame = 0


Query: 10  RWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLEASAM 69
           +WKPP  N WKLN++A+W  D + GG+GW +RD    +I A CR I  +  I +LE  A+
Sbjct: 78  QWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAI 137

Query: 70  LEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLNEEGEDLTEISMMIDEIKGVASPDF 129
            EGLRA+         +   P+ +E+D+LE I+LL+ + +D TEI  +++EI  +     
Sbjct: 138 CEGLRAI-------RQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDME 197

Query: 130 VSSFSKCSRAENQETHDLA 149
           + S    SR  N+  H LA
Sbjct: 198 IVSMRHISREANKVAHGLA 209

[Close]

BLAST of Spg011684 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.3e-16
Identity = 54/139 (38.85%), Postives = 81/139 (58.27%), Query Frame = 0


Query: 10  RWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLEASAM 69
           +W+PP  + W LN+DASW +    GG+GW IR  +  ++ AG R +     +K LEASA+
Sbjct: 70  KWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAI 129

Query: 70  LEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLNEEGEDLTEISMMIDEIKGVASPDF 129
           LEGLR L       ++  + PL +ETD+ EV +LLN + EDLT+   +++EI  +     
Sbjct: 130 LEGLRNLT------NLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCE 189

Query: 130 VSSFSKCSRAENQETHDLA 149
           + +F+K  R  N   H LA
Sbjct: 190 ILAFAKVGRETNGCAHSLA 202

[Close]

BLAST of Spg011684 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 9.7e-12
Identity = 41/100 (41.00%), Postives = 60/100 (60.00%), Query Frame = 0


Query: 6   SSHQRWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLE 65
           ++  RWKPP  N WKLN+DA+W  D +  G+GW +RD    +I  GCR I  +  I +LE
Sbjct: 250 NTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLE 309

Query: 66  ASAMLEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLN 106
             A+ EGLRA+         +   P+ +E+D+LE I+LL+
Sbjct: 310 VMAICEGLRAI-------RQEHCRPIHLESDSLEAIHLLH 342

[Close]

BLAST of Spg011684 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 9.7e-12
Identity = 41/100 (41.00%), Postives = 60/100 (60.00%), Query Frame = 0


Query: 6   SSHQRWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLE 65
           ++  RWKPP  N WKLN+DA+W  D +  G+GW +RD    +I  GCR I  +  I +LE
Sbjct: 215 NTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLE 274

Query: 66  ASAMLEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLN 106
             A+ EGLRA+         +   P+ +E+D+LE I+LL+
Sbjct: 275 VMAICEGLRAI-------RQEHCRPIHLESDSLEAIHLLH 307

[Close]

BLAST of Spg011684 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 7.9e-06
Identity = 40/144 (27.78%), Postives = 65/144 (45.14%), Query Frame = 0


Query: 4   SHSSHQRWKPPDPNHW-KLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIK 63
           + SS  RW+PP P+ W K N+DA+W  D +  G+GW +R+              +KG +K
Sbjct: 413 NRSSCGRWRPP-PHQWVKCNTDATWNRDNERCGIGWVLRN--------------EKGEVK 472

Query: 64  WLEASAM----------LEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLNEEG---- 123
           W+ A A+          LE +R  V+       + V   + E+D+  +I +LN +     
Sbjct: 473 WMGARALPKLKSVLEAELEAMRWAVLSLSRFQYNYV---IFESDSQVLIEILNNDEIWPS 532

Query: 124 -----EDLTEISMMIDEIKGVASP 128
                +DL  +     E+K V  P
Sbjct: 533 LKPTIQDLQRLLSQFTEVKFVFIP 538

[Close]

BLAST of Spg011684 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 5.1e-05
Identity = 36/128 (28.12%), Postives = 63/128 (49.22%), Query Frame = 0


Query: 10  RWKPPDPNHW-KLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLEASA 69
           +WK P P  W K N+DA+W  +    G+GW +R+ +  ++  G R + +   +  LEA  
Sbjct: 136 QWKAP-PYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNV--LEAE- 195

Query: 70  MLEGLRALVIMAKDRDIDPVLPLVVETDALEVINLLNEEG---------EDLTEISMMID 128
            LE LR  V+     +   +   + E+DA  ++NLLN +          ED+ ++    +
Sbjct: 196 -LEALRWAVLTMSRFNYKRI---IFESDAQALVNLLNSDDFWPTLQPALEDIQQLLHHFE 255

[Close]

BLAST of Spg011684 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 5.6e-04
Identity = 22/70 (31.43%), Postives = 35/70 (50.00%), Query Frame = 0


Query: 6   SSHQRWKPPDPNHWKLNSDASWLEDEDAGGLGWSIRDSNRSLIGAGCRKIAQKGPIKWLE 65
           S + +W PP  +  K N DAS  E     GLGW +R+S  ++I  G  K   +   +  E
Sbjct: 100 SRNTKWSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAE 159

Query: 66  ASAMLEGLRA 76
            S ++  ++A
Sbjct: 160 CSTLIWAIQA 169

The following BLAST results are available for this feature:

BLAST of Spg011684 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156777.1	2.2e-18	39.58	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143535.1	2.1e-16	38.13	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022155262.1	2.7e-16	38.85	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022154990.1	2.0e-11	41.00	uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]	[more]
XP_022154991.1	2.0e-11	41.00	uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]	[more]

BLAST of Spg011684 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg011684 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DSV1	1.1e-18	39.58	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CP26	1.0e-16	38.13	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DNV9	1.3e-16	38.85	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DL64	9.7e-12	41.00	uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DQC9	9.7e-12	41.00	uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Spg011684 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	7.9e-06	27.78	Ribonuclease H-like superfamily protein	[more]
AT2G34320.1	5.1e-05	28.13	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G65005.1	5.6e-04	31.43	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 17..153 e-value: 1.9E-6 score: 30.0
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 22..148 e-value: 1.1E-12 score: 47.8
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 6..154
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 21..148 e-value: 2.91813E-14 score: 63.4872
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 17..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg011684.1	Spg011684.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity