Spg011683 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg011683 vs. NCBI nr
Match: XP_023545048.1 (anaphase-promoting complex subunit 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1334.7 bits (3453), Expect = 0.0e+00
Identity = 727/1023 (71.07%), Postives = 746/1023 (72.92%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDEAE VLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 1    METDEAEHVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGTV LHDVENGKLLRS KSH  AVVSLNWV DSQLITD
Sbjct: 61   GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSQKSHEAAVVSLNWVEDSQLITD 120

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
             NENLS+YEDRTSR+FPPAPTVPRMPGLVSGDTGFIDD+EDSFTELSNSSQQRFNILCSG
Sbjct: 121  NNENLSTYEDRTSRLFPPAPTVPRMPGLVSGDTGFIDDNEDSFTELSNSSQQRFNILCSG 180

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFGIFPIGK+NIHKLHIPL  QDA A CHLLNAEIYKVALSKDLCRLIVMC
Sbjct: 181  DKDGSICFSIFGIFPIGKINIHKLHIPL--QDAGALCHLLNAEIYKVALSKDLCRLIVMC 240

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVGDGHDPRNRQI+VQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNI ELTEVIRA
Sbjct: 241  SGELVGDGHDPRNRQISVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIWELTEVIRA 300

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFDSLSTLI+NHGLDSSPQEEFLSLLGGARTSPP+HQFLVNS
Sbjct: 301  SLSVMSKQWSDAMHTFREKFDSLSTLIINHGLDSSPQEEFLSLLGGARTSPPIHQFLVNS 360

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKAVSGAGSELQLIVLDHLQPA EIIGFRMGELL LSRWRARFQ VGLDEK
Sbjct: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAAEIIGFRMGELLGLSRWRARFQSVGLDEK 420

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            LM +A EKAG LLVQV+RFMRVLSTVLQQ SNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 421  LMYDATEKAGTLLVQVERFMRVLSTVLQQISNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            VIFLKFLYNQDPVKKLLE S NDDNIEIDS                              
Sbjct: 481  VIFLKFLYNQDPVKKLLETSANDDNIEIDS------------------------------ 540

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E+VERVRELALFGGFSDCEFLRR
Sbjct: 601  -------------------------------------ELVERVRELALFGGFSDCEFLRR 660

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLGMEFQQMES                                                 
Sbjct: 661  TLGMEFQQMES------------------------------------------------- 720

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 721  ----------------------------------------------------------SF 774

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPFSTISRKILCEDLLSLFP QSSSSCLSSSVPLSVSYYE              S
Sbjct: 781  KEAFLMPFSTISRKILCEDLLSLFPLQSSSSCLSSSVPLSVSYYE-------------DS 774

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SEAVSAD SCQQ FIDYISFKVPDDSF DIANC+GIARGFIHDQSCSNEDYSSFEAVL+S
Sbjct: 841  SEAVSADLSCQQKFIDYISFKVPDDSFTDIANCIGIARGFIHDQSCSNEDYSSFEAVLIS 774

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMM++QA++LPFVSMPRSPCL NW 
Sbjct: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMIMQADELPFVSMPRSPCLINWK 774

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            VHQLKDYVVPLQMENEKVRNIPHAV+PPLAVSASRGVAC+FAARKRALVYILEEDEDEAS
Sbjct: 961  VHQLKDYVVPLQMENEKVRNIPHAVIPPLAVSASRGVACIFAARKRALVYILEEDEDEAS 774

Query: 1021 DAE 1024
            D E
Sbjct: 1021 DGE 774

BLAST of Spg011683 vs. NCBI nr
Match: XP_022949706.1 (anaphase-promoting complex subunit 4 [Cucurbita moschata])

HSP 1 Score: 1334.3 bits (3452), Expect = 0.0e+00
Identity = 727/1023 (71.07%), Postives = 747/1023 (73.02%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDEAE VLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 1    METDEAEHVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGTV LHDVENGKLLRS+KSH  AVVSLNWV DSQLITD
Sbjct: 61   GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHEAAVVSLNWVEDSQLITD 120

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
             NENLS+YEDRTSR+FPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQ+FNILCSG
Sbjct: 121  NNENLSTYEDRTSRLFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQQFNILCSG 180

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFGIFPIGK+NIHKLHIPL  QDA A CHLLNAEIYKVALSKDLCRLIVMC
Sbjct: 181  DKDGSICFSIFGIFPIGKINIHKLHIPL--QDAGALCHLLNAEIYKVALSKDLCRLIVMC 240

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVGDGHDPRNRQI+VQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNI ELTEVIRA
Sbjct: 241  SGELVGDGHDPRNRQISVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIWELTEVIRA 300

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFDSLSTLI+NHGLDSSPQEEFLSLLGGARTSPP+HQFLVNS
Sbjct: 301  SLSVMSKQWSDAMHTFREKFDSLSTLIINHGLDSSPQEEFLSLLGGARTSPPIHQFLVNS 360

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKAVSGAGSELQLIVLDHLQPA EIIGFRMGELL LSRWRARFQ VGLDEK
Sbjct: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAAEIIGFRMGELLGLSRWRARFQSVGLDEK 420

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            LM +A EKAG LLVQV+RFMRVLSTVLQQ SNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 421  LMYDATEKAGTLLVQVERFMRVLSTVLQQISNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            VIFLKFLYNQDPVKKLLE S NDDNIEIDS                              
Sbjct: 481  VIFLKFLYNQDPVKKLLETSANDDNIEIDS------------------------------ 540

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E+VERVRELALFGGFS+CEFLRR
Sbjct: 601  -------------------------------------ELVERVRELALFGGFSECEFLRR 660

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLGMEFQQMES                                                 
Sbjct: 661  TLGMEFQQMES------------------------------------------------- 720

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 721  ----------------------------------------------------------SF 774

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPFSTISRKILCEDLLSLFP QSSSSCLSSSVPLSVSYYE              S
Sbjct: 781  KEAFLMPFSTISRKILCEDLLSLFPLQSSSSCLSSSVPLSVSYYE-------------DS 774

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SEAVSAD SCQQ FIDYISFKVPDDSF DIANC+GIARGFIHDQSCSNEDYSSFEAVL+S
Sbjct: 841  SEAVSADLSCQQKFIDYISFKVPDDSFTDIANCIGIARGFIHDQSCSNEDYSSFEAVLIS 774

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMM++QA++LPFVSMPRSPCL NW 
Sbjct: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMIMQADELPFVSMPRSPCLINWK 774

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            VHQLKDYVVPLQMENEKVRNIPHAV+PPLAVSASRGVACVFAARKRALVYILEEDEDEAS
Sbjct: 961  VHQLKDYVVPLQMENEKVRNIPHAVIPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 774

Query: 1021 DAE 1024
            D E
Sbjct: 1021 DGE 774

BLAST of Spg011683 vs. NCBI nr
Match: XP_022977972.1 (anaphase-promoting complex subunit 4 [Cucurbita maxima])

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 727/1023 (71.07%), Postives = 744/1023 (72.73%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDE E VLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 1    METDETEHVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGTV LHDVENGKLLRS+KSH  AVVSLNWV DSQLITD
Sbjct: 61   GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHEAAVVSLNWVEDSQLITD 120

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
             NENLS+YEDRTSR+FPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG
Sbjct: 121  NNENLSTYEDRTSRLFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFGIFPIGK+NIH LHIPL  QDA A CHLLNAEIYKVALSKDLCRLIVMC
Sbjct: 181  DKDGSICFSIFGIFPIGKINIHMLHIPL--QDAGALCHLLNAEIYKVALSKDLCRLIVMC 240

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVGDGHDPRNRQI+VQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNI ELTEVIRA
Sbjct: 241  SGELVGDGHDPRNRQISVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIWELTEVIRA 300

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFDSLSTLI+NHGLDSSPQEEFLSLLGGARTSPP+HQFLVNS
Sbjct: 301  SLSVMSKQWSDAMHTFREKFDSLSTLIINHGLDSSPQEEFLSLLGGARTSPPIHQFLVNS 360

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKAVSGAGSELQLIVLDHLQPA EIIGFRMGELL LSRWRARFQ VGLDEK
Sbjct: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAAEIIGFRMGELLGLSRWRARFQSVGLDEK 420

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            LM  A EKAG LLVQV+RFMRVLSTVLQQ SNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 421  LMYEATEKAGTLLVQVERFMRVLSTVLQQISNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            VIFLKFLYNQDPVKKLLE S NDDNIEIDS                              
Sbjct: 481  VIFLKFLYNQDPVKKLLETSANDDNIEIDS------------------------------ 540

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E+VERVRELALFGGFSDCEFLRR
Sbjct: 601  -------------------------------------ELVERVRELALFGGFSDCEFLRR 660

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLGMEFQQMES                                                 
Sbjct: 661  TLGMEFQQMES------------------------------------------------- 720

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 721  ----------------------------------------------------------SF 774

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPFSTISRKILCEDLLSLFP QSSSSCLSSSVPLSVSYYE              S
Sbjct: 781  KEAFLMPFSTISRKILCEDLLSLFPLQSSSSCLSSSVPLSVSYYE-------------DS 774

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SEAVSAD SCQQ FIDYISFKVPDDSF DIANC+GIARGFIHDQSCSNEDYSSFEAVL+S
Sbjct: 841  SEAVSADLSCQQKFIDYISFKVPDDSFTDIANCIGIARGFIHDQSCSNEDYSSFEAVLIS 774

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMM++QA++LPFVSMPRSPCL NW 
Sbjct: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMIMQADELPFVSMPRSPCLINWK 774

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            VHQLKDYVVPLQMENEKVRNIPHAV+PPLAVSASRGVACVFAARKRALVYILEEDEDEAS
Sbjct: 961  VHQLKDYVVPLQMENEKVRNIPHAVIPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 774

Query: 1021 DAE 1024
            D E
Sbjct: 1021 DGE 774

BLAST of Spg011683 vs. NCBI nr
Match: KAG6603721.1 (Anaphase-promoting complex subunit 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 723/1023 (70.67%), Postives = 744/1023 (72.73%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDEAE VLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 61   METDEAEHVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 120

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGTV LHDVENGKLLRS+KSH  AVVSLNWV DSQL+TD
Sbjct: 121  GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHEAAVVSLNWVEDSQLLTD 180

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
             NENLS+YEDRTSR+FPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG
Sbjct: 181  NNENLSTYEDRTSRLFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 240

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFGIFPIGK+NIHKLHIPL  QDA A CHLLNAEIYKVALSKDLCRLIVMC
Sbjct: 241  DKDGSICFSIFGIFPIGKINIHKLHIPL--QDAGALCHLLNAEIYKVALSKDLCRLIVMC 300

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVGDGHDPRNRQI+VQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNI ELTEVIRA
Sbjct: 301  SGELVGDGHDPRNRQISVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIWELTEVIRA 360

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFDSLSTLI+NHGLDSSPQEEFLSLLGGARTSPP+HQFLVNS
Sbjct: 361  SLSVMSKQWSDAMHTFREKFDSLSTLIINHGLDSSPQEEFLSLLGGARTSPPIHQFLVNS 420

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKAVSGAGSELQLIVLDHLQPA EIIGFR+GELL LSRWRARFQ VGLDEK
Sbjct: 421  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAAEIIGFRLGELLGLSRWRARFQSVGLDEK 480

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            LM +A EKAG LLVQV+RFMRVLSTVLQQ SNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 481  LMYDATEKAGTLLVQVERFMRVLSTVLQQISNFFNWLIRCIKLLMSEPSDQLLPYNSELV 540

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            VIFLKFLYNQDPVKKLLE S NDDNIEID                               
Sbjct: 541  VIFLKFLYNQDPVKKLLETSANDDNIEID------------------------------- 600

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 601  ------------------------------------------------------------ 660

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E+VERVRELALFGGFSDCEFLRR
Sbjct: 661  ------------------------------------LELVERVRELALFGGFSDCEFLRR 720

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLGMEFQQMES                                                 
Sbjct: 721  TLGMEFQQMES------------------------------------------------- 780

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 781  ----------------------------------------------------------SF 834

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPFSTISRKILCEDLLSLFP QSSSSCLSSSVPLSVSYYE              S
Sbjct: 841  KEAFLMPFSTISRKILCEDLLSLFPLQSSSSCLSSSVPLSVSYYE-------------DS 834

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SE VSAD SCQQ FIDYISFKVPDDSF DIANC+GIARGFIHDQSCS+EDYSSFEAVL+S
Sbjct: 901  SEVVSADLSCQQKFIDYISFKVPDDSFTDIANCIGIARGFIHDQSCSSEDYSSFEAVLIS 834

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMM++Q ++LPFVSMPRSPCL NW 
Sbjct: 961  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMIMQVDELPFVSMPRSPCLINWK 834

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            VHQLKDYVVPLQMENEKVRNIPHAV+PPLAVSASRGVACVFAARKRALVYILEEDEDEAS
Sbjct: 1021 VHQLKDYVVPLQMENEKVRNIPHAVIPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 834

Query: 1021 DAE 1024
            D E
Sbjct: 1081 DGE 834

BLAST of Spg011683 vs. NCBI nr
Match: XP_022142094.1 (anaphase-promoting complex subunit 4 [Momordica charantia])

HSP 1 Score: 1323.1 bits (3423), Expect = 0.0e+00
Identity = 722/1023 (70.58%), Postives = 742/1023 (72.53%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDEAERVLPFQLQFDK IASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 1    METDEAERVLPFQLQFDKSIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGT+SLHDVENGKLLRS+KSH VAVVSLNWV DSQLITD
Sbjct: 61   GRSIKSLCWRPDGKVIAVGLEDGTISLHDVENGKLLRSMKSHEVAVVSLNWVEDSQLITD 120

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
             NENLS+YEDRTSRIFPPAPTVPRM GLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG
Sbjct: 121  INENLSAYEDRTSRIFPPAPTVPRMHGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFGIFPIGK+NIHKLHIPLQVQDA AS HLLNAEIYKVALSKDLCRLIVMC
Sbjct: 181  DKDGSICFSIFGIFPIGKINIHKLHIPLQVQDAGASYHLLNAEIYKVALSKDLCRLIVMC 240

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVGDGHDPRNRQ  VQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNIGEL EVIR 
Sbjct: 241  SGELVGDGHDPRNRQTNVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIGELIEVIRV 300

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFD+LS LI+NHGLDSSPQEEFLSLLGGARTS  +HQFLVNS
Sbjct: 301  SLSVMSKQWSDAMHTFREKFDALSMLIINHGLDSSPQEEFLSLLGGARTSQSIHQFLVNS 360

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKA+S AGSELQLIVLDHLQPAVEIIGFRMGELL LSRWRARFQGVGLDE+
Sbjct: 361  LGEVGAKRVSKAISSAGSELQLIVLDHLQPAVEIIGFRMGELLGLSRWRARFQGVGLDEE 420

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            L+NNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 421  LINNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            +IFLKFLYNQDPVKKLLEASENDD+IEID                               
Sbjct: 481  IIFLKFLYNQDPVKKLLEASENDDSIEID------------------------------- 540

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E++ERVREL LFGGFSDC FLRR
Sbjct: 601  ------------------------------------LEIIERVRELTLFGGFSDCGFLRR 660

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLGMEFQQMES                                                 
Sbjct: 661  TLGMEFQQMES------------------------------------------------- 720

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 721  ----------------------------------------------------------SF 776

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYE              S
Sbjct: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYE-------------DS 776

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SEAV A QSCQQ FIDYISFK+PDDS ADIANCVGIARGF+HD SCSNEDYSSFEA+LVS
Sbjct: 841  SEAVFAPQSCQQKFIDYISFKIPDDSSADIANCVGIARGFVHDLSCSNEDYSSFEAILVS 776

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGS+CVDLSLYKDGQIVLLLNETASTSES V SYMMVVQANDLPF  M RSP LD+W 
Sbjct: 901  IPDGSKCVDLSLYKDGQIVLLLNETASTSESCVSSYMMVVQANDLPFAPMSRSPSLDDWK 776

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVF ARKRALVYILEEDEDEAS
Sbjct: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFGARKRALVYILEEDEDEAS 776

Query: 1021 DAE 1024
            DAE
Sbjct: 1021 DAE 776

BLAST of Spg011683 vs. ExPASy Swiss-Prot
Match: O65418 (Anaphase-promoting complex subunit 4 OS=Arabidopsis thaliana OX=3702 GN=APC4 PE=2 SV=2)

HSP 1 Score: 942.2 bits (2434), Expect = 4.9e-273
Identity = 521/1018 (51.18%), Postives = 623/1018 (61.20%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            M +DE E ++PFQLQFDKPI  Q+KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 4    MASDEEENIIPFQLQFDKPIPFQIKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 63

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            G+ + SLCWRPDGK IAVGLEDGT+SLHDVENGKLLR++K H VAVV LNW  D Q  TD
Sbjct: 64   GKPVTSLCWRPDGKAIAVGLEDGTISLHDVENGKLLRNLKPHDVAVVCLNWEEDGQSNTD 123

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
            ++ N S YEDRTSR FPPAP  P+MPGLV+GD+ F+DD EDS  ELSN+S ++FNILC+G
Sbjct: 124  ESGNFSVYEDRTSRFFPPAPRPPKMPGLVAGDSSFMDDGEDSLAELSNTSFRKFNILCTG 183

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            D+DG+ICFSIFGIF IGK+NIH+L +P+   D  ASC L NA IYKVALSKDLCRL+VMC
Sbjct: 184  DRDGNICFSIFGIFQIGKINIHELSLPVPHLDEHASCKLFNASIYKVALSKDLCRLVVMC 243

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            +GEL      PR  +I VQ + G+H L +DTSIF KRK ELHQVAQQASNI +LTEVIRA
Sbjct: 244  TGELKDCDIKPREEKINVQDLPGLHCLAMDTSIFWKRKYELHQVAQQASNIEDLTEVIRA 303

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVM+KQW+DAM TF EKF SLSTLI+++GL+SSPQEEFLSLLGGAR SP ++QFLVNS
Sbjct: 304  SLSVMNKQWADAMKTFHEKFHSLSTLIIDNGLESSPQEEFLSLLGGARISPALNQFLVNS 363

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVG KRV K+V G G ELQ +VLDHLQPA EIIGFR+GEL  LSRWRAR+QG+GLDE 
Sbjct: 364  LGEVGVKRVLKSVCGTGKELQQVVLDHLQPAAEIIGFRIGELRGLSRWRARYQGIGLDEM 423

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            L+N A E  G+LLVQV RFM VLS+V+QQFSNFFNWL+R IK LM EP+DQLL YNSEL+
Sbjct: 424  LLNEATENTGLLLVQVQRFMMVLSSVVQQFSNFFNWLVRSIKYLMQEPNDQLLSYNSELL 483

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            V+FLKFLY+QDPVK LLE SE  D+IEID +T                            
Sbjct: 484  VVFLKFLYDQDPVKDLLELSEAGDDIEIDLKT---------------------------- 543

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 544  ------------------------------------------------------------ 603

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                   + RV+EL  FGGFS+C+FL+R
Sbjct: 604  ---------------------------------------IGRVKELLQFGGFSECDFLQR 663

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TL  EFQ MES                                                 
Sbjct: 664  TLAKEFQHMES------------------------------------------------- 723

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 724  ----------------------------------------------------------SF 771

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            K AF MPF+TISRKI C  LL L P Q S++   +++P+S+S+Y+               
Sbjct: 784  KMAFQMPFTTISRKISCMKLLPLCPLQLSTTQTPTTIPMSLSFYK--------------- 771

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
               +S D  CQ  + DYISF+VPD++F +I+NC+GIA+G+  + +     Y+S EAVL+S
Sbjct: 844  -NELSDDTPCQSGYTDYISFQVPDETFPEISNCIGIAKGYKQNSNNEKNGYTSLEAVLLS 771

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            +P+G  CVDLSLYKD ++VLLLN+T + SE S  + MMVVQ  DL F+S+  S  L+ W 
Sbjct: 904  VPNGYTCVDLSLYKDKELVLLLNKTNTDSEGSGEACMMVVQTGDLAFISISGSSSLNQWE 771

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDE 1019
            +  LK  +V L+MENEKVR +PH+V+ PLAVSASRGVACVFA R+RALVYILEEDEDE
Sbjct: 964  LEDLKGSIVNLEMENEKVRKVPHSVIAPLAVSASRGVACVFAERRRALVYILEEDEDE 771

BLAST of Spg011683 vs. ExPASy Swiss-Prot
Match: Q5RAQ5 (Anaphase-promoting complex subunit 4 OS=Pongo abelii OX=9601 GN=ANAPC4 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 4.2e-38
Identity = 124/497 (24.95%), Postives = 235/497 (47.28%), Query Frame = 0

Query: 12  FQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRF-NWQRLWTISP----GRSIKS 71
           F++  +K +  ++    W+P++DL+A+     ++LLHR  ++ R+W+  P    G+ +  
Sbjct: 11  FRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTC 70

Query: 72  LCWRPDGKVIAVGLED-GTVSLHDVENGKLLRSVKSHAVAVVSLNWV---VDSQLITDKN 131
           L WRPDGK++A  L D   + L DVE    L S    A  V  ++W+   V+S ++T   
Sbjct: 71  LAWRPDGKLLAFALADTKKIVLCDVEKPGSLHSFSVEA-PVSCMHWMEVTVESSVLT--- 130

Query: 132 ENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDK 191
            +  + ED ++ + P  PT+P+     S    F +++ D   +L      R NIL  G  
Sbjct: 131 -SFYNAEDESNLLLPKLPTLPK--NYSSTSKIFSEENSDEIIKL--LGDVRLNILVLGGS 190

Query: 192 DGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMCSG 251
            G I    +G+F I +V              + +C  L        LS DL  L V+   
Sbjct: 191 SGFIELYAYGMFKIARVT-----------GIAGTCLAL-------CLSSDLKSLSVV--- 250

Query: 252 ELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRASL 311
                       +++  G   +    L+T++      E+ ++A++ ++I  L + I  SL
Sbjct: 251 -----------TEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSL 310

Query: 312 SVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNSLG 371
           + M + W + +     +   L+  +      +S Q+EF+ LL   + S  +   L+N L 
Sbjct: 311 TCMCEAWEEILMQMDSR---LTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLT 370

Query: 372 EVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEKLM 431
             G K++ +++  + S +Q +V+ HLQ   E + + + EL  L+ W+ +++ +GLD   +
Sbjct: 371 VKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGLASWKQKYEPLGLDAAGI 430

Query: 432 NNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSEL--- 491
             A+   G  +++ +  ++V+ + ++ F  FF WL   + L M+E  D +LP  +++   
Sbjct: 431 EEAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAM-LRMTE--DHVLPELNKMTQK 460

Query: 492 ----VVIFLKFLYNQDP 493
               V  FL   +N+ P
Sbjct: 491 DITFVAEFLTEHFNEAP 460

BLAST of Spg011683 vs. ExPASy Swiss-Prot
Match: Q9UJX5 (Anaphase-promoting complex subunit 4 OS=Homo sapiens OX=9606 GN=ANAPC4 PE=1 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 5.5e-38
Identity = 123/497 (24.75%), Postives = 235/497 (47.28%), Query Frame = 0

Query: 12  FQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRF-NWQRLWTISP----GRSIKS 71
           F++  +K +  ++    W+P++DL+A+     ++LLHR  ++ R+W+  P    G+ +  
Sbjct: 11  FRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTC 70

Query: 72  LCWRPDGKVIAVGLED-GTVSLHDVENGKLLRSVKSHAVAVVSLNWV---VDSQLITDKN 131
           L WRPDGK++A  L D   + L DVE  + L S    A  V  ++W+   V+S ++T   
Sbjct: 71  LAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEA-PVSCMHWMEVTVESSVLT--- 130

Query: 132 ENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDK 191
            +  + ED ++ + P  PT+P+       +T  I   E+S   +      R NIL  G  
Sbjct: 131 -SFYNAEDESNLLLPKLPTLPKN----YSNTSKIFSEENSDEIIKLLGDVRLNILVLGGS 190

Query: 192 DGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMCSG 251
            G I    +G+F I +V              + +C  L        LS DL  L V+   
Sbjct: 191 SGFIELYAYGMFKIARVT-----------GIAGTCLAL-------CLSSDLKSLSVV--- 250

Query: 252 ELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRASL 311
                       +++  G   +    L+T++      E+ ++A++ ++I  L + I  SL
Sbjct: 251 -----------TEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSL 310

Query: 312 SVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNSLG 371
           + M + W + +     +   L+  +      +S Q+EF+ LL   + S  +   L+N L 
Sbjct: 311 TCMCEAWEEILMQMDSR---LTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLT 370

Query: 372 EVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEKLM 431
             G K++ +++  + S +Q +V+ HLQ   E + + + EL  ++ W+ +++ +GLD   +
Sbjct: 371 VKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGI 430

Query: 432 NNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSEL--- 491
             A+   G  +++ +  ++V+ + ++ F  FF WL   + L M+E  D +LP  +++   
Sbjct: 431 EEAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAM-LRMTE--DHVLPELNKMTQK 460

Query: 492 ----VVIFLKFLYNQDP 493
               V  FL   +N+ P
Sbjct: 491 DITFVAEFLTEHFNEAP 460

BLAST of Spg011683 vs. ExPASy Swiss-Prot
Match: Q91W96 (Anaphase-promoting complex subunit 4 OS=Mus musculus OX=10090 GN=Anapc4 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 3.5e-37
Identity = 123/497 (24.75%), Postives = 234/497 (47.08%), Query Frame = 0

Query: 12  FQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRF-NWQRLWTISP----GRSIKS 71
           F++  +K +  ++    W+P++DL+A+     ++LLHR  ++ R+W+  P    G+ +  
Sbjct: 11  FRVVGEKQLPQEIIFLAWSPKRDLIALANTTGEVLLHRLASFHRVWSFPPNESTGKEVTC 70

Query: 72  LCWRPDGKVIAVGLED-GTVSLHDVENGKLLRSVKSHAVAVVSLNW---VVDSQLITDKN 131
           L WRPDGK++A  L D   + L DVE  + L S    A  V  ++W    V+S ++T   
Sbjct: 71  LAWRPDGKLLAFALADTKKIILCDVEKPESLHSFSVEA-PVSCMHWTEVTVESSVLT--- 130

Query: 132 ENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSGDK 191
            +  + ED ++ + P  PT+P+        T  I   E+S   +      R NIL  G  
Sbjct: 131 -SFYNAEDESNLLLPKLPTLPKN----YNSTSKIFSEENSDEIIKLLGDVRLNILVLGGS 190

Query: 192 DGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMCSG 251
            G I    +G+F I +V              + +C  L        LS DL  L V+   
Sbjct: 191 SGFIELYAYGMFKIARVT-----------GIAGTCIAL-------CLSSDLKSLSVV--- 250

Query: 252 ELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRASL 311
                       +++  G   +    L+T++      E+ ++A++ ++I  L + I  SL
Sbjct: 251 -----------TEVSSGGESEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSL 310

Query: 312 SVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNSLG 371
           + M + W + +     +   L+  +      +S Q+EF+ LL   + S  +   L+N L 
Sbjct: 311 TCMCEAWEEILMQMDSR---LTKFVQEKPTTTSVQDEFMHLLLWGKASAELQTLLMNQLT 370

Query: 372 EVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEKLM 431
             G K++ +++  + S +Q +V+ HLQ   E + + + EL  ++ W+ +++ +GLD   +
Sbjct: 371 VKGLKKLGQSIESSYSSIQKLVISHLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGI 430

Query: 432 NNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSEL--- 491
            +A+   G  +++ +  ++V+ + ++ F  FF WL   + L M+E  D +LP  +++   
Sbjct: 431 EDAITAVGSFILKANELLQVIDSSMKNFKAFFRWLYVAM-LRMTE--DHVLPELNKMTQK 460

Query: 492 ----VVIFLKFLYNQDP 493
               V  FL   +N+ P
Sbjct: 491 DITFVAEFLTEHFNEAP 460

BLAST of Spg011683 vs. ExPASy Swiss-Prot
Match: Q54NI1 (Anaphase-promoting complex subunit 4 OS=Dictyostelium discoideum OX=44689 GN=anapc4 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.3e-26
Identity = 117/557 (21.01%), Postives = 240/557 (43.09%), Query Frame = 0

Query: 12  FQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRF-NWQRLWTIS----------- 71
           F L  DK + + VK        DL+A+VT+D +I++HRF  WQ+L+TI+           
Sbjct: 4   FVLLNDKILPNDVKCYSCCTTMDLIALVTKDDQIVIHRFLTWQKLFTINHMSTTINDNNT 63

Query: 72  -----------------------PGRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLL 131
                                    +SI S+ W P+GK+I++G EDG++ ++++EN KL+
Sbjct: 64  NNNNNNNNNNNNNNNDNNNDNDKSNKSIVSIQWSPNGKMISIGCEDGSIFIYNIENAKLI 123

Query: 132 RSVKSHAVAVVSLNWVVD-------------------------------------SQLIT 191
               +H   +  L W+ +                                      Q   
Sbjct: 124 NKSHNHKHPIHKLAWIKEVSQQRSQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ 183

Query: 192 DKNENLSSYEDRTSRIFPP--APTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQR-FNI 251
            +N+N  S +   + + PP     + +   L    + + ++S++    L      R F+I
Sbjct: 184 QQNKN-GSTKCNNNYVSPPLFLSQIKQNMNLFPSISYYFENSKEENIYLGGDIYDRPFDI 243

Query: 252 LCSGDKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLL-----NAEIYKVALSK 311
           L   D  G I    FG+F I  +++  L     ++   ++ H L     + +I  + L++
Sbjct: 244 LICCDSIGVISLLAFGLFKIVTIDLLSL-----LKQKYSNTHFLIKPSKSLKILDITLTE 303

Query: 312 DLCRLIVMCSGELVGDGHDPRNRQITVQGVHGMH-SLVLDTSIFRKRKGELHQVAQQASN 371
            L +L VM                  ++  +G+  S+ +DTSI  +++ E+H+++ Q   
Sbjct: 304 SLNKLSVM------------------IETDNGLFLSVTIDTSILLEKRNEIHEISLQYFL 363

Query: 372 IGELTEVIRASLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTS 431
           + +L + +   +  ++++W +       K+     ++ ++G  SS ++E + LL     S
Sbjct: 364 LFQLQQSLDIHIKEITEKWKETQQQLSTKWVEFEKVLSDYGFSSSIEQELIDLLMCGVPS 423

Query: 432 PPVHQFLVNSLGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRA 486
           PP +QF+VN+   +  K++    S   S ++ I++ ++ P+   I   +  L   S    
Sbjct: 424 PPTNQFIVNN---INIKKLKLTESNCNS-IREILIKYILPSFLNIFHIITVLHNNSLEND 483

BLAST of Spg011683 vs. ExPASy TrEMBL
Match: A0A6J1GCS1 (Anaphase-promoting complex subunit 4 OS=Cucurbita moschata OX=3662 GN=LOC111453021 PE=4 SV=1)

HSP 1 Score: 1334.3 bits (3452), Expect = 0.0e+00
Identity = 727/1023 (71.07%), Postives = 747/1023 (73.02%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDEAE VLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 1    METDEAEHVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGTV LHDVENGKLLRS+KSH  AVVSLNWV DSQLITD
Sbjct: 61   GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHEAAVVSLNWVEDSQLITD 120

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
             NENLS+YEDRTSR+FPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQ+FNILCSG
Sbjct: 121  NNENLSTYEDRTSRLFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQQFNILCSG 180

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFGIFPIGK+NIHKLHIPL  QDA A CHLLNAEIYKVALSKDLCRLIVMC
Sbjct: 181  DKDGSICFSIFGIFPIGKINIHKLHIPL--QDAGALCHLLNAEIYKVALSKDLCRLIVMC 240

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVGDGHDPRNRQI+VQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNI ELTEVIRA
Sbjct: 241  SGELVGDGHDPRNRQISVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIWELTEVIRA 300

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFDSLSTLI+NHGLDSSPQEEFLSLLGGARTSPP+HQFLVNS
Sbjct: 301  SLSVMSKQWSDAMHTFREKFDSLSTLIINHGLDSSPQEEFLSLLGGARTSPPIHQFLVNS 360

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKAVSGAGSELQLIVLDHLQPA EIIGFRMGELL LSRWRARFQ VGLDEK
Sbjct: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAAEIIGFRMGELLGLSRWRARFQSVGLDEK 420

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            LM +A EKAG LLVQV+RFMRVLSTVLQQ SNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 421  LMYDATEKAGTLLVQVERFMRVLSTVLQQISNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            VIFLKFLYNQDPVKKLLE S NDDNIEIDS                              
Sbjct: 481  VIFLKFLYNQDPVKKLLETSANDDNIEIDS------------------------------ 540

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E+VERVRELALFGGFS+CEFLRR
Sbjct: 601  -------------------------------------ELVERVRELALFGGFSECEFLRR 660

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLGMEFQQMES                                                 
Sbjct: 661  TLGMEFQQMES------------------------------------------------- 720

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 721  ----------------------------------------------------------SF 774

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPFSTISRKILCEDLLSLFP QSSSSCLSSSVPLSVSYYE              S
Sbjct: 781  KEAFLMPFSTISRKILCEDLLSLFPLQSSSSCLSSSVPLSVSYYE-------------DS 774

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SEAVSAD SCQQ FIDYISFKVPDDSF DIANC+GIARGFIHDQSCSNEDYSSFEAVL+S
Sbjct: 841  SEAVSADLSCQQKFIDYISFKVPDDSFTDIANCIGIARGFIHDQSCSNEDYSSFEAVLIS 774

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMM++QA++LPFVSMPRSPCL NW 
Sbjct: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMIMQADELPFVSMPRSPCLINWK 774

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            VHQLKDYVVPLQMENEKVRNIPHAV+PPLAVSASRGVACVFAARKRALVYILEEDEDEAS
Sbjct: 961  VHQLKDYVVPLQMENEKVRNIPHAVIPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 774

Query: 1021 DAE 1024
            D E
Sbjct: 1021 DGE 774

BLAST of Spg011683 vs. ExPASy TrEMBL
Match: A0A6J1IJW1 (Anaphase-promoting complex subunit 4 OS=Cucurbita maxima OX=3661 GN=LOC111478105 PE=4 SV=1)

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 727/1023 (71.07%), Postives = 744/1023 (72.73%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDE E VLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 1    METDETEHVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGTV LHDVENGKLLRS+KSH  AVVSLNWV DSQLITD
Sbjct: 61   GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHEAAVVSLNWVEDSQLITD 120

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
             NENLS+YEDRTSR+FPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG
Sbjct: 121  NNENLSTYEDRTSRLFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFGIFPIGK+NIH LHIPL  QDA A CHLLNAEIYKVALSKDLCRLIVMC
Sbjct: 181  DKDGSICFSIFGIFPIGKINIHMLHIPL--QDAGALCHLLNAEIYKVALSKDLCRLIVMC 240

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVGDGHDPRNRQI+VQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNI ELTEVIRA
Sbjct: 241  SGELVGDGHDPRNRQISVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIWELTEVIRA 300

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFDSLSTLI+NHGLDSSPQEEFLSLLGGARTSPP+HQFLVNS
Sbjct: 301  SLSVMSKQWSDAMHTFREKFDSLSTLIINHGLDSSPQEEFLSLLGGARTSPPIHQFLVNS 360

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKAVSGAGSELQLIVLDHLQPA EIIGFRMGELL LSRWRARFQ VGLDEK
Sbjct: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAAEIIGFRMGELLGLSRWRARFQSVGLDEK 420

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            LM  A EKAG LLVQV+RFMRVLSTVLQQ SNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 421  LMYEATEKAGTLLVQVERFMRVLSTVLQQISNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            VIFLKFLYNQDPVKKLLE S NDDNIEIDS                              
Sbjct: 481  VIFLKFLYNQDPVKKLLETSANDDNIEIDS------------------------------ 540

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E+VERVRELALFGGFSDCEFLRR
Sbjct: 601  -------------------------------------ELVERVRELALFGGFSDCEFLRR 660

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLGMEFQQMES                                                 
Sbjct: 661  TLGMEFQQMES------------------------------------------------- 720

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 721  ----------------------------------------------------------SF 774

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPFSTISRKILCEDLLSLFP QSSSSCLSSSVPLSVSYYE              S
Sbjct: 781  KEAFLMPFSTISRKILCEDLLSLFPLQSSSSCLSSSVPLSVSYYE-------------DS 774

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SEAVSAD SCQQ FIDYISFKVPDDSF DIANC+GIARGFIHDQSCSNEDYSSFEAVL+S
Sbjct: 841  SEAVSADLSCQQKFIDYISFKVPDDSFTDIANCIGIARGFIHDQSCSNEDYSSFEAVLIS 774

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMM++QA++LPFVSMPRSPCL NW 
Sbjct: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMIMQADELPFVSMPRSPCLINWK 774

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            VHQLKDYVVPLQMENEKVRNIPHAV+PPLAVSASRGVACVFAARKRALVYILEEDEDEAS
Sbjct: 961  VHQLKDYVVPLQMENEKVRNIPHAVIPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 774

Query: 1021 DAE 1024
            D E
Sbjct: 1021 DGE 774

BLAST of Spg011683 vs. ExPASy TrEMBL
Match: A0A6J1CKL4 (Anaphase-promoting complex subunit 4 OS=Momordica charantia OX=3673 GN=LOC111012308 PE=4 SV=1)

HSP 1 Score: 1323.1 bits (3423), Expect = 0.0e+00
Identity = 722/1023 (70.58%), Postives = 742/1023 (72.53%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDEAERVLPFQLQFDK IASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 1    METDEAERVLPFQLQFDKSIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGT+SLHDVENGKLLRS+KSH VAVVSLNWV DSQLITD
Sbjct: 61   GRSIKSLCWRPDGKVIAVGLEDGTISLHDVENGKLLRSMKSHEVAVVSLNWVEDSQLITD 120

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
             NENLS+YEDRTSRIFPPAPTVPRM GLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG
Sbjct: 121  INENLSAYEDRTSRIFPPAPTVPRMHGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFGIFPIGK+NIHKLHIPLQVQDA AS HLLNAEIYKVALSKDLCRLIVMC
Sbjct: 181  DKDGSICFSIFGIFPIGKINIHKLHIPLQVQDAGASYHLLNAEIYKVALSKDLCRLIVMC 240

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVGDGHDPRNRQ  VQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNIGEL EVIR 
Sbjct: 241  SGELVGDGHDPRNRQTNVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIGELIEVIRV 300

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFD+LS LI+NHGLDSSPQEEFLSLLGGARTS  +HQFLVNS
Sbjct: 301  SLSVMSKQWSDAMHTFREKFDALSMLIINHGLDSSPQEEFLSLLGGARTSQSIHQFLVNS 360

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKA+S AGSELQLIVLDHLQPAVEIIGFRMGELL LSRWRARFQGVGLDE+
Sbjct: 361  LGEVGAKRVSKAISSAGSELQLIVLDHLQPAVEIIGFRMGELLGLSRWRARFQGVGLDEE 420

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            L+NNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 421  LINNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            +IFLKFLYNQDPVKKLLEASENDD+IEID                               
Sbjct: 481  IIFLKFLYNQDPVKKLLEASENDDSIEID------------------------------- 540

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E++ERVREL LFGGFSDC FLRR
Sbjct: 601  ------------------------------------LEIIERVRELTLFGGFSDCGFLRR 660

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLGMEFQQMES                                                 
Sbjct: 661  TLGMEFQQMES------------------------------------------------- 720

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 721  ----------------------------------------------------------SF 776

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYE              S
Sbjct: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYE-------------DS 776

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SEAV A QSCQQ FIDYISFK+PDDS ADIANCVGIARGF+HD SCSNEDYSSFEA+LVS
Sbjct: 841  SEAVFAPQSCQQKFIDYISFKIPDDSSADIANCVGIARGFVHDLSCSNEDYSSFEAILVS 776

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGS+CVDLSLYKDGQIVLLLNETASTSES V SYMMVVQANDLPF  M RSP LD+W 
Sbjct: 901  IPDGSKCVDLSLYKDGQIVLLLNETASTSESCVSSYMMVVQANDLPFAPMSRSPSLDDWK 776

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVF ARKRALVYILEEDEDEAS
Sbjct: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFGARKRALVYILEEDEDEAS 776

Query: 1021 DAE 1024
            DAE
Sbjct: 1021 DAE 776

BLAST of Spg011683 vs. ExPASy TrEMBL
Match: A0A1S3CJ41 (Anaphase-promoting complex subunit 4 OS=Cucumis melo OX=3656 GN=LOC103501540 PE=4 SV=1)

HSP 1 Score: 1300.4 bits (3364), Expect = 0.0e+00
Identity = 708/1023 (69.21%), Postives = 740/1023 (72.34%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            GRSIKSLCWRPDGKVIAVGLEDGTV LHDVENGKLLRS+KSHAVAVVSLNWV DSQLITD
Sbjct: 61   GRSIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITD 120

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
            KNE LS+YEDRT RIFPPAPT+PRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG
Sbjct: 121  KNEILSTYEDRTRRIFPPAPTIPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFG+FPIGK+NIH+LHIPL  QDASASCHLLNAEIYKVALSKD CRL+VMC
Sbjct: 181  DKDGSICFSIFGVFPIGKINIHELHIPL--QDASASCHLLNAEIYKVALSKDFCRLVVMC 240

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVG GHDPR RQITVQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNIGELTEVIR 
Sbjct: 241  SGELVGHGHDPRKRQITVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRV 300

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFDSLSTLI+NHGLDSS QEEFLS+LGGARTSPPVHQFLVNS
Sbjct: 301  SLSVMSKQWSDAMHTFREKFDSLSTLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNS 360

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKA+SGAGSELQLIVLDHLQPA EIIGFRMGELL +SRWRARFQGVGLDEK
Sbjct: 361  LGEVGAKRVSKAISGAGSELQLIVLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEK 420

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            LM+NA EK G LLVQV+RFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 421  LMHNATEKVGTLLVQVERFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            VIFLKFLYNQDPV KLLEASEND+NIEIDS                              
Sbjct: 481  VIFLKFLYNQDPVNKLLEASENDNNIEIDS------------------------------ 540

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 541  ------------------------------------------------------------ 600

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E+VERVRELALFGGF+DCEFLRR
Sbjct: 601  -------------------------------------ELVERVRELALFGGFADCEFLRR 660

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLG+EFQQMES                                                 
Sbjct: 661  TLGLEFQQMES------------------------------------------------- 720

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 721  ----------------------------------------------------------SF 774

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPF TISRKILCED++SLFPF SSSSCLSS VPLSVSYYE              S
Sbjct: 781  KEAFLMPFITISRKILCEDIISLFPFPSSSSCLSSHVPLSVSYYE-------------DS 774

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SE V AD SC+Q FIDYISF+VPDDSFA+IANCVGI R FIHDQSCSNED+SS EAVL+S
Sbjct: 841  SEVVPADLSCEQKFIDYISFRVPDDSFAEIANCVGIVRRFIHDQSCSNEDHSSSEAVLIS 774

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGS+CVDLSLYKDGQIVLLLNETASTSESSVGSYMMVV+ +DLPFVS+PRSPCLDNW 
Sbjct: 901  IPDGSQCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVKVDDLPFVSVPRSPCLDNWK 774

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            + QLKD VVPLQMENEKVRNI HAV+ PLAVSASRGVACVFAARKRALVYILEE+EDE S
Sbjct: 961  ILQLKDNVVPLQMENEKVRNISHAVISPLAVSASRGVACVFAARKRALVYILEENEDEVS 774

Query: 1021 DAE 1024
            DAE
Sbjct: 1021 DAE 774

BLAST of Spg011683 vs. ExPASy TrEMBL
Match: A0A5A7TZ68 (Anaphase-promoting complex subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G001730 PE=4 SV=1)

HSP 1 Score: 1297.3 bits (3356), Expect = 0.0e+00
Identity = 707/1023 (69.11%), Postives = 738/1023 (72.14%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 65   METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 124

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            G SIKSLCWRPDGKVIAVGLEDGTV LHDVENGKLLRS+KSHAVAVVSLNWV DSQLITD
Sbjct: 125  GESIKSLCWRPDGKVIAVGLEDGTVLLHDVENGKLLRSLKSHAVAVVSLNWVEDSQLITD 184

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
            KNE LS+YEDRT RIFPPAPT+PRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG
Sbjct: 185  KNEILSTYEDRTRRIFPPAPTIPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 244

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            DKDGSICFSIFG+FPIGK+NIH+LHIPL  QDASASCHLLNAEIYKVALSKD CRL+VMC
Sbjct: 245  DKDGSICFSIFGVFPIGKINIHELHIPL--QDASASCHLLNAEIYKVALSKDFCRLVVMC 304

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            SGELVG GHDPR  QITVQGVHGMHSLVLDTSIFRKRK ELHQVAQQASNIGELTEVIR 
Sbjct: 305  SGELVGHGHDPRKIQITVQGVHGMHSLVLDTSIFRKRKSELHQVAQQASNIGELTEVIRV 364

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVMSKQWSDAMHTF+EKFDSLSTLI+NHGLDSS QEEFLS+LGGARTSPPVHQFLVNS
Sbjct: 365  SLSVMSKQWSDAMHTFREKFDSLSTLIVNHGLDSSAQEEFLSILGGARTSPPVHQFLVNS 424

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVGAKRVSKA+SGAGSELQLIVLDHLQPA EIIGFRMGELL +SRWRARFQGVGLDEK
Sbjct: 425  LGEVGAKRVSKAISGAGSELQLIVLDHLQPAAEIIGFRMGELLGISRWRARFQGVGLDEK 484

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            LM+NA EK G LLVQV+RFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV
Sbjct: 485  LMHNATEKVGTLLVQVERFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 544

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            VIFLKFLYNQDPV KLLEASEND+NIEIDS                              
Sbjct: 545  VIFLKFLYNQDPVNKLLEASENDNNIEIDS------------------------------ 604

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 605  ------------------------------------------------------------ 664

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                 E+VERVRELALFGGF+DCEFLRR
Sbjct: 665  -------------------------------------ELVERVRELALFGGFADCEFLRR 724

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TLG+EFQQMES                                                 
Sbjct: 725  TLGLEFQQMES------------------------------------------------- 784

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 785  ----------------------------------------------------------SF 838

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            KEAFLMPF TISRKILCED++SLFPF SSSSCLSS VPLSVSYYE              S
Sbjct: 845  KEAFLMPFITISRKILCEDIISLFPFPSSSSCLSSHVPLSVSYYE-------------DS 838

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
            SE V AD SC+Q FIDYISF+VPDDSFA+IANCVGI R FIHDQSCSNED+SS EAVL+S
Sbjct: 905  SEVVPADLSCEQKFIDYISFRVPDDSFAEIANCVGIVRRFIHDQSCSNEDHSSSEAVLIS 838

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            IPDGS+CVDLSLYKDGQIVLLLNETASTSESSVGSYMMVV+ +DLPFVS+PRSPCLDNW 
Sbjct: 965  IPDGSQCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVKVDDLPFVSVPRSPCLDNWK 838

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDEAS 1020
            + QLKD VVPLQMENEKVRNI HAV+ PLAVSASRGVACVFAARKRALVYILEEDEDE S
Sbjct: 1025 ILQLKDNVVPLQMENEKVRNISHAVISPLAVSASRGVACVFAARKRALVYILEEDEDEVS 838

Query: 1021 DAE 1024
            DAE
Sbjct: 1085 DAE 838

BLAST of Spg011683 vs. TAIR 10
Match: AT4G21530.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 942.2 bits (2434), Expect = 3.5e-274
Identity = 521/1018 (51.18%), Postives = 623/1018 (61.20%), Query Frame = 0

Query: 1    METDEAERVLPFQLQFDKPIASQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 60
            M +DE E ++PFQLQFDKPI  Q+KIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP
Sbjct: 4    MASDEEENIIPFQLQFDKPIPFQIKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISP 63

Query: 61   GRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLITD 120
            G+ + SLCWRPDGK IAVGLEDGT+SLHDVENGKLLR++K H VAVV LNW  D Q  TD
Sbjct: 64   GKPVTSLCWRPDGKAIAVGLEDGTISLHDVENGKLLRNLKPHDVAVVCLNWEEDGQSNTD 123

Query: 121  KNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGFIDDSEDSFTELSNSSQQRFNILCSG 180
            ++ N S YEDRTSR FPPAP  P+MPGLV+GD+ F+DD EDS  ELSN+S ++FNILC+G
Sbjct: 124  ESGNFSVYEDRTSRFFPPAPRPPKMPGLVAGDSSFMDDGEDSLAELSNTSFRKFNILCTG 183

Query: 181  DKDGSICFSIFGIFPIGKVNIHKLHIPLQVQDASASCHLLNAEIYKVALSKDLCRLIVMC 240
            D+DG+ICFSIFGIF IGK+NIH+L +P+   D  ASC L NA IYKVALSKDLCRL+VMC
Sbjct: 184  DRDGNICFSIFGIFQIGKINIHELSLPVPHLDEHASCKLFNASIYKVALSKDLCRLVVMC 243

Query: 241  SGELVGDGHDPRNRQITVQGVHGMHSLVLDTSIFRKRKGELHQVAQQASNIGELTEVIRA 300
            +GEL      PR  +I VQ + G+H L +DTSIF KRK ELHQVAQQASNI +LTEVIRA
Sbjct: 244  TGELKDCDIKPREEKINVQDLPGLHCLAMDTSIFWKRKYELHQVAQQASNIEDLTEVIRA 303

Query: 301  SLSVMSKQWSDAMHTFQEKFDSLSTLIMNHGLDSSPQEEFLSLLGGARTSPPVHQFLVNS 360
            SLSVM+KQW+DAM TF EKF SLSTLI+++GL+SSPQEEFLSLLGGAR SP ++QFLVNS
Sbjct: 304  SLSVMNKQWADAMKTFHEKFHSLSTLIIDNGLESSPQEEFLSLLGGARISPALNQFLVNS 363

Query: 361  LGEVGAKRVSKAVSGAGSELQLIVLDHLQPAVEIIGFRMGELLALSRWRARFQGVGLDEK 420
            LGEVG KRV K+V G G ELQ +VLDHLQPA EIIGFR+GEL  LSRWRAR+QG+GLDE 
Sbjct: 364  LGEVGVKRVLKSVCGTGKELQQVVLDHLQPAAEIIGFRIGELRGLSRWRARYQGIGLDEM 423

Query: 421  LMNNAMEKAGMLLVQVDRFMRVLSTVLQQFSNFFNWLIRCIKLLMSEPSDQLLPYNSELV 480
            L+N A E  G+LLVQV RFM VLS+V+QQFSNFFNWL+R IK LM EP+DQLL YNSEL+
Sbjct: 424  LLNEATENTGLLLVQVQRFMMVLSSVVQQFSNFFNWLVRSIKYLMQEPNDQLLSYNSELL 483

Query: 481  VIFLKFLYNQDPVKKLLEASENDDNIEIDSETMDVLALLALLSLMTIAFTLMGDVCFWTL 540
            V+FLKFLY+QDPVK LLE SE  D+IEID +T                            
Sbjct: 484  VVFLKFLYDQDPVKDLLELSEAGDDIEIDLKT---------------------------- 543

Query: 541  VPSKVSLIYLSFNAWLVPPRGGSIFSLAWKECGGGLGSFALELSVSLCFVELLCFRSSAS 600
                                                                        
Sbjct: 544  ------------------------------------------------------------ 603

Query: 601  TLVASRVVKRQLRSSSIRPFHEKGRFLWQVRVCAILWEVVERVRELALFGGFSDCEFLRR 660
                                                   + RV+EL  FGGFS+C+FL+R
Sbjct: 604  ---------------------------------------IGRVKELLQFGGFSECDFLQR 663

Query: 661  TLGMEFQQMESRRNMNDREAKEYARLLLMENLQPNLKDSRRGTVLSSLNDYGDSCKRSMR 720
            TL  EFQ MES                                                 
Sbjct: 664  TLAKEFQHMES------------------------------------------------- 723

Query: 721  CGGSFRRGPQTEFNSWLGLVSLIYPIRLGDDGDENQDHIFLRCPFAVRGWEQIVKAFGSF 780
                                                                      SF
Sbjct: 724  ----------------------------------------------------------SF 771

Query: 781  KEAFLMPFSTISRKILCEDLLSLFPFQSSSSCLSSSVPLSVSYYEVFILLLSILYVAFGS 840
            K AF MPF+TISRKI C  LL L P Q S++   +++P+S+S+Y+               
Sbjct: 784  KMAFQMPFTTISRKISCMKLLPLCPLQLSTTQTPTTIPMSLSFYK--------------- 771

Query: 841  SEAVSADQSCQQNFIDYISFKVPDDSFADIANCVGIARGFIHDQSCSNEDYSSFEAVLVS 900
               +S D  CQ  + DYISF+VPD++F +I+NC+GIA+G+  + +     Y+S EAVL+S
Sbjct: 844  -NELSDDTPCQSGYTDYISFQVPDETFPEISNCIGIAKGYKQNSNNEKNGYTSLEAVLLS 771

Query: 901  IPDGSRCVDLSLYKDGQIVLLLNETASTSESSVGSYMMVVQANDLPFVSMPRSPCLDNWN 960
            +P+G  CVDLSLYKD ++VLLLN+T + SE S  + MMVVQ  DL F+S+  S  L+ W 
Sbjct: 904  VPNGYTCVDLSLYKDKELVLLLNKTNTDSEGSGEACMMVVQTGDLAFISISGSSSLNQWE 771

Query: 961  VHQLKDYVVPLQMENEKVRNIPHAVVPPLAVSASRGVACVFAARKRALVYILEEDEDE 1019
            +  LK  +V L+MENEKVR +PH+V+ PLAVSASRGVACVFA R+RALVYILEEDEDE
Sbjct: 964  LEDLKGSIVNLEMENEKVRKVPHSVIAPLAVSASRGVACVFAERRRALVYILEEDEDE 771

BLAST of Spg011683 vs. TAIR 10
Match: AT5G25150.1 (TBP-associated factor 5 )

HSP 1 Score: 48.9 bits (115), Expect = 2.8e-05
Identity = 27/107 (25.23%), Postives = 48/107 (44.86%), Query Frame = 0

Query: 22  SQVKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGRSIK----------SLCWRP 81
           S V   +W+P  + +A  + D  +        RLW +  G  ++          SL   P
Sbjct: 503 SDVDCVQWHPNCNYIATGSSDKTV--------RLWDVQTGECVRIFIGHRSMVLSLAMSP 562

Query: 82  DGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNWVVDSQLI 119
           DG+ +A G EDGT+ + D+   + +  +  H   V SL++  +  L+
Sbjct: 563 DGRYMASGDEDGTIMMWDLSTARCITPLMGHNSCVWSLSYSGEGSLL 601

BLAST of Spg011683 vs. TAIR 10
Match: AT4G29830.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 3.1e-04
Identity = 35/122 (28.69%), Postives = 59/122 (48.36%), Query Frame = 0

Query: 59  SPGRSIKSLCWRPDGKVIAVGLEDGTVSLHDVENGKLLRSVKSHAVAVVSLNW-VVDSQL 118
           S  + + S+ W P+GK +A G  DGT+ + DV+  KLL  ++ H + V SL +  VD ++
Sbjct: 156 SSKKFVLSVAWSPNGKRLACGSMDGTICVFDVDRSKLLHQLEGHNMPVRSLVFSPVDPRV 215

Query: 119 I----TDKNENLSSYEDRTSRIFPPAPTVPRMPGLVSGDTGF---IDDSEDSFTELSNSS 173
           +     D + N+   E +T            + G +SG T +   +D S D     + SS
Sbjct: 216 LFSGSDDGHVNMHDAEGKT------------LLGSMSGHTSWVLSVDASPDGGAIATGSS 265