Spg011675 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg011675
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNA polymerase II transcription factor B subunit 2
Locationscaffold1: 3149091 .. 3156873 (+)
RNA-Seq ExpressionSpg011675
SyntenySpg011675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTCGTTTTTGTCTTCGGTCTTCCGAATGATCGTCGGACCAAGCAGAAGGTCATCGCCAAATCATGAAGGTTGAGCTGCTGTTAATCAATTGATTGAATTCGAAGGATACCAGTCTGGAGTCTGGACCATTGGCTATAAATACTCTTTGCAGTCTCACTGGCGAACTGGAAGCTGCAACCGTAGGTAATTTATCCAACACTTCTGGGATCCTCCTTTGCTTGCTCAAAGTTTTGCAGAACCCTAAAGGACTAGCTGGAAAGAGAACTATAACGAAGGAAAAGAAGGAAAGCTCAATTCAGGATGCCTCAAGTGAAGATCATAGCGAAGAATTTTATGGACATGGTGGCCTCCTTGCCCGCCATGAAGCTCGACAAGCTCTATGAGAATGCATTCATCTGCGAAGCCATTCTCAGGTCCTTGCTTGCGACCTCAAAAATGCTTTAATTGTTTCGGGTCGTATTGTTACTGCAAAGCAAAAAATACCCAAAACGACTAAAACCTTGGGTTTGTATGTAATTGTGTCAAGTGGTGCTCTGGACATCTGAGCATGATTTTCATATCCTGGCGTCCTTTTGATAATATGATGTCATTGGAGCTGATTTTTCTTTTATTTATTCGATCTAAAATGTTCTAGATTTGATATTGATTCTGAATTTCCGTACTTCTTAATTAGTTGTTTGGTCATTTTCCCTTCTCGTTCTTTGAGTTAGAGTACTGTGGCTAAAGTTCTTGGTATTTGTAAATGGGTATAACTTAAAAGTTGCTAGTTAATCCTAGTTGCGGGGTTTTCATTTATAAACAAGAATGCCAATCTTTTTTTTTTTTTTTCCTTCTCTTTTTGGTAATCAGGTCACTTCCACCACTGGCCAAGAAGTTTGTTTTACAAATGTTATACATTGATGCTCCAGTAACAGCCAAGTCCATAGAGGAGTGGGTGCTTCCAGATGGAGTCTCAAAGTATAAGGTTGCCATTGATCGATTGATTCAATTGAGAGTATTTATTGAGACTGCGGATAGGTGTTCTTTCTCTTCTCTCATTTTCTTAAGAATTTGATACTCTTTCACAATGCTTTTTGAATGCATCCTTAGTTAAATAGAGCCAAGAAAGCTGACACTTAATGTTGCCAAGTCTGTGGAGTTTTTATCTTCATTTTTTTCTCCGATGATTCTTTTCCATCAGTAGATAGATTGCCCACTTGTTCATATCGTGATATCATCGGAATTGAGAGGTTCATGCAAGGAGAACAATTTGTAATTGCTTTTGCGTTGTTTCTGTTTTGATGTCATTGAAATTGTTTATAATTCTACCAGTAGTTTTGCATGGTTTATAGTCCTCAACCCACTCCTGTATTGTAATATTTTATATGGATCCCTATTTTATTGTTAGGAAAAGAGTCTTATCAAAAAAATTTATTGTTAGGAAAAGAGAAACAACCTACAGGCTAAATCCAACGTTCCAAGCAAACCTCCAAAAGCTTTTAATACATGGGTAAGTTTTCTATCATTTGACTCAATAAATGCATCTTTAAACCAGATATGCAAGGAGGGATACATGATTCTTTTTCATTTTCTTTCTACTCAGCACAAACATTCTGATGCATGAATCTTTCTTAATATATGCACATTTTCTTTCTACTTGCTACCTTTTTGCATTCATCAAGATTCAGCTTGCTACCTTTTTGCATTCATCAAGATTCAGCTTGCTACCTTTTTGCATTCATCAAGATTCAGGTTCAAGTAGATGATTACTCATCTTCTATAAAGAGAAGCCGATCCTGGAGTTTAGACTCCTTGATTTTAACATTTAAAAGTGTTTTACTCTTGGTTTAGAGAATGACCATGAATTTGGGATCTTGCAGTGAAGTTCTAGCCAGAGAACCAATGCCTTCTAATATAACCGTGAGGCTTCCAAGTTTGGAAGATCTTGAGGCTTATGCTCTGGATCAGTGGGAGGTCGGACAGTTATCAAAATGATTTTCATCATCTGTTTTTTTTTGTTGCATCCTTTCTAGTATATTCTTCGGCTATTATTTATTCCATCCCCTGGAATTCAATCAAAGGCAACCAATCTTCTCAGTTTCTTACTGTCCAAGTTTTAATCGTGGATATTCTTCTGACGTGTTATTTTGTTAGTTCAGTGCTTCTTGCTGCAATTGATAAACTCAGGCCAAGCAGAGAAGCCGTCAAATATTAGTTCTTCTGTGATGAAAGTTTTCCAGAAAGGGCTTTTAAGTCAGAGGTTAAAATCTTATTCATTGTAAACTAGCGTACATATAGCGGTCACTTCTTATATCTGATAAGATTTCATGTATGGTATCCAGGGATAAAGAAACTCCACGATTAACTGAGAGTGGTTTCCAGTTCTTGGTATGAATCACATGAACCATAGTCCTCTTGAAATCCTCATTTATCTGTCTCACTAGGGATTCTTGTGATATATGTAAACTTCAGTTGATGGAAACAAACGCACAACTTTGGTATATCATCAGAGAATATATATCTAACTCTGAGGTTTGTGTTTTCCTTTTTTATTGTTATATCCTATTGCCTTCCTCTGATGAAAAACTATATATATTCTGTTATTAATTTGGAACACAATTTCTATTTGAATCCAAATGATGGATTGTTCTTACTCAAAACGTAAAGCAATTATTGTTATTTATTTTCTTCTAATATCTCATATTTCTCTCTAGTAAATGGTTTTGTTTTATTTTTATTTTTATTTTTTTCTTTTTTTTGACAAACTCCTTTGTAAATGCTTATCAATATGGTGCAGGAGCGAGATGTGGATCCTGCAGATTTGATTTCTTTTCTGCTAGAGCTTAGTTTTCATGTGACAGGAGAGGTATCAATTGTTTGATTATCTTTTAATTCCTTATTTTCATCTTTATCTTTTATTGTTGTATTCTTTTATCTCATTACAGAACTCTCTTTTAAGTTTCGTTGCTTCATATCATTTTAATACTGAACTACTTTGGGTTGGTTCAGGATTATAAATTCTAACGCATATTATTTATATTTAGTTTGTAATTTGAAAGCCCACAAAACACTGTGCACTAATCCTAATTAATCTTAGCTTGTTTATGGTTAGGTTGAATTTGATTGGAATTTTATCCCTATTTAATTTTTTTTATTTATATAATGATAGTCACTCTTTTATGACTTGTTTGTATGTTTTAGGAAATAAACAAATTTCTTTAGGGTTCTTTTTCCCTCCATCATAAGAGTGAATAATTGTATTAATGATGGAGAAGAAGTACAAGTGGAGGTAAGGTATCAACTCAATTAAGAGCCACTGGGATTACATTAAATTCCTTCCATTGACATTGACAAATAAGAACAATAATTACAAGGTAGAATATAGGAGCTTCATATAGATGCGTTGAGTTTGATCATCTTACACAACCATCTTTCCCTCTAAACTTATCCTGAAAATGTCTATAGTTCCTTTCATCAACGCTGACAGCGGTGTGGTCTTAGCAGCACAAAATGCTCTTGTTTTGAGTTTCCATATTTGCTGTGCACGTGATACACAGGGAGACAGGGATCATATGGGTTTCTCTTTTGAGTAAGCTTGGCTTGTATTTATCCTCCGCCCCCCCATAGCGAGATGCAAAATGAAAAATATAATTTCTTTGGGTTGTCATGCCACCACACCAAGGAACCTTAAATAACCACTTTGGGATTAAAAAAAAAAACAAGATAATCATTACAAATCTAGAATTCAGAATCATTTAGAGGTCTATTGAAATCTATCTTTTAGTTCTCCTTCTCGAATTCCAGCAGTCAGCTAACTCTTCATTTTTAGAGCTACACAAGAACTCTAGAGGAAGCTAGCTTCAAAGGAATATTCGAGGTCTATGTTCTTTCATAGACGCTGATTTTGGTTCCGTCACCAATTTTGGAGAAACTCCTTATTTTCTCTTTGCACCTCACCACAAACTTCCGAGGGATGCTGCTTCTCATTTTCCTATGGTGAACAAATAGATGTAAATCAATTGTCCCTATCAATGCCATTTAGACACAAGTGCTAAATTGCTCTCCTTAAAATTGCCTACTCCAAATCTACGCTCTTTTGGTGGTTAGACACCCTCTTCCAATCAGCTAGACGACTGGCATTGTCAATGCCCTCTCCCCATGGGAGATTTCTCAACATACTCTCCAAAAGCTTAGCCACTTGGATTGTCAATAGACACATCAAATAAATAGATAGGAACACTCAACACTGTAAATTTCACTAAAGTGAGTCTTCCACCCTTAGACAAAATGGCACTCGTACATTTTTTCTTTATGAAACCTCTGGAGCTGATTTTGCTTCCACTCCCGTAGTTCCAAGTGGGTACTAACCTGTTTACAAAATTCTTCCCTTTGAAACTCAAGTAAGAAAAAAAAAAATAAAACAAACAAACAAATTCCAATAATCCGTATAATTAACTGTTAGAAAACTAATCCATATACTATTTACTGCCCAATAGCCAATGTTTTTTCTTTGTTTTGTAGGCTTACGACATTGATACATTGACAGAAGAACAGAGATATGCTATCAAGGACCTTGCTGATCTGGGACTAGTTAAGCTTCAGCAGGTTTTTGGCTAGTCTCTGCCACTTTTTAAATTGAATATGACCTTTGCTCAAGTTCTTACTTTAATTTAACTTCACCTTTTTTCTTTTTCTTTTTCTTTTGTTGTGTGCTTTTCACTGTTAATTTGTCCAGGGTAGAAAAGACAGTTGGTTCATACCTACTAAATTAGCCACAAATCTTTCAATGAGTTTGGCAGATTCTTCTTCAAGGAAACAGGTGAGTATTTCTAAATTATATTTAATTTTGATATATTTCTCTGAGGTTCATTTTCTTTATGGCTACTAAATTTTTTAGTAGTTTTTGTCTTTTGCATGATAGCTTTTCCATAATTATTTTTTAGCTATACATATAGAGAGTATCCTCTAGTATATATCTTATCCTCGTTACTTCTTCTGTAAGTTTTATGGGGTCTTTGGGGTGAGAGAAACAATAGAGTTTTTCGTGGTATTGAGAGAGATCCTTCTGATGTATGGGCCCTCACTAGATTCTATGTTTCTCTTTGGGCCTCTGTGTCCAAGGCTTTTTGTAATTATATGATAGGTTCTATCATTTTGAGTTGGTGGCCATTCCTTTAAGGGAGGCATTTTTTTGTGGGCTGGGTTTTTTGTATGCCCGTGTATTCTTTCATTTTTTCTCAATGAAAGTTGGTTTTTTCATCAAAAAAAAAAAAAAAAAAAACTCAATCCATTGTCCTCTTTTTGTTGTTTATGGGTTTGGAGGCTCTATGGAAATTTTTGTTCTTACAAATTTTCTTCCTGTTGTATACCCAGGGATTTGTTGTGGTGGAGACGAATTTCAGGATGTATGCTTATTCTTCTTCCAAACTACATTGTGAAATATTACGTCTTTTTTCAAGGTAGATGTCATCAATTAATGTTGTATTACATTATATAACCAATGTTGAACAAGTTAGTTTTAACCACTATGAGGCCTAATGGATCAATAAGAGCCAACGTAATTAGCCAAGGACTTGGAAGGAAAATTTATGGTGGCCACGTACACAATTTGGAGGTAGGAGGATTCAAACTCATGACCTCTTGGTCATAAGCACACACTTTATACTAGTTATACTAGTTGAGCTATGCTCTTGTTGGCCAAACGCTAAAGGATATTAAACAAAAGATTACAAAATTGATTGCCTACTGGACAGTGGATACATTTAATATTGACGTCAAAAGATTACTTTCTCTTCTTCTTGAATGCTAAATATATTCGAATTTCCCCACCCTGATTGACCCCACTTGAAACTTCACATACGTTAGGAGGAAGAAAGAGGGGCAAGCTGAGGGGTAACCTAGTCATTAGCGTTGCTTCTTGAGAGTGTAAGCAACTAGTGATACATTCTGTAAGGTCAGGGCCAATTAATCATACTATTACATAATTCGTTAATGATAATTCAATAATTATGGTGTTATACTTTTGTAAAGTATTCTTCTAAAAAAGAAATACATGTGTAAATTAGAAATGCTGGCACTTTTGTAACTATAGATAATTTCTCTTTTACTTTTATATCAATTTTTTACAACAGATTCATCTGGTTATAAAATGGTTTGTTTTTTAATTTTTTTTTACCAGAAGCTTTTGTAATAGTTGATTAAATAGGGATATGATTCAAGTTCAACATTCTACATTGTCCCTCTGCAGGATTGAGTATCAACTTCCAAATCTTATAGTTGCAGCAATAACGAAAGAAAGCTTGTATAATGCTTTTAAGAACGGAATTACAGCAGAGCAGGCAAGTTTTGCTGCTTGTTCACGTGGCAGTTACTCGTAGTCTTAGTCTTATGAAGTAAGGATATCATTATCTTATGTTCAAATTGCTAATGGAATGCAATAGACTAGTTATTCTTTGTGCTTCATTAGTTTACGATATCTTGCGCTTCATTCTTGCTAGTGGCGGAATACTTGGAGAATTCTCCCTAGTTCAAACCTTTATTCTGTACAAGTAGCATGCAAAAATATTGGGGCTTGGCACAAGGGGATCATTTCTATTAAGCAGGGATCAAGTGCCAAACCCATTGTTATCGCCCAATCACCAAGGGACTTCAACTAGCATTCGCTCCTAATCCACATGAAACAATATTTCTATTTTTCTTGCGTGCATCAATCTAAATACTCTATAAACTTAATGCAAACTCGAATAATTCTTTTGAGCTCCAAATATTAAAATGAGCAACACATAGGTATTCAATTACATTAAATACATTACTATTAATTTTTCTATGCTGGTTTTTTCATTTCAAATTGCTTGATATCTGACTGCTGTTTTGCAGATAGTTACTTTTCTACAGCAAAATGCACATCCTCGTGTTGCGGAGAGAATACCATCAGTCCCTGAAAATGTCACGGATCAGGTTAGGATTGCTTTACTTAGTTCTTGATGTTTGGCAAGCCAATAGCTTCCATCTGCAAATCACCCGTGCTTGCCATGAAAAACCAAAACTTTCTATACATGTTCTCATCTGACAATTTTATCAAGTCTTTGTCCTGTTCCTGATTTTTGTCCATTGCTCTCTATTCCATGACAGATTAGGTTATGGGAATCAGATCTTAATAGGGTCGATATTACTCCTGCACATTTTTACGACGAATTCCCTTCTAGGGTATGGATTTCAAATGTTGCTCCTTTAAACTTCGAAGATTACTTGTACATTCACTGAAATATCACCTGACAAGGACCTTCTTTGGCATGCCAGGAAGTTTTCGAGGCTGCTTGCGACTACGCACGAGAATGGAATGGGTTGCTATGGGAGGACTCGAAAAACATGCGGTTGGTCGTGAAGGCAGACATACACACACATATGCGGGAACATCTTCGCCGTCAAAAATAGACCGTGAAGAAATGATACAATACTGCTGCTAGATCTTGTACATAACCATGGCCCCTGGTACATCAGTTTTTGTTGCTTTGGCATGGACAAAAGATTTAGTAAGTTAATGAAAATTGTGAAGAGCGCAGTGAGAGTTTTGTGGGAAAAAGAATGCTCTGTGCCAATGGCAATGAGTTTATTACTGTGTACGTTTATATTTTTCAAAGTTAGTTTCTCTTCTTCATAGTTCATACGACACGGAAGTTTTGTTAGATTGATTTCCCTTCGTTGATCAACGTCTGGGCATAGGTTCATTCTACCATTTGTTTACCACCGGGACTTTATGTTTAAAAAATTTGGTAATCTATTCTATTCTATTTGGTTCAGCC

mRNA sequence

ATGCCTCAAGTGAAGATCATAGCGAAGAATTTTATGGACATGGTGGCCTCCTTGCCCGCCATGAAGCTCGACAAGCTCTATGAGAATGCATTCATCTGCGAAGCCATTCTCAGGTCACTTCCACCACTGGCCAAGAAGTTTGTTTTACAAATGTTATACATTGATGCTCCAGTAACAGCCAAGTCCATAGAGGAGTGGGTGCTTCCAGATGGAGTCTCAAAGTATAAGGTTGCCATTGATCGATTGATTCAATTGAGAGTATTTATTGAGACTGCGGATAGGAAAAGAGAAACAACCTACAGGCTAAATCCAACGTTCCAAGCAAACCTCCAAAAGCTTTTAATACATGGCTTGCTACCTTTTTGCATTCATCAAGATTCAGCTTGCTACCTTTTTGCATTCATCAAGATTCAGGTTCAAGTAGATGATTACTCATCTTCTATAAAGAGAAGCCGATCCTGGATTCAGTGCTTCTTGCTGCAATTGATAAACTCAGGCCAAGCAGAGAAGCCGTCAAATATTAGTTCTTCTGTGATGAAAGTTTTCCAGAAAGGGCTTTTAAGTCAGAGGGATAAAGAAACTCCACGATTAACTGAGAGTGGTTTCCAGTTCTTGGAGCGAGATGTGGATCCTGCAGATTTGATTTCTTTTCTGCTAGAGCTTAGTTTTCATGTGACAGGAGAGGCTTACGACATTGATACATTGACAGAAGAACAGAGATATGCTATCAAGGACCTTGCTGATCTGGGACTAGTTAAGCTTCAGCAGGGTAGAAAAGACAGTTGGTTCATACCTACTAAATTAGCCACAAATCTTTCAATGAGTTTGGCAGATTCTTCTTCAAGGAAACAGATAGTTACTTTTCTACAGCAAAATGCACATCCTCGTGTTGCGGAGAGAATACCATCAGTCCCTGAAAATGTCACGGATCAGATTAGGTTATGGGAATCAGATCTTAATAGGGTCGATATTACTCCTGCACATTTTTACGACGAATTCCCTTCTAGGGAAGTTTTCGAGGCTGCTTGCGACTACGCACGAGAATGGAATGGGTTGCTATGGGAGGACTCGAAAAACATGCGGTTGGTCGTGAAGGCAGACATACACACACATATGCGGGAACATCTTCGCCGTCAAAAATAG

Coding sequence (CDS)

ATGCCTCAAGTGAAGATCATAGCGAAGAATTTTATGGACATGGTGGCCTCCTTGCCCGCCATGAAGCTCGACAAGCTCTATGAGAATGCATTCATCTGCGAAGCCATTCTCAGGTCACTTCCACCACTGGCCAAGAAGTTTGTTTTACAAATGTTATACATTGATGCTCCAGTAACAGCCAAGTCCATAGAGGAGTGGGTGCTTCCAGATGGAGTCTCAAAGTATAAGGTTGCCATTGATCGATTGATTCAATTGAGAGTATTTATTGAGACTGCGGATAGGAAAAGAGAAACAACCTACAGGCTAAATCCAACGTTCCAAGCAAACCTCCAAAAGCTTTTAATACATGGCTTGCTACCTTTTTGCATTCATCAAGATTCAGCTTGCTACCTTTTTGCATTCATCAAGATTCAGGTTCAAGTAGATGATTACTCATCTTCTATAAAGAGAAGCCGATCCTGGATTCAGTGCTTCTTGCTGCAATTGATAAACTCAGGCCAAGCAGAGAAGCCGTCAAATATTAGTTCTTCTGTGATGAAAGTTTTCCAGAAAGGGCTTTTAAGTCAGAGGGATAAAGAAACTCCACGATTAACTGAGAGTGGTTTCCAGTTCTTGGAGCGAGATGTGGATCCTGCAGATTTGATTTCTTTTCTGCTAGAGCTTAGTTTTCATGTGACAGGAGAGGCTTACGACATTGATACATTGACAGAAGAACAGAGATATGCTATCAAGGACCTTGCTGATCTGGGACTAGTTAAGCTTCAGCAGGGTAGAAAAGACAGTTGGTTCATACCTACTAAATTAGCCACAAATCTTTCAATGAGTTTGGCAGATTCTTCTTCAAGGAAACAGATAGTTACTTTTCTACAGCAAAATGCACATCCTCGTGTTGCGGAGAGAATACCATCAGTCCCTGAAAATGTCACGGATCAGATTAGGTTATGGGAATCAGATCTTAATAGGGTCGATATTACTCCTGCACATTTTTACGACGAATTCCCTTCTAGGGAAGTTTTCGAGGCTGCTTGCGACTACGCACGAGAATGGAATGGGTTGCTATGGGAGGACTCGAAAAACATGCGGTTGGTCGTGAAGGCAGACATACACACACATATGCGGGAACATCTTCGCCGTCAAAAATAG

Protein sequence

MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTAKSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLPFCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMKVFQKGLLSQRDKETPRLTESGFQFLERDVDPADLISFLLELSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSSSRKQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEAACDYAREWNGLLWEDSKNMRLVVKADIHTHMREHLRRQK
Homology
BLAST of Spg011675 vs. NCBI nr
Match: XP_023543805.1 (RNA polymerase II transcription factor B subunit 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615.1 bits (1585), Expect = 3.9e-172
Identity = 337/459 (73.42%), Postives = 349/459 (76.03%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDQLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTY+LNPTFQANLQKLLIHG + 
Sbjct: 61  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYKLNPTFQANLQKLLIHGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++ +       +    +CFLLQLINSGQA+KPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPNLEELEAYALDQWECFLLQLINSGQADKPSNISSSVMK 180

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKETPRLTESGFQFL                    ERDVDPADLISFLLE
Sbjct: 181 VFQKGLLSQRDKETPRLTESGFQFLLMETNAQLWYIIREYISNAEERDVDPADLISFLLE 240

Query: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300
           LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS
Sbjct: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300

Query: 301 SRK--------------------------------------------------------- 360
           SRK                                                         
Sbjct: 301 SRKQGFVVVETNFRMYAYSSSKLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGIT 360

Query: 361 --QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 381
             QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA
Sbjct: 361 AQQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 420

BLAST of Spg011675 vs. NCBI nr
Match: KAG6604444.1 (General transcription and DNA repair factor IIH subunit TFB2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615.1 bits (1585), Expect = 3.9e-172
Identity = 337/459 (73.42%), Postives = 349/459 (76.03%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA
Sbjct: 32  MPQVKIIAKNFMDMVASLPAMKLDQLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 91

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTY+LNPTFQANLQKLLIHG + 
Sbjct: 92  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYKLNPTFQANLQKLLIHGEVL 151

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++ +       +    +CFLLQLINSGQA+KPSNISSSVMK
Sbjct: 152 AREPMPS--------NITVRLPNLEELEAYALDQWECFLLQLINSGQADKPSNISSSVMK 211

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKETPRLTESGFQFL                    ERDVDPADLISFLLE
Sbjct: 212 VFQKGLLSQRDKETPRLTESGFQFLLMETNAQLWYIIREYISNAEERDVDPADLISFLLE 271

Query: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300
           LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS
Sbjct: 272 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 331

Query: 301 SRK--------------------------------------------------------- 360
           SRK                                                         
Sbjct: 332 SRKQGFVVVETNFRMYAYSSSKLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGIT 391

Query: 361 --QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 381
             QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA
Sbjct: 392 AQQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 451

BLAST of Spg011675 vs. NCBI nr
Match: XP_022977542.1 (RNA polymerase II transcription factor B subunit 2 [Cucurbita maxima])

HSP 1 Score: 612.5 bits (1578), Expect = 2.5e-171
Identity = 336/459 (73.20%), Postives = 348/459 (75.82%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDQLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTY+LNPTFQANLQKLLIHG + 
Sbjct: 61  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYKLNPTFQANLQKLLIHGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++ +       +    +CFLLQLINSGQA+KPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPNLEELEAYALDQWECFLLQLINSGQADKPSNISSSVMK 180

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKETPRLTESGFQFL                    ER VDPADLISFLLE
Sbjct: 181 VFQKGLLSQRDKETPRLTESGFQFLLMETNAQLWYIIREYISNAEERGVDPADLISFLLE 240

Query: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300
           LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS
Sbjct: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300

Query: 301 SRK--------------------------------------------------------- 360
           SRK                                                         
Sbjct: 301 SRKQGFVVVETNFRMYAYSSSKLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGIT 360

Query: 361 --QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 381
             QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA
Sbjct: 361 AQQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 420

BLAST of Spg011675 vs. NCBI nr
Match: XP_022925808.1 (RNA polymerase II transcription factor B subunit 2 [Cucurbita moschata])

HSP 1 Score: 609.4 bits (1570), Expect = 2.1e-170
Identity = 335/459 (72.98%), Postives = 347/459 (75.60%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LY NAFICEAILRSLPPLAKKFVLQMLYIDAPVTA
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDQLYGNAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTY+LNPTFQANLQKLLI G + 
Sbjct: 61  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYKLNPTFQANLQKLLIQGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++ +       +    +CFLLQLINSGQA+KPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPNLEELEAYALDQWECFLLQLINSGQADKPSNISSSVMK 180

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKETPRLTESGFQFL                    ERDVDPADLISFLLE
Sbjct: 181 VFQKGLLSQRDKETPRLTESGFQFLLMETNAQLWYIIREYISNAEERDVDPADLISFLLE 240

Query: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300
           LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS
Sbjct: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300

Query: 301 SRK--------------------------------------------------------- 360
           SRK                                                         
Sbjct: 301 SRKQGFVVVETNFRMYAYSSSKLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGIT 360

Query: 361 --QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 381
             QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA
Sbjct: 361 AQQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 420

BLAST of Spg011675 vs. NCBI nr
Match: XP_004134713.1 (general transcription and DNA repair factor IIH subunit TFB2 isoform X1 [Cucumis sativus] >KGN49219.1 hypothetical protein Csa_003950 [Cucumis sativus])

HSP 1 Score: 607.4 bits (1565), Expect = 8.2e-170
Identity = 333/459 (72.55%), Postives = 346/459 (75.38%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LYENAFICEAILRSLPPLAKKFVLQMLYID PV+A
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDQLYENAFICEAILRSLPPLAKKFVLQMLYIDGPVSA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHG + 
Sbjct: 61  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++         +    +CFLLQLINSGQAEKPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPSLEDLEAYALDQWECFLLQLINSGQAEKPSNISSSVMK 180

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKE PRLTESGFQFL                    ER VDPADLISFLLE
Sbjct: 181 VFQKGLLSQRDKEAPRLTESGFQFLLMETNAQLWYIIREYISNAEERGVDPADLISFLLE 240

Query: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300
           LSFHVTGEAYDIDTL++EQRYAIKDLADLGLVKLQQGRK+SWFIPTKLATNLSMSLADSS
Sbjct: 241 LSFHVTGEAYDIDTLSDEQRYAIKDLADLGLVKLQQGRKESWFIPTKLATNLSMSLADSS 300

Query: 301 SRK--------------------------------------------------------- 360
           SRK                                                         
Sbjct: 301 SRKLGFVVVETNFRMYAYSTSKLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGIT 360

Query: 361 --QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 381
             QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA
Sbjct: 361 AEQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 420

BLAST of Spg011675 vs. ExPASy Swiss-Prot
Match: Q680U9 (General transcription and DNA repair factor IIH subunit TFB2 OS=Arabidopsis thaliana OX=3702 GN=TFB2 PE=2 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 2.6e-142
Identity = 277/460 (60.22%), Postives = 323/460 (70.22%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPA+KLDKLY N FICEAILRSLPPLAKK+VLQMLYID PV A
Sbjct: 1   MPQVKIIAKNFMDMVASLPAIKLDKLYNNVFICEAILRSLPPLAKKYVLQMLYIDVPVPA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIH-GLL 120
             +EEWVL DG SK++VAIDRLIQLR+F E +DRKR T+Y LNPTFQ NLQK +I  G+L
Sbjct: 61  TMMEEWVLADGTSKHRVAIDRLIQLRIFSEISDRKRGTSYSLNPTFQNNLQKHIISGGVL 120

Query: 121 P-FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSV 180
           P   ++ D+A  L +  +++             + W +CFLLQLINSGQ EK + ISSS+
Sbjct: 121 PREPMNSDNAIKLPSLQELETYA---------LKQW-ECFLLQLINSGQGEKLTGISSSM 180

Query: 181 MKVFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFL 240
           MK+FQ+GLLSQRDK+ PRLTESGFQFL                    ERDVDPADLISFL
Sbjct: 181 MKIFQRGLLSQRDKDGPRLTESGFQFLLMDTNAQLWYIIREYILNAEERDVDPADLISFL 240

Query: 241 LELSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLAD 300
           LELSFHVTG+AY+++TLTE Q   +KDLADLGLVKLQQGRKDSWFIPTKLATNLS+SLAD
Sbjct: 241 LELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQQGRKDSWFIPTKLATNLSVSLAD 300

Query: 301 SSSRK------------------------------------------------------- 360
           SS+RK                                                       
Sbjct: 301 SSARKEGFVVMETNFRMYAYSTSKLQCEILRLFARIEYQLPNLIACAITKESLYNAFDNG 360

Query: 361 ----QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVF 380
               QI+TFLQQN+HPR A+R+PS+PENVTDQIRLWE+DL R+++T AHFYDEFPS++VF
Sbjct: 361 ITSDQIITFLQQNSHPRCADRVPSIPENVTDQIRLWETDLQRIEMTQAHFYDEFPSKDVF 420

BLAST of Spg011675 vs. ExPASy Swiss-Prot
Match: Q92759 (General transcription factor IIH subunit 4 OS=Homo sapiens OX=9606 GN=GTF2H4 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 9.5e-28
Identity = 113/460 (24.57%), Postives = 193/460 (41.96%), Query Frame = 0

Query: 3   QVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTAKS 62
           +V +  +N  + +  L    LD+LY +   C A+ R LP LAK +V++ML+++ P+   +
Sbjct: 11  RVHLQCRNLQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAA 70

Query: 63  IEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLPFC 122
           +  WV  +     + +   L  LR++             LNP F+ NL+  L+ G   + 
Sbjct: 71  VALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQGLILNPIFRQNLRIALLGGGKAW- 130

Query: 123 IHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMKVF 182
              D    L           D  S  K +    +  L  ++ S  A    +++  +    
Sbjct: 131 --SDDTSQLGP----DKHARDVPSLDKYAEERWEVVLHFMVGSPSAAVSQDLAQLLS--- 190

Query: 183 QKGLL-SQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLEL 242
           Q GL+ S    E P +T +GFQFL                     R +D  +++SFL +L
Sbjct: 191 QAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQL 250

Query: 243 SFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLS-------- 302
           SF   G+ Y ++ +++     ++ L + GLV  Q+ RK   + PT+LA NLS        
Sbjct: 251 SFSTLGKDYSVEGMSDSLLNFLQHLREFGLV-FQRKRKSRRYYPTRLAINLSSGVSGAGG 310

Query: 303 -----------------------------------------------------MSLADSS 362
                                                                 ++A   
Sbjct: 311 TVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGI 370

Query: 363 SRKQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFE 381
           + +QI+ FL+  AHP + ++ P +P  +TDQIRLWE + +R+  T    Y++F S+  FE
Sbjct: 371 TAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFE 430

BLAST of Spg011675 vs. ExPASy Swiss-Prot
Match: P60027 (General transcription factor IIH subunit 4 OS=Pan troglodytes OX=9598 GN=GTF2H4 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 9.5e-28
Identity = 113/460 (24.57%), Postives = 193/460 (41.96%), Query Frame = 0

Query: 3   QVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTAKS 62
           +V +  +N  + +  L    LD+LY +   C A+ R LP LAK +V++ML+++ P+   +
Sbjct: 11  RVHLQCRNLQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAA 70

Query: 63  IEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLPFC 122
           +  WV  +     + +   L  LR++             LNP F+ NL+  L+ G   + 
Sbjct: 71  VALWVKKEFSKAQEESTGLLSGLRIWHTQLLPGGLQGLILNPIFRQNLRIALLGGGKAW- 130

Query: 123 IHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMKVF 182
              D    L           D  S  K +    +  L  ++ S  A    +++  +    
Sbjct: 131 --SDDTSQLGP----DKHARDVPSLDKYAEERWEVVLHFMVGSPSAAVSQDLAQLLS--- 190

Query: 183 QKGLL-SQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLEL 242
           Q GL+ S    E P +T +GFQFL                     R +D  +++SFL +L
Sbjct: 191 QAGLMKSTEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQL 250

Query: 243 SFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLS-------- 302
           SF   G+ Y ++ +++     ++ L + GLV  Q+ RK   + PT+LA NLS        
Sbjct: 251 SFSTLGKDYSVEGMSDSLLNFLQHLREFGLV-FQRKRKSRRYYPTRLAINLSSGVSGAGG 310

Query: 303 -----------------------------------------------------MSLADSS 362
                                                                 ++A   
Sbjct: 311 TVHQPGFIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGI 370

Query: 363 SRKQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFE 381
           + +QI+ FL+  AHP + ++ P +P  +TDQIRLWE + +R+  T    Y++F S+  FE
Sbjct: 371 TAQQIIHFLRTRAHPVMLKQTPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFE 430

BLAST of Spg011675 vs. ExPASy Swiss-Prot
Match: O70422 (General transcription factor IIH subunit 4 OS=Mus musculus OX=10090 GN=Gtf2h4 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.1e-27
Identity = 112/454 (24.67%), Postives = 190/454 (41.85%), Query Frame = 0

Query: 9   KNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTAKSIEEWVL 68
           +N  + +  L    LD+LY +   C A+ R LP LAK +V++ML+++ P+   ++  WV 
Sbjct: 18  RNLQEFLGGLSPGVLDRLYGHPATCLAVFRELPSLAKNWVMRMLFLEQPLPQAAVALWVK 77

Query: 69  PDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLPFCIHQDSA 128
            +     + +   L  LR++             LNP F+ NL+  L+ G   +    D  
Sbjct: 78  KEFSKAQEESTGLLSGLRIWHTQLLPGGLQGLILNPVFRQNLRIALLGGGKAW---SDDT 137

Query: 129 CYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMKVFQKGLL- 188
             L           D  S  K +    +  L  ++ S  A    +++  +    Q GL+ 
Sbjct: 138 SQLGP----DKHARDVPSLDKYAEERWEVVLHFMVGSPSAAVSQDLAQLLS---QAGLMK 197

Query: 189 SQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLELSFHVTG 248
           S    E P +T +GFQFL                     R +D  +++SFL +LSF   G
Sbjct: 198 STEPGEPPCITSAGFQFLLLDTPAQLWYFMLQYLQTAQSRGMDLVEILSFLFQLSFSTLG 257

Query: 249 EAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLS-------------- 308
           + Y ++ +++     ++ L + GLV  Q+ RK   + PT+LA NLS              
Sbjct: 258 KDYSVEGMSDSLLNFLQHLREFGLV-FQRKRKSRRYYPTRLAINLSSGVSGAGGTVHQPG 317

Query: 309 -----------------------------------------------MSLADSSSRKQIV 368
                                                           ++A   + +QI+
Sbjct: 318 FIVVETNYRLYAYTESELQIALIALFSEMLYRFPNMVVAQVTRESVQQAIASGITAQQII 377

Query: 369 TFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEAACDYA 381
            FL+  AHP + ++ P +P  +TDQIRLWE + +R+  T    Y++F S+  FE    +A
Sbjct: 378 HFLRTRAHPVMLKQNPVLPPTITDQIRLWELERDRLRFTEGVLYNQFLSQVDFELLLAHA 437

BLAST of Spg011675 vs. ExPASy Swiss-Prot
Match: Q54C29 (General transcription factor IIH subunit 4 OS=Dictyostelium discoideum OX=44689 GN=gtf2h4 PE=3 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.7e-24
Identity = 112/490 (22.86%), Postives = 195/490 (39.80%), Query Frame = 0

Query: 15  VASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTAKSIEEWVLPDGVSK 74
           +ASL +  L++LY++ + C+AILRSLPP +K+++L+ML +D        ++W     + +
Sbjct: 10  LASLDSKDLEELYKDPWTCQAILRSLPPRSKQYILKMLLVDT-YPLSLAKDWSTQASIQQ 69

Query: 75  YKVAIDRLIQLRVFI---------------------------ETADRKRETTYRLNPTFQ 134
           +K ++ +L  L++                             +   ++ E T RLNP FQ
Sbjct: 70  HKESLKKLFDLKIIFLDKINKPIQPQQQQSSQQSSSQQQQQQQQQQQQTEQTIRLNPLFQ 129

Query: 135 ANLQKLLIHGLLPFCIHQDSACYLFAFIKIQ---VQVDDYSSSIKRSRSWIQCFLLQLIN 194
            N+++ L+        +  S       IK       +DD  S  K     +  FL     
Sbjct: 130 DNIKRSLVQVNQVIFSNNSS-------IKDNHKPPSIDDLDSYSKSQWEKVLYFL----- 189

Query: 195 SGQAEKPSNISSSVMKVFQKGLLSQRDKETPRLTESGFQFLERDV--------------- 254
           S    +PS + S ++       L++++ +   +T  GF+FL +DV               
Sbjct: 190 SDDTVQPSKLISELL---LSSNLTKQEGDGLSITSEGFKFLLKDVYTQIWTLLIVYLDDL 249

Query: 255 ---------DPADLISFLLELSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKD 314
                       DL+SFL  LSF   G  Y +  L+E+Q+  +  L   GL+ +   R D
Sbjct: 250 EKKKGKGSGSRNDLLSFLFRLSFLNLGRGYLVSELSEQQKEYLFALKQFGLIYM---RTD 309

Query: 315 S--WFIPTK----LATNLSMSLADSSSRK------------------------------- 374
           S   F PT+    L T  ++SL  S S +                               
Sbjct: 310 SSILFYPTRLIISLTTGKTLSLIQSISSERTQTQKEQGYIVLETNYRLYAYTSSSLQISL 369

Query: 375 -----------------------------------QIVTFLQQNAHPRVAERIPSVPENV 379
                                              QI+ F++ N+HP  A     +P+ V
Sbjct: 370 LSLFVKMLYRLPNLAVGIITRESIRTALIHGITADQIIDFVRHNSHPNAANSGQPIPDVV 429

BLAST of Spg011675 vs. ExPASy TrEMBL
Match: A0A6J1IIT5 (RNA polymerase II transcription factor B subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111477843 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 336/459 (73.20%), Postives = 348/459 (75.82%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDQLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTY+LNPTFQANLQKLLIHG + 
Sbjct: 61  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYKLNPTFQANLQKLLIHGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++ +       +    +CFLLQLINSGQA+KPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPNLEELEAYALDQWECFLLQLINSGQADKPSNISSSVMK 180

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKETPRLTESGFQFL                    ER VDPADLISFLLE
Sbjct: 181 VFQKGLLSQRDKETPRLTESGFQFLLMETNAQLWYIIREYISNAEERGVDPADLISFLLE 240

Query: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300
           LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS
Sbjct: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300

Query: 301 SRK--------------------------------------------------------- 360
           SRK                                                         
Sbjct: 301 SRKQGFVVVETNFRMYAYSSSKLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGIT 360

Query: 361 --QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 381
             QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA
Sbjct: 361 AQQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 420

BLAST of Spg011675 vs. ExPASy TrEMBL
Match: A0A6J1EGA7 (RNA polymerase II transcription factor B subunit 2 OS=Cucurbita moschata OX=3662 GN=LOC111433104 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 1.0e-170
Identity = 335/459 (72.98%), Postives = 347/459 (75.60%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LY NAFICEAILRSLPPLAKKFVLQMLYIDAPVTA
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDQLYGNAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTY+LNPTFQANLQKLLI G + 
Sbjct: 61  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYKLNPTFQANLQKLLIQGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++ +       +    +CFLLQLINSGQA+KPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPNLEELEAYALDQWECFLLQLINSGQADKPSNISSSVMK 180

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKETPRLTESGFQFL                    ERDVDPADLISFLLE
Sbjct: 181 VFQKGLLSQRDKETPRLTESGFQFLLMETNAQLWYIIREYISNAEERDVDPADLISFLLE 240

Query: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300
           LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS
Sbjct: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300

Query: 301 SRK--------------------------------------------------------- 360
           SRK                                                         
Sbjct: 301 SRKQGFVVVETNFRMYAYSSSKLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGIT 360

Query: 361 --QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 381
             QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA
Sbjct: 361 AQQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 420

BLAST of Spg011675 vs. ExPASy TrEMBL
Match: A0A0A0KK55 (RNA polymerase II transcription factor B subunit 2 OS=Cucumis sativus OX=3659 GN=Csa_6G517270 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 4.0e-170
Identity = 333/459 (72.55%), Postives = 346/459 (75.38%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LYENAFICEAILRSLPPLAKKFVLQMLYID PV+A
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDQLYENAFICEAILRSLPPLAKKFVLQMLYIDGPVSA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHG + 
Sbjct: 61  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++         +    +CFLLQLINSGQAEKPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPSLEDLEAYALDQWECFLLQLINSGQAEKPSNISSSVMK 180

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKE PRLTESGFQFL                    ER VDPADLISFLLE
Sbjct: 181 VFQKGLLSQRDKEAPRLTESGFQFLLMETNAQLWYIIREYISNAEERGVDPADLISFLLE 240

Query: 241 LSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSS 300
           LSFHVTGEAYDIDTL++EQRYAIKDLADLGLVKLQQGRK+SWFIPTKLATNLSMSLADSS
Sbjct: 241 LSFHVTGEAYDIDTLSDEQRYAIKDLADLGLVKLQQGRKESWFIPTKLATNLSMSLADSS 300

Query: 301 SRK--------------------------------------------------------- 360
           SRK                                                         
Sbjct: 301 SRKLGFVVVETNFRMYAYSTSKLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGIT 360

Query: 361 --QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 381
             QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA
Sbjct: 361 AEQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVFEA 420

BLAST of Spg011675 vs. ExPASy TrEMBL
Match: A0A6J1CK16 (RNA polymerase II transcription factor B subunit 2 OS=Momordica charantia OX=3673 GN=LOC111012326 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 9.4e-164
Identity = 334/499 (66.93%), Postives = 344/499 (68.94%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD LYENAFICEAILRSLPPLAKKFVLQMLYI+APV A
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDNLYENAFICEAILRSLPPLAKKFVLQMLYIEAPVAA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVL DGVSKYKVA+DRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLI+G + 
Sbjct: 61  KSMEEWVLSDGVSKYKVALDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIYGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++         +    +CFLLQLINSGQAEKPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPSLEDLEAYALDQWECFLLQLINSGQAEKPSNISSSVMK 180

Query: 181 VFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFLLE 240
           VFQKGLLSQRDKE PRLTESGFQFL                    ER VDPADLISFLLE
Sbjct: 181 VFQKGLLSQRDKEAPRLTESGFQFLLMETNAQLWYIIREYISNSEERGVDPADLISFLLE 240

Query: 241 LSFHVTGE----------------------------------------AYDIDTLTEEQR 300
           LSFHVTGE                                        AYDIDTLTEEQR
Sbjct: 241 LSFHVTGELQWLFGDADLLLRFVCLFRGALFFIVLSGFLYFWEFISFLAYDIDTLTEEQR 300

Query: 301 YAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSSSRK----------------- 360
           YAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSSSRK                 
Sbjct: 301 YAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLADSSSRKQGFVVVETNFRMYAYSS 360

Query: 361 ------------------------------------------QIVTFLQQNAHPRVAERI 381
                                                     QIVTFLQQNAHPRVAERI
Sbjct: 361 SRLHCEILRLFSRIEYQLPNLIVGAITKESLYNAFKNGITAEQIVTFLQQNAHPRVAERI 420

BLAST of Spg011675 vs. ExPASy TrEMBL
Match: A0A5A7UBE2 (RNA polymerase II transcription factor B subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003070 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.2e-155
Identity = 316/466 (67.81%), Postives = 329/466 (70.60%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPAMKLD+LYENAFICEAILRSLPPLAKKFVLQMLYIDAPV+A
Sbjct: 1   MPQVKIIAKNFMDMVASLPAMKLDQLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVSA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGLLP 120
           KS+EEWVLPDGVSKYKVA+DRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHG + 
Sbjct: 61  KSMEEWVLPDGVSKYKVAVDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIHGEVL 120

Query: 121 FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSVMK 180
                 S         I V++         +    +CFLLQLINSGQAEKPSNISSSVMK
Sbjct: 121 AREPMPS--------NITVRLPSLEDLEAYALDQWECFLLQLINSGQAEKPSNISSSVMK 180

Query: 181 VFQKGLLSQ-------------------------RDKETPRLTESGFQFL---------- 240
           VFQKGLLSQ                         RDKE PRLTESGFQFL          
Sbjct: 181 VFQKGLLSQRLKSCSMYSSLYFARYLIKCYVWYSRDKEAPRLTESGFQFLLMETNAQLWY 240

Query: 241 ----------ERDVDPADLISFLLELSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQ 300
                     ER VDPADLISFLLELSFHVTGEAYDIDTL++EQRYAIKDLADLGLVKLQ
Sbjct: 241 IIREYISNAEERGVDPADLISFLLELSFHVTGEAYDIDTLSDEQRYAIKDLADLGLVKLQ 300

Query: 301 QGRKDSWFIPTKLATNLSMSLADSSSRK-------------------------------- 360
           QGRK+SWFIPTKLATNLSMSLADSSSRK                                
Sbjct: 301 QGRKESWFIPTKLATNLSMSLADSSSRKQGFVVVETNFRMYAYSSSKLHCEILRLFSRIE 360

Query: 361 ---------------------------QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWE 363
                                      QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWE
Sbjct: 361 YQLPNLIVGAITKESLYNAFKNGITAEQIVTFLQQNAHPRVAERIPSVPENVTDQIRLWE 420

BLAST of Spg011675 vs. TAIR 10
Match: AT4G17020.2 (transcription factor-related )

HSP 1 Score: 506.5 bits (1303), Expect = 1.8e-143
Identity = 277/460 (60.22%), Postives = 323/460 (70.22%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPA+KLDKLY N FICEAILRSLPPLAKK+VLQMLYID PV A
Sbjct: 1   MPQVKIIAKNFMDMVASLPAIKLDKLYNNVFICEAILRSLPPLAKKYVLQMLYIDVPVPA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIH-GLL 120
             +EEWVL DG SK++VAIDRLIQLR+F E +DRKR T+Y LNPTFQ NLQK +I  G+L
Sbjct: 61  TMMEEWVLADGTSKHRVAIDRLIQLRIFSEISDRKRGTSYSLNPTFQNNLQKHIISGGVL 120

Query: 121 P-FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSV 180
           P   ++ D+A  L +  +++             + W +CFLLQLINSGQ EK + ISSS+
Sbjct: 121 PREPMNSDNAIKLPSLQELETYA---------LKQW-ECFLLQLINSGQGEKLTGISSSM 180

Query: 181 MKVFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFL 240
           MK+FQ+GLLSQRDK+ PRLTESGFQFL                    ERDVDPADLISFL
Sbjct: 181 MKIFQRGLLSQRDKDGPRLTESGFQFLLMDTNAQLWYIIREYILNAEERDVDPADLISFL 240

Query: 241 LELSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLAD 300
           LELSFHVTG+AY+++TLTE Q   +KDLADLGLVKLQQGRKDSWFIPTKLATNLS+SLAD
Sbjct: 241 LELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQQGRKDSWFIPTKLATNLSVSLAD 300

Query: 301 SSSRK------------------------------------------------------- 360
           SS+RK                                                       
Sbjct: 301 SSARKEGFVVMETNFRMYAYSTSKLQCEILRLFARIEYQLPNLIACAITKESLYNAFDNG 360

Query: 361 ----QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVF 380
               QI+TFLQQN+HPR A+R+PS+PENVTDQIRLWE+DL R+++T AHFYDEFPS++VF
Sbjct: 361 ITSDQIITFLQQNSHPRCADRVPSIPENVTDQIRLWETDLQRIEMTQAHFYDEFPSKDVF 420

BLAST of Spg011675 vs. TAIR 10
Match: AT4G17020.1 (transcription factor-related )

HSP 1 Score: 506.5 bits (1303), Expect = 1.8e-143
Identity = 277/460 (60.22%), Postives = 323/460 (70.22%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPA+KLDKLY N FICEAILRSLPPLAKK+VLQMLYID PV A
Sbjct: 1   MPQVKIIAKNFMDMVASLPAIKLDKLYNNVFICEAILRSLPPLAKKYVLQMLYIDVPVPA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIH-GLL 120
             +EEWVL DG SK++VAIDRLIQLR+F E +DRKR T+Y LNPTFQ NLQK +I  G+L
Sbjct: 61  TMMEEWVLADGTSKHRVAIDRLIQLRIFSEISDRKRGTSYSLNPTFQNNLQKHIISGGVL 120

Query: 121 P-FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSV 180
           P   ++ D+A  L +  +++             + W +CFLLQLINSGQ EK + ISSS+
Sbjct: 121 PREPMNSDNAIKLPSLQELETYA---------LKQW-ECFLLQLINSGQGEKLTGISSSM 180

Query: 181 MKVFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFL 240
           MK+FQ+GLLSQRDK+ PRLTESGFQFL                    ERDVDPADLISFL
Sbjct: 181 MKIFQRGLLSQRDKDGPRLTESGFQFLLMDTNAQLWYIIREYILNAEERDVDPADLISFL 240

Query: 241 LELSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLAD 300
           LELSFHVTG+AY+++TLTE Q   +KDLADLGLVKLQQGRKDSWFIPTKLATNLS+SLAD
Sbjct: 241 LELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQQGRKDSWFIPTKLATNLSVSLAD 300

Query: 301 SSSRK------------------------------------------------------- 360
           SS+RK                                                       
Sbjct: 301 SSARKEGFVVMETNFRMYAYSTSKLQCEILRLFARIEYQLPNLIACAITKESLYNAFDNG 360

Query: 361 ----QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVF 380
               QI+TFLQQN+HPR A+R+PS+PENVTDQIRLWE+DL R+++T AHFYDEFPS++VF
Sbjct: 361 ITSDQIITFLQQNSHPRCADRVPSIPENVTDQIRLWETDLQRIEMTQAHFYDEFPSKDVF 420

BLAST of Spg011675 vs. TAIR 10
Match: AT4G17020.3 (transcription factor-related )

HSP 1 Score: 506.5 bits (1303), Expect = 1.8e-143
Identity = 277/460 (60.22%), Postives = 323/460 (70.22%), Query Frame = 0

Query: 1   MPQVKIIAKNFMDMVASLPAMKLDKLYENAFICEAILRSLPPLAKKFVLQMLYIDAPVTA 60
           MPQVKIIAKNFMDMVASLPA+KLDKLY N FICEAILRSLPPLAKK+VLQMLYID PV A
Sbjct: 1   MPQVKIIAKNFMDMVASLPAIKLDKLYNNVFICEAILRSLPPLAKKYVLQMLYIDVPVPA 60

Query: 61  KSIEEWVLPDGVSKYKVAIDRLIQLRVFIETADRKRETTYRLNPTFQANLQKLLIH-GLL 120
             +EEWVL DG SK++VAIDRLIQLR+F E +DRKR T+Y LNPTFQ NLQK +I  G+L
Sbjct: 61  TMMEEWVLADGTSKHRVAIDRLIQLRIFSEISDRKRGTSYSLNPTFQNNLQKHIISGGVL 120

Query: 121 P-FCIHQDSACYLFAFIKIQVQVDDYSSSIKRSRSWIQCFLLQLINSGQAEKPSNISSSV 180
           P   ++ D+A  L +  +++             + W +CFLLQLINSGQ EK + ISSS+
Sbjct: 121 PREPMNSDNAIKLPSLQELETYA---------LKQW-ECFLLQLINSGQGEKLTGISSSM 180

Query: 181 MKVFQKGLLSQRDKETPRLTESGFQFL--------------------ERDVDPADLISFL 240
           MK+FQ+GLLSQRDK+ PRLTESGFQFL                    ERDVDPADLISFL
Sbjct: 181 MKIFQRGLLSQRDKDGPRLTESGFQFLLMDTNAQLWYIIREYILNAEERDVDPADLISFL 240

Query: 241 LELSFHVTGEAYDIDTLTEEQRYAIKDLADLGLVKLQQGRKDSWFIPTKLATNLSMSLAD 300
           LELSFHVTG+AY+++TLTE Q   +KDLADLGLVKLQQGRKDSWFIPTKLATNLS+SLAD
Sbjct: 241 LELSFHVTGQAYNLNTLTEVQNNTLKDLADLGLVKLQQGRKDSWFIPTKLATNLSVSLAD 300

Query: 301 SSSRK------------------------------------------------------- 360
           SS+RK                                                       
Sbjct: 301 SSARKEGFVVMETNFRMYAYSTSKLQCEILRLFARIEYQLPNLIACAITKESLYNAFDNG 360

Query: 361 ----QIVTFLQQNAHPRVAERIPSVPENVTDQIRLWESDLNRVDITPAHFYDEFPSREVF 380
               QI+TFLQQN+HPR A+R+PS+PENVTDQIRLWE+DL R+++T AHFYDEFPS++VF
Sbjct: 361 ITSDQIITFLQQNSHPRCADRVPSIPENVTDQIRLWETDLQRIEMTQAHFYDEFPSKDVF 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023543805.13.9e-17273.42RNA polymerase II transcription factor B subunit 2 [Cucurbita pepo subsp. pepo][more]
KAG6604444.13.9e-17273.42General transcription and DNA repair factor IIH subunit TFB2, partial [Cucurbita... [more]
XP_022977542.12.5e-17173.20RNA polymerase II transcription factor B subunit 2 [Cucurbita maxima][more]
XP_022925808.12.1e-17072.98RNA polymerase II transcription factor B subunit 2 [Cucurbita moschata][more]
XP_004134713.18.2e-17072.55general transcription and DNA repair factor IIH subunit TFB2 isoform X1 [Cucumis... [more]
Match NameE-valueIdentityDescription
Q680U92.6e-14260.22General transcription and DNA repair factor IIH subunit TFB2 OS=Arabidopsis thal... [more]
Q927599.5e-2824.57General transcription factor IIH subunit 4 OS=Homo sapiens OX=9606 GN=GTF2H4 PE=... [more]
P600279.5e-2824.57General transcription factor IIH subunit 4 OS=Pan troglodytes OX=9598 GN=GTF2H4 ... [more]
O704222.1e-2724.67General transcription factor IIH subunit 4 OS=Mus musculus OX=10090 GN=Gtf2h4 PE... [more]
Q54C293.7e-2422.86General transcription factor IIH subunit 4 OS=Dictyostelium discoideum OX=44689 ... [more]
Match NameE-valueIdentityDescription
A0A6J1IIT51.2e-17173.20RNA polymerase II transcription factor B subunit 2 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1EGA71.0e-17072.98RNA polymerase II transcription factor B subunit 2 OS=Cucurbita moschata OX=3662... [more]
A0A0A0KK554.0e-17072.55RNA polymerase II transcription factor B subunit 2 OS=Cucumis sativus OX=3659 GN... [more]
A0A6J1CK169.4e-16466.93RNA polymerase II transcription factor B subunit 2 OS=Momordica charantia OX=367... [more]
A0A5A7UBE21.2e-15567.81RNA polymerase II transcription factor B subunit 2 OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
AT4G17020.21.8e-14360.22transcription factor-related [more]
AT4G17020.11.8e-14360.22transcription factor-related [more]
AT4G17020.31.8e-14360.22transcription factor-related [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.2610coord: 306..380
e-value: 2.5E-27
score: 97.0
IPR004598Transcription factor TFIIH subunit p52/Tfb2PFAMPF03849Tfb2coord: 207..276
e-value: 6.5E-15
score: 54.9
coord: 14..209
e-value: 2.5E-33
score: 115.5
IPR004598Transcription factor TFIIH subunit p52/Tfb2PANTHERPTHR13152TFIIH, POLYPEPTIDE 4coord: 283..378
coord: 5..281
IPR040662Transcription factor Tfb2, C-terminal domainPFAMPF18307Tfb2_Ccoord: 310..377
e-value: 5.2E-23
score: 81.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg011675.1Spg011675.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006289 nucleotide-excision repair
biological_process GO:0070816 phosphorylation of RNA polymerase II C-terminal domain
cellular_component GO:0000439 transcription factor TFIIH core complex
cellular_component GO:0005675 transcription factor TFIIH holo complex
molecular_function GO:0001671 ATPase activator activity
molecular_function GO:0003690 double-stranded DNA binding