Spg011322 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg011322
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionsplicing factor U2af small subunit B-like
Locationscaffold8: 12458019 .. 12459895 (+)
RNA-Seq ExpressionSpg011322
SyntenySpg011322
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGAGCATTTGGCATCCATATTCGGGACGGAGAAGGACAGGGTTAACTGTCCCTTCTACTTCAAGATCGGGGCCTGCCGGCACGGTGATCGGTGTTCGCGGTTGCATACGAAGCCGAGCATAAGCCCCACCCTCTTGCTTTCTAATATGTATCAGAGACCCGACATGATTACTCCTGGTGTCGACGCGCAGGGGAATCCGATTGACCCTCGCGAGATTCAGGACCATTTTGAGGTTTACCTTCTTCTTAATTGCCTTTCTTTTCCTTTTCAATCTTTTCATATGTTCATTACTGTATATTTTTAATATCAATTTTGATATTTTGAGCTGAATCTTGCCTTTCTGGGGTTTGATTGGTTATTATCCGATTGATTTTAGCATAATCTTGCTGTAATTTTAGAGGTAGTCTAACTTGGTTGATTTTTATTTTTTAAGCGATCGTGTTATTTTGAATAGATTCAGATATGGTTTGTTTAACCCTTTTGGTTTCTTAGTTCACTTTATGTTAGTGAAGAATTGTAGCTTAATTGTTGATTTGTGCACATTTCAGGATTTCTATGAAGATTTATTCCAGGAACTGAGCAAGTACGGGGAGATTGAAAGTTTGAATGTTTGTGACAATCTAGCTGACCACATGGTAATACTGCCATCTTCTCCCTTTGTTTGAAGGAAAGCTAATTTTCCAACCGCTTCTCACCTTTGTCATATTTTATTCATTGTAGAATAGCTAATTAGTAGTTATCTGATGCGTTTGATTAGGTGGGTAATGTCTATGTTCAATTTCGAGAGGAAGAACAAGCTGCAAATGCTCTTCGGAATCTGAGTGGCAGATTTTATGCTGGTTAGTTGGTGTTCATGTTTCTTCCCTCTTTCGTCATTCCTTTATTTTTCCCTTTCGTCATTCCTTTATTTTTCCCTTTCGTCATTCTTTCTCTGTTTTTTTTTCACCTTCCCCGTACCATCAATCAACTGTCGTGTTTATACTATTTGTATCCAGTTTTTTCTTTTAAATTTGATGAAATTGGATGTTTAACTTTAACAAATGGAAAACAATTTTTTGCAGGACGTCCAATCATTGTTGACCTAAGAAGCACGGACACGTACACGGGACACGGACACGGACACGACAGGACACGGCGATACGTCAAATTCTAAAAAACTAAGATACGGACACGTTGAAGATACGTTGTTTTATATATATATATATATATATATATATTTTTAAAAAAATTTAGTATATATATAGATTAGTTTTTTTAAAAAAATAGTATATATATAAAACTTTGTTGAGAGTAGTTTAATAATCTTTCATTTTTTTTTTAATTTAAAAACAGTTTTAGTTTTTTATTGGGCTGGGCCTTTTATCTAGGTTAACTTTTTAGGTTTTAAAAGATAAAAAAAAAAAAAGAAAACTTAGATGAACCAGTGGGTTATTGGGCTGGGCCTTTTATCTAGGTTAACTTTTTAGGTTTTAAAAGATAAAAGAAGAAAAACCAGTGGGTGAAGGGAGATATTGGAGGCAACTTCCGGCGGCAGCATGTTGAGGTTTTTGTGCTCTCTCTCTCTCTCTCTCTAGACTCAGAGCGTGTCCGAATGAAAGAGATGCTTATCATGAAAGTCGGAGCAGGAGGCATAGGGGTACAAGCCCTGGTCGTAGAAGAAGCAGAAGTCGTAGTCCACGAGGAAGGAAGAATCGAAATCCAGTTAGAGAAGGGAGTGAAGAGAGGCGTGCTAAAATTGAGCAGTGGAACAGGGAAAGAGAACAAGCAGCAAATGATAACAATGCCAATTCTGGTAGCAACAAAAATAGCCATGAAAGAAGTTACAATGGTGATGTAAAGTATGCAAATCAGGCCTGTGGTTATGACTTATGA

mRNA sequence

ATGGCAGAGCATTTGGCATCCATATTCGGGACGGAGAAGGACAGGGTTAACTGTCCCTTCTACTTCAAGATCGGGGCCTGCCGGCACGGTGATCGGTGTTCGCGGTTGCATACGAAGCCGAGCATAAGCCCCACCCTCTTGCTTTCTAATATGTATCAGAGACCCGACATGATTACTCCTGGTGTCGACGCGCAGGGGAATCCGATTGACCCTCGCGAGATTCAGGACCATTTTGAGGATTTCTATGAAGATTTATTCCAGGAACTGAGCAAGTACGGGGAGATTGAAAGTTTGAATGTTTGTGACAATCTAGCTGACCACATGGTGGGTAATGTCTATGTTCAATTTCGAGAGGAAGAACAAGCTGCAAATGCTCTTCGGAATCTGAGTGGCAGATTTTATGCTGGACGTCCAATCATTGTTGACCTAAGAAGCACGGACACACTCAGAGCGTGTCCGAATGAAAGAGATGCTTATCATGAAAGTCGGAGCAGGAGGCATAGGGGTACAAGCCCTGGTCGTAGAAGAAGCAGAAGTCGTAGTCCACGAGGAAGGAAGAATCGAAATCCAGTTAGAGAAGGGAGTGAAGAGAGGCGTGCTAAAATTGAGCAGTGGAACAGGGAAAGAGAACAAGCAGCAAATGATAACAATGCCAATTCTGGTAGCAACAAAAATAGCCATGAAAGAAGTTACAATGGTGATGTAAAGTATGCAAATCAGGCCTGTGGTTATGACTTATGA

Coding sequence (CDS)

ATGGCAGAGCATTTGGCATCCATATTCGGGACGGAGAAGGACAGGGTTAACTGTCCCTTCTACTTCAAGATCGGGGCCTGCCGGCACGGTGATCGGTGTTCGCGGTTGCATACGAAGCCGAGCATAAGCCCCACCCTCTTGCTTTCTAATATGTATCAGAGACCCGACATGATTACTCCTGGTGTCGACGCGCAGGGGAATCCGATTGACCCTCGCGAGATTCAGGACCATTTTGAGGATTTCTATGAAGATTTATTCCAGGAACTGAGCAAGTACGGGGAGATTGAAAGTTTGAATGTTTGTGACAATCTAGCTGACCACATGGTGGGTAATGTCTATGTTCAATTTCGAGAGGAAGAACAAGCTGCAAATGCTCTTCGGAATCTGAGTGGCAGATTTTATGCTGGACGTCCAATCATTGTTGACCTAAGAAGCACGGACACACTCAGAGCGTGTCCGAATGAAAGAGATGCTTATCATGAAAGTCGGAGCAGGAGGCATAGGGGTACAAGCCCTGGTCGTAGAAGAAGCAGAAGTCGTAGTCCACGAGGAAGGAAGAATCGAAATCCAGTTAGAGAAGGGAGTGAAGAGAGGCGTGCTAAAATTGAGCAGTGGAACAGGGAAAGAGAACAAGCAGCAAATGATAACAATGCCAATTCTGGTAGCAACAAAAATAGCCATGAAAGAAGTTACAATGGTGATGTAAAGTATGCAAATCAGGCCTGTGGTTATGACTTATGA

Protein sequence

MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEEQAANALRNLSGRFYAGRPIIVDLRSTDTLRACPNERDAYHESRSRRHRGTSPGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSYNGDVKYANQACGYDL
Homology
BLAST of Spg011322 vs. NCBI nr
Match: XP_023519661.1 (splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023519662.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023519664.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 395.6 bits (1015), Expect = 3.2e-106
Identity = 212/313 (67.73%), Postives = 224/313 (71.57%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR+IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYH+SR+RRHR TS
Sbjct: 181 RHELFASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 245
           PG R SRSRSPRGRKNR+PVREGSEERRAKIEQWNRE+EQ  N NNANS  N+NSHE+SY
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQQENYNNANSDGNENSHEKSY 300

BLAST of Spg011322 vs. NCBI nr
Match: XP_004139466.1 (splicing factor U2af small subunit B [Cucumis sativus] >XP_011654262.1 splicing factor U2af small subunit B [Cucumis sativus] >KGN64894.1 hypothetical protein Csa_022738 [Cucumis sativus])

HSP 1 Score: 392.5 bits (1007), Expect = 2.7e-105
Identity = 214/314 (68.15%), Postives = 225/314 (71.66%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYHESRSRRHR TS
Sbjct: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 246
           PG  RSRSRSPRGRKNR+PVREGSEERRAKIEQWN+EREQ  NDNNANS  N+N+HE+SY
Sbjct: 241 PG-HRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQ-GNDNNANSDDNRNNHEKSY 300

BLAST of Spg011322 vs. NCBI nr
Match: XP_022964867.1 (splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964868.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964870.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >KAG7019309.1 Splicing factor U2af small subunit A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.5 bits (1007), Expect = 2.7e-105
Identity = 210/313 (67.09%), Postives = 224/313 (71.57%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR+IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYH+SR+RRHR TS
Sbjct: 181 RHELFASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 245
           PG R SRSRSPRGRKNR+PVREGSEERRAKIEQWNRE+EQ  N NN+NS  ++NSHE+SY
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQQKNYNNSNSDGHENSHEKSY 300

BLAST of Spg011322 vs. NCBI nr
Match: XP_008461981.1 (PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_008461988.1 PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >KAA0065889.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa] >TYK15128.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 387.1 bits (993), Expect = 1.1e-103
Identity = 212/314 (67.52%), Postives = 223/314 (71.02%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR+IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYHE+RSRR R TS
Sbjct: 181 RHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 246
           PG  RSRSRSPRGRKNR+P REGSEERRAKIEQWNREREQ  NDNNA S  N+NSHE+SY
Sbjct: 241 PG-HRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQ-GNDNNAKSDDNRNSHEKSY 300

BLAST of Spg011322 vs. NCBI nr
Match: XP_022970323.1 (splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970325.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970326.1 splicing factor U2af small subunit B-like [Cucurbita maxima])

HSP 1 Score: 386.3 bits (991), Expect = 1.9e-103
Identity = 210/313 (67.09%), Postives = 223/313 (71.25%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR+IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYH+SR+RRHR TS
Sbjct: 181 RHELFASYHRRRGHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 245
           PG R SRSRSPRGRKNR+PVRE SEERRAKIEQWNRE+EQ  N NNANS  N++SHE+SY
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREESEERRAKIEQWNREKEQ-ENYNNANSDGNEHSHEKSY 300

BLAST of Spg011322 vs. ExPASy Swiss-Prot
Match: Q9S709 (Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 9.0e-72
Identity = 153/290 (52.76%), Postives = 174/290 (60.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG P+DPR+IQ+HFEDF+EDLF+EL K+GEIESLN+CDNLADHM+GNVYVQF+EE+
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDL------------------------------------- 180
           QAA AL+ L GRFY+GRPII D                                      
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 ----------------------------RSTDTLRACPNERDAYHESRSRRHRGTSPGRR 212
                                          D  R  P+ R+  H  R R       G+R
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKR 240

BLAST of Spg011322 vs. ExPASy Swiss-Prot
Match: Q9ZQW8 (Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35A PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 1.5e-71
Identity = 158/288 (54.86%), Postives = 176/288 (61.11%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH KPS+SPTLLLSNMY RPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNKPSVSPTLLLSNMYLRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           G+DAQGNPIDP +IQ  FEDFYED+F+ELSKYGEIESL+VCDN ADHM+GNVYVQFREE+
Sbjct: 61  GIDAQGNPIDPEKIQADFEDFYEDIFEELSKYGEIESLHVCDNFADHMIGNVYVQFREED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAA AL+ L+GR+Y+GRPIIV+       R                              
Sbjct: 121 QAARALQALTGRYYSGRPIIVEFSPVSDFREATCRQYEENSCNRGGYCNFMHVKEIGRDL 180

Query: 181 -----------------------------NERDAYHESRSRRH----RGTS-----PGRR 213
                                         + D    SRSR H    RG S      G R
Sbjct: 181 RKRLFGHLHRSRRSHSHGRSRSPSPYHYRRDYDRRSSSRSRDHDDYYRGGSHDYYRGGSR 240

BLAST of Spg011322 vs. ExPASy Swiss-Prot
Match: Q9FMY5 (Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B PE=1 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.0e-71
Identity = 149/281 (53.02%), Postives = 171/281 (60.85%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP +IQDHFEDFYED+F+EL+K+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
            AA AL+ L GRFY+GRPII D       R                              
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 ------NERDAYHESRSRRHRGTSPGRRRSRSR--------------------------- 212
                   R +Y      R R  SP R+R  SR                           
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

BLAST of Spg011322 vs. ExPASy Swiss-Prot
Match: Q6AUG0 (Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35B PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 3.5e-68
Identity = 152/302 (50.33%), Postives = 175/302 (57.95%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P++SPT++L+NMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTVSPTIVLANMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG PIDP ++Q+HFEDFYED+++ELSK+GE+E+LNVCDNLADHM+GNVYVQFREEE
Sbjct: 61  GVDAQGQPIDPEKMQEHFEDFYEDIYEELSKFGEVETLNVCDNLADHMIGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QA  A   L GRFY+GRPIIV+       R                              
Sbjct: 121 QAVAAHNALQGRFYSGRPIIVEYSPVTDFREATCRQFEENSCNRGGYCNFMHVKQIGREL 180

Query: 181 -------NERDAYHESR--SRRHR------------------------------------ 212
                    R ++  SR  S RHR                                    
Sbjct: 181 RRKLYGGRSRRSHGRSRSPSPRHRRGNRDRDDFRRERDGYRGGGDGYRGGGGGGGGDGYR 240

BLAST of Spg011322 vs. ExPASy Swiss-Prot
Match: Q94535 (Splicing factor U2af 38 kDa subunit OS=Drosophila melanogaster OX=7227 GN=U2af38 PE=1 SV=2)

HSP 1 Score: 183.0 bits (463), Expect = 4.2e-45
Identity = 103/217 (47.47%), Postives = 128/217 (58.99%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAE+LASIFGTEKD+VNC FYFKIGACRHGDRCSR+H KP+ S T+LL N+Y  P     
Sbjct: 1   MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRIHNKPTFSQTVLLQNLYVNPQNSAK 60

Query: 61  GVDAQG--NPIDPREIQDHFEDFYEDLFQEL-SKYGEIESLNVCDNLADHMVGNVYVQFR 120
             D       +   E+Q+H+++F+ED+F E   KYGEIE +NVCDNL DH+VGNVY++FR
Sbjct: 61  SADGSHLVANVSDEEMQEHYDNFFEDVFVECEDKYGEIEEMNVCDNLGDHLVGNVYIKFR 120

Query: 121 EEEQAANALRNLSGRFYAGRPIIVDLRS-TDTLRACPNE--------------------- 180
            E  A  A  +L+ R++ GRP+  +L   TD   AC  +                     
Sbjct: 121 NEADAEKAANDLNNRWFGGRPVYSELSPVTDFREACCRQYEMGECTRSGFCNFMHLKPIS 180

Query: 181 ----RDAYHESRSRRHRGTSPGRR---RSRSRSPRGR 186
               R  Y   R  R R  SPGRR   RSRSRSP  R
Sbjct: 181 RELRRYLYSRRRRARSRSRSPGRRRGSRSRSRSPGRR 217

BLAST of Spg011322 vs. ExPASy TrEMBL
Match: A0A6J1HM65 (splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111464850 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.3e-105
Identity = 210/313 (67.09%), Postives = 224/313 (71.57%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR+IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYH+SR+RRHR TS
Sbjct: 181 RHELFASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 245
           PG R SRSRSPRGRKNR+PVREGSEERRAKIEQWNRE+EQ  N NN+NS  ++NSHE+SY
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQQKNYNNSNSDGHENSHEKSY 300

BLAST of Spg011322 vs. ExPASy TrEMBL
Match: A0A0A0LVA4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G145960 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.3e-105
Identity = 214/314 (68.15%), Postives = 225/314 (71.66%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRNIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYHESRSRRHR TS
Sbjct: 181 RHELFAMYRRRHSHSRSRSRSPYRHRSYEERSYGKHGHSRRYDERDAYHESRSRRHRTTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 246
           PG  RSRSRSPRGRKNR+PVREGSEERRAKIEQWN+EREQ  NDNNANS  N+N+HE+SY
Sbjct: 241 PG-HRSRSRSPRGRKNRSPVREGSEERRAKIEQWNKEREQ-GNDNNANSDDNRNNHEKSY 300

BLAST of Spg011322 vs. ExPASy TrEMBL
Match: A0A1S3CFU2 (splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC103500459 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 5.4e-104
Identity = 212/314 (67.52%), Postives = 223/314 (71.02%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR+IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYHE+RSRR R TS
Sbjct: 181 RHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 246
           PG  RSRSRSPRGRKNR+P REGSEERRAKIEQWNREREQ  NDNNA S  N+NSHE+SY
Sbjct: 241 PG-HRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQ-GNDNNAKSDDNRNSHEKSY 300

BLAST of Spg011322 vs. ExPASy TrEMBL
Match: A0A5D3CV58 (Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold259G00620 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 5.4e-104
Identity = 212/314 (67.52%), Postives = 223/314 (71.02%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR+IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQFREEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYHE+RSRR R TS
Sbjct: 181 RHELFAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 246
           PG  RSRSRSPRGRKNR+P REGSEERRAKIEQWNREREQ  NDNNA S  N+NSHE+SY
Sbjct: 241 PG-HRSRSRSPRGRKNRSPAREGSEERRAKIEQWNREREQ-GNDNNAKSDDNRNSHEKSY 300

BLAST of Spg011322 vs. ExPASy TrEMBL
Match: A0A6J1I2J7 (splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC111469321 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 9.3e-104
Identity = 210/313 (67.09%), Postives = 223/313 (71.25%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQGNPIDPR+IQDHFE+FYEDLFQEL+KYGEIESLNVCDNLADHMVGNVYVQF+EEE
Sbjct: 61  GVDAQGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEE 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
           QAANALRNLSGRFYAGRPIIVD       R                              
Sbjct: 121 QAANALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISREL 180

Query: 181 ------------------------------------------NERDAYHESRSRRHRGTS 240
                                                     +ERDAYH+SR+RRHR TS
Sbjct: 181 RHELFASYHRRRGHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTS 240

Query: 241 PGRRRSRSRSPRGRKNRNPVREGSEERRAKIEQWNREREQAANDNNANSGSNKNSHERSY 245
           PG R SRSRSPRGRKNR+PVRE SEERRAKIEQWNRE+EQ  N NNANS  N++SHE+SY
Sbjct: 241 PGHRGSRSRSPRGRKNRSPVREESEERRAKIEQWNREKEQ-ENYNNANSDGNEHSHEKSY 300

BLAST of Spg011322 vs. TAIR 10
Match: AT1G27650.1 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 271.6 bits (693), Expect = 6.4e-73
Identity = 153/290 (52.76%), Postives = 174/290 (60.00%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVDAQG P+DPR+IQ+HFEDF+EDLF+EL K+GEIESLN+CDNLADHM+GNVYVQF+EE+
Sbjct: 61  GVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDL------------------------------------- 180
           QAA AL+ L GRFY+GRPII D                                      
Sbjct: 121 QAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSREL 180

Query: 181 ----------------------------RSTDTLRACPNERDAYHESRSRRHRGTSPGRR 212
                                          D  R  P+ R+  H  R R       G+R
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKR 240

BLAST of Spg011322 vs. TAIR 10
Match: AT5G42820.2 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 270.4 bits (690), Expect = 1.4e-72
Identity = 149/281 (53.02%), Postives = 171/281 (60.85%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP +IQDHFEDFYED+F+EL+K+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
            AA AL+ L GRFY+GRPII D       R                              
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 ------NERDAYHESRSRRHRGTSPGRRRSRSR--------------------------- 212
                   R +Y      R R  SP R+R  SR                           
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

BLAST of Spg011322 vs. TAIR 10
Match: AT5G42820.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 270.4 bits (690), Expect = 1.4e-72
Identity = 149/281 (53.02%), Postives = 171/281 (60.85%), Query Frame = 0

Query: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITP 60
           MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLH +P+ISPTLLLSNMYQRPDMITP
Sbjct: 1   MAEHLASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITP 60

Query: 61  GVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEE 120
           GVD QG P+DP +IQDHFEDFYED+F+EL+K+GE+ESLNVCDNLADHM+GNVYV F+EE+
Sbjct: 61  GVDPQGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEED 120

Query: 121 QAANALRNLSGRFYAGRPIIVDLRSTDTLRACP--------------------------- 180
            AA AL+ L GRFY+GRPII D       R                              
Sbjct: 121 HAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISREL 180

Query: 181 ------NERDAYHESRSRRHRGTSPGRRRSRSR--------------------------- 212
                   R +Y      R R  SP R+R  SR                           
Sbjct: 181 RRKLFGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD 240

BLAST of Spg011322 vs. TAIR 10
Match: AT1G27650.2 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 167.9 bits (424), Expect = 1.0e-41
Identity = 106/240 (44.17%), Postives = 125/240 (52.08%), Query Frame = 0

Query: 51  MYQRPDMITPGVDAQGNPIDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVG 110
           MYQRPDMITPGVDAQG P+DPR+IQ+HFEDF+EDLF+EL K+GEIESLN+CDNLADHM+G
Sbjct: 1   MYQRPDMITPGVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIG 60

Query: 111 NVYVQFREEEQAANALRNLSGRFYAGRPIIVDL--------------------------- 170
           NVYVQF+EE+QAA AL+ L GRFY+GRPII D                            
Sbjct: 61  NVYVQFKEEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF 120

Query: 171 --------------------------------------RSTDTLRACPNERDAYHESRSR 212
                                                    D  R  P+ R+  H  R R
Sbjct: 121 MHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDR 180

BLAST of Spg011322 vs. TAIR 10
Match: AT1G10320.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 121.7 bits (304), Expect = 8.2e-28
Identity = 60/131 (45.80%), Postives = 85/131 (64.89%), Query Frame = 0

Query: 9   FGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDAQGNP 68
           FGTE+D+ +CPF+ K GACR G RCSR+H  P+ S TLL+ NMY  P  IT   D +G  
Sbjct: 237 FGTEQDKAHCPFHLKTGACRFGQRCSRVHFYPNKSCTLLMKNMYNGPG-ITWEQD-EGLE 296

Query: 69  IDPREIQDHFEDFYEDLFQELSKYGEIESLNVCDNLADHMVGNVYVQFREEEQAANALRN 128
               E +  +E+FYED+  E  KYGE+ +  VC N + H+ GNVYV +R  E A  A ++
Sbjct: 297 YTDEEAELCYEEFYEDVHTEFLKYGELVNFKVCRNGSFHLKGNVYVHYRSLESAILAYQS 356

Query: 129 LSGRFYAGRPI 140
           ++GR++AG+ +
Sbjct: 357 INGRYFAGKQV 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023519661.13.2e-10667.73splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_02351... [more]
XP_004139466.12.7e-10568.15splicing factor U2af small subunit B [Cucumis sativus] >XP_011654262.1 splicing ... [more]
XP_022964867.12.7e-10567.09splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964868.1 s... [more]
XP_008461981.11.1e-10367.52PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_00846198... [more]
XP_022970323.11.9e-10367.09splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970325.1 spl... [more]
Match NameE-valueIdentityDescription
Q9S7099.0e-7252.76Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A ... [more]
Q9ZQW81.5e-7154.86Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9FMY52.0e-7153.02Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B ... [more]
Q6AUG03.5e-6850.33Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q945354.2e-4547.47Splicing factor U2af 38 kDa subunit OS=Drosophila melanogaster OX=7227 GN=U2af38... [more]
Match NameE-valueIdentityDescription
A0A6J1HM651.3e-10567.09splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A0A0LVA41.3e-10568.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G145960 PE=4 SV=1[more]
A0A1S3CFU25.4e-10467.52splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC10350045... [more]
A0A5D3CV585.4e-10467.52Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1I2J79.3e-10467.09splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT1G27650.16.4e-7352.76U2 snRNP auxiliary factor small subunit, putative [more]
AT5G42820.21.4e-7253.02Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
AT5G42820.11.4e-7253.02Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
AT1G27650.21.0e-4144.17U2 snRNP auxiliary factor small subunit, putative [more]
AT1G10320.18.2e-2845.80Zinc finger C-x8-C-x5-C-x3-H type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 195..215
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 211..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..210
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 154..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 167..185
NoneNo IPR availablePANTHERPTHR12620:SF39SPLICING FACTOR U2AF SMALL SUBUNIT Acoord: 1..148
coord: 153..240
NoneNo IPR availableCDDcd12539RRM_U2AF35Bcoord: 44..142
e-value: 4.0546E-64
score: 193.349
IPR009145U2 auxiliary factor small subunitPRINTSPR01848U2AUXFACTORcoord: 37..57
score: 57.14
coord: 75..90
score: 53.41
coord: 103..125
score: 65.42
coord: 130..154
score: 30.55
coord: 18..37
score: 85.68
IPR009145U2 auxiliary factor small subunitPANTHERPTHR12620U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNITcoord: 1..148
coord: 153..240
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 13..39
e-value: 0.0065
score: 25.7
IPR000571Zinc finger, CCCH-typePFAMPF00642zf-CCCHcoord: 14..37
e-value: 3.6E-7
score: 29.9
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 12..40
score: 13.112443
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 45..142
e-value: 3.1E-4
score: 30.1
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 83..139
e-value: 1.9E-7
score: 30.8
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 44..146
score: 11.980452
IPR003954RNA recognition motif domain, eukaryoteSMARTSM00361rrm2_1coord: 73..142
e-value: 1.2E-5
score: 34.7
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 43..144
e-value: 3.6E-30
score: 106.3
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 77..145

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg011322.1Spg011322.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005681 spliceosomal complex
cellular_component GO:0089701 U2AF complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030628 pre-mRNA 3'-splice site binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003723 RNA binding