Spg011064 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg011064
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	E3 ubiquitin-protein ligase RNF181-like
Location	scaffold4: 26602536 .. 26603111 (+)
RNA-Seq Expression	Spg011064
Synteny	Spg011064

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCCGAAGAACCCAATTCCCCTGAAATTTCCTCTGTGTTGGAAACCCTAGTCGGAAACAGAGACCTCTCTCTCTTCATGCCCTTCCTCTTCGGCTTCTCCAACAGAGACCCAAATCCCCAAAATGAAAATCGGAGCGAGAGATTCTTTCTGGTGAACCCTTTCACTCAGAGCATGGTTCTGATTGAAGGGAGTTCGAGCTTGGAAGCGCTTCTGCTTGATTTCGGTGGGAAGGAAGGGCAGCCACCAGCCTCCAAAGCCTCCATAGAAGCGCTTCCCACTGTTCGACTCATGGAGGAGGAATTGCCTTGCGAGTGCGTCATCTGTTTGGATGAGTGGGAAATTGGGGCTGTGGCTAAACAATTGCCTTGCAAACACAAATTCCATCCCAATTGTATAGAGAAGTGGCTTGGGATTCATGGCAACTGTCCTGTTTGTAGGTACCAATTGCCTGTTGATGGGAAGAAAAACGACGTCGGACTGAGAGCAAGGAGTGTGAGGGAGATTTGGGTCGGTTTTTCTTTGAATAATGATAGGAGAAGTGATGATTCTAATCGAGTTTCTTCGGATTAG

mRNA sequence

ATGGCCTCCGAAGAACCCAATTCCCCTGAAATTTCCTCTGTGTTGGAAACCCTAGTCGGAAACAGAGACCTCTCTCTCTTCATGCCCTTCCTCTTCGGCTTCTCCAACAGAGACCCAAATCCCCAAAATGAAAATCGGAGCGAGAGATTCTTTCTGGTGAACCCTTTCACTCAGAGCATGGTTCTGATTGAAGGGAGTTCGAGCTTGGAAGCGCTTCTGCTTGATTTCGGTGGGAAGGAAGGGCAGCCACCAGCCTCCAAAGCCTCCATAGAAGCGCTTCCCACTGTTCGACTCATGGAGGAGGAATTGCCTTGCGAGTGCGTCATCTGTTTGGATGAGTGGGAAATTGGGGCTGTGGCTAAACAATTGCCTTGCAAACACAAATTCCATCCCAATTGTATAGAGAAGTGGCTTGGGATTCATGGCAACTGTCCTGTTTGTAGGTACCAATTGCCTGTTGATGGGAAGAAAAACGACGTCGGACTGAGAGCAAGGAGTGTGAGGGAGATTTGGGTCGGTTTTTCTTTGAATAATGATAGGAGAAGTGATGATTCTAATCGAGTTTCTTCGGATTAG

Coding sequence (CDS)

ATGGCCTCCGAAGAACCCAATTCCCCTGAAATTTCCTCTGTGTTGGAAACCCTAGTCGGAAACAGAGACCTCTCTCTCTTCATGCCCTTCCTCTTCGGCTTCTCCAACAGAGACCCAAATCCCCAAAATGAAAATCGGAGCGAGAGATTCTTTCTGGTGAACCCTTTCACTCAGAGCATGGTTCTGATTGAAGGGAGTTCGAGCTTGGAAGCGCTTCTGCTTGATTTCGGTGGGAAGGAAGGGCAGCCACCAGCCTCCAAAGCCTCCATAGAAGCGCTTCCCACTGTTCGACTCATGGAGGAGGAATTGCCTTGCGAGTGCGTCATCTGTTTGGATGAGTGGGAAATTGGGGCTGTGGCTAAACAATTGCCTTGCAAACACAAATTCCATCCCAATTGTATAGAGAAGTGGCTTGGGATTCATGGCAACTGTCCTGTTTGTAGGTACCAATTGCCTGTTGATGGGAAGAAAAACGACGTCGGACTGAGAGCAAGGAGTGTGAGGGAGATTTGGGTCGGTTTTTCTTTGAATAATGATAGGAGAAGTGATGATTCTAATCGAGTTTCTTCGGATTAG

Protein sequence

MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNRDPNPQNENRSERFFLVNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVICLDEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVDGKKNDVGLRARSVREIWVGFSLNNDRRSDDSNRVSSD

Homology

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BLAST of Spg011064 vs. NCBI nr
Match: XP_023523370.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 293.5 bits (750), Expect = 1.3e-75
Identity = 149/202 (73.76%), Postives = 167/202 (82.67%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNRDP-----NP-----QNENRSERF 60
           MAS+  NSPEISSV +TL+GNRDLSLFMPFLFGFS  DP     NP     QN NRS+R 
Sbjct: 38  MASDS-NSPEISSVFDTLIGNRDLSLFMPFLFGFSAADPLQQSQNPADPDRQNANRSDRI 97

Query: 61  FLVNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVIC 120
           FLVNPFTQSMV+IEG SSLEAL+ D GGK+GQPPAS+ASIEALPTV++M EEL CECVIC
Sbjct: 98  FLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEELGCECVIC 157

Query: 121 LDEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVD-GKKNDVGLRARSVRE 180
           LDEWEIGAVAK+LPC HKFHP+CIEKWLG+HGNCP+CR +LP D  +KN+ G RA   RE
Sbjct: 158 LDEWEIGAVAKELPCGHKFHPDCIEKWLGVHGNCPLCRCKLPDDVDRKNNEGRRA---RE 217

Query: 181 IWVGFSLNNDRRSDDSNRVSSD 192
           IWVGFS NNDRRS+ SN VSSD
Sbjct: 218 IWVGFSFNNDRRSEGSNGVSSD 235

[Close]

BLAST of Spg011064 vs. NCBI nr
Match: XP_022997776.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima])

HSP 1 Score: 292.7 bits (748), Expect = 2.2e-75
Identity = 148/202 (73.27%), Postives = 166/202 (82.18%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNRDP-----NP-----QNENRSERF 60
           MAS+  NSPEISSV +T +GNRDLSLFMPFLFGFS  DP     NP     QN NRS+R 
Sbjct: 38  MASDS-NSPEISSVFDTFIGNRDLSLFMPFLFGFSTADPLQQSQNPADPDRQNANRSDRI 97

Query: 61  FLVNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVIC 120
           FLVNPFTQSMV+IEG SSLEAL+ D GGK+GQPPAS+ASIEALPTV++M EEL CECVIC
Sbjct: 98  FLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEELGCECVIC 157

Query: 121 LDEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVD-GKKNDVGLRARSVRE 180
           LDEWEIGAVAK+LPC HKFHP+CIEKWLG+HGNCP+CR +LP D  +KN+ G RA   RE
Sbjct: 158 LDEWEIGAVAKELPCGHKFHPDCIEKWLGVHGNCPLCRCKLPDDVDRKNNEGRRA---RE 217

Query: 181 IWVGFSLNNDRRSDDSNRVSSD 192
           IWVGFS NNDRRS+ SN VSSD
Sbjct: 218 IWVGFSFNNDRRSEGSNGVSSD 235

[Close]

BLAST of Spg011064 vs. NCBI nr
Match: XP_022949551.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita moschata])

HSP 1 Score: 287.3 bits (734), Expect = 9.4e-74
Identity = 147/202 (72.77%), Postives = 165/202 (81.68%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNRDP-----NP-----QNENRSERF 60
           MAS+  NSPEISSV +TL+GNRDLSLFMP LFGFS  DP     NP     QN NRS+R 
Sbjct: 1   MASDS-NSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRI 60

Query: 61  FLVNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVIC 120
           FLVNPFTQSMV+IEG SSLEAL+ D GGK+GQPPAS+ASIEALPTV++M EE  CECVIC
Sbjct: 61  FLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVIC 120

Query: 121 LDEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVD-GKKNDVGLRARSVRE 180
           LDEW+IGAVAK+LPC HKFHP+CIEKWLGIHGNCP+CR +LP D  +KN+ G RA   RE
Sbjct: 121 LDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRA---RE 180

Query: 181 IWVGFSLNNDRRSDDSNRVSSD 192
           IWVGFS NNDRRS+ SN VSSD
Sbjct: 181 IWVGFSFNNDRRSEGSNGVSSD 198

[Close]

BLAST of Spg011064 vs. NCBI nr
Match: KAG6607122.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036814.1 E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 287.3 bits (734), Expect = 9.4e-74
Identity = 147/202 (72.77%), Postives = 164/202 (81.19%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNRDP-----NP-----QNENRSERF 60
           MAS+  NSPE SSV +TL+GNRDLSLFMPFLFGFS  DP     NP     QN NRS+R 
Sbjct: 1   MASDS-NSPETSSVFDTLIGNRDLSLFMPFLFGFSAADPLQQSQNPADPDRQNANRSDRI 60

Query: 61  FLVNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVIC 120
           FLVNPFTQSMV+IEG SSLEAL+ D GGK+GQPPAS+ASIEALPTV++M EE  CECVIC
Sbjct: 61  FLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVIC 120

Query: 121 LDEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVD-GKKNDVGLRARSVRE 180
           LDEWEIGAVAK+LPC HKFHP+CIEKWLGIHGNCP+CR +LP D  +KN+ G RA   RE
Sbjct: 121 LDEWEIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRA---RE 180

Query: 181 IWVGFSLNNDRRSDDSNRVSSD 192
           IWVGFS  NDRRS+ SN VSSD
Sbjct: 181 IWVGFSFTNDRRSEGSNGVSSD 198

[Close]

BLAST of Spg011064 vs. NCBI nr
Match: XP_022156648.1 (E3 ubiquitin-protein ligase RNF181 [Momordica charantia])

HSP 1 Score: 262.3 bits (669), Expect = 3.2e-66
Identity = 135/200 (67.50%), Postives = 152/200 (76.00%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNR---DPNPQNEN----RSERFFLV 60
           MAS EP SPE SS          L  F PFLFGF+N    DPN ++ N     SERFFLV
Sbjct: 1   MASSEPESPETSS----------LFAFTPFLFGFTNNRRDDPNQESRNPANRESERFFLV 60

Query: 61  NPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVICLDE 120
           NPFTQSMV+IEGSS+LE+LL D G K+G PPASKASIEALP V+L+EE    ECVICLDE
Sbjct: 61  NPFTQSMVVIEGSSALESLLRDLGSKDGPPPASKASIEALPAVQLLEEG---ECVICLDE 120

Query: 121 WEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPV---DGKKNDVGLRARSVREI 180
           WEIGA AK+LPCKHKFHPNCIEKWLGIHG+CP+CR+++PV   DGKKNDV  R R +REI
Sbjct: 121 WEIGATAKELPCKHKFHPNCIEKWLGIHGSCPICRHKMPVDDGDGKKNDVVGRGRRLREI 180

Query: 181 WVGFSLNNDRRSDDSNRVSS 191
           WVGFS NND RSDDSN + S
Sbjct: 181 WVGFSFNNDIRSDDSNPLPS 187

[Close]

BLAST of Spg011064 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.5e-45
Identity = 90/181 (49.72%), Postives = 122/181 (67.40%), Query Frame = 0


Query: 10  EISSVLETLVGNRDLSLFMPFLFGFSNRDPNPQNE----NRSERFFLVNPFTQSMVLIEG 69
           E SSV    + NRDL LF+PFL GFS+++ +  ++    +  ER  LVNPFTQ M+++EG
Sbjct: 12  ETSSVSGRFLRNRDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEG 71

Query: 70  SSSLEALLLDF--GGKEGQPPASKASIEALPTVRLMEEELPCECVICLDEWEIGAVAKQL 129
           SS +  LL       +EG+PPASKASI+A+P V +  +    ECVICL+EW+     K++
Sbjct: 72  SSGMNPLLRSLLESREEGRPPASKASIDAMPIVEI--DGCEGECVICLEEWKSEETVKEM 131

Query: 130 PCKHKFHPNCIEKWLGIHGNCPVCRYQLPVDGKKNDVGLRARSVREIWVGFSLNNDRRSD 185
           PCKH+FH  CIEKWLG HG+CPVCRY++PVDG  +++G +     EIWV FS N+ RR  
Sbjct: 132 PCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDG--DEIGKKRNDGNEIWVRFSFNDGRRIR 188

[Close]

BLAST of Spg011064 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 3.9e-22
Identity = 70/200 (35.00%), Postives = 99/200 (49.50%), Query Frame = 0


Query: 2   ASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNRDPNPQNENRSERFFLVNPFTQSMV 61
           AS +PN+P +S    T  G  D   F PF F  ++ +    +  + E     NP  Q   
Sbjct: 108 ASIDPNNPSLSG--PTRSGRGDPFAFDPFTFIQNHLNDLRSSGAQIEFVIQNNPSDQGFR 167

Query: 62  LIEG------SSSLEALLLDFG----GKEGQPPASKASIEALPTVRLMEEELPCE---CV 121
           L            LE L+         + G PPASK++IEALP V + +  L  E   C 
Sbjct: 168 LPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCA 227

Query: 122 ICLDEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVDGKKNDVGLRARSVR 181
           +C+D++E G  AKQ+PCKH +H +C+  WL +H +CPVCR++LP D    D   R R  +
Sbjct: 228 VCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDP--DYERRVRGAQ 287

Query: 182 EIWVGFSLNND-RRSDDSNR 188
               G   +N  +RSD  NR
Sbjct: 288 GTSGGNDGDNSGQRSDGDNR 303

[Close]

BLAST of Spg011064 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.8e-20
Identity = 40/77 (51.95%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 81  GQPPASKASIEALPTVRLMEEELPCE---CVICLDEWEIGAVAKQLPCKHKFHPNCIEKW 140
           G PPASK++I+ALPTV++ ++ L  E   C +C+DE+E G+  KQ+PCKH FH +C+  W
Sbjct: 187 GTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPW 246

Query: 141 LGIHGNCPVCRYQLPVD 155
           L +H +CPVCR++LP D
Sbjct: 247 LELHNSCPVCRFELPTD 263

[Close]

BLAST of Spg011064 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.8e-20
Identity = 40/80 (50.00%), Postives = 58/80 (72.50%), Query Frame = 0


Query: 79  KEGQPPASKASIEALPTVRLMEEELPCECVICLDEWEIGAVAKQLPCKHKFHPNCIEKWL 138
           + G PPA K ++EALPTV++ E      C +CLD+ E+G+ AKQ+PC+HKFH +CI  WL
Sbjct: 174 RNGTPPAKKEAVEALPTVKIEE---VVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWL 233

Query: 139 GIHGNCPVCRYQLPVDGKKN 159
            +H +CPVCR++LP +  K+
Sbjct: 234 ELHSSCPVCRFELPSEETKD 250

[Close]

BLAST of Spg011064 vs. ExPASy Swiss-Prot
Match: Q8RXD3 (E3 ubiquitin-protein ligase AIP2 OS=Arabidopsis thaliana OX=3702 GN=AIP2 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 9.0e-19
Identity = 44/101 (43.56%), Postives = 58/101 (57.43%), Query Frame = 0


Query: 62  LIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEEL-----PCECVICLDEWEI 121
           LI G   +   +LD GG    PPASK  +E LP +   EE L       EC IC +   I
Sbjct: 180 LINGLDMIIPDILDDGGPPRAPPASKEVVEKLPVIIFTEELLKKFGAEAECCICKENLVI 239

Query: 122 GAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVDGKK 158
           G   ++LPCKH FHP C++ WL  H +CP+CR++LP D +K
Sbjct: 240 GDKMQELPCKHTFHPPCLKPWLDEHNSCPICRHELPTDDQK 280

[Close]

BLAST of Spg011064 vs. ExPASy TrEMBL
Match: A0A6J1K8F1 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111492631 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.1e-75
Identity = 148/202 (73.27%), Postives = 166/202 (82.18%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNRDP-----NP-----QNENRSERF 60
           MAS+  NSPEISSV +T +GNRDLSLFMPFLFGFS  DP     NP     QN NRS+R 
Sbjct: 38  MASDS-NSPEISSVFDTFIGNRDLSLFMPFLFGFSTADPLQQSQNPADPDRQNANRSDRI 97

Query: 61  FLVNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVIC 120
           FLVNPFTQSMV+IEG SSLEAL+ D GGK+GQPPAS+ASIEALPTV++M EEL CECVIC
Sbjct: 98  FLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEELGCECVIC 157

Query: 121 LDEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVD-GKKNDVGLRARSVRE 180
           LDEWEIGAVAK+LPC HKFHP+CIEKWLG+HGNCP+CR +LP D  +KN+ G RA   RE
Sbjct: 158 LDEWEIGAVAKELPCGHKFHPDCIEKWLGVHGNCPLCRCKLPDDVDRKNNEGRRA---RE 217

Query: 181 IWVGFSLNNDRRSDDSNRVSSD 192
           IWVGFS NNDRRS+ SN VSSD
Sbjct: 218 IWVGFSFNNDRRSEGSNGVSSD 235

[Close]

BLAST of Spg011064 vs. ExPASy TrEMBL
Match: A0A6J1GCB1 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC111452865 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 4.6e-74
Identity = 147/202 (72.77%), Postives = 165/202 (81.68%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNRDP-----NP-----QNENRSERF 60
           MAS+  NSPEISSV +TL+GNRDLSLFMP LFGFS  DP     NP     QN NRS+R 
Sbjct: 1   MASDS-NSPEISSVFDTLIGNRDLSLFMPVLFGFSAADPLQQSQNPADPDRQNANRSDRI 60

Query: 61  FLVNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVIC 120
           FLVNPFTQSMV+IEG SSLEAL+ D GGK+GQPPAS+ASIEALPTV++M EE  CECVIC
Sbjct: 61  FLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAEESGCECVIC 120

Query: 121 LDEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVD-GKKNDVGLRARSVRE 180
           LDEW+IGAVAK+LPC HKFHP+CIEKWLGIHGNCP+CR +LP D  +KN+ G RA   RE
Sbjct: 121 LDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNEGRRA---RE 180

Query: 181 IWVGFSLNNDRRSDDSNRVSSD 192
           IWVGFS NNDRRS+ SN VSSD
Sbjct: 181 IWVGFSFNNDRRSEGSNGVSSD 198

[Close]

BLAST of Spg011064 vs. ExPASy TrEMBL
Match: A0A6J1DR69 (E3 ubiquitin-protein ligase RNF181 OS=Momordica charantia OX=3673 GN=LOC111023495 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.6e-66
Identity = 135/200 (67.50%), Postives = 152/200 (76.00%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSNR---DPNPQNEN----RSERFFLV 60
           MAS EP SPE SS          L  F PFLFGF+N    DPN ++ N     SERFFLV
Sbjct: 1   MASSEPESPETSS----------LFAFTPFLFGFTNNRRDDPNQESRNPANRESERFFLV 60

Query: 61  NPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVICLDE 120
           NPFTQSMV+IEGSS+LE+LL D G K+G PPASKASIEALP V+L+EE    ECVICLDE
Sbjct: 61  NPFTQSMVVIEGSSALESLLRDLGSKDGPPPASKASIEALPAVQLLEEG---ECVICLDE 120

Query: 121 WEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPV---DGKKNDVGLRARSVREI 180
           WEIGA AK+LPCKHKFHPNCIEKWLGIHG+CP+CR+++PV   DGKKNDV  R R +REI
Sbjct: 121 WEIGATAKELPCKHKFHPNCIEKWLGIHGSCPICRHKMPVDDGDGKKNDVVGRGRRLREI 180

Query: 181 WVGFSLNNDRRSDDSNRVSS 191
           WVGFS NND RSDDSN + S
Sbjct: 181 WVGFSFNNDIRSDDSNPLPS 187

[Close]

BLAST of Spg011064 vs. ExPASy TrEMBL
Match: A0A6P4B064 (E3 ubiquitin-protein ligase MPSR1-like OS=Ziziphus jujuba OX=326968 GN=LOC107429014 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 4.9e-60
Identity = 118/202 (58.42%), Postives = 154/202 (76.24%), Query Frame = 0


Query: 3   SEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSN-------RDPNPQNENRS---ERFFL 62
           + E ++ E+S++ E LV NRDLSLF+PF+FGF+N       +DP+ +NENR+   +R  L
Sbjct: 2   ASEADASELSTMFERLVRNRDLSLFLPFIFGFANSLPRANPQDPDEENENRATSRDRVIL 61

Query: 63  VNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVICLD 122
           +NPFTQ MV+I+G SSL+++L D GGK+GQPPASKASIEA+P V   EE L  ECVICL+
Sbjct: 62  INPFTQGMVVIDGFSSLDSVLRDLGGKDGQPPASKASIEAMPGVETEEEGL--ECVICLE 121

Query: 123 EWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVD----GKKNDVGLRARSVR 182
           E+E+G VAK++PCKHKFH +CIEKWLGIHG+CPVCR+ +PVD    GKK +   R R   
Sbjct: 122 EFEVGGVAKEMPCKHKFHGDCIEKWLGIHGSCPVCRHNMPVDEEEMGKKREEEGRERRRG 181

Query: 183 EIWVGFSLNNDRRSDDSNRVSS 191
           E+WV FS N+ RRS+DSN+VSS
Sbjct: 182 EVWVSFSFNSGRRSEDSNQVSS 201

[Close]

BLAST of Spg011064 vs. ExPASy TrEMBL
Match: A0A4U5MX71 (Zinc finger family protein OS=Populus alba OX=43335 GN=D5086_0000294610 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.7e-57
Identity = 114/202 (56.44%), Postives = 143/202 (70.79%), Query Frame = 0


Query: 1   MASEEPNSPEISSVLETLVGNRDLSLFMPFLFGFSN------RDPN---PQNENRSERFF 60
           MASE     E+SS+ E L+ +RDLSLF+PF+ GF++      RDP+   PQ  + +ER  
Sbjct: 1   MASENEFPAELSSMFERLLRHRDLSLFLPFILGFTSTNTTEQRDPDQEAPQTTDTNERII 60

Query: 61  LVNPFTQSMVLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVICL 120
           L+NPFTQ MV+IEG++SLE+LL D G K GQPPASKASIEA+P V + E+    EC ICL
Sbjct: 61  LINPFTQGMVVIEGAASLESLLRDIGNKNGQPPASKASIEAMPKVEIGEDNKDGECAICL 120

Query: 121 DEWEIGAVAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVD----GKK---NDVGLRA 180
           +EWE+G V K++PCKH+FH  C+EKWL IHGNCPVCRY++PVD    GKK    DVG   
Sbjct: 121 EEWELGGVVKEMPCKHRFHGGCVEKWLKIHGNCPVCRYKMPVDEEELGKKRDEGDVGRER 180

Query: 181 RSVREIWVGFSLNNDRRSDDSN 187
           R  REIWV F+ N  RR+ DSN
Sbjct: 181 RVEREIWVSFAFNGSRRNGDSN 202

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BLAST of Spg011064 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.0e-46
Identity = 90/181 (49.72%), Postives = 122/181 (67.40%), Query Frame = 0


Query: 10  EISSVLETLVGNRDLSLFMPFLFGFSNRDPNPQNE----NRSERFFLVNPFTQSMVLIEG 69
           E SSV    + NRDL LF+PFL GFS+++ +  ++    +  ER  LVNPFTQ M+++EG
Sbjct: 12  ETSSVSGRFLRNRDLYLFLPFLLGFSDQESSNGDDDDVASSRERIILVNPFTQGMIVLEG 71

Query: 70  SSSLEALLLDF--GGKEGQPPASKASIEALPTVRLMEEELPCECVICLDEWEIGAVAKQL 129
           SS +  LL       +EG+PPASKASI+A+P V +  +    ECVICL+EW+     K++
Sbjct: 72  SSGMNPLLRSLLESREEGRPPASKASIDAMPIVEI--DGCEGECVICLEEWKSEETVKEM 131

Query: 130 PCKHKFHPNCIEKWLGIHGNCPVCRYQLPVDGKKNDVGLRARSVREIWVGFSLNNDRRSD 185
           PCKH+FH  CIEKWLG HG+CPVCRY++PVDG  +++G +     EIWV FS N+ RR  
Sbjct: 132 PCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDG--DEIGKKRNDGNEIWVRFSFNDGRRIR 188

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BLAST of Spg011064 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 4.7e-31
Identity = 69/185 (37.30%), Postives = 105/185 (56.76%), Query Frame = 0


Query: 3   SEEPNSPEISSVLETLVGNR--DLSLFMPFLFGFSNRDPNPQNENRSERFFLVNPFTQSM 62
           S E +  E SS+ E ++  R   LS F+P +   + R+ +    +  +     +P +   
Sbjct: 2   SSENDFVEFSSMFERIIQGRGDGLSRFLPVIVALAAREDDDDQGSTDQTTRRGDPLSPRF 61

Query: 63  VLIEGSSSLEALLLDFGGKEGQPPASKASIEALPTVRLME--EELPCECVICLDEWEIGA 122
           V+I   S L+    D GGK+G+ PA K+ +E +P V + E  E+    C ICLDEW  G 
Sbjct: 62  VMIGSRSGLDDFFSD-GGKQGRSPALKSEVENMPRVVIGEDKEKYGGSCAICLDEWSKGD 121

Query: 123 VAKQLPCKHKFHPNCIEKWLGIHGNCPVCRYQLPVDGKKNDVGLRARSVREIWVGFSLN- 182
           VA ++PCKHKFH  C+E+WLG H  CP+CRY++PV+  + +  +       IW+GFS+N 
Sbjct: 122 VAAEMPCKHKFHSKCVEEWLGRHATCPMCRYEMPVEEVEEEKKI------GIWIGFSINA 179

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BLAST of Spg011064 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 119.8 bits (299), Expect = 2.4e-27
Identity = 65/185 (35.14%), Postives = 94/185 (50.81%), Query Frame = 0


Query: 34  FSNRDPNPQNENRSERFFLVNPFTQSMVLIEGSSSLEALLLDFG---------------- 93
           + + D N  N    +R  ++NP+ QS+V+    +     L   G                
Sbjct: 169 YESSDNNWDNSRERDRVIMINPYNQSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLA 228

Query: 94  ----GKEGQPPASKASIEALPTVRLMEEELPCECVICLDEWEIGAVAKQLPCKHKFHPNC 153
                ++G PPA K ++EALPTV++ME   P +C +CLD++E G  AK++PCKHKFH  C
Sbjct: 229 ENDPNRQGTPPARKEAVEALPTVKIME---PLQCSVCLDDFEKGTEAKEMPCKHKFHVRC 288

Query: 154 IEKWLGIHGNCPVCRYQLPVDGKKNDVG-------LRARSVREIWVGFSLNN----DRRS 188
           I  WL +H +CPVCR++LP     +D         LR R+VRE   G  + N    DR  
Sbjct: 289 IVPWLELHSSCPVCRFELPSSADDDDETKTDSERVLRTRNVRETSNGNVVENVGNADRGR 348

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BLAST of Spg011064 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.0e-22
Identity = 46/91 (50.55%), Postives = 62/91 (68.13%), Query Frame = 0


Query: 69  LEALLLDFGGKEGQPPASKASIEALPTVRLMEEELPCECVICLDEWEIGAVAKQLPCKHK 128
           L+ L  +   + G PPA K ++EAL TV++ EE L  +C +CLD++EIG  AK +PC HK
Sbjct: 188 LQRLAENDPNRYGTPPAKKEAVEALATVKI-EETL--QCSVCLDDFEIGTEAKLMPCTHK 247

Query: 129 FHPNCIEKWLGIHGNCPVCRYQLPVDGKKND 160
           FH +C+  WL +H +CPVCRYQLP D  K D
Sbjct: 248 FHSDCLLPWLELHSSCPVCRYQLPADEAKTD 275

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BLAST of Spg011064 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 2.0e-21
Identity = 40/77 (51.95%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 81  GQPPASKASIEALPTVRLMEEELPCE---CVICLDEWEIGAVAKQLPCKHKFHPNCIEKW 140
           G PPASK++I+ALPTV++ ++ L  E   C +C+DE+E G+  KQ+PCKH FH +C+  W
Sbjct: 187 GTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPW 246

Query: 141 LGIHGNCPVCRYQLPVD 155
           L +H +CPVCR++LP D
Sbjct: 247 LELHNSCPVCRFELPTD 263

The following BLAST results are available for this feature:

BLAST of Spg011064 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023523370.1	1.3e-75	73.76	E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997776.1	2.2e-75	73.27	E3 ubiquitin-protein ligase RNF181-like [Cucurbita maxima]	[more]
XP_022949551.1	9.4e-74	72.77	E3 ubiquitin-protein ligase RNF181-like [Cucurbita moschata]	[more]
KAG6607122.1	9.4e-74	72.77	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022156648.1	3.2e-66	67.50	E3 ubiquitin-protein ligase RNF181 [Momordica charantia]	[more]

BLAST of Spg011064 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	1.5e-45	49.72	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
P0CH30	3.9e-22	35.00	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q8LPN7	2.8e-20	51.95	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q6AVN2	2.8e-20	50.00	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8RXD3	9.0e-19	43.56	E3 ubiquitin-protein ligase AIP2 OS=Arabidopsis thaliana OX=3702 GN=AIP2 PE=1 SV...	[more]

BLAST of Spg011064 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K8F1	1.1e-75	73.27	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]
A0A6J1GCB1	4.6e-74	72.77	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1DR69	1.6e-66	67.50	E3 ubiquitin-protein ligase RNF181 OS=Momordica charantia OX=3673 GN=LOC11102349...	[more]
A0A6P4B064	4.9e-60	58.42	E3 ubiquitin-protein ligase MPSR1-like OS=Ziziphus jujuba OX=326968 GN=LOC107429...	[more]
A0A4U5MX71	1.7e-57	56.44	Zinc finger family protein OS=Populus alba OX=43335 GN=D5086_0000294610 PE=4 SV=...	[more]

BLAST of Spg011064 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	1.0e-46	49.72	RING/U-box superfamily protein	[more]
AT1G14200.1	4.7e-31	37.30	RING/U-box superfamily protein	[more]
AT5G56340.1	2.4e-27	35.14	RING/U-box superfamily protein	[more]
AT1G55530.1	4.0e-22	50.55	RING/U-box superfamily protein	[more]
AT3G19950.1	2.0e-21	51.95	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 107..147 e-value: 1.8E-7 score: 40.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 106..148 e-value: 3.3E-12 score: 46.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 107..148 score: 12.179465
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 76..154 e-value: 4.1E-21 score: 76.5
None	No IPR available	PANTHER	PTHR15710:SF96	SUBFAMILY NOT NAMED	coord: 10..157
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 10..157
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 106..148 e-value: 4.55221E-19 score: 74.6457
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 90..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg011064.1	Spg011064.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity