Spg010932 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg010932 vs. NCBI nr
Match: XP_022150071.1 (senescence-associated carboxylesterase 101 [Momordica charantia])

HSP 1 Score: 844.0 bits (2179), Expect = 7.9e-241
Identity = 427/584 (73.12%), Postives = 487/584 (83.39%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           MDD+NS RFSSGLELAS IATSDLLEHSW+AITK RS+I+AN      F   YNERSNC+
Sbjct: 1   MDDENSLRFSSGLELASFIATSDLLEHSWRAITKQRSKISANGSRFSAFFGRYNERSNCE 60

Query: 61  VIAFVTTPNFTENPLE-KGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLFSPF 120
           +IAFVT PN  ENPLE + GGDLV  S   D F AFEFLCSK CSD AINGAAL+LF  F
Sbjct: 61  IIAFVTAPNCRENPLEGERGGDLVSFSDFKDVFPAFEFLCSKQCSDFAINGAALELFWDF 120

Query: 121 YHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPLL 180
           Y +YEQEI+RL SNP TQLIITG ALGG VASLFTL LLD LDLTK KRPLCIT+GSPLL
Sbjct: 121 YDVYEQEIVRLKSNPKTQLIITGDALGGSVASLFTLFLLDFLDLTKAKRPLCITFGSPLL 180

Query: 181 GNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSEDP 240
           G+E LQ AIS+FSTWSSCFLHVVSNHDPLPR FLNH  YYPFGTFLFCS+S GCACS DP
Sbjct: 181 GDEALQSAISQFSTWSSCFLHVVSNHDPLPRMFLNHTAYYPFGTFLFCSESDGCACSGDP 240

Query: 241 TSILKLLEATAVQN--NPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQ 300
            ++ KLLEATAV N  +   SLLFDYKET+D L N+ NLK ++ LITENTESLRGSF+AQ
Sbjct: 241 ATVSKLLEATAVPNVTSNSHSLLFDYKETVDHLINQANLKAMSMLITENTESLRGSFVAQ 300

Query: 301 LDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 360
           L+AIGVV+ QAQ+ QN+DVN+L   +I  E D I + TK SDPS+ LN +KI MA LEWY
Sbjct: 301 LEAIGVVQIQAQQQQNVDVNKLVKKMIERENDTILE-TKTSDPSEALNMVKINMAKLEWY 360

Query: 361 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 420
           KKKC+SD+IGYYDSYKN  +  DIRVAEFKK+LRVYWE +VEEA+R+PQREGAPFN RW+
Sbjct: 361 KKKCQSDDIGYYDSYKNQRHPPDIRVAEFKKKLRVYWEKMVEEAERRPQREGAPFNIRWM 420

Query: 421 GGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEESTSNSSTTQRESVSSIL 480
            GGTNYRRMVEPLDIAEHYRKGL +Y+SHRPKHY++LEEW EES+S+ ST Q+ESVSSIL
Sbjct: 421 FGGTNYRRMVEPLDIAEHYRKGLRDYKSHRPKHYEKLEEWFEESSSDPSTGQKESVSSIL 480

Query: 481 TVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSEIFLP 540
           TVDSCFWA+VEEAILACQV+K+V+CSA + E A+ KL  FEDYVV +M+N AVSSEIFLP
Sbjct: 481 TVDSCFWANVEEAILACQVVKMVDCSAEEEEEAMGKLKDFEDYVVRMMKNSAVSSEIFLP 540

Query: 541 NSSFMTWWKEYDTIMGDDHDSVLTRLMRNE-QYEEYANGRLEIP 581
            SSFM WW++YD I+GDDHDS+L+ LMRNE  YE+YANGRLEIP
Sbjct: 541 QSSFMRWWEQYDEIVGDDHDSILSVLMRNEDHYEQYANGRLEIP 583

BLAST of Spg010932 vs. NCBI nr
Match: XP_038906094.1 (senescence-associated carboxylesterase 101 [Benincasa hispida])

HSP 1 Score: 829.7 bits (2142), Expect = 1.5e-236
Identity = 424/587 (72.23%), Postives = 486/587 (82.79%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           MDD NSHRFSSGLEL + IATSDLLEHSWKAITK+R+EIT   P+ L F++YYNERSNCK
Sbjct: 1   MDDHNSHRFSSGLELGNFIATSDLLEHSWKAITKLRTEITV--PIYLAFYKYYNERSNCK 60

Query: 61  VIAFVTTPNFTENPL-EKGGGDLVPLSG-LNDAFTAFEFLCSKGCSDSAINGAALDLFSP 120
           ++AFVTTPN TENP+ E GG DLV LS  L D FTAFEFL +K C DSAING ALDLF  
Sbjct: 61  ILAFVTTPNCTENPIDEDGGEDLVSLSDLLIDEFTAFEFLQTKTCPDSAINGVALDLFRR 120

Query: 121 FYHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPL 180
           FY +YEQEI RLIS+PNT +IITGH LGG VASL TLLLLD +DLTKTKRPLCIT+GSPL
Sbjct: 121 FYPVYEQEISRLISDPNTPIIITGHGLGGSVASLLTLLLLDSIDLTKTKRPLCITFGSPL 180

Query: 181 LGNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSED 240
           LGNE  Q AIS+FSTWSSCFLH+VSN DPLP +FLN++ YYPFGTFLFCS SGGC+  ED
Sbjct: 181 LGNEAFQNAISQFSTWSSCFLHLVSNQDPLPTKFLNNKAYYPFGTFLFCSQSGGCSSFED 240

Query: 241 PTSILKLLEATAVQNNPL-GSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQ 300
           P S+LKLLEAT   N+ L  S  FDYKETIDRL N+ NLK   +++TEN ES  GSF+AQ
Sbjct: 241 PKSVLKLLEATKASNDVLTASSFFDYKETIDRLINQANLKATMKMVTENAESWTGSFVAQ 300

Query: 301 LDAIGVVKNQAQRYQN---LDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYL 360
           L+ IGV +NQAQ+ Q    +DVNRLA  L + EM++I + TK    +KTLND+KI MA L
Sbjct: 301 LETIGVARNQAQQQQQQRVVDVNRLAKALKDNEMNLILENTKPF--AKTLNDVKINMAKL 360

Query: 361 EWYKKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNT 420
           EWYKKKCK DNIGYYDSYKN  +E DIRVAEFKK LRVYWEN VEEA+RKP R+GAPFN 
Sbjct: 361 EWYKKKCKFDNIGYYDSYKNQRSEPDIRVAEFKKILRVYWENKVEEAERKPLRDGAPFNV 420

Query: 421 RWLGGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEES-TSNSSTTQRESV 480
           + L GGTNYRRMVEPLDIAEHYRKGL +Y+SHRPKHY +LE+W +ES T +SS+TQRE+V
Sbjct: 421 QLLFGGTNYRRMVEPLDIAEHYRKGLTDYKSHRPKHYTKLEQWFKESPTPDSSSTQREAV 480

Query: 481 SSILTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSE 540
           SSILTVDS FW HVEEA+LACQVL +V+CS  + EV +AKL  FEDYVVELMRNYAVSSE
Sbjct: 481 SSILTVDSLFWEHVEEAVLACQVLTMVDCSEEENEVELAKLREFEDYVVELMRNYAVSSE 540

Query: 541 IFLPNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGRLEIP 581
           IFLP+S+FM WW+EYD I+GDDHDSVL+RLMRNE+YEEYANGRL+IP
Sbjct: 541 IFLPHSTFMKWWEEYDEIVGDDHDSVLSRLMRNEEYEEYANGRLQIP 583

BLAST of Spg010932 vs. NCBI nr
Match: XP_022983733.1 (senescence-associated carboxylesterase 101 [Cucurbita maxima])

HSP 1 Score: 821.2 bits (2120), Expect = 5.5e-234
Identity = 416/584 (71.23%), Postives = 480/584 (82.19%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           M D+N HRFSSGLE+A+ IATSDLL+HSWKAITK+R+ ITA++  CL F  +YNERSNCK
Sbjct: 1   MADNNPHRFSSGLEIANLIATSDLLDHSWKAITKLRTVITADESKCLAFFGHYNERSNCK 60

Query: 61  VIAFVTTPNFTENPL-EKGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLFSPF 120
           ++AFVTTPN +ENPL E+GG DLV LS L D FTAFEFL S+ CSDSAINGAALDLF PF
Sbjct: 61  ILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCSDSAINGAALDLFGPF 120

Query: 121 YHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPLL 180
           Y +YEQEI RL SNP TQLIITGH LGG VASLFTLLLLD +DLTK KRPLCIT+GSPLL
Sbjct: 121 YPIYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLTKAKRPLCITFGSPLL 180

Query: 181 GNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSEDP 240
           GNE LQ AIS+FSTWSSCFLHVVSN DPLP   LN++ YYPFGTFLFCS+SGGC+C EDP
Sbjct: 181 GNEALQSAISQFSTWSSCFLHVVSNRDPLPTSLLNNKAYYPFGTFLFCSESGGCSCFEDP 240

Query: 241 TSILKLLEATAVQNNPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQLD 300
            S LKLLEAT    +   S  FDYKETI RL ++ NLK  T LITEN+ S   SF+AQL+
Sbjct: 241 KSTLKLLEATKANTHLTASSFFDYKETIHRLIDQANLKATTRLITENSVSRTASFIAQLE 300

Query: 301 AIGVVKNQAQRYQN--LDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 360
           AIGVV +QAQ+ Q   +DV RLA  L + EM+MIF+KT +S  +KTLND+KI MA LEWY
Sbjct: 301 AIGVVPDQAQQQQQRVVDVKRLAVELKDNEMNMIFEKTNSS--AKTLNDVKINMAKLEWY 360

Query: 361 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 420
           KKKC S++IGYYDSYKN  +E DI+VAEFKK L VYWEN VEEA+RKP +EG+P N R L
Sbjct: 361 KKKCNSESIGYYDSYKNGRSEPDIKVAEFKKILLVYWENKVEEAERKPVKEGSPLNVRLL 420

Query: 421 GGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEESTS-NSSTTQRESVSSI 480
            GGTNYRRMVEPLDIAEHYRKGL NY++HRPKHY++LEEW  EST+  SS+TQRESVSSI
Sbjct: 421 FGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNESTTPESSSTQRESVSSI 480

Query: 481 LTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSEIFL 540
           LTVDS FW HVEEA++ACQVL+ V+ SA ++E  +AKL  FE+YVVELM++Y VSSEIFL
Sbjct: 481 LTVDSLFWEHVEEAVVACQVLRTVDSSAEEKEAELAKLKEFEEYVVELMKSYGVSSEIFL 540

Query: 541 PNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGRLEIP 581
           P SSFM WW EYD I+GD HDSVLT LMRNE+Y+EYANGRL+IP
Sbjct: 541 PQSSFMRWWDEYDGIVGDGHDSVLTMLMRNEEYKEYANGRLDIP 582

BLAST of Spg010932 vs. NCBI nr
Match: XP_023526959.1 (senescence-associated carboxylesterase 101 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 817.0 bits (2109), Expect = 1.0e-232
Identity = 413/583 (70.84%), Postives = 475/583 (81.48%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           M D+N  RFSSGLE+A+ IATSDLL+HSWKAITK+R+ ITA++  CL F  +YNERSNCK
Sbjct: 1   MADNNPQRFSSGLEIANLIATSDLLDHSWKAITKLRTVITADESTCLAFFGHYNERSNCK 60

Query: 61  VIAFVTTPNFTENPLEKGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLFSPFY 120
           ++AFVTTPN +ENPLE+GG DLV LS L D FTAFEFL S+ C DSAING ALDLF  FY
Sbjct: 61  ILAFVTTPNCSENPLEEGGEDLVSLSDLKDEFTAFEFLYSESCPDSAINGVALDLFGRFY 120

Query: 121 HLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPLLG 180
            +YEQEI RL SNP TQLIITGH LGG VASLFTLLLLD +DLTK KRPLCIT+GSPLLG
Sbjct: 121 PVYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLTKAKRPLCITFGSPLLG 180

Query: 181 NEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSEDPT 240
           N+ LQ AIS+FSTWSSCFLHVVSNHDPLP   LN++ YYPFGTFLFCS+SGGC+C EDP 
Sbjct: 181 NKALQSAISQFSTWSSCFLHVVSNHDPLPTSLLNNKAYYPFGTFLFCSESGGCSCFEDPK 240

Query: 241 SILKLLEATAVQNNPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQLDA 300
           S  KLLEAT    +      FDYKETI RL ++ NLK  T LITEN+ES   SF+AQL+A
Sbjct: 241 STSKLLEATKANTHLTAFSFFDYKETILRLIDQANLKATTRLITENSESWTASFIAQLEA 300

Query: 301 IGVVKNQAQRYQN--LDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWYK 360
           IGVV +QAQ+ Q   +DV RLA  L   EM+MIF+KT     +KTLND+KI MA LEWYK
Sbjct: 301 IGVVPDQAQQQQQRAVDVKRLAMALQGNEMNMIFEKTNPF--AKTLNDVKINMAKLEWYK 360

Query: 361 KKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWLG 420
           KKC S++IGYYDSYKN   E DI+VAEFKK L VYWEN VEEA+RKP +EG+P N R L 
Sbjct: 361 KKCNSESIGYYDSYKNGRTEPDIKVAEFKKILLVYWENKVEEAERKPVKEGSPLNVRLLF 420

Query: 421 GGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEES-TSNSSTTQRESVSSIL 480
           GGTNYRRMVEPLDIAEHYRKGL NY++HRPKHY++LEEW  ES T  SS+TQRESVSSIL
Sbjct: 421 GGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNESMTPESSSTQRESVSSIL 480

Query: 481 TVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSEIFLP 540
           TVDS FWAHVEEA++ACQVL++V+ SA ++E  +AKL  FE+YVVELM+NY VSSEIFLP
Sbjct: 481 TVDSLFWAHVEEAVVACQVLRMVDSSAEEKEAELAKLKEFEEYVVELMKNYGVSSEIFLP 540

Query: 541 NSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGRLEIP 581
           +SSFM WW EYD I+GDDHDSVLT LMRNE+Y+EYANGRL+IP
Sbjct: 541 HSSFMRWWDEYDGIVGDDHDSVLTMLMRNEEYKEYANGRLDIP 581

BLAST of Spg010932 vs. NCBI nr
Match: XP_023526958.1 (senescence-associated carboxylesterase 101 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 813.9 bits (2101), Expect = 8.8e-232
Identity = 414/584 (70.89%), Postives = 476/584 (81.51%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           M D+N  RFSSGLE+A+ IATSDLL+HSWKAITK+R+ ITA++  CL F  +YNERSNCK
Sbjct: 1   MADNNPQRFSSGLEIANLIATSDLLDHSWKAITKLRTVITADESTCLAFFGHYNERSNCK 60

Query: 61  VIAFVTTPNFTENPL-EKGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLFSPF 120
           ++AFVTTPN +ENPL E+GG DLV LS L D FTAFEFL S+ C DSAINGAALDLF  F
Sbjct: 61  ILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCPDSAINGAALDLFGRF 120

Query: 121 YHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPLL 180
           Y +YEQEI RL SNP TQLIITGH LGG VASLFTLLLLD +DLTK KRPLCIT+GSPLL
Sbjct: 121 YPVYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLTKAKRPLCITFGSPLL 180

Query: 181 GNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSEDP 240
           GN+ LQ AIS+FSTWSSCFLHVVSNHDPLP   LN++ YYPFGTFLFCS+SGGC+C EDP
Sbjct: 181 GNKALQSAISQFSTWSSCFLHVVSNHDPLPTSLLNNKAYYPFGTFLFCSESGGCSCFEDP 240

Query: 241 TSILKLLEATAVQNNPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQLD 300
            S  KLLEAT    +      FDYKETI RL ++ NLK  T LITEN+ES   SF+AQL+
Sbjct: 241 KSTSKLLEATKANTHLTAFSFFDYKETILRLIDQANLKATTRLITENSESWTASFIAQLE 300

Query: 301 AIGVVKNQAQRYQN--LDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 360
           AIGVV +QAQ+ Q   +DV RLA  L   EM+MIF+KT     +KTLND+KI MA LEWY
Sbjct: 301 AIGVVPDQAQQQQQRAVDVKRLAMALQGNEMNMIFEKTNPF--AKTLNDVKINMAKLEWY 360

Query: 361 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 420
           KKKC S++IGYYDSYKN   E DI+VAEFKK L VYWEN VEEA+RKP +EG+P N R L
Sbjct: 361 KKKCNSESIGYYDSYKNGRTEPDIKVAEFKKILLVYWENKVEEAERKPVKEGSPLNVRLL 420

Query: 421 GGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEES-TSNSSTTQRESVSSI 480
            GGTNYRRMVEPLDIAEHYRKGL NY++HRPKHY++LEEW  ES T  SS+TQRESVSSI
Sbjct: 421 FGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNESMTPESSSTQRESVSSI 480

Query: 481 LTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSEIFL 540
           LTVDS FWAHVEEA++ACQVL++V+ SA ++E  +AKL  FE+YVVELM+NY VSSEIFL
Sbjct: 481 LTVDSLFWAHVEEAVVACQVLRMVDSSAEEKEAELAKLKEFEEYVVELMKNYGVSSEIFL 540

Query: 541 PNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGRLEIP 581
           P+SSFM WW EYD I+GDDHDSVLT LMRNE+Y+EYANGRL+IP
Sbjct: 541 PHSSFMRWWDEYDGIVGDDHDSVLTMLMRNEEYKEYANGRLDIP 582

BLAST of Spg010932 vs. ExPASy Swiss-Prot
Match: Q4F883 (Senescence-associated carboxylesterase 101 OS=Arabidopsis thaliana OX=3702 GN=SAG101 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.6e-81
Identity = 214/589 (36.33%), Postives = 295/589 (50.08%), Query Frame = 0

Query: 12  GLELASSIATSDLLEHSWKAITKVRSEITAN-----------KPMCLVFHRYYNERSNCK 71
           G  L   + TS LL  SW  I ++ +   +N           K   L F  +  E+    
Sbjct: 9   GSALGKLVVTSGLLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLV 68

Query: 72  VIAFVTTPNFTENPLEKGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLF---S 131
           V        F+  P+ +       L  + D    F FLCS+     +++  A +LF   S
Sbjct: 69  V--------FSAPPICRSSSSDSTLLHVKDKENPFPFLCSENNPSFSLHTPAFNLFTSAS 128

Query: 132 PFYHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSP 191
                 + E+L+ + +    +IITG ALGG VASL+TL LL+ ++ T  KRPLCIT+GSP
Sbjct: 129 TSLTYLKSELLQTLKS-EKPVIITGAALGGSVASLYTLWLLETIEPT-LKRPLCITFGSP 188

Query: 192 LLGNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSE 251
           L+G+  LQ+ +   S  +SCFLHVVS    +   F     + PFGTFL C DS GC C E
Sbjct: 189 LIGDASLQQILEN-SVRNSCFLHVVSAQTRIKMDF-----FKPFGTFLICFDS-GCVCIE 248

Query: 252 DPTSILKLLEATAVQNNPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQ 311
           D  ++ +LL      N    S L DY + ++RL              ++  SL  S +  
Sbjct: 249 DHVAVTELL------NGVHDSGLVDYSQVLNRL-------------DQSMLSLADSRLIP 308

Query: 312 LDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 371
            D I  ++ +A+             + N   DM+F         K LND+KI MAY+EWY
Sbjct: 309 EDVIKGIEKRAE-------------MKNLRFDMMF---------KKLNDMKISMAYIEWY 368

Query: 372 KKKCKSDNIGYYDSYKN----ASNEFDIRVA-EFKKRLRVYWENIVEEAKRKPQREGAPF 431
           KKKCK   IGYYD +K      S EFDI +    K  L  +W+++VEE +R+PQ + +  
Sbjct: 369 KKKCKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASIL 428

Query: 432 NTRWLGGGTNYRRMVEPLDIAEHYRKGLNNYE-SHRPKHYKQLEEW--LEESTSNSSTTQ 491
             R+L  G NYRRM+EPLDIAE+Y +G   Y  + R  HY  LE+W  +E         +
Sbjct: 429 KRRFLFSGNNYRRMIEPLDIAEYYLEGRKEYRTTGRSHHYVMLEKWFGMESILIEKERCK 488

Query: 492 RESVSSILTVDSCFWAHVEEAILACQVLKIVNCSARD--REVAVAKLTTFEDYVVELMRN 551
           +  +S +LT DSCFWA VE++++    L       RD  REV   KL  FE YV E++  
Sbjct: 489 KRDLSDLLTFDSCFWAEVEDSLIVINQLN-TTVGMRDDVREVLTRKLVEFEGYVWEIITK 537

Query: 552 YAVSSEIFLPNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGR 577
             VS EIFL  SSFM WWKEY  I G  + S LT  M   +YE Y   +
Sbjct: 549 REVSPEIFLEESSFMKWWKEYKKIKG-FNSSYLTEFMNTRKYESYGKSQ 537

BLAST of Spg010932 vs. ExPASy Swiss-Prot
Match: Q9S745 (Lipase-like PAD4 OS=Arabidopsis thaliana OX=3702 GN=PAD4 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 5.3e-38
Identity = 127/454 (27.97%), Postives = 213/454 (46.92%), Query Frame = 0

Query: 119 FYHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPL 178
           F  L  +E L L       ++ITGH+ GG +A+ FT L L       + R  CIT+GSPL
Sbjct: 93  FLQLKIKEGLELELLGKKLVVITGHSTGGALAA-FTALWLLSQSSPPSFRVFCITFGSPL 152

Query: 179 LGNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSED 238
           LGN+ L  +IS+ S  +  F HVVS HD +PR   ++  ++PFGT+LFCSD GG  C ++
Sbjct: 153 LGNQSLSTSISR-SRLAHNFCHVVSIHDLVPRS--SNEQFWPFGTYLFCSDKGG-VCLDN 212

Query: 239 PTSI---LKLLEATAVQNNPLGSLLFDYKETIDR--LKNRVNLKIVTELITENTESLRGS 298
             S+     +L  TA QN         Y  T+    LK+R     +   I +N  S +  
Sbjct: 213 AGSVRLMFNILNTTATQNTEEHQRYGHYVFTLSHMFLKSR---SFLGGSIPDN--SYQAG 272

Query: 299 FMAQLDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAY 358
               ++A+G   +        +    AT ++   +       ++++ +  L  +      
Sbjct: 273 VALAVEALGFSNDDTSGVLVKECIETATRIVRAPI------LRSAELANELASVLPARLE 332

Query: 359 LEWYKKKC--KSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAP 418
           ++WYK +C    + +GYYD +K  S + D +V   + RL  +W+ +++  +         
Sbjct: 333 IQWYKDRCDASEEQLGYYDFFKRYSLKRDFKVNMSRIRLAKFWDTVIKMVETNELPFDFH 392

Query: 419 FNTRWLGGGTNYRRMVEPLDIAEHYRK-----GLNNYESHRPKHYKQLEEWLEESTSNSS 478
              +W+     Y+ + EPLDIA  Y+      G +  E +RPK Y+ +++W +       
Sbjct: 393 LGKKWIYASQFYQLLAEPLDIANFYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEE 452

Query: 479 TTQRESVSSILTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMR 538
             +    S+  T D+CFWA +E+A       +  +   + R +   K+  FE Y   L+ 
Sbjct: 453 CVRSRYAST--TQDTCFWAKLEQAKEWLDEARKESSDPQRRSLLREKIVPFESYANTLVT 512

Query: 539 NYAVSSEIFLPNSSFMTW---WKEYDTIMGDDHD 558
              VS ++   NSS+  W    KE+   MG +++
Sbjct: 513 KKEVSLDVKAKNSSYSVWEANLKEFKCKMGYENE 528

BLAST of Spg010932 vs. ExPASy Swiss-Prot
Match: Q9SU71 (Protein EDS1B OS=Arabidopsis thaliana OX=3702 GN=EDS1B PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-15
Identity = 107/514 (20.82%), Postives = 189/514 (36.77%), Query Frame = 0

Query: 137 QLIITGHALGGWVASLFTLLLLD---LLDLTKTKRPLCITYGSPLLGNEGLQKAISKFST 196
           Q++ TGH+ GG  A L T+  L+   + D      P C+T+G+PL+G+   + A+ +   
Sbjct: 116 QVVFTGHSFGGATAILATVWYLETYFIRDAYAAPEPRCVTFGAPLVGDYIFKHALGR-EN 175

Query: 197 WSSCFLHVVSNHDPLPRRFLNHR------------------------------------- 256
           WS  F++ V+  D +PR  L  +                                     
Sbjct: 176 WSRFFVNFVTRFDIVPRIMLARKTTIEQTLSYVLGKLDSTRAPIHESDQVITEFYTRVMR 235

Query: 257 -------------------------------GYYPFGTFLFCSDSGGCACSEDPTSILKL 316
                                           Y P GTF+F S         +  +IL++
Sbjct: 236 DTYTVASKAVCQLIGNGEAFLETLSSFYELSPYRPVGTFVF-STQKRLVVVNNSDAILQM 295

Query: 317 LEATAVQNNPLGSLLFDYKETID-----RLKNRVNLKIVTELITENTESLRGSFMAQLDA 376
           L  T   N+     +  +    D      L   + +K++  L       L    +   ++
Sbjct: 296 LFYTCQSNDEQELSVIPFLSIRDHHGYEELVQSIGIKLLNHL------DLHNPLLDGENS 355

Query: 377 IGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLND--IKIMMAYLEWYK 436
           IG          +L ++  A   I+  ++    + +     +T  D  ++ +   +E YK
Sbjct: 356 IG------SALDDLGMSTRARQCIHAALEAEKQRVENQKKIETKRDQIVERLTWIVEVYK 415

Query: 437 KKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWLG 496
            KC++   GYYDS+K+++ E D +    +  L   ++ ++   K+    +G   +  W+ 
Sbjct: 416 PKCQAHKNGYYDSFKDSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDGFEGSRGWIN 475

Query: 497 GGTNYRRMVEPLDIAEHYRKGLNN----YESH-RPKHYKQLEEWLE-------------- 546
             T YRR++EPLDI+ ++ +  N     Y  H RP  YK  +   E              
Sbjct: 476 LATQYRRLIEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILKPTGMIAKDV 535

BLAST of Spg010932 vs. ExPASy Swiss-Prot
Match: Q9XF23 (Protein EDS1L OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 8.2e-15
Identity = 137/637 (21.51%), Postives = 226/637 (35.48%), Query Frame = 0

Query: 23  DLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCKVIAFVTTPNFTE----NPLEKG 82
           DL+  SW A                +  RY+ E +   V+ F   P+F+E    +P  K 
Sbjct: 12  DLITRSWSA-----------SKQAYLTERYHKEEAGA-VVIFAFQPSFSEKDFFDPDNKS 71

Query: 83  GGDLVPLSGLNDAFTAFEFLCSK--GCSDSA-INGAALDLFSPFY--HLYEQEILRLISN 142
               + L+ +       +F C +  G  D A +N A L             Q  + +   
Sbjct: 72  SFGEIKLNRV-------QFPCMRKIGKGDVATVNEAFLKNLEAVIDPRTSFQASVEMAVR 131

Query: 143 PNTQLIITGHALGGWVASLFTLLLLDLLDLTKTK---RPLCITYGSPLLGNEGLQKAISK 202
              Q++ TGH+ GG  A L T+  L+   +        P C+T+G+PL+G+     A+ +
Sbjct: 132 SRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGR 191

Query: 203 FSTWSSCFLHVVSNHDPLPR---------------------------------------- 262
              WS  F++ V+  D +PR                                        
Sbjct: 192 -EKWSRFFVNFVTRFDIVPRITLARKASVEETLPHVLAQLDPRNSSVQESEQRITEFYTS 251

Query: 263 ----------------------------RFLNHRGYYPFGTFLFCSDSGGCACSEDPTSI 322
                                        FL    Y P GTF+F ++    A + +  +I
Sbjct: 252 VMRDTSTVANQAVCELTGSAEAILETLSSFLELSPYRPAGTFVFSTEKRLVAVN-NSDAI 311

Query: 323 LKLLEATAVQNNPLGSLLFDYK-----ETIDRLKNRVNLKIVTELITENTESLRGSFMAQ 382
           L++L  T   ++     L  ++      + + L   + +K+   L  EN      S  + 
Sbjct: 312 LQMLFYTCQASDEQEWSLIPFRSIRDHHSYEELVQSMGMKLFNHLDGEN------SIESS 371

Query: 383 LDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 442
           L+ +GV     Q  Q           +  E   + ++ K     +    +K +    + Y
Sbjct: 372 LNDLGVSTRGRQYVQ---------AALEEEKKRVENQKKIIQVIQQERFLKKLAWIEDEY 431

Query: 443 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 502
           K KC++   GYYDS+K ++ E D +    +  L   ++ ++   K+    +    +  W+
Sbjct: 432 KPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLLKKCQLPDEFEGDIDWI 491

Query: 503 GGGTNYRRMVEPLDIAEHYRKGLN--------------------NYESHRPKHYKQLEE- 546
              T YRR+VEPLDIA ++R   N                     YE H  K    + E 
Sbjct: 492 KLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHHILKPNGMIAED 551

BLAST of Spg010932 vs. ExPASy Swiss-Prot
Match: Q9SU72 (Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.4e-14
Identity = 135/636 (21.23%), Postives = 224/636 (35.22%), Query Frame = 0

Query: 23  DLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCKVIAFVTTPNFTE----NPLEKG 82
           DL+  SW A                +  RY+ E +   V+ F   P+F+E    +P  K 
Sbjct: 12  DLITRSWSA-----------SKQAYLTERYHKEEAGA-VVIFAFQPSFSEKDFFDPDNKS 71

Query: 83  GGDLVPLSGLNDAFTAFEFLCSK--GCSDSA-INGAALDLFSPFY--HLYEQEILRLISN 142
               + L+ +       +F C +  G  D A +N A L             Q  + +   
Sbjct: 72  SFGEIKLNRV-------QFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVR 131

Query: 143 PNTQLIITGHALGGWVASLFTLLLLDLLDLTKTK---RPLCITYGSPLLGNEGLQKAISK 202
              Q++ TGH+ GG  A L T+  L+   +        P C+T+G+PL+G+     A+ +
Sbjct: 132 SRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGR 191

Query: 203 FSTWSSCFLHVVSNHDPLPR---------------------------------------- 262
              WS  F++ VS  D +PR                                        
Sbjct: 192 -EKWSRFFVNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTR 251

Query: 263 ----------------------------RFLNHRGYYPFGTFLFCSDSGGCACSEDPTSI 322
                                        FL    Y P GTF+F ++    A + +  +I
Sbjct: 252 VMRDTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVN-NSDAI 311

Query: 323 LKLLEATAVQNNPLGSLLFDYK-----ETIDRLKNRVNLKIVTELITENTESLRGSFMAQ 382
           L++L  T+  ++     L  ++      + + L   +  K+   L  EN      S  + 
Sbjct: 312 LQMLFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGEN------SIEST 371

Query: 383 LDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 442
           L+ +GV     Q  Q           +  E   + ++ K     +    +K +    + Y
Sbjct: 372 LNDLGVSTRGRQYVQ---------AALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEY 431

Query: 443 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 502
           K KC++   GYYDS+K ++ E D +    +  L   ++ ++   K+    +    +  W+
Sbjct: 432 KPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWI 491

Query: 503 GGGTNYRRMVEPLDIAEHYRKGLNN-----YESHRPKHYKQLEEWLEESTSNSSTTQRES 546
              T YRR+VEPLDIA ++R   N       +  RP  Y   +   E      +    E 
Sbjct: 492 KLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAED 551

BLAST of Spg010932 vs. ExPASy TrEMBL
Match: A0A6J1DAC5 (senescence-associated carboxylesterase 101 OS=Momordica charantia OX=3673 GN=LOC111018337 PE=4 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.8e-241
Identity = 427/584 (73.12%), Postives = 487/584 (83.39%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           MDD+NS RFSSGLELAS IATSDLLEHSW+AITK RS+I+AN      F   YNERSNC+
Sbjct: 1   MDDENSLRFSSGLELASFIATSDLLEHSWRAITKQRSKISANGSRFSAFFGRYNERSNCE 60

Query: 61  VIAFVTTPNFTENPLE-KGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLFSPF 120
           +IAFVT PN  ENPLE + GGDLV  S   D F AFEFLCSK CSD AINGAAL+LF  F
Sbjct: 61  IIAFVTAPNCRENPLEGERGGDLVSFSDFKDVFPAFEFLCSKQCSDFAINGAALELFWDF 120

Query: 121 YHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPLL 180
           Y +YEQEI+RL SNP TQLIITG ALGG VASLFTL LLD LDLTK KRPLCIT+GSPLL
Sbjct: 121 YDVYEQEIVRLKSNPKTQLIITGDALGGSVASLFTLFLLDFLDLTKAKRPLCITFGSPLL 180

Query: 181 GNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSEDP 240
           G+E LQ AIS+FSTWSSCFLHVVSNHDPLPR FLNH  YYPFGTFLFCS+S GCACS DP
Sbjct: 181 GDEALQSAISQFSTWSSCFLHVVSNHDPLPRMFLNHTAYYPFGTFLFCSESDGCACSGDP 240

Query: 241 TSILKLLEATAVQN--NPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQ 300
            ++ KLLEATAV N  +   SLLFDYKET+D L N+ NLK ++ LITENTESLRGSF+AQ
Sbjct: 241 ATVSKLLEATAVPNVTSNSHSLLFDYKETVDHLINQANLKAMSMLITENTESLRGSFVAQ 300

Query: 301 LDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 360
           L+AIGVV+ QAQ+ QN+DVN+L   +I  E D I + TK SDPS+ LN +KI MA LEWY
Sbjct: 301 LEAIGVVQIQAQQQQNVDVNKLVKKMIERENDTILE-TKTSDPSEALNMVKINMAKLEWY 360

Query: 361 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 420
           KKKC+SD+IGYYDSYKN  +  DIRVAEFKK+LRVYWE +VEEA+R+PQREGAPFN RW+
Sbjct: 361 KKKCQSDDIGYYDSYKNQRHPPDIRVAEFKKKLRVYWEKMVEEAERRPQREGAPFNIRWM 420

Query: 421 GGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEESTSNSSTTQRESVSSIL 480
            GGTNYRRMVEPLDIAEHYRKGL +Y+SHRPKHY++LEEW EES+S+ ST Q+ESVSSIL
Sbjct: 421 FGGTNYRRMVEPLDIAEHYRKGLRDYKSHRPKHYEKLEEWFEESSSDPSTGQKESVSSIL 480

Query: 481 TVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSEIFLP 540
           TVDSCFWA+VEEAILACQV+K+V+CSA + E A+ KL  FEDYVV +M+N AVSSEIFLP
Sbjct: 481 TVDSCFWANVEEAILACQVVKMVDCSAEEEEEAMGKLKDFEDYVVRMMKNSAVSSEIFLP 540

Query: 541 NSSFMTWWKEYDTIMGDDHDSVLTRLMRNE-QYEEYANGRLEIP 581
            SSFM WW++YD I+GDDHDS+L+ LMRNE  YE+YANGRLEIP
Sbjct: 541 QSSFMRWWEQYDEIVGDDHDSILSVLMRNEDHYEQYANGRLEIP 583

BLAST of Spg010932 vs. ExPASy TrEMBL
Match: A0A6J1J8J7 (senescence-associated carboxylesterase 101 OS=Cucurbita maxima OX=3661 GN=LOC111482262 PE=4 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 2.7e-234
Identity = 416/584 (71.23%), Postives = 480/584 (82.19%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           M D+N HRFSSGLE+A+ IATSDLL+HSWKAITK+R+ ITA++  CL F  +YNERSNCK
Sbjct: 1   MADNNPHRFSSGLEIANLIATSDLLDHSWKAITKLRTVITADESKCLAFFGHYNERSNCK 60

Query: 61  VIAFVTTPNFTENPL-EKGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLFSPF 120
           ++AFVTTPN +ENPL E+GG DLV LS L D FTAFEFL S+ CSDSAINGAALDLF PF
Sbjct: 61  ILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCSDSAINGAALDLFGPF 120

Query: 121 YHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPLL 180
           Y +YEQEI RL SNP TQLIITGH LGG VASLFTLLLLD +DLTK KRPLCIT+GSPLL
Sbjct: 121 YPIYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLTKAKRPLCITFGSPLL 180

Query: 181 GNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSEDP 240
           GNE LQ AIS+FSTWSSCFLHVVSN DPLP   LN++ YYPFGTFLFCS+SGGC+C EDP
Sbjct: 181 GNEALQSAISQFSTWSSCFLHVVSNRDPLPTSLLNNKAYYPFGTFLFCSESGGCSCFEDP 240

Query: 241 TSILKLLEATAVQNNPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQLD 300
            S LKLLEAT    +   S  FDYKETI RL ++ NLK  T LITEN+ S   SF+AQL+
Sbjct: 241 KSTLKLLEATKANTHLTASSFFDYKETIHRLIDQANLKATTRLITENSVSRTASFIAQLE 300

Query: 301 AIGVVKNQAQRYQN--LDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 360
           AIGVV +QAQ+ Q   +DV RLA  L + EM+MIF+KT +S  +KTLND+KI MA LEWY
Sbjct: 301 AIGVVPDQAQQQQQRVVDVKRLAVELKDNEMNMIFEKTNSS--AKTLNDVKINMAKLEWY 360

Query: 361 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 420
           KKKC S++IGYYDSYKN  +E DI+VAEFKK L VYWEN VEEA+RKP +EG+P N R L
Sbjct: 361 KKKCNSESIGYYDSYKNGRSEPDIKVAEFKKILLVYWENKVEEAERKPVKEGSPLNVRLL 420

Query: 421 GGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEESTS-NSSTTQRESVSSI 480
            GGTNYRRMVEPLDIAEHYRKGL NY++HRPKHY++LEEW  EST+  SS+TQRESVSSI
Sbjct: 421 FGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNESTTPESSSTQRESVSSI 480

Query: 481 LTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSEIFL 540
           LTVDS FW HVEEA++ACQVL+ V+ SA ++E  +AKL  FE+YVVELM++Y VSSEIFL
Sbjct: 481 LTVDSLFWEHVEEAVVACQVLRTVDSSAEEKEAELAKLKEFEEYVVELMKSYGVSSEIFL 540

Query: 541 PNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGRLEIP 581
           P SSFM WW EYD I+GD HDSVLT LMRNE+Y+EYANGRL+IP
Sbjct: 541 PQSSFMRWWDEYDGIVGDGHDSVLTMLMRNEEYKEYANGRLDIP 582

BLAST of Spg010932 vs. ExPASy TrEMBL
Match: A0A6J1F5N9 (senescence-associated carboxylesterase 101 OS=Cucurbita moschata OX=3662 GN=LOC111442342 PE=4 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 7.2e-232
Identity = 414/584 (70.89%), Postives = 475/584 (81.34%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           M D+N  RFS+GLE+A+ IATSDLL+HSWKAITK+R+ ITA++  CL F  +YNERSNCK
Sbjct: 1   MADNNPQRFSNGLEIANLIATSDLLDHSWKAITKLRTVITADESKCLAFFGHYNERSNCK 60

Query: 61  VIAFVTTPNFTENPL-EKGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLFSPF 120
           ++AFVTTPN +ENPL E+GG DLV LS L D FTAFEFL S+ CSDSAINGAALDLF  F
Sbjct: 61  ILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCSDSAINGAALDLFGRF 120

Query: 121 YHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPLL 180
           Y +YEQEI RL SNP TQLIITGH LGG VASLFTLLLLD +DLTK KRPLCIT+GSPLL
Sbjct: 121 YPVYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLTKAKRPLCITFGSPLL 180

Query: 181 GNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSEDP 240
           GNE LQ AIS+FSTWSSCFLHVVSN DPLP   LN++ YYPFGTFLFCS+SGGC+C EDP
Sbjct: 181 GNEALQSAISQFSTWSSCFLHVVSNRDPLPTSLLNNKAYYPFGTFLFCSESGGCSCFEDP 240

Query: 241 TSILKLLEATAVQNNPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQLD 300
            S  KLLEAT    +   S  FDYKETI RL ++ NLK  T LITEN+ES   SF+AQL+
Sbjct: 241 KSTSKLLEATKANTHLTASSFFDYKETIHRLIDQANLKATTRLITENSESWPASFIAQLE 300

Query: 301 AIGVVKNQAQRYQN--LDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 360
           AIGVV +QAQ+ Q   +DV RLA  L   EM+MIF+KT     +KTLND+KI MA LEWY
Sbjct: 301 AIGVVPDQAQQQQQRAVDVRRLAMALQGNEMNMIFEKTNPF--AKTLNDVKINMAKLEWY 360

Query: 361 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 420
           KKKC S++IGYYDSYKN   E DI+VAEFKK L VYWEN VEEA+RKP +EG+P N R L
Sbjct: 361 KKKCNSESIGYYDSYKNGRTEPDIKVAEFKKILLVYWENKVEEAERKPVKEGSPLNVRLL 420

Query: 421 GGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEES-TSNSSTTQRESVSSI 480
            GGTNYRRMVEPLDIAEHYRKGL NY++HRPKHY++LEEW  ES T  SS+TQRESVSSI
Sbjct: 421 FGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNESMTPESSSTQRESVSSI 480

Query: 481 LTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSEIFL 540
           LTVDS FWAHVEEA++ACQVL++V+ SA ++E  +AKL  FE+YVVELM+NY VSSEIFL
Sbjct: 481 LTVDSLFWAHVEEAVVACQVLRMVDSSAEEKEAELAKLKEFEEYVVELMKNYGVSSEIFL 540

Query: 541 PNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGRLEIP 581
           P SSFM WW EYD I+GD HDSVLT LMRNE+Y+EYANGRL+IP
Sbjct: 541 PQSSFMRWWDEYDGIVGDGHDSVLTMLMRNEEYKEYANGRLDIP 582

BLAST of Spg010932 vs. ExPASy TrEMBL
Match: A0A5D3D1Z0 (Senescence-associated carboxylesterase 101 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00280 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 3.1e-227
Identity = 409/587 (69.68%), Postives = 470/587 (80.07%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           MDD  SH+FSSGLEL + IATSDLLEHSW AITK RSEI    P+  V H Y NER NCK
Sbjct: 1   MDDHISHQFSSGLELGNFIATSDLLEHSWNAITKRRSEIQFTLPIYSVLHGYCNERLNCK 60

Query: 61  VIAFVTTPNFTENPLE-KGGGDLVPLSGL-NDAFTAFEFLCSKGCSDSAINGAALDLFSP 120
           ++AFVT PNFTENP+E  GG DLV +S +  D FTAFEFL S+ CSDSAING AL+LF  
Sbjct: 61  IVAFVTAPNFTENPIEGDGGEDLVLVSDMVKDEFTAFEFLQSRTCSDSAINGVALELFRR 120

Query: 121 FYHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPL 180
           FY +YEQEI RLISNPNTQLIITGH LGG VASLFTLLLLD +DLTKTKRPLCIT+GSPL
Sbjct: 121 FYPVYEQEISRLISNPNTQLIITGHGLGGSVASLFTLLLLDCIDLTKTKRPLCITFGSPL 180

Query: 181 LGNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSED 240
           LGNE  Q AIS FSTWSSCFLH+VSN DPLPR+ LN++ YYPFGTFLFCS SGGC+C ED
Sbjct: 181 LGNEAFQNAISHFSTWSSCFLHLVSNQDPLPRKLLNNKAYYPFGTFLFCSQSGGCSCFED 240

Query: 241 PTSILKLLEATAVQNNPL--GSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMA 300
           P SILK+LEAT   N+ L   S  FDYKETIDRL  + N K    +  EN ES  GSF+A
Sbjct: 241 PKSILKMLEATKANNDVLLNASAFFDYKETIDRLIKQTNFKANINVKIENAESWTGSFLA 300

Query: 301 QLDAIGVVKNQAQRYQN--LDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYL 360
           QL+AIGV +NQAQ+ Q   +D+NRLA  L + EM+MI + TK    +KTLND+KI MA L
Sbjct: 301 QLEAIGVAQNQAQQQQQRVVDINRLAKTLKDNEMNMILENTKPF--AKTLNDVKINMAKL 360

Query: 361 EWYKKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNT 420
           EWYKKKCK DNIGYYDSYKN  +E DIRVAEFKK L+VYWEN VEEA+RKP R+GAPF+ 
Sbjct: 361 EWYKKKCKFDNIGYYDSYKNQMSEPDIRVAEFKKILQVYWENKVEEAERKPLRDGAPFDV 420

Query: 421 RWLGGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEE-STSNSSTTQRESV 480
           + L GGTNYRRMVEPLDIAEHYRKGL +Y+SHRPKHY +LE+W ++  T +SS+ Q E+V
Sbjct: 421 KLLFGGTNYRRMVEPLDIAEHYRKGLKDYKSHRPKHYTKLEQWFKDLKTPDSSSMQEEAV 480

Query: 481 SSILTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSE 540
           SSILTVDS FW HVEEAILAC +++  +CS  +RE  +AKLT FEDYV+ELMRNYAVSSE
Sbjct: 481 SSILTVDSLFWVHVEEAILACDLVREGDCSEEEREAELAKLTEFEDYVLELMRNYAVSSE 540

Query: 541 IFLPNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGRLEIP 581
           IFLP+S+FM WW+EYD I+GDDHDSVL+ LMRNE+YEEY NGRL IP
Sbjct: 541 IFLPHSTFMKWWEEYDEIVGDDHDSVLSGLMRNEEYEEYGNGRLVIP 585

BLAST of Spg010932 vs. ExPASy TrEMBL
Match: A0A1S3B7N2 (senescence-associated carboxylesterase 101 OS=Cucumis melo OX=3656 GN=LOC103487057 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 3.1e-227
Identity = 409/587 (69.68%), Postives = 470/587 (80.07%), Query Frame = 0

Query: 1   MDDDNSHRFSSGLELASSIATSDLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCK 60
           MDD  SH+FSSGLEL + IATSDLLEHSW AITK RSEI    P+  V H Y NER NCK
Sbjct: 1   MDDHISHQFSSGLELGNFIATSDLLEHSWNAITKRRSEIQFTLPIYSVLHGYCNERLNCK 60

Query: 61  VIAFVTTPNFTENPLE-KGGGDLVPLSGL-NDAFTAFEFLCSKGCSDSAINGAALDLFSP 120
           ++AFVT PNFTENP+E  GG DLV +S +  D FTAFEFL S+ CSDSAING AL+LF  
Sbjct: 61  IVAFVTAPNFTENPIEGDGGEDLVLVSDMVKDEFTAFEFLQSRTCSDSAINGVALELFRR 120

Query: 121 FYHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPL 180
           FY +YEQEI RLISNPNTQLIITGH LGG VASLFTLLLLD +DLTKTKRPLCIT+GSPL
Sbjct: 121 FYPVYEQEISRLISNPNTQLIITGHGLGGSVASLFTLLLLDCIDLTKTKRPLCITFGSPL 180

Query: 181 LGNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSED 240
           LGNE  Q AIS FSTWSSCFLH+VSN DPLPR+ LN++ YYPFGTFLFCS SGGC+C ED
Sbjct: 181 LGNEAFQNAISHFSTWSSCFLHLVSNQDPLPRKLLNNKAYYPFGTFLFCSQSGGCSCFED 240

Query: 241 PTSILKLLEATAVQNNPL--GSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMA 300
           P SILK+LEAT   N+ L   S  FDYKETIDRL  + N K    +  EN ES  GSF+A
Sbjct: 241 PKSILKMLEATKANNDVLLNASAFFDYKETIDRLIKQTNFKANINVKIENAESWTGSFLA 300

Query: 301 QLDAIGVVKNQAQRYQN--LDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYL 360
           QL+AIGV +NQAQ+ Q   +D+NRLA  L + EM+MI + TK    +KTLND+KI MA L
Sbjct: 301 QLEAIGVAQNQAQQQQQRVVDINRLAKTLKDNEMNMILENTKPF--AKTLNDVKINMAKL 360

Query: 361 EWYKKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNT 420
           EWYKKKCK DNIGYYDSYKN  +E DIRVAEFKK L+VYWEN VEEA+RKP R+GAPF+ 
Sbjct: 361 EWYKKKCKFDNIGYYDSYKNQMSEPDIRVAEFKKILQVYWENKVEEAERKPLRDGAPFDV 420

Query: 421 RWLGGGTNYRRMVEPLDIAEHYRKGLNNYESHRPKHYKQLEEWLEE-STSNSSTTQRESV 480
           + L GGTNYRRMVEPLDIAEHYRKGL +Y+SHRPKHY +LE+W ++  T +SS+ Q E+V
Sbjct: 421 KLLFGGTNYRRMVEPLDIAEHYRKGLKDYKSHRPKHYTKLEQWFKDLKTPDSSSMQEEAV 480

Query: 481 SSILTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMRNYAVSSE 540
           SSILTVDS FW HVEEAILAC +++  +CS  +RE  +AKLT FEDYV+ELMRNYAVSSE
Sbjct: 481 SSILTVDSLFWVHVEEAILACDLVREGDCSEEEREAELAKLTEFEDYVLELMRNYAVSSE 540

Query: 541 IFLPNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGRLEIP 581
           IFLP+S+FM WW+EYD I+GDDHDSVL+ LMRNE+YEEY NGRL IP
Sbjct: 541 IFLPHSTFMKWWEEYDEIVGDDHDSVLSGLMRNEEYEEYGNGRLVIP 585

BLAST of Spg010932 vs. TAIR 10
Match: AT5G14930.2 (senescence-associated gene 101 )

HSP 1 Score: 303.1 bits (775), Expect = 4.7e-82
Identity = 214/589 (36.33%), Postives = 295/589 (50.08%), Query Frame = 0

Query: 12  GLELASSIATSDLLEHSWKAITKVRSEITAN-----------KPMCLVFHRYYNERSNCK 71
           G  L   + TS LL  SW  I ++ +   +N           K   L F  +  E+    
Sbjct: 9   GSALGKLVVTSGLLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLV 68

Query: 72  VIAFVTTPNFTENPLEKGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLF---S 131
           V        F+  P+ +       L  + D    F FLCS+     +++  A +LF   S
Sbjct: 69  V--------FSAPPICRSSSSDSTLLHVKDKENPFPFLCSENNPSFSLHTPAFNLFTSAS 128

Query: 132 PFYHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSP 191
                 + E+L+ + +    +IITG ALGG VASL+TL LL+ ++ T  KRPLCIT+GSP
Sbjct: 129 TSLTYLKSELLQTLKS-EKPVIITGAALGGSVASLYTLWLLETIEPT-LKRPLCITFGSP 188

Query: 192 LLGNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSE 251
           L+G+  LQ+ +   S  +SCFLHVVS    +   F     + PFGTFL C DS GC C E
Sbjct: 189 LIGDASLQQILEN-SVRNSCFLHVVSAQTRIKMDF-----FKPFGTFLICFDS-GCVCIE 248

Query: 252 DPTSILKLLEATAVQNNPLGSLLFDYKETIDRLKNRVNLKIVTELITENTESLRGSFMAQ 311
           D  ++ +LL      N    S L DY + ++RL              ++  SL  S +  
Sbjct: 249 DHVAVTELL------NGVHDSGLVDYSQVLNRL-------------DQSMLSLADSRLIP 308

Query: 312 LDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 371
            D I  ++ +A+             + N   DM+F         K LND+KI MAY+EWY
Sbjct: 309 EDVIKGIEKRAE-------------MKNLRFDMMF---------KKLNDMKISMAYIEWY 368

Query: 372 KKKCKSDNIGYYDSYKN----ASNEFDIRVA-EFKKRLRVYWENIVEEAKRKPQREGAPF 431
           KKKCK   IGYYD +K      S EFDI +    K  L  +W+++VEE +R+PQ + +  
Sbjct: 369 KKKCKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASIL 428

Query: 432 NTRWLGGGTNYRRMVEPLDIAEHYRKGLNNYE-SHRPKHYKQLEEW--LEESTSNSSTTQ 491
             R+L  G NYRRM+EPLDIAE+Y +G   Y  + R  HY  LE+W  +E         +
Sbjct: 429 KRRFLFSGNNYRRMIEPLDIAEYYLEGRKEYRTTGRSHHYVMLEKWFGMESILIEKERCK 488

Query: 492 RESVSSILTVDSCFWAHVEEAILACQVLKIVNCSARD--REVAVAKLTTFEDYVVELMRN 551
           +  +S +LT DSCFWA VE++++    L       RD  REV   KL  FE YV E++  
Sbjct: 489 KRDLSDLLTFDSCFWAEVEDSLIVINQLN-TTVGMRDDVREVLTRKLVEFEGYVWEIITK 537

Query: 552 YAVSSEIFLPNSSFMTWWKEYDTIMGDDHDSVLTRLMRNEQYEEYANGR 577
             VS EIFL  SSFM WWKEY  I G  + S LT  M   +YE Y   +
Sbjct: 549 REVSPEIFLEESSFMKWWKEYKKIKG-FNSSYLTEFMNTRKYESYGKSQ 537

BLAST of Spg010932 vs. TAIR 10
Match: AT3G52430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 160.6 bits (405), Expect = 3.8e-39
Identity = 127/454 (27.97%), Postives = 213/454 (46.92%), Query Frame = 0

Query: 119 FYHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSPL 178
           F  L  +E L L       ++ITGH+ GG +A+ FT L L       + R  CIT+GSPL
Sbjct: 93  FLQLKIKEGLELELLGKKLVVITGHSTGGALAA-FTALWLLSQSSPPSFRVFCITFGSPL 152

Query: 179 LGNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSED 238
           LGN+ L  +IS+ S  +  F HVVS HD +PR   ++  ++PFGT+LFCSD GG  C ++
Sbjct: 153 LGNQSLSTSISR-SRLAHNFCHVVSIHDLVPRS--SNEQFWPFGTYLFCSDKGG-VCLDN 212

Query: 239 PTSI---LKLLEATAVQNNPLGSLLFDYKETIDR--LKNRVNLKIVTELITENTESLRGS 298
             S+     +L  TA QN         Y  T+    LK+R     +   I +N  S +  
Sbjct: 213 AGSVRLMFNILNTTATQNTEEHQRYGHYVFTLSHMFLKSR---SFLGGSIPDN--SYQAG 272

Query: 299 FMAQLDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAY 358
               ++A+G   +        +    AT ++   +       ++++ +  L  +      
Sbjct: 273 VALAVEALGFSNDDTSGVLVKECIETATRIVRAPI------LRSAELANELASVLPARLE 332

Query: 359 LEWYKKKC--KSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAP 418
           ++WYK +C    + +GYYD +K  S + D +V   + RL  +W+ +++  +         
Sbjct: 333 IQWYKDRCDASEEQLGYYDFFKRYSLKRDFKVNMSRIRLAKFWDTVIKMVETNELPFDFH 392

Query: 419 FNTRWLGGGTNYRRMVEPLDIAEHYRK-----GLNNYESHRPKHYKQLEEWLEESTSNSS 478
              +W+     Y+ + EPLDIA  Y+      G +  E +RPK Y+ +++W +       
Sbjct: 393 LGKKWIYASQFYQLLAEPLDIANFYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEE 452

Query: 479 TTQRESVSSILTVDSCFWAHVEEAILACQVLKIVNCSARDREVAVAKLTTFEDYVVELMR 538
             +    S+  T D+CFWA +E+A       +  +   + R +   K+  FE Y   L+ 
Sbjct: 453 CVRSRYAST--TQDTCFWAKLEQAKEWLDEARKESSDPQRRSLLREKIVPFESYANTLVT 512

Query: 539 NYAVSSEIFLPNSSFMTW---WKEYDTIMGDDHD 558
              VS ++   NSS+  W    KE+   MG +++
Sbjct: 513 KKEVSLDVKAKNSSYSVWEANLKEFKCKMGYENE 528

BLAST of Spg010932 vs. TAIR 10
Match: AT5G14930.3 (senescence-associated gene 101 )

HSP 1 Score: 116.7 bits (291), Expect = 6.2e-26
Identity = 85/248 (34.27%), Postives = 123/248 (49.60%), Query Frame = 0

Query: 12  GLELASSIATSDLLEHSWKAITKVRSEITAN-----------KPMCLVFHRYYNERSNCK 71
           G  L   + TS LL  SW  I ++ +   +N           K   L F  +  E+    
Sbjct: 9   GSALGKLVVTSGLLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLV 68

Query: 72  VIAFVTTPNFTENPLEKGGGDLVPLSGLNDAFTAFEFLCSKGCSDSAINGAALDLF---S 131
           V        F+  P+ +       L  + D    F FLCS+     +++  A +LF   S
Sbjct: 69  V--------FSAPPICRSSSSDSTLLHVKDKENPFPFLCSENNPSFSLHTPAFNLFTSAS 128

Query: 132 PFYHLYEQEILRLISNPNTQLIITGHALGGWVASLFTLLLLDLLDLTKTKRPLCITYGSP 191
                 + E+L+ + +    +IITG ALGG VASL+TL LL+ ++ T  KRPLCIT+GSP
Sbjct: 129 TSLTYLKSELLQTLKS-EKPVIITGAALGGSVASLYTLWLLETIEPT-LKRPLCITFGSP 188

Query: 192 LLGNEGLQKAISKFSTWSSCFLHVVSNHDPLPRRFLNHRGYYPFGTFLFCSDSGGCACSE 246
           L+G+  LQ+ +   S  +SCFLHVVS    +   F     + PFGTFL C DS GC C E
Sbjct: 189 LIGDASLQQILEN-SVRNSCFLHVVSAQTRIKMDF-----FKPFGTFLICFDS-GCVCIE 239

BLAST of Spg010932 vs. TAIR 10
Match: AT3G48080.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 9.0e-17
Identity = 107/514 (20.82%), Postives = 189/514 (36.77%), Query Frame = 0

Query: 137 QLIITGHALGGWVASLFTLLLLD---LLDLTKTKRPLCITYGSPLLGNEGLQKAISKFST 196
           Q++ TGH+ GG  A L T+  L+   + D      P C+T+G+PL+G+   + A+ +   
Sbjct: 116 QVVFTGHSFGGATAILATVWYLETYFIRDAYAAPEPRCVTFGAPLVGDYIFKHALGR-EN 175

Query: 197 WSSCFLHVVSNHDPLPRRFLNHR------------------------------------- 256
           WS  F++ V+  D +PR  L  +                                     
Sbjct: 176 WSRFFVNFVTRFDIVPRIMLARKTTIEQTLSYVLGKLDSTRAPIHESDQVITEFYTRVMR 235

Query: 257 -------------------------------GYYPFGTFLFCSDSGGCACSEDPTSILKL 316
                                           Y P GTF+F S         +  +IL++
Sbjct: 236 DTYTVASKAVCQLIGNGEAFLETLSSFYELSPYRPVGTFVF-STQKRLVVVNNSDAILQM 295

Query: 317 LEATAVQNNPLGSLLFDYKETID-----RLKNRVNLKIVTELITENTESLRGSFMAQLDA 376
           L  T   N+     +  +    D      L   + +K++  L       L    +   ++
Sbjct: 296 LFYTCQSNDEQELSVIPFLSIRDHHGYEELVQSIGIKLLNHL------DLHNPLLDGENS 355

Query: 377 IGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLND--IKIMMAYLEWYK 436
           IG          +L ++  A   I+  ++    + +     +T  D  ++ +   +E YK
Sbjct: 356 IG------SALDDLGMSTRARQCIHAALEAEKQRVENQKKIETKRDQIVERLTWIVEVYK 415

Query: 437 KKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWLG 496
            KC++   GYYDS+K+++ E D +    +  L   ++ ++   K+    +G   +  W+ 
Sbjct: 416 PKCQAHKNGYYDSFKDSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDGFEGSRGWIN 475

Query: 497 GGTNYRRMVEPLDIAEHYRKGLNN----YESH-RPKHYKQLEEWLE-------------- 546
             T YRR++EPLDI+ ++ +  N     Y  H RP  YK  +   E              
Sbjct: 476 LATQYRRLIEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILKPTGMIAKDV 535

BLAST of Spg010932 vs. TAIR 10
Match: AT3G48090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 1.0e-15
Identity = 135/636 (21.23%), Postives = 224/636 (35.22%), Query Frame = 0

Query: 23  DLLEHSWKAITKVRSEITANKPMCLVFHRYYNERSNCKVIAFVTTPNFTE----NPLEKG 82
           DL+  SW A                +  RY+ E +   V+ F   P+F+E    +P  K 
Sbjct: 12  DLITRSWSA-----------SKQAYLTERYHKEEAGA-VVIFAFQPSFSEKDFFDPDNKS 71

Query: 83  GGDLVPLSGLNDAFTAFEFLCSK--GCSDSA-INGAALDLFSPFY--HLYEQEILRLISN 142
               + L+ +       +F C +  G  D A +N A L             Q  + +   
Sbjct: 72  SFGEIKLNRV-------QFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVR 131

Query: 143 PNTQLIITGHALGGWVASLFTLLLLDLLDLTKTK---RPLCITYGSPLLGNEGLQKAISK 202
              Q++ TGH+ GG  A L T+  L+   +        P C+T+G+PL+G+     A+ +
Sbjct: 132 SRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGR 191

Query: 203 FSTWSSCFLHVVSNHDPLPR---------------------------------------- 262
              WS  F++ VS  D +PR                                        
Sbjct: 192 -EKWSRFFVNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTR 251

Query: 263 ----------------------------RFLNHRGYYPFGTFLFCSDSGGCACSEDPTSI 322
                                        FL    Y P GTF+F ++    A + +  +I
Sbjct: 252 VMRDTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVN-NSDAI 311

Query: 323 LKLLEATAVQNNPLGSLLFDYK-----ETIDRLKNRVNLKIVTELITENTESLRGSFMAQ 382
           L++L  T+  ++     L  ++      + + L   +  K+   L  EN      S  + 
Sbjct: 312 LQMLFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGEN------SIEST 371

Query: 383 LDAIGVVKNQAQRYQNLDVNRLATMLINCEMDMIFDKTKASDPSKTLNDIKIMMAYLEWY 442
           L+ +GV     Q  Q           +  E   + ++ K     +    +K +    + Y
Sbjct: 372 LNDLGVSTRGRQYVQ---------AALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEY 431

Query: 443 KKKCKSDNIGYYDSYKNASNEFDIRVAEFKKRLRVYWENIVEEAKRKPQREGAPFNTRWL 502
           K KC++   GYYDS+K ++ E D +    +  L   ++ ++   K+    +    +  W+
Sbjct: 432 KPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWI 491

Query: 503 GGGTNYRRMVEPLDIAEHYRKGLNN-----YESHRPKHYKQLEEWLEESTSNSSTTQRES 546
              T YRR+VEPLDIA ++R   N       +  RP  Y   +   E      +    E 
Sbjct: 492 KLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAED 551