Spg010882 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg010882 vs. NCBI nr
Match: XP_023526958.1 (senescence-associated carboxylesterase 101 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691.8 bits (1784), Expect = 4.6e-195
Identity = 358/539 (66.42%), Postives = 407/539 (75.51%), Query Frame = 0

Query: 2   CLAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCS 61
           CLAF+GHY+ERSNC+I+AFVTTPNC+ENPLEEEGG DLVSLSDLKDEFTAFEFL S+ C 
Sbjct: 46  CLAFFGHYNERSNCKILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCP 105

Query: 62  DSAINGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPT 121
           DSAINGAALDLF RFY   EQEI  L SN  TQ IITGH LGG +ASLFTLLLLD +D T
Sbjct: 106 DSAINGAALDLFGRFYPVYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLT 165

Query: 122 KP--PLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTF 181
           K   PLCIT+GSPLLGN+ LQ AIS+ S W S FLHVVSNHDPLP   LN++ YYPFGTF
Sbjct: 166 KAKRPLCITFGSPLLGNKALQSAISQFSTWSSCFLHVVSNHDPLPTSLLNNKAYYPFGTF 225

Query: 182 LFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSIT 241
           LFCSE GGC+C EDP ST KLLEAT    +  +   FDYKETI RLI+   LK TT+ IT
Sbjct: 226 LFCSESGGCSCFEDPKSTSKLLEATKANTHLTAFSFFDYKETILRLIDQANLKATTRLIT 285

Query: 242 KNTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPF- 301
           +N+ES   SFIAQ++AIG V +  QQ Q   +DV RLA  L  +EMNMIF K    NPF 
Sbjct: 286 ENSESWTASFIAQLEAIGVVPDQAQQQQQRAVDVKRLAMALQGNEMNMIFEKT---NPFA 345

Query: 302 KTLGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQ 361
           KTL D+KI MA +E Y ++  S +IGYYDSYKN   E D KVAE KK L VYWEN VE+ 
Sbjct: 346 KTLNDVKINMAKLEWYKKKCNSESIGYYDSYKNGRTEPDIKVAEFKKILLVYWENKVEEA 405

Query: 362 SRLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEE 421
            R   +        LLF GTNYRR +EPLDIAEHYRKGL NY++HRPKHYRKLEEW+ E 
Sbjct: 406 ERKPVKEGSPLNVRLLFGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNES 465

Query: 422 CAPHLT---KQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDY 481
             P  +   ++SV   LT DS FWAHVEEA++ CQ L+MV+ SA +KE  LAKL +FE+Y
Sbjct: 466 MTPESSSTQRESVSSILTVDSLFWAHVEEAVVACQVLRMVDSSAEEKEAELAKLKEFEEY 525

Query: 482 VVELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           VVELMKNY VSSEIFLPHS+FMRWW+EYD IVGDDHDSVLT LMRNEEY EYA G L+I
Sbjct: 526 VVELMKNYGVSSEIFLPHSSFMRWWDEYDGIVGDDHDSVLTMLMRNEEYKEYANGRLDI 581

BLAST of Spg010882 vs. NCBI nr
Match: XP_023526957.1 (senescence-associated carboxylesterase 101 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 690.3 bits (1780), Expect = 1.3e-194
Identity = 357/539 (66.23%), Postives = 406/539 (75.32%), Query Frame = 0

Query: 2   CLAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCS 61
           CLAF+GHY+ERSNC+I+AFVTTPNC+ENPLEEEGG DLVSLSDLKDEFTAFEFL S+ C 
Sbjct: 46  CLAFFGHYNERSNCKILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCP 105

Query: 62  DSAINGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPT 121
           DSAING ALDLF RFY   EQEI  L SN  TQ IITGH LGG +ASLFTLLLLD +D T
Sbjct: 106 DSAINGVALDLFGRFYPVYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLT 165

Query: 122 KP--PLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTF 181
           K   PLCIT+GSPLLGN+ LQ AIS+ S W S FLHVVSNHDPLP   LN++ YYPFGTF
Sbjct: 166 KAKRPLCITFGSPLLGNKALQSAISQFSTWSSCFLHVVSNHDPLPTSLLNNKAYYPFGTF 225

Query: 182 LFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSIT 241
           LFCSE GGC+C EDP ST KLLEAT    +  +   FDYKETI RLI+   LK TT+ IT
Sbjct: 226 LFCSESGGCSCFEDPKSTSKLLEATKANTHLTAFSFFDYKETILRLIDQANLKATTRLIT 285

Query: 242 KNTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPF- 301
           +N+ES   SFIAQ++AIG V +  QQ Q   +DV RLA  L  +EMNMIF K    NPF 
Sbjct: 286 ENSESWTASFIAQLEAIGVVPDQAQQQQQRAVDVKRLAMALQGNEMNMIFEKT---NPFA 345

Query: 302 KTLGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQ 361
           KTL D+KI MA +E Y ++  S +IGYYDSYKN   E D KVAE KK L VYWEN VE+ 
Sbjct: 346 KTLNDVKINMAKLEWYKKKCNSESIGYYDSYKNGRTEPDIKVAEFKKILLVYWENKVEEA 405

Query: 362 SRLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEE 421
            R   +        LLF GTNYRR +EPLDIAEHYRKGL NY++HRPKHYRKLEEW+ E 
Sbjct: 406 ERKPVKEGSPLNVRLLFGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNES 465

Query: 422 CAPHLT---KQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDY 481
             P  +   ++SV   LT DS FWAHVEEA++ CQ L+MV+ SA +KE  LAKL +FE+Y
Sbjct: 466 MTPESSSTQRESVSSILTVDSLFWAHVEEAVVACQVLRMVDSSAEEKEAELAKLKEFEEY 525

Query: 482 VVELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           VVELMKNY VSSEIFLPHS+FMRWW+EYD IVGDDHDSVLT LMRNEEY EYA G L+I
Sbjct: 526 VVELMKNYGVSSEIFLPHSSFMRWWDEYDGIVGDDHDSVLTMLMRNEEYKEYANGRLDI 581

BLAST of Spg010882 vs. NCBI nr
Match: XP_022935482.1 (senescence-associated carboxylesterase 101 [Cucurbita moschata] >KAG6581004.1 Senescence-associated carboxylesterase 101, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 689.5 bits (1778), Expect = 2.3e-194
Identity = 358/539 (66.42%), Postives = 406/539 (75.32%), Query Frame = 0

Query: 2   CLAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCS 61
           CLAF+GHY+ERSNC+I+AFVTTPNC+ENPLEEEGG DLVSLSDLKDEFTAFEFL S+ CS
Sbjct: 46  CLAFFGHYNERSNCKILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCS 105

Query: 62  DSAINGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPT 121
           DSAINGAALDLF RFY   EQEI  L SN  TQ IITGH LGG +ASLFTLLLLD +D T
Sbjct: 106 DSAINGAALDLFGRFYPVYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLT 165

Query: 122 KP--PLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTF 181
           K   PLCIT+GSPLLGNE LQ AIS+ S W S FLHVVSN DPLP   LN++ YYPFGTF
Sbjct: 166 KAKRPLCITFGSPLLGNEALQSAISQFSTWSSCFLHVVSNRDPLPTSLLNNKAYYPFGTF 225

Query: 182 LFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSIT 241
           LFCSE GGC+C EDP ST KLLEAT    +  +S  FDYKETI RLI+   LK TT+ IT
Sbjct: 226 LFCSESGGCSCFEDPKSTSKLLEATKANTHLTASSFFDYKETIHRLIDQANLKATTRLIT 285

Query: 242 KNTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPF- 301
           +N+ES   SFIAQ++AIG V +  QQ Q   +DV RLA  L  +EMNMIF K    NPF 
Sbjct: 286 ENSESWPASFIAQLEAIGVVPDQAQQQQQRAVDVRRLAMALQGNEMNMIFEKT---NPFA 345

Query: 302 KTLGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQ 361
           KTL D+KI MA +E Y ++  S +IGYYDSYKN   E D KVAE KK L VYWEN VE+ 
Sbjct: 346 KTLNDVKINMAKLEWYKKKCNSESIGYYDSYKNGRTEPDIKVAEFKKILLVYWENKVEEA 405

Query: 362 SRLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEE 421
            R   +        LLF GTNYRR +EPLDIAEHYRKGL NY++HRPKHYRKLEEW+ E 
Sbjct: 406 ERKPVKEGSPLNVRLLFGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNES 465

Query: 422 CAPHLT---KQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDY 481
             P  +   ++SV   LT DS FWAHVEEA++ CQ L+MV+ SA +KE  LAKL +FE+Y
Sbjct: 466 MTPESSSTQRESVSSILTVDSLFWAHVEEAVVACQVLRMVDSSAEEKEAELAKLKEFEEY 525

Query: 482 VVELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           VVELMKNY VSSEIFLP S+FMRWW+EYD IVGD HDSVLT LMRNEEY EYA G L+I
Sbjct: 526 VVELMKNYGVSSEIFLPQSSFMRWWDEYDGIVGDGHDSVLTMLMRNEEYKEYANGRLDI 581

BLAST of Spg010882 vs. NCBI nr
Match: XP_023526959.1 (senescence-associated carboxylesterase 101 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 683.7 bits (1763), Expect = 1.3e-192
Identity = 356/539 (66.05%), Postives = 405/539 (75.14%), Query Frame = 0

Query: 2   CLAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCS 61
           CLAF+GHY+ERSNC+I+AFVTTPNC+ENPL EEGG DLVSLSDLKDEFTAFEFL S+ C 
Sbjct: 46  CLAFFGHYNERSNCKILAFVTTPNCSENPL-EEGGEDLVSLSDLKDEFTAFEFLYSESCP 105

Query: 62  DSAINGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPT 121
           DSAING ALDLF RFY   EQEI  L SN  TQ IITGH LGG +ASLFTLLLLD +D T
Sbjct: 106 DSAINGVALDLFGRFYPVYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLT 165

Query: 122 KP--PLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTF 181
           K   PLCIT+GSPLLGN+ LQ AIS+ S W S FLHVVSNHDPLP   LN++ YYPFGTF
Sbjct: 166 KAKRPLCITFGSPLLGNKALQSAISQFSTWSSCFLHVVSNHDPLPTSLLNNKAYYPFGTF 225

Query: 182 LFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSIT 241
           LFCSE GGC+C EDP ST KLLEAT    +  +   FDYKETI RLI+   LK TT+ IT
Sbjct: 226 LFCSESGGCSCFEDPKSTSKLLEATKANTHLTAFSFFDYKETILRLIDQANLKATTRLIT 285

Query: 242 KNTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPF- 301
           +N+ES   SFIAQ++AIG V +  QQ Q   +DV RLA  L  +EMNMIF K    NPF 
Sbjct: 286 ENSESWTASFIAQLEAIGVVPDQAQQQQQRAVDVKRLAMALQGNEMNMIFEKT---NPFA 345

Query: 302 KTLGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQ 361
           KTL D+KI MA +E Y ++  S +IGYYDSYKN   E D KVAE KK L VYWEN VE+ 
Sbjct: 346 KTLNDVKINMAKLEWYKKKCNSESIGYYDSYKNGRTEPDIKVAEFKKILLVYWENKVEEA 405

Query: 362 SRLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEE 421
            R   +        LLF GTNYRR +EPLDIAEHYRKGL NY++HRPKHYRKLEEW+ E 
Sbjct: 406 ERKPVKEGSPLNVRLLFGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNES 465

Query: 422 CAPHLT---KQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDY 481
             P  +   ++SV   LT DS FWAHVEEA++ CQ L+MV+ SA +KE  LAKL +FE+Y
Sbjct: 466 MTPESSSTQRESVSSILTVDSLFWAHVEEAVVACQVLRMVDSSAEEKEAELAKLKEFEEY 525

Query: 482 VVELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           VVELMKNY VSSEIFLPHS+FMRWW+EYD IVGDDHDSVLT LMRNEEY EYA G L+I
Sbjct: 526 VVELMKNYGVSSEIFLPHSSFMRWWDEYDGIVGDDHDSVLTMLMRNEEYKEYANGRLDI 580

BLAST of Spg010882 vs. NCBI nr
Match: XP_022983733.1 (senescence-associated carboxylesterase 101 [Cucurbita maxima])

HSP 1 Score: 677.2 bits (1746), Expect = 1.2e-190
Identity = 351/538 (65.24%), Postives = 403/538 (74.91%), Query Frame = 0

Query: 2   CLAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCS 61
           CLAF+GHY+ERSNC+I+AFVTTPNC+ENPLEEEGG DLVSLSDLKDEFTAFEFL S+ CS
Sbjct: 46  CLAFFGHYNERSNCKILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCS 105

Query: 62  DSAINGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPT 121
           DSAINGAALDLF  FY   EQEI  L SN  TQ IITGH LGG +ASLFTLLLLD +D T
Sbjct: 106 DSAINGAALDLFGPFYPIYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLT 165

Query: 122 KP--PLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTF 181
           K   PLCIT+GSPLLGNE LQ AIS+ S W S FLHVVSN DPLP   LN++ YYPFGTF
Sbjct: 166 KAKRPLCITFGSPLLGNEALQSAISQFSTWSSCFLHVVSNRDPLPTSLLNNKAYYPFGTF 225

Query: 182 LFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSIT 241
           LFCSE GGC+C EDP STLKLLEAT    +  +S  FDYKETI RLI+   LK TT+ IT
Sbjct: 226 LFCSESGGCSCFEDPKSTLKLLEATKANTHLTASSFFDYKETIHRLIDQANLKATTRLIT 285

Query: 242 KNTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPFK 301
           +N+ S   SFIAQ++AIG V +  QQ Q   +DV RLA  L ++EMNMIF K    +  K
Sbjct: 286 ENSVSRTASFIAQLEAIGVVPDQAQQQQQRVVDVKRLAVELKDNEMNMIFEK--TNSSAK 345

Query: 302 TLGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQS 361
           TL D+KI MA +E Y ++  S +IGYYDSYKN  +E D KVAE KK L VYWEN VE+  
Sbjct: 346 TLNDVKINMAKLEWYKKKCNSESIGYYDSYKNGRSEPDIKVAEFKKILLVYWENKVEEAE 405

Query: 362 RLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEEC 421
           R   +        LLF GTNYRR +EPLDIAEHYRKGL NY++HRPKHYRKLEEW+ E  
Sbjct: 406 RKPVKEGSPLNVRLLFGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNEST 465

Query: 422 APHLT---KQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDYV 481
            P  +   ++SV   LT DS FW HVEEA++ CQ L+ V+ SA +KE  LAKL +FE+YV
Sbjct: 466 TPESSSTQRESVSSILTVDSLFWEHVEEAVVACQVLRTVDSSAEEKEAELAKLKEFEEYV 525

Query: 482 VELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           VELMK+Y VSSEIFLP S+FMRWW+EYD IVGD HDSVLT LMRNEEY EYA G L+I
Sbjct: 526 VELMKSYGVSSEIFLPQSSFMRWWDEYDGIVGDGHDSVLTMLMRNEEYKEYANGRLDI 581

BLAST of Spg010882 vs. ExPASy Swiss-Prot
Match: Q4F883 (Senescence-associated carboxylesterase 101 OS=Arabidopsis thaliana OX=3702 GN=SAG101 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.7e-65
Identity = 190/541 (35.12%), Postives = 269/541 (49.72%), Query Frame = 0

Query: 3   LAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCSD 62
           L F  H +E+    ++ F   P C  +  +        +L  +KD+   F FLCS+    
Sbjct: 55  LEFQIHREEKFT--LVVFSAPPICRSSSSDS-------TLLHVKDKENPFPFLCSENNPS 114

Query: 63  SAINGAALDLF---CRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLD 122
            +++  A +LF        + + E+L  + +     IITG ALGG +ASL+TL LL+ ++
Sbjct: 115 FSLHTPAFNLFTSASTSLTYLKSELLQTLKSEK-PVIITGAALGGSVASLYTLWLLETIE 174

Query: 123 PT-KPPLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGT 182
           PT K PLCIT+GSPL+G+  LQ+ I E S   S FLHVVS    +   F     + PFGT
Sbjct: 175 PTLKRPLCITFGSPLIGDASLQQ-ILENSVRNSCFLHVVSAQTRIKMDF-----FKPFGT 234

Query: 183 FLFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSI 242
           FL C +  GC C ED  +  +LL      N    S L DY + ++RL             
Sbjct: 235 FLICFD-SGCVCIEDHVAVTELL------NGVHDSGLVDYSQVLNRL------------- 294

Query: 243 TKNTESLRGSFIAQIDAIGAVKNHVQQHQHIDVNRLASVLVNHEMNMIFGKIIAFNPFKT 302
            ++  SL  S +   D I  ++   +             + N   +M+         FK 
Sbjct: 295 DQSMLSLADSRLIPEDVIKGIEKRAE-------------MKNLRFDMM---------FKK 354

Query: 303 LGDLKIWMASIECY-QRYKSNNIGYYDSYKN----ASNELDFKVA-ELKKFLRVYWENVV 362
           L D+KI MA IE Y ++ K   IGYYD +K      S E D  +    K  L  +W++VV
Sbjct: 355 LNDMKISMAYIEWYKKKCKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVV 414

Query: 363 EQQSRLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYE-SHRPKHYRKLEEW 422
           E+  R  +  A I     LF+G NYRR IEPLDIAE+Y +G   Y  + R  HY  LE+W
Sbjct: 415 EEVERRPQSDASILKRRFLFSGNNYRRMIEPLDIAEYYLEGRKEYRTTGRSHHYVMLEKW 474

Query: 423 FEEEC----APHLTKQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARD--KEVVLAK 482
           F  E          K+ +   LT DSCFWA VE++++   +L       RD  +EV+  K
Sbjct: 475 FGMESILIEKERCKKRDLSDLLTFDSCFWAEVEDSLIVINQLN-TTVGMRDDVREVLTRK 534

Query: 483 LTKFEDYVVELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYA 527
           L +FE YV E++   +VS EIFL  S+FM+WW+EY +I G  + S LT  M   +Y  Y 
Sbjct: 535 LVEFEGYVWEIITKREVSPEIFLEESSFMKWWKEYKKIKG-FNSSYLTEFMNTRKYESYG 535

BLAST of Spg010882 vs. ExPASy Swiss-Prot
Match: Q9S745 (Lipase-like PAD4 OS=Arabidopsis thaliana OX=3702 GN=PAD4 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 8.9e-32
Identity = 132/490 (26.94%), Postives = 215/490 (43.88%), Query Frame = 0

Query: 65  INGAALDLF--CRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPTK 124
           ++ A L LF   +     E E+LG         +ITGH+ GG +A+   L LL    P  
Sbjct: 85  VDAAILKLFLQLKIKEGLELELLG-----KKLVVITGHSTGGALAAFTALWLLSQSSPPS 144

Query: 125 -PPLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTFLF 184
               CIT+GSPLLGN+ L  +IS  S+    F HVVS HD +PR   ++  ++PFGT+LF
Sbjct: 145 FRVFCITFGSPLLGNQSLSTSISR-SRLAHNFCHVVSIHDLVPRS--SNEQFWPFGTYLF 204

Query: 185 CSEFGGCACSEDPTST---LKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTT--- 244
           CS+ GG  C ++  S      +L  T  QN         Y  T+  +     LK  +   
Sbjct: 205 CSDKGG-VCLDNAGSVRLMFNILNTTATQNTEEHQRYGHYVFTLSHMF----LKSRSFLG 264

Query: 245 KSITKNTESLRGSFIAQIDAIG---------AVKNHVQQHQHI-DVNRLASVLVNHEMNM 304
            SI  N  S +      ++A+G          VK  ++    I     L S  + +E+  
Sbjct: 265 GSIPDN--SYQAGVALAVEALGFSNDDTSGVLVKECIETATRIVRAPILRSAELANELAS 324

Query: 305 IFGKIIAFNPFKTLGDLKIWMASIECYQRYKSNNIGYYDSYKNASNELDFKVAELKKFLR 364
           +    +    +K   D                  +GYYD +K  S + DFKV   +  L 
Sbjct: 325 VLPARLEIQWYKDRCDA-------------SEEQLGYYDFFKRYSLKRDFKVNMSRIRLA 384

Query: 365 VYWENVVEQQSRLSRRRAPI-----RLWYLLFAGTNYRRFIEPLDIAEHYRK-----GLN 424
            +W+ V++    +     P      + W  ++A   Y+   EPLDIA  Y+      G +
Sbjct: 385 KFWDTVIKM---VETNELPFDFHLGKKW--IYASQFYQLLAEPLDIANFYKNRDIKTGGH 444

Query: 425 NYESHRPKHYRKLEEWFEEECAPHLTKQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCS 484
             E +RPK Y  +++W +    P    +S   + T+D+CFWA +E+A     + +  +  
Sbjct: 445 YLEGNRPKRYEVIDKWQKGVKVPEECVRSRYASTTQDTCFWAKLEQAKEWLDEARKESSD 504

Query: 485 ARDKEVVLAKLTKFEDYVVELMKNYDVSSEIFLPHSTFMRW------------WEEYDEI 514
            + + ++  K+  FE Y   L+   +VS ++   +S++  W            +E   E+
Sbjct: 505 PQRRSLLREKIVPFESYANTLVTKKEVSLDVKAKNSSYSVWEANLKEFKCKMGYENEIEM 541

BLAST of Spg010882 vs. ExPASy Swiss-Prot
Match: Q9SU72 (Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.2e-09
Identity = 110/512 (21.48%), Postives = 181/512 (35.35%), Query Frame = 0

Query: 94  QYIITGHALGGWIASLFTLLLLDVLDPTKP-----PLCITYGSPLLGNEGLQKAISELSK 153
           Q + TGH+ GG  A L T+  L+      P     P C+T+G+PL+G+     A+    K
Sbjct: 116 QIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGR-EK 175

Query: 154 WPSFFLHVVSNHDPLPR------------------------------------------- 213
           W  FF++ VS  D +PR                                           
Sbjct: 176 WSRFFVNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMR 235

Query: 214 -------------------------RFLNHRGYYPFGTFLFCSEFGGCACSEDPTSTLKL 273
                                     FL    Y P GTF+F +E    A + +  + L++
Sbjct: 236 DTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVN-NSDAILQM 295

Query: 274 LEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSITKNTESLRGSFIAQIDAIGAVK 333
           L  T+  ++     L  ++   D       ++   K +  + +    S  + ++ +G V 
Sbjct: 296 LFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDG-ENSIESTLNDLG-VS 355

Query: 334 NHVQQHQHIDVNRLASVLVNHEMNMIFGKIIAFNPFKTLGDLKIWMASIECYQRYKSNNI 393
              +Q+    +      + N +      KII     +       W+   E   + +++  
Sbjct: 356 TRGRQYVQAALEEEKKRVENQK------KIIQVIEQERFLKKLAWIED-EYKPKCQAHKN 415

Query: 394 GYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQSRLSRRRAPIRL-----WYLLFAGT 453
           GYYDS+K ++ E DFK    +  L   ++ V+     + + + P        W  L   T
Sbjct: 416 GYYDSFKVSNEENDFKANVKRAELAGVFDEVL---GLMKKCQLPDEFEGDIDWIKL--AT 475

Query: 454 NYRRFIEPLDIAEHYRKGLNN-----YESHRPKHYRKLEEWFEEECAP------------ 498
            YRR +EPLDIA ++R   N       +  RP  Y   +  +E                 
Sbjct: 476 RYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWN 535

BLAST of Spg010882 vs. ExPASy Swiss-Prot
Match: Q9XF23 (Protein EDS1L OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.4e-08
Identity = 109/512 (21.29%), Postives = 178/512 (34.77%), Query Frame = 0

Query: 94  QYIITGHALGGWIASLFTLLLLDVLDPTKP-----PLCITYGSPLLGNEGLQKAISELSK 153
           Q + TGH+ GG  A L T+  L+      P     P C+T+G+PL+G+     A+    K
Sbjct: 116 QIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGR-EK 175

Query: 154 WPSFFLHVVSNHDPLPR------------------------------------------- 213
           W  FF++ V+  D +PR                                           
Sbjct: 176 WSRFFVNFVTRFDIVPRITLARKASVEETLPHVLAQLDPRNSSVQESEQRITEFYTSVMR 235

Query: 214 -------------------------RFLNHRGYYPFGTFLFCSEFGGCACSEDPTSTLKL 273
                                     FL    Y P GTF+F +E    A + +  + L++
Sbjct: 236 DTSTVANQAVCELTGSAEAILETLSSFLELSPYRPAGTFVFSTEKRLVAVN-NSDAILQM 295

Query: 274 LEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSITKNTESLRGSFIAQIDAIGAVK 333
           L  T   ++     L  ++   D       ++     +  + +    S  + ++ +G V 
Sbjct: 296 LFYTCQASDEQEWSLIPFRSIRDHHSYEELVQSMGMKLFNHLDG-ENSIESSLNDLG-VS 355

Query: 334 NHVQQHQHIDVNRLASVLVNHEMNMIFGKIIAFNPFKTLGDLKIWMASIECYQRYKSNNI 393
              +Q+    +      + N +      KII     +       W+   E   + +++  
Sbjct: 356 TRGRQYVQAALEEEKKRVENQK------KIIQVIQQERFLKKLAWIED-EYKPKCQAHKN 415

Query: 394 GYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQSRLSRRRAPIRL-----WYLLFAGT 453
           GYYDS+K ++ E DFK    +  L   ++ V+     L + + P        W  L   T
Sbjct: 416 GYYDSFKVSNEENDFKANVKRAELAGVFDEVL---GLLKKCQLPDEFEGDIDWIKL--AT 475

Query: 454 NYRRFIEPLDIAEHYRKGLNN-----YESHRPKHYRKLEEWFEEECAP------------ 498
            YRR +EPLDIA ++R   N       +  RP  Y   +  +E                 
Sbjct: 476 RYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHHILKPNGMIAEDVFWN 535

BLAST of Spg010882 vs. ExPASy Swiss-Prot
Match: Q9SU71 (Protein EDS1B OS=Arabidopsis thaliana OX=3702 GN=EDS1B PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 7.5e-07
Identity = 59/221 (26.70%), Postives = 93/221 (42.08%), Query Frame = 0

Query: 309 IECYQ-RYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENV--VEQQSRLSRRRAPI 368
           +E Y+ + +++  GYYDS+K+++ E DFK    +  L   ++ V  + ++ +L       
Sbjct: 397 VEVYKPKCQAHKNGYYDSFKDSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDGFEGS 456

Query: 369 RLWYLLFAGTNYRRFIEPLDIAEHYRKGLNN----YESH-RPKHYRKLEEWFEEECAPHL 428
           R W  L   T YRR IEPLDI+ ++ +  N     Y  H RP  Y+  +  +E +     
Sbjct: 457 RGWINL--ATQYRRLIEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILKPT 516

Query: 429 ------------------TKQSVPPTLTRD-----SCFWAHVEEAILECQKLKMVNCSAR 488
                              +Q +   L        SCFWA VEE               +
Sbjct: 517 GMIAKDVFWSKVNGLNLGLQQDIQEILKNSGSECGSCFWAEVEE------------LKGK 576

Query: 489 DKEVVLAKLTKFEDYVVELMKNYDV-SSEIFLPHSTFMRWW 498
             E V  +    E  +   +K+ +V   EIFL  STF +WW
Sbjct: 577 PYEEVQVRFKTLEGLLEGWIKDGEVDEKEIFLEGSTFRKWW 603

BLAST of Spg010882 vs. ExPASy TrEMBL
Match: A0A6J1F5N9 (senescence-associated carboxylesterase 101 OS=Cucurbita moschata OX=3662 GN=LOC111442342 PE=4 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 1.1e-194
Identity = 358/539 (66.42%), Postives = 406/539 (75.32%), Query Frame = 0

Query: 2   CLAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCS 61
           CLAF+GHY+ERSNC+I+AFVTTPNC+ENPLEEEGG DLVSLSDLKDEFTAFEFL S+ CS
Sbjct: 46  CLAFFGHYNERSNCKILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCS 105

Query: 62  DSAINGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPT 121
           DSAINGAALDLF RFY   EQEI  L SN  TQ IITGH LGG +ASLFTLLLLD +D T
Sbjct: 106 DSAINGAALDLFGRFYPVYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLT 165

Query: 122 KP--PLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTF 181
           K   PLCIT+GSPLLGNE LQ AIS+ S W S FLHVVSN DPLP   LN++ YYPFGTF
Sbjct: 166 KAKRPLCITFGSPLLGNEALQSAISQFSTWSSCFLHVVSNRDPLPTSLLNNKAYYPFGTF 225

Query: 182 LFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSIT 241
           LFCSE GGC+C EDP ST KLLEAT    +  +S  FDYKETI RLI+   LK TT+ IT
Sbjct: 226 LFCSESGGCSCFEDPKSTSKLLEATKANTHLTASSFFDYKETIHRLIDQANLKATTRLIT 285

Query: 242 KNTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPF- 301
           +N+ES   SFIAQ++AIG V +  QQ Q   +DV RLA  L  +EMNMIF K    NPF 
Sbjct: 286 ENSESWPASFIAQLEAIGVVPDQAQQQQQRAVDVRRLAMALQGNEMNMIFEKT---NPFA 345

Query: 302 KTLGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQ 361
           KTL D+KI MA +E Y ++  S +IGYYDSYKN   E D KVAE KK L VYWEN VE+ 
Sbjct: 346 KTLNDVKINMAKLEWYKKKCNSESIGYYDSYKNGRTEPDIKVAEFKKILLVYWENKVEEA 405

Query: 362 SRLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEE 421
            R   +        LLF GTNYRR +EPLDIAEHYRKGL NY++HRPKHYRKLEEW+ E 
Sbjct: 406 ERKPVKEGSPLNVRLLFGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNES 465

Query: 422 CAPHLT---KQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDY 481
             P  +   ++SV   LT DS FWAHVEEA++ CQ L+MV+ SA +KE  LAKL +FE+Y
Sbjct: 466 MTPESSSTQRESVSSILTVDSLFWAHVEEAVVACQVLRMVDSSAEEKEAELAKLKEFEEY 525

Query: 482 VVELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           VVELMKNY VSSEIFLP S+FMRWW+EYD IVGD HDSVLT LMRNEEY EYA G L+I
Sbjct: 526 VVELMKNYGVSSEIFLPQSSFMRWWDEYDGIVGDGHDSVLTMLMRNEEYKEYANGRLDI 581

BLAST of Spg010882 vs. ExPASy TrEMBL
Match: A0A6J1J8J7 (senescence-associated carboxylesterase 101 OS=Cucurbita maxima OX=3661 GN=LOC111482262 PE=4 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 5.7e-191
Identity = 351/538 (65.24%), Postives = 403/538 (74.91%), Query Frame = 0

Query: 2   CLAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCS 61
           CLAF+GHY+ERSNC+I+AFVTTPNC+ENPLEEEGG DLVSLSDLKDEFTAFEFL S+ CS
Sbjct: 46  CLAFFGHYNERSNCKILAFVTTPNCSENPLEEEGGEDLVSLSDLKDEFTAFEFLYSESCS 105

Query: 62  DSAINGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPT 121
           DSAINGAALDLF  FY   EQEI  L SN  TQ IITGH LGG +ASLFTLLLLD +D T
Sbjct: 106 DSAINGAALDLFGPFYPIYEQEISRLKSNPKTQLIITGHGLGGSVASLFTLLLLDSIDLT 165

Query: 122 KP--PLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTF 181
           K   PLCIT+GSPLLGNE LQ AIS+ S W S FLHVVSN DPLP   LN++ YYPFGTF
Sbjct: 166 KAKRPLCITFGSPLLGNEALQSAISQFSTWSSCFLHVVSNRDPLPTSLLNNKAYYPFGTF 225

Query: 182 LFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSIT 241
           LFCSE GGC+C EDP STLKLLEAT    +  +S  FDYKETI RLI+   LK TT+ IT
Sbjct: 226 LFCSESGGCSCFEDPKSTLKLLEATKANTHLTASSFFDYKETIHRLIDQANLKATTRLIT 285

Query: 242 KNTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPFK 301
           +N+ S   SFIAQ++AIG V +  QQ Q   +DV RLA  L ++EMNMIF K    +  K
Sbjct: 286 ENSVSRTASFIAQLEAIGVVPDQAQQQQQRVVDVKRLAVELKDNEMNMIFEK--TNSSAK 345

Query: 302 TLGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQS 361
           TL D+KI MA +E Y ++  S +IGYYDSYKN  +E D KVAE KK L VYWEN VE+  
Sbjct: 346 TLNDVKINMAKLEWYKKKCNSESIGYYDSYKNGRSEPDIKVAEFKKILLVYWENKVEEAE 405

Query: 362 RLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEEC 421
           R   +        LLF GTNYRR +EPLDIAEHYRKGL NY++HRPKHYRKLEEW+ E  
Sbjct: 406 RKPVKEGSPLNVRLLFGGTNYRRMVEPLDIAEHYRKGLKNYKAHRPKHYRKLEEWYNEST 465

Query: 422 APHLT---KQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDYV 481
            P  +   ++SV   LT DS FW HVEEA++ CQ L+ V+ SA +KE  LAKL +FE+YV
Sbjct: 466 TPESSSTQRESVSSILTVDSLFWEHVEEAVVACQVLRTVDSSAEEKEAELAKLKEFEEYV 525

Query: 482 VELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           VELMK+Y VSSEIFLP S+FMRWW+EYD IVGD HDSVLT LMRNEEY EYA G L+I
Sbjct: 526 VELMKSYGVSSEIFLPQSSFMRWWDEYDGIVGDGHDSVLTMLMRNEEYKEYANGRLDI 581

BLAST of Spg010882 vs. ExPASy TrEMBL
Match: A0A6J1DAC5 (senescence-associated carboxylesterase 101 OS=Momordica charantia OX=3673 GN=LOC111018337 PE=4 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 2.3e-184
Identity = 341/537 (63.50%), Postives = 400/537 (74.49%), Query Frame = 0

Query: 4   AFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCSDS 63
           AF+G Y+ERSNC IIAFVT PNC ENPLE E GGDLVS SD KD F AFEFLCSK CSD 
Sbjct: 48  AFFGRYNERSNCEIIAFVTAPNCRENPLEGERGGDLVSFSDFKDVFPAFEFLCSKQCSDF 107

Query: 64  AINGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPTKP 123
           AINGAAL+LF  FY   EQEI+ L SN  TQ IITG ALGG +ASLFTL LLD LD TK 
Sbjct: 108 AINGAALELFWDFYDVYEQEIVRLKSNPKTQLIITGDALGGSVASLFTLFLLDFLDLTKA 167

Query: 124 --PLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTFLF 183
             PLCIT+GSPLLG+E LQ AIS+ S W S FLHVVSNHDPLPR FLNH  YYPFGTFLF
Sbjct: 168 KRPLCITFGSPLLGDEALQSAISQFSTWSSCFLHVVSNHDPLPRMFLNHTAYYPFGTFLF 227

Query: 184 CSEFGGCACSEDPTSTLKLLEATTVQN--NPLSSLLFDYKETIDRLINWVTLKGTTKSIT 243
           CSE  GCACS DP +  KLLEAT V N  +   SLLFDYKET+D LIN   LK  +  IT
Sbjct: 228 CSESDGCACSGDPATVSKLLEATAVPNVTSNSHSLLFDYKETVDHLINQANLKAMSMLIT 287

Query: 244 KNTESLRGSFIAQIDAIGAVKNHVQQHQHIDVNRLASVLVNHEMNMIFGKIIAFNPFKTL 303
           +NTESLRGSF+AQ++AIG V+   QQ Q++DVN+L   ++  E + I  +    +P + L
Sbjct: 288 ENTESLRGSFVAQLEAIGVVQIQAQQQQNVDVNKLVKKMIERENDTIL-ETKTSDPSEAL 347

Query: 304 GDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQSRL 363
             +KI MA +E Y ++ +S++IGYYDSYKN  +  D +VAE KK LRVYWE +VE+  R 
Sbjct: 348 NMVKINMAKLEWYKKKCQSDDIGYYDSYKNQRHPPDIRVAEFKKKLRVYWEKMVEEAERR 407

Query: 364 SRRR-APIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEECA 423
            +R  AP  + + +F GTNYRR +EPLDIAEHYRKGL +Y+SHRPKHY KLEEWFEE  +
Sbjct: 408 PQREGAPFNIRW-MFGGTNYRRMVEPLDIAEHYRKGLRDYKSHRPKHYEKLEEWFEESSS 467

Query: 424 PHLT--KQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDYVVE 483
              T  K+SV   LT DSCFWA+VEEAIL CQ +KMV+CSA ++E  + KL  FEDYVV 
Sbjct: 468 DPSTGQKESVSSILTVDSCFWANVEEAILACQVVKMVDCSAEEEEEAMGKLKDFEDYVVR 527

Query: 484 LMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEE-YVEYAKGWLEI 532
           +MKN  VSSEIFLP S+FMRWWE+YDEIVGDDHDS+L+ LMRNE+ Y +YA G LEI
Sbjct: 528 MMKNSAVSSEIFLPQSSFMRWWEQYDEIVGDDHDSILSVLMRNEDHYEQYANGRLEI 582

BLAST of Spg010882 vs. ExPASy TrEMBL
Match: A0A5D3D1Z0 (Senescence-associated carboxylesterase 101 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00280 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 6.5e-179
Identity = 331/537 (61.64%), Postives = 392/537 (73.00%), Query Frame = 0

Query: 6   YGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDL-KDEFTAFEFLCSKGCSDSA 65
           +G+ +ER NC+I+AFVT PN TENP+E +GG DLV +SD+ KDEFTAFEFL S+ CSDSA
Sbjct: 50  HGYCNERLNCKIVAFVTAPNFTENPIEGDGGEDLVLVSDMVKDEFTAFEFLQSRTCSDSA 109

Query: 66  INGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLD--PTK 125
           ING AL+LF RFY   EQEI  LISN NTQ IITGH LGG +ASLFTLLLLD +D   TK
Sbjct: 110 INGVALELFRRFYPVYEQEISRLISNPNTQLIITGHGLGGSVASLFTLLLLDCIDLTKTK 169

Query: 126 PPLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTFLFC 185
            PLCIT+GSPLLGNE  Q AIS  S W S FLH+VSN DPLPR+ LN++ YYPFGTFLFC
Sbjct: 170 RPLCITFGSPLLGNEAFQNAISHFSTWSSCFLHLVSNQDPLPRKLLNNKAYYPFGTFLFC 229

Query: 186 SEFGGCACSEDPTSTLKLLEATTVQNNPL--SSLLFDYKETIDRLINWVTLKGTTKSITK 245
           S+ GGC+C EDP S LK+LEAT   N+ L  +S  FDYKETIDRLI     K       +
Sbjct: 230 SQSGGCSCFEDPKSILKMLEATKANNDVLLNASAFFDYKETIDRLIKQTNFKANINVKIE 289

Query: 246 NTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPFKT 305
           N ES  GSF+AQ++AIG  +N  QQ Q   +D+NRLA  L ++EMNMI      F   KT
Sbjct: 290 NAESWTGSFLAQLEAIGVAQNQAQQQQQRVVDINRLAKTLKDNEMNMILENTKPF--AKT 349

Query: 306 LGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQSR 365
           L D+KI MA +E Y ++ K +NIGYYDSYKN  +E D +VAE KK L+VYWEN VE+  R
Sbjct: 350 LNDVKINMAKLEWYKKKCKFDNIGYYDSYKNQMSEPDIRVAEFKKILQVYWENKVEEAER 409

Query: 366 LSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEECA 425
              R        LLF GTNYRR +EPLDIAEHYRKGL +Y+SHRPKHY KLE+WF++   
Sbjct: 410 KPLRDGAPFDVKLLFGGTNYRRMVEPLDIAEHYRKGLKDYKSHRPKHYTKLEQWFKDLKT 469

Query: 426 P---HLTKQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDYVV 485
           P    + +++V   LT DS FW HVEEAIL C  ++  +CS  ++E  LAKLT+FEDYV+
Sbjct: 470 PDSSSMQEEAVSSILTVDSLFWVHVEEAILACDLVREGDCSEEEREAELAKLTEFEDYVL 529

Query: 486 ELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           ELM+NY VSSEIFLPHSTFM+WWEEYDEIVGDDHDSVL+ LMRNEEY EY  G L I
Sbjct: 530 ELMRNYAVSSEIFLPHSTFMKWWEEYDEIVGDDHDSVLSGLMRNEEYEEYGNGRLVI 584

BLAST of Spg010882 vs. ExPASy TrEMBL
Match: A0A1S3B7N2 (senescence-associated carboxylesterase 101 OS=Cucumis melo OX=3656 GN=LOC103487057 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 6.5e-179
Identity = 331/537 (61.64%), Postives = 392/537 (73.00%), Query Frame = 0

Query: 6   YGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDL-KDEFTAFEFLCSKGCSDSA 65
           +G+ +ER NC+I+AFVT PN TENP+E +GG DLV +SD+ KDEFTAFEFL S+ CSDSA
Sbjct: 50  HGYCNERLNCKIVAFVTAPNFTENPIEGDGGEDLVLVSDMVKDEFTAFEFLQSRTCSDSA 109

Query: 66  INGAALDLFCRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLD--PTK 125
           ING AL+LF RFY   EQEI  LISN NTQ IITGH LGG +ASLFTLLLLD +D   TK
Sbjct: 110 INGVALELFRRFYPVYEQEISRLISNPNTQLIITGHGLGGSVASLFTLLLLDCIDLTKTK 169

Query: 126 PPLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTFLFC 185
            PLCIT+GSPLLGNE  Q AIS  S W S FLH+VSN DPLPR+ LN++ YYPFGTFLFC
Sbjct: 170 RPLCITFGSPLLGNEAFQNAISHFSTWSSCFLHLVSNQDPLPRKLLNNKAYYPFGTFLFC 229

Query: 186 SEFGGCACSEDPTSTLKLLEATTVQNNPL--SSLLFDYKETIDRLINWVTLKGTTKSITK 245
           S+ GGC+C EDP S LK+LEAT   N+ L  +S  FDYKETIDRLI     K       +
Sbjct: 230 SQSGGCSCFEDPKSILKMLEATKANNDVLLNASAFFDYKETIDRLIKQTNFKANINVKIE 289

Query: 246 NTESLRGSFIAQIDAIGAVKNHVQQHQH--IDVNRLASVLVNHEMNMIFGKIIAFNPFKT 305
           N ES  GSF+AQ++AIG  +N  QQ Q   +D+NRLA  L ++EMNMI      F   KT
Sbjct: 290 NAESWTGSFLAQLEAIGVAQNQAQQQQQRVVDINRLAKTLKDNEMNMILENTKPF--AKT 349

Query: 306 LGDLKIWMASIECY-QRYKSNNIGYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQSR 365
           L D+KI MA +E Y ++ K +NIGYYDSYKN  +E D +VAE KK L+VYWEN VE+  R
Sbjct: 350 LNDVKINMAKLEWYKKKCKFDNIGYYDSYKNQMSEPDIRVAEFKKILQVYWENKVEEAER 409

Query: 366 LSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYESHRPKHYRKLEEWFEEECA 425
              R        LLF GTNYRR +EPLDIAEHYRKGL +Y+SHRPKHY KLE+WF++   
Sbjct: 410 KPLRDGAPFDVKLLFGGTNYRRMVEPLDIAEHYRKGLKDYKSHRPKHYTKLEQWFKDLKT 469

Query: 426 P---HLTKQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARDKEVVLAKLTKFEDYVV 485
           P    + +++V   LT DS FW HVEEAIL C  ++  +CS  ++E  LAKLT+FEDYV+
Sbjct: 470 PDSSSMQEEAVSSILTVDSLFWVHVEEAILACDLVREGDCSEEEREAELAKLTEFEDYVL 529

Query: 486 ELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYAKGWLEI 532
           ELM+NY VSSEIFLPHSTFM+WWEEYDEIVGDDHDSVL+ LMRNEEY EY  G L I
Sbjct: 530 ELMRNYAVSSEIFLPHSTFMKWWEEYDEIVGDDHDSVLSGLMRNEEYEEYGNGRLVI 584

BLAST of Spg010882 vs. TAIR 10
Match: AT5G14930.2 (senescence-associated gene 101 )

HSP 1 Score: 251.1 bits (640), Expect = 1.9e-66
Identity = 190/541 (35.12%), Postives = 269/541 (49.72%), Query Frame = 0

Query: 3   LAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCSD 62
           L F  H +E+    ++ F   P C  +  +        +L  +KD+   F FLCS+    
Sbjct: 55  LEFQIHREEKFT--LVVFSAPPICRSSSSDS-------TLLHVKDKENPFPFLCSENNPS 114

Query: 63  SAINGAALDLF---CRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLD 122
            +++  A +LF        + + E+L  + +     IITG ALGG +ASL+TL LL+ ++
Sbjct: 115 FSLHTPAFNLFTSASTSLTYLKSELLQTLKSEK-PVIITGAALGGSVASLYTLWLLETIE 174

Query: 123 PT-KPPLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGT 182
           PT K PLCIT+GSPL+G+  LQ+ I E S   S FLHVVS    +   F     + PFGT
Sbjct: 175 PTLKRPLCITFGSPLIGDASLQQ-ILENSVRNSCFLHVVSAQTRIKMDF-----FKPFGT 234

Query: 183 FLFCSEFGGCACSEDPTSTLKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSI 242
           FL C +  GC C ED  +  +LL      N    S L DY + ++RL             
Sbjct: 235 FLICFD-SGCVCIEDHVAVTELL------NGVHDSGLVDYSQVLNRL------------- 294

Query: 243 TKNTESLRGSFIAQIDAIGAVKNHVQQHQHIDVNRLASVLVNHEMNMIFGKIIAFNPFKT 302
            ++  SL  S +   D I  ++   +             + N   +M+         FK 
Sbjct: 295 DQSMLSLADSRLIPEDVIKGIEKRAE-------------MKNLRFDMM---------FKK 354

Query: 303 LGDLKIWMASIECY-QRYKSNNIGYYDSYKN----ASNELDFKVA-ELKKFLRVYWENVV 362
           L D+KI MA IE Y ++ K   IGYYD +K      S E D  +    K  L  +W++VV
Sbjct: 355 LNDMKISMAYIEWYKKKCKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVV 414

Query: 363 EQQSRLSRRRAPIRLWYLLFAGTNYRRFIEPLDIAEHYRKGLNNYE-SHRPKHYRKLEEW 422
           E+  R  +  A I     LF+G NYRR IEPLDIAE+Y +G   Y  + R  HY  LE+W
Sbjct: 415 EEVERRPQSDASILKRRFLFSGNNYRRMIEPLDIAEYYLEGRKEYRTTGRSHHYVMLEKW 474

Query: 423 FEEEC----APHLTKQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCSARD--KEVVLAK 482
           F  E          K+ +   LT DSCFWA VE++++   +L       RD  +EV+  K
Sbjct: 475 FGMESILIEKERCKKRDLSDLLTFDSCFWAEVEDSLIVINQLN-TTVGMRDDVREVLTRK 534

Query: 483 LTKFEDYVVELMKNYDVSSEIFLPHSTFMRWWEEYDEIVGDDHDSVLTRLMRNEEYVEYA 527
           L +FE YV E++   +VS EIFL  S+FM+WW+EY +I G  + S LT  M   +Y  Y 
Sbjct: 535 LVEFEGYVWEIITKREVSPEIFLEESSFMKWWKEYKKIKG-FNSSYLTEFMNTRKYESYG 535

BLAST of Spg010882 vs. TAIR 10
Match: AT3G52430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 139.8 bits (351), Expect = 6.3e-33
Identity = 132/490 (26.94%), Postives = 215/490 (43.88%), Query Frame = 0

Query: 65  INGAALDLF--CRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLDPTK 124
           ++ A L LF   +     E E+LG         +ITGH+ GG +A+   L LL    P  
Sbjct: 85  VDAAILKLFLQLKIKEGLELELLG-----KKLVVITGHSTGGALAAFTALWLLSQSSPPS 144

Query: 125 -PPLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGTFLF 184
               CIT+GSPLLGN+ L  +IS  S+    F HVVS HD +PR   ++  ++PFGT+LF
Sbjct: 145 FRVFCITFGSPLLGNQSLSTSISR-SRLAHNFCHVVSIHDLVPRS--SNEQFWPFGTYLF 204

Query: 185 CSEFGGCACSEDPTST---LKLLEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTT--- 244
           CS+ GG  C ++  S      +L  T  QN         Y  T+  +     LK  +   
Sbjct: 205 CSDKGG-VCLDNAGSVRLMFNILNTTATQNTEEHQRYGHYVFTLSHMF----LKSRSFLG 264

Query: 245 KSITKNTESLRGSFIAQIDAIG---------AVKNHVQQHQHI-DVNRLASVLVNHEMNM 304
            SI  N  S +      ++A+G          VK  ++    I     L S  + +E+  
Sbjct: 265 GSIPDN--SYQAGVALAVEALGFSNDDTSGVLVKECIETATRIVRAPILRSAELANELAS 324

Query: 305 IFGKIIAFNPFKTLGDLKIWMASIECYQRYKSNNIGYYDSYKNASNELDFKVAELKKFLR 364
           +    +    +K   D                  +GYYD +K  S + DFKV   +  L 
Sbjct: 325 VLPARLEIQWYKDRCDA-------------SEEQLGYYDFFKRYSLKRDFKVNMSRIRLA 384

Query: 365 VYWENVVEQQSRLSRRRAPI-----RLWYLLFAGTNYRRFIEPLDIAEHYRK-----GLN 424
            +W+ V++    +     P      + W  ++A   Y+   EPLDIA  Y+      G +
Sbjct: 385 KFWDTVIKM---VETNELPFDFHLGKKW--IYASQFYQLLAEPLDIANFYKNRDIKTGGH 444

Query: 425 NYESHRPKHYRKLEEWFEEECAPHLTKQSVPPTLTRDSCFWAHVEEAILECQKLKMVNCS 484
             E +RPK Y  +++W +    P    +S   + T+D+CFWA +E+A     + +  +  
Sbjct: 445 YLEGNRPKRYEVIDKWQKGVKVPEECVRSRYASTTQDTCFWAKLEQAKEWLDEARKESSD 504

Query: 485 ARDKEVVLAKLTKFEDYVVELMKNYDVSSEIFLPHSTFMRW------------WEEYDEI 514
            + + ++  K+  FE Y   L+   +VS ++   +S++  W            +E   E+
Sbjct: 505 PQRRSLLREKIVPFESYANTLVTKKEVSLDVKAKNSSYSVWEANLKEFKCKMGYENEIEM 541

BLAST of Spg010882 vs. TAIR 10
Match: AT5G14930.3 (senescence-associated gene 101 )

HSP 1 Score: 106.3 bits (264), Expect = 7.7e-23
Identity = 73/195 (37.44%), Postives = 104/195 (53.33%), Query Frame = 0

Query: 3   LAFYGHYDERSNCRIIAFVTTPNCTENPLEEEGGGDLVSLSDLKDEFTAFEFLCSKGCSD 62
           L F  H +E+    ++ F   P C  +  +        +L  +KD+   F FLCS+    
Sbjct: 55  LEFQIHREEKFT--LVVFSAPPICRSSSSDS-------TLLHVKDKENPFPFLCSENNPS 114

Query: 63  SAINGAALDLF---CRFYHFCEQEILGLISNRNTQYIITGHALGGWIASLFTLLLLDVLD 122
            +++  A +LF        + + E+L  + +     IITG ALGG +ASL+TL LL+ ++
Sbjct: 115 FSLHTPAFNLFTSASTSLTYLKSELLQTLKSEK-PVIITGAALGGSVASLYTLWLLETIE 174

Query: 123 PT-KPPLCITYGSPLLGNEGLQKAISELSKWPSFFLHVVSNHDPLPRRFLNHRGYYPFGT 182
           PT K PLCIT+GSPL+G+  LQ+ I E S   S FLHVVS    +   F     + PFGT
Sbjct: 175 PTLKRPLCITFGSPLIGDASLQQ-ILENSVRNSCFLHVVSAQTRIKMDF-----FKPFGT 232

Query: 183 FLFCSEFGGCACSED 194
           FL C +  GC C ED
Sbjct: 235 FLICFD-SGCVCIED 232

BLAST of Spg010882 vs. TAIR 10
Match: AT3G48090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 8.8e-11
Identity = 110/512 (21.48%), Postives = 181/512 (35.35%), Query Frame = 0

Query: 94  QYIITGHALGGWIASLFTLLLLDVLDPTKP-----PLCITYGSPLLGNEGLQKAISELSK 153
           Q + TGH+ GG  A L T+  L+      P     P C+T+G+PL+G+     A+    K
Sbjct: 116 QIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGR-EK 175

Query: 154 WPSFFLHVVSNHDPLPR------------------------------------------- 213
           W  FF++ VS  D +PR                                           
Sbjct: 176 WSRFFVNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMR 235

Query: 214 -------------------------RFLNHRGYYPFGTFLFCSEFGGCACSEDPTSTLKL 273
                                     FL    Y P GTF+F +E    A + +  + L++
Sbjct: 236 DTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVN-NSDAILQM 295

Query: 274 LEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSITKNTESLRGSFIAQIDAIGAVK 333
           L  T+  ++     L  ++   D       ++   K +  + +    S  + ++ +G V 
Sbjct: 296 LFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDG-ENSIESTLNDLG-VS 355

Query: 334 NHVQQHQHIDVNRLASVLVNHEMNMIFGKIIAFNPFKTLGDLKIWMASIECYQRYKSNNI 393
              +Q+    +      + N +      KII     +       W+   E   + +++  
Sbjct: 356 TRGRQYVQAALEEEKKRVENQK------KIIQVIEQERFLKKLAWIED-EYKPKCQAHKN 415

Query: 394 GYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQSRLSRRRAPIRL-----WYLLFAGT 453
           GYYDS+K ++ E DFK    +  L   ++ V+     + + + P        W  L   T
Sbjct: 416 GYYDSFKVSNEENDFKANVKRAELAGVFDEVL---GLMKKCQLPDEFEGDIDWIKL--AT 475

Query: 454 NYRRFIEPLDIAEHYRKGLNN-----YESHRPKHYRKLEEWFEEECAP------------ 498
            YRR +EPLDIA ++R   N       +  RP  Y   +  +E                 
Sbjct: 476 RYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWN 535

BLAST of Spg010882 vs. TAIR 10
Match: AT3G48090.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 8.8e-11
Identity = 110/512 (21.48%), Postives = 181/512 (35.35%), Query Frame = 0

Query: 94  QYIITGHALGGWIASLFTLLLLDVLDPTKP-----PLCITYGSPLLGNEGLQKAISELSK 153
           Q + TGH+ GG  A L T+  L+      P     P C+T+G+PL+G+     A+    K
Sbjct: 8   QIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGR-EK 67

Query: 154 WPSFFLHVVSNHDPLPR------------------------------------------- 213
           W  FF++ VS  D +PR                                           
Sbjct: 68  WSRFFVNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMR 127

Query: 214 -------------------------RFLNHRGYYPFGTFLFCSEFGGCACSEDPTSTLKL 273
                                     FL    Y P GTF+F +E    A + +  + L++
Sbjct: 128 DTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVN-NSDAILQM 187

Query: 274 LEATTVQNNPLSSLLFDYKETIDRLINWVTLKGTTKSITKNTESLRGSFIAQIDAIGAVK 333
           L  T+  ++     L  ++   D       ++   K +  + +    S  + ++ +G V 
Sbjct: 188 LFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDG-ENSIESTLNDLG-VS 247

Query: 334 NHVQQHQHIDVNRLASVLVNHEMNMIFGKIIAFNPFKTLGDLKIWMASIECYQRYKSNNI 393
              +Q+    +      + N +      KII     +       W+   E   + +++  
Sbjct: 248 TRGRQYVQAALEEEKKRVENQK------KIIQVIEQERFLKKLAWIED-EYKPKCQAHKN 307

Query: 394 GYYDSYKNASNELDFKVAELKKFLRVYWENVVEQQSRLSRRRAPIRL-----WYLLFAGT 453
           GYYDS+K ++ E DFK    +  L   ++ V+     + + + P        W  L   T
Sbjct: 308 GYYDSFKVSNEENDFKANVKRAELAGVFDEVL---GLMKKCQLPDEFEGDIDWIKL--AT 367

Query: 454 NYRRFIEPLDIAEHYRKGLNN-----YESHRPKHYRKLEEWFEEECAP------------ 498
            YRR +EPLDIA ++R   N       +  RP  Y   +  +E                 
Sbjct: 368 RYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWN 427