Spg010805 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg010805 vs. NCBI nr
Match: XP_008459427.1 (PREDICTED: probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucumis melo] >ADN34284.1 beta-glucan-binding protein [Cucumis melo subsp. melo] >KAA0039392.1 putative endo-1,3(4)-beta-glucanase [Cucumis melo var. makuwa] >TYK00580.1 putative endo-1,3(4)-beta-glucanase [Cucumis melo var. makuwa])

HSP 1 Score: 1298.1 bits (3358), Expect = 0.0e+00
Identity = 632/689 (91.73%), Postives = 649/689 (94.19%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP QPFWQS   P  A  FPQ QSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 57  MSSPSQPFWQS--PPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 116

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLSTVSISYPSMFSNSAFG+QVFNADWT+SENPSSVSQKPHIISSFSD
Sbjct: 117 QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSENPSSVSQKPHIISSFSD 176

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSLTLDIPSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTVTLNNN
Sbjct: 177 LSLTLDIPSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNN 236

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIY+SSPINLTHSLSQITSGGF GIVRIAVLP+PHCET+LDRF SCYPVSGE NFRN
Sbjct: 237 QTWLIYASSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRN 296

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           P  LEYKWEKKGWGNLLMLAHPLHLRLL+ + S AVVLD FKYKSIDGDLVGVVGGSWVL
Sbjct: 297 PFSLEYKWEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVL 356

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPV VTWHSING+GEEF SEIISALVKDVEGLKSSPITTT SYFYGKSIARAARLALI
Sbjct: 357 KPDPVYVTWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALI 416

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVNYL+VIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG++TQQGS DSG DFGFGV
Sbjct: 417 AEEVNYLQVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGV 476

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNSMFPRLRCFDLY
Sbjct: 477 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLY 536

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQESTSEAVN YYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 537 KLHSWASGLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 596

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQIKEGDNLY+ DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITE+L
Sbjct: 597 WWQIKEGDNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEIL 656

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA F KELVDWTLPSLGREGVGEGWKGFAYALQGI+DKDGALE IRNLTGFDDGNSLS
Sbjct: 657 FSDADFVKELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLS 716

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG +E EG G GWKHWWFSH
Sbjct: 717 NLLWWIHSRGGGDEVEGCGGGWKHWWFSH 743

BLAST of Spg010805 vs. NCBI nr
Match: XP_038890545.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Benincasa hispida])

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 631/691 (91.32%), Postives = 651/691 (94.21%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP QPFWQS   P  A  FPQ QSTVLPDPS FFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 51  MSSPTQPFWQS--PPPVAALFPQIQSTVLPDPSKFFAPHLLSSPLPTNSFFQNFTVKNGD 110

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEY HPYLIKSSLSTVSISYPSMF NSAFGYQVFNADWT+SENPSSVSQKPHIISSFSD
Sbjct: 111 QPEYFHPYLIKSSLSTVSISYPSMFLNSAFGYQVFNADWTVSENPSSVSQKPHIISSFSD 170

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSLTLDIPSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTV LNNN
Sbjct: 171 LSLTLDIPSGNLRFFLVRGSPFMTFEVFNNTPISISTIHAILSFSSNSSLTKFTVALNNN 230

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLP+PHCET+LDRF SCYPVSGE NFRN
Sbjct: 231 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRN 290

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           P  LEYKWEK GWGNLLMLAHPLHLRLLS  DS AVVLD FKYKSIDGDLVGVVGGSWVL
Sbjct: 291 PFSLEYKWEKNGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGVVGGSWVL 350

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPVSVTWHSING+GEEF+SEII+ALVKDVEGLKSSPITTT SYFYGKSIARAARLALI
Sbjct: 351 KPDPVSVTWHSINGVGEEFQSEIINALVKDVEGLKSSPITTTSSYFYGKSIARAARLALI 410

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVN+L+VIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGG++TQQGS+DSG DFGFGV
Sbjct: 411 AEEVNFLQVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGLITQQGSNDSGADFGFGV 470

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKFK+ AYSLMADFMNLSRRSNSMFPRLRCFDLY
Sbjct: 471 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKTHAYSLMADFMNLSRRSNSMFPRLRCFDLY 530

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQESTSEAVNAYYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 531 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 590

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQIKEGDNLYD DFARENKVVGVLWSNKRDSGLWFAP DWRECRLGIQVLPLLPITE+L
Sbjct: 591 WWQIKEGDNLYDVDFARENKVVGVLWSNKRDSGLWFAPADWRECRLGIQVLPLLPITEIL 650

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSD GF KELVDWTLPSLGREGVGEGWKGFAYALQG++DKDGA+E I+NLTGFDDGNSLS
Sbjct: 651 FSDPGFVKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGAVERIQNLTGFDDGNSLS 710

Query: 661 NLLWWIHSR--GGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSR  GG +EGEG G GWKHWWFSH
Sbjct: 711 NLLWWIHSRGGGGGDEGEGHGGGWKHWWFSH 739

BLAST of Spg010805 vs. NCBI nr
Match: XP_004141490.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucumis sativus] >KAE8648959.1 hypothetical protein Csa_007756 [Cucumis sativus])

HSP 1 Score: 1288.5 bits (3333), Expect = 0.0e+00
Identity = 624/689 (90.57%), Postives = 647/689 (93.90%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP QPFWQS   P  A  FPQ +S+VLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 56  MSSPSQPFWQS--PPPVAALFPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 115

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLSTVS+SYPSM SNSAFGYQVFNADWT+SENP SVSQKPHIISSFSD
Sbjct: 116 QPEYIHPYLIKSSLSTVSVSYPSMLSNSAFGYQVFNADWTVSENPPSVSQKPHIISSFSD 175

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSLTLDIPSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTVTLNNN
Sbjct: 176 LSLTLDIPSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNN 235

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIY+SSPINLTHSLSQITSGGF+GIVRIAVLP+PHCET+LDRF SCYPVSGE NFRN
Sbjct: 236 QTWLIYASSPINLTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRN 295

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           P  LEYKWEKKGWGNLLMLAHPLHLRLLS + S+AVVLD FKYKSIDGDLVGVVGGSW L
Sbjct: 296 PFSLEYKWEKKGWGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWAL 355

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPV +TWHSING+GEEF SEIISALVKDVEGLKSSPITTT SYFYGKSIARAARLALI
Sbjct: 356 KPDPVYLTWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALI 415

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVNYL+VIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG++TQQGS DSG DFGFGV
Sbjct: 416 AEEVNYLQVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGV 475

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNSMFPRLRCFDLY
Sbjct: 476 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLY 535

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQESTSEAVN YYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 536 KLHSWASGLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 595

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQIKEGDNLY+ DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+L
Sbjct: 596 WWQIKEGDNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEIL 655

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA F KELVDWTLPSLGREGVGEGWKGFAYALQG++DKDGALE IRNLTGFDDGNSLS
Sbjct: 656 FSDADFVKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLS 715

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG +E E  G GWKHWWFSH
Sbjct: 716 NLLWWIHSRGGGDEVEACGGGWKHWWFSH 742

BLAST of Spg010805 vs. NCBI nr
Match: KAG7033239.1 (hypothetical protein SDJN02_07293, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1276.9 bits (3303), Expect = 0.0e+00
Identity = 621/689 (90.13%), Postives = 650/689 (94.34%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP Q F QS   P    PFPQ QSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 48  MSSPSQSFRQS--LPPAVAPFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 107

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLS VSISYPSMFSN+AFGYQVFNADWT+SENP+SVSQKPHIISSFSD
Sbjct: 108 QPEYIHPYLIKSSLSAVSISYPSMFSNTAFGYQVFNADWTVSENPASVSQKPHIISSFSD 167

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSL LD PSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTVTLNNN
Sbjct: 168 LSLILDTPSGNLRFFLVRGSPFITFEVFNNTPISISTIHAILSFSSNSSQTKFTVTLNNN 227

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLP+PH E++LDRF SCYPVSGE NFRN
Sbjct: 228 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPNPHLESILDRFCSCYPVSGEVNFRN 287

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           PS LEYKWEK GWGNLLMLAHPLHLRLLS++DS A+VLD FKYKSIDGDLVGVVGGSWVL
Sbjct: 288 PSSLEYKWEKNGWGNLLMLAHPLHLRLLSSDDS-AIVLDDFKYKSIDGDLVGVVGGSWVL 347

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPVS+TWHSINGIGEEF+SEIISALVKDVEGLKSSPITTT SYFYGK+IARAARLALI
Sbjct: 348 KPDPVSITWHSINGIGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKAIARAARLALI 407

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVN+L+VIPEVRKFLK AIEPWLRGTFNGNGFLYD KWGG++TQQG+SDSG DFGFGV
Sbjct: 408 AEEVNFLQVIPEVRKFLKDAIEPWLRGTFNGNGFLYDSKWGGLVTQQGASDSGADFGFGV 467

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKF+ QAYSLMADFMNLSRRS+S FPRLRCFDLY
Sbjct: 468 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRSSSKFPRLRCFDLY 527

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQES+SEAVNAYYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 528 KLHSWASGLTEFADGRNQESSSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 587

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQI+ G+NLY+TDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQ+LPLLPITE L
Sbjct: 588 WWQIRGGENLYETDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQLLPLLPITEAL 647

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA FAKELVDWTLPSL REGVGEGWKGFAYALQGI+DKDGA++NIRNL GFDDGNSLS
Sbjct: 648 FSDAAFAKELVDWTLPSLRREGVGEGWKGFAYALQGIYDKDGAVQNIRNLKGFDDGNSLS 707

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG EEGEGFG GWKHWWFSH
Sbjct: 708 NLLWWIHSRGGGEEGEGFGGGWKHWWFSH 733

BLAST of Spg010805 vs. NCBI nr
Match: KAG6602560.1 (hypothetical protein SDJN03_07793, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1274.2 bits (3296), Expect = 0.0e+00
Identity = 619/689 (89.84%), Postives = 649/689 (94.19%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP Q F QS   P    PFPQ QSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 48  MSSPSQSFRQS--LPPAVAPFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 107

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLS VSISYPSMFSN+AFGYQVFNADWT+SENP+SVSQKPHIISSFSD
Sbjct: 108 QPEYIHPYLIKSSLSAVSISYPSMFSNTAFGYQVFNADWTVSENPASVSQKPHIISSFSD 167

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSL LD PSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTVTLNNN
Sbjct: 168 LSLILDTPSGNLRFFLVRGSPFITFEVFNNTPISISTIHAILSFSSNSSQTKFTVTLNNN 227

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLP+PH E++LDRF SCYPVSGE NFRN
Sbjct: 228 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPNPHLESILDRFCSCYPVSGEVNFRN 287

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           PS LEYKWEK GWGNLLMLAHPLHLRLLS++DS A+VLD FKYKSIDGDLVGVVGGSWVL
Sbjct: 288 PSSLEYKWEKNGWGNLLMLAHPLHLRLLSSDDS-AIVLDDFKYKSIDGDLVGVVGGSWVL 347

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPVS+TWHSINGIGEEF+SEIISALVKDVEGLKSSPITT  SYFYGK+IARAARLALI
Sbjct: 348 KPDPVSITWHSINGIGEEFQSEIISALVKDVEGLKSSPITTASSYFYGKAIARAARLALI 407

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVN+L+VIPEVRKFLK AIEPWLRGTFNGNGFLYD KWGG++TQQG+SDSG DFGFGV
Sbjct: 408 AEEVNFLQVIPEVRKFLKDAIEPWLRGTFNGNGFLYDSKWGGLVTQQGASDSGADFGFGV 467

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYH+GYFLYAIAVLVKIDPAWGRKF+ QAYSLMADFMNLSRRS+S FPRLRCFDLY
Sbjct: 468 YNDHHYHLGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRSSSKFPRLRCFDLY 527

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQES+SEAVNAYYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 528 KLHSWASGLTEFADGRNQESSSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 587

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQI+ G+NLY+TDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQ+LPLLPITE L
Sbjct: 588 WWQIRGGENLYETDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQLLPLLPITEAL 647

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA FAKELVDWTLPSL REGVGEGWKGFAYALQGI+DKDGA++NIRNL GFDDGNSLS
Sbjct: 648 FSDAAFAKELVDWTLPSLRREGVGEGWKGFAYALQGIYDKDGAVQNIRNLKGFDDGNSLS 707

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG EEGEGFG GWKHWWFSH
Sbjct: 708 NLLWWIHSRGGGEEGEGFGGGWKHWWFSH 733

BLAST of Spg010805 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 3.3e-39
Identity = 163/584 (27.91%), Postives = 258/584 (44.18%), Query Frame = 0

Query: 129 SGNLRFFLVRGSPFLTFEVFNNALISISTIHAIL------SFSSNSSSTKFTVTLNNNQT 188
           S ++ F LV+G  F+T  ++NN   +I +  A+L      + S      K+ +TL +++ 
Sbjct: 335 SESITFPLVQGMGFIT-AIYNNLQPAIQS--AVLFRKVEPAGSPQGGIFKYKITLEDDKN 394

Query: 189 WLIY----SSSPINLTHSLSQITSG--GFSGIVRIAVLPD-PHCETVLDRFYSCY----P 248
           WL+Y    + +   L    +++ SG  GF G++++A  P     E + D+    Y     
Sbjct: 395 WLLYVIPENGADPKLKLEGNKLISGPTGFKGVIQVAKNPSAEEGEGIYDKSAGSYATDIK 454

Query: 249 VSGEANFRNPSCLEYKWEKKGWG-NLLMLAHPLHLRLLSNEDSAAVVLDGFKYK-SIDGD 308
           +SG          ++ +EK G G  L+M A P H+   S +D+        K   +  G 
Sbjct: 455 ISGSVGTDGTGTYKFSFEKAGKGAPLVMYALPHHVE--SFDDATKNTKKNMKLSTTTKGM 514

Query: 309 LVGVVGGSWVLNPD--PVSVTW--HSINGIGEEFRSEIISALVKDVEGLKSS-----PIT 368
               VG SW +     P+S+ +         +   SE     +K V G + S        
Sbjct: 515 ATACVGDSWTMVEGNLPLSMDFAPWKPGSSSQVTLSEGAKNAIKAVAGNELSQDMELQTN 574

Query: 369 TTESYFYGKSIAR-AARLALIAEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGK 428
               YF GK + + A  +  + E V            LK + + ++         +YD  
Sbjct: 575 LNSMYFSGKGLNKFAGAIYTVQELVGDKAAASGPLNSLKESFKRFVDNK-QQIPLVYDNV 634

Query: 429 WGGILTQ--QGSSDSGKDFGFGVYNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLM 488
           W G+++       D+G DFG  +YNDHH+H GYF+   A+L K+DPAW    K+    L+
Sbjct: 635 WKGVVSSGTYEKGDTGLDFGNTLYNDHHFHYGYFILTAAILGKLDPAWLDANKAYVNMLV 694

Query: 489 ADFMNLSRRSNSMFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLA 548
            D  N S  ++  FP  R FD Y  HSWA GL E +DG++QESTSE     Y+  + G  
Sbjct: 695 RDSGN-SVDNDEHFPFSRAFDWYHGHSWAKGLFESSDGKDQESTSEDTMYAYAIKMWGKT 754

Query: 549 YGDTHLASIGSTLTAFEIKAAQTWWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFA 608
            GD  + + G+ +     +    ++ +K  DN+        NKV G+L+ NK D   +F 
Sbjct: 755 SGDKSMEARGNLMLGILARTLNNYFLMK-NDNVNQPKNFIGNKVTGILFENKIDHTTYFG 814

Query: 609 PPDWRECRLGIQVLPLLPITEVLFSDAGFAKELVDWTL-----PSLGREGVGEGWKGFAY 668
                E   GI +LPLLP        A F KE  +W        +   E V  GWKG  Y
Sbjct: 815 AN--LEYIQGIHMLPLLP-NSAYTRSAEFVKE--EWEAMFASGAAAPAEKVTGGWKGVLY 874

Query: 669 ALQGIFDKDGALENIR----NLTGFDDGNSLSNLLWWIHSRGGE 673
           A   I D + + +       +L+  D G   ++ +W++    GE
Sbjct: 875 ANLAIIDPEASWKYFAQPSLDLSSIDGG---ASRIWYLAYAAGE 902

BLAST of Spg010805 vs. ExPASy Swiss-Prot
Match: P53753 (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 3.4e-36
Identity = 186/726 (25.62%), Postives = 309/726 (42.56%), Query Frame = 0

Query: 16   ATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI---KS 75
            A +T  P       P+P +         P+ TN F+ N  V + + P +++PY +    S
Sbjct: 405  AVSTDEPPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTS 464

Query: 76   SLSTVSISYPSMFSNSAFGYQVF-NADWTISENPSSVSQKPHIISSFSDLSLTLDI---- 135
            S    ++ + ++   S  GY    NA++ +  NP  ++      S+F D S+T+ +    
Sbjct: 465  SSYGFAVQHTTVDQYSYGGYDSSGNAEYLV--NPLGIAHVVFSASNF-DSSMTMQVDEMT 524

Query: 136  -------------PSGNLRFFLVRGSPFLT--FEVFNNALISIST-IHAILSFSSNSSS- 195
                          S  L   LV+G  F T  +    NA I  S   + I+S SS++ + 
Sbjct: 525  LSSTRVVLSESNDSSNYLEIPLVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQ 584

Query: 196  --TKFTVTLNNNQTWLIYSSSPINLTHSLSQI---------TSGGFSG-IVRIAVLPDPH 255
               K+ +TL N  TWL Y   P +LT +   +          S    G I+++AV P   
Sbjct: 585  GILKYRITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAPS-- 644

Query: 256  CETVLDRFYSCYPVSGEANFR-------NPSCLEYKWEKKG---WGNLLMLAHPLHLRLL 315
             ET  + FY         NF+       + +  E+ +  +G    G+ ++ A P H    
Sbjct: 645  -ETDYEVFYDQAAGMYVTNFKLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHHESSF 704

Query: 316  SNEDSAAVVLDGFKYKSIDGDLVGVVGG--------SWVLNPDPVSVTWHSINGIG-EEF 375
            S+     ++ D +    +     GV+ G        S  LN     + W S  G    E+
Sbjct: 705  SD-----IMQDYYTGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEY 764

Query: 376  RSEIISALVK----DVEGLKSSPITTTESYFYGKSIARAARLALIAEEVNYLEV-IPEVR 435
              E +  L +    +++   S  I+   +Y+ GK I + + + L   E+   E       
Sbjct: 765  SKEQLQLLAEVANSELQVSISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTL 824

Query: 436  KFLKGAIEPWLRGTFNGNGFLYDGKWGGILT--QQGSSDSGKDFGFGVYNDHHYHIGYFL 495
            + +K A +  L+        +YD K+ G+++    GS+ +  DFG   YNDHH+H GY +
Sbjct: 825  ENIKSAFDILLQNE-QTYPLIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYII 884

Query: 496  YAIAVL----VKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASG 555
            +A AV+     K++  W    K    SL+ D  N S + +  F + R FD +  HSWA+G
Sbjct: 885  HAAAVIGYVDSKLNGTWAADNKDWVNSLVRDVANPSEK-DEYFAQSRMFDWFNGHSWAAG 944

Query: 556  LTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQTWWQIKEGD 615
            L E  +G+N+ES+SE  N  Y+  L G   GD  +   G  + +  +K A   +   + D
Sbjct: 945  LYENGNGKNEESSSEDYNFAYAMKLWGATIGDQSMELRGDLMISI-MKDAMNDYFYYQND 1004

Query: 616  NLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDAGFAK 672
            N  + +    NKV G+L+ N  D   +F      E   GI +LP+ P++  + S+    +
Sbjct: 1005 NTVEPEEIIGNKVSGILFDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSETFVEE 1064

BLAST of Spg010805 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 5.9e-36
Identity = 182/683 (26.65%), Postives = 288/683 (42.17%), Query Frame = 0

Query: 41  LSSPLPTNSFFQNFTVKNGDQPEYIHP--YLIKSSLSTVSISYPSMFSNSAFGYQVFNAD 100
           LSSP+ TN FF N  + +     Y  P  Y  +SS +   I       N     +V + D
Sbjct: 78  LSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAHTDDNQ----RVMDTD 137

Query: 101 WTISE---NPSSV-----------SQKPHIISSFSDLS--LTLDIPSGNLRFFLVRGSPF 160
            TI      P  +           S    I+     +S   T    S +++  L  G   
Sbjct: 138 DTIPSYYYEPIGICSLGFGASGITSNTDPIVDEIDQMSARFTFSWDSSSMQLTLTEGMA- 197

Query: 161 LTFEVFNNALISIST----IHAILSFSSNSSSTKFTVTLNNNQTWLIY-SSSPINLTHSL 220
           +T  V+ NA+  I +    I+  +     S+  K+ VT+++N  WLIY     + LT S 
Sbjct: 198 VTTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDSLTLTEST 257

Query: 221 SQITSGG--FSGIVRIAVLP--DPHCETVLDRFYSCYPVS-GEANFRNPSCLEYKWEKKG 280
           SQ+  G   F+G ++IA +P  D   E + D +   Y      + +   +   Y ++   
Sbjct: 258 SQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVYITGISISGYVEDAVGYYSFDFTT 317

Query: 281 WGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSI------DGDLVGVVGGSWVLN---PD 340
            G+  +   PL   L    D+A   + G K  SI       GD+    G S       P 
Sbjct: 318 AGDTSV--EPLFFLLPHQVDTA---VSGTKVTSIVLASLVSGDMNAAAGNSITFAEAIPQ 377

Query: 341 PVSVTWHSINGIGEEFRSEIISALVKDVEGLK-----SSPITTTESYFYGKSIARAARLA 400
            +     S  G G+   SE    ++ +V G +     S+       Y+ GK +A+ A L 
Sbjct: 378 DIGFLPWSPTG-GQIGYSEEALEIIAEVAGTELGEDFSANSNLNSMYYSGKVLAKYAMLC 437

Query: 401 LIAEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGF--LYDGKWGGILTQQG-SSDSGKD 460
           +   ++   E   E  + ++     + R   N   +   YD  W G+++  G S DS  D
Sbjct: 438 VTINDILGDETSSE--QCIQKLEAAFARFVDNQQIYPLTYDNTWKGVVSVAGLSGDSLAD 497

Query: 461 FGFGVYNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAY--SLMADFMNLSRRSNSMFPR 520
           FG   YNDHH+H GYF++  AV+  IDP W     ++ +   L+ D  N S  ++  FP+
Sbjct: 498 FGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNKEWVNFLVRDVANPS-SNDPYFPK 557

Query: 521 LRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAF 580
            R  D+Y  H WASGL E  DG+++ESTSE  N ++   L G   GD+ +    + +   
Sbjct: 558 HRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFFGMKLWGQVIGDSDMEDRANIILGI 617

Query: 581 EIKAAQTWWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPL 640
           E  A   +    +G N+  T   + N V G+ + NK     +F      E   GI +LP+
Sbjct: 618 ERNALNKYMLYADG-NVQPTSM-QPNYVAGITFMNKITHTTYFGTN--IEYIQGIHMLPI 677

Query: 641 LPITEVLFSDAGFAKELVDW-TLPSLGREGVGEGWKGFAYALQGIFDKDGALENIR---- 672
            PI+  +    G +  L +W  L +   + V  GW+   YA   I + + + E       
Sbjct: 678 TPISAFI---RGPSFVLAEWNALLASVIDYVDSGWRSLLYANLAIAEPEESYEYFSSSDF 736

BLAST of Spg010805 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 7.7e-36
Identity = 167/684 (24.42%), Postives = 284/684 (41.52%), Query Frame = 0

Query: 44  PLPTNSFFQNFTVKNGDQPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISE 103
           PL TN F+ N  + +  QP + HPY I  S        P +F  +A         +  + 
Sbjct: 110 PLETNKFYTNMLLDDNTQPIWTHPYSIWFSRD------PELFGLAANHTLASQRVFDTTT 169

Query: 104 NPSSVSQKPHIISSF----------------------SDLSLTLDIPSGN-LRFFLVRGS 163
           NP      P  I SF                        + L + + S   + F LV+G 
Sbjct: 170 NPPRFYFNPTNIKSFVFKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGM 229

Query: 164 PFLT--FEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNNQTWLIYSSSP-------- 223
            F+T  +      L S     ++   S N    K+ + L NN+ W++Y +SP        
Sbjct: 230 GFVTAIYHDLGFELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDF 289

Query: 224 -INLTHSLSQITSGGFSGIV-RIAVLPDPHCETVLDRFYSCYPV----SGEANFRNPSCL 283
            I+L  S + I+S   +G++ +++    P     +D    CYPV    SG+    + +  
Sbjct: 290 QISLLDSNTIISSHKINGLICQLSADSVPS----IDMAAGCYPVYCDLSGQTVDEHFTNY 349

Query: 284 EYKWEKKGW---GNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSW--- 343
            + +   G+   G  LM A P H    + E     +       ++ G + G +  S+   
Sbjct: 350 RFNYTVAGYSQSGTTLMYALPHHKAAFTPEMQEREIASSLD-STVKGLMTGYLTNSFDMQ 409

Query: 344 -----VLNPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTES-YFYGKSIA 403
                 L  +PV+++ +      +E  S+I  A V++V+       +  +S YF GK +A
Sbjct: 410 VQVPQELGFEPVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKILA 469

Query: 404 RAARLALIAEEVNYLE-VIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSD 463
           + A +  +   + + E +  E+   L  A+E ++          YD  W GI++   S  
Sbjct: 470 KYAWILYVTHYILHDENLTKELLSKLTIAMERFISNQ-QVLPLNYDVSWKGIIS---SGS 529

Query: 464 SGKDFGFGVYNDHHYHIGYFLYAIAVLVKIDP--------AWGRKFKSQAYSLMADFMNL 523
           S +DFG   YNDHH+H  Y +   A++  +D         +W    +     L+ D+  +
Sbjct: 530 SSQDFGNSYYNDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSGV 589

Query: 524 SRRSNSMFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHL 583
               +  FP+ R FD +  HSWA GL    DG+++ESTSE VN+ Y+  L GL  G++ L
Sbjct: 590 D-NDDPYFPQFRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSKL 649

Query: 584 ASIGSTLTAFEIKAAQTWWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFA-PPDWR 643
             I +          Q+++  +  + +   +F   NKV G+L+ NK D   +F   P + 
Sbjct: 650 TDIANLQLGIMRNVFQSYFLYESNNTVQPKEFI-GNKVSGILFENKIDHATYFGMEPQY- 709

Query: 644 ECRLGIQVLPLLPITEV--LFSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDK 661
                I ++  +PIT          F KE  +  +  +  + V +GWKG       + D 
Sbjct: 710 -----IHMIHAIPITSASSWVRTPNFVKEEWEEKMQPI-IDQVNDGWKGIIMLNMALLDP 769

BLAST of Spg010805 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 6.7e-32
Identity = 174/688 (25.29%), Postives = 290/688 (42.15%), Query Frame = 0

Query: 2   SSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQ 61
           + P+ P + S A P          S  L   SN F       P+ TN F+ N  +     
Sbjct: 9   TGPINPVFPSRAHP----------SPPLGLTSNLF-------PIQTNKFYGNLYIGTRHN 68

Query: 62  PEYIHPYLIK----SSLSTVSISY----------------PSMFSNSAFGYQ-VFNADWT 121
           P + HPY +     SS   ++IS+                   + N A  Y  + +A   
Sbjct: 69  PSWSHPYSVTWLNGSSYYGLAISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREF 128

Query: 122 ISENPSSVSQKPHIISSFSDLSLTLDIPSGNLRFFLVRGSPFLTFEVFN-NALISISTIH 181
            S N  S+ Q  H  S  + LS T    SG +   +V G  F++    N   + + S + 
Sbjct: 129 ASGNLLSLDQSRH-FSIQATLSATTS-GSGTIILPIVAGMGFVSGYYTNLTPVFNSSILF 188

Query: 182 AILSFSSNSSSTKFTVTLNNNQTWLIY-----SSSPINLT-HSLSQI-TSGGFSGIVRIA 241
           + ++  + S   K+ + L + + W +Y     SSS  NLT  S SQ+ TS  F+G+++I 
Sbjct: 189 SSITKINFSRGYKYRIQLTDGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQIC 248

Query: 242 VLP-----DPHCETVLDRFYSCYPVSGEANFRNPSCLEYKWEK---KGWGNL--LMLAHP 301
            +P     + + +T+ D     Y  S   + +        W +    G+ NL  LM A P
Sbjct: 249 KVPNESVNNSYPDTIYDASAGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPLMFALP 308

Query: 302 LHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL----NPDPVS---VTWH-SING 361
            H++   ++  A     G    ++ G +      +W L     P  V    + W+   N 
Sbjct: 309 HHMQSFGSDTQAYKTGLGLASTTM-GIMFAYATKTWHLIEKNLPTQVGFLPIPWNGGSNT 368

Query: 362 IGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALIAEEVNYLEVIPEVR 421
                 + I +A   D+     +       Y  GK +A  A++ L+A  +  L       
Sbjct: 369 YSPTALAAIRAACATDINFDVVNASNLDSMYTSGKIVAMYAQVCLVASRI--LGDSTLTN 428

Query: 422 KFLKGAIEPWLRGTFNGNGF--LYDGKWGGILTQQGSSDSGKDFGFGVYNDHHYHIGYFL 481
             L    +   R T N   +  +YD  + GI++  G S    D+G   YNDHH+H GY +
Sbjct: 429 TGLTKLKQAMARFTTNTQMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHI 488

Query: 482 YAIAVLVKIDPAWGRKFKSQ-AYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASGLTE 541
           YA AV+  +DP+W      +   +L+ D  N S  S++ F   R FD +  HSWA+G+ E
Sbjct: 489 YACAVIGLLDPSWLVNDNIRYVNALLRDSANPS-ESDTYFAMFRNFDWFVGHSWATGIFE 548

Query: 542 FADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQTWWQIKEGDNLY 601
             DG+++ESTSE  N  Y+  L G+   DT L +  + + A    +  T+  +    ++ 
Sbjct: 549 SGDGKDEESTSEDFNFLYATKLWGMVRNDTVLINRANLMLAVLKNSLNTYIYMTPTTSVQ 608

Query: 602 DTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDAGFAKELV 640
            +     N V G+ + NK D   +F+  ++  C+ GI ++P  PI+  L S +   ++  
Sbjct: 609 PSQIL-GNYVTGITFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSPSYVQQDWN 668

BLAST of Spg010805 vs. ExPASy TrEMBL
Match: A0A5A7T7D7 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001960 PE=3 SV=1)

HSP 1 Score: 1298.1 bits (3358), Expect = 0.0e+00
Identity = 632/689 (91.73%), Postives = 649/689 (94.19%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP QPFWQS   P  A  FPQ QSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 57  MSSPSQPFWQS--PPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 116

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLSTVSISYPSMFSNSAFG+QVFNADWT+SENPSSVSQKPHIISSFSD
Sbjct: 117 QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSENPSSVSQKPHIISSFSD 176

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSLTLDIPSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTVTLNNN
Sbjct: 177 LSLTLDIPSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNN 236

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIY+SSPINLTHSLSQITSGGF GIVRIAVLP+PHCET+LDRF SCYPVSGE NFRN
Sbjct: 237 QTWLIYASSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRN 296

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           P  LEYKWEKKGWGNLLMLAHPLHLRLL+ + S AVVLD FKYKSIDGDLVGVVGGSWVL
Sbjct: 297 PFSLEYKWEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVL 356

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPV VTWHSING+GEEF SEIISALVKDVEGLKSSPITTT SYFYGKSIARAARLALI
Sbjct: 357 KPDPVYVTWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALI 416

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVNYL+VIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG++TQQGS DSG DFGFGV
Sbjct: 417 AEEVNYLQVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGV 476

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNSMFPRLRCFDLY
Sbjct: 477 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLY 536

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQESTSEAVN YYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 537 KLHSWASGLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 596

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQIKEGDNLY+ DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITE+L
Sbjct: 597 WWQIKEGDNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEIL 656

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA F KELVDWTLPSLGREGVGEGWKGFAYALQGI+DKDGALE IRNLTGFDDGNSLS
Sbjct: 657 FSDADFVKELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLS 716

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG +E EG G GWKHWWFSH
Sbjct: 717 NLLWWIHSRGGGDEVEGCGGGWKHWWFSH 743

BLAST of Spg010805 vs. ExPASy TrEMBL
Match: E5GCT5 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1298.1 bits (3358), Expect = 0.0e+00
Identity = 632/689 (91.73%), Postives = 649/689 (94.19%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP QPFWQS   P  A  FPQ QSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 57  MSSPSQPFWQS--PPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 116

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLSTVSISYPSMFSNSAFG+QVFNADWT+SENPSSVSQKPHIISSFSD
Sbjct: 117 QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSENPSSVSQKPHIISSFSD 176

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSLTLDIPSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTVTLNNN
Sbjct: 177 LSLTLDIPSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNN 236

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIY+SSPINLTHSLSQITSGGF GIVRIAVLP+PHCET+LDRF SCYPVSGE NFRN
Sbjct: 237 QTWLIYASSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRN 296

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           P  LEYKWEKKGWGNLLMLAHPLHLRLL+ + S AVVLD FKYKSIDGDLVGVVGGSWVL
Sbjct: 297 PFSLEYKWEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVL 356

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPV VTWHSING+GEEF SEIISALVKDVEGLKSSPITTT SYFYGKSIARAARLALI
Sbjct: 357 KPDPVYVTWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALI 416

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVNYL+VIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG++TQQGS DSG DFGFGV
Sbjct: 417 AEEVNYLQVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGV 476

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNSMFPRLRCFDLY
Sbjct: 477 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLY 536

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQESTSEAVN YYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 537 KLHSWASGLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 596

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQIKEGDNLY+ DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITE+L
Sbjct: 597 WWQIKEGDNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEIL 656

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA F KELVDWTLPSLGREGVGEGWKGFAYALQGI+DKDGALE IRNLTGFDDGNSLS
Sbjct: 657 FSDADFVKELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLS 716

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG +E EG G GWKHWWFSH
Sbjct: 717 NLLWWIHSRGGGDEVEGCGGGWKHWWFSH 743

BLAST of Spg010805 vs. ExPASy TrEMBL
Match: A0A1S3CAN8 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498567 PE=3 SV=1)

HSP 1 Score: 1298.1 bits (3358), Expect = 0.0e+00
Identity = 632/689 (91.73%), Postives = 649/689 (94.19%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP QPFWQS   P  A  FPQ QSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 57  MSSPSQPFWQS--PPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 116

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLSTVSISYPSMFSNSAFG+QVFNADWT+SENPSSVSQKPHIISSFSD
Sbjct: 117 QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSENPSSVSQKPHIISSFSD 176

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSLTLDIPSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTVTLNNN
Sbjct: 177 LSLTLDIPSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNN 236

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIY+SSPINLTHSLSQITSGGF GIVRIAVLP+PHCET+LDRF SCYPVSGE NFRN
Sbjct: 237 QTWLIYASSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRN 296

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           P  LEYKWEKKGWGNLLMLAHPLHLRLL+ + S AVVLD FKYKSIDGDLVGVVGGSWVL
Sbjct: 297 PFSLEYKWEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVL 356

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPV VTWHSING+GEEF SEIISALVKDVEGLKSSPITTT SYFYGKSIARAARLALI
Sbjct: 357 KPDPVYVTWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALI 416

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVNYL+VIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG++TQQGS DSG DFGFGV
Sbjct: 417 AEEVNYLQVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGV 476

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNSMFPRLRCFDLY
Sbjct: 477 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLY 536

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQESTSEAVN YYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 537 KLHSWASGLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 596

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQIKEGDNLY+ DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITE+L
Sbjct: 597 WWQIKEGDNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEIL 656

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA F KELVDWTLPSLGREGVGEGWKGFAYALQGI+DKDGALE IRNLTGFDDGNSLS
Sbjct: 657 FSDADFVKELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLS 716

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG +E EG G GWKHWWFSH
Sbjct: 717 NLLWWIHSRGGGDEVEGCGGGWKHWWFSH 743

BLAST of Spg010805 vs. ExPASy TrEMBL
Match: A0A0A0KSJ9 (Endo-1,3(4)-beta-glucanase OS=Cucumis sativus OX=3659 GN=Csa_5G643880 PE=3 SV=1)

HSP 1 Score: 1288.5 bits (3333), Expect = 0.0e+00
Identity = 624/689 (90.57%), Postives = 647/689 (93.90%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP QPFWQS   P  A  FPQ +S+VLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 51  MSSPSQPFWQS--PPPVAALFPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 110

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLSTVS+SYPSM SNSAFGYQVFNADWT+SENP SVSQKPHIISSFSD
Sbjct: 111 QPEYIHPYLIKSSLSTVSVSYPSMLSNSAFGYQVFNADWTVSENPPSVSQKPHIISSFSD 170

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSLTLDIPSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKFTVTLNNN
Sbjct: 171 LSLTLDIPSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNN 230

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIY+SSPINLTHSLSQITSGGF+GIVRIAVLP+PHCET+LDRF SCYPVSGE NFRN
Sbjct: 231 QTWLIYASSPINLTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRN 290

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           P  LEYKWEKKGWGNLLMLAHPLHLRLLS + S+AVVLD FKYKSIDGDLVGVVGGSW L
Sbjct: 291 PFSLEYKWEKKGWGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWAL 350

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPV +TWHSING+GEEF SEIISALVKDVEGLKSSPITTT SYFYGKSIARAARLALI
Sbjct: 351 KPDPVYLTWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALI 410

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVNYL+VIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG++TQQGS DSG DFGFGV
Sbjct: 411 AEEVNYLQVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGV 470

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNSMFPRLRCFDLY
Sbjct: 471 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLY 530

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQESTSEAVN YYSAAL+GLAYGDTHLASIGSTLTA EIKAAQT
Sbjct: 531 KLHSWASGLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQT 590

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQIKEGDNLY+ DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+L
Sbjct: 591 WWQIKEGDNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEIL 650

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA F KELVDWTLPSLGREGVGEGWKGFAYALQG++DKDGALE IRNLTGFDDGNSLS
Sbjct: 651 FSDADFVKELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLS 710

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG +E E  G GWKHWWFSH
Sbjct: 711 NLLWWIHSRGGGDEVEACGGGWKHWWFSH 737

BLAST of Spg010805 vs. ExPASy TrEMBL
Match: A0A6J1GU45 (Endo-1,3(4)-beta-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111456957 PE=3 SV=1)

HSP 1 Score: 1273.1 bits (3293), Expect = 0.0e+00
Identity = 618/689 (89.70%), Postives = 650/689 (94.34%), Query Frame = 0

Query: 1   MSSPLQPFWQSSAAPATATPFPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 60
           MSSP Q F QS   P    PFPQ QSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD
Sbjct: 48  MSSPSQSFRQS--LPPAVAPFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGD 107

Query: 61  QPEYIHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTISENPSSVSQKPHIISSFSD 120
           QPEYIHPYLIKSSLS VSISYPSMFSN+AFGYQVFNADWT+SENP+SVSQKPHIISSFSD
Sbjct: 108 QPEYIHPYLIKSSLSAVSISYPSMFSNTAFGYQVFNADWTVSENPASVSQKPHIISSFSD 167

Query: 121 LSLTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNN 180
           LSL LD PSGNLRFFLVRGSPF+TFEVFNN  ISISTIHAILSFSSNSS TKF+VTLNNN
Sbjct: 168 LSLILDTPSGNLRFFLVRGSPFITFEVFNNTPISISTIHAILSFSSNSSQTKFSVTLNNN 227

Query: 181 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPDPHCETVLDRFYSCYPVSGEANFRN 240
           QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLP+PH E++LDRF SCYPVSGE NFRN
Sbjct: 228 QTWLIYSSSPINLTHSLSQITSGGFSGIVRIAVLPNPHLESILDRFCSCYPVSGEVNFRN 287

Query: 241 PSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSWVL 300
           PS LEYKWEK GWGNLLMLAHPLHLRLLS++DS A+VLD FKYKSIDGDLVGVVGGSWVL
Sbjct: 288 PSSLEYKWEKNGWGNLLMLAHPLHLRLLSSDDS-AIVLDDFKYKSIDGDLVGVVGGSWVL 347

Query: 301 NPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLALI 360
            PDPVS+TWHSINGIGEEF+SEIISALVKDVEGLKSSPITTT SYFYGK+IARAARLALI
Sbjct: 348 KPDPVSITWHSINGIGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKAIARAARLALI 407

Query: 361 AEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGFGV 420
           AEEVN+L+VIPEVRKFLK AIEPWLRGTFNGNGFLYD KWGG++TQQG+SDSG DFGFGV
Sbjct: 408 AEEVNFLQVIPEVRKFLKDAIEPWLRGTFNGNGFLYDSKWGGLVTQQGASDSGADFGFGV 467

Query: 421 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRRSNSMFPRLRCFDLY 480
           YNDHHYHIGYFLYAIAVLVKIDPAWGRKF+ QAYSLMADFMNLSRRS+S FPRLRCFDLY
Sbjct: 468 YNDHHYHIGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRSSSKFPRLRCFDLY 527

Query: 481 KLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEIKAAQT 540
           KLHSWASGLTEFADGRNQES+SEAVNAYYSAAL+GLAYGDTHLAS+GSTLTA EIKAAQT
Sbjct: 528 KLHSWASGLTEFADGRNQESSSEAVNAYYSAALLGLAYGDTHLASVGSTLTALEIKAAQT 587

Query: 541 WWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVL 600
           WWQI+ G+NLY+TDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQ+LPLLPITE L
Sbjct: 588 WWQIRGGENLYETDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQLLPLLPITEAL 647

Query: 601 FSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDDGNSLS 660
           FSDA FAKELVDWTLPSL REGVGEGWKGFAYALQGI+DKDGA++NIRNL GFDDG+SLS
Sbjct: 648 FSDAAFAKELVDWTLPSLRREGVGEGWKGFAYALQGIYDKDGAVQNIRNLKGFDDGSSLS 707

Query: 661 NLLWWIHSRGGEEEGEGFGRGWKHWWFSH 690
           NLLWWIHSRGG EEGEGFG GWKHWWFSH
Sbjct: 708 NLLWWIHSRGGGEEGEGFGGGWKHWWFSH 733

BLAST of Spg010805 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 943.7 bits (2438), Expect = 8.1e-275
Identity = 465/704 (66.05%), Postives = 554/704 (78.69%), Query Frame = 0

Query: 7   PFWQSSAAPATATP--FPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEY 66
           P  Q S++    TP  FP++ S+VLPDPS FF+  LLSSPLPTNSFFQNFT+ NGDQ EY
Sbjct: 41  PSQQMSSSRQKNTPFLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEY 100

Query: 67  IHPYLIKSSLSTVSISYPSMFSNSAFGYQVFNADWTI--SENPSSVSQKPHIISSFSDLS 126
            HPY+IK S S++SISYPS+  NSAF Y+ FNAD TI  S+ P   S+K H+ISSFSDL 
Sbjct: 101 FHPYIIKPSTSSLSISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLG 160

Query: 127 LTLDIPSGNLRFFLVRGSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNNQT 186
           +TLD PS NLRFFLVRGSPF+TF V  N+ I+ISTIHA+LS S N+SSTK+TV LNNNQT
Sbjct: 161 VTLDFPSSNLRFFLVRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKLNNNQT 220

Query: 187 WLIYSSSPINLT-HSLSQITSG-GFSGIVRIAVL--PDPHCETVLDRFYSCYPVSGEANF 246
           WLIY+SSPINLT   +S I  G GFSGI+RI VL  P+P+ ET+LD F   YPVSG+A+F
Sbjct: 221 WLIYASSPINLTKDGVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADF 280

Query: 247 RNPSCLEYKWEKKGWGNLLMLAHPLHLRLLSNEDSAAVVLDGFKYKSIDGDLVGVVGGSW 306
             P  LEYKWEK+G+G+LLMLAHPLHL+LLS  D +  VLD FKY SIDGDLVGV+G SW
Sbjct: 281 TKPFALEYKWEKRGYGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSW 340

Query: 307 VLNPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARAARLA 366
           VL PDPVSVTWHSI G+ E+   EIISAL+KDV  L SS   T  SYFY K IARAARLA
Sbjct: 341 VLKPDPVSVTWHSIKGVQEDSHQEIISALIKDVNALDSSAEVTNSSYFYAKLIARAARLA 400

Query: 367 LIAEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGKDFGF 426
           LIAEEV YL+VIP++R +LK  IEPWL G+F  NGFLYD KWGG++T+ GS DSG DFGF
Sbjct: 401 LIAEEVCYLDVIPKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGF 460

Query: 427 GVYNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSR---RSNSMFPRLR 486
           G+YNDHHYH+GYF+YAIAVL KIDP WG++++ QAY+LMAD++ L +   +SNS +PRLR
Sbjct: 461 GIYNDHHYHLGYFVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLR 520

Query: 487 CFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTLTAFEI 546
           CFDL+KLHSWA GLTEFADGRNQESTSEAVNAYYSAAL+GLAYGDTHL +  S +   EI
Sbjct: 521 CFDLFKLHSWAGGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEI 580

Query: 547 KAAQTWWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLP 606
            AA+ WWQ+KE D +Y  DF  EN+VVGVLWS KRDSGLWFAP +W+ECRLGIQ+LPLLP
Sbjct: 581 HAAKMWWQVKEDDAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLP 640

Query: 607 ITEVLFSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNLTGFDD 666
           ++EVLFSD  F K+LV+WT+P+L R+ VGEGWKGF YAL+ ++DKDGA+E I+ L GFDD
Sbjct: 641 VSEVLFSDVTFVKQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDD 700

Query: 667 GNSLSNLLWWIHSRGGEEEGE----------GFGRGWKHWWFSH 690
           GNSLSNLLWW+HSR  +++ +          G G G K+  F H
Sbjct: 701 GNSLSNLLWWVHSRNNDDDDDYEEDDEGGYGGHGGGGKYCSFGH 742

BLAST of Spg010805 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 827.8 bits (2137), Expect = 6.5e-240
Identity = 422/679 (62.15%), Postives = 495/679 (72.90%), Query Frame = 0

Query: 21  FPQAQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSSLSTVSIS 80
           FP+ +S+VLPDPS FF+P LLS+PLPTNSFFQNFT+KNGDQ EY HPYLIKS  S++ IS
Sbjct: 10  FPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLCIS 69

Query: 81  YPSMFSNSAFGYQVFNADWTI--SENPSSVSQKPHIISSFSDLSLTLDIPSGNLRFFLVR 140
           YPS+F NS F Y+VF AD  I  S  P   S+K HIISSFSDL +TLD PS NLRFFLVR
Sbjct: 70  YPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFLVR 129

Query: 141 GSPFLTFEVFNNALISISTIHAILSFSSNSSSTKFTVTLNNNQTWLIYSSSPINLT-HSL 200
           GSP                          SSSTK++  L NNQ WLIY+SSPI+LT H  
Sbjct: 130 GSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKHGD 189

Query: 201 SQI-TSGGFSGIVRIAVLP--DPHCETVLDRFYSCYPVSGEANFRNPSCLEYKWEKKGWG 260
           S I   GGF+GIVRI VLP  +P  E+ LDRF SCYPVSG+A+F  P  L+Y WEK+G G
Sbjct: 190 SSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRGSG 249

Query: 261 NLLMLAHPLHLRLLSNE--------------------DSAAVVLDGFKYKSIDGDLVGVV 320
           +LLMLAHPLHL+LL+ +                    +S+  VLD F+YKSIDGDLVGVV
Sbjct: 250 DLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVGVV 309

Query: 321 GGSWVLNPDPVSVTWHSINGIGEEFRSEIISALVKDVEGLKSSPITTTESYFYGKSIARA 380
           G SWVL PD VSVTWHS+ G+ ++   EIISAL KDV GL SS   T+ SYFYGK IARA
Sbjct: 310 GDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEVTSSSYFYGKLIARA 369

Query: 381 ARLALIAEEVNYLEVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGILTQQGSSDSGK 440
           AR ALIAEEV YL+VIP++  +LK  IEPWL G+F  NGFLYD KWGG++T+QGS DS  
Sbjct: 370 ARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSKDSQA 429

Query: 441 DFGFGVYNDHHYHIGYFLYAIAVLVKIDPAWGRKFKSQAYSLMADFMNLSRR----SNSM 500
           DFGFG+YNDHHYHIGYFLYAIAVL K DP WG ++++QAYSL+ADFM   R+    SNS 
Sbjct: 430 DFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNNSNSS 489

Query: 501 FPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALMGLAYGDTHLASIGSTL 560
           +PRLR FDL+KLHSWA GLTEF DGRNQESTSEAVNAYYSAAL+GLAYGD HL    ST+
Sbjct: 490 YPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVETASTI 549

Query: 561 TAFEIKAAQTWWQIKEGDNLYDTDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQV 620
              EI AA+ WWQ+K+G+ LY  DF  EN+VVGVLWS KRDS LWF P +W+ECRLGIQ+
Sbjct: 550 MTLEIHAAKMWWQVKKGEALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECRLGIQL 609

Query: 621 LPLLPITEVLFSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALENIRNL 670
           LP+LP              LV+WTLP+L R GVGEGWKGF YAL+ ++DKDGA++ I+ L
Sbjct: 610 LPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIKKIKRL 648