Spg010653 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg010653
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
Locationscaffold2: 2254543 .. 2256414 (-)
RNA-Seq ExpressionSpg010653
SyntenySpg010653
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGCGTCTGCCAACTTCTTCAAGGACATTGACCACACATGGGGAGACGACAGAGTTCAAATACTCAACAATGGCGAAGTCATAACTGTCACTCTCGATGACACCTCGGGCTCCGGATTCCGATCCCGAGACGAGTATCTCTTTGCCAAGATTGATCTGCAAATCAAGCTCGTTCCTGGAAACTCTGCTGGCACCGTTACTGCATTTTACGTGAGTGTTATTGAAGATTTTTCTTCTGTTTTAGTATGTGATGAAATTAAAGAGGCATATGAAAATTGGGGTTTTTGTTTTGGGATTATAGTTGTCTTCTACAGGGCCATTCCATGATGAGATAGACTTTGAATTCTTGGGGAGAGTTGAAGGGGATCCTTACATTCTGCAGACCAACATGTTCATCCATGGAGTCGGCAAAAGAGAGACACAATTTTCCTTTGGTTTTGACCCACAGCTGATTTTCACACCTACACCATTCTCTGGACTCCTCTTCATATCGTGTGAGTAAATCAAACCTAAACACATACGTTCAAATACCATTTTGATTCAATGTTACCAAATTTTACATGTCCAAGTTTAATGAATTTTTAGAGAAAATATCAAATTTAACCTTGAACTTAGGAAGGTGTATCAATTTGTGCCTAAACTTTCAATTTTCATCAAATTGCACCCTAAACTTGACAATATTGCAATTTTAACCTTAAAACTTAATCAGTGTTGTAACTTTTAACCCTTTTGATAGACATTGATTTTTTTTTTTTTTTAGAAAATGATACTTTATTAAGATGTATGTGAGAAAAATACAAGAGAGGAGTAACATGAAAGCCTTCCAGTTAAAAGGTATAAAAGATGTATCATAGTTTGATTTGGAAAACTGCTCATTTGGAAACTGCTCATGAAAATATTTTCATGTGCAATGGATATAAAACATATGTTAATAAAGCTTCAAAGACAATTTGGCTTTAGGGTTGGATTTTTGTAGAAATTTGTAAGCTTTGAGGTTTTTTTTTTTTTAGTTCAACAACTTTTGAGATATTTTCTACGTTACTATTTGATTGGATGAAGTTAAAAGTTTAGACTTCAGAGAATATTGATATAACTTAACCTCCCTGGAAAACATTTTTTTGGAGTAAAAATGGAAATTAACTTTAAATAATTGTGGGCTATTTTAAAGACTAACAGAAGTACATATAAACTAAATCTTGACAAATGAAAAATAAGATAAATCTCGACAGAGAGGGGCTAAAATGGTATTTAAATCTCCTTGATATGAATTATGACATGAGTAATAATCAAACCCAAATATATGGGTTAAAAACATACCATTTTGGACTCTATATATCATGAAAATTAATTACAAAGTAATGTCAAAAGACTATATTCAAGATTGATTAAAATAAAATCATAGGGGCTAATTAATTCGAGCATGAAAAGGGAAGGAAGGAAAATAATAATAATAAAAATAAAAACTAAGATATATGTATTTGTTTGATTTGAGACAGGCTGTACGTTGACGGGATTCCGATCAGAGAGTTGAAGAACAAGAAGGAAAAGGGAGCTCCATTCCCAGTCCGGCAGCCGATGAGGATATACGCCAGCCTGTGGAATGCAGACAGCTGGGCCACGAGAGGCGGCGCCGTCAAGATCGACTGGACCAAAGCGCCGTTCCGAGCATGGTACAAGAATCTAAGCGCCGATGGCTGCTTGTGGTCCGACGGCAGATCCAATTGCTCTTCAACTTCAGCCTCGTGGCTCTCATCCACGCTTGATTCTGTAAGCCAACGCCACATGAAATCTGCTCAGACCAAATATTTGTTCTATGATTACTGCACTGACACCAACCGTTTCCCTCTGGGTCTTCCTCTTGAATGTTGA

mRNA sequence

ATGGTTGCGTCTGCCAACTTCTTCAAGGACATTGACCACACATGGGGAGACGACAGAGTTCAAATACTCAACAATGGCGAAGTCATAACTGTCACTCTCGATGACACCTCGGGCTCCGGATTCCGATCCCGAGACGAGTATCTCTTTGCCAAGATTGATCTGCAAATCAAGCTCGTTCCTGGAAACTCTGCTGGCACCGTTACTGCATTTTACTTGTCTTCTACAGGGCCATTCCATGATGAGATAGACTTTGAATTCTTGGGGAGAGTTGAAGGGGATCCTTACATTCTGCAGACCAACATGTTCATCCATGGAGTCGGCAAAAGAGAGACACAATTTTCCTTTGGTTTTGACCCACAGCTGATTTTCACACCTACACCATTCTCTGGACTCCTCTTCATATCGTacaggctgtacgttgacgggattccgatcagagagttgaagaacaagaaggaaaagggagctccattcccagtccggcagccgatgaggatatacgccagcctgtggaatgcagacagctgggccacgagaggcggcgccgtcaagatcgactggaccaaagcgccgttccgagcatggtacaagaatctaagcgccgatggctgcttgtggtccgacggcagatccaattgctcttcaacttcagcctcgtggctctcatccacgcttgattctgtaagccaacgccacatgaaatctgctcagaccaaatatttgttctatgattactgcactgacaccaaccgtttccctctgggtcttcctcttgaatgttga

Coding sequence (CDS)

ATGGTTGCGTCTGCCAACTTCTTCAAGGACATTGACCACACATGGGGAGACGACAGAGTTCAAATACTCAACAATGGCGAAGTCATAACTGTCACTCTCGATGACACCTCGGGCTCCGGATTCCGATCCCGAGACGAGTATCTCTTTGCCAAGATTGATCTGCAAATCAAGCTCGTTCCTGGAAACTCTGCTGGCACCGTTACTGCATTTTACTTGTCTTCTACAGGGCCATTCCATGATGAGATAGACTTTGAATTCTTGGGGAGAGTTGAAGGGGATCCTTACATTCTGCAGACCAACATGTTCATCCATGGAGTCGGCAAAAGAGAGACACAATTTTCCTTTGGTTTTGACCCACAGCTGATTTTCACACCTACACCATTCTCTGGACTCCTCTTCATATCGTACAGGCTGTACGTTGACGGGATTCCGATCAGAGAGTTGAAGAACAAGAAGGAAAAGGGAGCTCCATTCCCAGTCCGGCAGCCGATGAGGATATACGCCAGCCTGTGGAATGCAGACAGCTGGGCCACGAGAGGCGGCGCCGTCAAGATCGACTGGACCAAAGCGCCGTTCCGAGCATGGTACAAGAATCTAAGCGCCGATGGCTGCTTGTGGTCCGACGGCAGATCCAATTGCTCTTCAACTTCAGCCTCGTGGCTCTCATCCACGCTTGATTCTGTAAGCCAACGCCACATGAAATCTGCTCAGACCAAATATTTGTTCTATGATTACTGCACTGACACCAACCGTTTCCCTCTGGGTCTTCCTCTTGAATGTTGA

Protein sequence

MVASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQLIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKSAQTKYLFYDYCTDTNRFPLGLPLEC
Homology
BLAST of Spg010653 vs. NCBI nr
Match: XP_022933964.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita moschata])

HSP 1 Score: 452.2 bits (1162), Expect = 3.0e-123
Identity = 219/265 (82.64%), Postives = 232/265 (87.55%), Query Frame = 0

Query: 3   ASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGN 62
           ASANF+K+IDHTWGDDRVQIL+NGEVI VTLD TSGSGF+SR EYLFAKIDLQIKLVPGN
Sbjct: 32  ASANFYKNIDHTWGDDRVQILDNGEVIRVTLDQTSGSGFQSRGEYLFAKIDLQIKLVPGN 91

Query: 63  SAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP--- 122
           SAGTVTAFY+SSTG FHDEIDFEFLGRVEGDPYILQTNMF+HGVGKRETQF   FDP   
Sbjct: 92  SAGTVTAFYVSSTGEFHDEIDFEFLGRVEGDPYILQTNMFLHGVGKRETQFFLWFDPTAD 151

Query: 123 ----QLIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADS 182
                +++TP            L VDGIPIRELKNKKEKGAPFP+RQPMRIYASLWNADS
Sbjct: 152 FHNYTILWTPRHIV--------LSVDGIPIRELKNKKEKGAPFPLRQPMRIYASLWNADS 211

Query: 183 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKS 242
           WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDG+SNCSS+SASWLSSTLD VSQR MK 
Sbjct: 212 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGKSNCSSSSASWLSSTLDRVSQRRMKW 271

Query: 243 AQTKYLFYDYCTDTNRFPLGLPLEC 261
           AQTKY+FYDYCTDT RFP GLPLEC
Sbjct: 272 AQTKYMFYDYCTDTKRFPQGLPLEC 288

BLAST of Spg010653 vs. NCBI nr
Match: KAG6587762.1 (Xyloglucan endotransglucosylase/hydrolase protein 22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 452.2 bits (1162), Expect = 3.0e-123
Identity = 219/265 (82.64%), Postives = 232/265 (87.55%), Query Frame = 0

Query: 3   ASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGN 62
           ASANF+K+IDHTWGDDRVQIL+NGEVI VTLD TSGSGF+SR EYLFAKIDLQIKLVPGN
Sbjct: 15  ASANFYKNIDHTWGDDRVQILDNGEVIRVTLDQTSGSGFQSRGEYLFAKIDLQIKLVPGN 74

Query: 63  SAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP--- 122
           SAGTVTAFY+SSTG FHDEIDFEFLGRVEGDPYILQTNMF+HGVGKRETQF   FDP   
Sbjct: 75  SAGTVTAFYVSSTGEFHDEIDFEFLGRVEGDPYILQTNMFLHGVGKRETQFFLWFDPTAD 134

Query: 123 ----QLIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADS 182
                +++TP            L VDGIPIRELKNKKEKGAPFP+RQPMRIYASLWNADS
Sbjct: 135 FHNYTILWTPRHIV--------LSVDGIPIRELKNKKEKGAPFPLRQPMRIYASLWNADS 194

Query: 183 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKS 242
           WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDG+SNCSS+SASWLSSTLD VSQR MK 
Sbjct: 195 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGKSNCSSSSASWLSSTLDRVSQRRMKW 254

Query: 243 AQTKYLFYDYCTDTNRFPLGLPLEC 261
           AQTKY+FYDYCTDT RFP GLPLEC
Sbjct: 255 AQTKYMFYDYCTDTKRFPQGLPLEC 271

BLAST of Spg010653 vs. NCBI nr
Match: XP_023003613.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita maxima])

HSP 1 Score: 452.2 bits (1162), Expect = 3.0e-123
Identity = 218/265 (82.26%), Postives = 233/265 (87.92%), Query Frame = 0

Query: 3   ASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGN 62
           ASANF+K+IDHTWGDDRVQIL+NGEVI VTLD TSGSGF+SR EYLFAKIDLQIKLVPGN
Sbjct: 32  ASANFYKNIDHTWGDDRVQILDNGEVIRVTLDQTSGSGFQSRGEYLFAKIDLQIKLVPGN 91

Query: 63  SAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP--- 122
           SAGTVTAFY+SSTG FHDEIDFEFLGRVEGDPYILQTNMF+HGVGKRETQF   FDP   
Sbjct: 92  SAGTVTAFYVSSTGEFHDEIDFEFLGRVEGDPYILQTNMFLHGVGKRETQFFLWFDPTAD 151

Query: 123 ----QLIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADS 182
                +++TP        +   L VDGIPIRELKN+KEKGAPFP+RQPMRIYASLWNADS
Sbjct: 152 FHNYTILWTP--------LHIVLSVDGIPIRELKNRKEKGAPFPLRQPMRIYASLWNADS 211

Query: 183 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKS 242
           WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDG+SNCSSTSASWLSSTLD +SQR MK 
Sbjct: 212 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGKSNCSSTSASWLSSTLDRLSQRRMKW 271

Query: 243 AQTKYLFYDYCTDTNRFPLGLPLEC 261
           AQTKY+FYDYCTDT RFP GLPLEC
Sbjct: 272 AQTKYMFYDYCTDTKRFPQGLPLEC 288

BLAST of Spg010653 vs. NCBI nr
Match: XP_023531496.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 451.8 bits (1161), Expect = 3.9e-123
Identity = 218/265 (82.26%), Postives = 233/265 (87.92%), Query Frame = 0

Query: 3   ASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGN 62
           ASANF+K+IDHTWGDDRVQIL+NG+VI VTLD TSGSGF+SR EYLFAKIDLQIKLVPGN
Sbjct: 32  ASANFYKNIDHTWGDDRVQILDNGQVIRVTLDQTSGSGFQSRGEYLFAKIDLQIKLVPGN 91

Query: 63  SAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP--- 122
           SAGTVTAFY+SSTG FHDEIDFEFLGRVEGDPYILQTNMF+HGVGKRETQF   FDP   
Sbjct: 92  SAGTVTAFYVSSTGEFHDEIDFEFLGRVEGDPYILQTNMFLHGVGKRETQFFLWFDPTAD 151

Query: 123 ----QLIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADS 182
                +++TP        +   L VDGIPIRELKNKKEKGAPFP+RQPMRIYASLWNADS
Sbjct: 152 FHNYTILWTP--------LHIVLSVDGIPIRELKNKKEKGAPFPLRQPMRIYASLWNADS 211

Query: 183 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKS 242
           WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDG+SNCSS+SASWLSSTLD VSQR MK 
Sbjct: 212 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGKSNCSSSSASWLSSTLDRVSQRRMKW 271

Query: 243 AQTKYLFYDYCTDTNRFPLGLPLEC 261
           AQTKY+FYDYCTDT RFP GLPLEC
Sbjct: 272 AQTKYMFYDYCTDTKRFPQGLPLEC 288

BLAST of Spg010653 vs. NCBI nr
Match: XP_038880549.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Benincasa hispida])

HSP 1 Score: 421.8 bits (1083), Expect = 4.3e-114
Identity = 206/266 (77.44%), Postives = 220/266 (82.71%), Query Frame = 0

Query: 1   MVASANFFKDIDHTW-GDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLV 60
           MVASANF KDIDHTW G DRVQILNNGE I + LD+TS SGF+SR++YLFAKIDLQIKLV
Sbjct: 32  MVASANFLKDIDHTWGGGDRVQILNNGEGIAIALDETSCSGFQSRNQYLFAKIDLQIKLV 91

Query: 61  PGNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP 120
           PGNSAGTVTAFY+SS   FHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQF   FDP
Sbjct: 92  PGNSAGTVTAFYVSSKEEFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFFLWFDP 151

Query: 121 QLIFTP-----TPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNAD 180
              F       TPF  +      L VDGIPIRELKN KEKGAPFP+RQPMRIY SLWNAD
Sbjct: 152 TTDFHNYTILWTPFHIV------LSVDGIPIRELKNNKEKGAPFPMRQPMRIYGSLWNAD 211

Query: 181 SWATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMK 240
           SWATRGGAVKIDWTK PFRAW+KNL  DGCLWSDG SNC+ +S SWLSSTLD+ SQ+HMK
Sbjct: 212 SWATRGGAVKIDWTKGPFRAWFKNLRVDGCLWSDGNSNCTKSSTSWLSSTLDNESQQHMK 271

Query: 241 SAQTKYLFYDYCTDTNRFPLGLPLEC 261
            A T Y+FYDYCTDT RFP GLPLEC
Sbjct: 272 WAHTNYMFYDYCTDTKRFPKGLPLEC 291

BLAST of Spg010653 vs. ExPASy Swiss-Prot
Match: Q38857 (Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=3702 GN=XTH22 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 7.3e-80
Identity = 142/265 (53.58%), Postives = 181/265 (68.30%), Query Frame = 0

Query: 4   SANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGNS 63
           SANF +D++ TWGD R QI NNGE++T++LD +SGSGF+S++EYLF K+ +Q+KLVPGNS
Sbjct: 20  SANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSKNEYLFGKVSMQMKLVPGNS 79

Query: 64  AGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQLIF 123
           AGTVT  YL S G   DEIDFEFLG   G+PY L TN++  G G +E QF   FDP   F
Sbjct: 80  AGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQGKGDKEQQFKLWFDPTANF 139

Query: 124 TPTPFSGLLFISYRLY--VDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWATRGG 183
                  +L+   R+   VDG PIRE KN +  G  FP  +PMR+Y+SLWNAD WATRGG
Sbjct: 140 HTYT---ILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRMYSSLWNADDWATRGG 199

Query: 184 AVKIDWTKAPFRAWYKNLSADGCLWSDGRSNC------SSTSASWLSSTLDSVSQRHMKS 243
            VK DW+KAPF A Y+    + C+WS+G+S+C       +T+ SWLS  LDS +Q+ M+ 
Sbjct: 200 LVKTDWSKAPFTASYRGFQQEACVWSNGKSSCPNASKQGTTTGSWLSQELDSTAQQRMRW 259

Query: 244 AQTKYLFYDYCTDTNRFPLGLPLEC 261
            Q  Y+ Y+YCTD  RFP GLP EC
Sbjct: 260 VQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of Spg010653 vs. ExPASy Swiss-Prot
Match: Q38910 (Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis thaliana OX=3702 GN=XTH23 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 7.3e-80
Identity = 145/264 (54.92%), Postives = 180/264 (68.18%), Query Frame = 0

Query: 4   SANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGNS 63
           SANF +D++ TWGD R QI NNG+++T++LD  SGSGF+S++EYLF KID+QIKLV GNS
Sbjct: 23  SANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYLFGKIDMQIKLVAGNS 82

Query: 64  AGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQLIF 123
           AGTVTA+YL S G   DEIDFEFLG + GDPY L TN+F  G G RE QF   FDP   F
Sbjct: 83  AGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDREQQFKLWFDPTSDF 142

Query: 124 TPTPFSGLLFISYRLY--VDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWATRGG 183
                  +L+   R+   VDG PIRE KN + +G  FP  QPMR+Y+SLWNA+ WATRGG
Sbjct: 143 HT---YSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSLWNAEEWATRGG 202

Query: 184 AVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTS-----ASWLSSTLDSVSQRHMKSA 243
            VK DW+KAPF A Y+  + + C+  +G+S+C + S      SWLS  LDS  Q  M+  
Sbjct: 203 LVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQELDSTGQEQMRWV 262

Query: 244 QTKYLFYDYCTDTNRFPLGLPLEC 261
           Q  Y+ Y+YCTD  RFP GLP EC
Sbjct: 263 QNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of Spg010653 vs. ExPASy Swiss-Prot
Match: Q38907 (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana OX=3702 GN=XTH25 PE=2 SV=2)

HSP 1 Score: 295.8 bits (756), Expect = 4.7e-79
Identity = 141/260 (54.23%), Postives = 178/260 (68.46%), Query Frame = 0

Query: 2   VASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPG 61
           V +  F  + D TWGD R ++LNNGE++T++LD  SGSGF+++ EYLF KID+Q+KLVPG
Sbjct: 25  VFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKKEYLFGKIDMQLKLVPG 84

Query: 62  NSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQL 121
           NSAGTVTA+YL S G   DEIDFEFLG + GDPY + TN++  G G RE QF   FDP  
Sbjct: 85  NSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQGKGDREQQFHLWFDPTA 144

Query: 122 IFTPTPFSGLLFISYRLY-VDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWATRG 181
            F    +S L    + ++ VD IP+RE KN +  G  +P  QPMR+Y+SLWNAD WATRG
Sbjct: 145 DF--HTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRLYSSLWNADQWATRG 204

Query: 182 GAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKSAQTKY 241
           G VK DW+KAPF A Y+N  AD C+ S GRS+C + S  W S  LD  ++  M+  Q KY
Sbjct: 205 GLVKTDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPRWFSQRLDLTAEDKMRVVQRKY 264

Query: 242 LFYDYCTDTNRFPLGLPLEC 261
           + Y+YCTDT RFP G P EC
Sbjct: 265 MIYNYCTDTKRFPQGFPKEC 282

BLAST of Spg010653 vs. ExPASy Swiss-Prot
Match: O80803 (Probable xyloglucan endotransglucosylase/hydrolase protein 17 OS=Arabidopsis thaliana OX=3702 GN=XTH17 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 9.8e-77
Identity = 138/265 (52.08%), Postives = 182/265 (68.68%), Query Frame = 0

Query: 2   VASANFFKDIDHTWGDDRVQILN-NGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVP 61
           V + +F KD+   WGD R +I + +G++++++LD +SGSGF+S  E+L+ K ++Q+KLVP
Sbjct: 24  VYAGSFHKDVQIHWGDGRGKIHDRDGKLLSLSLDKSSGSGFQSNQEFLYGKAEVQMKLVP 83

Query: 62  GNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQ 121
           GNSAGTVT FYL S G   DEIDFEFLG + G PY L TN++  G G +E QF   FDP 
Sbjct: 84  GNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGDKEQQFHLWFDPT 143

Query: 122 LIF-----TPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADS 181
           + F     T  P   ++F      VDGIPIRE KN +  G PFP RQPMR+YASLW A+ 
Sbjct: 144 VNFHTYCITWNP-QRIIFT-----VDGIPIREFKNPEAIGVPFPTRQPMRLYASLWEAEH 203

Query: 182 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKS 241
           WATRGG  K DW+KAPF A+Y+N + DGC+W++G+S+CS+ S  W +  LDS  Q  MK 
Sbjct: 204 WATRGGLEKTDWSKAPFTAFYRNYNVDGCVWANGKSSCSANS-PWFTQKLDSNGQTRMKG 263

Query: 242 AQTKYLFYDYCTDTNRFPLGLPLEC 261
            Q+KY+ Y+YCTD  RFP G+P EC
Sbjct: 264 VQSKYMIYNYCTDKRRFPRGVPAEC 281

BLAST of Spg010653 vs. ExPASy Swiss-Prot
Match: Q9FI31 (Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=3702 GN=XTH20 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.1e-75
Identity = 135/262 (51.53%), Postives = 179/262 (68.32%), Query Frame = 0

Query: 2   VASANFFKDIDHTWGDDRVQILNN-GEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVP 61
           V + +F KD+   WGD R +IL+N G +++++LD  SGSGF+S  E+L+ K+++Q+KLVP
Sbjct: 24  VYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQEFLYGKVEVQMKLVP 83

Query: 62  GNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQ 121
           GNSAGTVT FYL S G   DEIDFEFLG + G PY L TN++  G G +E QF   FDP 
Sbjct: 84  GNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGDKEQQFHLWFDPT 143

Query: 122 LIFTPTPFSGLLFISYRLY--VDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWAT 181
           + F       +++   R+   +DGIPIRE KN +  G PFP  QPMR+YASLW A+ WAT
Sbjct: 144 VDFHT---YCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLYASLWEAEHWAT 203

Query: 182 RGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKSAQT 241
           RGG  K DW+KAPF A+Y+N + D C+WS+G+S+CS+ S SW +  LD   +  +K AQ 
Sbjct: 204 RGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANS-SWFTQVLDFKGKNRVKWAQR 263

Query: 242 KYLFYDYCTDTNRFPLGLPLEC 261
           KY+ Y+YCTD  RFP G P EC
Sbjct: 264 KYMVYNYCTDKKRFPQGAPPEC 281

BLAST of Spg010653 vs. ExPASy TrEMBL
Match: A0A6J1KX23 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497159 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.5e-123
Identity = 218/265 (82.26%), Postives = 233/265 (87.92%), Query Frame = 0

Query: 3   ASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGN 62
           ASANF+K+IDHTWGDDRVQIL+NGEVI VTLD TSGSGF+SR EYLFAKIDLQIKLVPGN
Sbjct: 32  ASANFYKNIDHTWGDDRVQILDNGEVIRVTLDQTSGSGFQSRGEYLFAKIDLQIKLVPGN 91

Query: 63  SAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP--- 122
           SAGTVTAFY+SSTG FHDEIDFEFLGRVEGDPYILQTNMF+HGVGKRETQF   FDP   
Sbjct: 92  SAGTVTAFYVSSTGEFHDEIDFEFLGRVEGDPYILQTNMFLHGVGKRETQFFLWFDPTAD 151

Query: 123 ----QLIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADS 182
                +++TP        +   L VDGIPIRELKN+KEKGAPFP+RQPMRIYASLWNADS
Sbjct: 152 FHNYTILWTP--------LHIVLSVDGIPIRELKNRKEKGAPFPLRQPMRIYASLWNADS 211

Query: 183 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKS 242
           WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDG+SNCSSTSASWLSSTLD +SQR MK 
Sbjct: 212 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGKSNCSSTSASWLSSTLDRLSQRRMKW 271

Query: 243 AQTKYLFYDYCTDTNRFPLGLPLEC 261
           AQTKY+FYDYCTDT RFP GLPLEC
Sbjct: 272 AQTKYMFYDYCTDTKRFPQGLPLEC 288

BLAST of Spg010653 vs. ExPASy TrEMBL
Match: A0A6J1F180 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111441218 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.5e-123
Identity = 219/265 (82.64%), Postives = 232/265 (87.55%), Query Frame = 0

Query: 3   ASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGN 62
           ASANF+K+IDHTWGDDRVQIL+NGEVI VTLD TSGSGF+SR EYLFAKIDLQIKLVPGN
Sbjct: 32  ASANFYKNIDHTWGDDRVQILDNGEVIRVTLDQTSGSGFQSRGEYLFAKIDLQIKLVPGN 91

Query: 63  SAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP--- 122
           SAGTVTAFY+SSTG FHDEIDFEFLGRVEGDPYILQTNMF+HGVGKRETQF   FDP   
Sbjct: 92  SAGTVTAFYVSSTGEFHDEIDFEFLGRVEGDPYILQTNMFLHGVGKRETQFFLWFDPTAD 151

Query: 123 ----QLIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADS 182
                +++TP            L VDGIPIRELKNKKEKGAPFP+RQPMRIYASLWNADS
Sbjct: 152 FHNYTILWTPRHIV--------LSVDGIPIRELKNKKEKGAPFPLRQPMRIYASLWNADS 211

Query: 183 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKS 242
           WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDG+SNCSS+SASWLSSTLD VSQR MK 
Sbjct: 212 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGKSNCSSSSASWLSSTLDRVSQRRMKW 271

Query: 243 AQTKYLFYDYCTDTNRFPLGLPLEC 261
           AQTKY+FYDYCTDT RFP GLPLEC
Sbjct: 272 AQTKYMFYDYCTDTKRFPQGLPLEC 288

BLAST of Spg010653 vs. ExPASy TrEMBL
Match: A0A5D3CL19 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00690 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.0e-109
Identity = 199/270 (73.70%), Postives = 221/270 (81.85%), Query Frame = 0

Query: 1   MVASANFFKDIDHTW-GDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLV 60
           MV+SANF KDIDHTW G DRVQILNNGE I V+LD+TS SGF+SRD+YLFAKIDL++KLV
Sbjct: 6   MVSSANFLKDIDHTWGGGDRVQILNNGEGIAVSLDETSCSGFQSRDQYLFAKIDLEMKLV 65

Query: 61  PGNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP 120
           PGNSAGTVTAFYLSS G FHDEID EFLGRVEGDPYILQTNMFIHGVG+RE QF   FDP
Sbjct: 66  PGNSAGTVTAFYLSSIGEFHDEIDLEFLGRVEGDPYILQTNMFIHGVGQREMQFFLWFDP 125

Query: 121 Q-------LIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWN 180
                   +++TP            L VDGIPIRELKNK+EK APFP+ QPMRIY S+WN
Sbjct: 126 STDFHNYTILWTPQHIV--------LLVDGIPIRELKNKREKRAPFPMVQPMRIYGSIWN 185

Query: 181 ADSWATRGGAVKIDWTKAPFRAWYKNLSADGCLWSD--GRSNCSSTSASWLSSTLDSVSQ 240
           ADSWATRGGAVKIDWTKAPFRAW+KNL  DGCLWSD   +SNC+ +SASWLSS LD+ S+
Sbjct: 186 ADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDKNSKSNCTKSSASWLSSILDNKSK 245

Query: 241 RHMKSAQTKYLFYDYCTDTNRFPLGLPLEC 261
           + MK AQTKY+FYDYCTDT RFP GLPLEC
Sbjct: 246 QRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 267

BLAST of Spg010653 vs. ExPASy TrEMBL
Match: A0A1S3BXV4 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC103494276 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.0e-109
Identity = 199/270 (73.70%), Postives = 221/270 (81.85%), Query Frame = 0

Query: 1   MVASANFFKDIDHTW-GDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLV 60
           MV+SANF KDIDHTW G DRVQILNNGE I V+LD+TS SGF+SRD+YLFAKIDL++KLV
Sbjct: 33  MVSSANFLKDIDHTWGGGDRVQILNNGEGIAVSLDETSCSGFQSRDQYLFAKIDLEMKLV 92

Query: 61  PGNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP 120
           PGNSAGTVTAFYLSS G FHDEID EFLGRVEGDPYILQTNMFIHGVG+RE QF   FDP
Sbjct: 93  PGNSAGTVTAFYLSSIGEFHDEIDLEFLGRVEGDPYILQTNMFIHGVGQREMQFFLWFDP 152

Query: 121 Q-------LIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWN 180
                   +++TP            L VDGIPIRELKNK+EK APFP+ QPMRIY S+WN
Sbjct: 153 STDFHNYTILWTPQHIV--------LLVDGIPIRELKNKREKRAPFPMVQPMRIYGSIWN 212

Query: 181 ADSWATRGGAVKIDWTKAPFRAWYKNLSADGCLWSD--GRSNCSSTSASWLSSTLDSVSQ 240
           ADSWATRGGAVKIDWTKAPFRAW+KNL  DGCLWSD   +SNC+ +SASWLSS LD+ S+
Sbjct: 213 ADSWATRGGAVKIDWTKAPFRAWFKNLRVDGCLWSDKNSKSNCTKSSASWLSSILDNKSK 272

Query: 241 RHMKSAQTKYLFYDYCTDTNRFPLGLPLEC 261
           + MK AQTKY+FYDYCTDT RFP GLPLEC
Sbjct: 273 QRMKWAQTKYMFYDYCTDTKRFPQGLPLEC 294

BLAST of Spg010653 vs. ExPASy TrEMBL
Match: A0A6J1C4G1 (Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC111007299 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 6.1e-106
Identity = 193/272 (70.96%), Postives = 218/272 (80.15%), Query Frame = 0

Query: 1   MVASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVP 60
           M ASANF++++D  WGD R QIL NGEV++V+LD TSGS FRSR++YL AK+DLQIKLVP
Sbjct: 7   MAASANFYEEVDQIWGDGRAQILKNGEVMSVSLDRTSGSCFRSRNQYLSAKMDLQIKLVP 66

Query: 61  GNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDP- 120
           GNSAGTVTAFY+SS G FHDEIDFEFLGRV+GDPYILQTNMFIHG GKRETQF   FDP 
Sbjct: 67  GNSAGTVTAFYVSSVGEFHDEIDFEFLGRVDGDPYILQTNMFIHGEGKRETQFFLWFDPT 126

Query: 121 ------QLIFTPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNA 180
                  +++TP        +   LYVDGIPIRELKNKKE GAPFP+RQPMR+Y SLWNA
Sbjct: 127 KDFHNYTILWTP--------LHIVLYVDGIPIRELKNKKETGAPFPLRQPMRLYGSLWNA 186

Query: 181 DSWATRGGAVKIDWTKAPFRAWYKNLSADGCLWSD----GRSNCSSTSASWL-SSTLDSV 240
           +SWATRGGAVK DWTKAPFRAW+KNL ADGCLWSD     +SNCSS+SA+WL SSTLD  
Sbjct: 187 ESWATRGGAVKTDWTKAPFRAWFKNLRADGCLWSDHNGKSKSNCSSSSATWLSSSTLDYG 246

Query: 241 SQRHMKSAQTKYLFYDYCTDTNRFPLGLPLEC 261
           SQR MK AQ  ++FYDYC DT RFP GLP EC
Sbjct: 247 SQRRMKWAQRNFMFYDYCNDTARFPQGLPPEC 270

BLAST of Spg010653 vs. TAIR 10
Match: AT4G25810.1 (xyloglucan endotransglycosylase 6 )

HSP 1 Score: 298.5 bits (763), Expect = 5.2e-81
Identity = 145/264 (54.92%), Postives = 180/264 (68.18%), Query Frame = 0

Query: 4   SANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGNS 63
           SANF +D++ TWGD R QI NNG+++T++LD  SGSGF+S++EYLF KID+QIKLV GNS
Sbjct: 23  SANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYLFGKIDMQIKLVAGNS 82

Query: 64  AGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQLIF 123
           AGTVTA+YL S G   DEIDFEFLG + GDPY L TN+F  G G RE QF   FDP   F
Sbjct: 83  AGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDREQQFKLWFDPTSDF 142

Query: 124 TPTPFSGLLFISYRLY--VDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWATRGG 183
                  +L+   R+   VDG PIRE KN + +G  FP  QPMR+Y+SLWNA+ WATRGG
Sbjct: 143 HT---YSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSLWNAEEWATRGG 202

Query: 184 AVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTS-----ASWLSSTLDSVSQRHMKSA 243
            VK DW+KAPF A Y+  + + C+  +G+S+C + S      SWLS  LDS  Q  M+  
Sbjct: 203 LVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQELDSTGQEQMRWV 262

Query: 244 QTKYLFYDYCTDTNRFPLGLPLEC 261
           Q  Y+ Y+YCTD  RFP GLP EC
Sbjct: 263 QNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of Spg010653 vs. TAIR 10
Match: AT5G57560.1 (Xyloglucan endotransglucosylase/hydrolase family protein )

HSP 1 Score: 298.5 bits (763), Expect = 5.2e-81
Identity = 142/265 (53.58%), Postives = 181/265 (68.30%), Query Frame = 0

Query: 4   SANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPGNS 63
           SANF +D++ TWGD R QI NNGE++T++LD +SGSGF+S++EYLF K+ +Q+KLVPGNS
Sbjct: 20  SANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSKNEYLFGKVSMQMKLVPGNS 79

Query: 64  AGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQLIF 123
           AGTVT  YL S G   DEIDFEFLG   G+PY L TN++  G G +E QF   FDP   F
Sbjct: 80  AGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQGKGDKEQQFKLWFDPTANF 139

Query: 124 TPTPFSGLLFISYRLY--VDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWATRGG 183
                  +L+   R+   VDG PIRE KN +  G  FP  +PMR+Y+SLWNAD WATRGG
Sbjct: 140 HTYT---ILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRMYSSLWNADDWATRGG 199

Query: 184 AVKIDWTKAPFRAWYKNLSADGCLWSDGRSNC------SSTSASWLSSTLDSVSQRHMKS 243
            VK DW+KAPF A Y+    + C+WS+G+S+C       +T+ SWLS  LDS +Q+ M+ 
Sbjct: 200 LVKTDWSKAPFTASYRGFQQEACVWSNGKSSCPNASKQGTTTGSWLSQELDSTAQQRMRW 259

Query: 244 AQTKYLFYDYCTDTNRFPLGLPLEC 261
            Q  Y+ Y+YCTD  RFP GLP EC
Sbjct: 260 VQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of Spg010653 vs. TAIR 10
Match: AT5G57550.1 (xyloglucan endotransglucosylase/hydrolase 25 )

HSP 1 Score: 295.8 bits (756), Expect = 3.4e-80
Identity = 141/260 (54.23%), Postives = 178/260 (68.46%), Query Frame = 0

Query: 2   VASANFFKDIDHTWGDDRVQILNNGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVPG 61
           V +  F  + D TWGD R ++LNNGE++T++LD  SGSGF+++ EYLF KID+Q+KLVPG
Sbjct: 25  VFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKKEYLFGKIDMQLKLVPG 84

Query: 62  NSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQL 121
           NSAGTVTA+YL S G   DEIDFEFLG + GDPY + TN++  G G RE QF   FDP  
Sbjct: 85  NSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQGKGDREQQFHLWFDPTA 144

Query: 122 IFTPTPFSGLLFISYRLY-VDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWATRG 181
            F    +S L    + ++ VD IP+RE KN +  G  +P  QPMR+Y+SLWNAD WATRG
Sbjct: 145 DF--HTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRLYSSLWNADQWATRG 204

Query: 182 GAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKSAQTKY 241
           G VK DW+KAPF A Y+N  AD C+ S GRS+C + S  W S  LD  ++  M+  Q KY
Sbjct: 205 GLVKTDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPRWFSQRLDLTAEDKMRVVQRKY 264

Query: 242 LFYDYCTDTNRFPLGLPLEC 261
           + Y+YCTDT RFP G P EC
Sbjct: 265 MIYNYCTDTKRFPQGFPKEC 282

BLAST of Spg010653 vs. TAIR 10
Match: AT1G65310.1 (xyloglucan endotransglucosylase/hydrolase 17 )

HSP 1 Score: 288.1 bits (736), Expect = 7.0e-78
Identity = 138/265 (52.08%), Postives = 182/265 (68.68%), Query Frame = 0

Query: 2   VASANFFKDIDHTWGDDRVQILN-NGEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVP 61
           V + +F KD+   WGD R +I + +G++++++LD +SGSGF+S  E+L+ K ++Q+KLVP
Sbjct: 24  VYAGSFHKDVQIHWGDGRGKIHDRDGKLLSLSLDKSSGSGFQSNQEFLYGKAEVQMKLVP 83

Query: 62  GNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQ 121
           GNSAGTVT FYL S G   DEIDFEFLG + G PY L TN++  G G +E QF   FDP 
Sbjct: 84  GNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGDKEQQFHLWFDPT 143

Query: 122 LIF-----TPTPFSGLLFISYRLYVDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADS 181
           + F     T  P   ++F      VDGIPIRE KN +  G PFP RQPMR+YASLW A+ 
Sbjct: 144 VNFHTYCITWNP-QRIIFT-----VDGIPIREFKNPEAIGVPFPTRQPMRLYASLWEAEH 203

Query: 182 WATRGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKS 241
           WATRGG  K DW+KAPF A+Y+N + DGC+W++G+S+CS+ S  W +  LDS  Q  MK 
Sbjct: 204 WATRGGLEKTDWSKAPFTAFYRNYNVDGCVWANGKSSCSANS-PWFTQKLDSNGQTRMKG 263

Query: 242 AQTKYLFYDYCTDTNRFPLGLPLEC 261
            Q+KY+ Y+YCTD  RFP G+P EC
Sbjct: 264 VQSKYMIYNYCTDKRRFPRGVPAEC 281

BLAST of Spg010653 vs. TAIR 10
Match: AT5G48070.1 (xyloglucan endotransglucosylase/hydrolase 20 )

HSP 1 Score: 284.6 bits (727), Expect = 7.7e-77
Identity = 135/262 (51.53%), Postives = 179/262 (68.32%), Query Frame = 0

Query: 2   VASANFFKDIDHTWGDDRVQILNN-GEVITVTLDDTSGSGFRSRDEYLFAKIDLQIKLVP 61
           V + +F KD+   WGD R +IL+N G +++++LD  SGSGF+S  E+L+ K+++Q+KLVP
Sbjct: 24  VYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQEFLYGKVEVQMKLVP 83

Query: 62  GNSAGTVTAFYLSSTGPFHDEIDFEFLGRVEGDPYILQTNMFIHGVGKRETQFSFGFDPQ 121
           GNSAGTVT FYL S G   DEIDFEFLG + G PY L TN++  G G +E QF   FDP 
Sbjct: 84  GNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGDKEQQFHLWFDPT 143

Query: 122 LIFTPTPFSGLLFISYRLY--VDGIPIRELKNKKEKGAPFPVRQPMRIYASLWNADSWAT 181
           + F       +++   R+   +DGIPIRE KN +  G PFP  QPMR+YASLW A+ WAT
Sbjct: 144 VDFHT---YCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLYASLWEAEHWAT 203

Query: 182 RGGAVKIDWTKAPFRAWYKNLSADGCLWSDGRSNCSSTSASWLSSTLDSVSQRHMKSAQT 241
           RGG  K DW+KAPF A+Y+N + D C+WS+G+S+CS+ S SW +  LD   +  +K AQ 
Sbjct: 204 RGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANS-SWFTQVLDFKGKNRVKWAQR 263

Query: 242 KYLFYDYCTDTNRFPLGLPLEC 261
           KY+ Y+YCTD  RFP G P EC
Sbjct: 264 KYMVYNYCTDKKRFPQGAPPEC 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022933964.13.0e-12382.64xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita moschata][more]
KAG6587762.13.0e-12382.64Xyloglucan endotransglucosylase/hydrolase protein 22, partial [Cucurbita argyros... [more]
XP_023003613.13.0e-12382.26xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita maxima][more]
XP_023531496.13.9e-12382.26xyloglucan endotransglucosylase/hydrolase protein 22-like [Cucurbita pepo subsp.... [more]
XP_038880549.14.3e-11477.44probable xyloglucan endotransglucosylase/hydrolase protein 23 [Benincasa hispida... [more]
Match NameE-valueIdentityDescription
Q388577.3e-8053.58Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=... [more]
Q389107.3e-8054.92Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis tha... [more]
Q389074.7e-7954.23Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis tha... [more]
O808039.8e-7752.08Probable xyloglucan endotransglucosylase/hydrolase protein 17 OS=Arabidopsis tha... [more]
Q9FI311.1e-7551.53Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1KX231.5e-12382.26Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1F1801.5e-12382.64Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5D3CL192.0e-10973.70Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BXV42.0e-10973.70Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC10349427... [more]
A0A6J1C4G16.1e-10670.96Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G25810.15.2e-8154.92xyloglucan endotransglycosylase 6 [more]
AT5G57560.15.2e-8153.58Xyloglucan endotransglucosylase/hydrolase family protein [more]
AT5G57550.13.4e-8054.23xyloglucan endotransglucosylase/hydrolase 25 [more]
AT1G65310.17.0e-7852.08xyloglucan endotransglucosylase/hydrolase 17 [more]
AT5G48070.17.7e-7751.53xyloglucan endotransglucosylase/hydrolase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.120.200coord: 1..260
e-value: 1.8E-80
score: 272.2
NoneNo IPR availablePANTHERPTHR31062:SF220XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASEcoord: 2..260
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 1..260
e-value: 5.7E-90
score: 299.3
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 10..189
e-value: 2.3E-40
score: 138.0
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 1..196
score: 19.650537
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 216..260
e-value: 3.5E-16
score: 58.8
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 2..260
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 12..260

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg010653.1Spg010653.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0071555 cell wall organization
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity