Spg010593 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg010593
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptiondisease resistance protein RGA2-like
Locationscaffold5: 13352433 .. 13355649 (-)
RNA-Seq ExpressionSpg010593
SyntenySpg010593
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACTTGCAGCTGGGCAAATTGATCGAGTATGGAATTTGGAGAAGGACCTCAAGAGATTGAGAAACCAGTTGGTTGTTTCTGAAGAGTTGTTGAGTCGTATCAACACAGGAGAGTGTAAAGAATCTCTGAAGCAGTGGGTGGAGGATCTTCACGAGATTGTTCTTGAAGTTGAGGATCTATTAGATGAGCTTGTTTACGAACATCTTCGACAAAAGGTGGAAAACGCAATTCTTTTCCTACTGTAGACAACTGTTTATTTGGTACTAGGATTGCAAATAAATTGAAAGATATTATGGAGCAGTTACGCTAGAATAACCGTGACTTAGACCTATTAAACTCTCTTCACAAGCCATCCACTGAAACACAAAGCATTCACGGCCAGCTTTTGGAGACAACTTCGCAGGCTGATGAAGGTGGAGTTGTGGGAAGGGTCGGATTCGGAAGTTTCAAGTTTGGTGGATCAAGTGATTGAGGCCAGCCAACGACAAGTTAATTCTATCCTACCCATTGTTGGTATGGGTGGATCAGGCAAAACAACTTTGGCCAAGTTGCTCTTCCATCACCACAGTATAAAAGCCCATTTTGATACGACTATATGGGTATGTGTGTCTGAACCTTTTGTGTTGGGAATTAGAGAGTGCAACCAAAGTCCCACATTGGCTAGATAAGGGGATGATCATGGGTTTATAAGGGAGACAAGTATCTCCATTGGTATGAGGCCTTTTGGGGTGAACCAAAAGCAAAGCCATGAAGGGTTTATGCCCAAAGTGGACAATATCATACCATTGTGGAGATCTATGTCGTGTCCTCGTCCCTTACAAGTGGTATTAGAGCCATGCCCCTGACGTAGCCGTTTCGTAAATTCCTCGGGTGCCGAACAAAGAATTTGTGAGCCTTGAAAAAATAGTCTTGGTGGAGATCCGTAATTAGGCTCTATTGGGTAGGCGTTTGGCCTAGATAAGAGCAAACTCCTTGGTGTGAAGCCTTGGTAAAGGAGTGTTTGACGGATGGATGACTATTTGAAGGGAGGCTCGTTCGTGGTACTTTGTTGAAGAAATAAAAGCAACACAGGAGATTCACTCCAACCAGATTGCACAATGCAGACAACAAATTTACAGAGAAAGTAAAGAAGAGGGAAGAAGGGAATGAACACCAACAGAATTATTAGCCATCAATTTTCAACAATTTGTCGTCAACAAGATTTTGCAAGCAATCTTACAGAATCTGGAGGATAATCCTAGTGATTGCAGCGACAATAGGGAGGCCTTACTTGGAAAGCTGAGGAGAAAGATGGATGACAAAAGATATTTTCTTGTGCTTGACGATGTTTGGAATGAGGATAAAATGAACTGGGAGAAGTTGAAGGAATGTTTAACGACGATTACTAAAAAATCGGGAAATAGTATCCTTATAACTACGAGGAGTACTGAAGGTGCCAATGTGATGAAACAAGAAGTTGATGCTCATCAATTATGCAGATTAACTAAAGATCAATGTTGGGCCTTATTCGTGAAAAGTGCAAATGCAAATGAAGTCCCAATGACTCCCGATTTGGAGCTTGTCCGAGAAGAGTTGGTTAAAAAGATTGGTGGGATTCCATTAGTTGCTAAAGTTTTGGGACGAGCAGTACAATTTCCAGGAGACCATGACCAATGGGTCACAATGTTAAGATGTGTTGTAACAACTCCATTAAAAAAGGATGATTTTGTTACATTCACATTCAAGTTAAGTGTGGATCGTCTACCAAAACCTTCAATAAAGCAATGTTTTGCTTACTGTTCAAATTTTCCCAAAGATTTTGAGTTTGACAAAGAGGAATTGATTCAAATGTGGATGGCACAAGGGTTTACTCAACCAGAAGAAGGAAACAACGAAACAATGGAAGAAATAGGAGACAGGTACTTCAATATCTTGTTGTCTCCTTGCTTATTTCAAGATATTGAATATGATGGAGAGAGAATTATAAGTTGCAAGATGCATGATCTTATACATGATATTGCATGTGATATTTCAAATAAGAAAATATTAATACAATTAGATCATAGTAAACCATCAAACTGGGAACTTTGGACGAAAGAGGATAAAAAATATGCAAGCAAGTTACCATACGGTTTTTTTTACGATGATTCAACTTGGGTTAGTCATGGATTATTGCAGAAGGTGATTCATGATAAGATCGTGGAATTTGTTGGTTTGCGGGTTTTGATTGTGGATTCAATGGCTGTCAATGAGTTACCTGATTCAATTTGCAAGTTGAAGCATTTGAGATATCTTGACATTTCACGTACAAAAATAAAGAAGATTCCAGAATCTATTGTTTTGCTTTATAATTTGCAAACACTGAGGTTTAGTCCCGGTTTGTACGAGCTTCCAGAAAATTTGAGAGAATTGGTTAGTTTACGACATTTGGTTTGTGACCGTTTGAAGCAAATGCCTGCATATTTGAGCCAATTGATTCAACTTCAAACGTTGCCTTATTTTAGAGTAGGATTTGAGAAGTGTCGTAAAATTACGGAACTTGGACCCATGAAAAACTTGAAAGGTTCTTTGATACTTGAGAATCTAGAACGAGTTGGAAGTAAAGAGGAAGCCAAGAATGCGAAATTGGTTGAAAAGGAGAACTTACACGAACTACGCCTTCGTTGGAGTTATCGTAGTGAAGTAAAGCATCACAATGATTTGGAAGTTTTGGAAGGCCTTCAACCACACAAAAGTCTACGGCATTTGGAAATTGATGGGCTTCTAGGTGGATGTTTTCCTAACAAAATATTTTCTGAGAATTTAGTAAGTATAAGGCTAGGTTATGGTAGAGATTGTGAAAAGCTACCTATGTTGGGACAATTAGCCAACTTAGAGAGACTTGAGATTCATTACTTTCCCAGGGTACATAGTATAGGCAATGAATTCTATGGAAACTACTCCAACCAAAGAACATTATTTCCCAAGTTAAAGTATTTAGATCTCAGCAAGATGATTGACCTAGTGCAGTGGGAAGAAGTGACAGATGCATCAAAAGTTGCAACTTTTCCTCGTCTTCAAGAGTTGCATATTTCATTCTGTCGAAGGTTATTGAAAATTCCAGATCTTTTTGGTTATTGTGATGAGAATGGTGAGAAGCACCTTAAATTTGTGGACATTTATAGGTGTGTCCAATTGAAGGAACTTCCAAATGGGCTACAATCTTGCGATTCCATTCAACGGTCGGAAATATAG

mRNA sequence

ATGAAACTTGCAGCTGGGCAAATTGATCGAGTATGGAATTTGGAGAAGGACCTCAAGAGATTGAGAAACCAGTTGGTTGTTTCTGAAGAGTTGTTGAGTCGTATCAACACAGGAGAGTGTAAAGAATCTCTGAAGCAGTGGGTGGAGGATCTTCACGAGATTGTTCTTGAAGTTGAGGATCTATTAGATGAGCTTGTTTACGAACATCTTCGACAAAAGGTGGAGTTGTGGGAAGGGTCGGATTCGGAAGTTTCAAGTTTGGTGGATCAAGTGATTGAGGCCAGCCAACGACAAGTTAATTCTATCCTACCCATTGTTGGTATGGGTGGATCAGGCAAAACAACTTTGGCCAAGTTGCTCTTCCATCACCACAGTATAAAAGCCCATTTTGATACGACTATATGGAATCTGGAGGATAATCCTAGTGATTGCAGCGACAATAGGGAGGCCTTACTTGGAAAGCTGAGGAGAAAGATGGATGACAAAAGATATTTTCTTGTGCTTGACGATGTTTGGAATGAGGATAAAATGAACTGGGAGAAGTTGAAGGAATGTTTAACGACGATTACTAAAAAATCGGGAAATAGTATCCTTATAACTACGAGGAGTACTGAAGGTGCCAATGTGATGAAACAAGAAGTTGATGCTCATCAATTATGCAGATTAACTAAAGATCAATGTTGGGCCTTATTCGTGAAAAGTGCAAATGCAAATGAAGTCCCAATGACTCCCGATTTGGAGCTTGTCCGAGAAGAGTTGGTTAAAAAGATTGGTGGGATTCCATTAGTTGCTAAAGTTTTGGGACGAGCAGTACAATTTCCAGGAGACCATGACCAATGGGTCACAATGTTAAGATGTGTTGTAACAACTCCATTAAAAAAGGATGATTTTGTTACATTCACATTCAAGTTAAGTGTGGATCGTCTACCAAAACCTTCAATAAAGCAATGTTTTGCTTACTGTTCAAATTTTCCCAAAGATTTTGAGTTTGACAAAGAGGAATTGATTCAAATGTGGATGGCACAAGGGTTTACTCAACCAGAAGAAGGAAACAACGAAACAATGGAAGAAATAGGAGACAGGTACTTCAATATCTTGTTGTCTCCTTGCTTATTTCAAGATATTGAATATGATGGAGAGAGAATTATAAGTTGCAAGATGCATGATCTTATACATGATATTGCATGTGATATTTCAAATAAGAAAATATTAATACAATTAGATCATAGTAAACCATCAAACTGGGAACTTTGGACGAAAGAGGATAAAAAATATGCAAGCAAGTTACCATACGGTTTTTTTTACGATGATTCAACTTGGGTTAGTCATGGATTATTGCAGAAGGTGATTCATGATAAGATCGTGGAATTTGTTGGTTTGCGGGTTTTGATTGTGGATTCAATGGCTGTCAATGAGTTACCTGATTCAATTTGCAAGTTGAAGCATTTGAGATATCTTGACATTTCACGTACAAAAATAAAGAAGATTCCAGAATCTATTGTTTTGCTTTATAATTTGCAAACACTGAGGTTTAGTCCCGGTTTGTACGAGCTTCCAGAAAATTTGAGAGAATTGGTTAGTTTACGACATTTGGTTTGTGACCGTTTGAAGCAAATGCCTGCATATTTGAGCCAATTGATTCAACTTCAAACGTTGCCTTATTTTAGAGTAGGATTTGAGAAGTGTCGTAAAATTACGGAACTTGGACCCATGAAAAACTTGAAAGGTTCTTTGATACTTGAGAATCTAGAACGAGTTGGAAGTAAAGAGGAAGCCAAGAATGCGAAATTGGTTGAAAAGGAGAACTTACACGAACTACGCCTTCGTTGGAGTTATCGTAGTGAAGTAAAGCATCACAATGATTTGGAAGTTTTGGAAGGCCTTCAACCACACAAAAGTCTACGGCATTTGGAAATTGATGGGCTTCTAGGTGGATGTTTTCCTAACAAAATATTTTCTGAGAATTTAGTAAGTATAAGGCTAGGTTATGGTAGAGATTGTGAAAAGCTACCTATGTTGGGACAATTAGCCAACTTAGAGAGACTTGAGATTCATTACTTTCCCAGGGTACATAGTATAGGCAATGAATTCTATGGAAACTACTCCAACCAAAGAACATTATTTCCCAAGTTAAAGTATTTAGATCTCAGCAAGATGATTGACCTAGTGCAGTGGGAAGAAGTGACAGATGCATCAAAAGTTGCAACTTTTCCTCGTCTTCAAGAGTTGCATATTTCATTCTGTCGAAGGTTATTGAAAATTCCAGATCTTTTTGGTTATTGTGATGAGAATGGTGAGAAGCACCTTAAATTTGTGGACATTTATAGGTGTGTCCAATTGAAGGAACTTCCAAATGGGCTACAATCTTGCGATTCCATTCAACGGTCGGAAATATAG

Coding sequence (CDS)

ATGAAACTTGCAGCTGGGCAAATTGATCGAGTATGGAATTTGGAGAAGGACCTCAAGAGATTGAGAAACCAGTTGGTTGTTTCTGAAGAGTTGTTGAGTCGTATCAACACAGGAGAGTGTAAAGAATCTCTGAAGCAGTGGGTGGAGGATCTTCACGAGATTGTTCTTGAAGTTGAGGATCTATTAGATGAGCTTGTTTACGAACATCTTCGACAAAAGGTGGAGTTGTGGGAAGGGTCGGATTCGGAAGTTTCAAGTTTGGTGGATCAAGTGATTGAGGCCAGCCAACGACAAGTTAATTCTATCCTACCCATTGTTGGTATGGGTGGATCAGGCAAAACAACTTTGGCCAAGTTGCTCTTCCATCACCACAGTATAAAAGCCCATTTTGATACGACTATATGGAATCTGGAGGATAATCCTAGTGATTGCAGCGACAATAGGGAGGCCTTACTTGGAAAGCTGAGGAGAAAGATGGATGACAAAAGATATTTTCTTGTGCTTGACGATGTTTGGAATGAGGATAAAATGAACTGGGAGAAGTTGAAGGAATGTTTAACGACGATTACTAAAAAATCGGGAAATAGTATCCTTATAACTACGAGGAGTACTGAAGGTGCCAATGTGATGAAACAAGAAGTTGATGCTCATCAATTATGCAGATTAACTAAAGATCAATGTTGGGCCTTATTCGTGAAAAGTGCAAATGCAAATGAAGTCCCAATGACTCCCGATTTGGAGCTTGTCCGAGAAGAGTTGGTTAAAAAGATTGGTGGGATTCCATTAGTTGCTAAAGTTTTGGGACGAGCAGTACAATTTCCAGGAGACCATGACCAATGGGTCACAATGTTAAGATGTGTTGTAACAACTCCATTAAAAAAGGATGATTTTGTTACATTCACATTCAAGTTAAGTGTGGATCGTCTACCAAAACCTTCAATAAAGCAATGTTTTGCTTACTGTTCAAATTTTCCCAAAGATTTTGAGTTTGACAAAGAGGAATTGATTCAAATGTGGATGGCACAAGGGTTTACTCAACCAGAAGAAGGAAACAACGAAACAATGGAAGAAATAGGAGACAGGTACTTCAATATCTTGTTGTCTCCTTGCTTATTTCAAGATATTGAATATGATGGAGAGAGAATTATAAGTTGCAAGATGCATGATCTTATACATGATATTGCATGTGATATTTCAAATAAGAAAATATTAATACAATTAGATCATAGTAAACCATCAAACTGGGAACTTTGGACGAAAGAGGATAAAAAATATGCAAGCAAGTTACCATACGGTTTTTTTTACGATGATTCAACTTGGGTTAGTCATGGATTATTGCAGAAGGTGATTCATGATAAGATCGTGGAATTTGTTGGTTTGCGGGTTTTGATTGTGGATTCAATGGCTGTCAATGAGTTACCTGATTCAATTTGCAAGTTGAAGCATTTGAGATATCTTGACATTTCACGTACAAAAATAAAGAAGATTCCAGAATCTATTGTTTTGCTTTATAATTTGCAAACACTGAGGTTTAGTCCCGGTTTGTACGAGCTTCCAGAAAATTTGAGAGAATTGGTTAGTTTACGACATTTGGTTTGTGACCGTTTGAAGCAAATGCCTGCATATTTGAGCCAATTGATTCAACTTCAAACGTTGCCTTATTTTAGAGTAGGATTTGAGAAGTGTCGTAAAATTACGGAACTTGGACCCATGAAAAACTTGAAAGGTTCTTTGATACTTGAGAATCTAGAACGAGTTGGAAGTAAAGAGGAAGCCAAGAATGCGAAATTGGTTGAAAAGGAGAACTTACACGAACTACGCCTTCGTTGGAGTTATCGTAGTGAAGTAAAGCATCACAATGATTTGGAAGTTTTGGAAGGCCTTCAACCACACAAAAGTCTACGGCATTTGGAAATTGATGGGCTTCTAGGTGGATGTTTTCCTAACAAAATATTTTCTGAGAATTTAGTAAGTATAAGGCTAGGTTATGGTAGAGATTGTGAAAAGCTACCTATGTTGGGACAATTAGCCAACTTAGAGAGACTTGAGATTCATTACTTTCCCAGGGTACATAGTATAGGCAATGAATTCTATGGAAACTACTCCAACCAAAGAACATTATTTCCCAAGTTAAAGTATTTAGATCTCAGCAAGATGATTGACCTAGTGCAGTGGGAAGAAGTGACAGATGCATCAAAAGTTGCAACTTTTCCTCGTCTTCAAGAGTTGCATATTTCATTCTGTCGAAGGTTATTGAAAATTCCAGATCTTTTTGGTTATTGTGATGAGAATGGTGAGAAGCACCTTAAATTTGTGGACATTTATAGGTGTGTCCAATTGAAGGAACTTCCAAATGGGCTACAATCTTGCGATTCCATTCAACGGTCGGAAATATAG

Protein sequence

MKLAAGQIDRVWNLEKDLKRLRNQLVVSEELLSRINTGECKESLKQWVEDLHEIVLEVEDLLDELVYEHLRQKVELWEGSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIWNLEDNPSDCSDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNWEKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANEVPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTPLKKDDFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQPEEGNNETMEEIGDRYFNILLSPCLFQDIEYDGERIISCKMHDLIHDIACDISNKKILIQLDHSKPSNWELWTKEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDSICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRFSPGLYELPENLRELVSLRHLVCDRLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKEEAKNAKLVEKENLHELRLRWSYRSEVKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCFPNKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYSNQRTLFPKLKYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFCRRLLKIPDLFGYCDENGEKHLKFVDIYRCVQLKELPNGLQSCDSIQRSEI
Homology
BLAST of Spg010593 vs. NCBI nr
Match: XP_011650061.1 (putative disease resistance protein RGA3 [Cucumis sativus] >KGN63368.1 hypothetical protein Csa_022194 [Cucumis sativus])

HSP 1 Score: 806.2 bits (2081), Expect = 2.5e-229
Identity = 461/889 (51.86%), Postives = 583/889 (65.58%), Query Frame = 0

Query: 1   MKLAAGQIDRVWNLEKDLKRLRNQLVVSEELLSRINTGE----CKESL-KQWVEDLHEIV 60
           + L A QI     ++  L++L+ +LV S++++S I T        +SL  QWV DL  IV
Sbjct: 18  LTLVAEQIILAREVKDVLQQLQKELVESQKIVSAITTQRQNHYSPDSLVTQWVNDLQLIV 77

Query: 61  LEVEDLLDELVYEHLRQKV----------------------------------------- 120
            E +DLLD  VYEHL+Q+V                                         
Sbjct: 78  HEADDLLDLFVYEHLQQRVNPSAHGKIIKKVPHFLCASARTKKMKEIIALLNKHCTKLPH 137

Query: 121 ---------------------------ELWEGSDSEVSSLVDQVIEASQRQVNSILPIVG 180
                                      +   G + EV ++VD+VI+AS++++NSILP+ G
Sbjct: 138 LLQLEPTPSNIAETEVAQIQETVSKPEDYVVGRNREVETIVDRVIDASKQELNSILPVFG 197

Query: 181 MGGSGKTTLAKLLFHHHSIKAHFDTTIW------------------NLEDNPSDCSDNRE 240
           MGG GKTTLAK +F+H  IK HF  TIW                   +E + SDCS+NRE
Sbjct: 198 MGGLGKTTLAKSVFNHDRIKNHFGITIWIYVSQPFVINNILQAILQKVEVHSSDCSNNRE 257

Query: 241 ALLGKLRRKMDDKRYFLVLDDVWNEDKMNWEKLKECLTTITKKSGNSILITTRSTEGANV 300
           ALL KL   M +K YFLVLDDVWNE+KM WEKLKECL +IT  SGNSIL+TTRS+  A +
Sbjct: 258 ALLEKLTENMGEKTYFLVLDDVWNENKMLWEKLKECLMSITHMSGNSILVTTRSSGIAKM 317

Query: 301 MKQEVDAHQLCRLTKDQCWALFVKSANANEVPMTPDLELVREELVKKIGGIPLVAKVLGR 360
           M++ + +H+L +L+ DQCW++F   ANA +VPMT +LE V++E  K+IGG+PL+AKVLG 
Sbjct: 318 MEENIGSHELRKLSDDQCWSIFRNFANAKDVPMTSNLEFVQKEFDKRIGGLPLIAKVLGA 377

Query: 361 AVQFPGDHDQWVTMLRCVVTTPLKKDDFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFE 420
           AV F GDHDQWV  ++ V+TTP+K+++FV FT KLSVDRLP  S+KQCFAYCSNF K  E
Sbjct: 378 AVPFSGDHDQWVANIKSVLTTPIKEEEFVKFTLKLSVDRLPNASVKQCFAYCSNFSKGCE 437

Query: 421 FDKEELIQMWMAQGFTQPEEGNNETMEEIGDRYFNILLSPCLFQDIEYDGERII-SCKMH 480
           FDK+++I+MWMAQGFTQP+E NNETME+ G+RYFNILLS CLFQD+  +   II   +MH
Sbjct: 438 FDKKQVIRMWMAQGFTQPDERNNETMEDTGERYFNILLSFCLFQDVVKNERGIIEKVRMH 497

Query: 481 DLIHDIACDISNKKILIQLDHSKPSNWELWTKEDKKYASKLPYGFFYDDSTWVSHGLLQK 540
           DLIHDIAC +SN K L ++DH   SNW+ WTK+DK   SKL    FYD            
Sbjct: 498 DLIHDIACQVSNDKKL-RIDHIISSNWKDWTKDDKILVSKLRTINFYDRH--------HV 557

Query: 541 VIHDKIVEFVGLRVLIVDSMAVNELPDSICKLKHLRYLDISRT-KIKKIPESIVLLYNLQ 600
           V+ DKI +F GLRVL +++  V ELP+SI KLKHLRYLDIS    IKK+PESIVLLYNLQ
Sbjct: 558 VVQDKIGDFTGLRVLTIENYIVEELPNSIFKLKHLRYLDISYCYSIKKLPESIVLLYNLQ 617

Query: 601 TLRF---SPGLYELPENLRELVSLRHLVCDRL-KQMPAYLSQLIQLQTLPYFRVGFEKCR 660
           TLRF   S G   LP+N+ +++SLRHL    + KQM  YLSQLIQL+TLP F VGFEK  
Sbjct: 618 TLRFHLLSKGF--LPKNVGQMISLRHLEFSSIDKQMSPYLSQLIQLETLPKFAVGFEKGC 677

Query: 661 KITELGPMKNLKGSLILENLERVGSKEEAKNAKLVEKENLHELRLRWS--YRSEVKHHND 720
           KITELG ++NLKG L L+ LE V SKEEA+ AKLVEKENL E+   W+   + +V++ ND
Sbjct: 678 KITELGVLRNLKGLLKLQRLEHVESKEEAETAKLVEKENLEEVHFVWTKERKRKVENKND 737

Query: 721 LEVLEGLQPHKSLRHLEIDGLLGGCFPNKIFSENLVSIRLGYGRDCEKLPMLGQLANLER 780
           LEVLEGLQP K++ +L I   LGGC PN+ F ENLV I L    +CEKLP LGQL NLE 
Sbjct: 738 LEVLEGLQPPKNVEYLRIKYFLGGCLPNQTFVENLVKIELRDCGNCEKLPRLGQLGNLEI 797

Query: 781 LEIHYFPRVHSIGNEFYGNYS-NQRTLFPKLKYLDLSKMIDLVQWEEVTDASKVATFPRL 790
           L+I +F RV SIGNEFYGN S NQR+LFP+LK L + +M  + +WEEV   S V  FPRL
Sbjct: 798 LDISWFERVKSIGNEFYGNSSNNQRSLFPRLKELYVDEMRRIGEWEEV--GSNVKAFPRL 857

BLAST of Spg010593 vs. NCBI nr
Match: XP_022944234.1 (disease resistance protein RGA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 786.9 bits (2031), Expect = 1.6e-223
Identity = 447/874 (51.14%), Postives = 568/874 (64.99%), Query Frame = 0

Query: 1   MKLAAGQIDRVWNLEKDLKRLRNQLVVSEELLSRINTGEC-KESLKQWVEDLHEIVLEVE 60
           ++LAA +I   W LEK+L  L+  L+ SE  L  INT +   +S++ WVE LH +V E +
Sbjct: 18  VRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHNDSVRLWVEKLHHLVHEAD 77

Query: 61  DLLDELVYEHLRQKVELWE----------------------------------------- 120
           DL+D LVYEHLR KV+  +                                         
Sbjct: 78  DLIDNLVYEHLRHKVQKNDKMNKLNKHDREATRLGLVGDEPIQTLLQSDVSQIRETVSEV 137

Query: 121 ------GSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDT 180
                 G D EVSS+V QVI+A  ++V SILPIVGMGG GKTTLA+L+F+H  IK HFD 
Sbjct: 138 DDFEVVGRDVEVSSIVKQVIDAHNQRVTSILPIVGMGGLGKTTLARLIFNHEMIKQHFDE 197

Query: 181 TIWNLEDNPSD-----------------CSDNREALLGKLRRKMDDKRYFLVLDDVWNED 240
           TIW     P D                 C  NRE LL +L+++M  KRYFLVLDDVWNED
Sbjct: 198 TIWVCVSIPFDINKILGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNED 257

Query: 241 KMNWEKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSA 300
            + WE+LK  L  IT KSGNSIL+TTR+ E   +M++ +  H + +L+ +QCW+LF++SA
Sbjct: 258 SLLWEQLKNFLMKITSKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESA 317

Query: 301 NANEVPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTP--LK 360
           NAN +P T +LE VREELVKK GGIPLVA+VLG AV+F  D+D WV++L  V+ +P  L+
Sbjct: 318 NANGLPTTSNLEAVREELVKKFGGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQ 377

Query: 361 KDDFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQPEEGNN- 420
             +F     KLSVD LP  S+K CFAYCSNFPKD+EF K+ LIQMW+AQGF Q +EG + 
Sbjct: 378 GKNFCLPILKLSVDYLPS-SLKPCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSC 437

Query: 421 -ETMEEIGDRYFNILLSPCLFQDIEYDGE-RIISCKMHDLIHDIACDISNKKILIQLDHS 480
            +T+EE G+RYF ILLS CLFQD+  D   RIISCKMHDLIHD+ACD+SN +  + LD  
Sbjct: 438 YQTVEEEGERYFKILLSRCLFQDVVKDARGRIISCKMHDLIHDMACDVSNDQKSL-LDSC 497

Query: 481 KPSNWELWTKEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAV 540
             S+ ELW    K   SKL     ++    + H  ++K +HDKI  FV LRVLI++S ++
Sbjct: 498 TSSDEELWRNRTKNIGSKLRMIACHEG---MPH-RIEKAVHDKIGSFVCLRVLIMNSRSI 557

Query: 541 NELPDSICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRF-SPGLYELPENLRELVSLR 600
            +LP SI +LKHLRY+DIS   I+K+P+SIVLLYNL+TLR  S  + ELP++LR LV+LR
Sbjct: 558 YKLPYSIGELKHLRYVDISGCLIQKLPKSIVLLYNLKTLRIGSSPIEELPKDLRRLVNLR 617

Query: 601 HLVCDRL-----KQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLE 660
           HL    +     K+MP +LSQLI LQTL  F VGFEK  +I ELGP+KNLKGSL + NLE
Sbjct: 618 HLEFSEIYFVDPKKMPPHLSQLIHLQTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLE 677

Query: 661 RVGSKEEAKNAKLVEKENLHELRLRWSYRSEVK----HHNDLEVLEGLQPHKSLRHLEID 720
           RV S+EEAK+A  +EKE L EL L W + +E +    +HN  +VLEGLQPHK L  L I 
Sbjct: 678 RVKSREEAKSANFMEKEKLRELVLEWGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIH 737

Query: 721 GLLGGCFPNKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGN 780
             +G C  N IF +NLV+I L   R+C +LPMLG L +LE L +     V SIG+EFYGN
Sbjct: 738 SFVGECLANNIFVDNLVAISLVDCRNCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYGN 797

Query: 781 YSNQRTLFPKLKYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFCRRLLKIPDLFGY 795
           +SNQR LFPK+K   L ++  L QWEEVT  + V TFP+LQ L IS C RLL I DLFG 
Sbjct: 798 HSNQRALFPKVKIFKLIEVERLEQWEEVTVGAHVTTFPQLQVLEISKCPRLLSISDLFGC 857

BLAST of Spg010593 vs. NCBI nr
Match: XP_022148515.1 (disease resistance protein RGA2-like [Momordica charantia])

HSP 1 Score: 747.7 bits (1929), Expect = 1.1e-211
Identity = 412/747 (55.15%), Postives = 521/747 (69.75%), Query Frame = 0

Query: 79  GSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHF-DTTIW-- 138
           G D E+SS+VD+VI+A+ R V SILPIVG+GG GKTTLAKL+ +H  IK HF + TIW  
Sbjct: 169 GRDVELSSIVDRVIDATNRHVTSILPIVGVGGLGKTTLAKLVINHERIKHHFNNNTIWVC 228

Query: 139 ----------------NLEDNPSDCSDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNW 198
                            L+D PS     +E LLG+L ++M  K+YFLVLDDVWNE+++ W
Sbjct: 229 VSQPFNVNKILGTILETLKDTPSGLEYKKEVLLGQLYKEMRGKKYFLVLDDVWNENRLLW 288

Query: 199 EKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANE 258
           ++LK CL  IT+ S N IL+TTRS E A +M + + +H L +L+ DQCW+LF +SANAN 
Sbjct: 289 DELKNCLMKITENSENGILVTTRSAEVAKIM-ETLPSHHLGKLSDDQCWSLFQESANANG 348

Query: 259 VPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTPLKKDDFVT 318
           + MT  L++VREELVKKIGG+PL+AKV G A Q+ GD D+WV  L+ +V   L+ ++  T
Sbjct: 349 LSMTSSLKVVREELVKKIGGLPLIAKVWGSAEQYLGDPDKWVINLKSLVCNSLQNENSAT 408

Query: 319 FTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQ-PEEGNNETMEEI 378
              KLSVDRLP+ S+KQCFAYCSNFPKDFEF KE+LIQMW+AQGF   P+  +NET+E+ 
Sbjct: 409 SALKLSVDRLPRHSLKQCFAYCSNFPKDFEFTKEQLIQMWIAQGFIHLPKGRSNETIEDE 468

Query: 379 GDRYFNILLSPCLFQDIEYDGERII-SCKMHDLIHDIACDISNKKILIQLDHSKPS-NWE 438
           GDR+F ILL  CLFQD+  D   II SCKMHDLIHDIACD+SN     +L +S  S + E
Sbjct: 469 GDRHFKILLRRCLFQDVVQDDRGIIVSCKMHDLIHDIACDVSNDH---KLQYSNNSLDGE 528

Query: 439 LWTKEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDS 498
           +WT E+KK ASKL    + +D   +   +++KVIHDK++ FV LRVL ++S +++ELPDS
Sbjct: 529 VWTDENKKIASKLRTIIYCEDQ--MMPPIMEKVIHDKVMNFVCLRVLKMNSRSISELPDS 588

Query: 499 ICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRFSPGLY-ELPENLRELVSLRHLV--- 558
           I +LKHLRYLDIS   I ++P+SI LLYNLQTL     L  +LP+N+ +LV+L+HL    
Sbjct: 589 IGELKHLRYLDISGCSITQLPKSIALLYNLQTLNMGGLLIKDLPKNIIDLVNLKHLQFYP 648

Query: 559 --CDRLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKE 618
              D  K MPAY  +L QLQTL YF VGFE   KI+ELG +KNLKGSL L  LE+V SKE
Sbjct: 649 YHFDTYK-MPAYFGKLTQLQTLSYFVVGFENGHKISELGLLKNLKGSLNLCFLEKVESKE 708

Query: 619 EAKNAKLVEKENLHELRLRWS---YRSEVKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCF 678
           EA+ AKLVEKENL EL   WS    R +   + D E+LEGLQPHK+LR L I+       
Sbjct: 709 EARAAKLVEKENLDELEFEWSQSHLRKDNDSYKDFEILEGLQPHKNLRSLSIERSAIKSL 768

Query: 679 PNKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYSNQRTL 738
           PN IF ENL  I L   ++CE+LPMLGQL NLERLE++    V SI NEFYGN  NQRTL
Sbjct: 769 PNNIFVENLAVISLRDCQNCERLPMLGQLRNLERLEVYGLHSVQSISNEFYGNDCNQRTL 828

Query: 739 FPKLKYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFCRRLLKIPDLFGYCDENGEK 795
           FPKL+   LS M +L QW+EVT AS + TFP L+ L+I  C +L+ IPD+FGYCDENG +
Sbjct: 829 FPKLRAFLLSDMENLEQWKEVTVASNLTTFPCLESLNIWRCPKLMNIPDVFGYCDENGVE 888

BLAST of Spg010593 vs. NCBI nr
Match: XP_022158717.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 716.8 bits (1849), Expect = 2.0e-202
Identity = 405/720 (56.25%), Postives = 498/720 (69.17%), Query Frame = 0

Query: 79  GSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIWNLE 138
           G D EVS +V+Q+I+A+   V SILPIVGMGG GKTT+AK++F+H  IK HFD TIW   
Sbjct: 169 GRDVEVSKIVNQLIDATNTHVTSILPIVGMGGLGKTTMAKVVFNHERIKTHFDKTIWVCV 228

Query: 139 DNP-----------------SDCSDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNWEK 198
             P                 S   DN+EALL +L+++M  KRYFLVLDDVWNE    W++
Sbjct: 229 SEPFLVNKILGAILGTLRGTSSGLDNKEALLHELKKEMHGKRYFLVLDDVWNESHSLWDE 288

Query: 199 LKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANEVP 258
           LK CL  IT++S NSIL+TTRS E A +M + +  H L +L+ DQCW+LF++SAN N + 
Sbjct: 289 LKNCLMRITERSENSILVTTRSAEVAKIM-ETLPNHHLSKLSDDQCWSLFLESANVNGLS 348

Query: 259 MTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTT--PLKKDDFVT 318
           +T  L++V +ELVKKIGGIPLVA+VLGRAV+F GD++ WVT L  ++ T   L+++ FV 
Sbjct: 349 ITSSLKVV-QELVKKIGGIPLVARVLGRAVKFEGDYETWVTTLESIIRTRLQLQEESFVL 408

Query: 319 FTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQ-PEEGNNETMEEI 378
              KLSVDRLP  S+KQCF+YCSNFPKDF F+KE LI+MWMAQGF Q  E  +N+TME+ 
Sbjct: 409 SILKLSVDRLPLSSLKQCFSYCSNFPKDFVFEKEPLIRMWMAQGFIQLQERRSNKTMEDE 468

Query: 379 GDRYFNILLSPCLFQDIEYDGE-RIISCKMHDLIHDIACDISNKKILIQLDHSKPSNWEL 438
           GD YF ILLS CLFQDI  DG+ RIIS KMHDLIHDIAC+ISN K L  LD     + E 
Sbjct: 469 GDTYFKILLSRCLFQDIIKDGKGRIISFKMHDLIHDIACNISNDKKL-HLDPCNLLDNEH 528

Query: 439 WTKE-DKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDS 498
           W  E  +K ASKL       ++        ++VI DK+  F+ LRVLI++     +LPDS
Sbjct: 529 WRNEISEKVASKLRTVICRRETL----HRREQVIDDKMSNFIWLRVLIINFWNAKKLPDS 588

Query: 499 ICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRF-SPGLYELPENLRELVSLRHLV--- 558
           I KLKHLRYLDIS   + ++PESIV LY+LQTLR     + +LP+NL +LVSLRHL    
Sbjct: 589 IGKLKHLRYLDISGCSMDELPESIVSLYHLQTLRIEGTMIVDLPKNLGKLVSLRHLEFSF 648

Query: 559 ---CDRLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSK 618
                ++ QMP++LSQL+QLQTL  F VGFEK RKITELGP+KNLKGSL L NLER+ SK
Sbjct: 649 FLGIKKILQMPSHLSQLVQLQTLSTFIVGFEKGRKITELGPLKNLKGSLNLSNLERIKSK 708

Query: 619 EEAKNAKLVEKENLHELRLRWSYRS--EVKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCF 678
           EEA  AKL EKENLHEL   W+  S  E  +HNDL+VLEGLQPHKSL+ L I   LG C 
Sbjct: 709 EEAMFAKLGEKENLHELTFTWTCSSEREYNNHNDLDVLEGLQPHKSLQSLSIQSFLGECL 768

Query: 679 PNKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYSNQRTL 738
           PNKIF ENLV+I L    +CE+LPMLGQL NLE L I     V SIGNEFYGN S+QRT 
Sbjct: 769 PNKIFVENLVAISLVDCENCERLPMLGQLTNLEELCISGLHSVRSIGNEFYGNDSDQRTF 828

Query: 739 FPKLKYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFCRRLLKIPDLFGYCDENGEK 768
           FPKLK   L+KM +L QWEEVT A  +  FP ++   IS C +LLK+P +FG  D+N  K
Sbjct: 829 FPKLKKFTLNKMSNLEQWEEVTLAPNLTVFPCIEMWKISECFKLLKVPKIFGSWDKNHVK 881

BLAST of Spg010593 vs. NCBI nr
Match: XP_038900462.1 (disease resistance protein RGA2-like [Benincasa hispida])

HSP 1 Score: 705.7 bits (1820), Expect = 4.6e-199
Identity = 416/909 (45.76%), Postives = 550/909 (60.51%), Query Frame = 0

Query: 1   MKLAAGQIDRVWNLEKDLKRLRNQLVVSEELLSRINTGECK-ESLKQWVEDLHEIVLEVE 60
           M +A+ QI   W  ++DL  LR  L  ++ +L+ INT +     +K WVE L  ++ + +
Sbjct: 20  MAVASEQISLAWGFKEDLLELREALRYAKGILNDINTTKLHYNPVKDWVEKLQHLIYQAD 79

Query: 61  DLLDELVYEHLRQKVELWE----------------------------------------- 120
           DLLDE+VYE LR++V+  +                                         
Sbjct: 80  DLLDEIVYEDLRREVQTEKMKMVCNFISPSNNTFMFRSKMAKKMKSVKNLLQKHYSIEIG 139

Query: 121 -----------------------------------GSDSEVSSLVDQVIE--ASQRQVNS 180
                                              G D EV S+V QVIE  + +++  S
Sbjct: 140 PLQLVCNRSVESESSNVISQSRETISELDDYEEVVGRDVEVESIVKQVIEDGSKEQEAIS 199

Query: 181 ILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIWNLEDNP------------------SD 240
           ILPIVGMGG GKTTLAK++F+H  IK HFD TIW     P                  S 
Sbjct: 200 ILPIVGMGGLGKTTLAKMVFNHQMIKQHFDKTIWVCVSEPFNVNKILGAILQSVTCTTSK 259

Query: 241 CSDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNWEKLKECLTTITKKSGNSILITTRS 300
             DN++ LLG+L+ +M  KRYFLVLDDVWNE+++ W +LK+CL  IT+KS +S+++TTRS
Sbjct: 260 GGDNKQVLLGELQNEMHGKRYFLVLDDVWNENEILWAELKKCLIKITRKSKSSVVVTTRS 319

Query: 301 TEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANEVPMTPDLELVREELVKKIGGIPLV 360
            + A +M + + +H LC+L+ + CW+LF +SA+A  +PMT  LE++R+ELVKKIGG+PLV
Sbjct: 320 ADAAKIM-ETLPSHHLCKLSDNHCWSLFKESASAYGLPMTSSLEVIRKELVKKIGGVPLV 379

Query: 361 AKVLGRAVQFPGDHDQWVTMLRCVVTTPLKKDDFVTFTFKLSVDRLPKPSIKQCFAYCSN 420
           A+VLGRAV+F G  ++ + ML  +V +  + ++F     KLSVDRLP  S+KQCFAYCSN
Sbjct: 380 ARVLGRAVKFEGGDERQMKMLESIVRSTFQDENFTLSILKLSVDRLPFSSLKQCFAYCSN 439

Query: 421 FPKDFEFDKEELIQMWMAQGFTQPEEGNNET-MEEIGDRYFNILLSPCLFQDIEYDGE-R 480
           FPKDF F KE+L+QMWMAQGF Q + G  +   + +GD YF+ILLS CLFQD + D E R
Sbjct: 440 FPKDFVFQKEQLVQMWMAQGFIQAQGGRIDVRTKNVGDEYFSILLSRCLFQDAKKDSEGR 499

Query: 481 IISCKMHDLIHDIACDISNKKILIQLDHSKPSNWELWTKEDKKYASKLPYGFFYDDSTWV 540
           IISCKMHDLIHDIAC++S+             N EL +    +  SK+     Y+++   
Sbjct: 500 IISCKMHDLIHDIACEVSS-----------DGNLELNSSNLLENVSKVRTMISYEETLPK 559

Query: 541 SHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDSICKLKHLRYLDISRTKIKKIPESIV 600
                 K++ DKI +F+ LRVLIV+S ++ +L +S+ KLKHLRYL+IS  +I ++P SIV
Sbjct: 560 EK---MKLVDDKITKFLPLRVLIVNSWSIYKLSNSVGKLKHLRYLEISDPEIVELPPSIV 619

Query: 601 LLYNLQTLRFSPGLY-ELPENLRELVSLRHLVCDR----LKQMPAYLSQLIQLQTLPYFR 660
           LLYNLQTLR +  +  +LP+NLRELVSLRHL         K+MP +LS+L QLQTLP F 
Sbjct: 620 LLYNLQTLRIANSMIRDLPKNLRELVSLRHLDFSMKNLVAKKMPPHLSRLTQLQTLPIFV 679

Query: 661 VGFEKCRKITELGPMKNLKGSLILENLERVGSKEEAKNAKLVEKENLHELRLRWS-YRSE 720
           V  EK   I ELGP+ NLK SL L  LE+V SKE+A    L  KE+L  L   WS  R+ 
Sbjct: 680 VAVEKGCDIAELGPLPNLKDSLQLFCLEKVQSKEKANEGSLEGKEHLDGLAFNWSKQRNT 739

Query: 721 VKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCFPNKIFSENLVSIRLGYGRDCEKLPMLGQ 780
               +DLEVLEGLQP K+L  L+I    G C PNKIF ENL +I L   ++CE+ PMLGQ
Sbjct: 740 ATCCSDLEVLEGLQPPKNLGSLKIHNFAGKCLPNKIFVENLRAIGLWDCKNCERFPMLGQ 799

Query: 781 LANLERLEIHYFPRVHSIGNEFYGNY-SNQRTLFPKLKYLDLSKMIDLVQWEEVTD---- 799
           L NL+ LEI+    V SIG EFYGNY SNQRT F KL+   L  MI+L  WEE+T     
Sbjct: 800 LNNLKELEIYGLDSVQSIGKEFYGNYESNQRTFFSKLERFILKGMINLEHWEEITTHDDY 859

BLAST of Spg010593 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.9e-91
Identity = 252/772 (32.64%), Postives = 386/772 (50.00%), Query Frame = 0

Query: 79  GSDSEVSSLVDQVI-EASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIW-- 138
           G D E   +V  +I   S  Q  S+LPI+GMGG GKTTLA+++F+   +  HF + IW  
Sbjct: 153 GRDKEKDEIVKILINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWIC 212

Query: 139 ----------------NLEDNPSDCSDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNW 198
                           ++E  P     +   L  KL+  ++ KRY LVLDDVWNED+  W
Sbjct: 213 VSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKW 272

Query: 199 EKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANE 258
             L+  L      SG S+L TTR  +  ++M   +  ++L  L+++ CW LF++ A  ++
Sbjct: 273 ANLRAVLK--VGASGASVLTTTRLEKVGSIM-GTLQPYELSNLSQEDCWLLFMQRAFGHQ 332

Query: 259 VPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTPLKKDDFVT 318
             + P+L  + +E+VKK GG+PL AK LG  + F  +   W  +    +    + +  + 
Sbjct: 333 EEINPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSIL 392

Query: 319 FTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQPEEGNNETMEEIG 378
              +LS  +LP   +KQCFAYC+ FPKD + +KE+LI +WMA GF    +GN E +E++G
Sbjct: 393 PALRLSYHQLPL-DLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLL-SKGNME-LEDVG 452

Query: 379 DRYFNILLSPCLFQDIEY-DGERIISCKMHDLIHDIACDISNKKILIQLDHSKPSNWELW 438
           D  +  L     FQ+IE  DG+     KMHDLIHD+A  + +        ++  SN    
Sbjct: 453 DEVWKELYLRSFFQEIEVKDGKTYF--KMHDLIHDLATSLFSA-------NTSSSNIREI 512

Query: 439 TKEDKKYASKLPYG---FFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPD 498
            K    +   + +    FFY                  + +F+ LRVL +     N+LP 
Sbjct: 513 NKHSYTHMMSIGFAEVVFFY--------------TLPPLEKFISLRVLNLGDSTFNKLPS 572

Query: 499 SICKLKHLRYLDISRTKIKKIPESIVLLYNLQT--LRFSPGLYELPENLRELVSLRHLVC 558
           SI  L HLRYL++  + ++ +P+ +  L NLQT  L++   L  LP+   +L SLR+L+ 
Sbjct: 573 SIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLL 632

Query: 559 D---RLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKE 618
           D    L  MP  +  L  L+TL  F VG +K  ++ ELG + NL GS+ + +LERV + +
Sbjct: 633 DGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERVKNDK 692

Query: 619 EAKNAKLVEKENLHELRLRW-SYRSEVKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCFP- 678
           +AK A L  K NLH L + W ++   +    +++VLE L+PH +L  L+I G  G   P 
Sbjct: 693 DAKEANLSAKGNLHSLSMSWNNFGPHIYESEEVKVLEALKPHSNLTSLKIYGFRGIHLPE 752

Query: 679 --NKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHY----FPRVHSIGNEFYGNYS 738
             N    +N+VSI +   R+C  LP  G L  LE LE+H+       V  +  + +  + 
Sbjct: 753 WMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFP 812

Query: 739 NQRTLFPKLKYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFCRRLL---------- 798
             R  FP L+ LD+    D    + +        FP L+E+ I  C  L           
Sbjct: 813 T-RIRFPSLRKLDI---WDFGSLKGLLKKEGEEQFPVLEEMIIHECPFLTLSSNLRALTS 872

BLAST of Spg010593 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 334.7 bits (857), Expect = 2.8e-90
Identity = 267/837 (31.90%), Postives = 409/837 (48.86%), Query Frame = 0

Query: 6   GQIDRVWNLEKDLKRLRNQLVVSEELLSRINTGECK-ESLKQWVEDLHEIVLEVEDLLDE 65
           G++  V+  EK+ K+L +   + + +L      + K +++K W++ L+    EV+D+LD+
Sbjct: 19  GELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDD 78

Query: 66  LVYEHLRQK----------------------VELWEGSDSEVSS-----LVDQVIE--AS 125
              E  R K                       E+ E  D+         L +++IE  A+
Sbjct: 79  CKTEAARFKQAVLGRYHPRTITFCYKVGKRMKEMMEKLDAIAEERRNFHLDERIIERQAA 138

Query: 126 QRQVN--------------------------------SILPIVGMGGSGKTTLAKLLFHH 185
           +RQ                                   +LPI+GMGG GKTTLA+++F+ 
Sbjct: 139 RRQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFND 198

Query: 186 HSIKAHFDTTIWNLEDNPSDCSDNREALL-----------------GKLRRKMDDKRYFL 245
             I  HF+  IW    +  D     +A++                  KL+  ++ KRYFL
Sbjct: 199 QRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFL 258

Query: 246 VLDDVWNEDKMNWEKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQ 305
           VLDDVWNED+  W+ L+  L      SG SILITTR  +  ++M   +  +QL  L+++ 
Sbjct: 259 VLDDVWNEDQEKWDNLRAVLK--IGASGASILITTRLEKIGSIM-GTLQLYQLSNLSQED 318

Query: 306 CWALFVKSANANEVPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRC 365
           CW LF + A  ++   +P L  + +E+VKK GG+PL AK LG  ++F  +  +W  +   
Sbjct: 319 CWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDS 378

Query: 366 VVTTPLKKDDFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQ 425
            +    + ++ V    +LS   LP   ++QCFAYC+ FPKD + +KE LI +WMA  F  
Sbjct: 379 EIWNLPQDENSVLPALRLSYHHLPL-DLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLL 438

Query: 426 PEEGNNETMEEIGDRYFNILLSPCLFQDIEYDGERIISCKMHDLIHDIACDISNKKILIQ 485
             +GN E +E++G+  +N L     FQ+IE    +    KMHDLIHD+A        +  
Sbjct: 439 -SKGNME-LEDVGNEVWNELYLRSFFQEIEVKSGKTY-FKMHDLIHDLATS------MFS 498

Query: 486 LDHSKPSNWELWTKEDK-------KYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVG 545
              S  S  ++  K+D+        Y   +  GF    S++ S  L ++        FV 
Sbjct: 499 ASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGFSEVVSSY-SPSLFKR--------FVS 558

Query: 546 LRVLIVDSMAVNELPDSICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRF--SPGLYE 605
           LRVL + +    +LP S+  L HLRYLD+S  KI  +P+ +  L NLQTL       L  
Sbjct: 559 LRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSLPKRLCKLQNLQTLDLYNCQSLSC 618

Query: 606 LPENLRELVSLRHLVCDR--LKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLK 665
           LP+   +L SLR+LV D   L  MP  +  L  L+TL YF VG  K  ++ EL  + NL+
Sbjct: 619 LPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNL-NLR 678

Query: 666 GSLILENLERVGSKEEAKNAKLVEKENLHELRLRWSYRSEVKHHNDLEVLEGLQPHKSLR 725
           G++ + +LERV +  EAK A L  K NLH L + W  R       +++VLE L+PH +L+
Sbjct: 679 GAISITHLERVKNDMEAKEANLSAKANLHSLSMSWD-RPNRYESEEVKVLEALKPHPNLK 738

Query: 726 HLEIDGLLGGCFP---NKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHS 749
           +LEI    G C P   N    +N+VSI +    +C  LP  G+L  LE LE+    +  S
Sbjct: 739 YLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLEL----QDGS 798

BLAST of Spg010593 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 316.2 bits (809), Expect = 1.0e-84
Identity = 243/701 (34.66%), Postives = 360/701 (51.36%), Query Frame = 0

Query: 79  GSDSEVSSLVDQVIE-ASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIWNL 138
           G D E   +V  +I  AS  Q  S+LPI+GMGG GKTTL++++F+   +   F   IW  
Sbjct: 153 GRDKEKDEIVKILINTASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWIC 212

Query: 139 EDNPSDCSDNR------EALLG-------------KLRRKMDDKRYFLVLDDVWNEDKMN 198
             +  D ++ R      E++ G             KL+  ++ KRYFLVLDDVWNED+  
Sbjct: 213 ISD--DFNEKRLIKAIVESIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHK 272

Query: 199 WEKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANAN 258
           W  L+  L      SG  +L TTR  +  ++M   +  ++L  L+ + CW LF++ A  +
Sbjct: 273 WANLRAVLK--VGASGAFVLTTTRLEKVGSIM-GTLQPYELSNLSPEDCWFLFMQRAFGH 332

Query: 259 EVPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTPLKKDDFV 318
           +  + P+L  + +E+VKK GG+PL AK LG  ++F  +  +W  +    +    + +  +
Sbjct: 333 QEEINPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSI 392

Query: 319 TFTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQPEEGNNETMEEI 378
               +LS   LP   ++QCF YC+ FPKD +  KE LI  WMA GF    +GN E +E++
Sbjct: 393 LPALRLSYHHLPL-DLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFLL-SKGNLE-LEDV 452

Query: 379 GDRYFNILLSPCLFQDIEYDGERIISCKMHDLIHDIACDISNKKILIQLDHSKPSNWELW 438
           G+  +N L     FQ+IE +  +    KMHDLIHD+A        L   + S  +  E+ 
Sbjct: 453 GNEVWNELYLRSFFQEIEVESGKTY-FKMHDLIHDLATS------LFSANTSSSNIREIN 512

Query: 439 TKEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDSIC 498
              D      +  GF    S++ S  LLQK        FV LRVL + +  +N+LP SI 
Sbjct: 513 ANYD---GYMMSIGFAEVVSSY-SPSLLQK--------FVSLRVLNLRNSNLNQLPSSIG 572

Query: 499 KLKHLRYLDIS-RTKIKKIPESIVLLYNLQT--LRFSPGLYELPENLRELVSLRHLVCD- 558
            L HLRYLD+S   +I+ +P+ +  L NLQT  L +   L  LP+   +L SLR+L+ D 
Sbjct: 573 DLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDG 632

Query: 559 -RLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKEEAK 618
             L   P  +  L  L++L  F +G  K  ++ EL  + NL GS+ +  L+RV    +AK
Sbjct: 633 CSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAK 692

Query: 619 NAKLVEKENLHELRLRWSYRSEVKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCFP---NK 678
            A L  K NLH L L W    + KH  D EVLE L+PH +L++LEI+G  G   P   N+
Sbjct: 693 EANLSAKANLHSLCLSWDL--DGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQ 752

Query: 679 IFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYSNQRTLFPK 738
              +N+VSIR+    +C  LP  G+L  LE LE+H         +  Y   +     FP 
Sbjct: 753 SVLKNVVSIRIRGCENCSCLPPFGELPCLESLELH-----TGSADVEYVEDNVHPGRFPS 812

Query: 739 LKYL---DLSKMIDLVQWEEVTDASKVATFPRLQELHISFC 749
           L+ L   D S +  L++ E          FP L+E+   +C
Sbjct: 813 LRKLVIWDFSNLKGLLKMEGEKQ------FPVLEEMTFYWC 812

BLAST of Spg010593 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.9e-79
Identity = 230/697 (33.00%), Postives = 350/697 (50.22%), Query Frame = 0

Query: 79  GSDSEVSSLVDQVI-EASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIWNL 138
           G D E   +V  +I   +  +   + PI+GMGG GKTTLA+++F+   +  HF+  IW  
Sbjct: 155 GRDKEEDEIVKILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVC 214

Query: 139 EDNPSDCSDNREALLG-----------------KLRRKMDDKRYFLVLDDVWNEDKMNWE 198
             +  D     + ++G                 KL+  ++ KRY LVLDDVWN+D   W 
Sbjct: 215 VSDDFDEKRLIKTIIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWA 274

Query: 199 KLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANEV 258
           KL+  LT   +  G SIL TTR  +  ++M   +  + L  L+      LF++ A   + 
Sbjct: 275 KLRAVLTVGAR--GASILATTRLEKVGSIM-GTLQPYHLSNLSPHDSLLLFMQRAFGQQK 334

Query: 259 PMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTPLKKDDFVTF 318
              P+L  + +E+VKK GG+PL AK LG  ++F  +  +W  +    + +  + +  +  
Sbjct: 335 EANPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILP 394

Query: 319 TFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQPEEGNNETMEEIGD 378
             +LS   LP   ++QCFAYC+ FPKD +  KE LI +WMA GF    +GN E +E++G+
Sbjct: 395 ALRLSYHHLPL-DLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLL-SKGNLE-LEDVGN 454

Query: 379 RYFNILLSPCLFQDIEYDGERIISCKMHDLIHDIACDISNKKILIQLDHSKPSNWELWTK 438
             +N L     FQ+IE         K+HDLIHD+A  + +         +  S   +   
Sbjct: 455 EVWNELYLRSFFQEIEAKSGNTY-FKIHDLIHDLATSLFS---------ASASCGNIREI 514

Query: 439 EDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDSICKL 498
             K Y   +  GF    S++ S  LL+K        FV LRVL +    + +LP SI  L
Sbjct: 515 NVKDYKHTVSIGFAAVVSSY-SPSLLKK--------FVSLRVLNLSYSKLEQLPSSIGDL 574

Query: 499 KHLRYLDISRTKIKKIPESIVLLYNLQTLRFSP--GLYELPENLRELVSLRHLVCD--RL 558
            HLRYLD+S    + +PE +  L NLQTL       L  LP+   +L SLRHLV D   L
Sbjct: 575 LHLRYLDLSCNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPL 634

Query: 559 KQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKEEAKNAK 618
              P  +  L  L+TL +F VG +K  ++ EL  + NL GS+ + +LERV +  +A+ A 
Sbjct: 635 TSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNL-NLCGSISITHLERVKNDTDAE-AN 694

Query: 619 LVEKENLHELRLRWSYRSEVKHHN-DLEVLEGLQPHKSLRHLEIDGLLGGCFP---NKIF 678
           L  K NL  L + W      ++ + +++VLE L+PH +L++LEI    G  FP   N   
Sbjct: 695 LSAKANLQSLSMSWDNDGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSV 754

Query: 679 SENLVSIRLGYGRDCEKLPMLGQLANLERLEI-HYFPRVHSIGNEFYGNYSNQRTLFPKL 738
            E ++S+R+   ++C  LP  G+L  LE LE+ +    V  +  +   +  + R  FP L
Sbjct: 755 LEKVISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSL 814

Query: 739 KYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFC 749
           K L +     L    +     K   FP L+E+ I +C
Sbjct: 815 KKLRIWFFRSLKGLMKEEGEEK---FPMLEEMAILYC 821

BLAST of Spg010593 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 2.0e-75
Identity = 220/715 (30.77%), Postives = 366/715 (51.19%), Query Frame = 0

Query: 101 SILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIW------------------NLEDNPS 160
           +++ IVG+GG GKTTL++LL++   ++++F T +W                  ++   P 
Sbjct: 197 TVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPC 256

Query: 161 DCSDNREALLGKLRRKMDDK--RYFLVLDDVWNEDKMNWEKLKECLTTITKKSGNSILIT 220
           + +D  + L  KL+ ++      + LVLDD+WNE+  +W+ L++    I    G+ IL+T
Sbjct: 257 EFTD-LDVLQVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQ--PFIHAAQGSQILVT 316

Query: 221 TRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANEVP-MTPDLELVREELVKKIGG 280
           TRS   A++M   V  H L  L+   CW+LF+K+   N+ P +  ++  + E +V K  G
Sbjct: 317 TRSQRVASIM-CAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRG 376

Query: 281 IPLVAKVLGRAVQFPGDHDQWVTMLRC-VVTTPLKKDDFVTFTFKLSVDRLPKPSIKQCF 340
           +PL  K LG  ++F G   +W  +L   +   P  K + +    ++S   LP   +K+CF
Sbjct: 377 LPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLP-VLRVSYYYLP-AHLKRCF 436

Query: 341 AYCSNFPKDFEFDKEELIQMWMAQGFTQPEEGNNETMEEIGDRYFNILLSPCLFQDIEYD 400
           AYCS FPK   F+K++++ +WMA+GF Q +  +++ +EE+G+ YF+ L S  L Q  +  
Sbjct: 437 AYCSIFPKGHAFEKDKVVLLWMAEGFLQ-QTRSSKNLEELGNEYFSELESRSLLQKTK-- 496

Query: 401 GERIISCKMHDLIHDIA--------------CDISNKK-----ILIQLDHSKPSNWELWT 460
             R I   MHD I+++A              C +   +       ++ ++++P  +E   
Sbjct: 497 -TRYI---MHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEA-L 556

Query: 461 KEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIV-EFVGLRVLIVDSMAVNELPDSIC 520
           +E K   + LP        +      L +++ +K++     LRVL +    +  LP    
Sbjct: 557 REVKFLRTFLPLSLTNSSRSC----CLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFF 616

Query: 521 K-LKHLRYLDISRTKIKKIPESIVLLYNLQTLRFS--PGLYELPENLRELVSLRH--LVC 580
           K + H R+LD+SRT+++K+P+S+  +YNLQTL  S    L ELP ++  L++LR+  L+ 
Sbjct: 617 KNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG 676

Query: 581 DRLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKEEAK 640
            +L+QMP    +L  LQTL  F V      +I+ELG + +L G L +  L+RV    +A 
Sbjct: 677 TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAA 736

Query: 641 NAKLVEKENLHELRLRW-----SYRSEVKHH---NDLEVLEGLQPHKSLRHLEIDGLLGG 700
            A L  K++L E+   W     S  +    H   N+ EV E L+PH+ +  L I+   G 
Sbjct: 737 EANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGR 796

Query: 701 CFPNKIFS---ENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYS 752
            FP+ +       +V IRL   + C  LP LGQL  L+ L I     + SIG +FY  +S
Sbjct: 797 RFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFY--FS 856

BLAST of Spg010593 vs. ExPASy TrEMBL
Match: A0A0A0LNG8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G433340 PE=4 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 1.2e-229
Identity = 461/889 (51.86%), Postives = 583/889 (65.58%), Query Frame = 0

Query: 1   MKLAAGQIDRVWNLEKDLKRLRNQLVVSEELLSRINTGE----CKESL-KQWVEDLHEIV 60
           + L A QI     ++  L++L+ +LV S++++S I T        +SL  QWV DL  IV
Sbjct: 18  LTLVAEQIILAREVKDVLQQLQKELVESQKIVSAITTQRQNHYSPDSLVTQWVNDLQLIV 77

Query: 61  LEVEDLLDELVYEHLRQKV----------------------------------------- 120
            E +DLLD  VYEHL+Q+V                                         
Sbjct: 78  HEADDLLDLFVYEHLQQRVNPSAHGKIIKKVPHFLCASARTKKMKEIIALLNKHCTKLPH 137

Query: 121 ---------------------------ELWEGSDSEVSSLVDQVIEASQRQVNSILPIVG 180
                                      +   G + EV ++VD+VI+AS++++NSILP+ G
Sbjct: 138 LLQLEPTPSNIAETEVAQIQETVSKPEDYVVGRNREVETIVDRVIDASKQELNSILPVFG 197

Query: 181 MGGSGKTTLAKLLFHHHSIKAHFDTTIW------------------NLEDNPSDCSDNRE 240
           MGG GKTTLAK +F+H  IK HF  TIW                   +E + SDCS+NRE
Sbjct: 198 MGGLGKTTLAKSVFNHDRIKNHFGITIWIYVSQPFVINNILQAILQKVEVHSSDCSNNRE 257

Query: 241 ALLGKLRRKMDDKRYFLVLDDVWNEDKMNWEKLKECLTTITKKSGNSILITTRSTEGANV 300
           ALL KL   M +K YFLVLDDVWNE+KM WEKLKECL +IT  SGNSIL+TTRS+  A +
Sbjct: 258 ALLEKLTENMGEKTYFLVLDDVWNENKMLWEKLKECLMSITHMSGNSILVTTRSSGIAKM 317

Query: 301 MKQEVDAHQLCRLTKDQCWALFVKSANANEVPMTPDLELVREELVKKIGGIPLVAKVLGR 360
           M++ + +H+L +L+ DQCW++F   ANA +VPMT +LE V++E  K+IGG+PL+AKVLG 
Sbjct: 318 MEENIGSHELRKLSDDQCWSIFRNFANAKDVPMTSNLEFVQKEFDKRIGGLPLIAKVLGA 377

Query: 361 AVQFPGDHDQWVTMLRCVVTTPLKKDDFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFE 420
           AV F GDHDQWV  ++ V+TTP+K+++FV FT KLSVDRLP  S+KQCFAYCSNF K  E
Sbjct: 378 AVPFSGDHDQWVANIKSVLTTPIKEEEFVKFTLKLSVDRLPNASVKQCFAYCSNFSKGCE 437

Query: 421 FDKEELIQMWMAQGFTQPEEGNNETMEEIGDRYFNILLSPCLFQDIEYDGERII-SCKMH 480
           FDK+++I+MWMAQGFTQP+E NNETME+ G+RYFNILLS CLFQD+  +   II   +MH
Sbjct: 438 FDKKQVIRMWMAQGFTQPDERNNETMEDTGERYFNILLSFCLFQDVVKNERGIIEKVRMH 497

Query: 481 DLIHDIACDISNKKILIQLDHSKPSNWELWTKEDKKYASKLPYGFFYDDSTWVSHGLLQK 540
           DLIHDIAC +SN K L ++DH   SNW+ WTK+DK   SKL    FYD            
Sbjct: 498 DLIHDIACQVSNDKKL-RIDHIISSNWKDWTKDDKILVSKLRTINFYDRH--------HV 557

Query: 541 VIHDKIVEFVGLRVLIVDSMAVNELPDSICKLKHLRYLDISRT-KIKKIPESIVLLYNLQ 600
           V+ DKI +F GLRVL +++  V ELP+SI KLKHLRYLDIS    IKK+PESIVLLYNLQ
Sbjct: 558 VVQDKIGDFTGLRVLTIENYIVEELPNSIFKLKHLRYLDISYCYSIKKLPESIVLLYNLQ 617

Query: 601 TLRF---SPGLYELPENLRELVSLRHLVCDRL-KQMPAYLSQLIQLQTLPYFRVGFEKCR 660
           TLRF   S G   LP+N+ +++SLRHL    + KQM  YLSQLIQL+TLP F VGFEK  
Sbjct: 618 TLRFHLLSKGF--LPKNVGQMISLRHLEFSSIDKQMSPYLSQLIQLETLPKFAVGFEKGC 677

Query: 661 KITELGPMKNLKGSLILENLERVGSKEEAKNAKLVEKENLHELRLRWS--YRSEVKHHND 720
           KITELG ++NLKG L L+ LE V SKEEA+ AKLVEKENL E+   W+   + +V++ ND
Sbjct: 678 KITELGVLRNLKGLLKLQRLEHVESKEEAETAKLVEKENLEEVHFVWTKERKRKVENKND 737

Query: 721 LEVLEGLQPHKSLRHLEIDGLLGGCFPNKIFSENLVSIRLGYGRDCEKLPMLGQLANLER 780
           LEVLEGLQP K++ +L I   LGGC PN+ F ENLV I L    +CEKLP LGQL NLE 
Sbjct: 738 LEVLEGLQPPKNVEYLRIKYFLGGCLPNQTFVENLVKIELRDCGNCEKLPRLGQLGNLEI 797

Query: 781 LEIHYFPRVHSIGNEFYGNYS-NQRTLFPKLKYLDLSKMIDLVQWEEVTDASKVATFPRL 790
           L+I +F RV SIGNEFYGN S NQR+LFP+LK L + +M  + +WEEV   S V  FPRL
Sbjct: 798 LDISWFERVKSIGNEFYGNSSNNQRSLFPRLKELYVDEMRRIGEWEEV--GSNVKAFPRL 857

BLAST of Spg010593 vs. ExPASy TrEMBL
Match: A0A6J1FWC6 (disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 7.6e-224
Identity = 447/874 (51.14%), Postives = 568/874 (64.99%), Query Frame = 0

Query: 1   MKLAAGQIDRVWNLEKDLKRLRNQLVVSEELLSRINTGEC-KESLKQWVEDLHEIVLEVE 60
           ++LAA +I   W LEK+L  L+  L+ SE  L  INT +   +S++ WVE LH +V E +
Sbjct: 18  VRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHNDSVRLWVEKLHHLVHEAD 77

Query: 61  DLLDELVYEHLRQKVELWE----------------------------------------- 120
           DL+D LVYEHLR KV+  +                                         
Sbjct: 78  DLIDNLVYEHLRHKVQKNDKMNKLNKHDREATRLGLVGDEPIQTLLQSDVSQIRETVSEV 137

Query: 121 ------GSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDT 180
                 G D EVSS+V QVI+A  ++V SILPIVGMGG GKTTLA+L+F+H  IK HFD 
Sbjct: 138 DDFEVVGRDVEVSSIVKQVIDAHNQRVTSILPIVGMGGLGKTTLARLIFNHEMIKQHFDE 197

Query: 181 TIWNLEDNPSD-----------------CSDNREALLGKLRRKMDDKRYFLVLDDVWNED 240
           TIW     P D                 C  NRE LL +L+++M  KRYFLVLDDVWNED
Sbjct: 198 TIWVCVSIPFDINKILGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNED 257

Query: 241 KMNWEKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSA 300
            + WE+LK  L  IT KSGNSIL+TTR+ E   +M++ +  H + +L+ +QCW+LF++SA
Sbjct: 258 SLLWEQLKNFLMKITSKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESA 317

Query: 301 NANEVPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTP--LK 360
           NAN +P T +LE VREELVKK GGIPLVA+VLG AV+F  D+D WV++L  V+ +P  L+
Sbjct: 318 NANGLPTTSNLEAVREELVKKFGGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQ 377

Query: 361 KDDFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQPEEGNN- 420
             +F     KLSVD LP  S+K CFAYCSNFPKD+EF K+ LIQMW+AQGF Q +EG + 
Sbjct: 378 GKNFCLPILKLSVDYLPS-SLKPCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSC 437

Query: 421 -ETMEEIGDRYFNILLSPCLFQDIEYDGE-RIISCKMHDLIHDIACDISNKKILIQLDHS 480
            +T+EE G+RYF ILLS CLFQD+  D   RIISCKMHDLIHD+ACD+SN +  + LD  
Sbjct: 438 YQTVEEEGERYFKILLSRCLFQDVVKDARGRIISCKMHDLIHDMACDVSNDQKSL-LDSC 497

Query: 481 KPSNWELWTKEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAV 540
             S+ ELW    K   SKL     ++    + H  ++K +HDKI  FV LRVLI++S ++
Sbjct: 498 TSSDEELWRNRTKNIGSKLRMIACHEG---MPH-RIEKAVHDKIGSFVCLRVLIMNSRSI 557

Query: 541 NELPDSICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRF-SPGLYELPENLRELVSLR 600
            +LP SI +LKHLRY+DIS   I+K+P+SIVLLYNL+TLR  S  + ELP++LR LV+LR
Sbjct: 558 YKLPYSIGELKHLRYVDISGCLIQKLPKSIVLLYNLKTLRIGSSPIEELPKDLRRLVNLR 617

Query: 601 HLVCDRL-----KQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLE 660
           HL    +     K+MP +LSQLI LQTL  F VGFEK  +I ELGP+KNLKGSL + NLE
Sbjct: 618 HLEFSEIYFVDPKKMPPHLSQLIHLQTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLE 677

Query: 661 RVGSKEEAKNAKLVEKENLHELRLRWSYRSEVK----HHNDLEVLEGLQPHKSLRHLEID 720
           RV S+EEAK+A  +EKE L EL L W + +E +    +HN  +VLEGLQPHK L  L I 
Sbjct: 678 RVKSREEAKSANFMEKEKLRELVLEWGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIH 737

Query: 721 GLLGGCFPNKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGN 780
             +G C  N IF +NLV+I L   R+C +LPMLG L +LE L +     V SIG+EFYGN
Sbjct: 738 SFVGECLANNIFVDNLVAISLVDCRNCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYGN 797

Query: 781 YSNQRTLFPKLKYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFCRRLLKIPDLFGY 795
           +SNQR LFPK+K   L ++  L QWEEVT  + V TFP+LQ L IS C RLL I DLFG 
Sbjct: 798 HSNQRALFPKVKIFKLIEVERLEQWEEVTVGAHVTTFPQLQVLEISKCPRLLSISDLFGC 857

BLAST of Spg010593 vs. ExPASy TrEMBL
Match: A0A6J1D340 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017143 PE=4 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 5.1e-212
Identity = 412/747 (55.15%), Postives = 521/747 (69.75%), Query Frame = 0

Query: 79  GSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHF-DTTIW-- 138
           G D E+SS+VD+VI+A+ R V SILPIVG+GG GKTTLAKL+ +H  IK HF + TIW  
Sbjct: 169 GRDVELSSIVDRVIDATNRHVTSILPIVGVGGLGKTTLAKLVINHERIKHHFNNNTIWVC 228

Query: 139 ----------------NLEDNPSDCSDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNW 198
                            L+D PS     +E LLG+L ++M  K+YFLVLDDVWNE+++ W
Sbjct: 229 VSQPFNVNKILGTILETLKDTPSGLEYKKEVLLGQLYKEMRGKKYFLVLDDVWNENRLLW 288

Query: 199 EKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANE 258
           ++LK CL  IT+ S N IL+TTRS E A +M + + +H L +L+ DQCW+LF +SANAN 
Sbjct: 289 DELKNCLMKITENSENGILVTTRSAEVAKIM-ETLPSHHLGKLSDDQCWSLFQESANANG 348

Query: 259 VPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTPLKKDDFVT 318
           + MT  L++VREELVKKIGG+PL+AKV G A Q+ GD D+WV  L+ +V   L+ ++  T
Sbjct: 349 LSMTSSLKVVREELVKKIGGLPLIAKVWGSAEQYLGDPDKWVINLKSLVCNSLQNENSAT 408

Query: 319 FTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQ-PEEGNNETMEEI 378
              KLSVDRLP+ S+KQCFAYCSNFPKDFEF KE+LIQMW+AQGF   P+  +NET+E+ 
Sbjct: 409 SALKLSVDRLPRHSLKQCFAYCSNFPKDFEFTKEQLIQMWIAQGFIHLPKGRSNETIEDE 468

Query: 379 GDRYFNILLSPCLFQDIEYDGERII-SCKMHDLIHDIACDISNKKILIQLDHSKPS-NWE 438
           GDR+F ILL  CLFQD+  D   II SCKMHDLIHDIACD+SN     +L +S  S + E
Sbjct: 469 GDRHFKILLRRCLFQDVVQDDRGIIVSCKMHDLIHDIACDVSNDH---KLQYSNNSLDGE 528

Query: 439 LWTKEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDS 498
           +WT E+KK ASKL    + +D   +   +++KVIHDK++ FV LRVL ++S +++ELPDS
Sbjct: 529 VWTDENKKIASKLRTIIYCEDQ--MMPPIMEKVIHDKVMNFVCLRVLKMNSRSISELPDS 588

Query: 499 ICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRFSPGLY-ELPENLRELVSLRHLV--- 558
           I +LKHLRYLDIS   I ++P+SI LLYNLQTL     L  +LP+N+ +LV+L+HL    
Sbjct: 589 IGELKHLRYLDISGCSITQLPKSIALLYNLQTLNMGGLLIKDLPKNIIDLVNLKHLQFYP 648

Query: 559 --CDRLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKE 618
              D  K MPAY  +L QLQTL YF VGFE   KI+ELG +KNLKGSL L  LE+V SKE
Sbjct: 649 YHFDTYK-MPAYFGKLTQLQTLSYFVVGFENGHKISELGLLKNLKGSLNLCFLEKVESKE 708

Query: 619 EAKNAKLVEKENLHELRLRWS---YRSEVKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCF 678
           EA+ AKLVEKENL EL   WS    R +   + D E+LEGLQPHK+LR L I+       
Sbjct: 709 EARAAKLVEKENLDELEFEWSQSHLRKDNDSYKDFEILEGLQPHKNLRSLSIERSAIKSL 768

Query: 679 PNKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYSNQRTL 738
           PN IF ENL  I L   ++CE+LPMLGQL NLERLE++    V SI NEFYGN  NQRTL
Sbjct: 769 PNNIFVENLAVISLRDCQNCERLPMLGQLRNLERLEVYGLHSVQSISNEFYGNDCNQRTL 828

Query: 739 FPKLKYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFCRRLLKIPDLFGYCDENGEK 795
           FPKL+   LS M +L QW+EVT AS + TFP L+ L+I  C +L+ IPD+FGYCDENG +
Sbjct: 829 FPKLRAFLLSDMENLEQWKEVTVASNLTTFPCLESLNIWRCPKLMNIPDVFGYCDENGVE 888

BLAST of Spg010593 vs. ExPASy TrEMBL
Match: A0A6J1DXY1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111025175 PE=4 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 9.7e-203
Identity = 405/720 (56.25%), Postives = 498/720 (69.17%), Query Frame = 0

Query: 79  GSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIWNLE 138
           G D EVS +V+Q+I+A+   V SILPIVGMGG GKTT+AK++F+H  IK HFD TIW   
Sbjct: 169 GRDVEVSKIVNQLIDATNTHVTSILPIVGMGGLGKTTMAKVVFNHERIKTHFDKTIWVCV 228

Query: 139 DNP-----------------SDCSDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNWEK 198
             P                 S   DN+EALL +L+++M  KRYFLVLDDVWNE    W++
Sbjct: 229 SEPFLVNKILGAILGTLRGTSSGLDNKEALLHELKKEMHGKRYFLVLDDVWNESHSLWDE 288

Query: 199 LKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANEVP 258
           LK CL  IT++S NSIL+TTRS E A +M + +  H L +L+ DQCW+LF++SAN N + 
Sbjct: 289 LKNCLMRITERSENSILVTTRSAEVAKIM-ETLPNHHLSKLSDDQCWSLFLESANVNGLS 348

Query: 259 MTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTT--PLKKDDFVT 318
           +T  L++V +ELVKKIGGIPLVA+VLGRAV+F GD++ WVT L  ++ T   L+++ FV 
Sbjct: 349 ITSSLKVV-QELVKKIGGIPLVARVLGRAVKFEGDYETWVTTLESIIRTRLQLQEESFVL 408

Query: 319 FTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQ-PEEGNNETMEEI 378
              KLSVDRLP  S+KQCF+YCSNFPKDF F+KE LI+MWMAQGF Q  E  +N+TME+ 
Sbjct: 409 SILKLSVDRLPLSSLKQCFSYCSNFPKDFVFEKEPLIRMWMAQGFIQLQERRSNKTMEDE 468

Query: 379 GDRYFNILLSPCLFQDIEYDGE-RIISCKMHDLIHDIACDISNKKILIQLDHSKPSNWEL 438
           GD YF ILLS CLFQDI  DG+ RIIS KMHDLIHDIAC+ISN K L  LD     + E 
Sbjct: 469 GDTYFKILLSRCLFQDIIKDGKGRIISFKMHDLIHDIACNISNDKKL-HLDPCNLLDNEH 528

Query: 439 WTKE-DKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDS 498
           W  E  +K ASKL       ++        ++VI DK+  F+ LRVLI++     +LPDS
Sbjct: 529 WRNEISEKVASKLRTVICRRETL----HRREQVIDDKMSNFIWLRVLIINFWNAKKLPDS 588

Query: 499 ICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRF-SPGLYELPENLRELVSLRHLV--- 558
           I KLKHLRYLDIS   + ++PESIV LY+LQTLR     + +LP+NL +LVSLRHL    
Sbjct: 589 IGKLKHLRYLDISGCSMDELPESIVSLYHLQTLRIEGTMIVDLPKNLGKLVSLRHLEFSF 648

Query: 559 ---CDRLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSK 618
                ++ QMP++LSQL+QLQTL  F VGFEK RKITELGP+KNLKGSL L NLER+ SK
Sbjct: 649 FLGIKKILQMPSHLSQLVQLQTLSTFIVGFEKGRKITELGPLKNLKGSLNLSNLERIKSK 708

Query: 619 EEAKNAKLVEKENLHELRLRWSYRS--EVKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCF 678
           EEA  AKL EKENLHEL   W+  S  E  +HNDL+VLEGLQPHKSL+ L I   LG C 
Sbjct: 709 EEAMFAKLGEKENLHELTFTWTCSSEREYNNHNDLDVLEGLQPHKSLQSLSIQSFLGECL 768

Query: 679 PNKIFSENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYSNQRTL 738
           PNKIF ENLV+I L    +CE+LPMLGQL NLE L I     V SIGNEFYGN S+QRT 
Sbjct: 769 PNKIFVENLVAISLVDCENCERLPMLGQLTNLEELCISGLHSVRSIGNEFYGNDSDQRTF 828

Query: 739 FPKLKYLDLSKMIDLVQWEEVTDASKVATFPRLQELHISFCRRLLKIPDLFGYCDENGEK 768
           FPKLK   L+KM +L QWEEVT A  +  FP ++   IS C +LLK+P +FG  D+N  K
Sbjct: 829 FPKLKKFTLNKMSNLEQWEEVTLAPNLTVFPCIEMWKISECFKLLKVPKIFGSWDKNHVK 881

BLAST of Spg010593 vs. ExPASy TrEMBL
Match: A0A5D3D021 (Putative disease resistance protein RGA3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001110 PE=4 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 1.8e-193
Identity = 418/903 (46.29%), Postives = 533/903 (59.03%), Query Frame = 0

Query: 1   MKLAAGQIDRVWNLEKDLKRLRNQLVVSEELLSRINTGEC-KESLKQWVEDLHEIVLEVE 60
           + LAA +I   W LEK+L  L   L+ SE +L  IN  +    S++ WV DL  +V E +
Sbjct: 18  LTLAADKIGLAWGLEKELSELSQWLLKSEAILGDINRKKLHPSSVRLWVADLQLVVHEAD 77

Query: 61  DLLDELVYEHLRQKVELW------------------------------------------ 120
           DLLDELVYEHLR KVE                                            
Sbjct: 78  DLLDELVYEHLRTKVEKGLINKVCYSVSSGSNIFIIFRFKMVKKVRIIIEKLRKCYFEAA 137

Query: 121 -------------------------------EGSDSEVSSLVDQVIEASQRQVNSILPIV 180
                                           G + EVSS+V Q+++AS R V +ILPIV
Sbjct: 138 PLGLVGEEFIETENDLGQIRETISKLDDFEVVGREFEVSSIVKQMVDASDRYVTTILPIV 197

Query: 181 GMGGSGKTTLAKLLFHHHSIKAHFDTTIWNLEDNP-----------------SDCSDNRE 240
           GMGG GKTTLAK +FHH  I+ HFD TIW     P                 S   DN+E
Sbjct: 198 GMGGIGKTTLAKTIFHHEEIRGHFDETIWICVSEPFLINKILGAILQMIKGVSSGLDNKE 257

Query: 241 ALLGKLRRKMDDKRYFLVLDDVWNEDKMNWEKLKECLTTITKKSGNSILITTRSTEGANV 300
           ALL +L++ M  KRYFLVLDDVWNE+   W +LK CL + T+KS N I++TTRS E   +
Sbjct: 258 ALLRELQKVMRGKRYFLVLDDVWNENLALWTELKNCLLSFTEKSENGIIVTTRSVEVGKI 317

Query: 301 MKQEVDAHQLCRLTKDQCWALFVKSANANEVPMTPDLELVREELVKKIGGIPLVAKVLGR 360
           M+  + +H L +L+ +QCW+LF KSANANE+PM  +L+ ++EELVK+ GG+PLVA+VLG 
Sbjct: 318 MECTLSSHHLGKLSDEQCWSLFKKSANANELPMNLELKDIQEELVKRFGGVPLVARVLGG 377

Query: 361 AVQFPGDHDQWVTMLRCVVTTPLKKDDFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFE 420
           A++F G +++WV  LR   + PL+ +D V  T KLSVDRLP  S+K+CFAYCSNF K F+
Sbjct: 378 ALKFEGVYEKWVMSLRTTTSIPLQDEDLVLSTLKLSVDRLPSFSLKRCFAYCSNFSKGFK 437

Query: 421 FDKEELIQMWMAQGFTQPEEG-NNETMEEIGDRYFNILLSPCLFQD-IEYDGERIISCKM 480
           F KEELIQMWMAQGF Q  EG NN TMEE G++YFNILLS  LFQD I+ D  RI  CKM
Sbjct: 438 FRKEELIQMWMAQGFIQLHEGINNITMEENGEKYFNILLSRSLFQDIIKDDRGRITHCKM 497

Query: 481 HDLIHDIACDISNKKILIQ-----LDHSKPSNWELWTKEDKKYASKLPYGFFYDDSTWVS 540
           HDLI++IAC ISN + L Q     LD    +N  +   ++ +             +   +
Sbjct: 498 HDLIYEIACIISNSQKLQQEQIDLLDKGSRTNHRINNAQNLR-------------TLICN 557

Query: 541 HGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDSICKLKHLRYLDISRTKIKKIPESIVL 600
             +L K I+ KI     LRVL+VDS ++ +LP+SI K+KHLRYLDIS +KI+++P SI L
Sbjct: 558 RQMLHKTIYGKIANCTRLRVLVVDS-SITKLPESIGKMKHLRYLDISNSKIEELPNSISL 617

Query: 601 LYNLQTLRFSPGLYELPENLRELVSLRHLVCDRLKQMPAYLSQLIQLQTLPYFRVGFEKC 660
           L+NLQTL+F   +  LP+NL +L +LRHL    + Q P +LS+L QLQTL  F VGFEK 
Sbjct: 618 LHNLQTLKFGSSMKHLPQNLSKLANLRHLKFS-IPQTPPHLSRLTQLQTLSGFAVGFEKG 677

Query: 661 RKITELGPMKNLKGSLILENLERVGSKEEAKNAKLVEKENLHELRLRWSYR--SEVKHHN 720
            KI ELG +K+LKG L L NL+R+ +KEEA ++KLVEK NL EL L W      E   +N
Sbjct: 678 CKIGELGFLKDLKGRLELSNLDRIENKEEAMSSKLVEK-NLCELLLEWDLHILRECSSYN 737

Query: 721 DLEVLEGLQPHKSLRHLEIDGLLGGCFPNKIFSENLVSIRLGYGRDCEKLPMLGQLANLE 780
           DLEVLEGLQPHK+L+ L I    G   P  IF ENL  I L +   CE LPMLGQL NLE
Sbjct: 738 DLEVLEGLQPHKNLQFLSIINFAGQLLPAAIFVENLAVIHLRHCVRCEALPMLGQLPNLE 797

Query: 781 RLEIHYFPRVHSIGNEFYGNY----SNQRTLFPKLKYLDLSKMIDLVQWEEVTDASKV-A 799
            L I     + SIGNEFYGNY    +N + LFPKLK   LS M +L QWE +   SK  A
Sbjct: 798 ELNISNLLCLRSIGNEFYGNYDHPNNNHKVLFPKLKKFVLSHMHNLEQWEGLVFTSKKDA 857

BLAST of Spg010593 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 285.4 bits (729), Expect = 1.4e-76
Identity = 220/715 (30.77%), Postives = 366/715 (51.19%), Query Frame = 0

Query: 101 SILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIW------------------NLEDNPS 160
           +++ IVG+GG GKTTL++LL++   ++++F T +W                  ++   P 
Sbjct: 197 TVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPC 256

Query: 161 DCSDNREALLGKLRRKMDDK--RYFLVLDDVWNEDKMNWEKLKECLTTITKKSGNSILIT 220
           + +D  + L  KL+ ++      + LVLDD+WNE+  +W+ L++    I    G+ IL+T
Sbjct: 257 EFTD-LDVLQVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQ--PFIHAAQGSQILVT 316

Query: 221 TRSTEGANVMKQEVDAHQLCRLTKDQCWALFVKSANANEVP-MTPDLELVREELVKKIGG 280
           TRS   A++M   V  H L  L+   CW+LF+K+   N+ P +  ++  + E +V K  G
Sbjct: 317 TRSQRVASIM-CAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRG 376

Query: 281 IPLVAKVLGRAVQFPGDHDQWVTMLRC-VVTTPLKKDDFVTFTFKLSVDRLPKPSIKQCF 340
           +PL  K LG  ++F G   +W  +L   +   P  K + +    ++S   LP   +K+CF
Sbjct: 377 LPLAVKTLGGVLRFEGKVIEWERVLSSRIWDLPADKSNLLP-VLRVSYYYLP-AHLKRCF 436

Query: 341 AYCSNFPKDFEFDKEELIQMWMAQGFTQPEEGNNETMEEIGDRYFNILLSPCLFQDIEYD 400
           AYCS FPK   F+K++++ +WMA+GF Q +  +++ +EE+G+ YF+ L S  L Q  +  
Sbjct: 437 AYCSIFPKGHAFEKDKVVLLWMAEGFLQ-QTRSSKNLEELGNEYFSELESRSLLQKTK-- 496

Query: 401 GERIISCKMHDLIHDIA--------------CDISNKK-----ILIQLDHSKPSNWELWT 460
             R I   MHD I+++A              C +   +       ++ ++++P  +E   
Sbjct: 497 -TRYI---MHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEA-L 556

Query: 461 KEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIV-EFVGLRVLIVDSMAVNELPDSIC 520
           +E K   + LP        +      L +++ +K++     LRVL +    +  LP    
Sbjct: 557 REVKFLRTFLPLSLTNSSRSC----CLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFF 616

Query: 521 K-LKHLRYLDISRTKIKKIPESIVLLYNLQTLRFS--PGLYELPENLRELVSLRH--LVC 580
           K + H R+LD+SRT+++K+P+S+  +YNLQTL  S    L ELP ++  L++LR+  L+ 
Sbjct: 617 KNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG 676

Query: 581 DRLKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKEEAK 640
            +L+QMP    +L  LQTL  F V      +I+ELG + +L G L +  L+RV    +A 
Sbjct: 677 TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAA 736

Query: 641 NAKLVEKENLHELRLRW-----SYRSEVKHH---NDLEVLEGLQPHKSLRHLEIDGLLGG 700
            A L  K++L E+   W     S  +    H   N+ EV E L+PH+ +  L I+   G 
Sbjct: 737 EANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGR 796

Query: 701 CFPNKIFS---ENLVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYS 752
            FP+ +       +V IRL   + C  LP LGQL  L+ L I     + SIG +FY  +S
Sbjct: 797 RFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFY--FS 856

BLAST of Spg010593 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 269.6 bits (688), Expect = 7.9e-72
Identity = 211/710 (29.72%), Postives = 348/710 (49.01%), Query Frame = 0

Query: 101 SILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIW---------------NLEDNPSDC- 160
           +++ +VGM G GKTTL +++F+ + +  HF+  +W                L+D  S   
Sbjct: 194 AVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAV 253

Query: 161 -SDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNWEKLKECLTTITKKSGNSILITTRS 220
            +++  +L  +L++ +  KR+ LVLDD W+E    WE  +   T    + G+ I++TTRS
Sbjct: 254 NTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWESFQVAFT--DAEEGSKIVLTTRS 313

Query: 221 TEGANVMKQEVDAHQLCRLTKDQCWALFVKSA--NANEVPMTPDLELVREELVKKIGGIP 280
              + V K E   +Q+  +T ++CW L  + A  N +   +  +LE + + + ++  G+P
Sbjct: 314 EIVSTVAKAE-KIYQMKLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLP 373

Query: 281 LVAKVLGRAVQFPGDHDQWVTMLRCVVTTPLKKDDFVTFT------FKLSVDRLPKPSIK 340
           L A+ +   ++   + D W  + +          +F ++T       KLS D LP P +K
Sbjct: 374 LAARAIASHLRSKPNPDDWYAVSK----------NFSSYTNSILPVLKLSYDSLP-PQLK 433

Query: 341 QCFAYCSNFPKDFEFDKEELIQMWMAQGFT-QPEEGNNETMEEIGDRYFNILLSPCLFQD 400
           +CFA CS FPK   FD+EEL+ +WMA     QP   ++  +E+IG+ Y   L++   FQ 
Sbjct: 434 RCFALCSIFPKGHVFDREELVLLWMAIDLLYQPR--SSRRLEDIGNDYLGDLVAQSFFQR 493

Query: 401 IEYDGERIISCKMHDLIHDIACDISNKKILIQLDHSKPS------NWELWTKE-DKKYAS 460
           ++     + S  MHDL++D+A  +S        D + P       ++     + D   A 
Sbjct: 494 LDI---TMTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAF 553

Query: 461 KLPYGF--------FYDDSTWVSHGLLQKVIHDKIVEFVGLRVLIVDSMAVNELPDSICK 520
           +   G         F   ++  S  L +KV++  +    GLR+L +    +  LP S+  
Sbjct: 554 RSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKG 613

Query: 521 LKHLRYLDISRTKIKKIPESIVLLYNLQTLRFS--PGLYELPENLRELVSLR--HLVCDR 580
           LK LRYLD+S TKIK++PE +  L NLQTL  S    L  LP+++ EL++LR   LV   
Sbjct: 614 LKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTP 673

Query: 581 LKQMPAYLSQLIQLQTLPYFRVGFEKCRKITELGPMKNLKGSLILENLERVGSKEEAKNA 640
           L +MP  + +L  LQ L  F +G      + EL  + +L+G+L +  L+ V    EAK+A
Sbjct: 674 LVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDA 733

Query: 641 KLVEKENLHELRLRWSYRS--------EVKHHNDLEVLEGLQPHKSLRHLEIDGLLGGCF 700
            L  K  L  L L+W+ +              +  EVL  L+PH  L+   I+   GG F
Sbjct: 734 GLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAF 793

Query: 701 PNKIFSEN---LVSIRLGYGRDCEKLPMLGQLANLERLEIHYFPRVHSIGNEFYGNYSNQ 754
           P  +   +   + S+ L     C  LP +GQL +L+ L I  F  +  +G +F+   +N 
Sbjct: 794 PKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNS 853

BLAST of Spg010593 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 173.3 bits (438), Expect = 7.7e-43
Identity = 204/749 (27.24%), Postives = 328/749 (43.79%), Query Frame = 0

Query: 79  GSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTIW--- 138
           G D  V  LVD ++E    QV S   + GMGG GKTTLA+ +FHH  ++ HFD   W   
Sbjct: 165 GLDQSVEELVDHLVENDSVQVVS---VSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCV 224

Query: 139 ------------NLED-NPSD---CSDNREALLGKLRRKMDDKRYFLVLDDVWNEDKMNW 198
                        L+D  P D      +   L G+L   ++  RY LVLDDVW E+  +W
Sbjct: 225 SQQFTRKDVWQRILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEE--DW 284

Query: 199 EKLKECLTTITKKSGNSILITTRSTEGANVMKQEV-DAHQLCRLTKDQCWALF--VKSAN 258
           +++K        K G  +L+T+R+ EG  +       A +   LT +Q W LF  + S+ 
Sbjct: 285 DRIK---AVFPHKRGWKMLLTSRN-EGLGLHADPTCFAFRPRILTPEQSWKLFERIVSSR 344

Query: 259 ANEVPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTTPLKK-- 318
            ++     D E + +E+V   GG+PL  KVLG  +       +W  +   +VT  + K  
Sbjct: 345 RDKTEFKVD-EAMGKEMVTYCGGLPLAVKVLGGLLAKKHTVLEWKRVHSNIVTHIVGKSG 404

Query: 319 --DDFVTFTFK---LSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQPEE 378
             DD     ++   LS + LP   +K CF Y ++FP+D++ D + L   W+A+G   P  
Sbjct: 405 LSDDNSNSVYRVLSLSYEDLPM-QLKHCFFYLAHFPEDYKIDVKILFNYWVAEGIITPFH 464

Query: 379 GNNETMEEIGDRYFNILL-SPCLFQDIEYDGERIISCKMHDLIHDIACDISNKKILIQLD 438
            +  T+++ G+ Y   L+    +  +  Y   RI  C+MHD++ ++    + ++  I++ 
Sbjct: 465 -DGSTIQDTGESYLEELVRRNMVVVEESYLTSRIEYCQMHDMMREVCLSKAKEENFIRVV 524

Query: 439 -----------------------HSKPSNWELWTKEDKKYASKLPYGFFYDDSTWVSHGL 498
                                  HS  +   L  K++KK  S L +G   ++  W   G 
Sbjct: 525 KVPTTTSTTINAQSPCRSRRLVLHSGNALHMLGHKDNKKARSVLIFG--VEEKFWKPRGF 584

Query: 499 ----LQKVIHDKIVEFVGLRVLIVDSMAVNELPDSICKLKHLRYLDISRTKIKKIPESIV 558
               L +V+    V+F G            +LP SI  L HLR+L +    +  +P S+ 
Sbjct: 585 QCLPLLRVLDLSYVQFEG-----------GKLPSSIGDLIHLRFLSLYEAGVSHLPSSLG 644

Query: 559 LLYNLQTLRFSPG---LYELPENLRELVSLRHLVCDRLKQMPA----YLSQLIQLQTLPY 618
            L  L  L        L  +P  L+E+  LR+L   R   MPA     L  L+ L++L  
Sbjct: 645 NLKLLLCLNLGVADRLLVHVPNVLKEMQELRYLRLPR--SMPAKTKLELGDLVNLESLTN 704

Query: 619 FRVGFEKCRKITELGPMKNLKGSLILENLERVGSKEEAKNAKLVEKENLHELRLRWSYRS 678
           F     K   +T+L  M  L    +L  +       E     L E  NL  L      + 
Sbjct: 705 FST---KHGSVTDLLRMTKLS---VLNVIFSGECTFETLLLSLRELRNLETLSFHDFQKV 764

Query: 679 EVKHH-NDLEVLEGLQPHKSLRHLEIDGLLGGCFPNKI-FSENLVSIRL-GYGRDCEKLP 738
            V +H  +L VL+ +     L+ L +   L   FP++  F  +L  I L G   + + +P
Sbjct: 765 SVANHGGELLVLDFIH----LKDLTLSMHLPR-FPDQYRFPPHLAHIWLIGCRMEEDPMP 824

Query: 739 MLGQLANLERLEIHYFPRVHSIGNEFYGNYSNQRTLFPKLKYLDLSKMIDLVQWEEVTDA 761
           +L +L +L+ +   Y      +G     +       FP+L  L +S   +LV+W      
Sbjct: 825 ILEKLLHLKSV---YLSSGAFLGRRMVCSKGG----FPQLLALKMSYKKELVEWR----- 863

BLAST of Spg010593 vs. TAIR 10
Match: AT3G46730.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 173.3 bits (438), Expect = 7.7e-43
Identity = 147/523 (28.11%), Postives = 244/523 (46.65%), Query Frame = 0

Query: 75  ELWEGSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHFDTTI 134
           EL  G + +V  L+ +++  +++  + I+ I GMGG GKT LA+ L++   +K  FD   
Sbjct: 160 ELVVGLEDDVKILLVKLLSDNEKDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRA 219

Query: 135 WNLEDNPSDCSD------------------------NREALLGKLRRKMDDKRYFLVLDD 194
           W          D                          E L   L   ++ K Y +V+DD
Sbjct: 220 WTYVSQEYKTRDILIRIIRSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDD 279

Query: 195 VWNEDKMNWEKLKECLTTITKKSGNSILITTRSTEGANVMKQEVDAHQLCRLTKDQCWAL 254
           VW+ D   WE LK  L       G+ ++ITTR    A  ++  V AH+L  LT ++ W L
Sbjct: 280 VWDPDA--WESLKRALP--CDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTL 339

Query: 255 FVKSANANEVPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTMLRCVVTT 314
           F + A +N   +  DL+   +E+VKK GG+PL   VL   +      ++W  +   +   
Sbjct: 340 FERKAFSNIEKVDEDLQRTGKEMVKKCGGLPLAIVVLSGLLSRKRT-NEWHEVCASLWRR 399

Query: 315 PLKKDDFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWMAQGFTQPEEG 374
                  ++  F LS   + +  +K CF Y S FP+D+E   E+LI + +A+GF Q +E 
Sbjct: 400 LKDNSIHISTVFDLSFKEM-RHELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDE- 459

Query: 375 NNETMEEIGDRYFNILLSPCLFQDIEYDGERIISCKMHDLIHDIACDISNKKILIQLDHS 434
               ME++   Y + L+   L +    +  +++SC++HDL+ D+A   + +   + + + 
Sbjct: 460 -EMMMEDVARCYIDELVDRSLVKAERIERGKVMSCRIHDLLRDLAIKKAKELNFVNVYNE 519

Query: 435 KPSNWELWTKE----------------DKKYASKL----PYGFFYDDSTWVSHGLLQKVI 494
           K  + ++  +E                +K+  S L      GF Y ++T +   LL    
Sbjct: 520 KQHSSDICRREVVHHLMNDYYLCDRRVNKRMRSFLFIGERRGFGYVNTTNLKLKLL---- 579

Query: 495 HDKIVEFVGLRVLIVDSMAVNELPDSICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLR 553
             +++   GL  L V     N LPD I +L HLRYL I+ T +  +P SI  L  LQTL 
Sbjct: 580 --RVLNMEGL--LFVSKNISNTLPDVIGELIHLRYLGIADTYVSILPASISNLRFLQTLD 639

BLAST of Spg010593 vs. TAIR 10
Match: AT3G07040.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 165.2 bits (417), Expect = 2.1e-40
Identity = 203/803 (25.28%), Postives = 354/803 (44.08%), Query Frame = 0

Query: 11  VWNLEKDLKRLRNQLVVSEELLSRINTGECKESLKQWVEDLHEIVLEVEDLLDELVYEHL 70
           + ++   +KR  +       LL  I+ G+ K     WV ++ E  L   +  + LV    
Sbjct: 125 IQSISDSMKRYYHSENYQAALLPPIDDGDAK-----WVNNISESSLFFSE--NSLV---- 184

Query: 71  RQKVELWEGSDSEVSSLVDQVIEASQRQVNSILPIVGMGGSGKTTLAKLLFHHHSIKAHF 130
                   G D+    L+ +++    +++  ++ +VGMGGSGKTTL+  +F   S++ HF
Sbjct: 185 --------GIDAPKGKLIGRLLSPEPQRI--VVAVVGMGGSGKTTLSANIFKSQSVRRHF 244

Query: 131 DTTIW-------NLED----------------NPSDC-SDNREALLGKLRRKMDDKRYFL 190
           ++  W        +ED                 P++  S     L+ KL   +  KRY +
Sbjct: 245 ESYAWVTISKSYVIEDVFRTMIKEFYKEADTQIPAELYSLGYRELVEKLVEYLQSKRYIV 304

Query: 191 VLDDVWNEDKMNWEKLKECLTTITKKSGNSILITTRSTEGANV-MKQEVDAHQLCRLTKD 250
           VLDDVW      W ++   L       G+ +++TTR    A+         H++  L +D
Sbjct: 305 VLDDVWTTGL--WREISIALP--DGIYGSRVMMTTRDMNVASFPYGIGSTKHEIELLKED 364

Query: 251 QCWALFVKSA--NANEVPMTPDLELVREELVKKIGGIPLVAKVLGRAVQFPGDHDQWVTM 310
           + W LF   A   + E   T +LE +  +LV++  G+PL    LG  +       +W   
Sbjct: 365 EAWVLFSNKAFPASLEQCRTQNLEPIARKLVERCQGLPLAIASLGSMMSTKKFESEW-KK 424

Query: 311 LRCVVTTPLKKD---DFVTFTFKLSVDRLPKPSIKQCFAYCSNFPKDFEFDKEELIQMWM 370
           +   +   L  +     V     LS + LP P +K+CF YCS FP ++   ++ LI+MWM
Sbjct: 425 VYSTLNWELNNNHELKIVRSIMFLSFNDLPYP-LKRCFLYCSLFPVNYRMKRKRLIRMWM 484

Query: 371 AQGFTQPEEGNNETMEEIGDRYFNILLSPCLFQDIEYDG-ERIISCKMHDLIHDIACDIS 430
           AQ F +P  G     EE+ D Y N L+   + Q I ++   R  + KMHD+I +IA  +S
Sbjct: 485 AQRFVEPIRG--VKAEEVADSYLNELVYRNMLQVILWNPFGRPKAFKMHDVIWEIALSVS 544

Query: 431 NKKILIQLDHSKPSNWELWTKEDKKYASKLPYGFFYDDSTWVSHGLLQKVIHDKIVEFVG 490
             +    + ++  S+ +   +  + Y S+              H  +QK +    +    
Sbjct: 545 KLERFCDV-YNDDSDGDDAAETMENYGSR--------------HLCIQKEMTPDSIRATN 604

Query: 491 LRVLIVDSMAVNELPDSICKLKHLRYLDISRTKIKKIPESIVLLYNLQTLRFS-PGLYEL 550
           L  L+V S A +++ + +  L  LR LD+  + I K+P+ +V ++NL+ L  S   + EL
Sbjct: 605 LHSLLVCSSAKHKM-ELLPSLNLLRALDLEDSSISKLPDCLVTMFNLKYLNLSKTQVKEL 664

Query: 551 PENLRELVSLRHLVC--DRLKQMPAYLSQLIQLQTLPYFR------------VGFEKCRK 610
           P+N  +LV+L  L     +++++P  + +L +L+ L  FR            +G     K
Sbjct: 665 PKNFHKLVNLETLNTKHSKIEELPLGMWKLKKLRYLITFRRNEGHDSNWNYVLGTRVVPK 724

Query: 611 ITELGPMK-----NLKGSLILENLERVGSKEEAKNAKLVEKENLHELRLRWSYRSEVKHH 670
           I +L  ++     N +  LI    + +G   +     LV     H   L  S  +++K  
Sbjct: 725 IWQLKDLQVMDCFNAEDELI----KNLGCMTQLTRISLVMVRREHGRDLCDSL-NKIKRI 784

Query: 671 NDLEV--LEGLQPHKSLRHLEIDGLLGGCFPNKIFSENLVSIRLGYGRDCEKLPMLG--- 730
             L +  ++  +P      LEID L+      K+F    +     +    + L  LG   
Sbjct: 785 RFLSLTSIDEEEP------LEIDDLIATASIEKLFLAGKLERVPSWFNTLQNLTYLGLRG 844

Query: 731 -QLANLERLEIHYFPRVHSIGNEFYGNYSNQRTLFPKLKYLDLSKMIDLVQWEEVTD-AS 756
            QL     L I   PR+  +   FY  Y   R  F +  + +L K++++VQ + +T+   
Sbjct: 845 SQLQENAILSIQTLPRL--VWLSFYNAYMGPRLRFAQ-GFQNL-KILEIVQMKHLTEVVI 867

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011650061.12.5e-22951.86putative disease resistance protein RGA3 [Cucumis sativus] >KGN63368.1 hypotheti... [more]
XP_022944234.11.6e-22351.14disease resistance protein RGA2-like isoform X1 [Cucurbita moschata][more]
XP_022148515.11.1e-21155.15disease resistance protein RGA2-like [Momordica charantia][more]
XP_022158717.12.0e-20256.25putative disease resistance protein RGA3 [Momordica charantia][more]
XP_038900462.14.6e-19945.76disease resistance protein RGA2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q7XBQ91.9e-9132.64Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Q7XA402.8e-9031.90Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA421.0e-8434.66Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA392.9e-7933.00Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR42.0e-7530.77Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A0A0LNG81.2e-22951.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G433340 PE=4 SV=1[more]
A0A6J1FWC67.6e-22451.14disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1D3405.1e-21255.15disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1DXY19.7e-20356.25putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5D3D0211.8e-19346.29Putative disease resistance protein RGA3 isoform X1 OS=Cucumis melo var. makuwa ... [more]
Match NameE-valueIdentityDescription
AT3G14470.11.4e-7630.77NB-ARC domain-containing disease resistance protein [more]
AT3G14460.17.9e-7229.72LRR and NB-ARC domains-containing disease resistance protein [more]
AT1G53350.17.7e-4327.24Disease resistance protein (CC-NBS-LRR class) family [more]
AT3G46730.17.7e-4328.11NB-ARC domain-containing disease resistance protein [more]
AT3G07040.12.1e-4025.28NB-ARC domain-containing disease resistance protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 44..64
NoneNo IPR availableCOILSCoilCoilcoord: 4..31
NoneNo IPR availableCOILSCoilCoilcoord: 580..609
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 257..271
score: 36.0
coord: 102..117
score: 56.72
coord: 706..722
score: 34.85
NoneNo IPR availablePANTHERPTHR23155:SF1139NBS-LRR TYPE DISEASE RESISTANCE PROTEINcoord: 81..755
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 386..795
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 89..135
e-value: 9.5E-8
score: 31.5
coord: 145..272
e-value: 3.4E-24
score: 85.4
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 3..79
e-value: 6.6E-9
score: 35.9
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 305..397
e-value: 5.5E-13
score: 50.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 59..136
e-value: 8.4E-13
score: 50.2
coord: 137..218
e-value: 2.2E-11
score: 45.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 64..321
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 221..304
e-value: 2.8E-5
score: 25.6
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 441..798
e-value: 3.9E-41
score: 142.6
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 81..755
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 482..503
score: 7.21141

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg010593.1Spg010593.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding
molecular_function GO:0005515 protein binding