Spg010295 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg010295
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProlyl endopeptidase
Locationscaffold7: 3728863 .. 3734670 (+)
RNA-Seq ExpressionSpg010295
SyntenySpg010295
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTTTTTATGTCGTTCACTTGAGAAAAAGATGACGAAACAGAAACCATTTAACTTTCAGTAAGATCAGAAACCACTATACATACGAGTGTCACCATAATTCCGGCGCCTCCATTTTGAAGCACTATGGCTCTTAAATCCCTGCTAAAACCCAAATGCTCCATTAGAAAATTCTTCCTTTCTTCTTCCTCCATATCGGTTTCGCGATTCTCGTCTCTCTGCAAAGAACGCATCTTCTCACTGCCTTCCGAATCCCCACCAGCTGCTAAAAAAGTCCCTTTCACTCACTCAGTTCATGGCGTTACGCTGCAAGATCCCTACCATTGGATGTCCAACACCGACGACCCTGATCTCGCCGACTACCTCCGTCGAGAAAACTTGTACGCTGAATCTTTCATGGCCGATACTCAGATTCTGCAGCGGCGGCTCTTCTCCGAGATGACGAGTCGAATGCCCGCCAAGGTTTCCACTCCTCCTGAGCCTTGGGGACCCTGGTTTGCTCATTTCTTTGTTGTGTGATTGCGTCTGTGGCCTTAATCTAGAGTTTTTAATTTACTTGATGATCGGTCATGGTTGAAGCTGAATGTGTCGATGAATTTGATTAGGTTTTACTACCAGTACATTCCGGAGGGGAAGGATTACCCAGTTCTATGTCGTAGGTTACAGATTGAGAAAGGCGGTTGGTTAAAGAAATTTGTACAATTTGCCAAAGGAAATTTTGGGAGGGAGGAGGAAGTTTTGATTGATTGGAATGAAATTGCTAAACAATATGGTAAGTTCATCAAGGTCTGCATTAGAAACAAGAGCGTCAATTTTTTTTTTCAGTTGCACGCTGGGAAGAGATTCTCTTTCTACTGGTCTTTTCAATGAGATCATGTCTATTTTATTTGTCTATGTCGAACTGAAGAGTTCTGAAAGTCAATCTTCATCATTTTTTTAATACTTAAAAGAACACAGATGTTTCATTTAAATTTTGAAAGTAAGGGTAACATGAGTGTAGCTCAATTGATTAAGGTAATTGTATCATCGACAAAAAGGCCAAAGGTAGGTTTGTCATCGTGTTCATCAAGGCAATCTTCATTTCTGATCTTACTTCCAGTTATGAAAATTATGGCATTCATTAATTTCTACAATGAGTTGCTCATGATTGTAGGCTATGTTCACGTGGGAACTTGCCGTGTTTCACCAGACCACAACTTTCTGGCATACACAGTTGATATTACTGGCAGTGAACACTTCATGCTTCAGGTTAAAGACCTCAGAACTGGACTGATAATTTCCAAGTTACAGGAGGGAGTTGTAAGTTTGGCTTGGGCAGAAGAAGGCAGGATGCTTTTTTATACACAAGCAGATGAGAATCAGCGACCGTACAGGCAAATTTCTGTCATCATTCTTAGCCTTCTTTGAAGAATTACATTTTCCTGACATTCCTTATTTTCTGCTGCTGCCCTAACATTTGATAGGGTTTTCTGCACAAAAGTTGGATGTACCGATATGGAAGATGTCTTGGTGTTTGTCGAAAATGATCCCAACTATTGTGTAGACATAACAAGCACAAAAGATGGGAAGTTCATAACTGTGAATTCGAACTCGAGGACTTCTTCTGAGGAAGGAACTTACCTATTTCAATTTTTTTCTGTCTGTTTGCTTTGAAGAAGATTTTATTTAAGCTAACTTTGGACTTCTAGTCTGAAACTCTGCTGGTTTGTCTAACTCAGGTTTATATTATAGATGCTAACCACTCATTAAGTGGTTTGCAAAGAATACACAAACGCATTCCTGGTATTCAGTACTTTCTGGAACATCATAGTGGTTTCTTTTATATCCTAACAAATGCTCCGCTAGAAAAGAATGGAGATTGTTCCAAGGAAGAGTATTATGTAGCTCGATGTCAAGTTGAAGATATTAAGTCAGCAGATTGGCAGGTTAAATTTATGCTGAACTTGTACCACATATGATCTCAATTTTCCTTCTTTTATCATTGTTGTGTTCTTTTTAGTTTTAATTTTTGAGTTACTTCCACTCAACTTATCCCCTTTCTCATTTCCGCATACATCTTCATGTGTGTGTATTTTAGGATATCATCCTTCAGAGTGAAGATTTCAGTATACAGGATATGGACATTTTTAATGGACATCTTGTGCTTTTTGTCAATAATAAGGGTGTTTCGATGTTATGTTCAATCAATTTGCCTTTAGATGCTAATTATAAGGTATGGAGGTTTGACTTTCTTGCCTTATTCTGTGACTAAATAAACATCAATATTACAGATGTTATATATCATTTCACATCTACTTGTCTGCAGCAACGATTGGAGATTAAGACACTTGACCCATGGTTTTTCCCTCTTCCCTCAAATTCCTGCAATGTAGCTCCAGGCTCAAACCATGACTTCATGAGCTCATTATACCGTGTGGTGCTTTCATCTCCAGTGGTACTTCATAATTTTACACACCTGCATAGAATTTCTTAGCATGTACTCTATCTGATTTTGTCTACTGAGCAGTTTTACTTTTATTCTCTCTTAATTCTGATTGTATTTTCGGTTTCCTTCGAATCATGATAGATTATTCTTCTCCGAAGCTCAGTTTTACCAAGTACAACCATGATAAATTTATGGGATCATTTGCTTCGTATGCTTCTAGTTATAAACTAGCTTAAAAATGCATTTCCCCACAGGTTTTAATCAAATTAGAATTTGAGAGTTTTTTCATCATAAATTTGCAATCATACAAGGTCATAATTAGAACTTAGAATAGAGTGTGAAGCTTAAATTTACCTGTCTCTGCTGCCCACACATCAAACCTTTCTAAGATTTCAATCTTCTCTTGGACCCACCAGATGCCAGATTTGATTGTTGACTATGACATGTCTAAACGGGTCTTCTCAATCATTCAGCAAGAGGAAGTACAAGTTAAGAATGATGTTAAACTTAAAACATATGTCCCAGATGAGTTGGATATAGAAGAAGTTTCAGATGCACAAAACAAAAGAGAGAACTTCCAGAATAGTGAATCCCAAATTTGGAAGGACTTTTCTGATGCATACTGTTGTGATAGGAAGGAAGTTATATCACATGACGGCACCAGAATACCCTTGACCATATTGTATTCTCCAATGACTTTTCAGAAAGGACAGTCACCTGGAGTTCTACAAGGGTATGGAGCATATGGTGAAATCCTGGATAAAAGTTGGTGTTCTAATCGCCTGAGTTTACTTGATCGTGGTTTTGTGCTGGCATTTGCAGACGTCAGGTAGATTCTATGAAAATAAAAGTTTCTTCATTTCATCTTTCCTTCATTGAAACTTAGTTTAGAACCTCAACTAGGAATTTAAGAATTTTTACATGGCAGGGGAGGAGGCGGTGTTGGTGATTCTTCATGGCATAGATGTGGAAGTGGGCTTGAGAAACAAAATTCAATACATGACTTTATCTCTTGTGCCAATTTCCTCACTGATAATGGTTATGTTCATAAGAATCGGCTTGGCTCCATTGGATACAGTGCAGGAGGTCTCCTTGTTGGGGCTGCTATCAACATGCATCCCGACCTGTTTCGAGCAGCCATTTTGAAGGTTAGCTTCAGCCATGGACTTGCCTATACACTGGTCTCTGGATCTAGTGAAGTAAATTGGAAATGCATCTTGTTTTATTTACTTCCTTTCATCTTCCAAAGAATCGGATGGACATGAATGGGTTCCTTAAACCATTCGATTAAATTCAATCTTAACTGATATTGAAATAAAATTTCAGTCCCTTTCTTTTACTTTCTAATCTCAGTGACATATTTGTAATCATTGTTAACCGATGGTTATAAATGGAGTGAATTAGGAAAGGTTATTATTACCGATATATTTTCTCTTTGATATTATAATACAGTTTCAGCCTCTCCAAAGACATTATACCATATATGTTAATGTCTTTAAATGTTCCGACTTTATTTAATGAAAAGGAAGAAAGCTCACTTTGTTATTCTCAGGTATTGTAAATGCTGTTATGAACTAAAGTTTTGCCAAAATGTATCAGGTTCCATTTCTTGACATATGCAACACGCTACTAGATCCAACTTTACCTCTCACCATTCTGGATTATGAAGAATTCGGAAACCCACAGATACGTGTGCAGTTCAAGTCCATTTTGAGCTATTCTCCCTACGATAACATATCTCGAAGAAGTTGTTATCCTTCTATGCTCGTCACTGCATCATTTCATGATGCAAGGTAAAATTTTATTTTTGTCTAACTACTTATACTGCTATCACATTAGCATTCACGTTTAAACATGTCAATCTTTGTCCATGTTTTGGTTTCATAAACTTATGCTGGAAATCATTGAACATTATTCTCAGGGTTGGAGTATGGGAAGCAGCCAAGTGGGTGGCAAAAATACGAGATACTACATGCTCTCATTGTTCGACTTCTGCAATTTTAAAGACCAATATGCTTGGAGGACATTTTGGTGAAGGTGGCCTCTACGGTGGATGTGAAGAGACAGCTTACGAGTACGCTTTTCTCATCAAAGTCCTCGGAACTTCTGACTGTGATTAAACTTTTGCACAATGTGATGAGAGAACTACAGTCTTCCAGTAACGGTAAGGAAGGGGGAATCTCTTGAAATTTATCTCCGAGTGTTTGTTTTTTAATAAGATCTTCAGGTGTTTGTTTGCTATTTAAGAAGTTAAATTGCTTTTGCTTCCATAAATATATAAAATCTGGTATCCAACTCTTAAGTTCCGCTTCTCTGTGTAAATCAAATTGATGTGGTAGTCCTCCTCACAAGTGCCCTTTTGCTAATTATTTCAGTTTTGGACTAGTAAATCACATTGGATGGATTATCTAGCCATGTGAAAATTGCTTATAAATTTCTTCAGTCTAGAGTGAACAAATTTTGTTCTTTTATCTTATATGTAGCCTCTAGGTAGACCGGAGCATTGAAGGTTAAAGAGCATACCATCCTTTAACCAAATCACGAACAGCTAATGCAAATAAGATGAAGACTTGCAGTATTTAATTTTAGCCCAGAAAAGCACAATTAACACATTCTGTTGACTTCATGATTATAGACAGCACAAACAATTAAATCAGTTGATGCTTTTCTTGCTGAATTGATGTGCTAATGATGTATTTAAATGACCATATCGGCATCGGCTATATCATATATGTCAAATATATTACTCTGACATTATATTGAACTGGAAAATCTTGAAGTAATGCTTGCTTAACAGGGGGTATTTGACCAAGGTCTTGCTTTGCTGCTGTAGAGATTGGCTACATGGATGAACTTCAAGCTTAATTGAACTTCACCTTGTGAGCAGAAGAGAAGTGCTTTGCTGCAGCTACCATGGCGGCCTCTGGCCCAGTTGGGGATGCTGCAGCCTTGGATGTCTCTGCATTTCCACCATCAGAGAAGAGTAAGCAATAAATGAAGGACTTTATACTTGACCAATACAAATTCTCTTTCTTCTTCCCTTCCATCTTTTTAGGACCTCTGAGAGAAAAAGAAACGAACAACTGAAGAAGAAAGGCTTTTGGGAAGTAGAGAACAAAGGGGGATTATGGATCAGAAATTATTGAGAATTTGGTTGAAATGTTTGTTGTTAAGGTGAGTGAAAACTTGTGTGTGATCTTCCCTATTTATAGGCAGAGGAAATGAGGCTCCTTGACAATCAAGAAAAACATGTCATTGTCAACATTGACAATGTGATTATTCAGACATTTTTTTAACAAGGTTCATCTTTCTTTGTGTTGATTGGAAACTACTCTTCTTGACTTAGCTGCCTTTGAATGGTCAATATATTAAAGTGAAATCATG

mRNA sequence

ATGGCTCTTAAATCCCTGCTAAAACCCAAATGCTCCATTAGAAAATTCTTCCTTTCTTCTTCCTCCATATCGGTTTCGCGATTCTCGTCTCTCTGCAAAGAACGCATCTTCTCACTGCCTTCCGAATCCCCACCAGCTGCTAAAAAAGTCCCTTTCACTCACTCAGTTCATGGCGTTACGCTGCAAGATCCCTACCATTGGATGTCCAACACCGACGACCCTGATCTCGCCGACTACCTCCGTCGAGAAAACTTGTACGCTGAATCTTTCATGGCCGATACTCAGATTCTGCAGCGGCGGCTCTTCTCCGAGATGACGAGTCGAATGCCCGCCAAGGTTTCCACTCCTCCTGAGCCTTGGGGACCCTGGTTTTACTACCAGTACATTCCGGAGGGGAAGGATTACCCAGTTCTATGTCGTAGGTTACAGATTGAGAAAGGCGGTTGGTTAAAGAAATTTGTACAATTTGCCAAAGGAAATTTTGGGAGGGAGGAGGAAGTTTTGATTGATTGGAATGAAATTGCTAAACAATATGTTATGAAAATTATGGCATTCATTAATTTCTACAATGAGTTGCTCATGATTGTAGGCTATGTTCACGTGGGAACTTGCCGTGTTTCACCAGACCACAACTTTCTGGCATACACAGTTGATATTACTGGCAGTGAACACTTCATGCTTCAGGTTAAAGACCTCAGAACTGGACTGATAATTTCCAAGTTACAGGAGGGAGTTTCTGAAACTCTGCTGGTTTGTCTAACTCAGGTTTATATTATAGATGCTAACCACTCATTAAGTGGTTTGCAAAGAATACACAAACGCATTCCTGGTATTCAGTACTTTCTGGAACATCATAGTGGTTTCTTTTATATCCTAACAAATGCTCCGCTAGAAAAGAATGGAGATTGTTCCAAGGAAGAGTATTATGTAGCTCGATGTCAAGTTGAAGATATTAAGTCAGCAGATTGGCAGGATATCATCCTTCAGAGTGAAGATTTCAGTATACAGGATATGGACATTTTTAATGGACATCTTGTGCTTTTTGTCAATAATAAGGGTGTTTCGATGTTATGTTCAATCAATTTGCCTTTAGATGCTAATTATAAGCAACGATTGGAGATTAAGACACTTGACCCATGGTTTTTCCCTCTTCCCTCAAATTCCTGCAATGTAGCTCCAGGCTCAAACCATGACTTCATGAGCTCATTATACCGTGTGGTGCTTTCATCTCCAGTGATGCCAGATTTGATTGTTGACTATGACATGTCTAAACGGGTCTTCTCAATCATTCAGCAAGAGGAAGTACAAGTTAAGAATGATGTTAAACTTAAAACATATGTCCCAGATGAGTTGGATATAGAAGAAGTTTCAGATGCACAAAACAAAAGAGAGAACTTCCAGAATAGTGAATCCCAAATTTGGAAGGACTTTTCTGATGCATACTGTTGTGATAGGAAGGAAGTTATATCACATGACGGCACCAGAATACCCTTGACCATATTGTATTCTCCAATGACTTTTCAGAAAGGACAGTCACCTGGAGTTCTACAAGGGTATGGAGCATATGGTGAAATCCTGGATAAAAGTTGGTGTTCTAATCGCCTGAGTTTACTTGATCGTGGTTTTGTGCTGGCATTTGCAGACGTCAGGGGAGGAGGCGGTGTTGGTGATTCTTCATGGCATAGATGTGGAAGTGGGCTTGAGAAACAAAATTCAATACATGACTTTATCTCTTGTGCCAATTTCCTCACTGATAATGGTTATGTTCATAAGAATCGGCTTGGCTCCATTGGATACAGTGCAGGAGGTCTCCTTGTTGGGGCTGCTATCAACATGCATCCCGACCTGTTTCGAGCAGCCATTTTGAAGGTTAGCTTCAGCCATGGACTTGCCTATACACTGGTCTCTGGATCTAGTGAAGTTCCATTTCTTGACATATGCAACACGCTACTAGATCCAACTTTACCTCTCACCATTCTGGATTATGAAGAATTCGGAAACCCACAGATACGTGTGCAGTTCAAGTCCATTTTGAGCTATTCTCCCTACGATAACATATCTCGAAGAAGTTGTTATCCTTCTATGCTCGTCACTGCATCATTTCATGATGCAAGGGTTGGAGTATGGGAAGCAGCCAAGTGGGTGGCAAAAATACGAGATACTACATGCTCTCATTGTTCGACTTCTGCAATTTTAAAGACCAATATGCTTGGAGGACATTTTGGTGAAGGTGGCCTCTACGGTGGATGTGAAGAGACAGCTTACGAGTACGCTTTTCTCATCAAAGTCCTCGGAACTTCTGACTGTGATTAA

Coding sequence (CDS)

ATGGCTCTTAAATCCCTGCTAAAACCCAAATGCTCCATTAGAAAATTCTTCCTTTCTTCTTCCTCCATATCGGTTTCGCGATTCTCGTCTCTCTGCAAAGAACGCATCTTCTCACTGCCTTCCGAATCCCCACCAGCTGCTAAAAAAGTCCCTTTCACTCACTCAGTTCATGGCGTTACGCTGCAAGATCCCTACCATTGGATGTCCAACACCGACGACCCTGATCTCGCCGACTACCTCCGTCGAGAAAACTTGTACGCTGAATCTTTCATGGCCGATACTCAGATTCTGCAGCGGCGGCTCTTCTCCGAGATGACGAGTCGAATGCCCGCCAAGGTTTCCACTCCTCCTGAGCCTTGGGGACCCTGGTTTTACTACCAGTACATTCCGGAGGGGAAGGATTACCCAGTTCTATGTCGTAGGTTACAGATTGAGAAAGGCGGTTGGTTAAAGAAATTTGTACAATTTGCCAAAGGAAATTTTGGGAGGGAGGAGGAAGTTTTGATTGATTGGAATGAAATTGCTAAACAATATGTTATGAAAATTATGGCATTCATTAATTTCTACAATGAGTTGCTCATGATTGTAGGCTATGTTCACGTGGGAACTTGCCGTGTTTCACCAGACCACAACTTTCTGGCATACACAGTTGATATTACTGGCAGTGAACACTTCATGCTTCAGGTTAAAGACCTCAGAACTGGACTGATAATTTCCAAGTTACAGGAGGGAGTTTCTGAAACTCTGCTGGTTTGTCTAACTCAGGTTTATATTATAGATGCTAACCACTCATTAAGTGGTTTGCAAAGAATACACAAACGCATTCCTGGTATTCAGTACTTTCTGGAACATCATAGTGGTTTCTTTTATATCCTAACAAATGCTCCGCTAGAAAAGAATGGAGATTGTTCCAAGGAAGAGTATTATGTAGCTCGATGTCAAGTTGAAGATATTAAGTCAGCAGATTGGCAGGATATCATCCTTCAGAGTGAAGATTTCAGTATACAGGATATGGACATTTTTAATGGACATCTTGTGCTTTTTGTCAATAATAAGGGTGTTTCGATGTTATGTTCAATCAATTTGCCTTTAGATGCTAATTATAAGCAACGATTGGAGATTAAGACACTTGACCCATGGTTTTTCCCTCTTCCCTCAAATTCCTGCAATGTAGCTCCAGGCTCAAACCATGACTTCATGAGCTCATTATACCGTGTGGTGCTTTCATCTCCAGTGATGCCAGATTTGATTGTTGACTATGACATGTCTAAACGGGTCTTCTCAATCATTCAGCAAGAGGAAGTACAAGTTAAGAATGATGTTAAACTTAAAACATATGTCCCAGATGAGTTGGATATAGAAGAAGTTTCAGATGCACAAAACAAAAGAGAGAACTTCCAGAATAGTGAATCCCAAATTTGGAAGGACTTTTCTGATGCATACTGTTGTGATAGGAAGGAAGTTATATCACATGACGGCACCAGAATACCCTTGACCATATTGTATTCTCCAATGACTTTTCAGAAAGGACAGTCACCTGGAGTTCTACAAGGGTATGGAGCATATGGTGAAATCCTGGATAAAAGTTGGTGTTCTAATCGCCTGAGTTTACTTGATCGTGGTTTTGTGCTGGCATTTGCAGACGTCAGGGGAGGAGGCGGTGTTGGTGATTCTTCATGGCATAGATGTGGAAGTGGGCTTGAGAAACAAAATTCAATACATGACTTTATCTCTTGTGCCAATTTCCTCACTGATAATGGTTATGTTCATAAGAATCGGCTTGGCTCCATTGGATACAGTGCAGGAGGTCTCCTTGTTGGGGCTGCTATCAACATGCATCCCGACCTGTTTCGAGCAGCCATTTTGAAGGTTAGCTTCAGCCATGGACTTGCCTATACACTGGTCTCTGGATCTAGTGAAGTTCCATTTCTTGACATATGCAACACGCTACTAGATCCAACTTTACCTCTCACCATTCTGGATTATGAAGAATTCGGAAACCCACAGATACGTGTGCAGTTCAAGTCCATTTTGAGCTATTCTCCCTACGATAACATATCTCGAAGAAGTTGTTATCCTTCTATGCTCGTCACTGCATCATTTCATGATGCAAGGGTTGGAGTATGGGAAGCAGCCAAGTGGGTGGCAAAAATACGAGATACTACATGCTCTCATTGTTCGACTTCTGCAATTTTAAAGACCAATATGCTTGGAGGACATTTTGGTGAAGGTGGCCTCTACGGTGGATGTGAAGAGACAGCTTACGAGTACGCTTTTCTCATCAAAGTCCTCGGAACTTCTGACTGTGATTAA

Protein sequence

MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVHGVTLQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPEPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQYVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLIISKLQEGVSETLLVCLTQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLGTSDCD
Homology
BLAST of Spg010295 vs. NCBI nr
Match: XP_022977225.1 (uncharacterized protein LOC111477597 [Cucurbita maxima])

HSP 1 Score: 1265.4 bits (3273), Expect = 0.0e+00
Identity = 636/834 (76.26%), Postives = 684/834 (82.01%), Query Frame = 0

Query: 1   MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVHGVT 60
           MALKSLLKPKC  RK  LSSS  S S FSSLCKERIFSLPSESPP AKKVPFTHSVHG+T
Sbjct: 1   MALKSLLKPKCFTRKAILSSSLASSSLFSSLCKERIFSLPSESPPTAKKVPFTHSVHGIT 60

Query: 61  LQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPEPW 120
           LQDPYHWM+NT DPDLADYLRRENLYAE+FMADTQILQRRLFSEMTSR+P KVSTPPEPW
Sbjct: 61  LQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRIPTKVSTPPEPW 120

Query: 121 GPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQYVM 180
           GPWFYYQYIPEGK+YPVLCRRL  +K  WLKK  QFAKGN G++EEVL+DWNEIAKQY  
Sbjct: 121 GPWFYYQYIPEGKEYPVLCRRLLNQKTNWLKKLTQFAKGNSGKQEEVLLDWNEIAKQY-- 180

Query: 181 KIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLIISK 240
                           GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDLR+GL+I K
Sbjct: 181 ----------------GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPK 240

Query: 241 LQEGV------------------------------------SETLLV--------CL--- 300
           LQEGV                                     E +LV        C+   
Sbjct: 241 LQEGVVSLAWAEEGRTLFYTQADENQRPYRVFSTKLGFSNTEEDVLVFVENDPNYCVDIT 300

Query: 301 -----------------TQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTNAP 360
                            ++VYIIDAN+SLSGLQRIHKRIPGIQYFLEHH GFFYILTNAP
Sbjct: 301 STKDGKFITVNSNSRTSSEVYIIDANNSLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAP 360

Query: 361 LEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGVSM 420
           LEK GDC KE+YYVARC+VEDIKSA+WQDI+LQS+DFSIQDMD+F+GHLVLFVN  GV M
Sbjct: 361 LEKKGDCLKEDYYVARCRVEDIKSANWQDIVLQSQDFSIQDMDVFSGHLVLFVNKNGVPM 420

Query: 421 LCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDL 480
           LCSINLPLDAN+K  LEI+ LDPWFFPLPSNSC+V+PGSNHDFMSSLYRVVLSSP+MPDL
Sbjct: 421 LCSINLPLDANHKHCLEIEKLDPWFFPLPSNSCSVSPGSNHDFMSSLYRVVLSSPLMPDL 480

Query: 481 IVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKD 540
           IVDYDMSKRVFSIIQQEEV+VK+DVKLKTY P+ L IE+VSDAQNKRENF+N ES+ WKD
Sbjct: 481 IVDYDMSKRVFSIIQQEEVEVKHDVKLKTYQPNALGIEKVSDAQNKRENFENRESKTWKD 540

Query: 541 FSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLS 600
           FSD+YCC+RKEVISHDG R+PLTILYSP TFQKG+S GVLQGYGAYGE+LDKSWC +RLS
Sbjct: 541 FSDSYCCERKEVISHDGIRVPLTILYSPSTFQKGRSLGVLQGYGAYGEVLDKSWCPSRLS 600

Query: 601 LLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGS 660
           LLDRGFVLAFAD+RGGGG GDSSWHR GSGLEKQNSI DFI CANFL DNGYVHKNRL S
Sbjct: 601 LLDRGFVLAFADIRGGGG-GDSSWHRSGSGLEKQNSIQDFIFCANFLIDNGYVHKNRLAS 660

Query: 661 IGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLP 720
           IGYSAGGLLVGAAINMHPDLFRAAILK                 VPFLDICNTLLDP+LP
Sbjct: 661 IGYSAGGLLVGAAINMHPDLFRAAILK-----------------VPFLDICNTLLDPSLP 720

Query: 721 LTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVA 771
           LTILDYEEFGNPQI +QF+SILSYSPYDNIS+ SCYP MLVTASF DARVGVWEAAKWVA
Sbjct: 721 LTILDYEEFGNPQIAMQFESILSYSPYDNISKGSCYPPMLVTASFRDARVGVWEAAKWVA 780

BLAST of Spg010295 vs. NCBI nr
Match: XP_023550805.1 (uncharacterized protein LOC111808835, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1265.0 bits (3272), Expect = 0.0e+00
Identity = 636/834 (76.26%), Postives = 684/834 (82.01%), Query Frame = 0

Query: 1   MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVHGVT 60
           MALKSLLKPKC  RK  LSSS  S S FSSLCKERIFSLPSESPPAAKKVPFTHSVHG+T
Sbjct: 10  MALKSLLKPKCFTRKAILSSSIASSSLFSSLCKERIFSLPSESPPAAKKVPFTHSVHGIT 69

Query: 61  LQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPEPW 120
           LQDPYHWM+NT DPDLAD+LRRENLYA++FMADTQILQRRLFSEMTSR+P KVSTPPEPW
Sbjct: 70  LQDPYHWMANTADPDLADFLRRENLYADAFMADTQILQRRLFSEMTSRIPTKVSTPPEPW 129

Query: 121 GPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQYVM 180
           GPWFYYQYIPEGK+YPVLCRRLQ EK  WLKK  QFAKGN G++EEVL+DWNEIAKQY  
Sbjct: 130 GPWFYYQYIPEGKEYPVLCRRLQNEKTNWLKKLTQFAKGNSGKQEEVLLDWNEIAKQY-- 189

Query: 181 KIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLIISK 240
                           GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDLR+GL+I K
Sbjct: 190 ----------------GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPK 249

Query: 241 LQEGV------------------------------------SETLLV--------CL--- 300
           LQEGV                                     E +LV        C+   
Sbjct: 250 LQEGVVSLAWAEEGRTLFYTQADENQRPYRVFSTKLGFSDTEEDVLVFVENDPNYCVDIT 309

Query: 301 -----------------TQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTNAP 360
                            ++VYIIDAN+SLSGLQRIHKRIPGIQYFLEHH GFFYILTNAP
Sbjct: 310 STKDGKFITVNSNSRTSSEVYIIDANNSLSGLQRIHKRIPGIQYFLEHHRGFFYILTNAP 369

Query: 361 LEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGVSM 420
           LEK GDCSKE+YYVA+C+VEDIKSA+WQD +LQSEDFSIQDMD+F+GHLVLFVN  GV M
Sbjct: 370 LEKKGDCSKEDYYVAQCRVEDIKSANWQDTVLQSEDFSIQDMDVFSGHLVLFVNKNGVPM 429

Query: 421 LCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDL 480
           LCSINLPLDAN+K RLEI+ LDPWFFPLPSNSC+VAPGSNHDF SSLYRVVLSSPVMPDL
Sbjct: 430 LCSINLPLDANHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFTSSLYRVVLSSPVMPDL 489

Query: 481 IVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKD 540
           IVDYDMSKRVFSIIQQEEV+VK+DVKLKTY PD  DIE+VS  QNKRENF+  ES+ WKD
Sbjct: 490 IVDYDMSKRVFSIIQQEEVEVKHDVKLKTYQPDASDIEKVS--QNKRENFETRESETWKD 549

Query: 541 FSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLS 600
           FSD+YCC+RKEVISHDG R+PLTILYSP TFQKG+SPGVLQGYGAYGE+LDKSWC +RLS
Sbjct: 550 FSDSYCCERKEVISHDGIRVPLTILYSPSTFQKGRSPGVLQGYGAYGEVLDKSWCPSRLS 609

Query: 601 LLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGS 660
           LLDRGFVLAFAD+RGGGG GDSSWHRCGSGLEKQNSI DFI CANFL DNGYVHK+RLGS
Sbjct: 610 LLDRGFVLAFADIRGGGG-GDSSWHRCGSGLEKQNSIQDFIFCANFLIDNGYVHKDRLGS 669

Query: 661 IGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLP 720
           IGYSAGGLLVGAAINMHPDLF AAILK                 VPFLDICNTLLDP+LP
Sbjct: 670 IGYSAGGLLVGAAINMHPDLFGAAILK-----------------VPFLDICNTLLDPSLP 729

Query: 721 LTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVA 771
           LTILDYEEFGNP+I +QF+SILSYSPYDNIS+ SCYP MLVTASF DARVGVWEAAKWVA
Sbjct: 730 LTILDYEEFGNPEIAMQFESILSYSPYDNISKGSCYPPMLVTASFRDARVGVWEAAKWVA 789

BLAST of Spg010295 vs. NCBI nr
Match: XP_022957328.1 (uncharacterized protein LOC111458759 [Cucurbita moschata])

HSP 1 Score: 1258.8 bits (3256), Expect = 0.0e+00
Identity = 634/834 (76.02%), Postives = 681/834 (81.65%), Query Frame = 0

Query: 1   MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVHGVT 60
           MALKSLLKP    R   L SS  S S FSSLCKERIFSLPSESPPAAKKVPFTHSVHG+T
Sbjct: 1   MALKSLLKP---TRNSILCSSISSSSLFSSLCKERIFSLPSESPPAAKKVPFTHSVHGIT 60

Query: 61  LQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPEPW 120
           LQDPYHWM+NT DPDLADYLRRENLYAE+FMADTQILQRRLFSEMTSR+  KVSTPPEPW
Sbjct: 61  LQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRISTKVSTPPEPW 120

Query: 121 GPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQYVM 180
           GPWFYYQYIPEGK+YPVLCRRLQ EK  WLKK  QFAKGN G++EEVL+DWNEIAKQY  
Sbjct: 121 GPWFYYQYIPEGKEYPVLCRRLQNEKTNWLKKLTQFAKGNSGKQEEVLLDWNEIAKQY-- 180

Query: 181 KIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLIISK 240
                           GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDLR+GL+I K
Sbjct: 181 ----------------GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPK 240

Query: 241 LQEGV------------------------------------SETLLV--------CL--- 300
           LQEGV                                     E +LV        C+   
Sbjct: 241 LQEGVVSLAWAEEGRTLFYTQADENQRPYRVFSTKLGFSDTGEDVLVFVENDPNYCVDIT 300

Query: 301 -----------------TQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTNAP 360
                            ++VYIIDAN+ LSGLQRIHKRIPGIQYFLEHH GFFYILTNAP
Sbjct: 301 STKDGKFITVNSNSRTSSEVYIIDANNWLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAP 360

Query: 361 LEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGVSM 420
           LEK GDCSKE+YYVARC+VEDIKSA+WQDI+LQS+DFSI DMD+F+GHLVLFVN  GV M
Sbjct: 361 LEKKGDCSKEDYYVARCRVEDIKSANWQDIVLQSKDFSIHDMDVFSGHLVLFVNKNGVPM 420

Query: 421 LCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDL 480
           LCSINLPLDAN+K RLEI+ LDPWFFPLPSNSC+VAPGSNHDF SSLYRVVLSSPVMPDL
Sbjct: 421 LCSINLPLDANHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFTSSLYRVVLSSPVMPDL 480

Query: 481 IVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKD 540
           IVDYDMSKRVFSIIQQEEV+VK+D+KLKTY PD L IE+VSDAQNKRENF+  ES+ WKD
Sbjct: 481 IVDYDMSKRVFSIIQQEEVEVKHDIKLKTYQPDALGIEKVSDAQNKRENFETRESETWKD 540

Query: 541 FSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLS 600
           FSD+YCC+RKEVISHDG R+PLTILYSP TFQKG+SPGVLQGYGAYGE+LDKSWC +RLS
Sbjct: 541 FSDSYCCERKEVISHDGIRVPLTILYSPSTFQKGRSPGVLQGYGAYGEVLDKSWCPSRLS 600

Query: 601 LLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGS 660
           LLDRGFVLAFAD+RGGGG GDSSWHRCGSGL+KQNSI DFI CANFL DNGYVHKNRLGS
Sbjct: 601 LLDRGFVLAFADIRGGGG-GDSSWHRCGSGLQKQNSIQDFIFCANFLIDNGYVHKNRLGS 660

Query: 661 IGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLP 720
           IGYSAGGLLVGAAINMHPDLF AAILK                 VPFLDICNTLLDP+LP
Sbjct: 661 IGYSAGGLLVGAAINMHPDLFGAAILK-----------------VPFLDICNTLLDPSLP 720

Query: 721 LTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVA 771
           LTILDYEEFGNP+I +QF+SILSYSPYDNIS+ SCYP MLVTASF DARVGVWEAAKWVA
Sbjct: 721 LTILDYEEFGNPEIAMQFESILSYSPYDNISKGSCYPPMLVTASFRDARVGVWEAAKWVA 780

BLAST of Spg010295 vs. NCBI nr
Match: XP_022149039.1 (uncharacterized protein LOC111017556 [Momordica charantia])

HSP 1 Score: 1258.0 bits (3254), Expect = 0.0e+00
Identity = 644/835 (77.13%), Postives = 682/835 (81.68%), Query Frame = 0

Query: 1   MALKSLL--KPKCSIRKFFL-SSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVH 60
           MALKSLL  KPKCSI+K  L SSSS S S FSS C++R FSLPSESPPAAKKVPF +SVH
Sbjct: 17  MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 76

Query: 61  GVTLQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           GVTLQDP+HWMSNTDDPDLADYLRRENLYAE+FMADTQILQRRLFSEMTSRMPAKVSTPP
Sbjct: 77  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 136

Query: 121 EPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGR-EEEVLIDWNEIAK 180
           EPWGPWFYYQYIP GK+YPVLCRRLQ EK GWLKK VQFA+GNFG+ EEEVL+DWNEIAK
Sbjct: 137 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 196

Query: 181 QYVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGL 240
            Y                  GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDL +GL
Sbjct: 197 HY------------------GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGL 256

Query: 241 IISKLQEGV--------SETLL-----------------------------------VCL 300
           II K Q+GV          TL                                     C+
Sbjct: 257 IIPKSQKGVVSLAWAEEGRTLFYTQSDENQRPYRVFCTKVGCSDAEDVSVFVENDPNFCV 316

Query: 301 --------------------TQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILT 360
                               ++VYIIDAN+ LSGLQRIHKRIPGIQYFLEHH GFFYILT
Sbjct: 317 DVTSTKDGKFITVNSNSRTSSEVYIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILT 376

Query: 361 NAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKG 420
           NAPLEKNGDCSKEEYYVARC+VEDIKS+DWQD ILQSEDFSIQDMDIF+GHLVLFVN  G
Sbjct: 377 NAPLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMG 436

Query: 421 VSMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVM 480
           VSMLC+INLPLD N+K RLEI+ LDPWFFPLPSNSC+VAPGSNHDFMSSLYRVVLSSPVM
Sbjct: 437 VSMLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVM 496

Query: 481 PDLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQI 540
           PDL+VDYDMSKRVFSIIQQEEVQVK+DV+LKT +PDELD+EEVS A+NK  NFQNSESQI
Sbjct: 497 PDLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQI 556

Query: 541 WKDFSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSN 600
            KDFSDAYCC+RKEVISHDG RIPLTILYSP+ F KG+SPGVL GYGAYGEILDKSWC  
Sbjct: 557 SKDFSDAYCCERKEVISHDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPY 616

Query: 601 RLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNR 660
           RLSLLDRGFVLAFADVRGGGG GDSSWHR GSGLEKQNSIHDFISCA FL DN YVHKN+
Sbjct: 617 RLSLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQ 676

Query: 661 LGSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDP 720
           LGSIGYSAGGLLVGAAINM PDLFRAAILK                 VPFLDICNTLLDP
Sbjct: 677 LGSIGYSAGGLLVGAAINMRPDLFRAAILK-----------------VPFLDICNTLLDP 736

Query: 721 TLPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAK 769
           +LPLTILDYEEFGNPQ+  QF+SIL+YSPYDNISR SCYP MLVT+SF DARVGVWEAAK
Sbjct: 737 SLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRGSCYPPMLVTSSFRDARVGVWEAAK 796

BLAST of Spg010295 vs. NCBI nr
Match: KAG6601360.1 (Protease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1255.4 bits (3247), Expect = 0.0e+00
Identity = 628/801 (78.40%), Postives = 675/801 (84.27%), Query Frame = 0

Query: 1   MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVHGVT 60
           MALKSLLKP    RK  L SS  S S FSSLCKERIFSLPSESPP AKKVPFTHSVHG+T
Sbjct: 1   MALKSLLKP---TRKSILCSSISSSSLFSSLCKERIFSLPSESPPTAKKVPFTHSVHGIT 60

Query: 61  LQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPEPW 120
           LQDPYHWM+NT DPDLADYLRRENLYAE+FMADTQILQRRLFSEMTSR+P KVSTPPEPW
Sbjct: 61  LQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRIPTKVSTPPEPW 120

Query: 121 GPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQYVM 180
           GPWFYYQYIPEGK+YPVLCRRLQ EK  WLKK  QFAKGN G++EEVL+DWNEIAKQY  
Sbjct: 121 GPWFYYQYIPEGKEYPVLCRRLQNEKTNWLKKLTQFAKGNSGKQEEVLLDWNEIAKQY-- 180

Query: 181 KIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLIISK 240
                           GYVHVGTCRVSPDHNFLA TVDITGSEHFMLQ+KDLR+GL+I K
Sbjct: 181 ----------------GYVHVGTCRVSPDHNFLACTVDITGSEHFMLQIKDLRSGLMIPK 240

Query: 241 L---QEGVSETLLVCLTQV-----YII-----------------------DANHSLSGLQ 300
           +   + G S+T    L  V     Y +                       DAN+ LSGLQ
Sbjct: 241 VFSTKLGFSDTEEDVLVFVENDPNYCVDITSTKDGKFITVNSNSRTSSEEDANNLLSGLQ 300

Query: 301 RIHKRIPGIQYFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQ 360
           RIHKRIPGIQYFLEHH GFFYILTNAPLEK GDCSKE+YYVARC+VEDIKSA+WQDI+LQ
Sbjct: 301 RIHKRIPGIQYFLEHHHGFFYILTNAPLEKKGDCSKEDYYVARCRVEDIKSANWQDIVLQ 360

Query: 361 SEDFSIQDMDIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSC 420
           SEDFSIQDMD+F+GHLVLFVN  GV MLCSINLPLDAN+K RLEI+ LDPWFFPLPSNSC
Sbjct: 361 SEDFSIQDMDVFSGHLVLFVNKNGVPMLCSINLPLDANHKHRLEIEKLDPWFFPLPSNSC 420

Query: 421 NVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPD 480
           ++APGSNHDF SSLYRVVLSS VMPDLIVDYDMSKRVFSIIQQEEV+VK+DVKLKTY PD
Sbjct: 421 SIAPGSNHDFTSSLYRVVLSSAVMPDLIVDYDMSKRVFSIIQQEEVEVKHDVKLKTYQPD 480

Query: 481 ELDIEEVSDAQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDGTRIPLTILYSPMTFQK 540
            LD+E+VSDAQNKRENF+  ES+ WKDFSD+YCC+RKEVISHDG R+PLTILYSP TFQK
Sbjct: 481 ALDVEKVSDAQNKRENFETRESETWKDFSDSYCCERKEVISHDGIRVPLTILYSPSTFQK 540

Query: 541 GQSPGVLQGYGAYGEILDKSWCSNRLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEK 600
           G+SPGVLQGYGAYGE+LDKSWC +RLSLLDRGFVLAFAD+RGGGG GDSSWHRCGSGL+K
Sbjct: 541 GRSPGVLQGYGAYGEVLDKSWCPSRLSLLDRGFVLAFADIRGGGG-GDSSWHRCGSGLQK 600

Query: 601 QNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHG 660
           QNSI DFI CANFL DNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLF AAILK      
Sbjct: 601 QNSIQDFIFCANFLIDNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFGAAILK------ 660

Query: 661 LAYTLVSGSSEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRR 720
                      VPFLDICNTLLDP+LPLTILDYEEFGNP+I +QF+SILSYSPYDNIS+ 
Sbjct: 661 -----------VPFLDICNTLLDPSLPLTILDYEEFGNPEIAMQFESILSYSPYDNISKG 720

Query: 721 SCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGG 771
           SCYP MLVTASF DARVGVWEAAKWVAKIRDTTCS CSTSAILKTNM+GGHFGEGGLYGG
Sbjct: 721 SCYPPMLVTASFRDARVGVWEAAKWVAKIRDTTCSRCSTSAILKTNMVGGHFGEGGLYGG 762

BLAST of Spg010295 vs. ExPASy Swiss-Prot
Match: Q59536 (Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.7e-71
Identity = 205/787 (26.05%), Postives = 332/787 (42.19%), Query Frame = 0

Query: 45  PAAKKVPFTHSVHGVTLQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSE 104
           P AK++P  H +HG   +D Y+W+ + D+ ++  YL  EN Y    M   Q    +++  
Sbjct: 4   PIAKRIPHPHELHGDVREDDYYWLKDRDNTEVIQYLEEENRYYHEIMRPLQEQTEQIYES 63

Query: 105 MTSRMPAKVSTPPEPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGRE 164
           M  R+P      P   G +FYY  + + K YP+  R+    +       +Q A       
Sbjct: 64  MVDRVPDSEMKVPVQHGQFFYYSRLDKNKQYPIYARKQAASRA-----LLQDA------T 123

Query: 165 EEVLIDWNEIAKQYVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEH 224
           EEV++D NE+A++                    Y+ V   R++ DH+ LAY  +  G++ 
Sbjct: 124 EEVVLDLNELAEE------------------DDYLSVTVQRMTTDHSRLAYLENRDGTDR 183

Query: 225 FMLQVKDLRTG------------------------------------------------- 284
           + + +KDL TG                                                 
Sbjct: 184 YTIYIKDLNTGELLSDRVPNVYIYGSMEWCRCGDYIFYTTVDEHQRPCQLWRHRLGSDVE 243

Query: 285 --------------LIISKLQEG---VSETLLVCLTQVYIIDANHSLSGLQRIHKRIPGI 344
                         L ISK Q G      +     +++++ID +  LS LQ + +R  GI
Sbjct: 244 SDELIFEEKDDTFTLFISKSQSGKFIFVYSSSKTTSEIHMIDTDSPLSPLQLVDERRDGI 303

Query: 345 QYFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDM 404
            Y +EH      ILTN             + + RC + D+ S    +++  +E+  +Q+M
Sbjct: 304 LYDVEHWEDDLLILTNE--------GALNFQLLRCPLNDLSSK--VNVVEYNEERYLQEM 363

Query: 405 DIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHD 464
             F   L++     G++ +  ++   D   +Q         W  P               
Sbjct: 364 YPFRDKLLIAGRENGLTQIWVVH---DGELQQ-------ISWDEP--------------- 423

Query: 465 FMSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSD 524
               LY V + S         YD ++    +IQ E +              E    +V+ 
Sbjct: 424 ----LYTVAVLSE------QSYDTNE---VLIQYESLLTPKTTFGLNLQTGEKQCLQVAP 483

Query: 525 AQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQG 584
              + +  Q  + Q+W                  G ++P+T +Y       G +P +L G
Sbjct: 484 VSGEYDRSQFRQEQLW-------------ATGRSGVKVPMTAVYLEGALDNGPAPLILYG 543

Query: 585 YGAYGEILDKSWCSNRLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFIS 644
           YG+YG   D  +   RL LL++G V   A VRGG  +G   W+  G    K+N+  DFI+
Sbjct: 544 YGSYGSNSDPRFDPYRLPLLEKGIVFVTAQVRGGSEMG-RGWYEDGKMQNKRNTFTDFIA 603

Query: 645 CANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGS 704
            A  L D  Y    ++ + G SAGGLLVGA  NM  +LF+  +                 
Sbjct: 604 AAKHLIDQNYTSPTKMAARGGSAGGLLVGAVANMAGELFKVIV----------------- 663

Query: 705 SEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVT 764
             VPF+D+  T+LD ++PLT L+++E+G+P+ +  +  + SYSPYDN+  +  YP M +T
Sbjct: 664 PAVPFVDVVTTMLDTSIPLTTLEWDEWGDPRKQEDYFYMKSYSPYDNVEAKD-YPHMYIT 679

Query: 765 ASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYA 766
              +D RVG +E AKWVA++R       + + ++KTNM  GHFG+ G +   +E A  YA
Sbjct: 724 TGINDPRVGYFEPAKWVARLRAVKTD--NNTLVMKTNMGAGHFGKSGRFNHLKEAAESYA 679

BLAST of Spg010295 vs. ExPASy Swiss-Prot
Match: P24555 (Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 3.0e-65
Identity = 202/785 (25.73%), Postives = 339/785 (43.18%), Query Frame = 0

Query: 45  PAAKKVPFTHSVHGVTLQDPYHWM--SNTDDPDLADYLRRENLYAESFMADTQILQRRLF 104
           P A ++P   ++HG T  D Y+W+       P++ DYL++EN Y    MA  Q LQ R+ 
Sbjct: 3   PKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDRIL 62

Query: 105 SEMTSRMPAKVSTPPEPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFG 164
            E+  R+P +  + P     + Y      G +Y +  R              Q A     
Sbjct: 63  KEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQR--------------QSAFSEEW 122

Query: 165 REEEVLIDWNEIAKQYVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGS 224
            E E L+D N+ A        A   FY+          +G   ++PD+  +A   D    
Sbjct: 123 DEWETLLDANKRA--------AHSEFYS----------MGGMAITPDNTIMALAEDFLSR 182

Query: 225 EHFMLQVKDLRTGLIISKLQEGV------------------------------------- 284
             + ++ ++L TG    +L + V                                     
Sbjct: 183 RQYGIRFRNLETGNWYPELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPA 242

Query: 285 ----------SETLLVCL------------------TQVYIIDANHSLSGLQRIHKRIPG 344
                      +T  V L                  ++V ++DA  + +       R   
Sbjct: 243 SQDKLIYEEKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKD 302

Query: 345 IQYFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQD 404
            +Y L+H+   FY+ +N    ++G    + + + R ++ D     W+++I   E+  ++ 
Sbjct: 303 HEYSLDHYQHRFYLRSN----RHG----KNFGLYRTRMRD--EQQWEELIPPRENIMLEG 362

Query: 405 MDIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNH 464
             +F   LV+    +G++ L  IN       ++ + I   DP +    +         N 
Sbjct: 363 FTLFTDWLVVEERQRGLTSLRQIN----RKTREVIGIAFDDPAYVTWIA--------YNP 422

Query: 465 DFMSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVS 524
           +  ++  R   SS   PD + + DM      +++Q EV                      
Sbjct: 423 EPETARLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVP--------------------- 482

Query: 525 DAQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQ 584
                              ++  Y  +   +++ DG  +P++++Y    F+KG +P ++ 
Sbjct: 483 -----------------GFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY 542

Query: 585 GYGAYGEILDKSWCSNRLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFI 644
           GYG+YG  +D  +  +RLSLLDRGFV A   VRGGG +G   W+  G  L+K+N+ +D++
Sbjct: 543 GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG-QQWYEDGKFLKKKNTFNDYL 602

Query: 645 SCANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSG 704
              + L   GY   +   ++G SAGG+L+G AIN  P+LF   I                
Sbjct: 603 DACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVI---------------- 662

Query: 705 SSEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLV 762
            ++VPF+D+  T+LD ++PLT  ++EE+GNPQ    ++ + SYSPYDN++ ++ YP +LV
Sbjct: 663 -AQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA-YPHLLV 673

BLAST of Spg010295 vs. ExPASy Swiss-Prot
Match: O07834 (Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 7.0e-62
Identity = 198/741 (26.72%), Postives = 328/741 (44.26%), Query Frame = 0

Query: 44  PPAAKKVPFTHSVHGVTLQDPYHWM--SNTDDPDLADYLRRENLYAESFMADTQILQRRL 103
           P  AKK     + HG    D Y+W+     ++ ++  YL  EN Y ++ MA  + L+ +L
Sbjct: 29  PDVAKKPHVVKAPHGAERNDEYYWLRDDKRENKEMLAYLNAENAYTDAVMAPLKPLEDKL 88

Query: 104 FSEMTSRMPAKVSTPPEPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNF 163
           + E+ +R+    ++ P     W+YY     GKDYPV  RR        +      A G+F
Sbjct: 89  YDEVVARIKQDDASVPYRERGWWYYARFVTGKDYPVHARRKDGPGVDAVSIQAANAAGDF 148

Query: 164 GREEEVLIDWNEIAKQYVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITG 223
              E+VL+D N +         A  ++YN          VG   VS D+  LAY  D  G
Sbjct: 149 A-GEQVLLDVNALG--------AGKDYYN----------VGDYEVSQDNRLLAYADDTNG 208

Query: 224 SEHFMLQVKDLRTGLIISKLQEGVSETLLVC--LTQVYIIDANHSLSGLQRIHKRIPGIQ 283
              + ++ K+L TG ++          L+       ++ +D +      +R+   + G  
Sbjct: 209 RRQYTIRFKNLDTGELLPDTVTNAEPNLVWSDDGRTLFYVDKDPETLLSKRVKAHVLGTP 268

Query: 284 ------YFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDF 343
                  + E    F+  +  +  +K    S E    +  +     S     ++   E  
Sbjct: 269 ASQDALVYEEEDDSFYMGIGRSRDDKFICISVESTVSSEMRCTPAASPGVFTVLAPRERD 328

Query: 344 SIQDMDIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLE--IKTLDPWF---FPLPSNS 403
                D      V+  N  G +    +  P D+  ++  +  +   D  F   F L    
Sbjct: 329 VEYQADHLGDRWVIRTNADGATNFKIVTAPTDSTSRKDWKDWVAHRDDVFVEGFELFDGF 388

Query: 404 CNVAPGSNHDFMSSLYRVVLSSPVMPDLIVDY-DMSKRVFSIIQQEEVQVKNDVKLKTYV 463
             VA  +N   + SL RV+ +     D   DY    +  +S+      +   D    +Y 
Sbjct: 389 SVVAERAN--ALESL-RVIKA-----DGSSDYVKADESAYSMGLSANPETGTDWLRYSYT 448

Query: 464 PDELDIEEVSDAQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDG-TRIPLTILYSPMT 523
                           E  Q  +  +    +  Y  +R    + DG T+IP+T++Y    
Sbjct: 449 SMTTPATTYEINTKTGERRQLKQQPVPGYDASKYVTERVWAPARDGKTKIPVTLVYRKDV 508

Query: 524 FQKGQSPGVLQGYGAYGEILDKSWCSNRLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSG 583
            + G++P +   YG+YG  +D ++    +SLLDRG V A A +RGG  +G  +W+  G  
Sbjct: 509 ARDGKAPMLQYAYGSYGASMDPNFSITNVSLLDRGVVYALAHIRGGQEMG-RAWYDDGKL 568

Query: 584 LEKQNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFRAAILKVSF 643
             K N+  DFI   ++L   GY  K+R+ ++G SAGGLL+GA  NM P+ ++  +     
Sbjct: 569 YNKINTFTDFIDVTDYLVKEGYAAKDRVAAMGGSAGGLLMGAVSNMAPEKYKVIL----- 628

Query: 644 SHGLAYTLVSGSSEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQFKSILSYSPYDNI 703
                       + VPF+D+  T+LDPT+PLT  +Y+E+GNP+ +  +  IL+YSPYDN+
Sbjct: 629 ------------TLVPFVDVVTTMLDPTIPLTTNEYDEWGNPEEKGYYDYILTYSPYDNL 688

Query: 704 SRRSCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGL 763
             ++ YP+M V     D++V  WE AK+VA++RD          + +TNM  GH G+ G 
Sbjct: 689 QAKA-YPAMFVGTGLWDSQVQYWEPAKYVARLRDLNTG--KGPVVFRTNMEAGHGGKSGR 721

Query: 764 YGGCEETAYEYAFLIKVLGTS 768
           +    E A  +AF++  LG +
Sbjct: 749 FRQYRERAEMFAFMLDQLGVA 721

BLAST of Spg010295 vs. ExPASy Swiss-Prot
Match: Q32N48 (Prolyl endopeptidase-like OS=Xenopus laevis OX=8355 GN=prepl PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.6e-42
Identity = 135/477 (28.30%), Postives = 213/477 (44.65%), Query Frame = 0

Query: 254 TQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARC 313
           ++V +ID          + KRI G+ Y++EH +G  Y+     L ++G+ +  EY + + 
Sbjct: 252 SEVRLIDNRCPFEPPVLVQKRIAGVIYYIEHSNGCLYM-----LRRHGEAA--EYKILKA 311

Query: 314 QVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLE 373
            V       W+ +    E   + DM++   H +LF+ N     L  I LP  A  +    
Sbjct: 312 AVSS-GMKHWEPVYEVQERTKLVDMEMLKDHCLLFLKNHNQLSLEVIGLPSGAVLQS--- 371

Query: 374 IKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQE 433
                     LP+ +C +      ++ +      LSSPV P +  +Y + K+  S+    
Sbjct: 372 --------IKLPAWACALELDHQAEYGAGTVGFSLSSPVHPPVHFEYSLRKKQLSV---- 431

Query: 434 EVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDG 493
                             D    SD  ++                      R E  S DG
Sbjct: 432 ------------------DTNHSSDGIHQFHTL------------------RLEAKSKDG 491

Query: 494 TRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLSLLDRGFVLAFADVRGGG 553
           T +PLT+LY     Q  Q P ++  YGAYG  L+ S+   +  L++ G++LA+  VRGGG
Sbjct: 492 TSVPLTLLYKDSEKQMRQRPLLIHVYGAYGMDLNMSFKVEKRMLVEEGWLLAYCHVRGGG 551

Query: 554 GVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMH 613
            +G  +WH  G   +K N + D  SC + L   GY   +       SAGG+L GA  N  
Sbjct: 552 ELG-CNWHSEGVLDKKLNGLEDLGSCISHLHGLGYSQPHYSAVEAASAGGVLAGALCNSA 611

Query: 614 PDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQ 673
           P LFRA +L                 E PFLD+ NT+++ +LPLTI + EE+GNP    +
Sbjct: 612 PRLFRAVVL-----------------EAPFLDVLNTMMNVSLPLTIEEQEEWGNPLSDEK 650

Query: 674 F-KSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTS 730
           + + I SY PY NI+ ++ YP + +TA  +D RV +     ++ ++R     +C  S
Sbjct: 672 YHRYIKSYCPYQNITPQN-YPCVRITAYENDQRVPIQGLLGYITRLRKAARDYCHES 650

BLAST of Spg010295 vs. ExPASy Swiss-Prot
Match: P55656 (Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a01580 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 6.8e-41
Identity = 184/814 (22.60%), Postives = 312/814 (38.33%), Query Frame = 0

Query: 41  SESPPAAKKVPFTHSVHGVTLQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRR 100
           S  PP  +       +H     D Y W+ + +DPD+  YL  EN YA+   +    L+  
Sbjct: 5   SLQPPLPRSERRIRVLHNDVTIDSYGWLRDREDPDVLAYLEAENHYADEVTSYVAELKAD 64

Query: 101 LFSEMTSRMPAKVSTPPEPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGN 160
           L +E+  R     + PP   G +FY+Q    G           +    W ++ V      
Sbjct: 65  LIAEIEKRDSCDGAPPPFQVGFFFYFQKSQSG-----------LLHSAWWRRPVT----- 124

Query: 161 FGREEEVLIDWNEIAKQYVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDIT 220
            G  EE++ D N +    V        FY+          +G    S D  ++A++ D+ 
Sbjct: 125 -GGPEELVFDPNTLPGAEV--------FYS----------LGALEPSDDGRYIAFSFDLI 184

Query: 221 GSEHFMLQVKDLRTG--------------------------------------------- 280
           G+E + L+V+D+  G                                             
Sbjct: 185 GNERYELRVRDMTNGREIWRDPSRAGRLVWAADNRTLFFTRERADRRQHDRVVRLDVETG 244

Query: 281 --------------LIISKLQEGVSETLLVCLT------------QVYIIDANHSLSGLQ 340
                         L++ +   G    + V +T            +V+ + A       +
Sbjct: 245 RSEVVFEEVNERLALVVRRSGSGAYLFIDVIITSDMSSRIQRAAAEVWCLPAERPTDMWR 304

Query: 341 RIHKRIPGIQYFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQ 400
           RI  R  G + + EH    F    N         +     + R  ++D   + WQ+++  
Sbjct: 305 RILARELGHEIYAEHWGNEFLFRVND--------TGPNLRLVRTAIDDTSPSRWQEVVPH 364

Query: 401 SEDFSIQDMDIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSC 460
               +++++ +   H+++            I   L A+++       + P   P+  +SC
Sbjct: 365 RAGITLEEIHVLEEHVIVLERE-------GIQPRLVAHHRN----GRVGPSIVPV-EHSC 424

Query: 461 NVAPG---------SNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQEEVQVKND 520
            V  G         + H +  S     + S V PD+ + +D+            +  K+ 
Sbjct: 425 TVTVGLSAGGSYSCARHPYRVSALTYKICSFVTPDIFIQHDL------------LTDKSK 484

Query: 521 VKLKTYVPD-ELDIEEVSDAQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDGTRIPLT 580
           V  +T V   E ++ E      K E                           DG  +P++
Sbjct: 485 VLYRTLVSGFEPELYEARVVMAKAE---------------------------DGVEVPIS 544

Query: 581 ILYSPMTFQKGQSPGVLQGYGAYG-EILDK--SWCSN---RLSLLDRGFVLAFADVRGGG 640
           I+      + G  P +L  YG YG + L     W S+   RLSLLDRG       VRGGG
Sbjct: 545 IVARRDRGEDG--PVLLNVYGCYGAQSLPAFFGWPSSMTARLSLLDRGVAFGIVHVRGGG 604

Query: 641 GVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMH 700
            +G  +WH   +  +K+ +  D I+ A  L ++ +  ++ +   G SAGG  V AA  + 
Sbjct: 605 ELG-RAWHEAATRDQKRLTHTDLIAAAECLVEHRFASRDGIVIEGRSAGGGTVLAAAVLR 664

Query: 701 PDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQ 760
           PDLFRA +                 +EVP  DI +T LD TLP  + +  E+G+P +   
Sbjct: 665 PDLFRAVL-----------------AEVPLADIIDTELDFTLPYALRETAEYGDPHLAND 703

Query: 761 FKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILK 768
           ++ + SY PY N++    YP   + A+ HD++V  ++ A++VA+ R           I +
Sbjct: 725 YQYLRSYDPYYNLTPDRRYPPTYIDAALHDSQVLYYQPARYVAQRRSKAVDR-DPDLIFR 703

BLAST of Spg010295 vs. ExPASy TrEMBL
Match: A0A6J1ILQ3 (Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111477597 PE=3 SV=1)

HSP 1 Score: 1265.4 bits (3273), Expect = 0.0e+00
Identity = 636/834 (76.26%), Postives = 684/834 (82.01%), Query Frame = 0

Query: 1   MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVHGVT 60
           MALKSLLKPKC  RK  LSSS  S S FSSLCKERIFSLPSESPP AKKVPFTHSVHG+T
Sbjct: 1   MALKSLLKPKCFTRKAILSSSLASSSLFSSLCKERIFSLPSESPPTAKKVPFTHSVHGIT 60

Query: 61  LQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPEPW 120
           LQDPYHWM+NT DPDLADYLRRENLYAE+FMADTQILQRRLFSEMTSR+P KVSTPPEPW
Sbjct: 61  LQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRIPTKVSTPPEPW 120

Query: 121 GPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQYVM 180
           GPWFYYQYIPEGK+YPVLCRRL  +K  WLKK  QFAKGN G++EEVL+DWNEIAKQY  
Sbjct: 121 GPWFYYQYIPEGKEYPVLCRRLLNQKTNWLKKLTQFAKGNSGKQEEVLLDWNEIAKQY-- 180

Query: 181 KIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLIISK 240
                           GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDLR+GL+I K
Sbjct: 181 ----------------GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPK 240

Query: 241 LQEGV------------------------------------SETLLV--------CL--- 300
           LQEGV                                     E +LV        C+   
Sbjct: 241 LQEGVVSLAWAEEGRTLFYTQADENQRPYRVFSTKLGFSNTEEDVLVFVENDPNYCVDIT 300

Query: 301 -----------------TQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTNAP 360
                            ++VYIIDAN+SLSGLQRIHKRIPGIQYFLEHH GFFYILTNAP
Sbjct: 301 STKDGKFITVNSNSRTSSEVYIIDANNSLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAP 360

Query: 361 LEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGVSM 420
           LEK GDC KE+YYVARC+VEDIKSA+WQDI+LQS+DFSIQDMD+F+GHLVLFVN  GV M
Sbjct: 361 LEKKGDCLKEDYYVARCRVEDIKSANWQDIVLQSQDFSIQDMDVFSGHLVLFVNKNGVPM 420

Query: 421 LCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDL 480
           LCSINLPLDAN+K  LEI+ LDPWFFPLPSNSC+V+PGSNHDFMSSLYRVVLSSP+MPDL
Sbjct: 421 LCSINLPLDANHKHCLEIEKLDPWFFPLPSNSCSVSPGSNHDFMSSLYRVVLSSPLMPDL 480

Query: 481 IVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKD 540
           IVDYDMSKRVFSIIQQEEV+VK+DVKLKTY P+ L IE+VSDAQNKRENF+N ES+ WKD
Sbjct: 481 IVDYDMSKRVFSIIQQEEVEVKHDVKLKTYQPNALGIEKVSDAQNKRENFENRESKTWKD 540

Query: 541 FSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLS 600
           FSD+YCC+RKEVISHDG R+PLTILYSP TFQKG+S GVLQGYGAYGE+LDKSWC +RLS
Sbjct: 541 FSDSYCCERKEVISHDGIRVPLTILYSPSTFQKGRSLGVLQGYGAYGEVLDKSWCPSRLS 600

Query: 601 LLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGS 660
           LLDRGFVLAFAD+RGGGG GDSSWHR GSGLEKQNSI DFI CANFL DNGYVHKNRL S
Sbjct: 601 LLDRGFVLAFADIRGGGG-GDSSWHRSGSGLEKQNSIQDFIFCANFLIDNGYVHKNRLAS 660

Query: 661 IGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLP 720
           IGYSAGGLLVGAAINMHPDLFRAAILK                 VPFLDICNTLLDP+LP
Sbjct: 661 IGYSAGGLLVGAAINMHPDLFRAAILK-----------------VPFLDICNTLLDPSLP 720

Query: 721 LTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVA 771
           LTILDYEEFGNPQI +QF+SILSYSPYDNIS+ SCYP MLVTASF DARVGVWEAAKWVA
Sbjct: 721 LTILDYEEFGNPQIAMQFESILSYSPYDNISKGSCYPPMLVTASFRDARVGVWEAAKWVA 780

BLAST of Spg010295 vs. ExPASy TrEMBL
Match: A0A6J1GZW2 (Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111458759 PE=3 SV=1)

HSP 1 Score: 1258.8 bits (3256), Expect = 0.0e+00
Identity = 634/834 (76.02%), Postives = 681/834 (81.65%), Query Frame = 0

Query: 1   MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVHGVT 60
           MALKSLLKP    R   L SS  S S FSSLCKERIFSLPSESPPAAKKVPFTHSVHG+T
Sbjct: 1   MALKSLLKP---TRNSILCSSISSSSLFSSLCKERIFSLPSESPPAAKKVPFTHSVHGIT 60

Query: 61  LQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPEPW 120
           LQDPYHWM+NT DPDLADYLRRENLYAE+FMADTQILQRRLFSEMTSR+  KVSTPPEPW
Sbjct: 61  LQDPYHWMANTADPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRISTKVSTPPEPW 120

Query: 121 GPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQYVM 180
           GPWFYYQYIPEGK+YPVLCRRLQ EK  WLKK  QFAKGN G++EEVL+DWNEIAKQY  
Sbjct: 121 GPWFYYQYIPEGKEYPVLCRRLQNEKTNWLKKLTQFAKGNSGKQEEVLLDWNEIAKQY-- 180

Query: 181 KIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLIISK 240
                           GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQ+KDLR+GL+I K
Sbjct: 181 ----------------GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQIKDLRSGLMIPK 240

Query: 241 LQEGV------------------------------------SETLLV--------CL--- 300
           LQEGV                                     E +LV        C+   
Sbjct: 241 LQEGVVSLAWAEEGRTLFYTQADENQRPYRVFSTKLGFSDTGEDVLVFVENDPNYCVDIT 300

Query: 301 -----------------TQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTNAP 360
                            ++VYIIDAN+ LSGLQRIHKRIPGIQYFLEHH GFFYILTNAP
Sbjct: 301 STKDGKFITVNSNSRTSSEVYIIDANNWLSGLQRIHKRIPGIQYFLEHHCGFFYILTNAP 360

Query: 361 LEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGVSM 420
           LEK GDCSKE+YYVARC+VEDIKSA+WQDI+LQS+DFSI DMD+F+GHLVLFVN  GV M
Sbjct: 361 LEKKGDCSKEDYYVARCRVEDIKSANWQDIVLQSKDFSIHDMDVFSGHLVLFVNKNGVPM 420

Query: 421 LCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDL 480
           LCSINLPLDAN+K RLEI+ LDPWFFPLPSNSC+VAPGSNHDF SSLYRVVLSSPVMPDL
Sbjct: 421 LCSINLPLDANHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFTSSLYRVVLSSPVMPDL 480

Query: 481 IVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKD 540
           IVDYDMSKRVFSIIQQEEV+VK+D+KLKTY PD L IE+VSDAQNKRENF+  ES+ WKD
Sbjct: 481 IVDYDMSKRVFSIIQQEEVEVKHDIKLKTYQPDALGIEKVSDAQNKRENFETRESETWKD 540

Query: 541 FSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLS 600
           FSD+YCC+RKEVISHDG R+PLTILYSP TFQKG+SPGVLQGYGAYGE+LDKSWC +RLS
Sbjct: 541 FSDSYCCERKEVISHDGIRVPLTILYSPSTFQKGRSPGVLQGYGAYGEVLDKSWCPSRLS 600

Query: 601 LLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGS 660
           LLDRGFVLAFAD+RGGGG GDSSWHRCGSGL+KQNSI DFI CANFL DNGYVHKNRLGS
Sbjct: 601 LLDRGFVLAFADIRGGGG-GDSSWHRCGSGLQKQNSIQDFIFCANFLIDNGYVHKNRLGS 660

Query: 661 IGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLP 720
           IGYSAGGLLVGAAINMHPDLF AAILK                 VPFLDICNTLLDP+LP
Sbjct: 661 IGYSAGGLLVGAAINMHPDLFGAAILK-----------------VPFLDICNTLLDPSLP 720

Query: 721 LTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVA 771
           LTILDYEEFGNP+I +QF+SILSYSPYDNIS+ SCYP MLVTASF DARVGVWEAAKWVA
Sbjct: 721 LTILDYEEFGNPEIAMQFESILSYSPYDNISKGSCYPPMLVTASFRDARVGVWEAAKWVA 780

BLAST of Spg010295 vs. ExPASy TrEMBL
Match: A0A6J1D5U2 (Prolyl endopeptidase OS=Momordica charantia OX=3673 GN=LOC111017556 PE=3 SV=1)

HSP 1 Score: 1258.0 bits (3254), Expect = 0.0e+00
Identity = 644/835 (77.13%), Postives = 682/835 (81.68%), Query Frame = 0

Query: 1   MALKSLL--KPKCSIRKFFL-SSSSISVSRFSSLCKERIFSLPSESPPAAKKVPFTHSVH 60
           MALKSLL  KPKCSI+K  L SSSS S S FSS C++R FSLPSESPPAAKKVPF +SVH
Sbjct: 17  MALKSLLRVKPKCSIKKLLLPSSSSPSSSLFSSFCRDRSFSLPSESPPAAKKVPFKYSVH 76

Query: 61  GVTLQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           GVTLQDP+HWMSNTDDPDLADYLRRENLYAE+FMADTQILQRRLFSEMTSRMPAKVSTPP
Sbjct: 77  GVTLQDPFHWMSNTDDPDLADYLRRENLYAEAFMADTQILQRRLFSEMTSRMPAKVSTPP 136

Query: 121 EPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGR-EEEVLIDWNEIAK 180
           EPWGPWFYYQYIP GK+YPVLCRRLQ EK GWLKK VQFA+GNFG+ EEEVL+DWNEIAK
Sbjct: 137 EPWGPWFYYQYIPAGKEYPVLCRRLQNEKIGWLKKLVQFARGNFGKEEEEVLLDWNEIAK 196

Query: 181 QYVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGL 240
            Y                  GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDL +GL
Sbjct: 197 HY------------------GYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLGSGL 256

Query: 241 IISKLQEGV--------SETLL-----------------------------------VCL 300
           II K Q+GV          TL                                     C+
Sbjct: 257 IIPKSQKGVVSLAWAEEGRTLFYTQSDENQRPYRVFCTKVGCSDAEDVSVFVENDPNFCV 316

Query: 301 --------------------TQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILT 360
                               ++VYIIDAN+ LSGLQRIHKRIPGIQYFLEHH GFFYILT
Sbjct: 317 DVTSTKDGKFITVNSNSRTSSEVYIIDANNPLSGLQRIHKRIPGIQYFLEHHFGFFYILT 376

Query: 361 NAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKG 420
           NAPLEKNGDCSKEEYYVARC+VEDIKS+DWQD ILQSEDFSIQDMDIF+GHLVLFVN  G
Sbjct: 377 NAPLEKNGDCSKEEYYVARCRVEDIKSSDWQDAILQSEDFSIQDMDIFSGHLVLFVNKMG 436

Query: 421 VSMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVM 480
           VSMLC+INLPLD N+K RLEI+ LDPWFFPLPSNSC+VAPGSNHDFMSSLYRVVLSSPVM
Sbjct: 437 VSMLCAINLPLDTNHKHRLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVM 496

Query: 481 PDLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQI 540
           PDL+VDYDMSKRVFSIIQQEEVQVK+DV+LKT +PDELD+EEVS A+NK  NFQNSESQI
Sbjct: 497 PDLVVDYDMSKRVFSIIQQEEVQVKHDVQLKTCLPDELDVEEVSTAENKIANFQNSESQI 556

Query: 541 WKDFSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSN 600
            KDFSDAYCC+RKEVISHDG RIPLTILYSP+ F KG+SPGVL GYGAYGEILDKSWC  
Sbjct: 557 SKDFSDAYCCERKEVISHDGIRIPLTILYSPVNFHKGRSPGVLHGYGAYGEILDKSWCPY 616

Query: 601 RLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNR 660
           RLSLLDRGFVLAFADVRGGGG GDSSWHR GSGLEKQNSIHDFISCA FL DN YVHKN+
Sbjct: 617 RLSLLDRGFVLAFADVRGGGGGGDSSWHRSGSGLEKQNSIHDFISCAKFLVDNDYVHKNQ 676

Query: 661 LGSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDP 720
           LGSIGYSAGGLLVGAAINM PDLFRAAILK                 VPFLDICNTLLDP
Sbjct: 677 LGSIGYSAGGLLVGAAINMRPDLFRAAILK-----------------VPFLDICNTLLDP 736

Query: 721 TLPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAK 769
           +LPLTILDYEEFGNPQ+  QF+SIL+YSPYDNISR SCYP MLVT+SF DARVGVWEAAK
Sbjct: 737 SLPLTILDYEEFGNPQLPKQFESILNYSPYDNISRGSCYPPMLVTSSFRDARVGVWEAAK 796

BLAST of Spg010295 vs. ExPASy TrEMBL
Match: A0A0A0KQZ9 (Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G606880 PE=3 SV=1)

HSP 1 Score: 1232.6 bits (3188), Expect = 0.0e+00
Identity = 620/827 (74.97%), Postives = 672/827 (81.26%), Query Frame = 0

Query: 1   MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKER---IFSLPSESPPAAKKVPFTHSVH 60
           MALK+LLKPK SI KFFLSS S     FSS CK++   IFS PS+SPP+ KK+PFTHSVH
Sbjct: 1   MALKTLLKPKSSITKFFLSSLS-----FSSFCKQQQQPIFSFPSQSPPSPKKLPFTHSVH 60

Query: 61  GVTLQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPP 120
           GV LQDPYHWMSNT DPD ADYLR+ENLYAE+FMADTQ+LQR+LFSEMTSR+PAKVSTPP
Sbjct: 61  GVKLQDPYHWMSNTHDPDFADYLRQENLYAEAFMADTQVLQRQLFSEMTSRIPAKVSTPP 120

Query: 121 EPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQ 180
           EPWGPWFYYQYIP+GK+YPVLCRRLQ EK  W +K + F KGN G+EE+VL+DWNEIAKQ
Sbjct: 121 EPWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFRKILNFGKGNSGKEEQVLLDWNEIAKQ 180

Query: 181 YVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLI 240
           Y                  GYVHVGTCR+SPDHNFLAYTVDITG+EHFMLQ+KDLR GLI
Sbjct: 181 Y------------------GYVHVGTCRISPDHNFLAYTVDITGNEHFMLQIKDLRNGLI 240

Query: 241 ISKLQ-EGV------SETLLVCLTQ----------------------------------- 300
           I KLQ EGV       E  ++  TQ                                   
Sbjct: 241 IPKLQKEGVVSLAWAEEGRVLFYTQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCV 300

Query: 301 ------------VYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTNAPLEKNGDC 360
                       VYIIDAN+SL GLQRIH+RIPGIQYFLEHH GFFYILTNAPLEKNG C
Sbjct: 301 DITSTKDGKFITVYIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTNAPLEKNGGC 360

Query: 361 SKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGVSMLCSINLP 420
           S+E+YYVARC+VEDIKSADWQDI+LQSEDFSIQDMDIF+GHLVLFVN  GVSMLCSIN P
Sbjct: 361 SEEDYYVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGVSMLCSINFP 420

Query: 421 LDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMS 480
           LDA++   LEI  LDPWFFPLPSNSC+VAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMS
Sbjct: 421 LDADHNHHLEIDKLDPWFFPLPSNSCSVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMS 480

Query: 481 KRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKDFSDAYCC 540
           KR+FSIIQQEEV+V++DV+LKT +PD LD EEVSD Q+KRENFQN ESQ WKDFS+AY C
Sbjct: 481 KRIFSIIQQEEVKVQHDVELKTNLPDTLDAEEVSDTQSKRENFQNCESQNWKDFSEAYFC 540

Query: 541 DRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLSLLDRGFV 600
           +R EV SHDG RIPLTILYSPMTF+KGQSPG+LQGYGAYGEILDKSWC  RLSLLDRGFV
Sbjct: 541 ERIEVKSHDGIRIPLTILYSPMTFKKGQSPGILQGYGAYGEILDKSWCPYRLSLLDRGFV 600

Query: 601 LAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGG 660
           LAFADVRGGGG GDSSWHRCGSGLEK NSIHDFISCANFL  NGYVHK+RLGSIGYSAGG
Sbjct: 601 LAFADVRGGGGGGDSSWHRCGSGLEKPNSIHDFISCANFLIHNGYVHKDRLGSIGYSAGG 660

Query: 661 LLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLPLTILDYE 720
           LLVGAAINMHP+LFRAAILK                 VPFLDICNTLLDP+LPLT+LDYE
Sbjct: 661 LLVGAAINMHPNLFRAAILK-----------------VPFLDICNTLLDPSLPLTVLDYE 720

Query: 721 EFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTC 771
           EFGNPQI+ QF+SILSYSPYDNIS+ +CYPSMLVTASFHDARVGVWEAAKWVAKIRDTTC
Sbjct: 721 EFGNPQIQKQFESILSYSPYDNISKGTCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTC 780

BLAST of Spg010295 vs. ExPASy TrEMBL
Match: A0A1S3BF53 (Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103489202 PE=3 SV=1)

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 616/836 (73.68%), Postives = 672/836 (80.38%), Query Frame = 0

Query: 1   MALKSLLKPKCSIRKFFLSSSSISVSRFSSLCKER--IFSLPSESPPAAKKVPFTHSVHG 60
           MALKSLLKPK SI KFFLSS S     FSS CK++  IFS P +SPP+ KK+PFTHSVHG
Sbjct: 13  MALKSLLKPKSSITKFFLSSLS-----FSSFCKQQQPIFSFPPQSPPSPKKLPFTHSVHG 72

Query: 61  VTLQDPYHWMSNTDDPDLADYLRRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPE 120
           VTLQDPYHWMSNT DPDL+DYLR+ENLYAE+FMADT++LQR+LFSEMT R+P+KVSTPPE
Sbjct: 73  VTLQDPYHWMSNTHDPDLSDYLRQENLYAEAFMADTRVLQRQLFSEMTGRIPSKVSTPPE 132

Query: 121 PWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQY 180
           PWGPWFYYQYIP+GK+YPVLCRRLQ EK  W KK +QF KGNFG+EE+VL+DWNEIAK+Y
Sbjct: 133 PWGPWFYYQYIPDGKEYPVLCRRLQNEKSSWFKKILQFGKGNFGKEEQVLLDWNEIAKRY 192

Query: 181 VMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVDITGSEHFMLQVKDLRTGLII 240
                             GYVHVGTCRVSPDHNFLAYTVDITG EHFMLQ+KDLR GLII
Sbjct: 193 ------------------GYVHVGTCRVSPDHNFLAYTVDITGDEHFMLQIKDLRNGLII 252

Query: 241 SKLQ-EGV------SETLLVCLTQ------------------------------------ 300
            KLQ EGV       E  ++  TQ                                    
Sbjct: 253 PKLQKEGVVSLAWAEEGRMLFYTQADENQRPYRVFCTKVGFNDTEDVSVFVENDPNYCVD 312

Query: 301 ---------------------VYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGFFYILTN 360
                                VYIIDAN+SL GLQRIH+RIPGIQYFLEHH GFFYILTN
Sbjct: 313 ITSTKDGKFVTVNSNSRTSSEVYIIDANNSLGGLQRIHERIPGIQYFLEHHHGFFYILTN 372

Query: 361 APLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLFVNNKGV 420
           APLEKN DC +E+YYVARC+VEDIKSADWQDI+LQSEDFSIQDMDIF+GHLVLFVN  GV
Sbjct: 373 APLEKNVDCLEEDYYVARCRVEDIKSADWQDIVLQSEDFSIQDMDIFSGHLVLFVNKNGV 432

Query: 421 SMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVLSSPVMP 480
           SMLCSINLPLDA+    LEI+ LDPWFFPLPSNSC+VAPGSNHDFMSS YRVVLSSPVMP
Sbjct: 433 SMLCSINLPLDADDNHHLEIEKLDPWFFPLPSNSCSVAPGSNHDFMSSSYRVVLSSPVMP 492

Query: 481 DLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIW 540
           DLIVDYDMSKR FSIIQQEEV+V++DV+LKT +PD LD++EVSD QNKRENFQN +SQ W
Sbjct: 493 DLIVDYDMSKRTFSIIQQEEVKVQHDVELKTNLPDTLDVQEVSDTQNKRENFQNCDSQNW 552

Query: 541 KDFSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNR 600
           KDFS+AYCC+R EV SHDG  IPLTILY+PMTFQKGQSPGVLQGYGAYGEILDKSWC  R
Sbjct: 553 KDFSEAYCCERIEVTSHDGVGIPLTILYTPMTFQKGQSPGVLQGYGAYGEILDKSWCPYR 612

Query: 601 LSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRL 660
           LSLLDRGFVLAFADVRGGGG GDSSWHR G+GLEK NSIHDF+SCANFL +NGYVHK+RL
Sbjct: 613 LSLLDRGFVLAFADVRGGGGGGDSSWHRSGNGLEKPNSIHDFVSCANFLINNGYVHKDRL 672

Query: 661 GSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPT 720
           GSIGYSAGGLLVGAAINMHP+LFRAAILK                 VPFLDICNTLLDP+
Sbjct: 673 GSIGYSAGGLLVGAAINMHPNLFRAAILK-----------------VPFLDICNTLLDPS 732

Query: 721 LPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKW 771
           LPLT+LDYEEFGNPQI+ QF+SILSYSPY+NIS+ SCYPSMLVTASFHDARVGVWEAAKW
Sbjct: 733 LPLTVLDYEEFGNPQIQKQFESILSYSPYENISKGSCYPSMLVTASFHDARVGVWEAAKW 792

BLAST of Spg010295 vs. TAIR 10
Match: AT1G69020.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 807.4 bits (2084), Expect = 1.0e-233
Identity = 406/781 (51.98%), Postives = 529/781 (67.73%), Query Frame = 0

Query: 24  SVSRFSSLC---KERIFSLPSESPPAAKKVPFTHSVHGVTLQDPYHWMSNTDDPDLADYL 83
           SV  FS+ C   +    S+P+E+PP  KK+PF  S HG+T QDP+HWM NTDD D  D+L
Sbjct: 17  SVLSFSTKCFVGRTSSLSVPTEAPPVPKKIPFAISSHGITRQDPFHWMKNTDDTDFVDFL 76

Query: 84  RRENLYAESFMADTQILQRRLFSEMTSRMPAKVSTPPEPWGPWFYYQYIPEGKDYPVLCR 143
           +REN Y+++FMADT+ L+R LFSEM +R+P ++ TPPE WG W Y QYIP+GK+YP+LCR
Sbjct: 77  KRENSYSQAFMADTETLRRDLFSEMKTRIPEEIFTPPERWGQWLYRQYIPKGKEYPLLCR 136

Query: 144 RLQIEKGGWLKKFVQFAKGNFGREEEVLIDWNEIAKQYVMKIMAFINFYNELLMIVGYVH 203
           RL+  K  WL    +      G EEEV++DWN+IA+Q+                  GYVH
Sbjct: 137 RLEKGKTNWLSGLFR------GEEEEVVLDWNQIAEQF------------------GYVH 196

Query: 204 VGTCRVSPDHNFLAYTVDITG-----------------------------SEHFMLQVKD 263
           VG CRVSPDHN+LAYTVD  G                              +  +   +D
Sbjct: 197 VGVCRVSPDHNYLAYTVDPEGDGITLFYTVTDENQRPHRVVVTNVESDGRDDAVVFTERD 256

Query: 264 LRTGLIISKLQEGVSETL---LVCLTQVYIIDANHSLSGLQRIHKRIPGIQYFLEHHSGF 323
               + I+  ++G   T+       ++VYI++A+  ++GLQR  +R+PG+Q FLEHH+GF
Sbjct: 257 SSFCVDITTTKDGKFVTINSNSRTSSEVYIVNADKPMAGLQRTRERVPGVQCFLEHHNGF 316

Query: 324 FYILTNAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSIQDMDIFNGHLVLF 383
           FYILTN+P     + S E YY+ RC VE+I+++DWQ +    +D  IQDMD+FN +LVL+
Sbjct: 317 FYILTNSPSNAISEWSGEGYYLTRCLVEEIEASDWQTVFRPDDDVVIQDMDMFNDYLVLY 376

Query: 384 VNNKGVSMLCSINLPLDANYKQRLEIKTLDPWFFPLPSNSCNVAPGSNHDFMSSLYRVVL 443
           +N KG+ MLCSI++P+ AN K    +  L PW+FPLP +SC+VAPGSNHDF SS+YRVVL
Sbjct: 377 LNKKGLPMLCSIDMPIKANTK---HMDDLVPWYFPLPVDSCSVAPGSNHDFQSSIYRVVL 436

Query: 444 SSPVMPDLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDELDIE---EVSDAQNKREN 503
           SSPV+PD IVDYD+S+R+FSI+QQE   V N    K +   +   E   +++D  ++ E+
Sbjct: 437 SSPVIPDTIVDYDVSRRLFSIVQQEGGVVDNSDSSKPWYTADRSTENNGQLNDRTSEGED 496

Query: 504 FQ-NSESQIWKDFSDAYCCDRKEVISHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGE 563
            Q +S    W+D SD Y C+R+EV SHDG  +PLTILYS   ++K +SPG+L GYGAYGE
Sbjct: 497 GQLDSRMPKWEDLSDTYVCERQEVSSHDGVEVPLTILYSREAWKKSESPGMLIGYGAYGE 556

Query: 564 ILDKSWCSNRLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLT 623
           +LDKSWC+NRLS+LDRG+V+AFADVRGGG  G+ SWH+ G+   KQNSI DFI  A +L 
Sbjct: 557 VLDKSWCTNRLSMLDRGWVIAFADVRGGGS-GEFSWHKSGTRSLKQNSIQDFIYSAKYLV 616

Query: 624 DNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGLAYTLVSGSSEVPFL 683
           + GYVH++ L ++GYSAG +L  AA+NMHP LF+A ILK                 VPF+
Sbjct: 617 EKGYVHRHHLAAVGYSAGAILPAAAMNMHPSLFQAVILK-----------------VPFV 676

Query: 684 DICNTLLDPTLPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRRSCYPSMLVTASFHDA 743
           D+ NTL DP LPLT+LD+EEFGNP  +  F SILSYSPYD I +  CYPSMLVT SFHD+
Sbjct: 677 DVLNTLSDPNLPLTLLDHEEFGNPDNQTDFGSILSYSPYDKIRKDVCYPSMLVTTSFHDS 736

Query: 744 RVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVL 766
           RVGVWE AKWVAKIRD+TC  CS + ILKTNM GGHFGEGG Y  CEETA++YAFL+KV+
Sbjct: 737 RVGVWEGAKWVAKIRDSTCHDCSRAVILKTNMNGGHFGEGGRYAQCEETAFDYAFLLKVM 752

BLAST of Spg010295 vs. TAIR 10
Match: AT5G66960.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 446.4 bits (1147), Expect = 4.5e-125
Identity = 281/805 (34.91%), Postives = 401/805 (49.81%), Query Frame = 0

Query: 40  PSESPPAAKKVPFTHSVHGVTLQDPYHWMSNTDDP----DLADYLRRENLYAESFMADTQ 99
           P  + P   K P + + H  T +DPY WMS  +D      +  Y+ +E  Y E+ +ADT 
Sbjct: 35  PPPALPKPPKKPQSFTFHDATWEDPYSWMSKLEDKVAMRHMDIYMEQEEKYTEAVLADTD 94

Query: 100 ILQRRLFSEMTSRMPAKVSTPPEPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQ 159
            +Q +L SEM SR+  ++STPP  WGPW YY+ + EGK YPVLCRRL        ++F+ 
Sbjct: 95  RIQTKLQSEMASRLSFELSTPPLRWGPWLYYRRVEEGKQYPVLCRRL----ASLHEEFIS 154

Query: 160 FAKGNFG-------REEEVLIDWNEIAKQYVMKIMAFINFYNELLMIVGYVHVGTCRVSP 219
                 G       R E+ L+D+N+ A+++                  GY +     +SP
Sbjct: 155 HKSPAAGFDYTSGKRIEQKLLDYNQEAERF-----------------GGYAYEEMSEISP 214

Query: 220 DHNFLAYTVDITGSEHFMLQVKDLRTGLIISKLQ-------------------------- 279
           DH FLAYT+    +++F L V++L +G + SK                            
Sbjct: 215 DHKFLAYTMYDKDNDYFKLCVRNLNSGALCSKPHADRVSNIAWAKNGQALLYVVTDQKKR 274

Query: 280 ----------------------EG---------------VSETLLVCLTQVYIIDANHSL 339
                                 EG                  T     ++V++I+A    
Sbjct: 275 PCRIYCSTIGSTDEDVLLHEEFEGNVHVNIRHTKDFHFVTVNTFSTTFSKVFLINAADPF 334

Query: 340 SGLQRIHKRIPGIQYFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARCQVE-DIKSADWQ 399
           SGL  + +        +EHH GF Y+ TNA    N   + + +Y+ R  V        W+
Sbjct: 335 SGLALVWEHNAPAHCIIEHHQGFLYLFTNA---SNDGGTVDHHYLLRSPVHFSSCQRIWE 394

Query: 400 DIILQSEDFSIQDMDIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLEIKTLDPWFFPL 459
            + +   +  I+D+D    HL L V       +C ++LPL    +  + ++ + P + PL
Sbjct: 395 TVFINDPELIIEDVDFCKKHLSLIVKEMQSFKICVVDLPLKTK-RVPVHLRDIKPRYLPL 454

Query: 460 PSNSCNVAPGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQ-----EEVQVKN 519
           P +   + PG+N+DF S   R  +SS VMPD +VDYD+    ++I+QQ     E  +V  
Sbjct: 455 PKHVSQIFPGTNYDFNSPTMRFTISSLVMPDAVVDYDLLNGKWNIVQQQNMLHERTRVLY 514

Query: 520 DVKLKTYVPDELDIEEVSDAQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDGTRIPLT 579
                T  P+            +    +N    +W D ++ Y CD  EV SHDG  +PL+
Sbjct: 515 GTANSTESPNIPSGTRTVSFDTEDTTAEN--DNLWNDLTEFYACDYHEVSSHDGAMVPLS 574

Query: 580 ILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLSLLDRGFVLAFADVRGGGGVGDSS 639
           I+YS    ++ Q PG+L  +GAYGE+LDK W S   SLLDRG+VLA+ADVRGGGG G   
Sbjct: 575 IVYSRAQKEENQKPGLLHVHGAYGEMLDKRWRSELKSLLDRGWVLAYADVRGGGGKG-KK 634

Query: 640 WHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFRA 699
           WH+ G G +K NSI D+I CA +L +N  V +N+L   GYSAGGL+V +AIN  PDLF+A
Sbjct: 635 WHQDGRGAKKLNSIKDYIQCAKYLVENNIVEENKLAGWGYSAGGLVVASAINHCPDLFQA 694

Query: 700 AILKVSFSHGLAYTLVSGSSEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQFKSILS 759
           A+LK                 VPFLD  +TL+ P LPLT  DYEEFG P     F +I  
Sbjct: 695 AVLK-----------------VPFLDPTHTLIYPILPLTAEDYEEFGYPGDINDFHAIRE 754

Query: 760 YSPYDNISRRSCYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGG 765
           YSPYDNI +   YP++LVT+SF + R GVWEAAKWVA++RD T +      +L    L  
Sbjct: 755 YSPYDNIPKDVLYPAVLVTSSF-NTRFGVWEAAKWVARVRDNTFNDPERPVLLN---LTT 790

BLAST of Spg010295 vs. TAIR 10
Match: AT1G50380.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 295.8 bits (756), Expect = 9.9e-80
Identity = 222/794 (27.96%), Postives = 347/794 (43.70%), Query Frame = 0

Query: 41  SESPPAAKKVPFTHSVHGVTLQDPYHWM--SNTDDPDLADYLRRENLYAESFMADTQILQ 100
           S SPP AKKV     + G    D Y+W+   +  +PD+  YLR EN Y +  M+ T+  +
Sbjct: 4   SRSPPVAKKVEHVMEMFGDVRVDNYYWLRDDSRTNPDMLSYLREENHYTDFVMSGTKQFE 63

Query: 101 RRLFSEMTSRMPAKVSTPPEPWGPWFYYQYIPEGKDYPVLCRRLQIEKGGWLKKFVQFAK 160
            +LF+E+  R+     + P   GP++YY+   +GK+Y   CRRL  +       +     
Sbjct: 64  NQLFAEIRGRIKEDDISAPLRKGPYYYYEKNLQGKEYIQHCRRLITDNKAEPSVYDTMPT 123

Query: 161 GNFGREEEVLIDWNEIAKQYVMKIMAFINFYNELLMIVGYVHVGTCRVSPDHNFLAYTVD 220
           G     E V++D N  A+++                   Y  +G  + SPDH  +AY  D
Sbjct: 124 GPDAPPEHVILDENTKAQEH------------------DYYRIGAFKASPDHKLVAYAED 183

Query: 221 ITGSEHFMLQVKDLRT---------------------GLIISKLQE------------GV 280
             G E + + V D                         L+   + E            G 
Sbjct: 184 TKGDEIYTVNVIDSEALKPVGQQLKGLTSYLEWAGNDALLYITMDEILRPDKVWLHKLGT 243

Query: 281 SETLLVCLTQ------------------------------VYIIDANHSLSGLQRIHKRI 340
            ++  VCL                                V+ +D + +  GL+ +  R+
Sbjct: 244 EQSSDVCLYHEKDDMFSLELHASESHKYLFVASESKTTRFVFSLDVSKTQDGLRVLTPRV 303

Query: 341 PGIQYFLEHHSGFFYILTNAPLEKNGDCSKEEYYVARCQVEDIKSADWQDIILQSEDFSI 400
            GI   + H    F+I   +    N +       +  C V+D  ++    ++   E   I
Sbjct: 304 DGIDSSVSHRGNHFFIQRRSTEFYNSE-------LIACPVDD--TSKTTVLLPHRESVKI 363

Query: 401 QDMDIFNGHLVLFVNNKGVSMLCSINLPLDANYKQRLE----IKTLDPWFFPLPSNSCNV 460
           Q++ +F  HL +F    G+  +    LP +    + L+    +  +DP +          
Sbjct: 364 QEIQLFRDHLAVFERENGLQKITVHRLPAEGQPLEGLQGGRNVSFVDPVY---------S 423

Query: 461 APGSNHDFMSSLYRVVLSSPVMPDLIVDYDMSKRVFSIIQQEEVQVKNDVKLKTYVPDEL 520
              +  +F S + R    S   P  + DYDM            V  K D  L  +     
Sbjct: 424 VDSTESEFSSRVLRFKYCSMKTPPSVYDYDMDSGT-------SVVKKIDTVLGGF----- 483

Query: 521 DIEEVSDAQNKRENFQNSESQIWKDFSDAYCCDRKEVISHDGTRIPLTILYS-PMTFQKG 580
                 DA N                   Y  +RK V + DGT+IP++I+Y+  +    G
Sbjct: 484 ------DASN-------------------YVTERKWVAASDGTQIPMSIVYNKKLAKLDG 543

Query: 581 QSPGVLQGYGAYGEILDKSWCSNRLSLLDRGFVLAFADVRGGGGVGDSSWHRCGSGLEKQ 640
             P +L GYG+Y   +D  + ++RLSLLDRGF    A VRGGG +G   W+  G  L+K+
Sbjct: 544 SDPLLLYGYGSYEISVDPYFKASRLSLLDRGFTFVIAHVRGGGEMG-RQWYENGKLLKKK 603

Query: 641 NSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGAAINMHPDLFRAAILKVSFSHGL 700
           N+  DFI+CA  L +  Y  K +L   G SAGGLL+GA +NM PDLF+  I         
Sbjct: 604 NTFTDFIACAERLIELKYCSKEKLCMEGRSAGGLLMGAVVNMRPDLFKVVI--------- 663

Query: 701 AYTLVSGSSEVPFLDICNTLLDPTLPLTILDYEEFGNPQIRVQFKSILSYSPYDNISRRS 760
                   + VPF+D+  T+LDPT+PLT  ++EE+G+P+    +  + SYSP DN++ ++
Sbjct: 664 --------AGVPFVDVLTTMLDPTIPLTTSEWEEWGDPRKEEFYFYMKSYSPVDNVTAQN 703

Query: 761 CYPSMLVTASFHDARVGVWEAAKWVAKIRDTTCSHCSTSAILKTNMLGGHFGEGGLYGGC 765
            YP+MLVTA  +D RV   E  KWVAK+R+      +   + K  +  GHF + G +   
Sbjct: 724 -YPNMLVTAGLNDPRVMYSEPGKWVAKLREMKTD--NNVLLFKCELGAGHFSKSGRFEKL 703

BLAST of Spg010295 vs. TAIR 10
Match: AT1G76140.1 (Prolyl oligopeptidase family protein )

HSP 1 Score: 118.6 bits (296), Expect = 2.2e-26
Identity = 87/301 (28.90%), Postives = 131/301 (43.52%), Query Frame = 0

Query: 490 SHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLSLLDR-GFVLAFAD 549
           S DGT+IP+ I+        G  P +L  YG +   +  S+ ++R+ L    G V  FA+
Sbjct: 522 SKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFAN 581

Query: 550 VRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGA 609
           +RGGG  G+  WH+ GS  +KQN   DFIS A +L   GY   ++L   G S GGLLVGA
Sbjct: 582 IRGGGEYGE-EWHKAGSLAKKQNCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGA 641

Query: 610 AINMHPDLFRAAILKV---------SFSHGLAYTLVSGSSEVPFLDICNTLLDPTLPLTI 669
            IN  PDL+  A+  V          F+ G A+T                          
Sbjct: 642 CINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWT-------------------------- 701

Query: 670 LDYEEFGNPQIRVQFKSILSYSPYDNISR--------RSCYPSMLVTASFHDARVGVWEA 729
               ++G  +   +F  ++ YSP  N+ R           YPS ++  + HD RV    +
Sbjct: 702 ---SDYGCSENEEEFHWLIKYSPLHNVKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHS 761

Query: 730 AKWVAKIRDTTCSHCSTS-----AILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLGT 768
            K +A ++   C+    S      I +  +  GH          +E A  Y+F+ K++  
Sbjct: 762 LKLLATLQHVLCTSLDNSPQMNPIIGRIEVKAGHGAGRPTQKMIDEAADRYSFMAKMVNA 792

BLAST of Spg010295 vs. TAIR 10
Match: AT1G76140.2 (Prolyl oligopeptidase family protein )

HSP 1 Score: 115.2 bits (287), Expect = 2.4e-25
Identity = 86/298 (28.86%), Postives = 130/298 (43.62%), Query Frame = 0

Query: 490 SHDGTRIPLTILYSPMTFQKGQSPGVLQGYGAYGEILDKSWCSNRLSLLDR-GFVLAFAD 549
           S DGT+IP+ I+        G  P +L  YG +   +  S+ ++R+ L    G V  FA+
Sbjct: 522 SKDGTKIPMFIVAKKDIKLDGSHPCLLYAYGGFNISITPSFSASRIVLSKHLGVVFCFAN 581

Query: 550 VRGGGGVGDSSWHRCGSGLEKQNSIHDFISCANFLTDNGYVHKNRLGSIGYSAGGLLVGA 609
           +RGGG  G+  WH+ GS  +KQN   DFIS A +L   GY   ++L   G S GGLLVGA
Sbjct: 582 IRGGGEYGE-EWHKAGSLAKKQNCFDDFISGAEYLVSAGYTQPSKLCIEGGSNGGLLVGA 641

Query: 610 AINMHPDLFRAAILKV---------SFSHGLAYTLVSGSSEVPFLDICNTLLDPTLPLTI 669
            IN  PDL+  A+  V          F+ G A+T                          
Sbjct: 642 CINQRPDLYGCALAHVGVMDMLRFHKFTIGHAWT-------------------------- 701

Query: 670 LDYEEFGNPQIRVQFKSILSYSPYDNISR--------RSCYPSMLVTASFHDARVGVWEA 729
               ++G  +   +F  ++ YSP  N+ R           YPS ++  + HD RV    +
Sbjct: 702 ---SDYGCSENEEEFHWLIKYSPLHNVKRPWEQQTDHLVQYPSTMLLTADHDDRVVPLHS 761

Query: 730 AKWVAKIRDTTCSHC--STSAILKTNMLGGHFGEGGLYGGCEETAYEYAFLIKVLGTS 768
            K +A +  T+  +       I +  +  GH          +E A  Y+F+ K++  S
Sbjct: 762 LKLLAHVLCTSLDNSPQMNPIIGRIEVKAGHGAGRPTQKMIDEAADRYSFMAKMVNAS 789

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022977225.10.0e+0076.26uncharacterized protein LOC111477597 [Cucurbita maxima][more]
XP_023550805.10.0e+0076.26uncharacterized protein LOC111808835, partial [Cucurbita pepo subsp. pepo][more]
XP_022957328.10.0e+0076.02uncharacterized protein LOC111458759 [Cucurbita moschata][more]
XP_022149039.10.0e+0077.13uncharacterized protein LOC111017556 [Momordica charantia][more]
KAG6601360.10.0e+0078.40Protease 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q595363.7e-7126.05Protease 2 OS=Moraxella lacunata OX=477 GN=ptrB PE=3 SV=1[more]
P245553.0e-6525.73Protease 2 OS=Escherichia coli (strain K12) OX=83333 GN=ptrB PE=1 SV=2[more]
O078347.0e-6226.72Dipeptidyl aminopeptidase BI OS=Pseudoxanthomonas mexicana OX=128785 GN=dapb1 PE... [more]
Q32N481.6e-4228.30Prolyl endopeptidase-like OS=Xenopus laevis OX=8355 GN=prepl PE=2 SV=1[more]
P556566.8e-4122.60Uncharacterized peptidase y4sO OS=Sinorhizobium fredii (strain NBRC 101917 / NGR... [more]
Match NameE-valueIdentityDescription
A0A6J1ILQ30.0e+0076.26Prolyl endopeptidase OS=Cucurbita maxima OX=3661 GN=LOC111477597 PE=3 SV=1[more]
A0A6J1GZW20.0e+0076.02Prolyl endopeptidase OS=Cucurbita moschata OX=3662 GN=LOC111458759 PE=3 SV=1[more]
A0A6J1D5U20.0e+0077.13Prolyl endopeptidase OS=Momordica charantia OX=3673 GN=LOC111017556 PE=3 SV=1[more]
A0A0A0KQZ90.0e+0074.97Prolyl endopeptidase OS=Cucumis sativus OX=3659 GN=Csa_5G606880 PE=3 SV=1[more]
A0A1S3BF530.0e+0073.68Prolyl endopeptidase OS=Cucumis melo OX=3656 GN=LOC103489202 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G69020.11.0e-23351.98Prolyl oligopeptidase family protein [more]
AT5G66960.14.5e-12534.91Prolyl oligopeptidase family protein [more]
AT1G50380.19.9e-8027.96Prolyl oligopeptidase family protein [more]
AT1G76140.12.2e-2628.90Prolyl oligopeptidase family protein [more]
AT1G76140.22.4e-2528.86Prolyl oligopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002470Peptidase S9A, prolyl oligopeptidasePRINTSPR00862PROLIGOPTASEcoord: 513..531
score: 31.86
coord: 598..618
score: 59.65
coord: 539..563
score: 36.42
coord: 568..587
score: 37.63
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 448..768
e-value: 6.8E-75
score: 254.3
coord: 41..118
e-value: 4.7E-12
score: 47.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 485..721
IPR023302Peptidase S9A, N-terminal domainPFAMPF02897Peptidase_S9_Ncoord: 254..427
e-value: 5.2E-6
score: 25.5
coord: 44..182
e-value: 2.0E-21
score: 76.3
NoneNo IPR availableGENE3D2.130.10.120coord: 252..434
e-value: 3.1E-27
score: 97.5
NoneNo IPR availableGENE3D2.130.10.120coord: 119..251
e-value: 1.4E-24
score: 88.6
NoneNo IPR availablePANTHERPTHR11757:SF12PROLYL OLIGOPEPTIDASE FAMILY PROTEINcoord: 238..767
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 238..767
NoneNo IPR availablePANTHERPTHR11757PROTEASE FAMILY S9A OLIGOPEPTIDASEcoord: 36..243
NoneNo IPR availablePANTHERPTHR11757:SF12PROLYL OLIGOPEPTIDASE FAMILY PROTEINcoord: 36..243
NoneNo IPR availableSUPERFAMILY50993Peptidase/esterase 'gauge' domaincoord: 40..436
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 532..633
e-value: 5.4E-27
score: 94.7
coord: 639..765
e-value: 2.9E-15
score: 56.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg010295.1Spg010295.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity