Spg010129 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg010129
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4
Location	scaffold7: 5718486 .. 5721679 (-)
RNA-Seq Expression	Spg010129
Synteny	Spg010129

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCGTTTGGACTCTTCAAGTCAACTAAAGAGTCTCATCTTTAATTTCAAAAATCGTCTATATATTTTCCCACACACAACTCCTTCAATTAACAAACCAAACCTCAAAATCCCCGACCTCTTTTTTCCAACAATCCACCTCTAAAAATGGTCGCCGCCGGTCCGGGAGGCGGCGGAGGAATCTCCAGCTGCTCCGACATACGAACCATCGCCGTCCACGTCATCACCGGGCGGTGGTTCGTGGTGTTCGCTTCACTTCTGATCATGGCGGCCGCCGGCGCCACCTACATGTTCAGCCTGTATTCCAACGACATAAAGTCAGTTTTGGGGTACGATCAAACCACGTTGAATTTGTTGAGCTTTTTCAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCCGGTCTTATCAATGAAGTTACGCCGCCGTGGGTGGTTCTCTCCATCGGCGCCGTCCTCAATTTCTTTGGTTACTTCATGATTTGGCTCGCCGTCACTAAGAGGATCTCCACCCCCAAAGTTTGGCAAATGTGTCTCTATATTTGTGTTGGAGCTAATTCTCAGTCCTTCGCCAACACCGGTTCCCTTGTTACTTGCGTCAAGAACTTCCGCGAAAGTCGGGGCGTCGTTCTCGGTGAGTGATTATCAATATTAATTGTTGAAAAATTGACATTTTTGCTGTCTAGTGTTTTCATGTATATATCTTTTGAATTTCAAAAGATATACTCACTTTTAACTATGTTTAGATTGACTGTTATGTCAAATTAGTTCGTATATTCAAAAGTTTAAGCTTATATAACCTCAACCATTTTCCTTTTGGGTTTGTGAGAAAGAATAAAAATTATCTACATATCAATAGATTCAATATTACCATACCATGAAATGATTTGACAACAATTCTTGACAACTAGGAGAAATAAAAAAGTTTCATCATTTTCTCCCATCTTCTTTCTTAGTTGTCAAAATTAAATCTGTTAGATAAGTTTTTTTTTTGGTTCAAAGAGATTCGAACCTCTGATCTTTTAGTCATAGTATATGTCAATTACCGTTAACTATGTCCACTTTGACTTGTTAGATAAATATTGTTAGTTTGAAAATTAGCCAAACACTCAAGAGTGTATTCTTTTTTTCCTCAAAATATTTACATGAGACTTGTGTGATGTTTTGGGTAGAGAGTTTGAACAGGTTTGGCATAAGCCATTGGACTCATACTTAATTTGTTTTCCTGAATCCAAGGTTTCCTTAATCTAGATTTTCTAAACTCTCCATGATTTAAACTATATATATATATATTATTTTCATCCCAAACTCATAGAGATTCGAACACTATATTGTATTCTATATTAAATTACTATTCAGCTCTGAAACTTTTTTTTTTTTACAATAGGTGGGATATGAGATTCGAACCACAGACCTCATGGTCATTAATATAACTTCAATTGAGCTATGTTTTGTTCGCTATTCAACTCCAAAACTTAAACATAAATTTAATCATTTATTCATGTTTTGAACAGGTATCTTAAAGGGCTACGTCGGGCTTAGCGGCGCCATAATCACACAACTCTTCCACGCCTTCTACGGCTCCGACACCAAATCCTTAATCCTCCTCATCGGATGGCTCCCGGCGGCCATTTCCTTCGCATTTCTCCGAACAATTCGGATCATGAAAGTTATCCGACAGCCCAACGAGCTCAAAGTCTTCTACAATTTCCTCTACATTTCTCTCGCCCTCGCCGGATTCCTAATGCTAATGATCATCATAGAAAGCAAACTAATGTTCACTCAAAGCGAATTCGGCGGCAGCGCCGCCGTCGTCCTCCTCCTCCTCTTCCTCCCCCTCGCCGTCGTAGTAATCGAAGAATACAACCTCTGGAAACTCAAAACCGCCGCCATTGGAGCTCCAAATCCAGTCCAGATCATAACAGAGAAGCCAAACATCCCAAAAATAGAGCATCAGAAACAAACCCAACAAAAACAAAAACAAAAGCAGCCATCTTGCTGGACGACGATCTTCAGCCCGCCGGAACGCGGCGAGGATTTCACGATTCTCCAAGCTCTCTTCAGCATGGACATGTTGATTCTCTTCTTCTCTGCAATCTGCGGCGTCGGCGGCACGTTGACGGCGATCGACAATCTGGGTCAGATCGGAATGTCTTTAGGATATCCAAAAAGAAGCATCAGCACATTTGTGACACTCGTGAGCATTTGGAATTACCTCGGCCGAGTCGCCTCTGGTTTCGTCTCTGAAATTGTTCTAACCAAATACAAATTCCCTCGTCCTTTGATGCTCTCTTTGACTCTGCTTTTGTCCTGCGTCGGCCATTTGATGATCGCTTTCGATGTCCCAAATGGGCTTTACGTGGCTTCGATCGTGATCGGATTCTGCTTCGGCGCGCAGTGGCCGTTGCTTTTCGCTATAATTTCGGAGCTTTTCGGGTTGAAGTATTACTCCACGCTTTACAATTTCGGGTCAGTGGCGAGCCCGATTGGGCTTTATGTTCTGAATGTGAAAGTGGCGGGGAATTTCTACGACAGAGAGGCGGAGAAGCAGCTGATAGCAAAAGGACTTGTGAGAAAAGCAGGGGAAGAGCTGAAGTGCTTTGGAGCAGAGTGCTTTAAGCTGTCGTTTATTGTGATTACGGGAGTTACTTTGTTGGGTATGCTTGTTTCTTTGATTTTAGTGATCAGAACGAGGAGTTTTTACAAGAGTGATATTTACAAGAAGTTCAGAGAGGAAGCTGAAGCAGAGGTTGCCGGAAATGGCGCGGTGGTGGCCGCCGAGAGATAAAGAGAAATGTTTGTGATTTTGGGGAGAGAGATTTTTCTGATCTCTTGGCTTGTTTGAAGAAACAATGTTTGTTCTTTGTGGACAAGAGTATAATTTAAGCACACTTGGAAGAAAAAGTTAAGAGAAGTTTTTTTTTTTGAACCAAAGTTAAGAGAAGTTACAAGTTCCATGATAAAGTTTTAAGGGATATTTTGATTATGTTATGTGTATCCCATTATTGTAATTTATAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTGACAACATGTGATCGAGGTGGAGGATTTGAACCACAGATCTCTTGGTCATTGTTAAAAGTTTTATGCCAGTTGAATTGGCTTATGTTAGCATTGGAATTTACAAGTTTCTAATATAGTCCTATCATTTTCAATTTTATGTGTTATATATTT

mRNA sequence

ATGGTCGCCGCCGGTCCGGGAGGCGGCGGAGGAATCTCCAGCTGCTCCGACATACGAACCATCGCCGTCCACGTCATCACCGGGCGGTGGTTCGTGGTGTTCGCTTCACTTCTGATCATGGCGGCCGCCGGCGCCACCTACATGTTCAGCCTGTATTCCAACGACATAAAGTCAGTTTTGGGGTACGATCAAACCACGTTGAATTTGTTGAGCTTTTTCAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCCGGTCTTATCAATGAAGTTACGCCGCCGTGGGTGGTTCTCTCCATCGGCGCCGTCCTCAATTTCTTTGGTTACTTCATGATTTGGCTCGCCGTCACTAAGAGGATCTCCACCCCCAAAGTTTGGCAAATGTGTCTCTATATTTGTGTTGGAGCTAATTCTCAGTCCTTCGCCAACACCGGTTCCCTTGTTACTTGCGTCAAGAACTTCCGCGAAAGTCGGGGCGTCGTTCTCGGTATCTTAAAGGGCTACGTCGGGCTTAGCGGCGCCATAATCACACAACTCTTCCACGCCTTCTACGGCTCCGACACCAAATCCTTAATCCTCCTCATCGGATGGCTCCCGGCGGCCATTTCCTTCGCATTTCTCCGAACAATTCGGATCATGAAAGTTATCCGACAGCCCAACGAGCTCAAAGTCTTCTACAATTTCCTCTACATTTCTCTCGCCCTCGCCGGATTCCTAATGCTAATGATCATCATAGAAAGCAAACTAATGTTCACTCAAAGCGAATTCGGCGGCAGCGCCGCCGTCGTCCTCCTCCTCCTCTTCCTCCCCCTCGCCGTCGTAGTAATCGAAGAATACAACCTCTGGAAACTCAAAACCGCCGCCATTGGAGCTCCAAATCCAGTCCAGATCATAACAGAGAAGCCAAACATCCCAAAAATAGAGCATCAGAAACAAACCCAACAAAAACAAAAACAAAAGCAGCCATCTTGCTGGACGACGATCTTCAGCCCGCCGGAACGCGGCGAGGATTTCACGATTCTCCAAGCTCTCTTCAGCATGGACATGTTGATTCTCTTCTTCTCTGCAATCTGCGGCGTCGGCGGCACGTTGACGGCGATCGACAATCTGGGTCAGATCGGAATGTCTTTAGGATATCCAAAAAGAAGCATCAGCACATTTGTGACACTCGTGAGCATTTGGAATTACCTCGGCCGAGTCGCCTCTGGTTTCGTCTCTGAAATTGTTCTAACCAAATACAAATTCCCTCGTCCTTTGATGCTCTCTTTGACTCTGCTTTTGTCCTGCGTCGGCCATTTGATGATCGCTTTCGATGTCCCAAATGGGCTTTACGTGGCTTCGATCGTGATCGGATTCTGCTTCGGCGCGCAGTGGCCGTTGCTTTTCGCTATAATTTCGGAGCTTTTCGGGTTGAAGTATTACTCCACGCTTTACAATTTCGGGTCAGTGGCGAGCCCGATTGGGCTTTATGTTCTGAATGTGAAAGTGGCGGGGAATTTCTACGACAGAGAGGCGGAGAAGCAGCTGATAGCAAAAGGACTTGTGAGAAAAGCAGGGGAAGAGCTGAAGTGCTTTGGAGCAGAGTGCTTTAAGCTGTCGTTTATTGTGATTACGGGAGTTACTTTGTTGGGTATGCTTGTTTCTTTGATTTTAGTGATCAGAACGAGGAGTTTTTACAAGAGTGATATTTACAAGAAGTTCAGAGAGGAAGCTGAAGCAGAGGTTGCCGGAAATGGCGCGGTGGTGGCCGCCGAGAGATAA

Coding sequence (CDS)

ATGGTCGCCGCCGGTCCGGGAGGCGGCGGAGGAATCTCCAGCTGCTCCGACATACGAACCATCGCCGTCCACGTCATCACCGGGCGGTGGTTCGTGGTGTTCGCTTCACTTCTGATCATGGCGGCCGCCGGCGCCACCTACATGTTCAGCCTGTATTCCAACGACATAAAGTCAGTTTTGGGGTACGATCAAACCACGTTGAATTTGTTGAGCTTTTTCAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCCGGTCTTATCAATGAAGTTACGCCGCCGTGGGTGGTTCTCTCCATCGGCGCCGTCCTCAATTTCTTTGGTTACTTCATGATTTGGCTCGCCGTCACTAAGAGGATCTCCACCCCCAAAGTTTGGCAAATGTGTCTCTATATTTGTGTTGGAGCTAATTCTCAGTCCTTCGCCAACACCGGTTCCCTTGTTACTTGCGTCAAGAACTTCCGCGAAAGTCGGGGCGTCGTTCTCGGTATCTTAAAGGGCTACGTCGGGCTTAGCGGCGCCATAATCACACAACTCTTCCACGCCTTCTACGGCTCCGACACCAAATCCTTAATCCTCCTCATCGGATGGCTCCCGGCGGCCATTTCCTTCGCATTTCTCCGAACAATTCGGATCATGAAAGTTATCCGACAGCCCAACGAGCTCAAAGTCTTCTACAATTTCCTCTACATTTCTCTCGCCCTCGCCGGATTCCTAATGCTAATGATCATCATAGAAAGCAAACTAATGTTCACTCAAAGCGAATTCGGCGGCAGCGCCGCCGTCGTCCTCCTCCTCCTCTTCCTCCCCCTCGCCGTCGTAGTAATCGAAGAATACAACCTCTGGAAACTCAAAACCGCCGCCATTGGAGCTCCAAATCCAGTCCAGATCATAACAGAGAAGCCAAACATCCCAAAAATAGAGCATCAGAAACAAACCCAACAAAAACAAAAACAAAAGCAGCCATCTTGCTGGACGACGATCTTCAGCCCGCCGGAACGCGGCGAGGATTTCACGATTCTCCAAGCTCTCTTCAGCATGGACATGTTGATTCTCTTCTTCTCTGCAATCTGCGGCGTCGGCGGCACGTTGACGGCGATCGACAATCTGGGTCAGATCGGAATGTCTTTAGGATATCCAAAAAGAAGCATCAGCACATTTGTGACACTCGTGAGCATTTGGAATTACCTCGGCCGAGTCGCCTCTGGTTTCGTCTCTGAAATTGTTCTAACCAAATACAAATTCCCTCGTCCTTTGATGCTCTCTTTGACTCTGCTTTTGTCCTGCGTCGGCCATTTGATGATCGCTTTCGATGTCCCAAATGGGCTTTACGTGGCTTCGATCGTGATCGGATTCTGCTTCGGCGCGCAGTGGCCGTTGCTTTTCGCTATAATTTCGGAGCTTTTCGGGTTGAAGTATTACTCCACGCTTTACAATTTCGGGTCAGTGGCGAGCCCGATTGGGCTTTATGTTCTGAATGTGAAAGTGGCGGGGAATTTCTACGACAGAGAGGCGGAGAAGCAGCTGATAGCAAAAGGACTTGTGAGAAAAGCAGGGGAAGAGCTGAAGTGCTTTGGAGCAGAGTGCTTTAAGCTGTCGTTTATTGTGATTACGGGAGTTACTTTGTTGGGTATGCTTGTTTCTTTGATTTTAGTGATCAGAACGAGGAGTTTTTACAAGAGTGATATTTACAAGAAGTTCAGAGAGGAAGCTGAAGCAGAGGTTGCCGGAAATGGCGCGGTGGTGGCCGCCGAGAGATAA

Protein sequence

MVAAGPGGGGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMCLYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKLMFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEKPNIPKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAAER

Homology

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BLAST of Spg010129 vs. NCBI nr
Match: KAG6573851.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1031.6 bits (2666), Expect = 2.7e-297
Identity = 538/583 (92.28%), Postives = 557/583 (95.54%), Query Frame = 0


Query: 1   MVAAGPGGGGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60
           MVAAG GG G IS  S+IRTIAVH+ITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL
Sbjct: 1   MVAAGQGGDGAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVG+LSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGILSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 TPKVWQMCLYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFH 180
           TPKVWQMCLYICVGANSQSFANTGSLVTCVKNF +SRGVVLGILKGYVGLSGAIITQLFH
Sbjct: 121 TPKVWQMCLYICVGANSQSFANTGSLVTCVKNFPQSRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240
           AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 MIIIESKLMFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEK 300
           MIIIESKL+F+Q+EFGGSAAVVLLLLFLPLAVV++EEYNL KLK+AAI  PNP+QI+TEK
Sbjct: 241 MIIIESKLIFSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 PNIPKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVG 360
           PN PK E QKQT+Q   QKQPSCWTTIFSPPERGEDFTILQALFS+DM ILFFSAICGVG
Sbjct: 301 PNPPKTEPQKQTRQ---QKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVG 360

Query: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420
           GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML
Sbjct: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420

Query: 421 SLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480
           SLTLLLSCVGHL+IAF VPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG
Sbjct: 421 SLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480

Query: 481 SVASPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVT 540
           SVASPIGLYVLNVKVAGN YD+EA KQL AKGLVRK GEELKC+G ECFK SF+VITGVT
Sbjct: 481 SVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVT 540

Query: 541 LLGMLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAA 584
           LLGM VSLILVIRTR FYKSDIYKKFR+EAEAEVAGNGAV AA
Sbjct: 541 LLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVAGNGAVAAA 580

[Close]

BLAST of Spg010129 vs. NCBI nr
Match: XP_022944943.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita moschata])

HSP 1 Score: 1026.9 bits (2654), Expect = 6.6e-296
Identity = 537/583 (92.11%), Postives = 554/583 (95.03%), Query Frame = 0


Query: 1   MVAAGPGGGGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60
           MVAAG GG   IS  S+IRTIAVH+ITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL
Sbjct: 1   MVAAGQGGDRAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 TPKVWQMCLYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFH 180
           TPKVWQMCLYICVGANSQSF NTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFH
Sbjct: 121 TPKVWQMCLYICVGANSQSFVNTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240
           AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 MIIIESKLMFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEK 300
           MIIIESKL+F+Q+EFGGSAAVVLLLLFLPLAVV++EEYNL KLK+AAI  PNP+QI+TEK
Sbjct: 241 MIIIESKLIFSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 PNIPKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVG 360
           PN PK E QKQT+Q   QKQPSCWTTIFSPPERGEDFTILQALFS+DM ILFFSAICGVG
Sbjct: 301 PNPPKTEPQKQTRQ---QKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVG 360

Query: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420
           GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML
Sbjct: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420

Query: 421 SLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480
           SLTLLLSCVGHL+IAF VPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG
Sbjct: 421 SLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480

Query: 481 SVASPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVT 540
           SVASPIGLYVLNVKVAGN YD+EA KQL AKGLVRK GEELKC+G ECFK SF+VITGVT
Sbjct: 481 SVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVT 540

Query: 541 LLGMLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAA 584
           LLGM VSLILVIRTR FYKSDIYKKFR+EAEAEV GNGAV AA
Sbjct: 541 LLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVVGNGAVTAA 580

[Close]

BLAST of Spg010129 vs. NCBI nr
Match: XP_023541417.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1015.8 bits (2625), Expect = 1.5e-292
Identity = 530/583 (90.91%), Postives = 549/583 (94.17%), Query Frame = 0


Query: 1   MVAAGPGGGGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60
           MVA G GG G IS  S+IRT+AVH+ITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL
Sbjct: 1   MVAPGQGGDGAISGGSNIRTLAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 TPKVWQMCLYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFH 180
           TPKVWQMCLYIC+GANSQSFANTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFH
Sbjct: 121 TPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240
           AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 MIIIESKLMFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEK 300
           MIIIESKL+FTQ+EFGGSAAVVLLLLFLPLAVV++EEYNL KLK+AAI  PNP+QI+TEK
Sbjct: 241 MIIIESKLIFTQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 PNIPKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVG 360
           PN PK E          +KQPSCWTTIF+PPERGEDFTILQALFS+DM ILFFSAICGVG
Sbjct: 301 PNPPKTE---------PRKQPSCWTTIFNPPERGEDFTILQALFSLDMAILFFSAICGVG 360

Query: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420
           GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML
Sbjct: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420

Query: 421 SLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480
           SLTLLLSCVGHL+IAF VPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG
Sbjct: 421 SLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480

Query: 481 SVASPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVT 540
           SVASPIGLYVLNVKVAGN YD+EA KQL AKGLVRK GEELKC+G ECFK SF+VITGVT
Sbjct: 481 SVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVT 540

Query: 541 LLGMLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAA 584
           LLGM VSLILVIRTR FYKSDIYKKFR+EAEAEV GNGAV AA
Sbjct: 541 LLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVVGNGAVAAA 574

[Close]

BLAST of Spg010129 vs. NCBI nr
Match: XP_022956604.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 1005.7 bits (2599), Expect = 1.6e-289
Identity = 522/580 (90.00%), Postives = 550/580 (94.83%), Query Frame = 0


Query: 9   GGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLN 68
           GGGIS CSDI +IAVHVITGRWFVVFASLLIMAAAGATYMFSLYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAV+NFFGYFMIWLAVT++I+ PKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVWQMC 123

Query: 129 LYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTK 188
           LYIC+GANSQSFANTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFHAFYG DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTK 183

Query: 189 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKT 243

Query: 249 MFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLK----TAAIGAPN-PVQIITEKPNI 308
            F+QS +GGSAAVVLLLLFLPLAVV+IEEYNLW LK    TAAI +PN PVQI+TEK  +
Sbjct: 244 EFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK--L 303

Query: 309 PKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTL 368
           PK EHQK   + + QK+PSCWTTIF+PPERGEDFTILQALFS+DMLILF +AICGVGGTL
Sbjct: 304 PKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGTL 363

Query: 369 TAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLT 428
           TAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRV SGFVSEIVLTKYKFPRPLMLS+T
Sbjct: 364 TAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMT 423

Query: 429 LLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVA 488
           LL+SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPL+FAIISELFGLKYYSTLYNFGSVA
Sbjct: 424 LLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVA 483

Query: 489 SPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLG 548
           SPIGLYVLNVKVAGNFYDREAEKQL AKG+VRK GEELKCFG ECFKLSF+VITGVTLLG
Sbjct: 484 SPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLG 543

Query: 549 MLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAA 584
           M VSLILVIRTRSFYKSDIYKKFR+EAE E+AGNGAVVAA
Sbjct: 544 MFVSLILVIRTRSFYKSDIYKKFRDEAETEMAGNGAVVAA 581

[Close]

BLAST of Spg010129 vs. NCBI nr
Match: XP_023535761.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.7e-289
Identity = 521/580 (89.83%), Postives = 549/580 (94.66%), Query Frame = 0


Query: 9   GGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLN 68
           GGG S CSDI +IAVHVITGRWFVVFASLLIMAAAGATYMFSLYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGTSGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAV+NFFGYFMIWLAVT++IS PKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTQKISAPKVWQMC 123

Query: 129 LYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTK 188
           LYIC+GANSQSFANTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFHAFYG DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTK 183

Query: 189 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKT 243

Query: 249 MFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLK----TAAIGAPN-PVQIITEKPNI 308
            F+QS +GGSAAVVLLLLFLPLAVV+IEEYNLW LK    TAAI +PN PVQI+TEK  +
Sbjct: 244 EFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAATTAAIRSPNHPVQIVTEK--V 303

Query: 309 PKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTL 368
           PK EHQK   + + +KQPSCWTTIF+PPERGEDFTILQALFS+DMLILF +AICGVGGTL
Sbjct: 304 PKTEHQKHIPKTEHKKQPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGTL 363

Query: 369 TAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLT 428
           TAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRV SGFVSEIVLTKYKFPRPLMLS+T
Sbjct: 364 TAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMT 423

Query: 429 LLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVA 488
           LL+SCVGHLMIAFDVPNGLYVAS+VIGFCFGAQWPL+FAIISELFGLKYYSTLYNFGSVA
Sbjct: 424 LLVSCVGHLMIAFDVPNGLYVASVVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVA 483

Query: 489 SPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLG 548
           SPIGLYVLNVKVAGNFYDREAEKQL AKG+VRK GEELKCFG ECFKLSF+VITGVTLLG
Sbjct: 484 SPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLG 543

Query: 549 MLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAA 584
           M VSLILVIRTRSFYKSDIYKKFR+EAE E+AGNGAVVAA
Sbjct: 544 MFVSLILVIRTRSFYKSDIYKKFRDEAETEIAGNGAVVAA 581

[Close]

BLAST of Spg010129 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.9e-29
Identity = 135/540 (25.00%), Postives = 248/540 (45.93%), Query Frame = 0


Query: 29  RWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLIN 88
           +W V+ A++ I A+ G  + FS YS+ +KSVLG  Q  LN L+   DLG   G  SG+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 89  EVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMCLYICVGAN-SQSFANTGSLV 148
              P  VVL   A + F GY + WL +T  I+ P  + +    C+ A  S  + NT   +
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLSICWFNTACFI 162

Query: 149 TCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAISFAFLR 208
            C+++F  +R + L +   + G+S A+ +  F+A   S +   +LL   +P  +SFA L 
Sbjct: 163 LCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAAL- 222

Query: 209 TIRIMKVIRQPN-ELKVFYNFLYISLALAGFLMLMIIIESKLMFTQSEFGGSA------A 268
                 V+ +P+ +    Y+       +   L ++ +I S  +   S    SA      A
Sbjct: 223 ----YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGA 282

Query: 269 VVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEKPNIPKIEHQKQTQQKQKQKQ 328
           VVLL+  L   ++V        +  A +   +   ++    NI ++++QK +   +   +
Sbjct: 283 VVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVML---NIDELKNQKTSVSSKTGYE 342

Query: 329 PSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTLTAIDNLGQIGMSLGYPK 388
                   +    G++ +    +  ++  + + +  CG    L   +NLGQI  SLG   
Sbjct: 343 HMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG--- 402

Query: 389 RSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLSCVGHLMIAF--DV 448
           ++ +T VT+ S +++ GR+ S    + +  +++  R    ++ LL + +   ++A     
Sbjct: 403 QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQ 462

Query: 449 PNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGN 508
              L  A+ +IG   G  +    +I S+LFG       +N      PIG  +L   +A +
Sbjct: 463 QTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIG-SLLYGYIAAS 522

Query: 509 FYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVSLILVIRTRSFY 559
            Y+  A     +  +     + + C G +C+  +F+    +++LG++ SL L IRT+  Y
Sbjct: 523 IYEANA-----SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

[Close]

BLAST of Spg010129 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 4.6e-05
Identity = 57/229 (24.89%), Postives = 100/229 (43.67%), Query Frame = 0


Query: 334 GEDFTILQALFSMDMLILFFSAICGVGGTLTAI-DNLGQIGMSL---GYPKRSISTFVTL 393
           G +   L+  F+     LF      +GG      +N+G I  ++        S ST V+L
Sbjct: 259 GHEGATLKEFFTDKTAWLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSL 318

Query: 394 VSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLSCVGHLMI------AFDVPNGLY 453
            + ++ + R+  GF SE +  +    RP++LS+  L++   HLM+       FD      
Sbjct: 319 FATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVIALVAACIHLMVPSGIFTVFDNAKYFS 378

Query: 454 VASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDRE 513
           V +IV GF +G+ + L+  I+++++G+    T++         G ++L + V    Y   
Sbjct: 379 VVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW---------GSFILALAVGSLGYGLL 438

Query: 514 AEKQLIAKGLVRKAGEELKCFGAECFKLSFIVI-TGVTLLGMLVSLILV 552
             K   A   V        C G  C+ L+F++  TG+      V  I V
Sbjct: 439 FAKVYDAASEVGVGSMSQVCSGVHCYGLTFVITGTGLAFAAAAVFFIWV 476

[Close]

BLAST of Spg010129 vs. ExPASy TrEMBL
Match: A0A6J1FZF2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326 PE=4 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 3.2e-296
Identity = 537/583 (92.11%), Postives = 554/583 (95.03%), Query Frame = 0


Query: 1   MVAAGPGGGGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60
           MVAAG GG   IS  S+IRTIAVH+ITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL
Sbjct: 1   MVAAGQGGDRAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 TPKVWQMCLYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFH 180
           TPKVWQMCLYICVGANSQSF NTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFH
Sbjct: 121 TPKVWQMCLYICVGANSQSFVNTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240
           AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 MIIIESKLMFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEK 300
           MIIIESKL+F+Q+EFGGSAAVVLLLLFLPLAVV++EEYNL KLK+AAI  PNP+QI+TEK
Sbjct: 241 MIIIESKLIFSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 PNIPKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVG 360
           PN PK E QKQT+Q   QKQPSCWTTIFSPPERGEDFTILQALFS+DM ILFFSAICGVG
Sbjct: 301 PNPPKTEPQKQTRQ---QKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVG 360

Query: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420
           GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML
Sbjct: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420

Query: 421 SLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480
           SLTLLLSCVGHL+IAF VPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG
Sbjct: 421 SLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480

Query: 481 SVASPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVT 540
           SVASPIGLYVLNVKVAGN YD+EA KQL AKGLVRK GEELKC+G ECFK SF+VITGVT
Sbjct: 481 SVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVT 540

Query: 541 LLGMLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAA 584
           LLGM VSLILVIRTR FYKSDIYKKFR+EAEAEV GNGAV AA
Sbjct: 541 LLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVVGNGAVTAA 580

[Close]

BLAST of Spg010129 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 7.7e-290
Identity = 522/580 (90.00%), Postives = 550/580 (94.83%), Query Frame = 0


Query: 9   GGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLN 68
           GGGIS CSDI +IAVHVITGRWFVVFASLLIMAAAGATYMFSLYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAV+NFFGYFMIWLAVT++I+ PKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVWQMC 123

Query: 129 LYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTK 188
           LYIC+GANSQSFANTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFHAFYG DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTK 183

Query: 189 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKT 243

Query: 249 MFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLK----TAAIGAPN-PVQIITEKPNI 308
            F+QS +GGSAAVVLLLLFLPLAVV+IEEYNLW LK    TAAI +PN PVQI+TEK  +
Sbjct: 244 EFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK--L 303

Query: 309 PKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTL 368
           PK EHQK   + + QK+PSCWTTIF+PPERGEDFTILQALFS+DMLILF +AICGVGGTL
Sbjct: 304 PKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGTL 363

Query: 369 TAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLT 428
           TAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRV SGFVSEIVLTKYKFPRPLMLS+T
Sbjct: 364 TAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMT 423

Query: 429 LLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVA 488
           LL+SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPL+FAIISELFGLKYYSTLYNFGSVA
Sbjct: 424 LLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVA 483

Query: 489 SPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLG 548
           SPIGLYVLNVKVAGNFYDREAEKQL AKG+VRK GEELKCFG ECFKLSF+VITGVTLLG
Sbjct: 484 SPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLG 543

Query: 549 MLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAA 584
           M VSLILVIRTRSFYKSDIYKKFR+EAE E+AGNGAVVAA
Sbjct: 544 MFVSLILVIRTRSFYKSDIYKKFRDEAETEMAGNGAVVAA 581

[Close]

BLAST of Spg010129 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 9.4e-288
Identity = 523/582 (89.86%), Postives = 545/582 (93.64%), Query Frame = 0


Query: 9   GGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLN 68
           GGGIS CSDI +IAVHVITGRWFVVFASLLIMAAAGATYMFSLYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAV+NFFGYFMIWLAVT +IS PKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVWQMC 123

Query: 129 LYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTK 188
           LYIC+GANSQSFANTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFHAFYG DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTK 183

Query: 189 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESK 
Sbjct: 184 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKT 243

Query: 249 MFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKT-----AAIGAPN-PVQIITEKPN 308
            F+QS +GGSAAVVLLLLFLPLAVV+IEEYNLW LKT     AAI +PN PVQI+TEK  
Sbjct: 244 EFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAAIRSPNHPVQIVTEK-- 303

Query: 309 IPKIEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGT 368
           +PK EH         QKQPSCWTTIF+PPERGEDFTILQALFS+DM ILF +AICGVGGT
Sbjct: 304 VPKTEH---------QKQPSCWTTIFNPPERGEDFTILQALFSVDMFILFLAAICGVGGT 363

Query: 369 LTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSL 428
           LTAIDNLGQIGMSLGYPK+SISTFVTLVSIWNYLGRV SGFVSEIVLTKYKFPRPLMLS+
Sbjct: 364 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSM 423

Query: 429 TLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSV 488
           TLL+SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPL+FAIISELFGLKYYSTLYNFGSV
Sbjct: 424 TLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 483

Query: 489 ASPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLL 548
           ASPIGLYVLNVKVAGNFYDREAEKQL AKG+VRK GEELKCFG ECFKLSF+VITGVTLL
Sbjct: 484 ASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLL 543

Query: 549 GMLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAAE 585
           GM VSLILVIRTRSFYKSDIYKKFR+EAE E+AGNGAVVAAE
Sbjct: 544 GMFVSLILVIRTRSFYKSDIYKKFRDEAETEIAGNGAVVAAE 574

[Close]

BLAST of Spg010129 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 6.1e-287
Identity = 517/577 (89.60%), Postives = 546/577 (94.63%), Query Frame = 0


Query: 9   GGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLN 68
           GGGIS CSDI +IAVHVITGRWFVVFASLLIMAAAGATYMF LYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVT+RISTPKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMC 123

Query: 129 LYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTK 188
           LYIC+GANSQSFANTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFHAFYG+DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTK 183

Query: 189 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILLIGWLPAAISFA LRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKK 243

Query: 249 MFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNP-VQIITEKPNIPKIE 308
            F Q+E+GGSAAVVLLLLFLPL+VV+IEEYNLWKLKTA + +PNP VQI+TEK  +PK E
Sbjct: 244 EFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEK--LPKTE 303

Query: 309 HQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTLTAID 368
           H KQ +    QK+PSCWTTIF+PP+RGEDFTILQALFS DMLILF +AICGVGGTLTAID
Sbjct: 304 HPKQLE----QKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAID 363

Query: 369 NLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLS 428
           NLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPL+LSLTLLLS
Sbjct: 364 NLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLS 423

Query: 429 CVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASPIG 488
           CVGHL+IAFDVPNGLYVASIVIGFCFGAQWPL+FAIISELFGLKYYSTLYNFGSVASPIG
Sbjct: 424 CVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIG 483

Query: 489 LYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVS 548
           LY+LNVKVAGNFYDREAEKQL AKG++RKAGEELKCFG ECFKLSFIVIT VTLLGMLVS
Sbjct: 484 LYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVS 543

Query: 549 LILVIRTRSFYKSDIYKKFREEAEA-EVAGNGAVVAA 584
           L+LVIRTRSFYKSDIYKKFR+EAE  EVAGN  +  A
Sbjct: 544 LVLVIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVA 574

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BLAST of Spg010129 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 6.1e-287
Identity = 517/577 (89.60%), Postives = 546/577 (94.63%), Query Frame = 0


Query: 9   GGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLN 68
           GGGIS CSDI +IAVHVITGRWFVVFASLLIMAAAGATYMF LYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVT+RISTPKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMC 123

Query: 129 LYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTK 188
           LYIC+GANSQSFANTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQLFHAFYG+DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTK 183

Query: 189 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILLIGWLPAAISFA LRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKK 243

Query: 249 MFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNP-VQIITEKPNIPKIE 308
            F Q+E+GGSAAVVLLLLFLPL+VV+IEEYNLWKLKTA + +PNP VQI+TEK  +PK E
Sbjct: 244 EFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEK--LPKTE 303

Query: 309 HQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTLTAID 368
           H KQ +    QK+PSCWTTIF+PP+RGEDFTILQALFS DMLILF +AICGVGGTLTAID
Sbjct: 304 HPKQLE----QKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAID 363

Query: 369 NLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLS 428
           NLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPL+LSLTLLLS
Sbjct: 364 NLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLS 423

Query: 429 CVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASPIG 488
           CVGHL+IAFDVPNGLYVASIVIGFCFGAQWPL+FAIISELFGLKYYSTLYNFGSVASPIG
Sbjct: 424 CVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIG 483

Query: 489 LYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVS 548
           LY+LNVKVAGNFYDREAEKQL AKG++RKAGEELKCFG ECFKLSFIVIT VTLLGMLVS
Sbjct: 484 LYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVS 543

Query: 549 LILVIRTRSFYKSDIYKKFREEAEA-EVAGNGAVVAA 584
           L+LVIRTRSFYKSDIYKKFR+EAE  EVAGN  +  A
Sbjct: 544 LVLVIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVA 574

[Close]

BLAST of Spg010129 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 828.2 bits (2138), Expect = 4.2e-240
Identity = 424/588 (72.11%), Postives = 484/588 (82.31%), Query Frame = 0


Query: 1   MVAAGPGGGGGISSCSDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60
           MVAA PGG         ++++ + ++TGRWF+ F SLLIM+ AGATYMF +YS DIK  L
Sbjct: 1   MVAASPGG--------SMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVGVL+GL+NEVTPPW +L IGA+LNFFGYFMIWLAVT+RIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERIS 120

Query: 121 TPKVWQMCLYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFH 180
            P+VW MCLYICVGANSQSFANTGSLVTCVKNF ESRGVVLGILKGYVGLSGAIITQL+ 
Sbjct: 121 KPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYR 180

Query: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240
           AFYG DTK LIL+IGWLPA +SFAFLRTIRIMKV RQ NELKVFYNFLYISL LA FLM+
Sbjct: 181 AFYGEDTKELILMIGWLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMV 240

Query: 241 MIIIESKLMFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEK 300
           +III     FTQSEFGGSAAVV++LL LP+ VV++EE  LWK K  A+  P P+ ++TEK
Sbjct: 241 VIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEK 300

Query: 301 PNIPKIEHQKQTQQKQKQ-----KQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSA 360
           P +   E +    ++ K+     K PSCWTT+F+PPERG+D+TILQALFS+DMLILF + 
Sbjct: 301 PKLDSSEFKDDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLAT 360

Query: 361 ICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFP 420
           ICGVGGTLTAIDNLGQIG SLGYPKRS+STFV+LVSIWNY GRV SG VSEI L KYKFP
Sbjct: 361 ICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFP 420

Query: 421 RPLMLSLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYST 480
           RPLML++ LLLSC GHL+IAF+VP GLYVAS++IGFCFGAQWPLLFAIISE+FGLKYYST
Sbjct: 421 RPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYST 480

Query: 481 LYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIV 540
           LYNFGSVASPIG Y+LNV+VAG  YD EA KQ  A G  R  G++L C G  CFKLSFI+
Sbjct: 481 LYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFII 540

Query: 541 ITGVTLLGMLVSLILVIRTRSFYKSDIYKKFREEAEAEVAGNGAVVAA 584
           I  VTL G+LVS++LVIRT+ FYKSDIYKKFRE+A A      A  AA
Sbjct: 541 IAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFREKALAAEMEMAAPAAA 580

[Close]

BLAST of Spg010129 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 691.4 bits (1783), Expect = 6.2e-199
Identity = 349/551 (63.34%), Postives = 427/551 (77.50%), Query Frame = 0


Query: 23  VHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGV 82
           +H   GRWF+VFAS LIMA AGATY+F  YS DIKS LGYDQTTLNLL FFKDLGANVGV
Sbjct: 11  IHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGV 70

Query: 83  LSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMCLYICVGANSQSFAN 142
           LSGLI EVTP W VL+IG+ +NF GYFMIWL VT +++ PKVWQMCLYIC+GANSQ+FAN
Sbjct: 71  LSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFAN 130

Query: 143 TGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAIS 202
           TG+LVTCVKNF ESRGV+LG+LKGYVGLSGAI TQL+ A YG D+KSLILLI WLPAA+S
Sbjct: 131 TGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVS 190

Query: 203 FAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKLMFTQSEFGGSAAVV 262
             F+  IR  KV+RQ NEL VFY FLYIS+ LA FLM M I E ++ F+++ +  SA + 
Sbjct: 191 LVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATIC 250

Query: 263 LLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEKPNIPKIEHQKQTQQKQKQKQPS 322
             LLF+PL V V +E  +W +    I  P+ V++   K  +   + +      ++++  S
Sbjct: 251 CALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKS 310

Query: 323 CWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTLTAIDNLGQIGMSLGYPKRS 382
           C++T+FSPP RGED+TILQAL S DM+ILF +  CG+G +LTA+DNLGQIG SLGYP  +
Sbjct: 311 CFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHT 370

Query: 383 ISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLSCVGHLMIAFDVPNGL 442
           +S+FV+LVSIWNY GRV SGFVSE +L KYK PRPLM++L LLLSC GHL+IAF VP  +
Sbjct: 371 VSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSV 430

Query: 443 YVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDR 502
           Y+ASI++GF FGAQ PLLFAIISELFGLKYYSTL+N G +ASP+G Y+LNV+V G  YD+
Sbjct: 431 YIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDK 490

Query: 503 EAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKSDI 562
           EA KQL A+GL RK  ++L C G++C+KL F+++  VT  G LVSL L IRTR FYK DI
Sbjct: 491 EALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDI 550

Query: 563 YKKFREEAEAE 574
           YKKFRE  E+E
Sbjct: 551 YKKFRESPESE 561

[Close]

BLAST of Spg010129 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 3.2e-94
Identity = 216/583 (37.05%), Postives = 331/583 (56.78%), Query Frame = 0


Query: 16  SDIRTIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLNLLSFFKD 75
           S  R   V  I  RW V  A++ I + AG  Y+F   S  IKS L Y+Q  L+ L   KD
Sbjct: 3   STTREKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKD 62

Query: 76  LGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMCLYICVGA 135
           LG +VG ++G ++E+ P W  L +GAV N  GY  +WL VT R     +W MC+ I VG 
Sbjct: 63  LGDSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGN 122

Query: 136 NSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIG 195
           N +++ NTG+LV+ V+NF +SRG V+GILKG+ GL GAII+Q++   + S+  SLIL++ 
Sbjct: 123 NGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVA 182

Query: 196 WLPAAISFA---FLRTIRIMKVIRQPNELKVFYNFLY-ISLALAGFLMLMIIIESKLMFT 255
             PA +      F+R +   K IR  +     + F+Y + L LA +LM +++I+  ++ +
Sbjct: 183 VTPAVVVVCLMFFIRPVGGHKQIRPTDGAS--FTFIYGVCLLLAAYLMSVMLIQDLVVVS 242

Query: 256 QSEFGGSAAVVLLLLFLPLAVVV--------------IEEYNLWKLKTAAIGAPNPVQII 315
            +       V+ ++L +P+ V +              IEE  + K +    G   P  I+
Sbjct: 243 HNVITVFTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLIL 302

Query: 316 T----EKPN----IPKIEHQK-----QTQQKQKQKQPSCWTTIFSPPERGEDFTILQALF 375
           +    EKP     +P  E  K     Q Q  Q   + +        P RGEDFT+ QAL 
Sbjct: 303 SEVEDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALV 362

Query: 376 SMDMLILFFSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFV 435
             D  ++FFS + G G  LT IDNLGQ+  SLGY    +   V+++SIWN+LGR+  G+ 
Sbjct: 363 KADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYF 422

Query: 436 SEIVLTKYKFPRPLMLSLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAII 495
           SE+V+  Y +PRP+ +++  L+  VGH+  A+  P  +Y+ +++IG  +GA W ++ A  
Sbjct: 423 SELVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATA 482

Query: 496 SELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCF 555
           SELFGLK +  LYNF ++A+P G  V +  +A + YDREAE+Q  A G V    + L+C 
Sbjct: 483 SELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCN 542

Query: 556 GAECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKSDIYKKFR 568
           G+ CF L+ ++++G  ++  ++S+ILV RT+S Y + +Y K R
Sbjct: 543 GSICFFLTSLIMSGFCIIACMLSMILVRRTKSVY-THLYGKTR 578

[Close]

BLAST of Spg010129 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 3.3e-91
Identity = 200/560 (35.71%), Postives = 315/560 (56.25%), Query Frame = 0


Query: 21  IAVHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLNLLSFFKDLGANV 80
           +A  ++  +W  + AS+ I  +AG +Y F +YS  +KS   YDQ+TL+ +S FKD+G NV
Sbjct: 1   MATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNV 60

Query: 81  GVLSGLINEVTP--------------PWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQ 140
           GVLSGL+                   PWVV+ IGA+LNF GYF++W +VT  I  P V  
Sbjct: 61  GVLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPV 120

Query: 141 MCLYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSD 200
           MCL++ + A S +F NT ++V+ ++NF +  G  +GI+KG+VGLSGA++ QL+      D
Sbjct: 121 MCLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGD 180

Query: 201 TKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIES 260
            K+ ILL+  +P+ +S   +  +R+ K     +E K       +SL +A +LM+ II++S
Sbjct: 181 PKTFILLLAIVPSLLSVLVMPLVRVYKT-STVDEKKHLDGLSTLSLIIAAYLMITIILKS 240

Query: 261 KLMFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQI-ITEKPNIPK 320
            L         + AV+L+LL  PL V V    +  +   +++ +P    +  T    I  
Sbjct: 241 TLSLPSWANAVTLAVLLVLLSSPLLVAVRAHRDSIEKPLSSVYSPLVDNLEATTSGEILM 300

Query: 321 IEHQKQTQQKQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLILFFSAICGVGGTLTA 380
           ++  K                            +LQA+ ++D  +LF + ICG+G  ++ 
Sbjct: 301 LDEDK-------------------------SLNLLQAMCNVDFWLLFLAMICGMGSGIST 360

Query: 381 IDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLL 440
           I+N+ QIG SL Y    I++ + L +IWN++GR   G+VS+ +L +  +PRPL+++ TL 
Sbjct: 361 INNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLG 420

Query: 441 LSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASP 500
              +GHL+IA      LY  SI++G C+G+QW L+  I SELFG+K+  T+YN  S+ASP
Sbjct: 421 TMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASP 480

Query: 501 IGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGML 560
           +G Y+ +V++ G  YDR               GE   C+G  CF+L+++VI  V  LG L
Sbjct: 481 MGSYIFSVRLIGYIYDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLGFL 521

Query: 561 VSLILVIRTRSFYKSDIYKK 566
           VS +LV RT++ Y+  I++K
Sbjct: 541 VSCVLVFRTKTIYR-QIFEK 521

[Close]

BLAST of Spg010129 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 330.1 bits (845), Expect = 3.6e-90
Identity = 202/561 (36.01%), Postives = 314/561 (55.97%), Query Frame = 0


Query: 23  VHVITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGV 82
           + ++  +W  + AS+ I  A+GA+Y F +YS  +KS   YDQ+TL+ +S FKD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 83  LSGL----------------INEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQ 142
            SGL                I     PWVVL++GA+  F GYF+IW +VT  I  P V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 143 MCLYICVGANSQSFANTGSLVTCVKNFRESRGVVLGILKGYVGLSGAIITQLFHAFYGSD 202
           MCL++ + A SQ+F NT ++V+ V+NF +  G  +GI+KG++GLSGAI+ QL+      D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 203 TKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIES 262
             S ILL+   P  +S   +  +RI +     ++ K       +SL +A +LM++II+  
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYET-SVADDKKHLNGLSAVSLIIAAYLMIIIIL-- 240

Query: 263 KLMFTQSEFGGSAAVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPVQIITEKPNIPKI 322
           K  F  S +     +V LL+ L L +++           A     + ++        P I
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLI-----------ARRAQRDGMEKTVPHDYSPLI 300

Query: 323 EHQKQTQQKQKQKQPSCWTTIFSPPERG--EDFTILQALFSMDMLILFFSAICGVGGTLT 382
              K T    +  +        S  E G  E+  +LQA+  +   +LF + ICG+G  L+
Sbjct: 301 SSPKATTSGNQSSEGD------SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLS 360

Query: 383 AIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTL 442
            I+N+ QIG SL Y    I++ V+L SIWN+LGR  +G+ S+ +L K  +PRPL+++ TL
Sbjct: 361 TINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATL 420

Query: 443 LLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVAS 502
               +GHL+IA      LYV S+++G C+G+QW L+  I SELFG+++  T++N  SVAS
Sbjct: 421 GTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVAS 480

Query: 503 PIGLYVLNVKVAGNFYDREAEKQLIAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGM 562
           PIG Y+ +V++ G  YD+ A            +GE   C+G+ CF+LSFI++  V   G 
Sbjct: 481 PIGSYIFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFFGF 529

Query: 563 LVSLILVIRTRSFYKSDIYKK 566
           LV+++L  RT++ Y+  + K+
Sbjct: 541 LVAIVLFFRTKTLYRQILVKR 529

The following BLAST results are available for this feature:

BLAST of Spg010129 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573851.1	2.7e-297	92.28	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022944943.1	6.6e-296	92.11	protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita moschata]	[more]
XP_023541417.1	1.5e-292	90.91	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022956604.1	1.6e-289	90.00	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]
XP_023535761.1	2.7e-289	89.83	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg010129 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	5.9e-29	25.00	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
Q6CGU8	4.6e-05	24.89	Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28...	[more]

BLAST of Spg010129 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZF2	3.2e-296	92.11	protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326...	[more]
A0A6J1GZI8	7.7e-290	90.00	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1K186	9.4e-288	89.86	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A5D3CFW3	6.1e-287	89.60	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3BG09	6.1e-287	89.60	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 ...	[more]

BLAST of Spg010129 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39210.1	4.2e-240	72.11	Major facilitator superfamily protein	[more]
AT2G28120.1	6.2e-199	63.34	Major facilitator superfamily protein	[more]
AT5G14120.1	3.2e-94	37.05	Major facilitator superfamily protein	[more]
AT1G18940.1	3.3e-91	35.71	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G74780.1	3.6e-90	36.01	Nodulin-like / Major Facilitator Superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 29..274 e-value: 2.9E-95 score: 318.5
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 342..559 e-value: 5.0E-9 score: 37.8
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 28..556
None	No IPR available	PANTHER	PTHR21576:SF117	MFS TRANSPORTER	coord: 13..576
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 13..576
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 28..544 e-value: 2.06482E-111 score: 336.146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg010129.1	Spg010129.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0099587	inorganic ion import across plasma membrane
biological_process	GO:0002239	response to oomycetes
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0022857	transmembrane transporter activity