Spg010086 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg010086
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cucumisin-like
Location	scaffold7: 9344711 .. 9345771 (+)
RNA-Seq Expression	Spg010086
Synteny	Spg010086

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAAGCATCGGCGTGAAGGATTACGCCAGCTCTTATCCCTTGCCTGCTTCCTATCTCCTCATAACAGCCGCCAATGTCATTCAACTCTCTTCAACCGCGTACGTTTCAACGCACATTCTTTTTCAACTAAAAAAAAAAGAAAAACAACCCTCAAATAATGAAGCTATGTTTTTCAGATTATTCACTTAAAATTTTAATCTTTTGATCGACAGAGAATCGAAGACTGCAACCATTTTCAAGAGTATGGATGTGCAGGATGCTTCTACTCCTTTTGTAGTTTCCTTCTCCTCCAGGGGACCAAATCTTATAACCTTTGATATTCTCAAGGTTAAGCTCAGTTTGTTGCTTTTCAATTAGATGGATTTTTTGTGTTTTTATATTGTTTAGGTCTTGTTTTAAGATCGTTAAGTTTTCGTGCATGCAGCCGGATTTGACTGCACCAGGAGTTGAAATTCTAGCAGCGTGGTCTCCAATTGCACCGGTCTGCCTAGGAGATTCGAGGAGTGTGCTTTTTAATATAATCTCAGGAACGTCGATGTCTTGTCCACATGTCACTGCCATTGCTGCATACGTGAAAACATTCCATCCCACGTGGTTTCCTGCTGCCATAAAGTCGGCTCTCATGACAACCGGTAATAATGGATGATCCATGTAAAATTAGTGTAGAGCTATCTTTACACAAAAATCAACAAATAGTTACGTTAAATCACCAATAGATCATAATACAAATGTAACCTTTTCATTCGTACTCTTTAGAACTCATTTAATTACAAAAAGTTTTTCACTCACTTTCTGTTGATTCAATTTCTGCAGCTTTTACCATGAATGCCAAACTCAATCCACAAGCAGAGCTTGCATATGGTGCAGGCCGTCTCAACCCACTCATGGCAGTATATCCAGGGTTGGTCTACAATGCAAGTGTAAGCGACTACGCTAAATTCTTGTGTGGCCAAGGTTACACCATCGACATGATTCGGCATATTACTTGCAACAATAGTGCTCGGGCAACATTGGTGTTGGGAGAAAAATGCGGAATACAAATAAGAATAATATTATGA

mRNA sequence

ATGCAAAGCATCGGCGTGAAGGATTACGCCAGCTCTTATCCCTTGCCTGCTTCCTATCTCCTCATAACAGCCGCCAATGTCATTCAACTCTCTTCAACCGCAGAATCGAAGACTGCAACCATTTTCAAGAGTATGGATGTGCAGGATGCTTCTACTCCTTTTGTAGTTTCCTTCTCCTCCAGGGGACCAAATCTTATAACCTTTGATATTCTCAAGCCGGATTTGACTGCACCAGGAGTTGAAATTCTAGCAGCGTGGTCTCCAATTGCACCGGTCTGCCTAGGAGATTCGAGGAGTGTGCTTTTTAATATAATCTCAGGAACGTCGATGTCTTGTCCACATGTCACTGCCATTGCTGCATACGTGAAAACATTCCATCCCACGTGGTTTCCTGCTGCCATAAAGTCGGCTCTCATGACAACCGCTTTTACCATGAATGCCAAACTCAATCCACAAGCAGAGCTTGCATATGGTGCAGGCCGTCTCAACCCACTCATGGCAGTATATCCAGGGTTGGTCTACAATGCAAGTGTAAGCGACTACGCTAAATTCTTGTGTGGCCAAGGTTACACCATCGACATGATTCGGCATATTACTTGCAACAATAGTGCTCGGGCAACATTGGTGTTGGGAGAAAAATGCGGAATACAAATAAGAATAATATTATGA

Coding sequence (CDS)

ATGCAAAGCATCGGCGTGAAGGATTACGCCAGCTCTTATCCCTTGCCTGCTTCCTATCTCCTCATAACAGCCGCCAATGTCATTCAACTCTCTTCAACCGCAGAATCGAAGACTGCAACCATTTTCAAGAGTATGGATGTGCAGGATGCTTCTACTCCTTTTGTAGTTTCCTTCTCCTCCAGGGGACCAAATCTTATAACCTTTGATATTCTCAAGCCGGATTTGACTGCACCAGGAGTTGAAATTCTAGCAGCGTGGTCTCCAATTGCACCGGTCTGCCTAGGAGATTCGAGGAGTGTGCTTTTTAATATAATCTCAGGAACGTCGATGTCTTGTCCACATGTCACTGCCATTGCTGCATACGTGAAAACATTCCATCCCACGTGGTTTCCTGCTGCCATAAAGTCGGCTCTCATGACAACCGCTTTTACCATGAATGCCAAACTCAATCCACAAGCAGAGCTTGCATATGGTGCAGGCCGTCTCAACCCACTCATGGCAGTATATCCAGGGTTGGTCTACAATGCAAGTGTAAGCGACTACGCTAAATTCTTGTGTGGCCAAGGTTACACCATCGACATGATTCGGCATATTACTTGCAACAATAGTGCTCGGGCAACATTGGTGTTGGGAGAAAAATGCGGAATACAAATAAGAATAATATTATGA

Protein sequence

MQSIGVKDYASSYPLPASYLLITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSSRGPNLITFDILKPDLTAPGVEILAAWSPIAPVCLGDSRSVLFNIISGTSMSCPHVTAIAAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASVSDYAKFLCGQGYTIDMIRHITCNNSARATLVLGEKCGIQIRIIL

Homology

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BLAST of Spg010086 vs. NCBI nr
Match: XP_023548142.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 295.4 bits (755), Expect = 4.0e-76
Identity = 157/206 (76.21%), Postives = 171/206 (83.01%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSS 60
           MQ    KDYASSYPLPA+YL I AAN IQLSS  ES TATI +S  V+DAS P VVSFSS
Sbjct: 424 MQGTREKDYASSYPLPATYLHIAAANDIQLSSPTES-TATILQSNAVKDASAPSVVSFSS 483

Query: 61  RGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTAI 120
           RGPNL+T DILKPDLTAPGVEILAAWSPIAPV   +GDSRSV++NIISGTSMSCPH TA+
Sbjct: 484 RGPNLVTLDILKPDLTAPGVEILAAWSPIAPVSGVVGDSRSVVYNIISGTSMSCPHATAM 543

Query: 121 AAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASV 180
           A YVKTFHPTW PAAIKSALMTTAFTMN+KLN QAE AYGAG +NPL A+ PGLVYNAS 
Sbjct: 544 AVYVKTFHPTWSPAAIKSALMTTAFTMNSKLNTQAEFAYGAGHVNPLKAINPGLVYNASE 603

Query: 181 SDYAKFLCGQGYTIDMIRHITCNNSA 205
           SDY   LCGQGYT  M+R IT ++SA
Sbjct: 604 SDYIDMLCGQGYTTAMVRLITHDSSA 628

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BLAST of Spg010086 vs. NCBI nr
Match: KAG6600973.1 (hypothetical protein SDJN03_06206, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 293.1 bits (749), Expect = 2.0e-75
Identity = 156/206 (75.73%), Postives = 170/206 (82.52%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSS 60
           MQ    KDYASSYPLPA+YL I AAN IQLSS  ES TATI +S  V+DAS P VVSFSS
Sbjct: 283 MQGTRQKDYASSYPLPATYLHIAAANDIQLSSPTES-TATILQSNAVKDASAPSVVSFSS 342

Query: 61  RGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTAI 120
           RGPNL+T DILKPDLTAPGVEILAAWSPIAPV   +GDSRSV++NIISGTSMSCPH TA+
Sbjct: 343 RGPNLVTLDILKPDLTAPGVEILAAWSPIAPVSGVVGDSRSVVYNIISGTSMSCPHATAM 402

Query: 121 AAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASV 180
           A YVKTFHP W PAAIKSALMTTAFTMN+KLN QAE AYGAG +NPL A+ PGLVYNAS 
Sbjct: 403 AVYVKTFHPKWSPAAIKSALMTTAFTMNSKLNTQAEFAYGAGHVNPLKAINPGLVYNASE 462

Query: 181 SDYAKFLCGQGYTIDMIRHITCNNSA 205
           SDY   LCGQGYT  M+R IT ++SA
Sbjct: 463 SDYIDMLCGQGYTTAMVRLITHDSSA 487

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BLAST of Spg010086 vs. NCBI nr
Match: XP_022139190.1 (LOW QUALITY PROTEIN: cucumisin-like [Momordica charantia])

HSP 1 Score: 284.3 bits (726), Expect = 9.3e-73
Identity = 150/207 (72.46%), Postives = 167/207 (80.68%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQL-SSTAESKTATIFKSMDVQDASTPFVVSFS 60
           MQ + +KDYASSYP PASYL   A + IQL  S+    TATI KS+ VQDAS P VVSFS
Sbjct: 427 MQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFS 486

Query: 61  SRGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTA 120
           SRGP  IT DILK DL+APGVEILAAWSP+APV    GDSRS L+NIISGTSMSCPH TA
Sbjct: 487 SRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMSCPHATA 546

Query: 121 IAAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNAS 180
           IAAYVKTFHPTW PAA+KSALMTTAFTMNAKLNPQAE AYG+G +NPL AV PGLVY+A+
Sbjct: 547 IAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDAN 606

Query: 181 VSDYAKFLCGQGYTIDMIRHITCNNSA 205
             DY KFLCGQGY+  M+R IT ++SA
Sbjct: 607 EGDYVKFLCGQGYSTAMVRRITGDSSA 633

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BLAST of Spg010086 vs. NCBI nr
Match: XP_038891007.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 282.7 bits (722), Expect = 2.7e-72
Identity = 153/207 (73.91%), Postives = 167/207 (80.68%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSS 60
           MQ   +KDYASSYPLPASYL       I+LSS     TATIFKS    DAS P VVSFSS
Sbjct: 425 MQGSRLKDYASSYPLPASYL---HNEDIKLSS-----TATIFKSNANLDASAPSVVSFSS 484

Query: 61  RGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTAI 120
           RGPNL+T DILKPDLTAPGVEILAAWSPIAPV     D+RSVL+NIISGTSMSCPH TAI
Sbjct: 485 RGPNLVTLDILKPDLTAPGVEILAAWSPIAPVSGTARDTRSVLYNIISGTSMSCPHATAI 544

Query: 121 AAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASV 180
           A YVKTFHPTW PAAIKSALMTTAF+MN K+NPQAE AYGAG +NPL A+ PGLVYNAS 
Sbjct: 545 AVYVKTFHPTWSPAAIKSALMTTAFSMNGKVNPQAEFAYGAGHINPLKAINPGLVYNASE 604

Query: 181 SDYAKFLCG-QGYTIDMIRHITCNNSA 205
           +DY KFLCG +GYT DM+RHIT +N+A
Sbjct: 605 TDYIKFLCGHEGYTTDMVRHITGDNTA 623

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BLAST of Spg010086 vs. NCBI nr
Match: XP_031741167.1 (cucumisin [Cucumis sativus])

HSP 1 Score: 282.0 bits (720), Expect = 4.6e-72
Identity = 149/207 (71.98%), Postives = 166/207 (80.19%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSS 60
           MQ    KDYASSYPLPASYL  T  N +       S TATIFKS ++ +AS P VVSFSS
Sbjct: 409 MQGSRFKDYASSYPLPASYLHSTNINTL-------SSTATIFKSNEILNASAPSVVSFSS 468

Query: 61  RGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTAI 120
           RGPNL T DILKPDLTAPGVEILAAWSPIAPV    GDSRSVL+NIISGTSMSCPH TAI
Sbjct: 469 RGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGIAGDSRSVLYNIISGTSMSCPHATAI 528

Query: 121 AAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASV 180
           A YVKTF+PTW PAAIKSALMTTAF+MNAK+NP+AE AYGAG +NPL A+ PGLVYNA+ 
Sbjct: 529 AVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPEAEFAYGAGHINPLKALNPGLVYNATE 588

Query: 181 SDYAKFLCGQ-GYTIDMIRHITCNNSA 205
           +DY  FLCGQ GYT +M+RHIT + +A
Sbjct: 589 TDYINFLCGQEGYTTEMVRHITGDKTA 608

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BLAST of Spg010086 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.3e-61
Identity = 126/208 (60.58%), Postives = 150/208 (72.12%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYL----LITAANVIQLSSTAESKTATIFKSMDVQDASTPFVV 60
           + +   +DYA SYPLP+S L    L+     I    +  S  ATIFKS  + +AS P VV
Sbjct: 417 LMTSNTRDYADSYPLPSSVLDPNDLLATLRYIY---SIRSPGATIFKSTTILNASAPVVV 476

Query: 61  SFSSRGPNLITFDILKPDLTAPGVEILAAWSPIAPVCLGDSRSVLFNIISGTSMSCPHVT 120
           SFSSRGPN  T D++KPD++ PGVEILAAW  +APV  G  R+ LFNIISGTSMSCPH+T
Sbjct: 477 SFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVG-GIRRNTLFNIISGTSMSCPHIT 536

Query: 121 AIAAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNA 180
            IA YVKT++PTW PAAIKSALMTTA  MNA+ NPQAE AYG+G +NPL AV PGLVY+A
Sbjct: 537 GIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDA 596

Query: 181 SVSDYAKFLCGQGYTIDMIRHITCNNSA 205
           + SDY KFLCGQGY    +R IT + SA
Sbjct: 597 NESDYVKFLCGQGYNTQAVRRITGDYSA 620

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BLAST of Spg010086 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.3e-47
Identity = 101/203 (49.75%), Postives = 136/203 (67.00%), Query Frame = 0


Query: 7   KDYASSYPLPASYLLITAANVI--QLSSTAESKTATIFKSMDVQDASTPFVVSFSSRGPN 66
           +D AS +  P S L     N++   ++ST   K A + KS  + +   P V S+SSRGPN
Sbjct: 424 EDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPK-AAVLKSETIFNQKAPVVASYSSRGPN 483

Query: 67  LITFDILKPDLTAPGVEILAAWSPIAPVCLGDSRSVLFNIISGTSMSCPHVTAIAAYVKT 126
            +  DILKPD+TAPG EILAA+SP  P    D+R V + +ISGTSMSCPHV  +AAY+KT
Sbjct: 484 PLIHDILKPDITAPGSEILAAYSPYVPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKT 543

Query: 127 FHPTWFPAAIKSALMTTAFTMNAKLNPQ---AELAYGAGRLNPLMAVYPGLVYNASVSDY 186
           FHP W P+ I+SA+MTTA+ MNA  +P    AE AYGAG ++P+ A++PGLVY A+ SD+
Sbjct: 544 FHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDH 603

Query: 187 AKFLCGQGYTIDMIRHITCNNSA 205
             FLCG  YT   +R I+ ++S+
Sbjct: 604 ITFLCGFNYTGKKLRLISGDSSS 625

[Close]

BLAST of Spg010086 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.0e-45
Identity = 97/201 (48.26%), Postives = 136/201 (67.66%), Query Frame = 0


Query: 8   DYASSYPLPASYLLITAAN-VIQLSSTAESKTATIFKSMDVQDASTPFVVSFSSRGPNLI 67
           D AS +  P S LL    N V+   ++ ++  A + KS  + +   P V S+ SRGPN I
Sbjct: 425 DVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTI 484

Query: 68  TFDILKPDLTAPGVEILAAWSPIAPVCLGDSRSVLFNIISGTSMSCPHVTAIAAYVKTFH 127
             DILKPD+TAPG EI+AA+SP AP  + D+R V +++ +GTSMSCPHV  +AAY+K+FH
Sbjct: 485 IPDILKPDITAPGSEIVAAYSPDAPPSISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 544

Query: 128 PTWFPAAIKSALMTTAFTMNAKLNP---QAELAYGAGRLNPLMAVYPGLVYNASVSDYAK 187
           P W P+ I+SA+MTTA+ MNA  +P    AE AYGAG ++P+ A++PGLVY A+ SD+  
Sbjct: 545 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 604

Query: 188 FLCGQGYTIDMIRHITCNNSA 205
           FLCG  YT   +R I+ ++S+
Sbjct: 605 FLCGLNYTAKNLRLISGDSSS 625

[Close]

BLAST of Spg010086 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 7.2e-44
Identity = 96/211 (45.50%), Postives = 136/211 (64.45%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYL-LITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFS 60
           +Q+  + D A   P PAS L      ++     +AE   A I ++ ++ D   P+V SFS
Sbjct: 408 VQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFS 467

Query: 61  SRGPNLITFDILKPDLTAPGVEILAAWSPIAP----VCLGDSRSVLFNIISGTSMSCPHV 120
           SRGP+ +  ++LKPD++APG+EILAA+SP+A     +   D RSV ++++SGTSM+CPHV
Sbjct: 468 SRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHV 527

Query: 121 TAIAAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYN 180
             +AAYVK+FHP W P+AIKSA+MTTA  MN K NP+ E AYG+G++NP  A  PGLVY 
Sbjct: 528 AGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYE 587

Query: 181 ASVSDYAKFLCGQGY-----TIDMIRHITCN 202
               DY K LC +G+     T    +++TC+
Sbjct: 588 VETEDYLKMLCAEGFDSTTLTTTSGQNVTCS 618

[Close]

BLAST of Spg010086 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 6.1e-43
Identity = 89/182 (48.90%), Postives = 127/182 (69.78%), Query Frame = 0


Query: 26  NVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSSRGPNLITFDILKPDLTAPGVEILAA 85
           +++   ++ ++  AT+ KS ++ +   P V SFSSRGP+ I  DILKPD+TAPGVEILAA
Sbjct: 443 SLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAA 502

Query: 86  WSPIAPVCLG--DSRSVLFNIISGTSMSCPHVTAIAAYVKTFHPTWFPAAIKSALMTTAF 145
           +SP +       D+R V ++++SGTSM+CPHV  +AAYVKTFHP W P+ I+SA+MTTA+
Sbjct: 503 YSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAW 562

Query: 146 TMNAKLN--PQAELAYGAGRLNPLMAVYPGLVYNASVSDYAKFLCGQGYTIDMIRHITCN 204
            MNA  +     E AYG+G ++P+ A+ PGLVY  + +D+  FLCG  YT D +R I+ +
Sbjct: 563 PMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGD 622

[Close]

BLAST of Spg010086 vs. ExPASy TrEMBL
Match: A0A6J1CBL9 (LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010158 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 4.5e-73
Identity = 150/207 (72.46%), Postives = 167/207 (80.68%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQL-SSTAESKTATIFKSMDVQDASTPFVVSFS 60
           MQ + +KDYASSYP PASYL   A + IQL  S+    TATI KS+ VQDAS P VVSFS
Sbjct: 427 MQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFS 486

Query: 61  SRGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTA 120
           SRGP  IT DILK DL+APGVEILAAWSP+APV    GDSRS L+NIISGTSMSCPH TA
Sbjct: 487 SRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMSCPHATA 546

Query: 121 IAAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNAS 180
           IAAYVKTFHPTW PAA+KSALMTTAFTMNAKLNPQAE AYG+G +NPL AV PGLVY+A+
Sbjct: 547 IAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDAN 606

Query: 181 VSDYAKFLCGQGYTIDMIRHITCNNSA 205
             DY KFLCGQGY+  M+R IT ++SA
Sbjct: 607 EGDYVKFLCGQGYSTAMVRRITGDSSA 633

[Close]

BLAST of Spg010086 vs. ExPASy TrEMBL
Match: A0A0A0KS99 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187840 PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 2.2e-72
Identity = 149/207 (71.98%), Postives = 166/207 (80.19%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSS 60
           MQ    KDYASSYPLPASYL  T  N +       S TATIFKS ++ +AS P VVSFSS
Sbjct: 252 MQGSRFKDYASSYPLPASYLHSTNINTL-------SSTATIFKSNEILNASAPSVVSFSS 311

Query: 61  RGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTAI 120
           RGPNL T DILKPDLTAPGVEILAAWSPIAPV    GDSRSVL+NIISGTSMSCPH TAI
Sbjct: 312 RGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGIAGDSRSVLYNIISGTSMSCPHATAI 371

Query: 121 AAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASV 180
           A YVKTF+PTW PAAIKSALMTTAF+MNAK+NP+AE AYGAG +NPL A+ PGLVYNA+ 
Sbjct: 372 AVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPEAEFAYGAGHINPLKALNPGLVYNATE 431

Query: 181 SDYAKFLCGQ-GYTIDMIRHITCNNSA 205
           +DY  FLCGQ GYT +M+RHIT + +A
Sbjct: 432 TDYINFLCGQEGYTTEMVRHITGDKTA 451

[Close]

BLAST of Spg010086 vs. ExPASy TrEMBL
Match: A0A5A7TIC7 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold158G00080 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.1e-70
Identity = 148/207 (71.50%), Postives = 163/207 (78.74%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSS 60
           MQ    KDYASSYPLPASYL          ++   S TA+IFKS  + D+S P VVSFSS
Sbjct: 305 MQGSRFKDYASSYPLPASYL--------HNANITLSSTASIFKSNAILDSSAPSVVSFSS 364

Query: 61  RGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTAI 120
           RGPNL T DILKPDLTAPGVEILAAWSPIA V    GDSRSVL+NIISGTSMSCPH TAI
Sbjct: 365 RGPNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAI 424

Query: 121 AAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASV 180
           A YVKTF+PTW PAAIKSALMTTAF+MNAK+NPQAE AYGAG +NPL AV PGLVYNA+ 
Sbjct: 425 AVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATE 484

Query: 181 SDYAKFLCGQ-GYTIDMIRHITCNNSA 205
           +DY  FLCGQ GYT DM+R IT +N+A
Sbjct: 485 TDYINFLCGQEGYTTDMVRRITGDNTA 503

[Close]

BLAST of Spg010086 vs. ExPASy TrEMBL
Match: A0A1S3CF95 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.1e-70
Identity = 148/207 (71.50%), Postives = 163/207 (78.74%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSS 60
           MQ    KDYASSYPLPASYL          ++   S TA+IFKS  + D+S P VVSFSS
Sbjct: 428 MQGSRFKDYASSYPLPASYL--------HNANITLSSTASIFKSNAILDSSAPSVVSFSS 487

Query: 61  RGPNLITFDILKPDLTAPGVEILAAWSPIAPV--CLGDSRSVLFNIISGTSMSCPHVTAI 120
           RGPNL T DILKPDLTAPGVEILAAWSPIA V    GDSRSVL+NIISGTSMSCPH TAI
Sbjct: 488 RGPNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAI 547

Query: 121 AAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASV 180
           A YVKTF+PTW PAAIKSALMTTAF+MNAK+NPQAE AYGAG +NPL AV PGLVYNA+ 
Sbjct: 548 AVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATE 607

Query: 181 SDYAKFLCGQ-GYTIDMIRHITCNNSA 205
           +DY  FLCGQ GYT DM+R IT +N+A
Sbjct: 608 TDYINFLCGQEGYTTDMVRRITGDNTA 626

[Close]

BLAST of Spg010086 vs. ExPASy TrEMBL
Match: A0A6J1CEK6 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 8.7e-69
Identity = 143/206 (69.42%), Postives = 158/206 (76.70%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYLLITAANVIQ-LSSTAESKTATIFKSMDVQDASTPFVVSFS 60
           MQ  GVKD A SYPLPASYL   A   I+   ++  + TATI KS  V D S P VVSFS
Sbjct: 421 MQDAGVKDNARSYPLPASYLGTAAGGSIKNYMASNRAATATILKSNAVNDTSAPLVVSFS 480

Query: 61  SRGPNLITFDILKPDLTAPGVEILAAWSPIAPVC-LGDSRSVLFNIISGTSMSCPHVTAI 120
           SRGPN  TFDILKPDLTAPGVEILAAWSPIAP+  + DSR  L+NIISGTSMSCPH TA+
Sbjct: 481 SRGPNPETFDILKPDLTAPGVEILAAWSPIAPISGITDSRKSLYNIISGTSMSCPHATAV 540

Query: 121 AAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYNASV 180
           A YVKTFHPTW PAAIKSALMTTA  +N KLNPQAE AYGAG +NPL AV PGLVY+A  
Sbjct: 541 AVYVKTFHPTWSPAAIKSALMTTATPLNPKLNPQAEFAYGAGHVNPLKAVNPGLVYDAHE 600

Query: 181 SDYAKFLCGQGYTIDMIRHITCNNSA 205
           SDY KFLCGQGYT  M++ +T + SA
Sbjct: 601 SDYVKFLCGQGYTTAMVQRLTADASA 626

[Close]

BLAST of Spg010086 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 189.1 bits (479), Expect = 3.8e-48
Identity = 101/203 (49.75%), Postives = 136/203 (67.00%), Query Frame = 0


Query: 7   KDYASSYPLPASYLLITAANVI--QLSSTAESKTATIFKSMDVQDASTPFVVSFSSRGPN 66
           +D AS +  P S L     N++   ++ST   K A + KS  + +   P V S+SSRGPN
Sbjct: 424 EDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPK-AAVLKSETIFNQKAPVVASYSSRGPN 483

Query: 67  LITFDILKPDLTAPGVEILAAWSPIAPVCLGDSRSVLFNIISGTSMSCPHVTAIAAYVKT 126
            +  DILKPD+TAPG EILAA+SP  P    D+R V + +ISGTSMSCPHV  +AAY+KT
Sbjct: 484 PLIHDILKPDITAPGSEILAAYSPYVPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKT 543

Query: 127 FHPTWFPAAIKSALMTTAFTMNAKLNPQ---AELAYGAGRLNPLMAVYPGLVYNASVSDY 186
           FHP W P+ I+SA+MTTA+ MNA  +P    AE AYGAG ++P+ A++PGLVY A+ SD+
Sbjct: 544 FHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDH 603

Query: 187 AKFLCGQGYTIDMIRHITCNNSA 205
             FLCG  YT   +R I+ ++S+
Sbjct: 604 ITFLCGFNYTGKKLRLISGDSSS 625

[Close]

BLAST of Spg010086 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 184.9 bits (468), Expect = 7.1e-47
Identity = 97/201 (48.26%), Postives = 136/201 (67.66%), Query Frame = 0


Query: 8   DYASSYPLPASYLLITAAN-VIQLSSTAESKTATIFKSMDVQDASTPFVVSFSSRGPNLI 67
           D AS +  P S LL    N V+   ++ ++  A + KS  + +   P V S+ SRGPN I
Sbjct: 425 DVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTI 484

Query: 68  TFDILKPDLTAPGVEILAAWSPIAPVCLGDSRSVLFNIISGTSMSCPHVTAIAAYVKTFH 127
             DILKPD+TAPG EI+AA+SP AP  + D+R V +++ +GTSMSCPHV  +AAY+K+FH
Sbjct: 485 IPDILKPDITAPGSEIVAAYSPDAPPSISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 544

Query: 128 PTWFPAAIKSALMTTAFTMNAKLNP---QAELAYGAGRLNPLMAVYPGLVYNASVSDYAK 187
           P W P+ I+SA+MTTA+ MNA  +P    AE AYGAG ++P+ A++PGLVY A+ SD+  
Sbjct: 545 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 604

Query: 188 FLCGQGYTIDMIRHITCNNSA 205
           FLCG  YT   +R I+ ++S+
Sbjct: 605 FLCGLNYTAKNLRLISGDSSS 625

[Close]

BLAST of Spg010086 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 178.7 bits (452), Expect = 5.1e-45
Identity = 96/211 (45.50%), Postives = 136/211 (64.45%), Query Frame = 0


Query: 1   MQSIGVKDYASSYPLPASYL-LITAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFS 60
           +Q+  + D A   P PAS L      ++     +AE   A I ++ ++ D   P+V SFS
Sbjct: 372 VQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFS 431

Query: 61  SRGPNLITFDILKPDLTAPGVEILAAWSPIAP----VCLGDSRSVLFNIISGTSMSCPHV 120
           SRGP+ +  ++LKPD++APG+EILAA+SP+A     +   D RSV ++++SGTSM+CPHV
Sbjct: 432 SRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHV 491

Query: 121 TAIAAYVKTFHPTWFPAAIKSALMTTAFTMNAKLNPQAELAYGAGRLNPLMAVYPGLVYN 180
             +AAYVK+FHP W P+AIKSA+MTTA  MN K NP+ E AYG+G++NP  A  PGLVY 
Sbjct: 492 AGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYE 551

Query: 181 ASVSDYAKFLCGQGY-----TIDMIRHITCN 202
               DY K LC +G+     T    +++TC+
Sbjct: 552 VETEDYLKMLCAEGFDSTTLTTTSGQNVTCS 582

[Close]

BLAST of Spg010086 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 175.6 bits (444), Expect = 4.3e-44
Identity = 89/182 (48.90%), Postives = 127/182 (69.78%), Query Frame = 0


Query: 26  NVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSSRGPNLITFDILKPDLTAPGVEILAA 85
           +++   ++ ++  AT+ KS ++ +   P V SFSSRGP+ I  DILKPD+TAPGVEILAA
Sbjct: 443 SLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAA 502

Query: 86  WSPIAPVCLG--DSRSVLFNIISGTSMSCPHVTAIAAYVKTFHPTWFPAAIKSALMTTAF 145
           +SP +       D+R V ++++SGTSM+CPHV  +AAYVKTFHP W P+ I+SA+MTTA+
Sbjct: 503 YSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAW 562

Query: 146 TMNAKLN--PQAELAYGAGRLNPLMAVYPGLVYNASVSDYAKFLCGQGYTIDMIRHITCN 204
            MNA  +     E AYG+G ++P+ A+ PGLVY  + +D+  FLCG  YT D +R I+ +
Sbjct: 563 PMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGD 622

[Close]

BLAST of Spg010086 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 175.3 bits (443), Expect = 5.6e-44
Identity = 92/195 (47.18%), Postives = 129/195 (66.15%), Query Frame = 0


Query: 8   DYASSYPLPASYLLI-TAANVIQLSSTAESKTATIFKSMDVQDASTPFVVSFSSRGPNLI 67
           D A  +PLPA+ LL     +++    + +S  A + K+  + + ++P + SFSSRGPN I
Sbjct: 416 DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTI 475

Query: 68  TFDILKPDLTAPGVEILAAWSPIAPVCLGDSRSVLFNIISGTSMSCPHVTAIAAYVKTFH 127
             DILKPD+TAPGVEILAA+SP       D+R V ++++SGTSMSCPHV  +AAYVKTF+
Sbjct: 476 AVDILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFN 535

Query: 128 PTWFPAAIKSALMTTAFTMNAKLN--PQAELAYGAGRLNPLMAVYPGLVYNASVSDYAKF 187
           P W P+ I+SA+MTTA+ +NA        E AYG+G ++P+ A  PGLVY    SD+  F
Sbjct: 536 PKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAF 595

Query: 188 LCGQGYTIDMIRHIT 200
           LCG  YT  +++ I+
Sbjct: 596 LCGMNYTSQVLKVIS 610

The following BLAST results are available for this feature:

BLAST of Spg010086 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548142.1	4.0e-76	76.21	cucumisin-like [Cucurbita pepo subsp. pepo]	[more]
KAG6600973.1	2.0e-75	75.73	hypothetical protein SDJN03_06206, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022139190.1	9.3e-73	72.46	LOW QUALITY PROTEIN: cucumisin-like [Momordica charantia]	[more]
XP_038891007.1	2.7e-72	73.91	cucumisin-like [Benincasa hispida]	[more]
XP_031741167.1	4.6e-72	71.98	cucumisin [Cucumis sativus]	[more]

BLAST of Spg010086 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	1.3e-61	60.58	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9STF7	5.3e-47	49.75	Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S...	[more]
F4JA91	1.0e-45	48.26	Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S...	[more]
Q9FIF8	7.2e-44	45.50	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S...	[more]
Q9FGU3	6.1e-43	48.90	Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S...	[more]

BLAST of Spg010086 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CBL9	4.5e-73	72.46	LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC1110101...	[more]
A0A0A0KS99	2.2e-72	71.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187840 PE=3 SV=1	[more]
A0A5A7TIC7	2.1e-70	71.50	Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold158G00080...	[more]
A0A1S3CF95	2.1e-70	71.50	cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1	[more]
A0A6J1CEK6	8.7e-69	69.42	cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1	[more]

BLAST of Spg010086 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46850.1	3.8e-48	49.75	Subtilase family protein	[more]
AT3G46840.1	7.1e-47	48.26	Subtilase family protein	[more]
AT5G59190.1	5.1e-45	45.50	subtilase family protein	[more]
AT5G59100.1	4.3e-44	48.90	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59120.1	5.6e-44	47.18	subtilase 4.13	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.50.30.30		coord: 7..48 e-value: 8.8E-62 score: 211.3
None	No IPR available	PANTHER	PTHR10795:SF375	CUCUMISIN-LIKE	coord: 6..206
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 1..168 score: 12.748453
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 16..160 e-value: 2.1E-18 score: 66.6
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 49..181 e-value: 8.8E-62 score: 211.3
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 15..195
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 6..206
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 107..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg010086.1	Spg010086.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity