Spg010060 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg010060
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cysteine proteinase 15A
Location	scaffold7: 5762496 .. 5764836 (+)
RNA-Seq Expression	Spg010060
Synteny	Spg010060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAGGGCGCAATCCCTCATTCTAACTTGCGCATTCAGCCTCGCATTGCTAACCTGCGCACTGCCCTCCGCCGCCGCCCTCCGCCGAGGCCCGGAACTGCTGCGTCAAGTCACGGACGGCGACGACGACGGCGAGATCATCAACAGGCTGCCGCTGGGGAGCGAGAGGAAGTTCGTCACGTTCATGGAGAAGTACGGGAAGAGCTATCCGACGAGGAAGGAGTATCTGCACCGCCTCGGGATTTTTGCCAAAAATTTGATCAGGGCGGCGGAGCACCAGGCGCTCGATCCGACCGCCGTGCACGGCGTCACGCCGTTCTCGGACCTGTCGGAGGAGGAGTTCGAGCGGATGTTTATGGGCGTCAGAGGTGGAGCAGGCGGAGCGGAGGAGCTGCTGGAGAGGAATCGGGCGGCGGAGATGACGGCGGAGGAAGTGGAGGGGTTGCCGGAGAGGTTTGATTGGCGAGAGAAGGGGGCTGTTACCGGGGTTAAGATGCAGGTAAATTCGAGTCAAATTTCGGCCATTTACTGATTTGTCAGAGTTTCTTACTCGCATGCATGCTTTGAATATATTTTTTTTTACTGTGTGGATTTTGGAATTTTGTTACTAGAATTCGATGTTTTTTATTGGCTGATTTTTAACTATCTTGCAATGTTTTGATTGGTAGGTGTAGGTTAAATATATATTTTTATATTTGATTTAACTACTATTTCAGTTTTTTTTAGTTCAAAGAATATGTGGTTGGGAAATTCAAACTCATATAACCTTGGTCATAACTCACCATCACATACTTTGTCAATTGAGTTATGCTTTTGTTAGCATTGTTGTAGTTATTATACTTTAGTGTTTGGTTCATTTTGATCTCTATGTTTTAAAATGTCACTCTTTTTCATTTATGTTTATTTGTTTTGGTCCATATACTTTTGTTCAGAACATCTATTTTGATGTTTTTGTCCATATTCATTTTTTTCCTCTTATTTATTTCGATCCTAATTTTAGTACTACACCCAATAAATAGAATTTAGTGATAACTTGATATCAAATGATCTTGGTTGTATATAAACTTATGTTATAATTTAACAATTAGGAATTAAAATGGTCATTTTGAAATAGTACAATTACCAATTTTAACTTTTTTAAAGCATATAGAGAATAAAATGGACCAAAGTTGTAAGTATAAAAACTAACATAAATGTTTTGAGGATATAGAAGCAGATGACAAGTTTAAACTGTGTAAACATTGAAAGTTTCGTGTTAGATAGGTCCCTAAAAATTATCTCATTCTTTTATTTTTCTTTCATAAAATTATGTCAATGAACTTTAATACGTGTATCCAATCTTCAAATTAAAAACCCATGTAGTATATATATATTTTTTAATTCAACAATATTTGAGGTGAGATATTCGAACCTCAGACCTCTTGATCGAAGACACATGCCAATTACCGTTGAACTATGCTCGCTTTGGAAAAAACCTATATAGTATATTCTCACACTTTTAATTTTGTGTCTAATTCATTAAACTTTAAAAATGTCAACTAAGTCATCAAACTTTCAAATTTGTGTAGAGTCTTAATATATTCAACATGTGTTTAGAAAAAGTGAATCCAATTTATTAGATGTAGAATTGAAATTTGTGTCTAAATTCAGCACGTGTGGAGTAGTGGAAGGAGCCAACTTCATAGCCACCGGCAAGCTCCTCAGCCTCAGCGAACAACAGCTCGTCGATTGCGACCACACGGTCAGTACAGTACTCTCATTTCCAAATCCCCAATTTCAAAACCCTAATTCCAATTCCCAATTCCCAATTCCTTTCTCTTCTCCAGTGCGACGCCGCAGACAAGACCGCCTGCGACAACGGCTGCCACGGCGGCCTAATGACCAACGCCTACAAGTACCTGATCGACTCCGGCGGCCTCGAAGAAGAGGCCTCCTACCCCTACACCGGCCGCCGCGGCCAATGCAACTTCCAGTCCGACAAAATCGCCGTCAGGGTTTCCAATTTCACCACCATCCCCATCGACGAGGACCAGATCGCCGCCCACCTCGTCCGCCGCGGGCCGCTCGCCGTCGGCCTCAACGCCGTCTTCATGCAGACGTACATCGGCGGCGTCTCGTGTCCCCTGATCTGCGGCAAGAGATTCGTCAACCACGGCGTTCTTCTCGTCGGGTACGGCGACGAAGGGTTCTCGATTCTGCGGTTGCGGAAACTGCCGTACTGGATCATCAAGAACTCCTGGGGAGAAGGGTGGGGCGAGCGGGGGTATTATCGGCTGTGTCGCGGCCATGGCATGTGTGGAATGAATACTATGGTTTCGGCCGTCGTTACTCAGGCTTAG

mRNA sequence

ATGGCCAGGGCGCAATCCCTCATTCTAACTTGCGCATTCAGCCTCGCATTGCTAACCTGCGCACTGCCCTCCGCCGCCGCCCTCCGCCGAGGCCCGGAACTGCTGCGTCAAGTCACGGACGGCGACGACGACGGCGAGATCATCAACAGGCTGCCGCTGGGGAGCGAGAGGAAGTTCGTCACGTTCATGGAGAAGTACGGGAAGAGCTATCCGACGAGGAAGGAGTATCTGCACCGCCTCGGGATTTTTGCCAAAAATTTGATCAGGGCGGCGGAGCACCAGGCGCTCGATCCGACCGCCGTGCACGGCGTCACGCCGTTCTCGGACCTGTCGGAGGAGGAGTTCGAGCGGATGTTTATGGGCGTCAGAGGTGGAGCAGGCGGAGCGGAGGAGCTGCTGGAGAGGAATCGGGCGGCGGAGATGACGGCGGAGGAAGTGGAGGGGTTGCCGGAGAGGTTTGATTGGCGAGAGAAGGGGGCTGTTACCGGGGTTAAGATGCAGTGCGACGCCGCAGACAAGACCGCCTGCGACAACGGCTGCCACGGCGGCCTAATGACCAACGCCTACAAGTACCTGATCGACTCCGGCGGCCTCGAAGAAGAGGCCTCCTACCCCTACACCGGCCGCCGCGGCCAATGCAACTTCCAGTCCGACAAAATCGCCGTCAGGGTTTCCAATTTCACCACCATCCCCATCGACGAGGACCAGATCGCCGCCCACCTCGTCCGCCGCGGGCCGCTCGCCGTCGGCCTCAACGCCGTCTTCATGCAGACGTACATCGGCGGCGTCTCGTGTCCCCTGATCTGCGGCAAGAGATTCGTCAACCACGGCGTTCTTCTCGTCGGGTACGGCGACGAAGGGTTCTCGATTCTGCGGTTGCGGAAACTGCCGTACTGGATCATCAAGAACTCCTGGGGAGAAGGGTGGGGCGAGCGGGGGTATTATCGGCTGTGTCGCGGCCATGGCATGTGTGGAATGAATACTATGGTTTCGGCCGTCGTTACTCAGGCTTAG

Coding sequence (CDS)

ATGGCCAGGGCGCAATCCCTCATTCTAACTTGCGCATTCAGCCTCGCATTGCTAACCTGCGCACTGCCCTCCGCCGCCGCCCTCCGCCGAGGCCCGGAACTGCTGCGTCAAGTCACGGACGGCGACGACGACGGCGAGATCATCAACAGGCTGCCGCTGGGGAGCGAGAGGAAGTTCGTCACGTTCATGGAGAAGTACGGGAAGAGCTATCCGACGAGGAAGGAGTATCTGCACCGCCTCGGGATTTTTGCCAAAAATTTGATCAGGGCGGCGGAGCACCAGGCGCTCGATCCGACCGCCGTGCACGGCGTCACGCCGTTCTCGGACCTGTCGGAGGAGGAGTTCGAGCGGATGTTTATGGGCGTCAGAGGTGGAGCAGGCGGAGCGGAGGAGCTGCTGGAGAGGAATCGGGCGGCGGAGATGACGGCGGAGGAAGTGGAGGGGTTGCCGGAGAGGTTTGATTGGCGAGAGAAGGGGGCTGTTACCGGGGTTAAGATGCAGTGCGACGCCGCAGACAAGACCGCCTGCGACAACGGCTGCCACGGCGGCCTAATGACCAACGCCTACAAGTACCTGATCGACTCCGGCGGCCTCGAAGAAGAGGCCTCCTACCCCTACACCGGCCGCCGCGGCCAATGCAACTTCCAGTCCGACAAAATCGCCGTCAGGGTTTCCAATTTCACCACCATCCCCATCGACGAGGACCAGATCGCCGCCCACCTCGTCCGCCGCGGGCCGCTCGCCGTCGGCCTCAACGCCGTCTTCATGCAGACGTACATCGGCGGCGTCTCGTGTCCCCTGATCTGCGGCAAGAGATTCGTCAACCACGGCGTTCTTCTCGTCGGGTACGGCGACGAAGGGTTCTCGATTCTGCGGTTGCGGAAACTGCCGTACTGGATCATCAAGAACTCCTGGGGAGAAGGGTGGGGCGAGCGGGGGTATTATCGGCTGTGTCGCGGCCATGGCATGTGTGGAATGAATACTATGGTTTCGGCCGTCGTTACTCAGGCTTAG

Protein sequence

MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFMGVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQCDAADKTACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRGHGMCGMNTMVSAVVTQA

Homology

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BLAST of Spg010060 vs. NCBI nr
Match: XP_038892721.1 (probable cysteine protease RD19D [Benincasa hispida])

HSP 1 Score: 587.4 bits (1513), Expect = 7.8e-164
Identity = 299/377 (79.31%), Postives = 311/377 (82.49%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           MARA+SL+LTCAFSL LLTCA+ SA ALRR P LLRQVT    DGEIIN LP GSERKFV
Sbjct: 44  MARAESLLLTCAFSLTLLTCAIHSATALRRDPGLLRQVT----DGEIINNLPAGSERKFV 103

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVT FSDLSEEEFERMFM
Sbjct: 104 MFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFM 163

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVR G  GA EL E N+AAEM AEEVEGLPERFDWREKGAVTGVKMQ             
Sbjct: 164 GVRSGGAGA-ELREMNQAAEMMAEEVEGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCG 223

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 224 AVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 283

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           ++SYPYTGRRG C+FQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI
Sbjct: 284 DSSYPYTGRRGDCDFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 343

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVL+VGYGDEGFSILR RKLPYWIIKNSWGE WGERGYYRLCRG
Sbjct: 344 GGVSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRG 403

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BLAST of Spg010060 vs. NCBI nr
Match: XP_023535677.1 (probable cysteine protease RD19D [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.0 bits (1473), Expect = 3.4e-159
Identity = 295/377 (78.25%), Postives = 306/377 (81.17%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           M R+ SL+LTCAFSLALL C    A ALR+ PE LRQVT    DGEIIN LP GSERKFV
Sbjct: 1   MERSVSLLLTCAFSLALLAC----ATALRQSPEFLRQVT----DGEIINGLPAGSERKFV 60

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHRLGIFAKNL+RAAEHQALDPTAVHGVT F+DLSEEEFE+MFM
Sbjct: 61  MFMEKYGKSYPTRKEYLHRLGIFAKNLVRAAEHQALDPTAVHGVTQFADLSEEEFEQMFM 120

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGGAGGA ELLE N+A EMTA   EGLPERFDWREKGAVT VKMQ             
Sbjct: 121 GVRGGAGGA-ELLEMNQAEEMTA---EGLPERFDWREKGAVTAVKMQGTCGSCWAFSTCG 180

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 181 AVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 240

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTGRRG+CNFQSDKIAVRVSNFTTIPIDEDQIAAHLV RGPLAVGLNAVFMQTYI
Sbjct: 241 ESSYPYTGRRGECNFQSDKIAVRVSNFTTIPIDEDQIAAHLVHRGPLAVGLNAVFMQTYI 300

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVLLVGYGDEGFSILR RKLPYWIIKNSWGE WGERGYYRLCRG
Sbjct: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRG 360

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BLAST of Spg010060 vs. NCBI nr
Match: XP_022956816.1 (probable cysteine protease RD19D [Cucurbita moschata])

HSP 1 Score: 571.6 bits (1472), Expect = 4.4e-159
Identity = 295/377 (78.25%), Postives = 305/377 (80.90%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           M R+ SL+LTCAFSLALL C    A ALR+GPE LRQVTDG     IIN LP GSERKFV
Sbjct: 1   MERSVSLLLTCAFSLALLAC----ATALRQGPEFLRQVTDGG----IINSLPAGSERKFV 60

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHRLGIFAKNL+RAAEHQALDPTAVHGVT FSDLSEEEFE+MFM
Sbjct: 61  MFMEKYGKSYPTRKEYLHRLGIFAKNLVRAAEHQALDPTAVHGVTQFSDLSEEEFEQMFM 120

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGGAGGA ELLE N+A EMTA   EGLPERFDWREKGAVT VKMQ             
Sbjct: 121 GVRGGAGGA-ELLEMNQAEEMTA---EGLPERFDWREKGAVTAVKMQGTCGSCWAFSTCG 180

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 181 AVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 240

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTG RG+CNFQSDKIAVRVSNFTTIPIDEDQIAAHLV RGPLAVGLNAVFMQTYI
Sbjct: 241 ESSYPYTGHRGECNFQSDKIAVRVSNFTTIPIDEDQIAAHLVHRGPLAVGLNAVFMQTYI 300

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVLLVGYGDEGFSILR RKLPYWIIKNSWGE WGERGYYRLCRG
Sbjct: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRG 360

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BLAST of Spg010060 vs. NCBI nr
Match: KAG7031982.1 (putative cysteine protease RD19D, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 571.2 bits (1471), Expect = 5.8e-159
Identity = 295/377 (78.25%), Postives = 305/377 (80.90%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           M R+ SL+LTCAFSLALL C    A ALR+ PE LRQVT    DGEIIN LP GSERKFV
Sbjct: 1   MERSVSLLLTCAFSLALLDC----ATALRQSPEFLRQVT----DGEIINSLPAGSERKFV 60

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHRLGIFAKNL+RAAEHQALDPTAVHGVT FSDLSEEEFE+MFM
Sbjct: 61  MFMEKYGKSYPTRKEYLHRLGIFAKNLVRAAEHQALDPTAVHGVTQFSDLSEEEFEQMFM 120

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGGAGGA ELLE N+A EMTA   EGLPERFDWREKGAVT VKMQ             
Sbjct: 121 GVRGGAGGA-ELLEMNQAEEMTA---EGLPERFDWREKGAVTAVKMQGTCGSCWAFSTCG 180

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 181 AVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 240

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTG RG+CNFQSDKIAVRVSNFTTIPIDEDQIAAHLV RGPLAVGLNAVFMQTYI
Sbjct: 241 ESSYPYTGHRGECNFQSDKIAVRVSNFTTIPIDEDQIAAHLVHRGPLAVGLNAVFMQTYI 300

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVLLVGYGDEGFSILR RKLPYWIIKNSWGE WGERGYYRLCRG
Sbjct: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRG 360

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BLAST of Spg010060 vs. NCBI nr
Match: XP_004142497.3 (probable cysteine protease RD19D [Cucumis sativus] >KAE8648852.1 hypothetical protein Csa_007817 [Cucumis sativus])

HSP 1 Score: 566.6 bits (1459), Expect = 1.4e-157
Identity = 288/377 (76.39%), Postives = 303/377 (80.37%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           MA A +L+L CA SLALL  A+PSA ALRR PE LRQVT    DGEI N LP GSERKFV
Sbjct: 38  MATAVTLLLACAISLALLISAIPSATALRRDPEFLRQVT----DGEIFNNLPAGSERKFV 97

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHR GIF KNLIRAAEHQALDPTAVHGVT FSDLSEEEFERMFM
Sbjct: 98  MFMEKYGKSYPTRKEYLHRFGIFVKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFM 157

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGGAGG E L E N+A E+TAEEV+GLPERFDWR+KGAVT VKMQ             
Sbjct: 158 GVRGGAGG-EGLPEMNQAVEVTAEEVKGLPERFDWRDKGAVTEVKMQGTCGSCWAFSTCG 217

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 218 AVEGANFIATGNLLNLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 277

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTGR GQCNFQSDKIAV+VSNFTTIPIDE+QIAAHLVR GPLAVGLNAVFMQTYI
Sbjct: 278 ESSYPYTGRSGQCNFQSDKIAVKVSNFTTIPIDENQIAAHLVRSGPLAVGLNAVFMQTYI 337

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVL+VGYGDEGFSILR RKLPYW+IKNSWGE WGE GYYRLCRG
Sbjct: 338 GGVSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWVIKNSWGERWGEHGYYRLCRG 397

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BLAST of Spg010060 vs. ExPASy Swiss-Prot
Match: Q8VYS0 (Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.2e-113
Identity = 216/367 (58.86%), Postives = 251/367 (68.39%), Query Frame = 0


Query: 17  LLTCALPSAAALRRGPEL-LRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRKE 76
           L+TC +     +    +L +RQVT  D+     N L   +E KF  FM  YGK+Y TR+E
Sbjct: 9   LITCIILFCHVVASVEDLTIRQVT-ADNRRIRPNLLGTHTESKFRLFMSDYGKNYSTREE 68

Query: 77  YLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFMGV------RGGAGGA 136
           Y+HRLGIFAKN+++AAEHQ +DP+AVHGVT FSDL+EEEF+RM+ GV      RGG  GA
Sbjct: 69  YIHRLGIFAKNVLKAAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGA 128

Query: 137 EELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ---------------------- 196
           E        A M   EV+GLPE FDWREKG VT VK Q                      
Sbjct: 129 E--------APMV--EVDGLPEDFDWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVS 188

Query: 197 ------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGR 256
                             CD  DK ACDNGC GGLMTNAY+YL+++GGLEEE SYPYTG+
Sbjct: 189 TGKLLSLSEQQLVDCDQACDPKDKKACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTGK 248

Query: 257 RGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLIC 316
           RG C F  +K+AVRV NFTTIP+DE+QIAA+LVR GPLAVGLNAVFMQTYIGGVSCPLIC
Sbjct: 249 RGHCKFDPEKVAVRVLNFTTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLIC 308

Query: 317 GKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRGHGMCGMNTM 337
            KR VNHGVLLVGYG +GFSILRL   PYWIIKNSWG+ WGE GYY+LCRGH +CG+N+M
Sbjct: 309 SKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSM 364

[Close]

BLAST of Spg010060 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.7e-85
Identity = 165/362 (45.58%), Postives = 216/362 (59.67%), Query Frame = 0


Query: 15  LALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRK 74
           L  L  A   AAA+     L+RQV  G DD    N L L +E  F++F++++GKSY    
Sbjct: 8   LLSLASAAAVAAAVDAEDPLIRQVVPGGDD----NDLELNAESHFLSFVQRFGKSYKDAD 67

Query: 75  EYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFMGVRGGAGGAEELLE 134
           E+ +RL +F  NL RA  HQ LDP+A HGVT FSDL+  EF R ++G+R         L 
Sbjct: 68  EHAYRLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRKSRRALLRELG 127

Query: 135 RNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ--------------------------- 194
            + A E      +GLP+ FDWR+ GAV  VK Q                           
Sbjct: 128 ES-AHEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEGAHYLATGKLE 187

Query: 195 -------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGRRGQCN 254
                        CD+++  +CD+GC+GGLMT A+ YL  +GGLE E  YPYTG  G+C 
Sbjct: 188 VLSEQQFVDCDHECDSSEPDSCDSGCNGGLMTTAFSYLQKAGGLESEKDYPYTGSDGKCK 247

Query: 255 FQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFV 314
           F   KI   V NF+ + +DE QI+A+L++ GPLA+G+NA +MQTYIGGVSCP ICG R +
Sbjct: 248 FDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSCPYICG-RHL 307

Query: 315 NHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRGHGM---CGMNTMVS 334
           +HGVLLVGYG  GF+ +RL+  PYWIIKNSWGE WGE GYY++CRG  +   CG+++MVS
Sbjct: 308 DHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNKCGVDSMVS 363

[Close]

BLAST of Spg010060 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.1e-83
Identity = 151/340 (44.41%), Postives = 206/340 (60.59%), Query Frame = 0


Query: 34  LLRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEH 93
           ++RQV D ++D        L +E  F +F  K+ KSY T++E+ +R G+F  NLI+A  H
Sbjct: 29  IIRQVVDNEEDH------LLNAEHHFTSFKSKFSKSYATKEEHDYRFGVFKSNLIKAKLH 88

Query: 94  QALDPTAVHGVTPFSDLSEEEFERMFMGVRGGAGGAEELLERNRAAEMTAEEVEGLPERF 153
           Q  DPTA HG+T FSDL+  EF R F+G++      + L     A +        LPE F
Sbjct: 89  QNRDPTAEHGITKFSDLTASEFRRQFLGLK------KRLRLPAHAQKAPILPTTNLPEDF 148

Query: 154 DWREKGAVTGVKMQ----------------------------------------CDAADK 213
           DWREKGAVT VK Q                                        CD    
Sbjct: 149 DWREKGAVTPVKDQGSCGSCWAFSTTGALEGAHYLATGKLVSLSEQQLVDCDHVCDPEQA 208

Query: 214 TACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGRRGQCNFQSDKIAVRVSNFTTIPID 273
            +CD+GC+GGLM NA++YL++SGG+ +E  Y YTGR G C F   K+   VSNF+ + +D
Sbjct: 209 GSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYTGRDGSCKFDKSKVVASVSNFSVVTLD 268

Query: 274 EDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLLVGYGDEGFSILRL 333
           EDQIAA+LV+ GPLAV +NA +MQTY+ GVSCP +C K  ++HGVLLVG+G   ++ +RL
Sbjct: 269 EDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCAKSRLDHGVLLVGFGKGAYAPIRL 328

[Close]

BLAST of Spg010060 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 300.1 bits (767), Expect = 3.2e-80
Identity = 152/341 (44.57%), Postives = 204/341 (59.82%), Query Frame = 0


Query: 34  LLRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEH 93
           L+RQV D  +         L SE  F  F +K+GK Y + +E+ +R  +F  NL+RA  H
Sbjct: 29  LIRQVVDETEP------KVLSSEDHFTLFKKKFGKVYGSIEEHYYRFSVFKANLLRAMRH 88

Query: 94  QALDPTAVHGVTPFSDLSEEEFERMFMGVRGGAGGAEELLERNRAAEMTAEEVEGLPERF 153
           Q +DP+A HGVT FSDL+  EF R  +GV+GG    ++      A +      + LPE F
Sbjct: 89  QKMDPSARHGVTQFSDLTRSEFRRKHLGVKGGFKLPKD------ANQAPILPTQNLPEEF 148

Query: 154 DWREKGAVTGVKMQ----------------------------------------CDAADK 213
           DWR++GAVT VK Q                                        CD  ++
Sbjct: 149 DWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHECDPEEE 208

Query: 214 TACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGR-RGQCNFQSDKIAVRVSNFTTIPI 273
            +CD+GC+GGLM +A++Y + +GGL  E  YPYTG   G C     KI   VSNF+ + I
Sbjct: 209 GSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCKLDRSKIVASVSNFSVVSI 268

Query: 274 DEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLLVGYGDEGFSILR 333
           +EDQIAA+L++ GPLAV +NA +MQTYIGGVSCP IC +R +NHGVLLVGYG  GFS  R
Sbjct: 269 NEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRR-LNHGVLLVGYGSAGFSQAR 328

[Close]

BLAST of Spg010060 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-79
Identity = 156/341 (45.75%), Postives = 204/341 (59.82%), Query Frame = 0


Query: 35  LRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQ 94
           +RQV   ++D +++N     +E  F  F  KY K+Y T+ E+ HR  +F  NL RA  +Q
Sbjct: 36  IRQVVPEENDEQLLN-----AEHHFTLFKSKYEKTYATQVEHDHRFRVFKANLRRARRNQ 95

Query: 95  ALDPTAVHGVTPFSDLSEEEFERMFMGVRGGAGGAEELLERNRAAEMTAEEVEGLPERFD 154
            LDP+AVHGVT FSDL+ +EF R F+G++    G     +   A  +   +   LP  FD
Sbjct: 96  LLDPSAVHGVTQFSDLTPKEFRRKFLGLK--RRGFRLPTDTQTAPILPTSD---LPTEFD 155

Query: 155 WREKGAVTGVKMQ----------------------------------------CDAADKT 214
           WRE+GAVT VK Q                                        CD A   
Sbjct: 156 WREQGAVTPVKNQGMCGSCWSFSAIGALEGAHFLATKELVSLSEQQLVDCDHECDPAQAN 215

Query: 215 ACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGR-RGQCNFQSDKIAVRVSNFTTIPID 274
           +CD+GC GGLM NA++Y + +GGL +E  YPYTGR    C F   KI   VSNF+ +  D
Sbjct: 216 SCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYTGRDHTACKFDKSKIVASVSNFSVVSSD 275

Query: 275 EDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLLVGYGDEGFSILRL 334
           EDQIAA+LV+ GPLA+ +NA++MQTYIGGVSCP +C K   +HGVLLVG+G  G++ +RL
Sbjct: 276 EDQIAANLVQHGPLAIAINAMWMQTYIGGVSCPYVCSKS-QDHGVLLVGFGSSGYAPIRL 335

[Close]

BLAST of Spg010060 vs. ExPASy TrEMBL
Match: A0A6J1GXJ8 (probable cysteine protease RD19D OS=Cucurbita moschata OX=3662 GN=LOC111458402 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.1e-159
Identity = 295/377 (78.25%), Postives = 305/377 (80.90%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           M R+ SL+LTCAFSLALL C    A ALR+GPE LRQVTDG     IIN LP GSERKFV
Sbjct: 1   MERSVSLLLTCAFSLALLAC----ATALRQGPEFLRQVTDGG----IINSLPAGSERKFV 60

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHRLGIFAKNL+RAAEHQALDPTAVHGVT FSDLSEEEFE+MFM
Sbjct: 61  MFMEKYGKSYPTRKEYLHRLGIFAKNLVRAAEHQALDPTAVHGVTQFSDLSEEEFEQMFM 120

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGGAGGA ELLE N+A EMTA   EGLPERFDWREKGAVT VKMQ             
Sbjct: 121 GVRGGAGGA-ELLEMNQAEEMTA---EGLPERFDWREKGAVTAVKMQGTCGSCWAFSTCG 180

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 181 AVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 240

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTG RG+CNFQSDKIAVRVSNFTTIPIDEDQIAAHLV RGPLAVGLNAVFMQTYI
Sbjct: 241 ESSYPYTGHRGECNFQSDKIAVRVSNFTTIPIDEDQIAAHLVHRGPLAVGLNAVFMQTYI 300

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVLLVGYGDEGFSILR RKLPYWIIKNSWGE WGERGYYRLCRG
Sbjct: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRFRKLPYWIIKNSWGERWGERGYYRLCRG 360

[Close]

BLAST of Spg010060 vs. ExPASy TrEMBL
Match: A0A0A0KTI9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622590 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.9e-158
Identity = 288/377 (76.39%), Postives = 303/377 (80.37%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           MA A +L+L CA SLALL  A+PSA ALRR PE LRQVT    DGEI N LP GSERKFV
Sbjct: 35  MATAVTLLLACAISLALLISAIPSATALRRDPEFLRQVT----DGEIFNNLPAGSERKFV 94

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHR GIF KNLIRAAEHQALDPTAVHGVT FSDLSEEEFERMFM
Sbjct: 95  MFMEKYGKSYPTRKEYLHRFGIFVKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFM 154

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGGAGG E L E N+A E+TAEEV+GLPERFDWR+KGAVT VKMQ             
Sbjct: 155 GVRGGAGG-EGLPEMNQAVEVTAEEVKGLPERFDWRDKGAVTEVKMQGTCGSCWAFSTCG 214

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 215 AVEGANFIATGNLLNLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 274

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTGR GQCNFQSDKIAV+VSNFTTIPIDE+QIAAHLVR GPLAVGLNAVFMQTYI
Sbjct: 275 ESSYPYTGRSGQCNFQSDKIAVKVSNFTTIPIDENQIAAHLVRSGPLAVGLNAVFMQTYI 334

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVL+VGYGDEGFSILR RKLPYW+IKNSWGE WGE GYYRLCRG
Sbjct: 335 GGVSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWVIKNSWGERWGEHGYYRLCRG 394

[Close]

BLAST of Spg010060 vs. ExPASy TrEMBL
Match: A0A6J1JBC1 (probable cysteine protease RD19D OS=Cucurbita maxima OX=3661 GN=LOC111482910 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.4e-157
Identity = 290/377 (76.92%), Postives = 302/377 (80.11%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           M R+ SL+LTCAFSL LL C    A ALR+GPE LRQ+T    DGEIIN LP GSERKFV
Sbjct: 1   MERSVSLLLTCAFSLTLLAC----ATALRQGPEFLRQIT----DGEIINSLPAGSERKFV 60

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHRLGIFAKNL+RAAEHQALDPTA+HGVT FSDLSEEEFE MFM
Sbjct: 61  MFMEKYGKSYPTRKEYLHRLGIFAKNLVRAAEHQALDPTAMHGVTQFSDLSEEEFELMFM 120

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGG GGA ELLE N+A EMTA   EGLPERFDWREKGAVT VKMQ             
Sbjct: 121 GVRGGVGGA-ELLEMNQAEEMTA---EGLPERFDWREKGAVTAVKMQGTCGSCWAFSTCG 180

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 181 AVEGANFIATGKLLSLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 240

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTG RG+CNFQS KIAVRVSNFTTIPIDEDQIAAHLV RGPLAVGLNAVFMQTYI
Sbjct: 241 ESSYPYTGHRGECNFQSHKIAVRVSNFTTIPIDEDQIAAHLVHRGPLAVGLNAVFMQTYI 300

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVLLVGYGDEGFSILR RKLPYWIIKNSWGE WGE+GYYRLCRG
Sbjct: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRFRKLPYWIIKNSWGERWGEQGYYRLCRG 360

[Close]

BLAST of Spg010060 vs. ExPASy TrEMBL
Match: A0A5D3CBQ6 (Cysteine proteinase 15A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002450 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.8e-157
Identity = 289/377 (76.66%), Postives = 302/377 (80.11%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           MA A +L L CAFSL LL  A+PSA ALR  PE LRQVT    DGEI N LP GSERKF 
Sbjct: 1   MATAVTL-LACAFSLTLLISAIPSATALRHDPEFLRQVT----DGEIFNNLPAGSERKFA 60

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHRLGIF KNLIRAAEHQALDPTAVHGVT FSDLSEEEFERMFM
Sbjct: 61  MFMEKYGKSYPTRKEYLHRLGIFVKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFM 120

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGGAGGA  L E N+A E++AEEV+GLPERFDWREKGAVTGVKMQ             
Sbjct: 121 GVRGGAGGA-GLPEMNQAVEVSAEEVKGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCG 180

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 181 AVEGANFIATGKLLNLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 240

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTGR GQCNFQSDKIAV+VSNFTTIPIDE+QIAAHLVR GPLAVGLNAVFMQTYI
Sbjct: 241 ESSYPYTGRSGQCNFQSDKIAVKVSNFTTIPIDENQIAAHLVRSGPLAVGLNAVFMQTYI 300

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVL+VGYGDEGFSILR RKLPYWIIKNSWGE WGE GYYRLCRG
Sbjct: 301 GGVSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGEHGYYRLCRG 360

[Close]

BLAST of Spg010060 vs. ExPASy TrEMBL
Match: A0A1S3BG12 (cysteine proteinase 15A OS=Cucumis melo OX=3656 GN=LOC103489441 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.8e-157
Identity = 289/377 (76.66%), Postives = 302/377 (80.11%), Query Frame = 0


Query: 1   MARAQSLILTCAFSLALLTCALPSAAALRRGPELLRQVTDGDDDGEIINRLPLGSERKFV 60
           MA A +L L CAFSL LL  A+PSA ALR  PE LRQVT    DGEI N LP GSERKF 
Sbjct: 1   MATAVTL-LACAFSLTLLISAIPSATALRHDPEFLRQVT----DGEIFNNLPAGSERKFA 60

Query: 61  TFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFM 120
            FMEKYGKSYPTRKEYLHRLGIF KNLIRAAEHQALDPTAVHGVT FSDLSEEEFERMFM
Sbjct: 61  MFMEKYGKSYPTRKEYLHRLGIFVKNLIRAAEHQALDPTAVHGVTQFSDLSEEEFERMFM 120

Query: 121 GVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ------------- 180
           GVRGGAGGA  L E N+A E++AEEV+GLPERFDWREKGAVTGVKMQ             
Sbjct: 121 GVRGGAGGA-GLPEMNQAVEVSAEEVKGLPERFDWREKGAVTGVKMQGTCGSCWAFSTCG 180

Query: 181 ---------------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEE 240
                                      CD  DKTAC+NGC+GGLMTNAYKYLI SGGLEE
Sbjct: 181 AVEGANFIATGKLLNLSEQQLVDCDHTCDPTDKTACNNGCNGGLMTNAYKYLIQSGGLEE 240

Query: 241 EASYPYTGRRGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYI 300
           E+SYPYTGR GQCNFQSDKIAV+VSNFTTIPIDE+QIAAHLVR GPLAVGLNAVFMQTYI
Sbjct: 241 ESSYPYTGRSGQCNFQSDKIAVKVSNFTTIPIDENQIAAHLVRSGPLAVGLNAVFMQTYI 300

Query: 301 GGVSCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG 338
           GGVSCPLICGKRFVNHGVL+VGYGDEGFSILR RKLPYWIIKNSWGE WGE GYYRLCRG
Sbjct: 301 GGVSCPLICGKRFVNHGVLMVGYGDEGFSILRFRKLPYWIIKNSWGERWGEHGYYRLCRG 360

[Close]

BLAST of Spg010060 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 410.2 bits (1053), Expect = 1.6e-114
Identity = 216/367 (58.86%), Postives = 251/367 (68.39%), Query Frame = 0


Query: 17  LLTCALPSAAALRRGPEL-LRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRKE 76
           L+TC +     +    +L +RQVT  D+     N L   +E KF  FM  YGK+Y TR+E
Sbjct: 9   LITCIILFCHVVASVEDLTIRQVT-ADNRRIRPNLLGTHTESKFRLFMSDYGKNYSTREE 68

Query: 77  YLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERMFMGV------RGGAGGA 136
           Y+HRLGIFAKN+++AAEHQ +DP+AVHGVT FSDL+EEEF+RM+ GV      RGG  GA
Sbjct: 69  YIHRLGIFAKNVLKAAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGA 128

Query: 137 EELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ---------------------- 196
           E        A M   EV+GLPE FDWREKG VT VK Q                      
Sbjct: 129 E--------APMV--EVDGLPEDFDWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVS 188

Query: 197 ------------------CDAADKTACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGR 256
                             CD  DK ACDNGC GGLMTNAY+YL+++GGLEEE SYPYTG+
Sbjct: 189 TGKLLSLSEQQLVDCDQACDPKDKKACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTGK 248

Query: 257 RGQCNFQSDKIAVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLIC 316
           RG C F  +K+AVRV NFTTIP+DE+QIAA+LVR GPLAVGLNAVFMQTYIGGVSCPLIC
Sbjct: 249 RGHCKFDPEKVAVRVLNFTTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLIC 308

Query: 317 GKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRGHGMCGMNTM 337
            KR VNHGVLLVGYG +GFSILRL   PYWIIKNSWG+ WGE GYY+LCRGH +CG+N+M
Sbjct: 309 SKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSM 364

[Close]

BLAST of Spg010060 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 300.1 bits (767), Expect = 2.3e-81
Identity = 152/341 (44.57%), Postives = 204/341 (59.82%), Query Frame = 0


Query: 34  LLRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEH 93
           L+RQV D  +         L SE  F  F +K+GK Y + +E+ +R  +F  NL+RA  H
Sbjct: 29  LIRQVVDETEP------KVLSSEDHFTLFKKKFGKVYGSIEEHYYRFSVFKANLLRAMRH 88

Query: 94  QALDPTAVHGVTPFSDLSEEEFERMFMGVRGGAGGAEELLERNRAAEMTAEEVEGLPERF 153
           Q +DP+A HGVT FSDL+  EF R  +GV+GG    ++      A +      + LPE F
Sbjct: 89  QKMDPSARHGVTQFSDLTRSEFRRKHLGVKGGFKLPKD------ANQAPILPTQNLPEEF 148

Query: 154 DWREKGAVTGVKMQ----------------------------------------CDAADK 213
           DWR++GAVT VK Q                                        CD  ++
Sbjct: 149 DWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHECDPEEE 208

Query: 214 TACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGR-RGQCNFQSDKIAVRVSNFTTIPI 273
            +CD+GC+GGLM +A++Y + +GGL  E  YPYTG   G C     KI   VSNF+ + I
Sbjct: 209 GSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCKLDRSKIVASVSNFSVVSI 268

Query: 274 DEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLLVGYGDEGFSILR 333
           +EDQIAA+L++ GPLAV +NA +MQTYIGGVSCP IC +R +NHGVLLVGYG  GFS  R
Sbjct: 269 NEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRR-LNHGVLLVGYGSAGFSQAR 328

[Close]

BLAST of Spg010060 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 297.7 bits (761), Expect = 1.1e-80
Identity = 156/341 (45.75%), Postives = 204/341 (59.82%), Query Frame = 0


Query: 35  LRQVTDGDDDGEIINRLPLGSERKFVTFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQ 94
           +RQV   ++D +++N     +E  F  F  KY K+Y T+ E+ HR  +F  NL RA  +Q
Sbjct: 36  IRQVVPEENDEQLLN-----AEHHFTLFKSKYEKTYATQVEHDHRFRVFKANLRRARRNQ 95

Query: 95  ALDPTAVHGVTPFSDLSEEEFERMFMGVRGGAGGAEELLERNRAAEMTAEEVEGLPERFD 154
            LDP+AVHGVT FSDL+ +EF R F+G++    G     +   A  +   +   LP  FD
Sbjct: 96  LLDPSAVHGVTQFSDLTPKEFRRKFLGLK--RRGFRLPTDTQTAPILPTSD---LPTEFD 155

Query: 155 WREKGAVTGVKMQ----------------------------------------CDAADKT 214
           WRE+GAVT VK Q                                        CD A   
Sbjct: 156 WREQGAVTPVKNQGMCGSCWSFSAIGALEGAHFLATKELVSLSEQQLVDCDHECDPAQAN 215

Query: 215 ACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGR-RGQCNFQSDKIAVRVSNFTTIPID 274
           +CD+GC GGLM NA++Y + +GGL +E  YPYTGR    C F   KI   VSNF+ +  D
Sbjct: 216 SCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYTGRDHTACKFDKSKIVASVSNFSVVSSD 275

Query: 275 EDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLLVGYGDEGFSILRL 334
           EDQIAA+LV+ GPLA+ +NA++MQTYIGGVSCP +C K   +HGVLLVG+G  G++ +RL
Sbjct: 276 EDQIAANLVQHGPLAIAINAMWMQTYIGGVSCPYVCSKS-QDHGVLLVGFGSSGYAPIRL 335

[Close]

BLAST of Spg010060 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 292.7 bits (748), Expect = 3.7e-79
Identity = 155/345 (44.93%), Postives = 205/345 (59.42%), Query Frame = 0


Query: 38  VTDGDDDGEIINRLPLG--------SERKFVTFMEKYGKSYPTRKEYLHRLGIFAKNLIR 97
           V DGDD   ++ R  +G        SE  F  F  K+GK Y + +E+ +R  +F  NL R
Sbjct: 25  VNDGDD---LVIRQVVGGAEPQVLTSEDHFSLFKRKFGKVYASNEEHDYRFSVFKANLRR 84

Query: 98  AAEHQALDPTAVHGVTPFSDLSEEEFERMFMGVRGGAGGAEELLERNRAAEMTAEEVEGL 157
           A  HQ LDP+A HGVT FSDL+  EF +  +GVR G    +   + N+A  +     E L
Sbjct: 85  ARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGVRSGFKLPK---DANKAPIL---PTENL 144

Query: 158 PERFDWREKGAVTGVKMQ----------------------------------------CD 217
           PE FDWR+ GAVT VK Q                                        CD
Sbjct: 145 PEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHECD 204

Query: 218 AADKTACDNGCHGGLMTNAYKYLIDSGGLEEEASYPYTGRRGQ-CNFQSDKIAVRVSNFT 277
             +  +CD+GC+GGLM +A++Y + +GGL +E  YPYTG+ G+ C     KI   VSNF+
Sbjct: 205 PEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPYTGKDGKTCKLDKSKIVASVSNFS 264

Query: 278 TIPIDEDQIAAHLVRRGPLAVGLNAVFMQTYIGGVSCPLICGKRFVNHGVLLVGYGDEGF 334
            I IDE+QIAA+LV+ GPLAV +NA +MQTYIGGVSCP IC +R +NHGVLLVGYG  G+
Sbjct: 265 VISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSCPYICTRR-LNHGVLLVGYGAAGY 324

[Close]

BLAST of Spg010060 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 151.0 bits (380), Expect = 1.7e-36
Identity = 105/312 (33.65%), Postives = 152/312 (48.72%), Query Frame = 0


Query: 59  FVTFMEKYGKSYPTRKEYLHRLGIFAKNLIRAAEHQALDPTAVHGVTPFSDLSEEEFERM 118
           F  ++  + K+Y T +E   R  +F  NL    E      +   G+  F+DLS EEF++M
Sbjct: 51  FENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNEFADLSHEEFKKM 110

Query: 119 FMGVRGGAGGAEELLERNRAAEMTAEEVEGLPERFDWREKGAVTGVKMQ-----CDAADK 178
           ++G++      +E  ER+  AE    +VE +P+  DWR+KGAV  VK Q     C A   
Sbjct: 111 YLGLKTDIVRRDE--ERS-YAEFAYRDVEAVPKSVDWRKKGAVAEVKNQGSCGSCWAFST 170

Query: 179 TA-----------------------CD----NGCHGGLMTNAYKYLIDSGGLEEEASYPY 238
            A                       CD    NGC+GGLM  A++Y++ +GGL +E  YPY
Sbjct: 171 VAAVEGINKIVTGNLTTLSEQELIDCDTTYNNGCNGGLMDYAFEYIVKNGGLRKEEDYPY 230

Query: 239 TGRRGQCNFQSDKI-AVRVSNFTTIPIDEDQIAAHLVRRGPLAVGLNAV--FMQTYIGGV 298
           +   G C  Q D+   V ++    +P ++++     +   PL+V ++A     Q Y GGV
Sbjct: 231 SMEEGTCEMQKDESETVTINGHQDVPTNDEKSLLKALAHQPLSVAIDASGREFQFYSGGV 290

Query: 299 SCPLICGKRFVNHGVLLVGYGDEGFSILRLRKLPYWIIKNSWGEGWGERGYYRLCRG--- 332
                CG   ++HGV  VGYG    S        Y I+KNSWG  WGE+GY RL R    
Sbjct: 291 FDGR-CGVD-LDHGVAAVGYGSSKGS-------DYIIVKNSWGPKWGEKGYIRLKRNTGK 350

The following BLAST results are available for this feature:

BLAST of Spg010060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892721.1	7.8e-164	79.31	probable cysteine protease RD19D [Benincasa hispida]	[more]
XP_023535677.1	3.4e-159	78.25	probable cysteine protease RD19D [Cucurbita pepo subsp. pepo]	[more]
XP_022956816.1	4.4e-159	78.25	probable cysteine protease RD19D [Cucurbita moschata]	[more]
KAG7031982.1	5.8e-159	78.25	putative cysteine protease RD19D, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_004142497.3	1.4e-157	76.39	probable cysteine protease RD19D [Cucumis sativus] >KAE8648852.1 hypothetical pr...	[more]

BLAST of Spg010060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYS0	2.2e-113	58.86	Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 S...	[more]
Q10716	5.7e-85	45.58	Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1	[more]
P25804	3.1e-83	44.41	Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P43295	3.2e-80	44.57	Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S...	[more]
Q9SUL1	1.6e-79	45.75	Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S...	[more]

BLAST of Spg010060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GXJ8	2.1e-159	78.25	probable cysteine protease RD19D OS=Cucurbita moschata OX=3662 GN=LOC111458402 P...	[more]
A0A0A0KTI9	6.9e-158	76.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622590 PE=3 SV=1	[more]
A0A6J1JBC1	4.4e-157	76.92	probable cysteine protease RD19D OS=Cucurbita maxima OX=3661 GN=LOC111482910 PE=...	[more]
A0A5D3CBQ6	5.8e-157	76.66	Cysteine proteinase 15A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3BG12	5.8e-157	76.66	cysteine proteinase 15A OS=Cucumis melo OX=3656 GN=LOC103489441 PE=3 SV=1	[more]

BLAST of Spg010060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G54940.2	1.6e-114	58.86	Papain family cysteine protease	[more]
AT2G21430.1	2.3e-81	44.57	Papain family cysteine protease	[more]
AT4G16190.1	1.1e-80	45.75	Papain family cysteine protease	[more]
AT4G39090.1	3.7e-79	44.93	Papain family cysteine protease	[more]
AT1G20850.1	1.7e-36	33.65	xylem cysteine peptidase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 129..149
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 169..335 e-value: 3.8E-47 score: 163.4
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 54..168 e-value: 8.3E-22 score: 80.1
None	No IPR available	PANTHER	PTHR12411:SF783	CYSTEINE PROTEASE RD19C-RELATED	coord: 171..334 coord: 47..166
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 171..334 coord: 47..166
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 59..115 e-value: 5.8E-18 score: 75.7
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 59..115 e-value: 1.3E-11 score: 44.8
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 149..334 e-value: 1.4E-58 score: 210.6
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 175..333 e-value: 2.3E-42 score: 145.6
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 298..317
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 274..284
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 150..333 e-value: 3.99456E-67 score: 207.862
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 54..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg010060.1	Spg010060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity