Spg010036 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg010036
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Homocysteine S-methyltransferase family protein
Location	scaffold7: 4144746 .. 4151083 (+)
RNA-Seq Expression	Spg010036
Synteny	Spg010036

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATACGAACAAGCCGACGCCCGGAGAATCTGAGTGCAAACGCGTCCATGTTCATAGACAGAGCGACAATATCCATATTGACTTTGTCGGCCAATCCCGTCCCCCGTTGTCACTGGCTTTAATTGCGTATCGAATTTCAAAGGAGCGAAGAAGAAGAAGAGAAGCTGCGAGGCAGACAGAGAGAGGAAATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGCGGTCATTGATGGAGGTCTCGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGCGCTGTTTGCCTCATTAAAGATCCTGACCTAATCAAGAAGGTCCTCTCTTACCGTCTCTGTTTCCTTTGTTTCGGTGCATCACGTTCTCATCGGTGCTTAATGATCGGATTAATTTTTCAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGTTTGTAATGCGGATGGACTGAGTTTTATTTATACCTTTCTCAACTGCATACATGAAAATTTTTGTACAATGCAGTCTGTGACGGCTTTTGCAGTACCTTTTTCTTTTAAAAGTTGAACTTTGGTTTGTTTAAAACTTGATTCGAATTGGATATTTTAATGCTAATGAACAGAATGCATGAGGATCAAAGTGATAACTCCTCTCTAATTGAAGTTAAAGTTCAAAATGAGCCATTTCTATTGTGTCGTGCGATGATGAACCAAAACTGAACCGGTGTGTGTACACTCAACGAATGTGGAGAATTTAATCAAGTTAAAAGCATCGGATTAAAGTGCTTTCTATGGAGTAACATTTGTGGACATTCTAGGTGTTGTAATTGGTGGGCCAAATTATGCTTGCATTATAATTTGTAACGGACGTTGCTAAAAGCGACATTACATAGTCGACAGTATTTAATATCTGGAAGGGAAATGTTTGTGTGCTTACTAATATATGACCACATTGGTTTAGCTCAGTTGTCCAAAATGCAAGCTATAAATCAAGATGCCTCTTCTAAATATATCACCACCTATCTTTTTATAAGTCTGTCAGAATGCCATGCGACCATCCCAGCAGGACAAAGCCTGCCGAGAAGTTTTCCAATGCCAAATGTTGTAGAATTATGTGATCAGTTCATGAGATTTATTGAGATGCATGTAATTTGGCTTTATTACACAGTTAGTTTGATTTGTTATAGAGGCAGTTTCTAATCGTTAGCTTTCTGTTATTCTTCATTGTATTTAAAGACTGAGGGATTTCATTTAATCTTGTAAGAGTTCTTATAGTTAATAAAAACTCTCTTCCCTCACTCACACTTGTTAGGCTTGTACACCTAATGAATAAAAATAATGTGCTTACTCATATTTAGTTTGTGATGTTATATAATATAGCCATCTTGTAAAAGAGCTTCTTTTTGCTATTGAAAATTTCAATAGATATTTGCTTCTGTATAGCTTAGGATTTGTATGAACCAATATATGCCCTCCTCTTAATGAATTTTCTGTTTTCACAACTTAATATACCATATCAGAATTCTCGATACTTTATTAGAATTTAGACCCGCATAATATTGGAGTGCAAAATGTGACACCATTTTGTGCTTAAAATTTCTTAAGAATTCCAATAATCTTTAGTATGTATTTTGCCATGCTTAAATTTTTTTTATAGAAATTATTCCTTCTGTTAAAAATTATAAACAGAATACCAATAATGTACACCAATGTTCAAGCCAGCTAATTACTCTCTCTTGTCACAAATTTTATTCTATTACAGGCCACCATTCCTGGGTTTTTATCAAAGGGGTTCTCCGTGGAAGAAGCAGAGTTGCTCTTGGAAAAGAGTGTTAAATTGGCAGTGGAAGCTCGGGATAATTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGGTCGGAATACAGGTAACTGATACATATGAAACATTTAAATAAGGAGAGTTTGTAAATATATGACATTCGGAGCTAATCCTATTCCAACCCCCCCTCCCCTAACCAGTGGGCATTATGGGCCAGATGTGAATGTGGATAAGCTGAAGGACTTTCATCGACGCAGATTGCAGATTCTTGTCGATGCAGGCCCAGATTTGCTTGCATTTGAGACTATTCCGAATAAACTTGAAAGCCAGGTTTTTTTTTAATGCCAACTCTTCTTATATCATCTTAACTGTGCTTGAATAATAGACTTACTCTTACTGCCACACTGATGTTCCCACTGTAACACTTGTATTGTTATGCTTTGATCTGAGTCATAAAATATTTTAGGAAACAAGTTATGGTTTATTTTTTAATCTGTTCGTCTGTATTTATGAGTTGTACAAGGGATCAAGGTAATTTGACAACCATCATGGCATCGTCAATGGGGGGCATCCATTGCATGAGTAGGTCTTAATTTATGAACTGCTTAATTGGCTATCTTGTTCGTGATCAAATTTGTTTTAAATCCCATTCAAGTTCTAAAATTGATCCCAATTTAATAGTTATATTTTCAGATATTCTATTTTAATTTTCAAACTAGTATTATAACATTTGATTTTGTTGGTCTTGTCTGCCACAACATACTGTTAAGAAGTGGAAGGAAAATGTTGAATGTTTCTTATTACTGTCACGAGGATATGAAGACACTTGTTTCAATTGTTTTTCCAATTTGCTTACGTTTGTCTTTCCTCTCTGTGATTCTGTTTCCAACCAATCTTACGTTTGTTCCCCCTCCCTTCTGGTCTAATTAAAAACATTAGGCGTGCGTGGAGTTGCTCGAAGAAGAAAACATCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTCAGAATGCCCCATCTGGAGAGAGCTTCGAGGAGTGCCTCCGTGCTATTAATAAAAGCGACAAAGTAAATGCAGTTGGCATAAACTGTACTCCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGGTAGGAACCAAATCTCTTATTGCTGATTTGATTTTGTTTTCTTGTTCACTGGTTTTGACACCAAATTTTTTCCTTCAGTTAACAGACAAGCCGATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGGTGAGTCAACTTATCCTCCCCTCCCCCATTGTGATATTTCCATGGCTTGTGGGCCTACATTGTGATATTTCCATAACTAATTTAGTCTCTGGTTTTTGTAGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCTAAATTCATTGGTGGCTGTTGTCGAACGACACCATCTATGATCCGAGCAGTATCAAAGATTTTGAAAGAAAGCTCTCACTAGAGACAACTCATAAGTGGACTGTCTGTTGCTGCAGGGGACTATTGAATGAACGTAAAGATTATATACTAAAGCTAGAGGCAAGTTGGTCAGTACCTTCAAAGTACTTTCTTTGGAATAATTTTTAACCTTTATTTTTTTGTAAACTACTAGATTGAGGTGTTACGATTTGTACTACAACATACTTTGTCGGCTTTATAGATTTTTACTTTATCAAATTCGAAAGGCTTGTTAAAGAAATTAGGCATTTTCTCCTATTCATGATATTCAACTAAAATTGAGGAAAAGAAAACTATTACATTTTCAGAACATTTTTTATATTTTATTCCAAAGAATGATAAAAATCTTAGAGAGGGGATCGATCTGGACGATGCAAACTTTGGATGCTGAGGCAGAACTTGGAAGTTTATAAGCAGAAACATAAAAACCGACAGAGTTCCAATCCCTTATGGATTAGGAAATCCATATATATCAGAATTTTCCCCAATAGTCCCAATCTTAGTTGCATATAAAATACTTGCAAGTGTGCATATATATATATAAATAAAATCTCGACTGATTTGAGCAACTCGAGCTGGGCTGAGAAAATCTTTTCTTTTCTGGGAAAGGATAAAAGTTGAGGAACAATTGTTTATCCTCAACGTAGACTGAAAGGAAGATGCCGAAAAGTATGGGATTAGACAGAAGGCAATGGATAATGCTGTTTGGGATGGGGGACCCTAACAAAATCTCAACTTTTAGTTAGACCAAAAATAAAAAGTGTCCCTTTTCACAGGAAGAAAGGTGAGTGATGGGTTGGTTTCGGAATTTTCCTCATCCCTCTTAATTAGGGTACCCACAAATTTACGCAGCCAATCAGAGAACAAGCCTTTGGATGAACTTCAATCCCTCGACCAATGAAAAAGTGGATAAGGCCCTTCTGTGGGCCCAGACTCTGCAATTCCTTTTTTTAGTAAAAAAGTGCCCAAAAGTGCAATTCCTCCCCAGTCTAGACCCCAGTCGAGAGCCTTCTCTGTTGTGAATTTAACTGTAAAAGCGCAGTCTGTGGGCCATTCCCCTGGCCCCTCGATGCTGTCTTGTTCAAATGGTTTCTCGTGAACATTTATAAAAAAACAGACGAACTTGTAGAAAATATCAGTCTCCAGCTCTATGTAGTGTCCAATTTCAAGCCTGCAAGTTGAGTTTCTGATACAAACTCACATTCTCATCTTTCTGGCTATTTGAATTCAATGACGACGAGGAAAAGCGTGGAGAAAACAGCTGAGGGTGGCAAGACCGCCAGAGCCTGCGATAGCTGTATCAGCAAGCGGGCTCGGTGGTACTGTGCCGCTGATGATGCTTTTCTGTGCCAAGCTTGTGATGCTTCGGTCCACTCCGCCAACTCCTTGGCTCGTAGGCACGAGCGAGTTCGTCTTCAAACTGCCTCTTTCAGATCCTCTTCCGACAAGTCCTCTGCTGCCTCATGGCACCAGGGCTTCACCAGAAAGCCACGCTCGCCTCGAATGGGAAAGATTTCTCTGGCGAGGAAGCCATTTCCTCAGGTACCGGAGGTGAGTGCTAAAGAAGAAACTGAGGATCATGAGCAGCTCCTCCTTCATCGAGTTCCTGTATTGGGAGCCGATTCGAAAGACGGTAATTTGGCTAGTTTTGTTGGGGAGAGAGAAAACTCAAATGGATATCTTTCGTATGATATGGATCCTGCTGAATTTGCGGCTGATGTTGAGAGTTTGTTGGGGAACTCGCTTGATAATGAATGCTTTGATATGGAAGAATTGGGGCTGGCAGCTTCCAAAGACGACTCGACGACGAAGGATGATTACTCTTTTAACAGCTGCGAAGTTATTAAGATTGAACCAGATGAGATTGAGGATCTTAATCCGATGCTCGGTATAGTGGCCGATACGATGAGGGAGCCATTTGAGCTGAACTTTATGGACTTCGGCAGCAATCCGACGTCCTGCGGCGAGGAAGAGAAGGTGATGGTGGAAGTGATGGCAGTGGTGAAGAACGGTGATCTTGAGATGGAAGAGACGAAGATTGTAAAAAACAAAAAGAAAGTATCATTGAGTTTGGATAGTGAAGCAGTGATAATAGCTTGGGGGAGCAGAGGAACTCCGTGGACGTCCGGTGATCGGCCGAATCTCGACCTCGATTATTACTGGCCGGACTACATGGTTTGTTCCCTTCTCTTCTTCTCTATGTCCTATGATATTTATTATCTTATGCTGTGGTTTAAGAATGTTGTGTGTATAATAAATGCTCTGTCTCTGAAAGTGAAGTGTGTTATTATGAAGGGTACGTGCGAGAGTGAATATTATTATCCCGCTTACGGGGAGGTTGGCGGCGGAATCAGCCGACAAGCGGCGGCCGGAGTTGAAGGAGAGAGAGAAGCACGAGTGTCGAGATACAGAGAGAAGAGAAGGACGAGATTATTTGCAAAGAAAATAAGATATGAAGTTCGGAAATTGAATGCAGAGAAGAGACCAAGAATGAAAGGGAGGTTTGTGAAGAGATCGTCTTGCTTTGCGCCACCACCTTTGCCTTTGTTTAATTAATGATTATTAACATCATAAAGATATATATTTCCAATTTGTTCCCGTTCGTAATTTAATCCCCAATCTTGATTTTGTACCACTAATTTAAAAAATAATGCCTTTTATCCAAGTTAAGGTCACTATATTTATGGAAAAGTCACACTCGAATTCTCTATCTTTTGTTGGAAATTTCTAATAATGTCTTGGAGTTAAAATAGATCCTCAATCTTTTTTTTTTTTTTTTTTTTTACAATATGTGAGGGTGAGGGATTTGAAACACAGACCTCTTGGTCATTAGCACAAACTTTATGTCAGTTGAACTATACTAATGTTGATTCAATTGAAAGATGATAGATCCTT

mRNA sequence

ATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGCGGTCATTGATGGAGGTCTCGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGCGCTGTTTGCCTCATTAAAGATCCTGACCTAATCAAGAAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGCCACCATTCCTGGGTTTTTATCAAAGGGGTTCTCCGTGGAAGAAGCAGAGTTGCTCTTGGAAAAGAGTGTTAAATTGGCAGTGGAAGCTCGGGATAATTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGGTCGGAATACAGTGGGCATTATGGGCCAGATGTGAATGTGGATAAGCTGAAGGACTTTCATCGACGCAGATTGCAGATTCTTGTCGATGCAGGCCCAGATTTGCTTGCATTTGAGACTATTCCGAATAAACTTGAAAGCCAGGCGTGCGTGGAGTTGCTCGAAGAAGAAAACATCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTCAGAATGCCCCATCTGGAGAGAGCTTCGAGGAGTGCCTCCGTGCTATTAATAAAAGCGACAAAGTAAATGCAGTTGGCATAAACTGTACTCCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGGTAGGAACCAAATCTCTTATTGCTGATTTGATTTTGTTTTCTTGTTCACTGGTTTTGACACCAAATTTTTTCCTTCAGTTAACAGACAAGCCGATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCTAAATTCATTGGTGGCTGTTGTCGAACGACACCATCTATGATCCGAGCAGTATCAAAGATTTTGAAAGAAAGCTCTCACTAG

Coding sequence (CDS)

ATGGGGATGAAGAAGGCGACGACATTGTTGGATGATCTGTTACAGAACGCTGGAGGTTGTGCGGTCATTGATGGAGGTCTCGCCACGCAGCTTGAGAAACACGGCGCTGTCTTTAACGATTCACTTTGGAGCGCTGTTTGCCTCATTAAAGATCCTGACCTAATCAAGAAGGTCCATTTGGAGTACTTGGAGGCTGGAGCAGATATTTTGGTTTCTTCATCTTACCAGGCCACCATTCCTGGGTTTTTATCAAAGGGGTTCTCCGTGGAAGAAGCAGAGTTGCTCTTGGAAAAGAGTGTTAAATTGGCAGTGGAAGCTCGGGATAATTTCTGGGATTCTGTGAAGAGTATCCCAGGGCATAATTACAATAGGGCTTTGGTTGCAGCATCCATTGGAAGCTATGGTGCTTATCTTGCTGATGGGTCGGAATACAGTGGGCATTATGGGCCAGATGTGAATGTGGATAAGCTGAAGGACTTTCATCGACGCAGATTGCAGATTCTTGTCGATGCAGGCCCAGATTTGCTTGCATTTGAGACTATTCCGAATAAACTTGAAAGCCAGGCGTGCGTGGAGTTGCTCGAAGAAGAAAACATCCAAATTCCATCTTGGATCTGCTTTAGCTCCGTAGATGGTCAGAATGCCCCATCTGGAGAGAGCTTCGAGGAGTGCCTCCGTGCTATTAATAAAAGCGACAAAGTAAATGCAGTTGGCATAAACTGTACTCCTCCTCATTTTATTGAAGCTCTCATTTGTAAGTTCAAAGAGGTAGGAACCAAATCTCTTATTGCTGATTTGATTTTGTTTTCTTGTTCACTGGTTTTGACACCAAATTTTTTCCTTCAGTTAACAGACAAGCCGATAGTTGTCTATCCCAATAGTGGCGAGGTATGGGATGGCAGAGCCAAGAAATGGCTGCCATCAAATTGTTTTGGTGATGACAAATTTGAATCACTTAGTTCAAGATGGCGTGATTTGGGAGCTAAATTCATTGGTGGCTGTTGTCGAACGACACCATCTATGATCCGAGCAGTATCAAAGATTTTGAAAGAAAGCTCTCACTAG

Protein sequence

MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGHNYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFETIPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGINCTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH

Homology

[Close]

BLAST of Spg010036 vs. NCBI nr
Match: XP_023541078.1 (homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 632.9 bits (1631), Expect = 1.7e-177
Identity = 309/354 (87.29%), Postives = 319/354 (90.11%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKATTLLDDL+QNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LKDFHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALICKFKE                          LTDKPIVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALICKFKE--------------------------LTDKPIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH 355
           RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Spg010036 vs. NCBI nr
Match: KAG6573963.1 (Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013023.1 Homocysteine S-methyltransferase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 627.5 bits (1617), Expect = 7.1e-176
Identity = 307/354 (86.72%), Postives = 318/354 (89.83%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKATTLLDDL+QNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PG+
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGY 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LK FHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALICKFKE                          LTDKPIVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALICKFKE--------------------------LTDKPIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH 355
           RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Spg010036 vs. NCBI nr
Match: XP_022945567.1 (homocysteine S-methyltransferase 1 [Cucurbita moschata])

HSP 1 Score: 627.1 bits (1616), Expect = 9.3e-176
Identity = 306/354 (86.44%), Postives = 317/354 (89.55%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKATTLLDDL+QNAGGC VIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAG+DILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LK FHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALICKFKE                          LTDKPIVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALICKFKE--------------------------LTDKPIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH 355
           RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Spg010036 vs. NCBI nr
Match: XP_022968494.1 (homocysteine S-methyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 625.5 bits (1612), Expect = 2.7e-175
Identity = 306/354 (86.44%), Postives = 317/354 (89.55%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKA TLLDDL+QNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LKDFHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QI SWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALICKFKE                          LTDKPIVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALICKFKE--------------------------LTDKPIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH 355
           RAKKWLPSNCFGDDKFESLS+RWRDLGAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 RAKKWLPSNCFGDDKFESLSARWRDLGAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Spg010036 vs. NCBI nr
Match: XP_022150665.1 (homocysteine S-methyltransferase 1 [Momordica charantia])

HSP 1 Score: 616.7 bits (1589), Expect = 1.3e-172
Identity = 302/353 (85.55%), Postives = 315/353 (89.24%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M MKKATTLLDDL++NAGGCAVIDGG ATQLEKHGAV ND LWSAVCLIKDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+VSSSYQATIPGFLSKG S EE ELLLEKSVKLAVEARD+FW+SVKSIPGH
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSG+YGPDVNVDKLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECLRAINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALICKFKE                          LTDKPIVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALICKFKE--------------------------LTDKPIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESS 354
           RAKKWLPSNCFGDDKFES SSRWRDLGAKFIGGCCRTTPS  RAVSK+LKESS
Sbjct: 301 RAKKWLPSNCFGDDKFESFSSRWRDLGAKFIGGCCRTTPSTTRAVSKVLKESS 327

[Close]

BLAST of Spg010036 vs. ExPASy Swiss-Prot
Match: A4ZGQ8 (Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN=HMT1 PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-144
Identity = 242/350 (69.14%), Postives = 285/350 (81.43%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MG++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIKDP+LIK+VH+
Sbjct: 1   MGLEKKSALLEDLIEKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKDPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGAD++V+SSYQATIPGFLS+G S+EE+E LL+KSVKLAVEARD FWD V    GH
Sbjct: 61  EYLEAGADVVVTSSYQATIPGFLSRGLSMEESESLLQKSVKLAVEARDRFWDKVSKTSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSG YG DV++DKLKDFHRRR+Q+LV+A PDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGSYGEDVSLDKLKDFHRRRIQVLVEASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIP+WICF+SVDG+NAPSGESF+ECL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPAWICFTSVDGENAPSGESFQECLETLNKSNNICAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           C PP F++ LI KF                           +LT K IVVYPNSGEVWDG
Sbjct: 241 CAPPQFMDNLIRKFS--------------------------KLTQKAIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILK 351
           +AKKWLPS CFGD +FE  +++WRDLGAK IGGCCRTTPS I+A+S+ LK
Sbjct: 301 KAKKWLPSQCFGDAEFEMFATKWRDLGAKLIGGCCRTTPSTIKAISRDLK 324

[Close]

BLAST of Spg010036 vs. ExPASy Swiss-Prot
Match: Q9SDL7 (Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 9.7e-144
Identity = 242/350 (69.14%), Postives = 285/350 (81.43%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M ++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIK+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGFLS+G S+EE+E LL+KSV+LAVEARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+AGPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEE +QIP+WICF+SVDG+ APSGESFEECL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           C PP FIE LI K                          F +LT K IVVYPNSGEVWDG
Sbjct: 241 CAPPQFIENLIRK--------------------------FAKLTKKAIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILK 351
           +AK+WLPS CFGDD+FE  +++WRDLGAK IGGCCRTTPS I A+S+ LK
Sbjct: 301 KAKQWLPSQCFGDDEFEMFATKWRDLGAKLIGGCCRTTPSTINAISRDLK 324

[Close]

BLAST of Spg010036 vs. ExPASy Swiss-Prot
Match: Q9FUN0 (Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 9.1e-126
Identity = 218/342 (63.74%), Postives = 255/342 (74.56%), Query Frame = 0


Query: 9   LLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYLEAGAD 68
           +L+DL+  AGGCAVIDGG ATQLE  GA  ND LWSA CLI  P L+K+VH++YLEAGAD
Sbjct: 3   VLEDLVARAGGCAVIDGGFATQLEALGADINDPLWSAACLITRPHLVKEVHMQYLEAGAD 62

Query: 69  ILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGHNYNRALVA 128
           +++SSSYQATIPGF+++G SV EAE LL  SVKLA EARD FW S        YNRALVA
Sbjct: 63  VIISSSYQATIPGFIARGMSVAEAEDLLRTSVKLANEARDEFWKSTLRKSKPIYNRALVA 122

Query: 129 ASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFETIPNKLESQ 188
           ASIGSYGAYLADGSEYSG YG D+  +KLKDFHRRRLQ+L  AGPDL+AFE IPN++E+Q
Sbjct: 123 ASIGSYGAYLADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNQMEAQ 182

Query: 189 ACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGINCTPPHFIE 248
           A VELLEEE +QIPSWICFSSVDG+N  SGESF +CL+ +N S+KV  VG+NCTPP FIE
Sbjct: 183 ALVELLEEEKVQIPSWICFSSVDGKNLCSGESFADCLKILNASEKVAVVGVNCTPPQFIE 242

Query: 249 ALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDGRAKKWLPS 308
            +IC+F+                          + T K I VYPNSGEVWDGRAK+WLP 
Sbjct: 243 GIICEFR--------------------------KQTKKAIAVYPNSGEVWDGRAKRWLPV 302

Query: 309 NCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILK 351
            C G   F++L+ RW++ GA  IGGCCRTTPS IRAVSKILK
Sbjct: 303 ECLGHKSFDALAKRWQEAGASLIGGCCRTTPSTIRAVSKILK 318

[Close]

BLAST of Spg010036 vs. ExPASy Swiss-Prot
Match: Q9M1W4 (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.9e-99
Identity = 180/345 (52.17%), Postives = 232/345 (67.25%), Query Frame = 0


Query: 10  LDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYLEAGADI 69
           + D L+  GG AVIDGGLAT+ E+HGA  ND LWSA CL+  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 70  LVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGHN-----YNR 129
           + S+SYQATI GF +KGFS EE+E LL+KSV++A EAR++++D   +    +        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 130 ALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFETIPNK 189
            LVAAS+GSYGAYLADGSEYSG YG  + ++KLKDFHRRRLQ+L ++G DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 190 LESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGINCTPP 249
           +E+QA  +LLEE +++IP W  F+S DG N  SG+S +EC+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 250 HFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDGRAKK 309
            FIE L+ + ++V                          T KPI+VYPNSGE +D   K+
Sbjct: 249 RFIEGLVLEIEKV--------------------------TSKPILVYPNSGESYDADRKE 308

Query: 310 WLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKIL 350
           W+ +   GD+ F S   +W D G   +GGCCRTTP+ IRA+ K L
Sbjct: 309 WVENTGVGDEDFVSYVEKWMDAGVSLLGGCCRTTPTTIRAIHKRL 327

[Close]

BLAST of Spg010036 vs. ExPASy Swiss-Prot
Match: Q8LAX0 (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 SV=2)

HSP 1 Score: 362.5 bits (929), Expect = 5.6e-99
Identity = 183/357 (51.26%), Postives = 238/357 (66.67%), Query Frame = 0


Query: 4   KKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYL 63
           ++ ++L+ D L+  GG AV+DGG AT+L++HGA  ND LWSA CLI  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 64  EAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNF--------WDSVK 123
           E+GA+I++++SYQATI GF++KG SV EAE LL +SV++  EAR+ F        WD   
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDF-- 126

Query: 124 SIPGHNYNR-ALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPD 183
           +  G    R  LVAAS+GSYGAYLADGSEYSG YG  V+ + LKDFHRRR+QIL  +G D
Sbjct: 127 AYAGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGAD 186

Query: 184 LLAFETIPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKV 243
           L+AFETIPNKLE++A  +LLEEE+I IP+W  F+S DG + P G+S  EC +  +    V
Sbjct: 187 LIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNV 246

Query: 244 NAVGINCTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNS 303
            A+GINCT P +I ALI   +                          Q+T KPIVVYPNS
Sbjct: 247 VAIGINCTAPRYIHALIISLR--------------------------QMTRKPIVVYPNS 306

Query: 304 GEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKE 352
           GEV+DG  KKW+ S    ++ F S  S+WRD GA   GGCCRTTP+ IRA++K+L +
Sbjct: 307 GEVYDGLNKKWIKSEGESEEDFVSYVSKWRDAGASLFGGCCRTTPNTIRAIAKVLSD 335

[Close]

BLAST of Spg010036 vs. ExPASy TrEMBL
Match: A0A6J1G1A5 (homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.5e-176
Identity = 306/354 (86.44%), Postives = 317/354 (89.55%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKATTLLDDL+QNAGGC VIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKATTLLDDLIQNAGGCVVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAG+DILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGSDILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LK FHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKYFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEECLKAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALICKFKE                          LTDKPIVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALICKFKE--------------------------LTDKPIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH 355
           RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Spg010036 vs. ExPASy TrEMBL
Match: A0A6J1HZT4 (homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467714 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.3e-175
Identity = 306/354 (86.44%), Postives = 317/354 (89.55%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MGMKKA TLLDDL+QNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCL+KDPDLIKKVHL
Sbjct: 1   MGMKKAMTLLDDLIQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLVKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGFLSKG S EE ELLLE+SVKLAVEARD+FWDSVKS PGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFLSKGLSAEEGELLLERSVKLAVEARDSFWDSVKSNPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDV+VD LKDFHRRRLQILV A PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVDVDTLKDFHRRRLQILVGASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QI SWICFSSVDG+NAPSGESFEECL+AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQILSWICFSSVDGENAPSGESFEECLQAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALICKFKE                          LTDKPIVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALICKFKE--------------------------LTDKPIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH 355
           RAKKWLPSNCFGDDKFESLS+RWRDLGAKFIGGCCRTTPS IRAVSK+LKES H
Sbjct: 301 RAKKWLPSNCFGDDKFESLSARWRDLGAKFIGGCCRTTPSTIRAVSKVLKESPH 328

[Close]

BLAST of Spg010036 vs. ExPASy TrEMBL
Match: A0A6J1D951 (homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111018744 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.1e-173
Identity = 302/353 (85.55%), Postives = 315/353 (89.24%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M MKKATTLLDDL++NAGGCAVIDGG ATQLEKHGAV ND LWSAVCLIKDPDLIKKVHL
Sbjct: 1   MVMKKATTLLDDLIENAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLIKDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+VSSSYQATIPGFLSKG S EE ELLLEKSVKLAVEARD+FW+SVKSIPGH
Sbjct: 61  EYLEAGADIVVSSSYQATIPGFLSKGLSTEEGELLLEKSVKLAVEARDSFWNSVKSIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSG+YGPDVNVDKLKDFHRRRLQ+LV+A PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGNYGPDVNVDKLKDFHRRRLQVLVNACPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFEECLRAINKS+KVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGKNAPSGESFEECLRAINKSNKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALICKFKE                          LTDKPIVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALICKFKE--------------------------LTDKPIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESS 354
           RAKKWLPSNCFGDDKFES SSRWRDLGAKFIGGCCRTTPS  RAVSK+LKESS
Sbjct: 301 RAKKWLPSNCFGDDKFESFSSRWRDLGAKFIGGCCRTTPSTTRAVSKVLKESS 327

[Close]

BLAST of Spg010036 vs. ExPASy TrEMBL
Match: A0A1S3BEY1 (homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.7e-170
Identity = 298/354 (84.18%), Postives = 312/354 (88.14%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MG++KATTLLDDLL NAGGCAVIDGGLATQLEKHGAVFND LWSAVCLI DPDLIKKVHL
Sbjct: 1   MGIEKATTLLDDLLHNAGGCAVIDGGLATQLEKHGAVFNDPLWSAVCLINDPDLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILV+SSYQATIPGF+SKG SVEE ELLLEKSVKLA+EARD+FWDSVK IPGH
Sbjct: 61  EYLEAGADILVTSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRL+ILVDA PDLLAFET
Sbjct: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLRILVDASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEEN+QIPSWICFSSVDG+NAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENVQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALI KFKE                          LT+K IVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALITKFKE--------------------------LTNKHIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESSH 355
           R KKWLPSNCFGDDKFES +SRW  LGA FIGGCCRTTPS IRAVSK+LKESSH
Sbjct: 301 RFKKWLPSNCFGDDKFESFTSRWCKLGATFIGGCCRTTPSTIRAVSKVLKESSH 328

[Close]

BLAST of Spg010036 vs. ExPASy TrEMBL
Match: A0A0A0KR42 (Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660 PE=4 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.3e-169
Identity = 297/353 (84.14%), Postives = 308/353 (87.25%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           MG+KKATTLLDD L NAGGCAVIDGG ATQLEKHGAV ND LWSAVCLI DP LIKKVHL
Sbjct: 1   MGIKKATTLLDDFLHNAGGCAVIDGGFATQLEKHGAVINDPLWSAVCLINDPHLIKKVHL 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADILVSSSYQATIPGF+SKG SVEE ELLLEKSVKLA+EARD+FWDSVK IPGH
Sbjct: 61  EYLEAGADILVSSSYQATIPGFISKGLSVEEGELLLEKSVKLAIEARDSFWDSVKCIPGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
            YNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQI VDA PDLLAFET
Sbjct: 121 KYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQIFVDASPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEENIQIPSWICFSSVDG+NAPSGESFE+CL AINKSDKVNAVGIN
Sbjct: 181 IPNKLEAQACVELLEEENIQIPSWICFSSVDGENAPSGESFEKCLYAINKSDKVNAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           CTPPHFIEALI KFKE                          LT+K IVVYPNSGEVWDG
Sbjct: 241 CTPPHFIEALITKFKE--------------------------LTNKHIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKESS 354
           R KKWLPSNCFGDDKFESLSSRWR+LGA FIGGCCRTTPS IRAVSK+LKES+
Sbjct: 301 RFKKWLPSNCFGDDKFESLSSRWRNLGATFIGGCCRTTPSTIRAVSKVLKEST 327

[Close]

BLAST of Spg010036 vs. TAIR 10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 511.1 bits (1315), Expect = 6.9e-145
Identity = 242/350 (69.14%), Postives = 285/350 (81.43%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M ++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIK+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGFLS+G S+EE+E LL+KSV+LAVEARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+AGPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEE +QIP+WICF+SVDG+ APSGESFEECL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
           C PP FIE LI K                          F +LT K IVVYPNSGEVWDG
Sbjct: 241 CAPPQFIENLIRK--------------------------FAKLTKKAIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILK 351
           +AK+WLPS CFGDD+FE  +++WRDLGAK IGGCCRTTPS I A+S+ LK
Sbjct: 301 KAKQWLPSQCFGDDEFEMFATKWRDLGAKLIGGCCRTTPSTINAISRDLK 324

[Close]

BLAST of Spg010036 vs. TAIR 10
Match: AT3G25900.3 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 475.3 bits (1222), Expect = 4.2e-134
Identity = 228/350 (65.14%), Postives = 270/350 (77.14%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M ++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIK+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGFLS+G S+EE+E LL+KSV+LAVEARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+AGPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEE +QIP+WICF+SVDG+ APSGESFEECL  +NKS+ + A    
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA---- 240

Query: 241 CTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDG 300
                                                     LT K IVVYPNSGEVWDG
Sbjct: 241 ------------------------------------------LTKKAIVVYPNSGEVWDG 300

Query: 301 RAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILK 351
           +AK+WLPS CFGDD+FE  +++WRDLGAK IGGCCRTTPS I A+S+ LK
Sbjct: 301 KAKQWLPSQCFGDDEFEMFATKWRDLGAKLIGGCCRTTPSTINAISRDLK 304

[Close]

BLAST of Spg010036 vs. TAIR 10
Match: AT3G25900.2 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 408.7 bits (1049), Expect = 4.8e-114
Identity = 191/254 (75.20%), Postives = 225/254 (88.58%), Query Frame = 0


Query: 1   MGMKKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHL 60
           M ++K + LL+DL++  GGCAV+DGG ATQLE HGA  ND LWSAV LIK+P+LIK+VH+
Sbjct: 1   MVLEKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHM 60

Query: 61  EYLEAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGH 120
           EYLEAGADI+V+SSYQATIPGFLS+G S+EE+E LL+KSV+LAVEARD FW+ V  + GH
Sbjct: 61  EYLEAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVSGH 120

Query: 121 NYNRALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFET 180
           +YNRALVAASIGSYGAYLADGSEYSGHYG +V++DKLKDFHRRRLQ+LV+AGPDLLAFET
Sbjct: 121 SYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFET 180

Query: 181 IPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGIN 240
           IPNKLE+QACVELLEEE +QIP+WICF+SVDG+ APSGESFEECL  +NKS+ + AVGIN
Sbjct: 181 IPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGIN 240

Query: 241 CTPPHFIEALICKF 255
           C PP FIE LI KF
Sbjct: 241 CAPPQFIENLIRKF 254

[Close]

BLAST of Spg010036 vs. TAIR 10
Match: AT3G63250.1 (homocysteine methyltransferase 2 )

HSP 1 Score: 364.0 bits (933), Expect = 1.4e-100
Identity = 180/345 (52.17%), Postives = 232/345 (67.25%), Query Frame = 0


Query: 10  LDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYLEAGADI 69
           + D L+  GG AVIDGGLAT+ E+HGA  ND LWSA CL+  P LI  VHL+YLEAGADI
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 70  LVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNFWDSVKSIPGHN-----YNR 129
           + S+SYQATI GF +KGFS EE+E LL+KSV++A EAR++++D   +    +        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKILKKRP 128

Query: 130 ALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPDLLAFETIPNK 189
            LVAAS+GSYGAYLADGSEYSG YG  + ++KLKDFHRRRLQ+L ++G DL+AFETIPNK
Sbjct: 129 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 188

Query: 190 LESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKVNAVGINCTPP 249
           +E+QA  +LLEE +++IP W  F+S DG N  SG+S +EC+      +KV AVGINCTPP
Sbjct: 189 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 248

Query: 250 HFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNSGEVWDGRAKK 309
            FIE L+ + ++V                          T KPI+VYPNSGE +D   K+
Sbjct: 249 RFIEGLVLEIEKV--------------------------TSKPILVYPNSGESYDADRKE 308

Query: 310 WLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKIL 350
           W+ +   GD+ F S   +W D G   +GGCCRTTP+ IRA+ K L
Sbjct: 309 WVENTGVGDEDFVSYVEKWMDAGVSLLGGCCRTTPTTIRAIHKRL 327

[Close]

BLAST of Spg010036 vs. TAIR 10
Match: AT3G22740.1 (homocysteine S-methyltransferase 3 )

HSP 1 Score: 362.5 bits (929), Expect = 4.0e-100
Identity = 183/357 (51.26%), Postives = 238/357 (66.67%), Query Frame = 0


Query: 4   KKATTLLDDLLQNAGGCAVIDGGLATQLEKHGAVFNDSLWSAVCLIKDPDLIKKVHLEYL 63
           ++ ++L+ D L+  GG AV+DGG AT+L++HGA  ND LWSA CLI  P L+ KVHL+YL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 64  EAGADILVSSSYQATIPGFLSKGFSVEEAELLLEKSVKLAVEARDNF--------WDSVK 123
           E+GA+I++++SYQATI GF++KG SV EAE LL +SV++  EAR+ F        WD   
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDF-- 126

Query: 124 SIPGHNYNR-ALVAASIGSYGAYLADGSEYSGHYGPDVNVDKLKDFHRRRLQILVDAGPD 183
           +  G    R  LVAAS+GSYGAYLADGSEYSG YG  V+ + LKDFHRRR+QIL  +G D
Sbjct: 127 AYAGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGAD 186

Query: 184 LLAFETIPNKLESQACVELLEEENIQIPSWICFSSVDGQNAPSGESFEECLRAINKSDKV 243
           L+AFETIPNKLE++A  +LLEEE+I IP+W  F+S DG + P G+S  EC +  +    V
Sbjct: 187 LIAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNV 246

Query: 244 NAVGINCTPPHFIEALICKFKEVGTKSLIADLILFSCSLVLTPNFFLQLTDKPIVVYPNS 303
            A+GINCT P +I ALI   +                          Q+T KPIVVYPNS
Sbjct: 247 VAIGINCTAPRYIHALIISLR--------------------------QMTRKPIVVYPNS 306

Query: 304 GEVWDGRAKKWLPSNCFGDDKFESLSSRWRDLGAKFIGGCCRTTPSMIRAVSKILKE 352
           GEV+DG  KKW+ S    ++ F S  S+WRD GA   GGCCRTTP+ IRA++K+L +
Sbjct: 307 GEVYDGLNKKWIKSEGESEEDFVSYVSKWRDAGASLFGGCCRTTPNTIRAIAKVLSD 335

The following BLAST results are available for this feature:

BLAST of Spg010036 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023541078.1	1.7e-177	87.29	homocysteine S-methyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6573963.1	7.1e-176	86.72	Homocysteine S-methyltransferase 1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022945567.1	9.3e-176	86.44	homocysteine S-methyltransferase 1 [Cucurbita moschata]	[more]
XP_022968494.1	2.7e-175	86.44	homocysteine S-methyltransferase 1-like [Cucurbita maxima]	[more]
XP_022150665.1	1.3e-172	85.55	homocysteine S-methyltransferase 1 [Momordica charantia]	[more]

BLAST of Spg010036 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4ZGQ8	1.1e-144	69.14	Homocysteine S-methyltransferase 1 OS=Brassica oleracea var. italica OX=36774 GN...	[more]
Q9SDL7	9.7e-144	69.14	Homocysteine S-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=HMT-1 PE=1...	[more]
Q9FUN0	9.1e-126	63.74	Homocysteine S-methyltransferase 1 OS=Zea mays OX=4577 GN=HMT-1 PE=2 SV=1	[more]
Q9M1W4	1.9e-99	52.17	Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1...	[more]
Q8LAX0	5.6e-99	51.26	Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 ...	[more]

BLAST of Spg010036 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1A5	4.5e-176	86.44	homocysteine S-methyltransferase 1 OS=Cucurbita moschata OX=3662 GN=LOC111449762...	[more]
A0A6J1HZT4	1.3e-175	86.44	homocysteine S-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467...	[more]
A0A6J1D951	6.1e-173	85.55	homocysteine S-methyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC11101874...	[more]
A0A1S3BEY1	1.7e-170	84.18	homocysteine S-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103489284 PE=4 ...	[more]
A0A0A0KR42	5.3e-169	84.14	Hcy-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G609660...	[more]

BLAST of Spg010036 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25900.1	6.9e-145	69.14	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.3	4.2e-134	65.14	Homocysteine S-methyltransferase family protein	[more]
AT3G25900.2	4.8e-114	75.20	Homocysteine S-methyltransferase family protein	[more]
AT3G63250.1	1.4e-100	52.17	homocysteine methyltransferase 2	[more]
AT3G22740.1	4.0e-100	51.26	homocysteine S-methyltransferase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003726	Homocysteine-binding domain	PFAM	PF02574	S-methyl_trans	coord: 22..348 e-value: 2.7E-72 score: 243.9
IPR003726	Homocysteine-binding domain	PROSITE	PS50970	HCY	coord: 9..349 score: 46.858894
IPR036589	Homocysteine-binding domain superfamily	GENE3D	3.20.20.330		coord: 9..347 e-value: 3.0E-96 score: 324.4
IPR036589	Homocysteine-binding domain superfamily	SUPERFAMILY	82282	Homocysteine S-methyltransferase	coord: 17..351
IPR017226	Betaine-homocysteine S-methyltransferase, BHMT	PIRSF	PIRSF037505	BHMT	coord: 1..353 e-value: 3.4E-11 score: 40.9
None	No IPR available	PIRSR	PIRSR037505-2	PIRSR037505-2	coord: 9..242 e-value: 8.7E-10 score: 36.0
None	No IPR available	PANTHER	PTHR46015	ZGC:172121	coord: 1..352
None	No IPR available	PANTHER	PTHR46015:SF7	HOMOCYSTEINE S-METHYLTRANSFERASE 1	coord: 1..352

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg010036.1	Spg010036.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009086	methionine biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:0033528	S-methylmethionine cycle
molecular_function	GO:0047150	betaine-homocysteine S-methyltransferase activity
molecular_function	GO:0008898	S-adenosylmethionine-homocysteine S-methyltransferase activity
molecular_function	GO:0008270	zinc ion binding