Spg010031 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg010031 vs. NCBI nr
Match: XP_023531420.1 (WAT1-related protein At2g39510-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548.9 bits (1413), Expect = 3.2e-152
Identity = 291/377 (77.19%), Postives = 320/377 (84.88%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEG+ R   LAKPYL VVF+QFG+AGM+ILAKSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 1   MEGYGRLFELAKPYLVVVFVQFGYAGMTILAKSALDKGMSPHVFVVYRHAVATLVIAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           I+FDR                     PVIDQNLY+TGMK TTATF  AMCN++PAF+FLM
Sbjct: 61  IIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTATFTAAMCNILPAFSFLM 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 121 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 180

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 181 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 240

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW LHFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 241 NPAAWVLHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 300

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+FLGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 301 ILAEIMFLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 360

BLAST of Spg010031 vs. NCBI nr
Match: XP_022973899.1 (WAT1-related protein At2g39510-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 548.1 bits (1411), Expect = 5.5e-152
Identity = 292/377 (77.45%), Postives = 318/377 (84.35%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEGF R   LAKPYL VVF+QFG+AGM+ILAKSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 1   MEGFGRLFELAKPYLVVVFVQFGYAGMTILAKSALDKGMSPHVFVVYRHAVATLVIAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           I+FDR                     PVIDQNLY+TGMK TTATF  AMCN++PAF+FLM
Sbjct: 61  IIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTATFTAAMCNILPAFSFLM 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 121 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 180

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 181 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 240

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW LHFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 241 NPAAWVLHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 300

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+ LGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 301 ILAEIMLLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 360

BLAST of Spg010031 vs. NCBI nr
Match: XP_022943155.1 (WAT1-related protein At2g39510-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 546.6 bits (1407), Expect = 1.6e-151
Identity = 290/377 (76.92%), Postives = 318/377 (84.35%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEGF R   LAKPYL VVF+QFG+AGM+ILAKSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 1   MEGFGRLFELAKPYLVVVFVQFGYAGMTILAKSALDKGMSPHVFVVYRHAVATLVIAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           I+FDR                     PVIDQNLY+TGMK TTA F  AMCN++PAF+FLM
Sbjct: 61  IIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTAAFTAAMCNILPAFSFLM 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 121 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 180

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 181 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 240

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW  HFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 241 NPAAWVFHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 300

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+FLGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 301 ILAEIMFLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 360

BLAST of Spg010031 vs. NCBI nr
Match: KAG6600061.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 546.6 bits (1407), Expect = 1.6e-151
Identity = 289/377 (76.66%), Postives = 319/377 (84.62%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEGF R   LAKPYL VVF+QFG+AGM+ILAKSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 1   MEGFGRLFELAKPYLVVVFVQFGYAGMTILAKSALDKGMSPHVFVVYRHAVATLVIAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           ++FDR                     PVIDQNLY+TGMK TTATF  AMCN++PAF+FLM
Sbjct: 61  VIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTATFTAAMCNILPAFSFLM 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 121 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 180

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP++GSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 181 HQSPLEGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 240

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW  HFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 241 NPAAWVFHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 300

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+FLGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 301 ILAEIMFLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 360

BLAST of Spg010031 vs. NCBI nr
Match: XP_022973856.1 (WAT1-related protein At2g39510-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 545.8 bits (1405), Expect = 2.7e-151
Identity = 290/377 (76.92%), Postives = 317/377 (84.08%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           ME F R  GLAKPYL V+F+QFG+AGM+IL KSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 17  MERFGRLFGLAKPYLMVIFVQFGYAGMTILVKSALDKGMSPHVFVVYRYAVATLVIAPFA 76

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           I+FDR                     PVIDQNLY+TGMK TTATF  AMCN++PAF+FLM
Sbjct: 77  IIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTATFTAAMCNILPAFSFLM 136

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 137 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 196

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 197 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 256

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW LHFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 257 NPAAWVLHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 316

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+ LGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 317 ILAEIMLLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 376

BLAST of Spg010031 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 1.8e-105
Identity = 203/359 (56.55%), Postives = 258/359 (71.87%), Query Frame = 0

Query: 12  KPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFAIVFDR------ 71
           KP++ VV +QFG+AG+SI+AK AL++GMS +V   YR  VAT+ I PFA   DR      
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 72  ---------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLMAWVCRLEKVSI 131
                          P IDQNLYYTGMK+T+ATF  AM NV+PAFAF+MAW+ RLEKV++
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 132 LKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAHHQSPVKGSLMI 191
            K  SQAKILGTIVTVGGAM+MT ++GP++ LPW  P+     S      Q   KG+ +I
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGASLI 186

Query: 192 ATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERGNPSAWALHFDS 251
           A GCICWA FI LQAITLK+YP ELSLTA IC +G+I  + VAL +ERGNPSAWA+H DS
Sbjct: 187 AIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDS 246

Query: 252 QLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSFILSEILFLGRI 311
           +LLA VY GVICSG+ YY+QGV+M+T+GPVFVTAFNPLSM++VAI+ S IL+E++FLGRI
Sbjct: 247 KLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 306

Query: 312 IGAVSIIIGLYLVLWGKSKDQVSVK-SDCDKVTPSEQQMVSGKDEVSKTVESNEEFVVI 349
           +GA+ I++GLY VLWGKSKD+ S   SD DK  P     +    + +  +++N+  VVI
Sbjct: 307 LGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKELPLSTPQIVLPSKANAKMDTNDASVVI 365

BLAST of Spg010031 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 5.1e-92
Identity = 182/353 (51.56%), Postives = 247/353 (69.97%), Query Frame = 0

Query: 11  AKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFAIVFDR----- 70
           A+P++ +V +Q G AGM IL+K+ L+KGMS YV VVYR AVAT+V+ PFA  FD+     
Sbjct: 13  ARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPK 72

Query: 71  ----------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLMAWVCRLEKVS 130
                           PVIDQNLYY GMK+TTATFATAM NV+PA  F++A++  LE+V 
Sbjct: 73  MTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVK 132

Query: 131 ILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAHHQSPVKGSLM 190
           +    S  K++GT+ TVGGAMIMT ++GP+L+L WTK       +G   H  S +KG+++
Sbjct: 133 LRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTDIH--SAIKGAVL 192

Query: 191 IATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERGNPSAWALHFD 250
           +  GC  +A F+ LQAITL+ YPAELSLTA ICL+GTI G+ VAL+ME+GNPSAWA+ +D
Sbjct: 193 VTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWD 252

Query: 251 SQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSFILSEILFLGR 310
           ++LL   Y+G++CS + YY+ GVVM+T+GPVFVTAF+PL MI+VAIMS+ I +E ++LGR
Sbjct: 253 TKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLGR 312

Query: 311 IIGAVSIIIGLYLVLWGKSKD---QVSVKSDCDKVTPSEQQMVSGKDEVSKTV 340
           ++GAV I  GLYLV+WGK KD     +++ D +   P  +   +GKD V   V
Sbjct: 313 VLGAVVICAGLYLVIWGKGKDYKYNSTLQLDDESAQPKLELSGNGKDNVDHEV 363

BLAST of Spg010031 vs. ExPASy Swiss-Prot
Match: F4IQX1 (WAT1-related protein At2g37450 OS=Arabidopsis thaliana OX=3702 GN=At2g37450 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.8e-87
Identity = 171/322 (53.11%), Postives = 226/322 (70.19%), Query Frame = 0

Query: 11  AKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFAIVFDRPVIDQ 70
           A P++ +V +Q G+AGM IL K  L+KGMS YV  VYR  VAT+V+ PFA  FD PVI Q
Sbjct: 7   ALPFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFDNPVIAQ 66

Query: 71  NLYYTGMKFTTATFATAMCNVVPAFAFLMAWVCRLEKVSILKRGSQAKILGTIVTVGGAM 130
           NL+  GMK+TTATFA A+ N +PA  F++A + RLE V      S AK++GT+ TVGG M
Sbjct: 67  NLFNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVKFQSIRSAAKVVGTVTTVGGIM 126

Query: 131 IMTFIRGPMLNLPWTKPNQPSAPSGGAAHHQSPVKGSLMIATGCICWAAFITLQAITLKA 190
           +MT ++GP L+L WTK   PSA +       S +KG++++  GC  +A F+ LQAITLK 
Sbjct: 127 VMTLVKGPALDLFWTK--GPSAQNTVGTDIHSSIKGAVLVTIGCFSYACFMILQAITLKT 186

Query: 191 YPAELSLTALICLVGTIGGSGVALIMERGNPSAWALHFDSQLLAVVYAGVICSGVTYYIQ 250
           YPAELSL   ICL+GTI G  VAL+ME+GNPS WA+ +D++LL + Y+G++CS + YYI 
Sbjct: 187 YPAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWDTKLLTITYSGIVCSALGYYIG 246

Query: 251 GVVMQTKGPVFVTAFNPLSMILVAIMSSFILSEILFLGRIIGAVSIIIGLYLVLWGKSKD 310
           GVVM+T+GPVFVTAF PL MI+VAIMSS I  E ++LGR +GA  I +GLYLV+WGK+KD
Sbjct: 247 GVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGRALGATVICVGLYLVIWGKAKD 306

Query: 311 -----QVSVKSDCDKVTPSEQQ 328
                   +  D  + T S+Q+
Sbjct: 307 YEYPSTPQIDDDLAQATTSKQK 326

BLAST of Spg010031 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.8e-81
Identity = 161/365 (44.11%), Postives = 245/365 (67.12%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEG   ++   +PYL ++F+QFG AG  I+  + L++G ++YV +VYR  VA LV+ PFA
Sbjct: 1   MEGVSATMHKLRPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           ++F+R                     PV+DQ   Y GM  T+AT+ +A+ N++P+  F++
Sbjct: 61  LIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFII 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPN--QPSAPSGGA 180
           AW+ R+EKV+I +  S+AKI+GT+V +GGA++MT  +GP++ LPW+ PN  Q +  +  +
Sbjct: 121 AWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNS 180

Query: 181 AHHQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIME 240
             H + V G+L+I  GC+ W+ F  LQ+IT+K YPA+LSL+ALICL G +    VAL++E
Sbjct: 181 QDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVE 240

Query: 241 RGNPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMS 300
           R +PS WA+ +D++L A +Y G++ SG+TYY+QG+VM+T+GPVFVTAFNPL MILVA+++
Sbjct: 241 R-HPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIA 300

Query: 301 SFILSEILFLGRIIGAVSIIIGLYLVLWGKSKD-QVSVKSDCDKVTPSEQQMVSGKDEVS 342
           SFIL E +  G +IG   I  GLY+V+WGK KD +VS     +K +  E  + +  ++ +
Sbjct: 301 SFILHEQIHFGCVIGGAVIAAGLYMVVWGKGKDYEVSGLDILEKNSLQELPITTKSEDDN 360

BLAST of Spg010031 vs. ExPASy Swiss-Prot
Match: Q9FNA5 (WAT1-related protein At5g13670 OS=Arabidopsis thaliana OX=3702 GN=At5g13670 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.5e-78
Identity = 168/360 (46.67%), Postives = 235/360 (65.28%), Query Frame = 0

Query: 11  AKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFAIVFDR----- 70
           A+P++ +VF+Q  +A MSI+AK AL+KGMS +V V YR AVA+ +ITPFA++ +R     
Sbjct: 6   ARPFIAIVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRPK 65

Query: 71  ----------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLMAWVCRLEKVS 130
                           PV++QNLYY+GMK TTATF +A+CN +PA  F+MA V +LEKV+
Sbjct: 66  LTFKILLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVT 125

Query: 131 ILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH------HQSP 190
           I +R SQAK++GT+V +GGAM+MTF++G ++ LPWT     S    G  H          
Sbjct: 126 IERRHSQAKLVGTMVAIGGAMLMTFVKGNVIELPWT---SNSRGLNGHTHAMRIPKQADI 185

Query: 191 VKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERGNPSA 250
            +GS+M+   C  W+ +I LQA  L  Y AELSLTAL+C++G +  + + LI ER N S 
Sbjct: 186 ARGSIMLVASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMSV 245

Query: 251 WALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSFILSE 310
           W ++ D  LLA +Y G++ SG+ YY+ G   + +GPVFV+AFNPLSM+LVAI+S+F+  E
Sbjct: 246 WKINPDVTLLASIYGGLV-SGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVFLE 305

Query: 311 ILFLGRIIGAVSIIIGLYLVLWGKSKD------------QVSVKSDCDKV-TPSEQQMVS 331
            +++GR+IG+V I+IG+YLVLWGKSKD            +  VK D  KV TP   Q+VS
Sbjct: 306 KVYVGRVIGSVVIVIGIYLVLWGKSKDKGGMLQPNAGCAETVVKIDQQKVPTPDNNQVVS 361

BLAST of Spg010031 vs. ExPASy TrEMBL
Match: A0A6J1IFZ6 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111472335 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.7e-152
Identity = 292/377 (77.45%), Postives = 318/377 (84.35%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEGF R   LAKPYL VVF+QFG+AGM+ILAKSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 1   MEGFGRLFELAKPYLVVVFVQFGYAGMTILAKSALDKGMSPHVFVVYRHAVATLVIAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           I+FDR                     PVIDQNLY+TGMK TTATF  AMCN++PAF+FLM
Sbjct: 61  IIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTATFTAAMCNILPAFSFLM 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 121 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 180

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 181 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 240

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW LHFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 241 NPAAWVLHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 300

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+ LGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 301 ILAEIMLLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 360

BLAST of Spg010031 vs. ExPASy TrEMBL
Match: A0A6J1FXG1 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111447970 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 7.8e-152
Identity = 290/377 (76.92%), Postives = 318/377 (84.35%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEGF R   LAKPYL VVF+QFG+AGM+ILAKSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 1   MEGFGRLFELAKPYLVVVFVQFGYAGMTILAKSALDKGMSPHVFVVYRHAVATLVIAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           I+FDR                     PVIDQNLY+TGMK TTA F  AMCN++PAF+FLM
Sbjct: 61  IIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTAAFTAAMCNILPAFSFLM 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 121 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 180

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 181 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 240

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW  HFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 241 NPAAWVFHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 300

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+FLGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 301 ILAEIMFLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 360

BLAST of Spg010031 vs. ExPASy TrEMBL
Match: A0A6J1I9S6 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111472335 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.3e-151
Identity = 290/377 (76.92%), Postives = 317/377 (84.08%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           ME F R  GLAKPYL V+F+QFG+AGM+IL KSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 17  MERFGRLFGLAKPYLMVIFVQFGYAGMTILVKSALDKGMSPHVFVVYRYAVATLVIAPFA 76

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           I+FDR                     PVIDQNLY+TGMK TTATF  AMCN++PAF+FLM
Sbjct: 77  IIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTATFTAAMCNILPAFSFLM 136

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 137 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 196

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 197 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 256

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW LHFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 257 NPAAWVLHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 316

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+ LGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 317 ILAEIMLLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 376

BLAST of Spg010031 vs. ExPASy TrEMBL
Match: A0A6J1FQY4 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111447970 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.9e-151
Identity = 288/377 (76.39%), Postives = 317/377 (84.08%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           ME F R  GLAKPYL V+F+QFG+AGM+IL KSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 1   MERFGRLFGLAKPYLMVIFVQFGYAGMTILVKSALDKGMSPHVFVVYRYAVATLVIAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           I+FDR                     PVIDQNLY+TGMK TTA F  AMCN++PAF+FLM
Sbjct: 61  IIFDRKARTKMTFSILWKIVLMGLLEPVIDQNLYFTGMKLTTAAFTAAMCNILPAFSFLM 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK +QPSA SGG+A 
Sbjct: 121 AWACRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKGSQPSASSGGSAT 180

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALI+ERG
Sbjct: 181 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIIERG 240

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           NP+AW  HFDSQLLAVVYAGVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 241 NPAAWVFHFDSQLLAVVYAGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 300

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+FLGR++GAV+II GLYLVLWGKSKDQ SVK +CDK+TP EQQM           
Sbjct: 301 ILAEIMFLGRVLGAVAIISGLYLVLWGKSKDQASVKLECDKITPCEQQMAG--------- 360

BLAST of Spg010031 vs. ExPASy TrEMBL
Match: A0A6J1GW81 (WAT1-related protein At2g39510-like OS=Cucurbita moschata OX=3662 GN=LOC111458151 PE=3 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.1e-150
Identity = 288/377 (76.39%), Postives = 317/377 (84.08%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEGF R  GLAKPYL VVF+QFG+AGM+ILAKSALDKGMS +VFVVYR AVATLVI PFA
Sbjct: 65  MEGFARLFGLAKPYLVVVFVQFGYAGMTILAKSALDKGMSPHVFVVYRHAVATLVIAPFA 124

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           ++F+R                     PVIDQNLY+TGMK TTATF  AMCN++PAF+FLM
Sbjct: 125 LIFNRKARTKMTFSILWKIVLLGLLEPVIDQNLYFTGMKLTTATFTAAMCNILPAFSFLM 184

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH 180
           AW CRLEKV+ILKRGSQAKI+GTIVTVGGAMIMTFIRGPMLNLPWT  NQ SA S G+A+
Sbjct: 185 AWACRLEKVNILKRGSQAKIIGTIVTVGGAMIMTFIRGPMLNLPWTNANQHSASSAGSAN 244

Query: 181 HQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERG 240
           HQSP+KGSLMIATGCICW+AFITLQAITLK YP ELSLTALICLVGTIGGSGVALIMERG
Sbjct: 245 HQSPLKGSLMIATGCICWSAFITLQAITLKEYPVELSLTALICLVGTIGGSGVALIMERG 304

Query: 241 NPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSF 300
           N  AW LH DSQLLAVVY+GVIC+GVTYYIQGVVMQTKGPVFVTAF+PLSMILVAIMSSF
Sbjct: 305 NLGAWVLHLDSQLLAVVYSGVICTGVTYYIQGVVMQTKGPVFVTAFSPLSMILVAIMSSF 364

Query: 301 ILSEILFLGRIIGAVSIIIGLYLVLWGKSKDQVSVKSDCDKVTPSEQQMVSGKDEVSKTV 357
           IL+EI+FLGRI+GAV+II GLYLVLWGKSKDQ SV S+CDK+TP EQQM           
Sbjct: 365 ILAEIMFLGRILGAVAIISGLYLVLWGKSKDQTSVDSECDKITPCEQQM----------T 424

BLAST of Spg010031 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 384.0 bits (985), Expect = 1.3e-106
Identity = 203/359 (56.55%), Postives = 258/359 (71.87%), Query Frame = 0

Query: 12  KPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFAIVFDR------ 71
           KP++ VV +QFG+AG+SI+AK AL++GMS +V   YR  VAT+ I PFA   DR      
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 72  ---------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLMAWVCRLEKVSI 131
                          P IDQNLYYTGMK+T+ATF  AM NV+PAFAF+MAW+ RLEKV++
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 132 LKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAHHQSPVKGSLMI 191
            K  SQAKILGTIVTVGGAM+MT ++GP++ LPW  P+     S      Q   KG+ +I
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGASLI 186

Query: 192 ATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERGNPSAWALHFDS 251
           A GCICWA FI LQAITLK+YP ELSLTA IC +G+I  + VAL +ERGNPSAWA+H DS
Sbjct: 187 AIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHLDS 246

Query: 252 QLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSFILSEILFLGRI 311
           +LLA VY GVICSG+ YY+QGV+M+T+GPVFVTAFNPLSM++VAI+ S IL+E++FLGRI
Sbjct: 247 KLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLGRI 306

Query: 312 IGAVSIIIGLYLVLWGKSKDQVSVK-SDCDKVTPSEQQMVSGKDEVSKTVESNEEFVVI 349
           +GA+ I++GLY VLWGKSKD+ S   SD DK  P     +    + +  +++N+  VVI
Sbjct: 307 LGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKELPLSTPQIVLPSKANAKMDTNDASVVI 365

BLAST of Spg010031 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 339.3 bits (869), Expect = 3.6e-93
Identity = 182/353 (51.56%), Postives = 247/353 (69.97%), Query Frame = 0

Query: 11  AKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFAIVFDR----- 70
           A+P++ +V +Q G AGM IL+K+ L+KGMS YV VVYR AVAT+V+ PFA  FD+     
Sbjct: 13  ARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPK 72

Query: 71  ----------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLMAWVCRLEKVS 130
                           PVIDQNLYY GMK+TTATFATAM NV+PA  F++A++  LE+V 
Sbjct: 73  MTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVK 132

Query: 131 ILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAHHQSPVKGSLM 190
           +    S  K++GT+ TVGGAMIMT ++GP+L+L WTK       +G   H  S +KG+++
Sbjct: 133 LRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKGVSAHNTAGTDIH--SAIKGAVL 192

Query: 191 IATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERGNPSAWALHFD 250
           +  GC  +A F+ LQAITL+ YPAELSLTA ICL+GTI G+ VAL+ME+GNPSAWA+ +D
Sbjct: 193 VTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWD 252

Query: 251 SQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSFILSEILFLGR 310
           ++LL   Y+G++CS + YY+ GVVM+T+GPVFVTAF+PL MI+VAIMS+ I +E ++LGR
Sbjct: 253 TKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLGR 312

Query: 311 IIGAVSIIIGLYLVLWGKSKD---QVSVKSDCDKVTPSEQQMVSGKDEVSKTV 340
           ++GAV I  GLYLV+WGK KD     +++ D +   P  +   +GKD V   V
Sbjct: 313 VLGAVVICAGLYLVIWGKGKDYKYNSTLQLDDESAQPKLELSGNGKDNVDHEV 363

BLAST of Spg010031 vs. TAIR 10
Match: AT2G37450.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.7e-88
Identity = 171/322 (53.11%), Postives = 226/322 (70.19%), Query Frame = 0

Query: 11  AKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFAIVFDRPVIDQ 70
           A P++ +V +Q G+AGM IL K  L+KGMS YV  VYR  VAT+V+ PFA  FD PVI Q
Sbjct: 7   ALPFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFDNPVIAQ 66

Query: 71  NLYYTGMKFTTATFATAMCNVVPAFAFLMAWVCRLEKVSILKRGSQAKILGTIVTVGGAM 130
           NL+  GMK+TTATFA A+ N +PA  F++A + RLE V      S AK++GT+ TVGG M
Sbjct: 67  NLFNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVKFQSIRSAAKVVGTVTTVGGIM 126

Query: 131 IMTFIRGPMLNLPWTKPNQPSAPSGGAAHHQSPVKGSLMIATGCICWAAFITLQAITLKA 190
           +MT ++GP L+L WTK   PSA +       S +KG++++  GC  +A F+ LQAITLK 
Sbjct: 127 VMTLVKGPALDLFWTK--GPSAQNTVGTDIHSSIKGAVLVTIGCFSYACFMILQAITLKT 186

Query: 191 YPAELSLTALICLVGTIGGSGVALIMERGNPSAWALHFDSQLLAVVYAGVICSGVTYYIQ 250
           YPAELSL   ICL+GTI G  VAL+ME+GNPS WA+ +D++LL + Y+G++CS + YYI 
Sbjct: 187 YPAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWDTKLLTITYSGIVCSALGYYIG 246

Query: 251 GVVMQTKGPVFVTAFNPLSMILVAIMSSFILSEILFLGRIIGAVSIIIGLYLVLWGKSKD 310
           GVVM+T+GPVFVTAF PL MI+VAIMSS I  E ++LGR +GA  I +GLYLV+WGK+KD
Sbjct: 247 GVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGRALGATVICVGLYLVIWGKAKD 306

Query: 311 -----QVSVKSDCDKVTPSEQQ 328
                   +  D  + T S+Q+
Sbjct: 307 YEYPSTPQIDDDLAQATTSKQK 326

BLAST of Spg010031 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.3e-82
Identity = 161/365 (44.11%), Postives = 245/365 (67.12%), Query Frame = 0

Query: 1   MEGFVRSLGLAKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFA 60
           MEG   ++   +PYL ++F+QFG AG  I+  + L++G ++YV +VYR  VA LV+ PFA
Sbjct: 1   MEGVSATMHKLRPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFA 60

Query: 61  IVFDR---------------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLM 120
           ++F+R                     PV+DQ   Y GM  T+AT+ +A+ N++P+  F++
Sbjct: 61  LIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFII 120

Query: 121 AWVCRLEKVSILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPN--QPSAPSGGA 180
           AW+ R+EKV+I +  S+AKI+GT+V +GGA++MT  +GP++ LPW+ PN  Q +  +  +
Sbjct: 121 AWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNS 180

Query: 181 AHHQSPVKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIME 240
             H + V G+L+I  GC+ W+ F  LQ+IT+K YPA+LSL+ALICL G +    VAL++E
Sbjct: 181 QDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVE 240

Query: 241 RGNPSAWALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMS 300
           R +PS WA+ +D++L A +Y G++ SG+TYY+QG+VM+T+GPVFVTAFNPL MILVA+++
Sbjct: 241 R-HPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIA 300

Query: 301 SFILSEILFLGRIIGAVSIIIGLYLVLWGKSKD-QVSVKSDCDKVTPSEQQMVSGKDEVS 342
           SFIL E +  G +IG   I  GLY+V+WGK KD +VS     +K +  E  + +  ++ +
Sbjct: 301 SFILHEQIHFGCVIGGAVIAAGLYMVVWGKGKDYEVSGLDILEKNSLQELPITTKSEDDN 360

BLAST of Spg010031 vs. TAIR 10
Match: AT5G13670.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.9e-79
Identity = 168/360 (46.67%), Postives = 235/360 (65.28%), Query Frame = 0

Query: 11  AKPYLGVVFMQFGFAGMSILAKSALDKGMSQYVFVVYRQAVATLVITPFAIVFDR----- 70
           A+P++ +VF+Q  +A MSI+AK AL+KGMS +V V YR AVA+ +ITPFA++ +R     
Sbjct: 6   ARPFIAIVFIQCLYALMSIVAKLALNKGMSPHVLVAYRMAVASALITPFALILERNTRPK 65

Query: 71  ----------------PVIDQNLYYTGMKFTTATFATAMCNVVPAFAFLMAWVCRLEKVS 130
                           PV++QNLYY+GMK TTATF +A+CN +PA  F+MA V +LEKV+
Sbjct: 66  LTFKILLQIAILSLFEPVVEQNLYYSGMKLTTATFTSALCNALPAMTFIMACVFKLEKVT 125

Query: 131 ILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNQPSAPSGGAAH------HQSP 190
           I +R SQAK++GT+V +GGAM+MTF++G ++ LPWT     S    G  H          
Sbjct: 126 IERRHSQAKLVGTMVAIGGAMLMTFVKGNVIELPWT---SNSRGLNGHTHAMRIPKQADI 185

Query: 191 VKGSLMIATGCICWAAFITLQAITLKAYPAELSLTALICLVGTIGGSGVALIMERGNPSA 250
            +GS+M+   C  W+ +I LQA  L  Y AELSLTAL+C++G +  + + LI ER N S 
Sbjct: 186 ARGSIMLVASCFSWSCYIILQAKILAQYKAELSLTALMCIMGMLEATVMGLIWERKNMSV 245

Query: 251 WALHFDSQLLAVVYAGVICSGVTYYIQGVVMQTKGPVFVTAFNPLSMILVAIMSSFILSE 310
           W ++ D  LLA +Y G++ SG+ YY+ G   + +GPVFV+AFNPLSM+LVAI+S+F+  E
Sbjct: 246 WKINPDVTLLASIYGGLV-SGLAYYVIGWASKERGPVFVSAFNPLSMVLVAILSTFVFLE 305

Query: 311 ILFLGRIIGAVSIIIGLYLVLWGKSKD------------QVSVKSDCDKV-TPSEQQMVS 331
            +++GR+IG+V I+IG+YLVLWGKSKD            +  VK D  KV TP   Q+VS
Sbjct: 306 KVYVGRVIGSVVIVIGIYLVLWGKSKDKGGMLQPNAGCAETVVKIDQQKVPTPDNNQVVS 361