Spg010015 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg010015 vs. NCBI nr
Match: XP_022979186.1 (cucumisin-like [Cucurbita maxima])

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 671/742 (90.43%), Postives = 709/742 (95.55%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLD-SDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV 65
           M SSL  KLV        L+ASSLD SD+DGRK+YIVYLGNKPED+ASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 66  VGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMG 125
           VGSTFAP++LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 126 FTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKII 185
           FTK+V RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKG CQ SANF CNRKII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 186 GARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 245
           GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 246 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGI 305
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+KPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 306 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGK 365
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVS+VQLGN+N+YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 366 QYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGV 425
           QYPLIYAGNAPN+SGGFTGSSSRFCS+NSVDRNLVRGKILLCDSIL+P+TFASF+GAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 426 IMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSF 485
           +MNDAGVKDN+RSYPLPSSYLG  AGNN+KTYM SN FPTATIFKSNAVNDT+APL+VSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 486 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHAT 545
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV DSR TLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 546 AAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDA 605
           AAAVYVKTFHPSWSPAAIKSALMTTA PLN KLNPQAEFAYGAGHINP+KAV+PGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 606 SESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 665
            ESDYVRFLCGQGYTTAMVRRLSGD+SVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 666 RTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSAS 725
           RT+TNVGSKV+ YRAK+LGAP+GLTI+VNPP LSFNAIGQKKSFT+TVRG V+Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 726 LVWSDGYHNVRSPITVYVVVKA 747
           L+W+DG+H+VRSPITVYVV KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of Spg010015 vs. NCBI nr
Match: KAG6600369.1 (hypothetical protein SDJN03_05602, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1353.6 bits (3502), Expect = 0.0e+00
Identity = 670/742 (90.30%), Postives = 707/742 (95.28%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLD-SDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV 65
           M SSL  KLV        L+ASSLD SD+DGRK+YIVYLGNKPED+ASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGCLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 66  VGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMG 125
           VGSTFAP++LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 126 FTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKII 185
           FTKNV RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKG CQ SANF CNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 186 GARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 245
           GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 246 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGI 305
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+KPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 306 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGK 365
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVS+VQLGN+N+YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 366 QYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGV 425
           QYPLIYAGNAPN+SGGFTGSSSRFCS+NSVDRNLVRGKILLCDSIL+P+TFASF+GAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 426 IMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSF 485
           +MNDAGVKDNAR+YPLPSSYLG  AGNN+KTYM SN FPTATIFKSNAVNDT+APL+VSF
Sbjct: 421 VMNDAGVKDNARTYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 486 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHAT 545
           SSRGPNPETYDILKPDLTAPGVEILAAWSPI SVSSGV DSR TLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIVSVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 546 AAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDA 605
           AAAVYVKTFHPSWSPAAIKSALMTTA PLN KLNPQAEFAYGAGHINP+KAV+PGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 606 SESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 665
            ESDYVRFLCGQGYTTAMVRRLSGD+SVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 666 RTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSAS 725
           RT+TNVGSKV+ YRAK+LG P+GLTI+VNPPVLSFNAIGQKKSFT+TVRG V+Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 726 LVWSDGYHNVRSPITVYVVVKA 747
           L+W+DG+H+VRSPITVYVV KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of Spg010015 vs. NCBI nr
Match: XP_023536304.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 671/742 (90.43%), Postives = 707/742 (95.28%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLD-SDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV 65
           M SSL  KLV        L+ASSLD SD+DGRK+YIVYLGNKPED+ASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 66  VGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMG 125
           VGSTFAP++LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 126 FTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKII 185
           FTKNV RVKQVESNIVVGVLDSGIWPESPSFSDVGYG PPAKWKG CQ SANF CNRKI+
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIV 180

Query: 186 GARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 245
           GARAYRSD  FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 246 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGI 305
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+KPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 306 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGK 365
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVS+VQLGN+N+YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 366 QYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGV 425
           QYPLIYAGNAPN+SGGFTGSSSRFCS+NSVDRNLVRGKILLCDSIL+P+TFASFSGAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGV 420

Query: 426 IMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSF 485
           +MNDAGVKDNARSYPLPSSYLG  AGNN+KTYM SN FPTATIFKSN VNDT+APL+VSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSF 480

Query: 486 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHAT 545
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSR TLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHAT 540

Query: 546 AAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDA 605
           AAAVYVKTFHPSWSPAAIKSALMTTA PLN KLNPQAEFAYGAGHINP+KAV+PGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 606 SESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 665
            ESDYVRFLCGQGYTTAMVRRLSGD+SVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 666 RTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSAS 725
           RT+TNVGSKV+ YRAK+LG P+GLTI+VNPPVLSFNAIGQKKSFT+TVRG V+Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 726 LVWSDGYHNVRSPITVYVVVKA 747
           L+W+DG+H+VRSPITVYVV KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of Spg010015 vs. NCBI nr
Match: XP_022941960.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 670/742 (90.30%), Postives = 707/742 (95.28%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLD-SDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV 65
           M SSL  KLV        L+AS LD SD+DGRK+YIVYLGNKPED+ASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 66  VGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMG 125
           VGSTFAP++LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 126 FTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKII 185
           FTKNV RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKG CQ SANF CNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 186 GARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 245
           GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 246 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGI 305
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+KPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 306 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGK 365
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVS+VQLGN+N+YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 366 QYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGV 425
           QYPLIYAGNAPN+SGGFTGSSSRFCS+NSVDRNLVRGKILLCDSIL+P+TFASF+GAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 426 IMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSF 485
           +MNDAGVKDNARSYPLPSSYLG  AGNN+KTYM S+ FPTATIFKSNAVNDT+APL+VSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 486 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHAT 545
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV DSR TLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 546 AAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDA 605
           AAAVYVKTFHPSWSPAAIKSALMTTA PLN KLNPQAEFAYGAGHINP+KAV+PGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 606 SESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 665
            ESDYVRFLCGQGYTTAMVRRLSGD+SVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 666 RTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSAS 725
           RT+TNVGSKV+ YRAK+LG P+GLTI+VNPPVLSFNAIGQKKSFT+TVRG V+Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 726 LVWSDGYHNVRSPITVYVVVKA 747
           L+W+DG+H+VRSPITVYVV KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of Spg010015 vs. NCBI nr
Match: XP_038893252.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 1350.1 bits (3493), Expect = 0.0e+00
Identity = 673/752 (89.49%), Postives = 705/752 (93.75%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLDSDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEVV 65
           M SSL  KLV L  L + L+ASSLDSD+DGRKIYIVYLGNKPED+ASTPSHHMRMLEEVV
Sbjct: 1   MSSSLIFKLVVLFGLFTSLLASSLDSDNDGRKIYIVYLGNKPEDSASTPSHHMRMLEEVV 60

Query: 66  GSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGF 125
           GSTFAP++LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGF
Sbjct: 61  GSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGF 120

Query: 126 TKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIG 185
           TKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKG CQ SANF CNRKIIG
Sbjct: 121 TKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIG 180

Query: 186 ARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 245
           ARAYR DNFFPP DI+SPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA
Sbjct: 181 ARAYRGDNFFPPGDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 240

Query: 246 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGIL 305
           VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+KPKYYFNDSIAIGAFHSMKHGIL
Sbjct: 241 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGIL 300

Query: 306 TSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQ 365
           TSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVS VQLGN+NIYQGYTINTFDL GKQ
Sbjct: 301 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSNVQLGNKNIYQGYTINTFDLKGKQ 360

Query: 366 YPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVI 425
           YPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLV+GKI+LCDS+LAPATFAS +GA+GVI
Sbjct: 361 YPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVKGKIVLCDSVLAPATFASLNGAMGVI 420

Query: 426 MNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSN-------------NFPTATIFKSNA 485
           MNDAGVKDNARSYPLPSSYLGT AG+NVKTYM  N              FPTATIFKSNA
Sbjct: 421 MNDAGVKDNARSYPLPSSYLGTVAGDNVKTYMDQNKYVSTTRYHFSIDRFPTATIFKSNA 480

Query: 486 VNDTTAPLVVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNI 545
           VNDT+AP +VSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGVRD R TLYNI
Sbjct: 481 VNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDPRTTLYNI 540

Query: 546 ISGTSMSCPHATAAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINP 605
           ISGTSMSCPHATAAAVYVKTFHP+WSPAAIKSALMTTATP+NAKLNPQ EFAYGAGHINP
Sbjct: 541 ISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPMNAKLNPQVEFAYGAGHINP 600

Query: 606 LKAVHPGLVYDASESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALS 665
           LKAVHPGL+YDA ESDYVRFLCGQGYTTAMVRRLSGDNS C+RANSGR+WDLNYPSFALS
Sbjct: 601 LKAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSACSRANSGRIWDLNYPSFALS 660

Query: 666 STPSESINQFFRRTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTV 725
           ST   S NQFFRRT+TNVGSKVS YRAK++GAP+GLTITVNP VLSFNAIGQKKSFTLT+
Sbjct: 661 STSPHSFNQFFRRTVTNVGSKVSTYRAKVVGAPRGLTITVNPSVLSFNAIGQKKSFTLTI 720

Query: 726 RGSVSQSIVSASLVWSDGYHNVRSPITVYVVV 745
           RGSVSQSIVSASLVWSDGYHNVRSPIT+  +V
Sbjct: 721 RGSVSQSIVSASLVWSDGYHNVRSPITITSLV 752

BLAST of Spg010015 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 5.9e-276
Identity = 482/740 (65.14%), Postives = 576/740 (77.84%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLDSDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEVV 65
           M SSL  KL F +L  S  +AS LDSDDDG+ IYIVY+G K ED  S   HH  MLE+VV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 66  GSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGF 125
           GSTFAPES+LH+YKRSFNGF VKLTEEEA+KI++ EGVVSVF NE   LHTTRSWDF+GF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 126 TKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIG 185
              VPR  QVESNIVVGVLD+GIWPESPSF D G+ PPP KWKGTC+ S NF CNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 186 ARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 245
           AR+Y       P D+  PRD++GHGTHTAST AGGLV+QA+LYGL LGTARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 246 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGIL 305
            YK+CW+DGC D DILAA+DDAIADGVDIISLSVGG+ P++YF D+IAIG+FH+++ GIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 306 TSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQ 365
           TSNSAGN GP++FT  + SPW LSVAASTMDRK V++VQ+GN   +QG +INTFD   + 
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQY 360

Query: 366 YPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAP-ATFASFSGAVGV 425
           YPL+   + PN   GF  S+SRFC+  SV+ NL++GKI++C++   P   F S  GA GV
Sbjct: 361 YPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGV 420

Query: 426 IMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSF 485
           +M  +  +D A SYPLPSS L          Y+ S   P ATIFKS  + + +AP+VVSF
Sbjct: 421 LMT-SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSF 480

Query: 486 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHAT 545
           SSRGPN  T D++KPD++ PGVEILAAW  +A V  G+R  R TL+NIISGTSMSCPH T
Sbjct: 481 SSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGIR--RNTLFNIISGTSMSCPHIT 540

Query: 546 AAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDA 605
             A YVKT++P+WSPAAIKSALMTTA+P+NA+ NPQAEFAYG+GH+NPLKAV PGLVYDA
Sbjct: 541 GIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDA 600

Query: 606 SESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 665
           +ESDYV+FLCGQGY T  VRR++GD S CT  N+GRVWDLNYPSF LS +PS++ NQ+F 
Sbjct: 601 NESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFN 660

Query: 666 RTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSAS 725
           RTLT+V  + S YRA ++ APQGLTI+VNP VLSFN +G +KSFTLTVRGS+   +VSAS
Sbjct: 661 RTLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSAS 720

Query: 726 LVWSDGYHNVRSPITVYVVV 745
           LVWSDG H VRSPIT+  +V
Sbjct: 721 LVWSDGVHYVRSPITITSLV 731

BLAST of Spg010015 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 7.4e-194
Identity = 354/734 (48.23%), Postives = 479/734 (65.26%), Query Frame = 0

Query: 17  LNLLCSLLVASSLDSDDDGRK---IYIVYLGNKPEDTASTPSHHMRMLEEVVGSTFAPES 76
           L L+C   + +   S +D R+   +YIVY+G  PE   S PSHH+ +L+++VG+  A   
Sbjct: 8   LYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHL 67

Query: 77  LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGFTKNVPRVK 136
           L+ SYKRSFNGF   L++ E+QK+   + VVSVFP++   L TTRSWDF+GF +   R  
Sbjct: 68  LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRES 127

Query: 137 QVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGARAYRSDN 196
             ES+++VGV+DSGIWPES SF D G+GPPP KWKG+C+    F CN K+IGAR Y    
Sbjct: 128 VKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYN--- 187

Query: 197 FFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWSD 256
               +   S RD +GHGTHTAST AG  V  AS YGLA GTARGGVPSARIA YK+C+ +
Sbjct: 188 ----KFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-N 247

Query: 257 GCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTSNSAGND 316
            C D DILAAFDDAIADGVD+IS+S+         N S+AIG+FH+M  GI+T+ SAGN+
Sbjct: 248 RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNN 307

Query: 317 GPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYPLIYAGN 376
           GPD  ++ N SPW ++VAAS  DR+ + RV LGN     G ++NTF+L G ++P++Y   
Sbjct: 308 GPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY--- 367

Query: 377 APNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMNDAGVKD 436
             N+S   + + + +CS   VD  LV+GKI+LCD  L     A  +GA+GVI+ +  + D
Sbjct: 368 GQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPD 427

Query: 437 NARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSRGPNPET 496
           +A   P P+S LG     ++K+Y+ S   P A I ++  + D  AP V SFSSRGP+   
Sbjct: 428 SAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVI 487

Query: 497 YDILKPDLTAPGVEILAAWSPIASVSS--GVRDSRKTLYNIISGTSMSCPHATAAAVYVK 556
            ++LKPD++APG+EILAA+SP+AS SS     D R   Y+++SGTSM+CPH    A YVK
Sbjct: 488 QNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVK 547

Query: 557 TFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDASESDYVR 616
           +FHP WSP+AIKSA+MTTATP+N K NP+ EFAYG+G INP KA  PGLVY+    DY++
Sbjct: 548 SFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLK 607

Query: 617 FLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTLTNVG 676
            LC +G+ +  +   SG N  C+      V DLNYP+     +  +  N  F+RT+TNVG
Sbjct: 608 MLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVG 667

Query: 677 SKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQ--SIVSASLVWSD 736
              S Y+A ++     L I++ P +L F  + +KKSF +T+ G   +  S VS+S+VWSD
Sbjct: 668 FPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSD 727

Query: 737 GYHNVRSPITVYVV 744
           G H+VRSPI  Y +
Sbjct: 728 GSHSVRSPIVAYSI 727

BLAST of Spg010015 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 1.4e-184
Identity = 352/738 (47.70%), Postives = 477/738 (64.63%), Query Frame = 0

Query: 17  LNLLCSLLVAS--SLDSDDDGRKIYIVYLGNKPEDTASTP-SHHMRMLEEVVGSTFAPES 76
           L+ + +LLV S  S   DD  +++YIVY+G  P      P SHH  +L++V G +   + 
Sbjct: 10  LSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQDR 69

Query: 77  LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGFT--KNVPR 136
           L+ +YKRSFNGF  +LTE E + +++ + VVSVFP++  +L TT SW+FMG    K   R
Sbjct: 70  LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 129

Query: 137 VKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGARAYRS 196
              +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+   NF CN K+IGAR Y  
Sbjct: 130 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTP 189

Query: 197 DNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICW 256
                PE   S RD+ GHG+HTAS  AG  V   S YGL  GT RGGVP+ARIAVYK+C 
Sbjct: 190 KLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCD 249

Query: 257 SD--GCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTSNS 316
                C    ILAAFDDAIAD VDII++S+G      +  D++AIGAFH+M  GILT N 
Sbjct: 250 PGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNG 309

Query: 317 AGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYPLI 376
           AGN+GP+  TI + +PW  +VAAS M+R  +++V LGN     G ++N+FDL GK+YPL+
Sbjct: 310 AGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLV 369

Query: 377 YAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMNDA 436
           Y  +A   S     SS+ FCS   +D   V+GKI+LCD+   P    +      ++ N  
Sbjct: 370 YGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNP- 429

Query: 437 GVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSRGP 496
             +D A  +  P S L     N V +Y++S   P A + KS  + +  AP+V S+SSRGP
Sbjct: 430 -YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGP 489

Query: 497 NPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHATAAAVY 556
           NP  +DILKPD+TAPG EILAA+SP    S    D+R   Y +ISGTSMSCPH    A Y
Sbjct: 490 NPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTVISGTSMSCPHVAGVAAY 549

Query: 557 VKTFHPSWSPAAIKSALMTTATPLNAKLNPQ---AEFAYGAGHINPLKAVHPGLVYDASE 616
           +KTFHP WSP+ I+SA+MTTA P+NA  +P    AEFAYGAGH++P+ A+HPGLVY+A++
Sbjct: 550 IKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANK 609

Query: 617 SDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW-DLNYPSFALSSTPSESINQFFRR 676
           SD++ FLCG  YT   +R +SGD+S CT+  +  +  +LNYPS +   + ++     FRR
Sbjct: 610 SDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRR 669

Query: 677 TLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGS--VSQSIVSA 736
           T+TNVG   + Y+AK++G+   L + V P VLS  ++ +KKSFT+TV G+   ++++VSA
Sbjct: 670 TVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSA 729

Query: 737 SLVWSDGYHNVRSPITVY 742
            L+WSDG H VRSPI VY
Sbjct: 730 QLIWSDGVHFVRSPIVVY 735

BLAST of Spg010015 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 2.4e-184
Identity = 351/742 (47.30%), Postives = 488/742 (65.77%), Query Frame = 0

Query: 16  FLNLLCSLLVAS----SLDSDDDG-RKIYIVYLGNKPEDTASTP-SHHMRMLEEVVGSTF 75
           F+ L  SLLV S    S D DD G +++YIVYLG+ P     TP S HM +L+E+ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 76  APESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGFTKNV 135
               L+ SYK+SFNGF  +LTE E ++++  E VVSVFP+ K  L TT SW+FMG  + +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 136 --PRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGAR 195
              R + +ES+ ++GV+DSGI+PES SFSD G+GPPP KWKGTC    NF CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186

Query: 196 AYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVY 255
            Y + +    +  ++ RD  GHGTHTAS  AG  V  ++ YGL  GTARGGVP+ARIAVY
Sbjct: 187 DYTAKS----KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVY 246

Query: 256 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTS 315
           K+C ++GC    +++AFDDAIADGVD+IS+S+       +  D IAIGAFH+M  G+LT 
Sbjct: 247 KVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTV 306

Query: 316 NSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYP 375
           N+AGN+GP   T+ + +PW  SVAAS  +R  +++V LG+  I  G ++NT+D+ G  YP
Sbjct: 307 NAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYP 366

Query: 376 LIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMN 435
           L+Y  +A       +   +R C    +D  LV+GKI+LCDS       A   GAVG I+ 
Sbjct: 367 LVYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKG-LIEAQKLGAVGSIVK 426

Query: 436 DAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSR 495
           +    D A     P S+L      ++ +YM+S   P AT+ KS  +++  APLV SFSSR
Sbjct: 427 NP-EPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSR 486

Query: 496 GPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHATAAA 555
           GP+    DILKPD+TAPGVEILAA+SP +S +    D+R+  Y+++SGTSM+CPH    A
Sbjct: 487 GPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVA 546

Query: 556 VYVKTFHPSWSPAAIKSALMTTATPLNAKLN--PQAEFAYGAGHINPLKAVHPGLVYDAS 615
            YVKTFHP WSP+ I+SA+MTTA P+NA  +     EFAYG+GH++P+ A++PGLVY+ +
Sbjct: 547 AYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELT 606

Query: 616 ESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFR 675
           ++D++ FLCG  YT+  +R +SGDNS CT+  S  +  +LNYP+ +   + ++  N  F+
Sbjct: 607 KADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQ 666

Query: 676 RTLTNVGSKVSIYRAKILGAP-QGLTITVNPPVLSFNAIGQKKSFTLTVRGSV--SQSIV 735
           RT+TNVG + S Y AK++  P   L+I V+P VLS  ++ +K+SF +TV      ++  V
Sbjct: 667 RTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPV 726

Query: 736 SASLVWSDGYHNVRSPITVYVV 744
           SA+L+WSDG HNVRSPI VY +
Sbjct: 727 SANLIWSDGTHNVRSPIIVYAM 740

BLAST of Spg010015 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 3.8e-182
Identity = 349/735 (47.48%), Postives = 468/735 (63.67%), Query Frame = 0

Query: 18  NLLCSLLV--ASSLDSDDDGRKIYIVYLGNKPEDTASTP-SHHMRMLEEVVGSTFAPESL 77
           +LL  LLV   SS+ +  D +++YIVY+G+       TP S HM +L+EV G +     L
Sbjct: 9   SLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRL 68

Query: 78  LHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGFTKNV--PRV 137
           + SYKRSFNGF  +LTE E ++++   GVVSVFPN+K  L TT SWDFMG  + +   R 
Sbjct: 69  VRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRN 128

Query: 138 KQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGARAYRSD 197
             VES+ ++GV+DSGI PES SFSD G+GPPP KWKG C    NF CN K+IGAR Y S+
Sbjct: 129 PTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE 188

Query: 198 NFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWS 257
                      RD DGHGTHTAST AG  V  AS +G+  GT RGGVP++R+A YK+C  
Sbjct: 189 ---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTP 248

Query: 258 DGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTSNSAGN 317
            GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH+M  G+LT NSAGN
Sbjct: 249 TGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGN 308

Query: 318 DGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYPLIYAG 377
            GP   ++   +PW L+VAAST +R  V++V LGN     G ++N +++ GK YPL+Y  
Sbjct: 309 SGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGK 368

Query: 378 NAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMNDAGVK 437
           +A   S      S+  C  + VD++ V+GKIL+C             GAVG+I       
Sbjct: 369 SA--ASSACDAESAGLCELSCVDKSRVKGKILVCGG-PGGLKIVESVGAVGLIYR-TPKP 428

Query: 438 DNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSRGPNPE 497
           D A  +PLP++ L T    ++ +Y+ S + P A + K+ A+ + T+P++ SFSSRGPN  
Sbjct: 429 DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTI 488

Query: 498 TYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHATAAAVYVKT 557
             DILKPD+TAPGVEILAA+SP    S    D+R   Y+++SGTSMSCPH    A YVKT
Sbjct: 489 AVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKT 548

Query: 558 FHPSWSPAAIKSALMTTATPLNAKLN--PQAEFAYGAGHINPLKAVHPGLVYDASESDYV 617
           F+P WSP+ I+SA+MTTA P+NA        EFAYG+GH++P+ A +PGLVY+  +SD++
Sbjct: 549 FNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHI 608

Query: 618 RFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSF-ALSSTPSESINQFFRRTLTN 677
            FLCG  YT+ +++ +SG+   C+ A      +LNYPS  A  S    +    F RTLTN
Sbjct: 609 AFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTN 668

Query: 678 VGSKVSIYRAKIL-GAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIV--SASLV 737
           VG+  S Y +K++ G    L + + P VLSF  + +K+SFT+TV GS   S V  SA+L+
Sbjct: 669 VGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLI 728

Query: 738 WSDGYHNVRSPITVY 742
           WSDG HNVRSPI VY
Sbjct: 729 WSDGTHNVRSPIVVY 728

BLAST of Spg010015 vs. ExPASy TrEMBL
Match: A0A6J1IQ27 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1)

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 671/742 (90.43%), Postives = 709/742 (95.55%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLD-SDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV 65
           M SSL  KLV        L+ASSLD SD+DGRK+YIVYLGNKPED+ASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 66  VGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMG 125
           VGSTFAP++LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 126 FTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKII 185
           FTK+V RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKG CQ SANF CNRKII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 186 GARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 245
           GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 246 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGI 305
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+KPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 306 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGK 365
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVS+VQLGN+N+YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 366 QYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGV 425
           QYPLIYAGNAPN+SGGFTGSSSRFCS+NSVDRNLVRGKILLCDSIL+P+TFASF+GAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 426 IMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSF 485
           +MNDAGVKDN+RSYPLPSSYLG  AGNN+KTYM SN FPTATIFKSNAVNDT+APL+VSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 486 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHAT 545
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV DSR TLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 546 AAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDA 605
           AAAVYVKTFHPSWSPAAIKSALMTTA PLN KLNPQAEFAYGAGHINP+KAV+PGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 606 SESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 665
            ESDYVRFLCGQGYTTAMVRRLSGD+SVCTRANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 666 RTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSAS 725
           RT+TNVGSKV+ YRAK+LGAP+GLTI+VNPP LSFNAIGQKKSFT+TVRG V+Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 726 LVWSDGYHNVRSPITVYVVVKA 747
           L+W+DG+H+VRSPITVYVV KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of Spg010015 vs. ExPASy TrEMBL
Match: A0A6J1FV97 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1)

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 670/742 (90.30%), Postives = 707/742 (95.28%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLD-SDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV 65
           M SSL  KLV        L+AS LD SD+DGRK+YIVYLGNKPED+ASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 66  VGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMG 125
           VGSTFAP++LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 126 FTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKII 185
           FTKNV RVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKG CQ SANF CNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 186 GARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 245
           GARAYRSDN FPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 246 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGI 305
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+KPKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 306 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGK 365
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVS+VQLGN+N+YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 366 QYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGV 425
           QYPLIYAGNAPN+SGGFTGSSSRFCS+NSVDRNLVRGKILLCDSIL+P+TFASF+GAVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 426 IMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSF 485
           +MNDAGVKDNARSYPLPSSYLG  AGNN+KTYM S+ FPTATIFKSNAVNDT+APL+VSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 486 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHAT 545
           SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGV DSR TLYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 546 AAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDA 605
           AAAVYVKTFHPSWSPAAIKSALMTTA PLN KLNPQAEFAYGAGHINP+KAV+PGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 606 SESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 665
            ESDYVRFLCGQGYTTAMVRRLSGD+SVCT ANSGRVWDLNYPSFALSSTPSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 666 RTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSAS 725
           RT+TNVGSKV+ YRAK+LG P+GLTI+VNPPVLSFNAIGQKKSFT+TVRG V+Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 726 LVWSDGYHNVRSPITVYVVVKA 747
           L+W+DG+H+VRSPITVYVV KA
Sbjct: 721 LLWTDGHHHVRSPITVYVVDKA 742

BLAST of Spg010015 vs. ExPASy TrEMBL
Match: A0A1S3CFE1 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=3 SV=1)

HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 663/742 (89.35%), Postives = 704/742 (94.88%), Query Frame = 0

Query: 3   RSIMPSSL-FSKLVFLNLLCSLLVASSLDSDDDGRKIYIVYLGNKPEDTASTPSHHMRML 62
           RS+  SSL F  +VF +L CSLL AS LDSD+DGRKIYIVY+GNKPED+ASTPSHHMRML
Sbjct: 11  RSMSSSSLVFKFVVFSSLFCSLL-ASGLDSDNDGRKIYIVYMGNKPEDSASTPSHHMRML 70

Query: 63  EEVVGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWD 122
           EEVVGS+FAPE+LLHSYKRSFNGFVVKLTEEEAQKISAKE VVSVFPNEKKHLHTTRSWD
Sbjct: 71  EEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWD 130

Query: 123 FMGFTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNR 182
           FMGFT+NVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKG CQ SANF CNR
Sbjct: 131 FMGFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNR 190

Query: 183 KIIGARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPS 242
           KIIGARAYRSD FFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPS
Sbjct: 191 KIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPS 250

Query: 243 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMK 302
           ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG++PKYYFNDSIAIGAFHSMK
Sbjct: 251 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMK 310

Query: 303 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDL 362
           HGILTSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVSRVQLGN+NIYQGYTINTFDL
Sbjct: 311 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDL 370

Query: 363 LGKQYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGA 422
            GKQYPLIYAG+APNISGGFTGSSSRFCS+NSVDRNLV+GKI+LCDS+L+PATF S +GA
Sbjct: 371 KGKQYPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGA 430

Query: 423 VGVIMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLV 482
           +GV+MND GVKDNARSYPLPSSYL    GNN+KTYM  N FPTATI KSNAVNDT+AP +
Sbjct: 431 MGVVMNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWI 490

Query: 483 VSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCP 542
           VSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGVRDSR TLYNIISGTSMSCP
Sbjct: 491 VSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCP 550

Query: 543 HATAAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLV 602
           HATAAAVYVKTFHP+WSPAAIKSALMTTATPLNAKLN Q EFAYGAGHINPLKAVHPGL+
Sbjct: 551 HATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLL 610

Query: 603 YDASESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQ 662
           YDA E DYVRFLCGQGYTTAMVRRLSGDNS C+RANSGRVWDLNYPSFALSST ++S NQ
Sbjct: 611 YDAYERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQ 670

Query: 663 FFRRTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIV 722
           FFRRT+TNVGSKVS YRAK++GAP+GL+ITVNPPVLSFNAIGQKKSFTLT+RGS+ QSIV
Sbjct: 671 FFRRTVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIV 730

Query: 723 SASLVWSDGYHNVRSPITVYVV 744
           SASL+WSDGYHNVRSPITV+VV
Sbjct: 731 SASLMWSDGYHNVRSPITVFVV 751

BLAST of Spg010015 vs. ExPASy TrEMBL
Match: A0A5A7UBK2 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001150 PE=3 SV=1)

HSP 1 Score: 1339.3 bits (3465), Expect = 0.0e+00
Identity = 659/736 (89.54%), Postives = 700/736 (95.11%), Query Frame = 0

Query: 8   SSLFSKLVFLNLLCSLLVASSLDSDDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEVVGS 67
           S +F  +VF +L CSLL AS LDSD+DGRKIYIVY+GNKPED+ASTPSHHMRMLEEVVGS
Sbjct: 5   SLVFKFVVFSSLFCSLL-ASGLDSDNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVVGS 64

Query: 68  TFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGFTK 127
           +FAPE+LLHSYKRSFNGFVVKLTEEEAQKISAKE VVSVFPNEKKHLHTTRSWDFMGFT+
Sbjct: 65  SFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGFTQ 124

Query: 128 NVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGAR 187
           NVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKG CQ SANF CNRKIIGAR
Sbjct: 125 NVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGAR 184

Query: 188 AYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVY 247
           AYRSD FFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSARIAVY
Sbjct: 185 AYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIAVY 244

Query: 248 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTS 307
           KICWSDGCYDADILAAFDDAIADGVDIISLSVGG++PKYYFNDSIAIGAFHSMKHGILTS
Sbjct: 245 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGILTS 304

Query: 308 NSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYP 367
           NSAGNDGPDYFTIRNFSPWSLSVAAS++DRKLVSRVQLGN+NIYQGYTINTFDL GKQYP
Sbjct: 305 NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQYP 364

Query: 368 LIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMN 427
           LIYAG+APNISGGFTGSSSRFCS+NSVDRNLV+GKI+LCDS+L+PATF S +GA+GV+MN
Sbjct: 365 LIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMGVVMN 424

Query: 428 DAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSR 487
           D GVKDNARSYPLPSSYL    GNN+KTYM  N FPTATI KSNAVNDT+AP +VSFSSR
Sbjct: 425 DLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFSSR 484

Query: 488 GPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHATAAA 547
           GPNPETYDILKPDLTAPGVEILAAWSPIA+VSSGVRDSR TLYNIISGTSMSCPHATAAA
Sbjct: 485 GPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATAAA 544

Query: 548 VYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDASES 607
           VYVKTFHP+WSPAAIKSALMTTATPLNAKLN Q EFAYGAGHINPLKAVHPGL+YDA E 
Sbjct: 545 VYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAYER 604

Query: 608 DYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRTL 667
           DYVRFLCGQGYTTAMVRRLSGDNS C+RANSGRVWDLNYPSFALSST ++S NQFFRRT+
Sbjct: 605 DYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRRTV 664

Query: 668 TNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSASLVW 727
           TNVGSKVS YRAK++GAP+GL+ITVNPPVLSFNAIGQKKSFTLT+RGS+ QSIVSASL+W
Sbjct: 665 TNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASLMW 724

Query: 728 SDGYHNVRSPITVYVV 744
           SDGYHNVRSPITV+VV
Sbjct: 725 SDGYHNVRSPITVFVV 739

BLAST of Spg010015 vs. ExPASy TrEMBL
Match: A0A6J1CEK6 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1)

HSP 1 Score: 1301.2 bits (3366), Expect = 0.0e+00
Identity = 644/741 (86.91%), Postives = 695/741 (93.79%), Query Frame = 0

Query: 6   MPSSLFSKLVFLNLLCSLLVASSLDS-DDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV 65
           M SSL  KLVFL+LLC LL ASSLDS +DDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVFLSLLCGLL-ASSLDSNNDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEV 60

Query: 66  VGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMG 125
           VGSTFAPE+LLHSYKRSFNGFVVKLTEEEAQKI+AKEGVVSVF N KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPEALLHSYKRSFNGFVVKLTEEEAQKIAAKEGVVSVFLNGKKHLHTTRSWDFMG 120

Query: 126 FTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKII 185
           FTKNV RVKQVESNIVVGVLDSGIWPESPSFSD+GYGPPP KWKGTCQ SANFHCN+KII
Sbjct: 121 FTKNVARVKQVESNIVVGVLDSGIWPESPSFSDLGYGPPPVKWKGTCQTSANFHCNKKII 180

Query: 186 GARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 245
           GARAYRS+NFFPPEDI+SPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI
Sbjct: 181 GARAYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 246 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGI 305
           AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS+PKYYFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGI 300

Query: 306 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGK 365
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS++DRK V++VQLGN+N+YQGYTINTFDLLGK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLYQGYTINTFDLLGK 360

Query: 366 QYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGV 425
           QYPLIY G+APNI+GGFT SSSRFCSKN+VDRNLV+GKIL+CD++L+P+ FASF GAVGV
Sbjct: 361 QYPLIYGGDAPNITGGFTSSSSRFCSKNTVDRNLVKGKILVCDTVLSPSAFASFXGAVGV 420

Query: 426 IMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSF 485
           +M DAGVKDNARSYPLP+SYLGT AG ++K YM+SN   TATI KSNAVNDT+APLVVSF
Sbjct: 421 VMQDAGVKDNARSYPLPASYLGTAAGGSIKNYMASNRAATATILKSNAVNDTSAPLVVSF 480

Query: 486 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHAT 545
           SSRGPNPET+DILKPDLTAPGVEILAAWSPIA + SG+ DSRK+LYNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETFDILKPDLTAPGVEILAAWSPIAPI-SGITDSRKSLYNIISGTSMSCPHAT 540

Query: 546 AAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQAEFAYGAGHINPLKAVHPGLVYDA 605
           A AVYVKTFHP+WSPAAIKSALMTTATPLN KLNPQAEFAYGAGH+NPLKAV+PGLVYDA
Sbjct: 541 AVAVYVKTFHPTWSPAAIKSALMTTATPLNPKLNPQAEFAYGAGHVNPLKAVNPGLVYDA 600

Query: 606 SESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 665
            ESDYV+FLCGQGYTTAMV+RL+ D S CT AN+ RVWDLNYPSFALSSTPSESINQFF 
Sbjct: 601 HESDYVKFLCGQGYTTAMVQRLTADASACTPANTARVWDLNYPSFALSSTPSESINQFFT 660

Query: 666 RTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIVSAS 725
           RTLTNVGSK S Y+A + GAP GLTI+V+PPVLSF+AIG+KKSF LT+RG++S SIVSAS
Sbjct: 661 RTLTNVGSKASTYKAAVYGAPPGLTISVSPPVLSFHAIGEKKSFKLTIRGTLSHSIVSAS 720

Query: 726 LVWSDGYHNVRSPITVYVVVK 746
           LVW+DG H VRSPITVYVV K
Sbjct: 721 LVWNDGLHQVRSPITVYVVNK 739

BLAST of Spg010015 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 667.5 bits (1721), Expect = 1.2e-191
Identity = 344/705 (48.79%), Postives = 463/705 (65.67%), Query Frame = 0

Query: 43  LGNKPEDTASTPSHHMRMLEEVVGSTFAPESLLHSYKRSFNGFVVKLTEEEAQKISAKEG 102
           +G  PE   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF   L++ E+QK+   + 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 103 VVSVFPNEKKHLHTTRSWDFMGFTKNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 162
           VVSVFP++   L TTRSWDF+GF +   R    ES+++VGV+DSGIWPES SF D G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 163 PPAKWKGTCQASANFHCNRKIIGARAYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLV 222
           PP KWKG+C+    F CN K+IGAR Y        +   S RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTAAGNAV 180

Query: 223 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 282
             AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 283 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSR 342
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW ++VAAS  DR+ + R
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 343 VQLGNRNIYQGYTINTFDLLGKQYPLIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGK 402
           V LGN     G ++NTF+L G ++P++Y     N+S   + + + +CS   VD  LV+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 403 ILLCDSILAPATFASFSGAVGVIMNDAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNF 462
           I+LCD  L     A  +GA+GVI+ +  + D+A   P P+S LG     ++K+Y+ S   
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 463 PTATIFKSNAVNDTTAPLVVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSS-- 522
           P A I ++  + D  AP V SFSSRGP+    ++LKPD++APG+EILAA+SP+AS SS  
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 523 GVRDSRKTLYNIISGTSMSCPHATAAAVYVKTFHPSWSPAAIKSALMTTATPLNAKLNPQ 582
              D R   Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MTTATP+N K NP+
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 583 AEFAYGAGHINPLKAVHPGLVYDASESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGR 642
            EFAYG+G INP KA  PGLVY+    DY++ LC +G+ +  +   SG N  C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 643 VWDLNYPSFALSSTPSESINQFFRRTLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFN 702
           V DLNYP+     +  +  N  F+RT+TNVG   S Y+A ++     L I++ P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 703 AIGQKKSFTLTVRGSVSQ--SIVSASLVWSDGYHNVRSPITVYVV 744
            + +KKSF +T+ G   +  S VS+S+VWSDG H+VRSPI  Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of Spg010015 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 647.9 bits (1670), Expect = 1.0e-185
Identity = 352/738 (47.70%), Postives = 477/738 (64.63%), Query Frame = 0

Query: 17  LNLLCSLLVAS--SLDSDDDGRKIYIVYLGNKPEDTASTP-SHHMRMLEEVVGSTFAPES 76
           L+ + +LLV S  S   DD  +++YIVY+G  P      P SHH  +L++V G +   + 
Sbjct: 10  LSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQDR 69

Query: 77  LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGFT--KNVPR 136
           L+ +YKRSFNGF  +LTE E + +++ + VVSVFP++  +L TT SW+FMG    K   R
Sbjct: 70  LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 129

Query: 137 VKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGARAYRS 196
              +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+   NF CN K+IGAR Y  
Sbjct: 130 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTP 189

Query: 197 DNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICW 256
                PE   S RD+ GHG+HTAS  AG  V   S YGL  GT RGGVP+ARIAVYK+C 
Sbjct: 190 KLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCD 249

Query: 257 SD--GCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTSNS 316
                C    ILAAFDDAIAD VDII++S+G      +  D++AIGAFH+M  GILT N 
Sbjct: 250 PGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNG 309

Query: 317 AGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYPLI 376
           AGN+GP+  TI + +PW  +VAAS M+R  +++V LGN     G ++N+FDL GK+YPL+
Sbjct: 310 AGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLV 369

Query: 377 YAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMNDA 436
           Y  +A   S     SS+ FCS   +D   V+GKI+LCD+   P    +      ++ N  
Sbjct: 370 YGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNP- 429

Query: 437 GVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSRGP 496
             +D A  +  P S L     N V +Y++S   P A + KS  + +  AP+V S+SSRGP
Sbjct: 430 -YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGP 489

Query: 497 NPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHATAAAVY 556
           NP  +DILKPD+TAPG EILAA+SP    S    D+R   Y +ISGTSMSCPH    A Y
Sbjct: 490 NPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTVISGTSMSCPHVAGVAAY 549

Query: 557 VKTFHPSWSPAAIKSALMTTATPLNAKLNPQ---AEFAYGAGHINPLKAVHPGLVYDASE 616
           +KTFHP WSP+ I+SA+MTTA P+NA  +P    AEFAYGAGH++P+ A+HPGLVY+A++
Sbjct: 550 IKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANK 609

Query: 617 SDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW-DLNYPSFALSSTPSESINQFFRR 676
           SD++ FLCG  YT   +R +SGD+S CT+  +  +  +LNYPS +   + ++     FRR
Sbjct: 610 SDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRR 669

Query: 677 TLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGS--VSQSIVSA 736
           T+TNVG   + Y+AK++G+   L + V P VLS  ++ +KKSFT+TV G+   ++++VSA
Sbjct: 670 TVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSA 729

Query: 737 SLVWSDGYHNVRSPITVY 742
            L+WSDG H VRSPI VY
Sbjct: 730 QLIWSDGVHFVRSPIVVY 735

BLAST of Spg010015 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 647.1 bits (1668), Expect = 1.7e-185
Identity = 351/742 (47.30%), Postives = 488/742 (65.77%), Query Frame = 0

Query: 16  FLNLLCSLLVAS----SLDSDDDG-RKIYIVYLGNKPEDTASTP-SHHMRMLEEVVGSTF 75
           F+ L  SLLV S    S D DD G +++YIVYLG+ P     TP S HM +L+E+ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 76  APESLLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGFTKNV 135
               L+ SYK+SFNGF  +LTE E ++++  E VVSVFP+ K  L TT SW+FMG  + +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 136 --PRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGAR 195
              R + +ES+ ++GV+DSGI+PES SFSD G+GPPP KWKGTC    NF CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186

Query: 196 AYRSDNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVY 255
            Y + +    +  ++ RD  GHGTHTAS  AG  V  ++ YGL  GTARGGVP+ARIAVY
Sbjct: 187 DYTAKS----KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVY 246

Query: 256 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTS 315
           K+C ++GC    +++AFDDAIADGVD+IS+S+       +  D IAIGAFH+M  G+LT 
Sbjct: 247 KVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTV 306

Query: 316 NSAGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYP 375
           N+AGN+GP   T+ + +PW  SVAAS  +R  +++V LG+  I  G ++NT+D+ G  YP
Sbjct: 307 NAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYP 366

Query: 376 LIYAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMN 435
           L+Y  +A       +   +R C    +D  LV+GKI+LCDS       A   GAVG I+ 
Sbjct: 367 LVYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKG-LIEAQKLGAVGSIVK 426

Query: 436 DAGVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSR 495
           +    D A     P S+L      ++ +YM+S   P AT+ KS  +++  APLV SFSSR
Sbjct: 427 NP-EPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSR 486

Query: 496 GPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHATAAA 555
           GP+    DILKPD+TAPGVEILAA+SP +S +    D+R+  Y+++SGTSM+CPH    A
Sbjct: 487 GPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVA 546

Query: 556 VYVKTFHPSWSPAAIKSALMTTATPLNAKLN--PQAEFAYGAGHINPLKAVHPGLVYDAS 615
            YVKTFHP WSP+ I+SA+MTTA P+NA  +     EFAYG+GH++P+ A++PGLVY+ +
Sbjct: 547 AYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELT 606

Query: 616 ESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFR 675
           ++D++ FLCG  YT+  +R +SGDNS CT+  S  +  +LNYP+ +   + ++  N  F+
Sbjct: 607 KADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQ 666

Query: 676 RTLTNVGSKVSIYRAKILGAP-QGLTITVNPPVLSFNAIGQKKSFTLTVRGSV--SQSIV 735
           RT+TNVG + S Y AK++  P   L+I V+P VLS  ++ +K+SF +TV      ++  V
Sbjct: 667 RTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPV 726

Query: 736 SASLVWSDGYHNVRSPITVYVV 744
           SA+L+WSDG HNVRSPI VY +
Sbjct: 727 SANLIWSDGTHNVRSPIIVYAM 740

BLAST of Spg010015 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 639.8 bits (1649), Expect = 2.7e-183
Identity = 349/735 (47.48%), Postives = 468/735 (63.67%), Query Frame = 0

Query: 18  NLLCSLLV--ASSLDSDDDGRKIYIVYLGNKPEDTASTP-SHHMRMLEEVVGSTFAPESL 77
           +LL  LLV   SS+ +  D +++YIVY+G+       TP S HM +L+EV G +     L
Sbjct: 9   SLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRL 68

Query: 78  LHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGFTKNV--PRV 137
           + SYKRSFNGF  +LTE E ++++   GVVSVFPN+K  L TT SWDFMG  + +   R 
Sbjct: 69  VRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRN 128

Query: 138 KQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGARAYRSD 197
             VES+ ++GV+DSGI PES SFSD G+GPPP KWKG C    NF CN K+IGAR Y S+
Sbjct: 129 PTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE 188

Query: 198 NFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKICWS 257
                      RD DGHGTHTAST AG  V  AS +G+  GT RGGVP++R+A YK+C  
Sbjct: 189 ---------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTP 248

Query: 258 DGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTSNSAGN 317
            GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH+M  G+LT NSAGN
Sbjct: 249 TGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGN 308

Query: 318 DGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYPLIYAG 377
            GP   ++   +PW L+VAAST +R  V++V LGN     G ++N +++ GK YPL+Y  
Sbjct: 309 SGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGK 368

Query: 378 NAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMNDAGVK 437
           +A   S      S+  C  + VD++ V+GKIL+C             GAVG+I       
Sbjct: 369 SA--ASSACDAESAGLCELSCVDKSRVKGKILVCGG-PGGLKIVESVGAVGLIYR-TPKP 428

Query: 438 DNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSRGPNPE 497
           D A  +PLP++ L T    ++ +Y+ S + P A + K+ A+ + T+P++ SFSSRGPN  
Sbjct: 429 DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTI 488

Query: 498 TYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHATAAAVYVKT 557
             DILKPD+TAPGVEILAA+SP    S    D+R   Y+++SGTSMSCPH    A YVKT
Sbjct: 489 AVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKT 548

Query: 558 FHPSWSPAAIKSALMTTATPLNAKLN--PQAEFAYGAGHINPLKAVHPGLVYDASESDYV 617
           F+P WSP+ I+SA+MTTA P+NA        EFAYG+GH++P+ A +PGLVY+  +SD++
Sbjct: 549 FNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHI 608

Query: 618 RFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSF-ALSSTPSESINQFFRRTLTN 677
            FLCG  YT+ +++ +SG+   C+ A      +LNYPS  A  S    +    F RTLTN
Sbjct: 609 AFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTN 668

Query: 678 VGSKVSIYRAKIL-GAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGSVSQSIV--SASLV 737
           VG+  S Y +K++ G    L + + P VLSF  + +K+SFT+TV GS   S V  SA+L+
Sbjct: 669 VGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLI 728

Query: 738 WSDGYHNVRSPITVY 742
           WSDG HNVRSPI VY
Sbjct: 729 WSDGTHNVRSPIVVY 728

BLAST of Spg010015 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 638.3 bits (1645), Expect = 7.9e-183
Identity = 356/738 (48.24%), Postives = 478/738 (64.77%), Query Frame = 0

Query: 17  LNLLCSLLVAS--SLDSDDDGRKIYIVYLGNKPEDTASTP-SHHMRMLEEVVGSTFAPES 76
           L+ + +LLV S  S D DD  ++ YIVY+G  P      P SHH  +L++V G +   + 
Sbjct: 11  LSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSIEDR 70

Query: 77  LLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNEKKHLHTTRSWDFMGF--TKNVPR 136
           L+ +YKRSFNGF  +LT+ E + +++ + VVSVFPN+K  L TT SW+FMG   +K   R
Sbjct: 71  LVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKR 130

Query: 137 VKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGTCQASANFHCNRKIIGARAYRS 196
              +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+   NF  N K+IGAR Y  
Sbjct: 131 NTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTP 190

Query: 197 DNFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVYKIC- 256
                PE   S RD  GHG+HTAST AG  V   S YGL  GTARGGVP+ARIAVYK+C 
Sbjct: 191 KLEGFPE---SARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCD 250

Query: 257 -WSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGILTSNS 316
              DGC    ILAAFDDAIAD VDII++S+GG     +  D IAIGAFH+M  GIL  NS
Sbjct: 251 PGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNS 310

Query: 317 AGNDGPDYFTIRNFSPWSLSVAASTMDRKLVSRVQLGNRNIYQGYTINTFDLLGKQYPLI 376
           AGN GP+  T+ + +PW  +VAAS  +R  V++V LGN     G ++N+FDL GK+YPL+
Sbjct: 311 AGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLV 370

Query: 377 YAGNAPNISGGFTGSSSRFCSKNSVDRNLVRGKILLCDSILAPATFASFSGAVGVIMNDA 436
           Y  +A +  G    +S+ FCS   +D   V+GKI+LCDS   P   A   GA+  I+   
Sbjct: 371 YGKSASSSCG---AASAGFCSPGCLDSKRVKGKIVLCDSPQNPDE-AQAMGAIASIVRSH 430

Query: 437 GVKDNARSYPLPSSYLGTTAGNNVKTYMSSNNFPTATIFKSNAVNDTTAPLVVSFSSRGP 496
              D A  +  P S L     N V +YM+S   P A + KS  + +  AP+V S+ SRGP
Sbjct: 431 RT-DVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGP 490

Query: 497 NPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRKTLYNIISGTSMSCPHATAAAVY 556
           N    DILKPD+TAPG EI+AA+SP A  S  + D+R+  Y++ +GTSMSCPH    A Y
Sbjct: 491 NTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAY 550

Query: 557 VKTFHPSWSPAAIKSALMTTATPLNAKLNP---QAEFAYGAGHINPLKAVHPGLVYDASE 616
           +K+FHP WSP+ I+SA+MTTA P+NA  +P    AEFAYGAGH++P+ A+HPGLVY+A++
Sbjct: 551 LKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANK 610

Query: 617 SDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV-WDLNYPSFALSSTPSESINQFFRR 676
           SD++ FLCG  YT   +R +SGD+S CT+  +  +  +LNYPS     + ++     FRR
Sbjct: 611 SDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRR 670

Query: 677 TLTNVGSKVSIYRAKILGAPQGLTITVNPPVLSFNAIGQKKSFTLTVRGS--VSQSIVSA 736
           T+TNVG   + Y+AK++G+   L + V P VLS  ++ +KKSFT+T  G+   ++++VSA
Sbjct: 671 TVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSA 730

Query: 737 SLVWSDGYHNVRSPITVY 742
            L+WSDG H VRSPI VY
Sbjct: 731 QLIWSDGVHFVRSPIVVY 735