Spg009994 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg009994 vs. NCBI nr
Match: XP_022150493.1 (protein PIN-LIKES 2-like [Momordica charantia])

HSP 1 Score: 717.2 bits (1850), Expect = 9.0e-203
Identity = 376/467 (80.51%), Postives = 409/467 (87.58%), Query Frame = 0

Query: 1   MSEYFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLV 60
           MS YFRLLYE  ++S+EED LAAIVPL+KLLCLTVIGLVLAHPKTK+IP+ TF+LL KLV
Sbjct: 1   MSGYFRLLYERSVKSSEEDALAAIVPLLKLLCLTVIGLVLAHPKTKVIPRATFKLLSKLV 60

Query: 61  FVLFLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMT 120
           F LFLPCLIFTELG+SITLE+ +QWWFIP+NVLLST IGC LGYLVV  CHPPPQLNR+T
Sbjct: 61  FALFLPCLIFTELGRSITLENLSQWWFIPINVLLSTGIGCVLGYLVVIICHPPPQLNRLT 120

Query: 121 VIVTAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMME 180
           VI+TAFGNTGNLPLA+VGSVCHT +NPFG +CHSSGVAYVSLAQW+SVILAYTFVYHMME
Sbjct: 121 VIMTAFGNTGNLPLAIVGSVCHTGDNPFGQHCHSSGVAYVSLAQWVSVILAYTFVYHMME 180

Query: 181 PSPECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQ 240
           P  +C EI DE I++EEE+AD D  RPLLVEAEWPG+EDKETEHSKT  IARIF+S S  
Sbjct: 181 PPLDCCEIVDERIEMEEEQADNDFSRPLLVEAEWPGIEDKETEHSKTPTIARIFNSISST 240

Query: 241 SQSTSTDIKLSVGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQ 300
           SQST                STDIELSGG STDSPK   CLA+P VVR+M+IVAEQTPIQ
Sbjct: 241 SQST----------------STDIELSGGCSTDSPKSINCLAQPDVVRRMKIVAEQTPIQ 300

Query: 301 HILQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLA 360
           HI QPPTIASLVTIIIGLIP+LKSLFFDYDAPLSFV DSLEILARATVPSVML+LGGMLA
Sbjct: 301 HIFQPPTIASLVTIIIGLIPRLKSLFFDYDAPLSFVKDSLEILARATVPSVMLVLGGMLA 360

Query: 361 EYPNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPS 420
           E PN S L H+TTI IIIARLLVLPL GIGIVTLADKLNFLVSGDPM+RFVLLLQYTTPS
Sbjct: 361 EGPNISKLSHQTTISIIIARLLVLPLFGIGIVTLADKLNFLVSGDPMFRFVLLLQYTTPS 420

Query: 421 AILLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
           AILLGAIASLRGYAVRE SALLFWQHIFALFSLS+Y++IYFKIV YV
Sbjct: 421 AILLGAIASLRGYAVREASALLFWQHIFALFSLSVYIIIYFKIVVYV 451

BLAST of Spg009994 vs. NCBI nr
Match: XP_023541300.1 (protein PIN-LIKES 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 713.0 bits (1839), Expect = 1.7e-201
Identity = 372/465 (80.00%), Postives = 411/465 (88.39%), Query Frame = 0

Query: 4   YFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVL 63
           YFRLLYE  I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF L
Sbjct: 3   YFRLLYETGIEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFAL 62

Query: 64  FLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIV 123
           FLPCLIFTELGQSITLES  QWWFIPVNVLLSTAIGC LGYLVVA CHPPP+ NR+TV++
Sbjct: 63  FLPCLIFTELGQSITLESVTQWWFIPVNVLLSTAIGCVLGYLVVALCHPPPEFNRLTVVM 122

Query: 124 TAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSP 183
           TAFGNTGNL LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P P
Sbjct: 123 TAFGNTGNLTLAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPP 182

Query: 184 ECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQS 243
           EC+ + DEGI+IEEERAD D+ RPLL EAEWP +EDKETEHS T+ I RI +S SI  Q+
Sbjct: 183 ECNVVLDEGIEIEEERADNDLSRPLLAEAEWPSIEDKETEHSMTSFIPRILNSISITPQT 242

Query: 244 TSTDIKLS-VGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHI 303
           TSTDI++S  GD  D+P+  DIEL+G   T+SPK T+ LA+PRVVR++  +AEQTPIQHI
Sbjct: 243 TSTDIEISGGGDCTDNPKPNDIELAGDNRTNSPKSTEFLAKPRVVRRLSHLAEQTPIQHI 302

Query: 304 LQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEY 363
           LQPPTIA L  IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE 
Sbjct: 303 LQPPTIAFLAAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAEC 362

Query: 364 PNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAI 423
            NTS LGHRTTIGIIIARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAI
Sbjct: 363 SNTSTLGHRTTIGIIIARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAI 422

Query: 424 LLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
           LLGA+ASL GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 423 LLGALASLGGYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 467

BLAST of Spg009994 vs. NCBI nr
Match: XP_022945293.1 (protein PIN-LIKES 2-like isoform X1 [Cucurbita moschata] >XP_022945294.1 protein PIN-LIKES 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 711.1 bits (1834), Expect = 6.4e-201
Identity = 373/465 (80.22%), Postives = 409/465 (87.96%), Query Frame = 0

Query: 4   YFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVL 63
           YFRLLYE  I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF L
Sbjct: 3   YFRLLYETGIEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFAL 62

Query: 64  FLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIV 123
           FLPCLIFTELGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+TV++
Sbjct: 63  FLPCLIFTELGQSITLESVTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTVVM 122

Query: 124 TAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSP 183
           TAFGNTGNL LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P P
Sbjct: 123 TAFGNTGNLALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPP 182

Query: 184 ECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQS 243
           EC+ I DEGI+I EERAD D+ RPLL EAEWP +EDKETEHS T+ I RI +S S   Q+
Sbjct: 183 ECNVILDEGIEIVEERADNDLSRPLLAEAEWPSIEDKETEHSMTSFIPRILNSISTTPQT 242

Query: 244 TSTDIKLS-VGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHI 303
           TSTDI++S  GD  D+P+  DIEL+G   T+SPK T+ LAEPRVVR++  VAEQTPIQHI
Sbjct: 243 TSTDIEISGGGDCTDNPKPNDIELAGDNRTNSPKSTEFLAEPRVVRRLSHVAEQTPIQHI 302

Query: 304 LQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEY 363
           LQPPTIA L  IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE 
Sbjct: 303 LQPPTIAFLAAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAEC 362

Query: 364 PNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAI 423
            NTS LGHRTTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAI
Sbjct: 363 SNTSTLGHRTTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAI 422

Query: 424 LLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
           LLGA+ASL GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 423 LLGALASLGGYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 467

BLAST of Spg009994 vs. NCBI nr
Match: KAG6573917.1 (Protein PIN-LIKES 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 709.5 bits (1830), Expect = 1.9e-200
Identity = 371/465 (79.78%), Postives = 409/465 (87.96%), Query Frame = 0

Query: 4   YFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVL 63
           YFRLLYE  I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF L
Sbjct: 3   YFRLLYETGIEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFAL 62

Query: 64  FLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIV 123
           FLPCLIFTELGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+TV++
Sbjct: 63  FLPCLIFTELGQSITLESVTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTVVM 122

Query: 124 TAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSP 183
           TAFGNTGNL LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P P
Sbjct: 123 TAFGNTGNLALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPP 182

Query: 184 ECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQS 243
           EC+ + DEGI+I EERAD D+ RPLL EAEWP +EDKETEHS T+ I RI +S S   Q+
Sbjct: 183 ECNVVLDEGIEIVEERADNDLSRPLLAEAEWPSIEDKETEHSMTSFIPRILNSISTTPQT 242

Query: 244 TSTDIKLS-VGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHI 303
           TSTDI++S  GD  D+P+  DIEL+G   T+SPK T+ LAEPRVVR++  +AEQTPIQHI
Sbjct: 243 TSTDIEISGGGDCTDNPKPNDIELAGDNRTNSPKSTEFLAEPRVVRRLSHLAEQTPIQHI 302

Query: 304 LQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEY 363
           LQPPTIA L  IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE 
Sbjct: 303 LQPPTIAFLAAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAEC 362

Query: 364 PNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAI 423
            NTS LGHRTTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAI
Sbjct: 363 SNTSTLGHRTTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAI 422

Query: 424 LLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
           LLGA+ASL GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 423 LLGALASLGGYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 467

BLAST of Spg009994 vs. NCBI nr
Match: XP_022968440.1 (protein PIN-LIKES 2-like isoform X1 [Cucurbita maxima] >XP_022968441.1 protein PIN-LIKES 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 706.1 bits (1821), Expect = 2.1e-199
Identity = 369/465 (79.35%), Postives = 409/465 (87.96%), Query Frame = 0

Query: 4   YFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVL 63
           YFRLLYE  I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF L
Sbjct: 8   YFRLLYETGIEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFAL 67

Query: 64  FLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIV 123
           FLPCLIFTELGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+T+++
Sbjct: 68  FLPCLIFTELGQSITLESLTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTLVM 127

Query: 124 TAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSP 183
           TAFGNTGNL LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P P
Sbjct: 128 TAFGNTGNLALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPP 187

Query: 184 ECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQS 243
           EC+ + DEGI+IEEERAD D+GRPLL EAEWP +E KETEHS T+ I RI +S SI  Q+
Sbjct: 188 ECNVVLDEGIEIEEERADNDLGRPLLAEAEWPSIEGKETEHSVTSFIPRILNSISITPQT 247

Query: 244 TSTDIKLS-VGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHI 303
           TSTDI+ S  GD  D+P+  DIEL+G   T+SPK T+ LA PRV+R++  +AEQTPIQHI
Sbjct: 248 TSTDIESSGGGDCTDNPKPNDIELAGDNRTNSPKSTEFLAGPRVLRRLSHLAEQTPIQHI 307

Query: 304 LQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEY 363
           LQPPTI+ L  IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE 
Sbjct: 308 LQPPTISFLAAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAEC 367

Query: 364 PNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAI 423
            NTS LGHRTTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAI
Sbjct: 368 SNTSTLGHRTTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAI 427

Query: 424 LLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
           LLGA+ASL GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 428 LLGALASLGGYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 472

BLAST of Spg009994 vs. ExPASy Swiss-Prot
Match: Q9C999 (Protein PIN-LIKES 2 OS=Arabidopsis thaliana OX=3702 GN=PILS2 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 6.5e-164
Identity = 302/454 (66.52%), Postives = 366/454 (80.62%), Query Frame = 0

Query: 12  DIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFT 71
           ++ S   DIL+ +VPL+KL+CLTVIGL+LAHPKT+++P+ TFRLL KLVF LFLPCLIFT
Sbjct: 8   NVNSRVVDILSGVVPLLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLVFALFLPCLIFT 67

Query: 72  ELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGN 131
           ELG+SITL++  QWWFIPVNVLLS  +G  +GYLVV  C PPP+ NR T+++TAFGNTGN
Sbjct: 68  ELGESITLDNIVQWWFIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFTIVMTAFGNTGN 127

Query: 132 LPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSE-IED 191
           L LA+V SVCHT  NPFGPNC+S GV+YVS AQW++VIL YT VYHMMEP  E  E +E+
Sbjct: 128 LLLAIVSSVCHTKTNPFGPNCNSRGVSYVSFAQWVAVILVYTVVYHMMEPPLEYYEVVEE 187

Query: 192 EGIKIEEERADG-DIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIK 251
           EG++IEE   +  D  RPLLVEAEWPG+EDKETEH KT  IAR+F+S S  SQ++  ++ 
Sbjct: 188 EGVEIEEINVENHDASRPLLVEAEWPGIEDKETEHCKTPFIARVFNSISSFSQTSFPEVD 247

Query: 252 LSVGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIA 311
           L  G+             GGES+ SP+  +CLAEPRV+R++R+VAEQTP++HILQPPTIA
Sbjct: 248 LG-GE------------YGGESS-SPRSIQCLAEPRVMRRIRVVAEQTPVKHILQPPTIA 307

Query: 312 SLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILG 371
           SL+ IIIG +PQLKS+ F YDAPLSF+TDSL I+  A VPSVML+LGGML+E PN S LG
Sbjct: 308 SLLAIIIGSVPQLKSVVFGYDAPLSFITDSLNIMGSAMVPSVMLVLGGMLSEGPNESTLG 367

Query: 372 HRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIAS 431
            RTTIGI +ARLLVLPL+GIGIV  ADKL  + S DPM++FVLLLQY+TPSAILLGAIAS
Sbjct: 368 LRTTIGISVARLLVLPLVGIGIVMSADKLGLISSADPMFKFVLLLQYSTPSAILLGAIAS 427

Query: 432 LRGYAVREVSALLFWQHIFALFSLSLYVVIYFKI 464
           LRGYAVRE SALLFWQHIFAL SL+ Y+VI+FK+
Sbjct: 428 LRGYAVREASALLFWQHIFALLSLTFYIVIFFKL 447

BLAST of Spg009994 vs. ExPASy Swiss-Prot
Match: Q9LZN2 (Protein PIN-LIKES 6 OS=Arabidopsis thaliana OX=3702 GN=PILS6 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.4e-73
Identity = 168/447 (37.58%), Postives = 254/447 (56.82%), Query Frame = 0

Query: 20  ILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITL 79
           I  A++P+ K+  +  +GL++A     I+P    +LL  LVF L LPCLIF++LGQ++TL
Sbjct: 28  IKIAVMPIAKVFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCLIFSQLGQAVTL 87

Query: 80  ESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGS 139
           +   QWWFIPVNV+L T  G  +G++V +   PP    + T+I    GN GN+PL ++ +
Sbjct: 88  QKMLQWWFIPVNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGNIGNVPLVLLAA 147

Query: 140 VCHTSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEIEDEGIKIEE 199
           +C  ++NPFG +  C   G AY+S  QW+  I+ YT+VY M  P PE  + E+E + ++ 
Sbjct: 148 LCRDTSNPFGDSEKCSIDGTAYISFGQWVGAIILYTYVYQMFAPPPEGFDAEEENLALKT 207

Query: 200 ERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIKLSVGDNAD 259
              D    +  L+   +P                + FS T       ST+          
Sbjct: 208 LPVDAAPEQVPLLTQNFP----------------KDFSPTQDLLPVQSTE---------- 267

Query: 260 SPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIG 319
            P    +   G                ++ +    + E+  ++ I+QP  +AS++ +I+G
Sbjct: 268 -PRGRGVSRKG----------------KIAQIFVFLYEKLKLKQIVQPAIVASILAMILG 327

Query: 320 LIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILGHRTTIGII 379
            IP  K L F   APL F TDS  IL  A +P ++L LGG L   P +S LG +TT  II
Sbjct: 328 AIPFTKKLIFTNGAPLFFFTDSCMILGDAMIPCILLALGGNLINGPGSSKLGFKTTAAII 387

Query: 380 IARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVRE 439
           I RL+++P +G+GIVT+ADKL FL + D M+RFVLLLQ+T P+++L GA+A+LRG   RE
Sbjct: 388 IGRLVLVPPVGLGIVTVADKLGFLPADDKMFRFVLLLQHTMPTSVLSGAVANLRGCG-RE 430

Query: 440 VSALLFWQHIFALFSLSLYVVIYFKIV 465
            +A+LFW HIFA+FS++ ++V+Y  I+
Sbjct: 448 SAAVLFWVHIFAIFSMAGWMVLYINIL 430

BLAST of Spg009994 vs. ExPASy Swiss-Prot
Match: Q9SHL8 (Protein PIN-LIKES 5 OS=Arabidopsis thaliana OX=3702 GN=PILS5 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.5e-46
Identity = 126/444 (28.38%), Postives = 222/444 (50.00%), Query Frame = 0

Query: 23  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 82
           A +P++++L ++++G  +A  + K+ P E    + K+VFVLF P L+F  L Q++TLE  
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 142
             WWF+PVN+ L+  IG  LG+LVV    PPP L  + V   + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEIEDEGIKIEEERA 202
              +PFG    C + G++Y S +  +     +T+ + +++ S    +  +E  KI  + +
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 203 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIKLSVGDNADSPE 262
           + D+                E +H KT L+                           +PE
Sbjct: 190 NSDL----------------EADH-KTHLLG--------------------------APE 249

Query: 263 STDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 322
                          K  K + E     +  +      ++ +L PPT+ +++  I G + 
Sbjct: 250 D--------------KENKVVKEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVR 309

Query: 323 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILGHRTTIGIIIAR 382
            L++L    DAPL  V  + ++L   T+P + +ILGG L +   +S +     +GI+  R
Sbjct: 310 WLRNLIIGDDAPLRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRSSAVKPMVVLGIVCVR 369

Query: 383 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 442
            + +P++GIGIV  A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S 
Sbjct: 370 YIAMPIIGIGIVLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSV 395

Query: 443 LLFWQHIFALFSLSLYVVIYFKIV 465
           L+ W ++ A+ +L+++  I+  ++
Sbjct: 430 LMLWTYLVAILALTVWSTIFLHLL 395

BLAST of Spg009994 vs. ExPASy Swiss-Prot
Match: Q9FKY4 (Protein PIN-LIKES 7 OS=Arabidopsis thaliana OX=3702 GN=PILS7 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 122/445 (27.42%), Postives = 223/445 (50.11%), Query Frame = 0

Query: 23  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 82
           A +P++++L ++V+G  LA     ++  +T R + KLVFV+F PC++F  L +++TL+  
Sbjct: 10  ASMPIVQVLLISVLGAFLATDYCSLLSADTRRSVNKLVFVVFTPCIMFANLAETVTLQDI 69

Query: 83  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 142
             WWF+P+NV ++  +G  LG+LVV   +P PQL+ + +   A GN GNL L +V ++C 
Sbjct: 70  ISWWFMPINVGITFLVGGILGWLVVKLLNPKPQLHGLIIATCASGNMGNLMLILVPAICD 129

Query: 143 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEIEDEGIKIEEERA 202
              +PFG    C S G++Y S +  +     +T+ Y ++  S           +     A
Sbjct: 130 EEGSPFGNRSVCRSIGLSYASFSMALGGFYIWTYSYQLVRSS---------ATQFRALEA 189

Query: 203 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIKLSVGDNADSPE 262
            G +  P           +K+ +    AL+ +                           +
Sbjct: 190 AGLVKSP-----------NKDIDSDPHALLLK-------------------------PHQ 249

Query: 263 STDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 322
           + D+E+ G +   +  + K L                 ++ +  PPTI +++  + G   
Sbjct: 250 NQDLEIQGKQKVSTRTYIKDLL-------------HQILEELFAPPTIGAILGFVFGATN 309

Query: 323 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILGHRTTIGIIIAR 382
            L++L    +APL  + DS+++L   T+P + LILGG L +   +S +     +G+II R
Sbjct: 310 WLRNLIIGENAPLRVIQDSVKLLGEGTIPCITLILGGNLIQGLRSSAVKKSVIVGVIIVR 369

Query: 383 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 442
            ++LP++G+G+V LA  L +L   DP++R+VL+LQ+  P A+ +  +A L   A  E S 
Sbjct: 370 YILLPVVGVGVVQLAGNLGYL-PPDPLFRYVLMLQFALPPAMNISTMAQLFDVAQDECSV 395

Query: 443 LLFWQHIFALFSLSLYVVIYFKIVA 466
           +  W ++ A  +L+++  I+  I++
Sbjct: 430 IFLWTYLVASLALTVWSTIFLSILS 395

BLAST of Spg009994 vs. ExPASy Swiss-Prot
Match: F4HWB6 (Protein PIN-LIKES 1 OS=Arabidopsis thaliana OX=3702 GN=PILS1 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.8e-44
Identity = 139/462 (30.09%), Postives = 225/462 (48.70%), Query Frame = 0

Query: 6   RLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFL 65
           R  Y M +R  +  I ++I P+ K+L +T IG  LA  +  I+  +  + L  +VF +F 
Sbjct: 82  RSFYSMRMRLLDLFITSSI-PVAKILLITGIGFYLALDQVNILNHDARKQLNNIVFYVFS 141

Query: 66  PCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTA 125
           P L+ + L ++IT ES  + WF+P+NVLL+  IG  LG++V+    PP  L  + V   A
Sbjct: 142 PSLVASSLSETITYESMVKMWFMPLNVLLTFIIGSFLGWIVIKITKPPSHLRGIIVGCCA 201

Query: 126 FGNTGNLPLAVVGSVCHTSNNPFG--PNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSP 185
            GN GN+PL ++ ++C+   +PFG   +C   G+ Y++L+  I  I  +T+VY++M    
Sbjct: 202 AGNLGNMPLIIIPAICNEKGSPFGDPESCEKFGLGYIALSMAIGAIYIWTYVYNLMR--- 261

Query: 186 ECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQS 245
                                                        ++A     T+I S S
Sbjct: 262 ---------------------------------------------MLANPAGETAINSTS 321

Query: 246 TSTDIKLSVGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHIL 305
           ++  +         SP+    E  G                +V +++  VAE+  ++ I 
Sbjct: 322 STMPL--------ISPKVEVAEQVG-------------TWGKVKQRVCSVAEKINLRTIF 381

Query: 306 QPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYP 365
            P TIA+L+ + +GL P L+ L     APL  + DS+ +L    +P + LI+GG L    
Sbjct: 382 APSTIAALIALAVGLNPLLRKLLVGNTAPLRVIEDSVSLLGDGAIPVLTLIVGGNLLNGL 441

Query: 366 NTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAIL 425
             S +     +G+++ R L+LP+LG+ IV  A  L  LV+ +P+Y+FVLLLQY  P A+ 
Sbjct: 442 RGSGINKSVIMGVVVVRYLLLPILGVFIVRGAHYLG-LVTSEPLYQFVLLLQYVVPPAMN 472

Query: 426 LGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVA 466
           LG I  L G    E S +LFW +  A  SL+++   +  +VA
Sbjct: 502 LGTITQLFGSGESECSVILFWSYALASVSLTVWPTFFMWLVA 472

BLAST of Spg009994 vs. ExPASy TrEMBL
Match: A0A6J1D9L3 (protein PIN-LIKES 2-like OS=Momordica charantia OX=3673 GN=LOC111018630 PE=4 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 4.4e-203
Identity = 376/467 (80.51%), Postives = 409/467 (87.58%), Query Frame = 0

Query: 1   MSEYFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLV 60
           MS YFRLLYE  ++S+EED LAAIVPL+KLLCLTVIGLVLAHPKTK+IP+ TF+LL KLV
Sbjct: 1   MSGYFRLLYERSVKSSEEDALAAIVPLLKLLCLTVIGLVLAHPKTKVIPRATFKLLSKLV 60

Query: 61  FVLFLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMT 120
           F LFLPCLIFTELG+SITLE+ +QWWFIP+NVLLST IGC LGYLVV  CHPPPQLNR+T
Sbjct: 61  FALFLPCLIFTELGRSITLENLSQWWFIPINVLLSTGIGCVLGYLVVIICHPPPQLNRLT 120

Query: 121 VIVTAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMME 180
           VI+TAFGNTGNLPLA+VGSVCHT +NPFG +CHSSGVAYVSLAQW+SVILAYTFVYHMME
Sbjct: 121 VIMTAFGNTGNLPLAIVGSVCHTGDNPFGQHCHSSGVAYVSLAQWVSVILAYTFVYHMME 180

Query: 181 PSPECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQ 240
           P  +C EI DE I++EEE+AD D  RPLLVEAEWPG+EDKETEHSKT  IARIF+S S  
Sbjct: 181 PPLDCCEIVDERIEMEEEQADNDFSRPLLVEAEWPGIEDKETEHSKTPTIARIFNSISST 240

Query: 241 SQSTSTDIKLSVGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQ 300
           SQST                STDIELSGG STDSPK   CLA+P VVR+M+IVAEQTPIQ
Sbjct: 241 SQST----------------STDIELSGGCSTDSPKSINCLAQPDVVRRMKIVAEQTPIQ 300

Query: 301 HILQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLA 360
           HI QPPTIASLVTIIIGLIP+LKSLFFDYDAPLSFV DSLEILARATVPSVML+LGGMLA
Sbjct: 301 HIFQPPTIASLVTIIIGLIPRLKSLFFDYDAPLSFVKDSLEILARATVPSVMLVLGGMLA 360

Query: 361 EYPNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPS 420
           E PN S L H+TTI IIIARLLVLPL GIGIVTLADKLNFLVSGDPM+RFVLLLQYTTPS
Sbjct: 361 EGPNISKLSHQTTISIIIARLLVLPLFGIGIVTLADKLNFLVSGDPMFRFVLLLQYTTPS 420

Query: 421 AILLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
           AILLGAIASLRGYAVRE SALLFWQHIFALFSLS+Y++IYFKIV YV
Sbjct: 421 AILLGAIASLRGYAVREASALLFWQHIFALFSLSVYIIIYFKIVVYV 451

BLAST of Spg009994 vs. ExPASy TrEMBL
Match: A0A6J1G0E7 (protein PIN-LIKES 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449580 PE=4 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 3.1e-201
Identity = 373/465 (80.22%), Postives = 409/465 (87.96%), Query Frame = 0

Query: 4   YFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVL 63
           YFRLLYE  I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF L
Sbjct: 3   YFRLLYETGIEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFAL 62

Query: 64  FLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIV 123
           FLPCLIFTELGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+TV++
Sbjct: 63  FLPCLIFTELGQSITLESVTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTVVM 122

Query: 124 TAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSP 183
           TAFGNTGNL LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P P
Sbjct: 123 TAFGNTGNLALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPP 182

Query: 184 ECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQS 243
           EC+ I DEGI+I EERAD D+ RPLL EAEWP +EDKETEHS T+ I RI +S S   Q+
Sbjct: 183 ECNVILDEGIEIVEERADNDLSRPLLAEAEWPSIEDKETEHSMTSFIPRILNSISTTPQT 242

Query: 244 TSTDIKLS-VGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHI 303
           TSTDI++S  GD  D+P+  DIEL+G   T+SPK T+ LAEPRVVR++  VAEQTPIQHI
Sbjct: 243 TSTDIEISGGGDCTDNPKPNDIELAGDNRTNSPKSTEFLAEPRVVRRLSHVAEQTPIQHI 302

Query: 304 LQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEY 363
           LQPPTIA L  IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE 
Sbjct: 303 LQPPTIAFLAAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAEC 362

Query: 364 PNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAI 423
            NTS LGHRTTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAI
Sbjct: 363 SNTSTLGHRTTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAI 422

Query: 424 LLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
           LLGA+ASL GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 423 LLGALASLGGYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 467

BLAST of Spg009994 vs. ExPASy TrEMBL
Match: A0A6J1HUV6 (protein PIN-LIKES 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467687 PE=4 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 1.0e-199
Identity = 369/465 (79.35%), Postives = 409/465 (87.96%), Query Frame = 0

Query: 4   YFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVL 63
           YFRLLYE  I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF L
Sbjct: 8   YFRLLYETGIEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFAL 67

Query: 64  FLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIV 123
           FLPCLIFTELGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+T+++
Sbjct: 68  FLPCLIFTELGQSITLESLTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTLVM 127

Query: 124 TAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSP 183
           TAFGNTGNL LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P P
Sbjct: 128 TAFGNTGNLALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPP 187

Query: 184 ECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQS 243
           EC+ + DEGI+IEEERAD D+GRPLL EAEWP +E KETEHS T+ I RI +S SI  Q+
Sbjct: 188 ECNVVLDEGIEIEEERADNDLGRPLLAEAEWPSIEGKETEHSVTSFIPRILNSISITPQT 247

Query: 244 TSTDIKLS-VGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHI 303
           TSTDI+ S  GD  D+P+  DIEL+G   T+SPK T+ LA PRV+R++  +AEQTPIQHI
Sbjct: 248 TSTDIESSGGGDCTDNPKPNDIELAGDNRTNSPKSTEFLAGPRVLRRLSHLAEQTPIQHI 307

Query: 304 LQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEY 363
           LQPPTI+ L  IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE 
Sbjct: 308 LQPPTISFLAAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAEC 367

Query: 364 PNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAI 423
            NTS LGHRTTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAI
Sbjct: 368 SNTSTLGHRTTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAI 427

Query: 424 LLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
           LLGA+ASL GYA+REVSALLFWQHIFALFSLS+YVVIYFK+V+YV
Sbjct: 428 LLGALASLGGYAIREVSALLFWQHIFALFSLSVYVVIYFKLVSYV 472

BLAST of Spg009994 vs. ExPASy TrEMBL
Match: A0A6J1HTI4 (protein PIN-LIKES 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467687 PE=4 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 5.3e-185
Identity = 346/453 (76.38%), Postives = 387/453 (85.43%), Query Frame = 0

Query: 4   YFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVL 63
           YFRLLYE  I  +EED+LAAIVPLMKLLCL VIGL+LAHPKTKIIPKETF LLCKLVF L
Sbjct: 8   YFRLLYETGIEFSEEDLLAAIVPLMKLLCLAVIGLILAHPKTKIIPKETFSLLCKLVFAL 67

Query: 64  FLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIV 123
           FLPCLIFTELGQSITLES  QWWFIPVNVLLSTAIGCALGYLVVA CHPPP+ NR+T+++
Sbjct: 68  FLPCLIFTELGQSITLESLTQWWFIPVNVLLSTAIGCALGYLVVALCHPPPEFNRLTLVM 127

Query: 124 TAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSP 183
           TAFGNTGNL LAVVGSVCHT++NPFGPNCHSSGV YVSLAQW+SVILAYT VYHMM+P P
Sbjct: 128 TAFGNTGNLALAVVGSVCHTADNPFGPNCHSSGVTYVSLAQWVSVILAYTLVYHMMKPPP 187

Query: 184 ECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQS 243
           EC+ + DEGI+IEEERAD D+GRPLL EAEWP +E KETEHS T+ I RI +S SI  Q+
Sbjct: 188 ECNVVLDEGIEIEEERADNDLGRPLLAEAEWPSIEGKETEHSVTSFIPRILNSISITPQT 247

Query: 244 TSTDIKLS-VGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHI 303
           TSTDI+ S  GD  D+P+  DIEL+G   T+SPK T+ LA PRV+R++  +AEQTPIQHI
Sbjct: 248 TSTDIESSGGGDCTDNPKPNDIELAGDNRTNSPKSTEFLAGPRVLRRLSHLAEQTPIQHI 307

Query: 304 LQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEY 363
           LQPPTI+ L  IIIGLIP LKSLFFDY+APLS V DSLEILARATVPSVMLILGG+LAE 
Sbjct: 308 LQPPTISFLAAIIIGLIPPLKSLFFDYNAPLSVVKDSLEILARATVPSVMLILGGLLAEC 367

Query: 364 PNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAI 423
            NTS LGHRTTIGII ARLLVLP+LGIG+V LADKLNFLVSGD MYRFVLLLQYTTPSAI
Sbjct: 368 SNTSTLGHRTTIGIITARLLVLPVLGIGMVLLADKLNFLVSGDQMYRFVLLLQYTTPSAI 427

Query: 424 LLGAIASLRGYAVREVSALLFWQHIFALFSLSL 456
           LLGA+ASL GYA+R + ALL    + +L  + L
Sbjct: 428 LLGALASLGGYAIR-IHALLLMMSVESLMDIFL 459

BLAST of Spg009994 vs. ExPASy TrEMBL
Match: A0A6A6MXI7 (ABC transporter domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_041171 PE=4 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 4.4e-179
Identity = 334/467 (71.52%), Postives = 385/467 (82.44%), Query Frame = 0

Query: 1    MSEYFRLLYEMDIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLV 60
            MS Y    YE ++RS+ E+++ AIVPLMKLLCL +IGL+LAHPKT+IIP+ TF+LL KLV
Sbjct: 682  MSGYITAFYENNMRSSGENLVTAIVPLMKLLCLIIIGLILAHPKTQIIPRATFKLLSKLV 741

Query: 61   FVLFLPCLIFTELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMT 120
            F LFLPCLIFTELG+SITLE+FA+WWFIPVNVL+ST IG  LG LVVA CHPPPQ NR T
Sbjct: 742  FALFLPCLIFTELGESITLENFARWWFIPVNVLVSTIIGFFLGLLVVAICHPPPQYNRFT 801

Query: 121  VIVTAFGNTGNLPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMME 180
            +I+TAFGNTGNLPLA+VGSVCHT ++PFG +CHSSGVAYVS AQW++VIL YT VYHMME
Sbjct: 802  IIMTAFGNTGNLPLAIVGSVCHTKDSPFGAHCHSSGVAYVSFAQWVAVILVYTLVYHMME 861

Query: 181  PSPECSEIEDEGIKIEEERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQ 240
            P  +  EI +E  +IEE+R   D+ RPLL+EAEWPG+EDKETEHSKT  IARIF+S S  
Sbjct: 862  PPLQYYEIVEEEFEIEEQRPTPDVSRPLLIEAEWPGIEDKETEHSKTPFIARIFNSISSL 921

Query: 241  SQSTSTDIKLSVGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQ 300
            SQ                    DIEL+G  + +SP+  +CLAEPRVVR++RIVAEQTPI+
Sbjct: 922  SQI----------------NFPDIELAGESTANSPRSIRCLAEPRVVRRIRIVAEQTPIR 981

Query: 301  HILQPPTIASLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLA 360
            HILQPPTIASL  IIIG++PQLK+  F YDAPLSF+TDSLEILA A VPSVMLILGGMLA
Sbjct: 982  HILQPPTIASLFAIIIGMVPQLKAFVFGYDAPLSFITDSLEILAGAMVPSVMLILGGMLA 1041

Query: 361  EYPNTSILGHRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPS 420
            E PN S LG  TTIGI +ARLLVLPLLGIGIV LADKLNFLV GD MYRFVLLLQYTTPS
Sbjct: 1042 EGPNESTLGLPTTIGISVARLLVLPLLGIGIVALADKLNFLVKGDAMYRFVLLLQYTTPS 1101

Query: 421  AILLGAIASLRGYAVREVSALLFWQHIFALFSLSLYVVIYFKIVAYV 468
            AILLGAIASLRGYAV+E SALLFWQH+FALFSLSLY+VIYFK+++Y+
Sbjct: 1102 AILLGAIASLRGYAVKEASALLFWQHVFALFSLSLYIVIYFKLLSYI 1132

BLAST of Spg009994 vs. TAIR 10
Match: AT1G71090.1 (Auxin efflux carrier family protein )

HSP 1 Score: 578.6 bits (1490), Expect = 4.7e-165
Identity = 302/454 (66.52%), Postives = 366/454 (80.62%), Query Frame = 0

Query: 12  DIRSNEEDILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFT 71
           ++ S   DIL+ +VPL+KL+CLTVIGL+LAHPKT+++P+ TFRLL KLVF LFLPCLIFT
Sbjct: 8   NVNSRVVDILSGVVPLLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLVFALFLPCLIFT 67

Query: 72  ELGQSITLESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGN 131
           ELG+SITL++  QWWFIPVNVLLS  +G  +GYLVV  C PPP+ NR T+++TAFGNTGN
Sbjct: 68  ELGESITLDNIVQWWFIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFTIVMTAFGNTGN 127

Query: 132 LPLAVVGSVCHTSNNPFGPNCHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSE-IED 191
           L LA+V SVCHT  NPFGPNC+S GV+YVS AQW++VIL YT VYHMMEP  E  E +E+
Sbjct: 128 LLLAIVSSVCHTKTNPFGPNCNSRGVSYVSFAQWVAVILVYTVVYHMMEPPLEYYEVVEE 187

Query: 192 EGIKIEEERADG-DIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIK 251
           EG++IEE   +  D  RPLLVEAEWPG+EDKETEH KT  IAR+F+S S  SQ++  ++ 
Sbjct: 188 EGVEIEEINVENHDASRPLLVEAEWPGIEDKETEHCKTPFIARVFNSISSFSQTSFPEVD 247

Query: 252 LSVGDNADSPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIA 311
           L  G+             GGES+ SP+  +CLAEPRV+R++R+VAEQTP++HILQPPTIA
Sbjct: 248 LG-GE------------YGGESS-SPRSIQCLAEPRVMRRIRVVAEQTPVKHILQPPTIA 307

Query: 312 SLVTIIIGLIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILG 371
           SL+ IIIG +PQLKS+ F YDAPLSF+TDSL I+  A VPSVML+LGGML+E PN S LG
Sbjct: 308 SLLAIIIGSVPQLKSVVFGYDAPLSFITDSLNIMGSAMVPSVMLVLGGMLSEGPNESTLG 367

Query: 372 HRTTIGIIIARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIAS 431
            RTTIGI +ARLLVLPL+GIGIV  ADKL  + S DPM++FVLLLQY+TPSAILLGAIAS
Sbjct: 368 LRTTIGISVARLLVLPLVGIGIVMSADKLGLISSADPMFKFVLLLQYSTPSAILLGAIAS 427

Query: 432 LRGYAVREVSALLFWQHIFALFSLSLYVVIYFKI 464
           LRGYAVRE SALLFWQHIFAL SL+ Y+VI+FK+
Sbjct: 428 LRGYAVREASALLFWQHIFALLSLTFYIVIFFKL 447

BLAST of Spg009994 vs. TAIR 10
Match: AT5G01990.1 (Auxin efflux carrier family protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-74
Identity = 168/447 (37.58%), Postives = 254/447 (56.82%), Query Frame = 0

Query: 20  ILAAIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITL 79
           I  A++P+ K+  +  +GL++A     I+P    +LL  LVF L LPCLIF++LGQ++TL
Sbjct: 28  IKIAVMPIAKVFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCLIFSQLGQAVTL 87

Query: 80  ESFAQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGS 139
           +   QWWFIPVNV+L T  G  +G++V +   PP    + T+I    GN GN+PL ++ +
Sbjct: 88  QKMLQWWFIPVNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGNIGNVPLVLLAA 147

Query: 140 VCHTSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEIEDEGIKIEE 199
           +C  ++NPFG +  C   G AY+S  QW+  I+ YT+VY M  P PE  + E+E + ++ 
Sbjct: 148 LCRDTSNPFGDSEKCSIDGTAYISFGQWVGAIILYTYVYQMFAPPPEGFDAEEENLALKT 207

Query: 200 ERADGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIKLSVGDNAD 259
              D    +  L+   +P                + FS T       ST+          
Sbjct: 208 LPVDAAPEQVPLLTQNFP----------------KDFSPTQDLLPVQSTE---------- 267

Query: 260 SPESTDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIG 319
            P    +   G                ++ +    + E+  ++ I+QP  +AS++ +I+G
Sbjct: 268 -PRGRGVSRKG----------------KIAQIFVFLYEKLKLKQIVQPAIVASILAMILG 327

Query: 320 LIPQLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILGHRTTIGII 379
            IP  K L F   APL F TDS  IL  A +P ++L LGG L   P +S LG +TT  II
Sbjct: 328 AIPFTKKLIFTNGAPLFFFTDSCMILGDAMIPCILLALGGNLINGPGSSKLGFKTTAAII 387

Query: 380 IARLLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVRE 439
           I RL+++P +G+GIVT+ADKL FL + D M+RFVLLLQ+T P+++L GA+A+LRG   RE
Sbjct: 388 IGRLVLVPPVGLGIVTVADKLGFLPADDKMFRFVLLLQHTMPTSVLSGAVANLRGCG-RE 430

Query: 440 VSALLFWQHIFALFSLSLYVVIYFKIV 465
            +A+LFW HIFA+FS++ ++V+Y  I+
Sbjct: 448 SAAVLFWVHIFAIFSMAGWMVLYINIL 430

BLAST of Spg009994 vs. TAIR 10
Match: AT2G17500.1 (Auxin efflux carrier family protein )

HSP 1 Score: 186.8 bits (473), Expect = 3.9e-47
Identity = 126/444 (28.38%), Postives = 222/444 (50.00%), Query Frame = 0

Query: 23  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 82
           A +P++++L ++++G  +A  + K+ P E    + K+VFVLF P L+F  L Q++TLE  
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 142
             WWF+PVN+ L+  IG  LG+LVV    PPP L  + V   + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEIEDEGIKIEEERA 202
              +PFG    C + G++Y S +  +     +T+ + +++ S    +  +E  KI  + +
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 203 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIKLSVGDNADSPE 262
           + D+                E +H KT L+                           +PE
Sbjct: 190 NSDL----------------EADH-KTHLLG--------------------------APE 249

Query: 263 STDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 322
                          K  K + E     +  +      ++ +L PPT+ +++  I G + 
Sbjct: 250 D--------------KENKVVKEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVR 309

Query: 323 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILGHRTTIGIIIAR 382
            L++L    DAPL  V  + ++L   T+P + +ILGG L +   +S +     +GI+  R
Sbjct: 310 WLRNLIIGDDAPLRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRSSAVKPMVVLGIVCVR 369

Query: 383 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 442
            + +P++GIGIV  A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S 
Sbjct: 370 YIAMPIIGIGIVLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSV 395

Query: 443 LLFWQHIFALFSLSLYVVIYFKIV 465
           L+ W ++ A+ +L+++  I+  ++
Sbjct: 430 LMLWTYLVAILALTVWSTIFLHLL 395

BLAST of Spg009994 vs. TAIR 10
Match: AT2G17500.3 (Auxin efflux carrier family protein )

HSP 1 Score: 186.8 bits (473), Expect = 3.9e-47
Identity = 126/444 (28.38%), Postives = 222/444 (50.00%), Query Frame = 0

Query: 23  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 82
           A +P++++L ++++G  +A  + K+ P E    + K+VFVLF P L+F  L Q++TLE  
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 142
             WWF+PVN+ L+  IG  LG+LVV    PPP L  + V   + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEIEDEGIKIEEERA 202
              +PFG    C + G++Y S +  +     +T+ + +++ S    +  +E  KI  + +
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 203 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIKLSVGDNADSPE 262
           + D+                E +H KT L+                           +PE
Sbjct: 190 NSDL----------------EADH-KTHLLG--------------------------APE 249

Query: 263 STDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 322
                          K  K + E     +  +      ++ +L PPT+ +++  I G + 
Sbjct: 250 D--------------KENKVVKEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVR 309

Query: 323 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILGHRTTIGIIIAR 382
            L++L    DAPL  V  + ++L   T+P + +ILGG L +   +S +     +GI+  R
Sbjct: 310 WLRNLIIGDDAPLRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRSSAVKPMVVLGIVCVR 369

Query: 383 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 442
            + +P++GIGIV  A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S 
Sbjct: 370 YIAMPIIGIGIVLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSV 395

Query: 443 LLFWQHIFALFSLSLYVVIYFKIV 465
           L+ W ++ A+ +L+++  I+  ++
Sbjct: 430 LMLWTYLVAILALTVWSTIFLHLL 395

BLAST of Spg009994 vs. TAIR 10
Match: AT2G17500.2 (Auxin efflux carrier family protein )

HSP 1 Score: 186.8 bits (473), Expect = 3.9e-47
Identity = 126/444 (28.38%), Postives = 222/444 (50.00%), Query Frame = 0

Query: 23  AIVPLMKLLCLTVIGLVLAHPKTKIIPKETFRLLCKLVFVLFLPCLIFTELGQSITLESF 82
           A +P++++L ++++G  +A  + K+ P E    + K+VFVLF P L+F  L Q++TLE  
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AQWWFIPVNVLLSTAIGCALGYLVVAKCHPPPQLNRMTVIVTAFGNTGNLPLAVVGSVCH 142
             WWF+PVN+ L+  IG  LG+LVV    PPP L  + V   + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TSNNPFGPN--CHSSGVAYVSLAQWISVILAYTFVYHMMEPSPECSEIEDEGIKIEEERA 202
              +PFG    C + G++Y S +  +     +T+ + +++ S    +  +E  KI  + +
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFSMALGGFYIWTYTFRLIKGSAMKVQAIEESEKIAIKSS 189

Query: 203 DGDIGRPLLVEAEWPGVEDKETEHSKTALIARIFSSTSIQSQSTSTDIKLSVGDNADSPE 262
           + D+                E +H KT L+                           +PE
Sbjct: 190 NSDL----------------EADH-KTHLLG--------------------------APE 249

Query: 263 STDIELSGGESTDSPKFTKCLAEPRVVRKMRIVAEQTPIQHILQPPTIASLVTIIIGLIP 322
                          K  K + E     +  +      ++ +L PPT+ +++  I G + 
Sbjct: 250 D--------------KENKVVKEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVR 309

Query: 323 QLKSLFFDYDAPLSFVTDSLEILARATVPSVMLILGGMLAEYPNTSILGHRTTIGIIIAR 382
            L++L    DAPL  V  + ++L   T+P + +ILGG L +   +S +     +GI+  R
Sbjct: 310 WLRNLIIGDDAPLRIVQSTAKLLGDGTIPCMTIILGGNLIQGLRSSAVKPMVVLGIVCVR 369

Query: 383 LLVLPLLGIGIVTLADKLNFLVSGDPMYRFVLLLQYTTPSAILLGAIASLRGYAVREVSA 442
            + +P++GIGIV  A  L FL   DP++++VL+LQ+T P A+ +G +  L   A  E S 
Sbjct: 370 YIAMPIIGIGIVLTAANLGFL-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSV 395

Query: 443 LLFWQHIFALFSLSLYVVIYFKIV 465
           L+ W ++ A+ +L+++  I+  ++
Sbjct: 430 LMLWTYLVAILALTVWSTIFLHLL 395