Spg009970 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009970
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionpullulanase 1, chloroplastic
Locationscaffold7: 5648240 .. 5660749 (-)
RNA-Seq ExpressionSpg009970
SyntenySpg009970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATGACATCACGATCACGTACACATTTGCAGCAGAAAATATCCAACAAACAGTTTCTTTTCCCCTCGCAAGTTCGAAACTCAGTGAAACAGAAAAAATGTCTCTTCTCTTTTCTTCTTCCCTCCATCTCTTACCTTCGTCAACTCCTGTAACTTCCTTCCGTTACATTCTTCCGCCCCGTATCGCCGCCTTTTCTTCATCTCCTCCGCCCGGCGGCCTCTTCTCCGCCGTAGCTGGAACTTCTCCGTCTCGTTATCCCGACGATCACTTTCGCGGTGGACCTCTTTACTGCTCCTCCTCCGCATCAATGTCGCTCGAGGAATCTGCTGCTTCAACTTCTCAGGTTCTATTTCCGTTTCTCACTTGTCGTAATTTGGTATAGGAGACCAATTCTCTGGTTCTATGTTAATCTCTGTCGTAATGTAGTAGGACCGTCTTCAAAGGAAATTTAGATAGGCATCGGCTTAAGTGGTTGAGGTTATTATAATAGCGATCATAACAGAACTCTCTTGGATTACTTCGTTTAGAGGAATAAATACAAATCTAAGAGGAATTTACAGGAAATGAATTGGTCTCCAGGATTTTAGACTACTTGAATTGCTATGCTACATCGTTGCGATTGACTATACGCTGTTTTTTTATGGACATTTCGTTTTTTCGTAAACTGTGAATAGTTGGAAGATAACTTGTTGTACTCGAGAGCTTTTTGGGTGAGCAGATATGTTATAGCGTGGAATGTGGAGGTTGGGAACGGTTCTTGCTATCTGTTTGCCAGTAAAATGGCTACTCTGTGTGTTAAAGGTGGTGTAGTCGAAGGTATGATTATTGTAATAGCTAGACCGTGACTGTGTTGAGATAAAAAAGAGAATGTGCTGATTAATTTTCTTATTGGCAGGCTACGATGCGAAAATCAAGTTGGAAAAAGACTGTGGCCCGCTTACAGAAAATGTACTCAGGAACTAGTAACATTTTAAACTACTTGTAGTCACTAGTCTTTGTGATTTTCGTGTGCATAACCTCCATTTTGTTGTTATGGATATGTATGTATGTATGTATGTATGTATGTATAAAACTGATAGATATCGCTTTATGTTGTTAGGTGATCAAAAAGTTCCCTCATATTCAAAATTACTGCCCTTTCACAGTCCCTCCTGAATCCGACGTTGAAACACTCCTCAAATCTCAACTAGCAGTAGCCACTTTTAACTGTAAATTATCTTCTTTGCTTTGTTTCTCTATCTGTTATCTTATAGCATTCTACTGTAACTAAATAACATATACACAAGCTGCCATCCTCTCTCTTCTGTCTCCTTGATCTTTAGAGTTAAAAGGAAGAAGATAGAAAGAAAAAAAAAAGGAAAAGGGAAAAAGACAGAGTGATTTTGACCGAACATTTGTGGCAGTTGCGTTTATATTCTCGATGAAATGTTTAATGCTGTTGTAAAATTGATGTATTCTGACAAATGCCATTTGTGCAGTGGCATTGTTCCATGTCTTCCTTCCCAAGGATAATATGAAAGGACATGGTCTTTCAAATTATGGATGTTGCTATATTATAGTGCAGTTCCTTATTCAGATACCTTTCATTATTTATTTATTTTTAACCACTGATATATCATCCCTTTCCTTATGGTGTACAGCCTATGGTCAATGCAACAATATTACTTGCCTGCAGCTACCTGGTGTTCTGGATGATTTGTTCTCATATGAAGGTCCTCTAGGTGCAGTTTACTCAAAAGAGGCTGTATCACTTTTCCTTTGGGCTCCAACTGCCCAGGTATACCAATTTCATATTTCACACCAACGTATCCTATTGTCATGATCTGATGTGCATCTTTTGAAATTTAACCTATTCATTCTACTCTCTTTCTGCTGCATCAAGCAGGCTGTACAGGCTCAAATTTACAGGGATCCAGTGGGTGGGATGCCATTTGAAGTTATTCAGCTTGAGGAGCTTGATGGTGTTTGGAGAACGAAAGGACCAAGAAGTTGGAAAGGCTGTTATTATGAATATGAAGTTACAGTCTACCACCCTAGCACCTTGCAAGTTGAAAAATGCTTTACTACTGATCCTTATGCTAGAGGGTATAAACCTCTGGCCCAAAAAATTATGCAACATTGTTTTAGACATGTATACGGACACGTGCAAGAAAGTGCAGGCTTTACATATACTCCACTCTTTTTATTGCTGGAGCAGGGTCTCATCAGATGGAAGGCGGACTTTGTTTGTTGATCTTGGCTCTGATGATTTAAAACCTGAGGGATGGGATAAATTGGCTGATAAGAAACCTCCAGTGAATTCTTTTTCTGACATTAGTATTTATGAGTTGCATATAAGGGATTTTAGGTAAGTACTTTGATTCTTACTGTGTTTGAAGTTCTTTCATGATTTAACTCCAGTTTTTCAGTTAAAACATTGTAGGAAGTGTTGAAAATTATATGCCAAAATTTCCCCTATTCTTGAATGTAGGTGAAAACTGACCTTTACCATTCATGCTGTATTACTATATTACTTTTGTGGAATAAGATATGCTTGACCCAATATTTTATTATCTCTGCCACATAATGAAGCCAAAAACTGACAAGTTTTACCTTCTCATGTTCTTCACTCCATCTTAAAAAGGGGTAGAGAAGGGATGAAAATGGTCAAAAGGTTAAAGAAGTTGATGAGTCTCTTCTGTTAGATGTGGCATTCGTAACAATAATTTTCTGCCTATTTATTCAGAAAATGTTAATTCCAAGCAAAAGAAGTGGAAAATCTAGGATTTTCCTGTGCTATTATTGGGGTGTGTGCATTTCCTTTTCTGATTTAATCATCTCTATCAGCGTCGTGCTGAACTCTTTGCAATTAATGTTCTGTATTGGTAGTGCAGTATAAGTGATCAAAGTGTGCATCCTGATCTGCGTGGTGGTTATATGGCCTTCACTTTGCAGGTACTCCTACTTTTCATCTATTCCGTGAACCACTTATTTGCTGAGTTGCGTTTATTCAACTTAACCAACTTCGTTTCCTTATTTCCTTTAACAAAAAAGGTTTCCTCCCTAGCCTCCCTTATATGCCATGCATGTTTTATCCCTTACCGTCATCATGCATGAATTGGACGTTAGACTAGGATGCTGAAAGTTGTAGGATCCCTCTCTTCTGTCTTCCTTATACGCTAGACATGCATTAGATATCTGGATATCCGAATAAGAAGCTGATCTTGTAGGATCAAATAAAATAATTTTTAACAATAAGCGTATGCCACTTGAAGGATTCTGCTGGCATCACTCATTTAAAGAAGTTATCGAATGCTGGTCTTAGTCATGTTCATCTACTGCCAACCTTCCAATTTGCTGGTGTTGATGATGACAAGAAAAAGTGGAAGTCTCTTGGTAAGTGTATATCTTTGGTTTGCCTTTTGTGATGTTTCAATTCTTGATCCGAAACTGAAAAAGACTGTCTTGGCTATCTTGGTTTTTTGTTTGTCCATCTGAACCCAGTTTCAAGTAGACACGGCTTCGCTGTCAACTTGCCTAAAAAAAAGTAGACATAGCTTTGATATGCAAAATTGTTCCCCTTTTTACTTGCACTTCTTTTGTTTTCAATTGAAAAGTTTTTATCCAAACTTTTTTATCCCCTATTGTGCGAGTACTATCGGAATATTAGGGAACAATGGATGTTTTCTGTAAACTTGGTAGACTGCTTCATAATCCAATCTTTAGTGTATGGAATCAAGTTGCTAATTTCTTACGAATAAATTTTATGGAATTTGTGTGAATTGAGAGATATAGAAGACTTTTTTGAAGATTGGTAGAAATGCCATTAAGGTGAAATTAGGAATATTATTAGAGTATTGATGGTATATTAGTAACTCTTGTAGATTGGCATCGTGAAAAATGACAATCAGAGAGATCCTAAACTCTCCCAAGAACTCAATAATCAAGCTACTTTACTGACACTAGAAGCCCCTGTTTGGCTCTTTTCTTATTCGTTAGGCTAGTCCCTGCTGGGCTCCTTTTAGGCTGTTGTTTTTGTAGCCTGCCTTTGTTTTTTTGGCCAATGTTAGTCCTCTTGTATTCTTTCATTTTTTTTCTCAAGGTTTTTCAATTAGGAAAAAAAATACTATTCAAGCTATTTTCCTCCCTACTTTTTTCCCCTTTCTTATCTATAATTAGTCCCAGAACCCTACCTACTACTACTCACTGTTAGAATTAAGTGTGCCAGTTAGATCCATCCATGAAGCAAAGTGTCAAACTGCCCCTAGCTAACTTACAACAGCCTGCTTCCACCAAATGTGGAGCATTGTCCTGGCTTTTGTTCTTTCTTCTAGTGTCAGTAATGTGTACTTGGGTCCTATCAGTACACGATCTCAAATGAATGGTGTTTCTTAATTCTACATTGAGTTGATTATGGGAGTCCTTGTCGCATTTGATGTGTTTGGCACTCACTGTCTACATAAGGCATGTTGCAGATACCAAACTTCTGGAAAATCTTCCTCCAGATTCAGCTGAACAGCAAGCACTAATTGCAGACATCCAAAACAGTGATGGGTATAACTGGGGGTAAGCTCCTGATTTTGGAGTCTTCTTTGAAGTTGTTTGGTTCTTTGAGTTTTATAGTCTTATTTGTAAATGTCTGTCGTGTCTTTTAGGTACAATCCTATTCTGTGGGGAGTACCTAAAGGAAGCTATGCTAGTGATCCTAATGGTCCTTGTCGTTTAATTGAGTTCCGAAAGATGGTTCAGGTTGACAATTTTGTTTGTGTTTCTGACAAGTCAAGATCAGTATATATTCAGCAATCAGTTACAAGCAGCGCCCAGTTTATCATTTTAAAGTATTAAATGTTTGTTACAGGCACTTAACCAAATTGGCCTTCGTGTTGTGTTGGATGTTGTCTATAATCATCTACATGGACATGGACCTTTTGATCCGAATTCTGTTCTTGATAAGGTCTCAACCTATAATTGCTTTTAATGTACTATAGTACATTTTCACATTTCTTACCTCGTCATTTTCACATTTTCTCAAAGGGGTTTTCTGTTTTCCTTTGTGATAATATGGTTTTCCTCTCCTGTAGATTGTACCAGGTTACTATTTGAGAAGGAACACTGATGGTTTCATTGAGAACAGTACCTGTGTAAATAACACTGCCAGTGAGCATTTTATGGTTGAACGCATGATTGTGGATGATATCTTACATTGGGTAGTTGATTATAAGGTATTTATATTATACAAAAAGATGGAATCTTCAGCTCCTTTATGCAGTTTCATGCAGACAGAACCAGACTATTCATATCTGTCAAACATAATTTCTTGATGTTTATTAGGTTGATGGATTTCGGTTTGATCTTATGGGCCATTTAATGAAAAGTACTATGGTATGTGATTTTATTTTCACTTAAATTCATGCATAGTACTTATAGAGCTTCGTAATTCAAGTGCAACACTCCATCCTCATTATCTGATACTTTGAAACTTCTAATGTTTCAATGACTATCGTGCCAACTTTACATGAATTTTGAATTATGAGTGTAAACTTATGGTCTGTATGAAATCGATGGTTTACCTGAAGTTGAAAGCGAAAGATGCACTACAAAGCTTAACAAAGGAAAAAAATGGAGTTGATGGTTCAAGTATCTACATGTAAGTTTGGGCAAATTTCCTTAGTCCTTACTATTGCACATTGTGTTGGTAGAATAATTCCTGTAATACCACAGATATCAACTTTTTGACACCCATTTTAGTCTACTGAAAATTTAAATCTAATGTTACATTTGAAGATTCTTTTGCCTTCTCTCATATTTCAATCTAGATATGGTGAGGGATGGGACTTTGGGGAAGTCGCAAGAAATGGCCGTGGAGTAAATGCATCACAGTTCAATCTTTTTGGAACTGGACTCGGAAGGTATATACTTTTGTTTCTTGTGCAAGTCTTATGATAGCAGGCAACAAATGTTTCCATGTGCTGAAAAGCATATACCATTGCATGAAAGACATCTAACTTAATTTCACATCCAAAATGTATGATATTATTTTGCCTTTGTAATATATTATTGTTTGTTGGATGCTGGACTTACCAATAAAAGTAACCCTTCTCTATGAAATGTCACATCCAAAGTGAAGGATGGGTTTCATTGCACATCTGCTAAACAATCCTCGAGATGGGCTAACTAGTGGCTGCTCTTGGATGGCAATTTTATCGAAAAATGCAAGATAGCTTTATTTTTCCCATTTTATGTTCAAAGAGATCTTGAAGATGGAGAGACTTTACTATCTTCGGGATCTTGTGCCTTTGTGAATTTTCTTGGGTGATTGAATGAATAAAAACACACCTCATGTTGATGTCTCTGCTGTTTTGTCTCAATTTATAAATGTAACTGTTTGCTAGTTAGGAAATGAGACTTACACTGTTAGCTTGGCACGTGTTTCTTTTGTGCATGAAAAAGTAATGTAATATTTGGAGATTTCAAATTTATCATGTTTTTTCAGTTTTAACGACCGAGTTCGAGATGCAATACTTGGTGGATCTCCATTTGGTCATCCTCTTCAGCAAGGATTCGTGACTGGCCTTTTGCTAGAGGTATTTCAGAATTTCTTTGATCCAGGCGCCTATAATCTAACCTCAACAGTTCGTCACCATGTATCACAAGGGAGGAAAATTGCTCTGATTTATATTTGCTTTAGCAAATTTTAGTTGTAAGACATAGTTTTGTTTTGACTCAGCTATAGGTCTATGATATATGGTCATCAACCCCCTCACTAAGATTGTTGTAATACTGAGCTCTGGTCTAGAGAACTGGTGGGATTTATTTGGAAAGAAAAAATTACCTGTTCAGAGCATTATTTGTGGTGGGGGAGAGCCTTATTGTTGAAGGAGAAGAAAGGGGTTAATATTCACACATTCATTGTTTTTCATTAAACCAGTGTGCATGCATTCGTCCAATCTTTTTACTTGTTTCCAGGATAGGCTGTTGATGATTTTCTTTTCCTTATTTTCTAAAAGAAACAATAAACTTTCTTTTTTAAGAAAAGATGAAAAGTTAAAAGGGTAAATCCCCAAAAAGCTAACTGCAAAAAAAAAAAAGTACAAAAACCTTCAAAATTTATACAAGTATCGTTAAAAATGCTTTTGTTCTTTTCTCTCCATAATTCCCACATTCCTACTATGAGGGCATTTTCCCGTAGGATGTTTGCCCTTCCTTTACAGTTTTGCCCATGCAAAGCTTGTTCCAAGAACTTCTCAAGGGCCAGCTCTAAGAAATATTAAATATACTTAATACTTAGCTCCAGACCTCAGTAGTGAATGCATAATAGAGAAGACGGTTTTAGTTTTACTCATGGCTATTACACATAATACACCAATTTTGGTGTCTAGGTATTAGATATTTTCCTTTGTACTTTGTCATTGGTCTTTAATTTTTCAAAAGGGTCTATAGAAAAATTTTCACCTTTCTATGGAGTTTCTTTGTTCAATTGCCTCAAGTTGAAGTTCCATTAAGAGCATTGCTGAGGGATGGGTTTGCAGGAGAATTTTACCAAGTTATCTCGAGTCCAAACTCTCTTATCAGCATGTTGAGAAGGCATGAGTCCATCTATTTCCTCAACAGGTTCACAAATTCAGCAGCCTCCCATTTCTTAAAGCTCATCTGAACTTTAACTTTCATATTCCCAGCTATCATCCCAATAGTCTACACTCAAAGTATTTTTAGTCTGGGCAGCACCATAAAAACCAGAAGAAAAGAAGTTAAGGGAATCCTCGCAATCTAAAGATCTTCCAAGATCTGAACAGCCTTTTCATTTCTATCCTTGAAGAGGGAGAAATAGTCAGCTGATTTTTTCTGAAGAAGGTTCATGAACTCTAACAGCTTCAGAAGAGTCTATTTACAGAAGTCCATTCCAGGGTTCAATTCCATACTTCCTGACTAAGAGCCATTCTAAAGACAGTGTTTCCTTTGGATTGTTGATGAAATATTAAGTCATGTATTGCTTTCCTTATTCAAATGACTTTTTTTGTTTCATGCTTTTGGTTGAGAAATATGTTTGGTAAATAATTTATATGTTGAATTAAATGTGTTTTTAATTTTTTTTTTCTCTGGCTGTATTGATAATATTTGTTCTTCGTTTGATTATAGTCTTTGTTCCTTATATCCAATATATTCAATTTTTCTTATTTTGCAGCCTAATGATCATGACCATGGAACTGATGAAGTTGCGGAATTCATGCTTGCTGTGTCAAAGGATCATATTCAGGTTAGTCATCTCTGTGTGAGTGGAAGTCACTCAGTCTTTGTATGATCTGTTAGTAGTCCATGTCTTGCTGTAGGAAAAATTTTAGTTAGTGGATACAGATTGTCAGATTATTGTCTTGTCCAGATTGAGGCTAGCACTTGATCGTTTGCATGTTCTAATTGCTCTGATTGACTATATATCCCAGTTTGAGTACTTGCATCGGAAACTTAGATGAAATGCAATTACTGAGTTTTACTAATCTAAATAGATGGATCTTCTTCCAGTATACCATTTCTTCCCCAATTGTTTACATAAGCAATATACTTAATCCTTCGAAAGTTGCATGATTTGTTTGGAGTGTCTTCAAAGTTTTATTTATTTATTTATTTTATTTTTATTTGTTTGTTTCAAATAAATTTCAGGTTGCGATGGCTGCCAATTTGAGGGACTATGTTCTAACCAATTTTGAAGGAAAGGAGGTATTTTCTTTGAAGGGAAGAATGGTTTCTTATTAGAAACTTTTTAAGATGGAATTGGTATTTGTTTTCCAACATATGTATGTACGTACGTCAGTATAAATGTATATTTGCATGTATGTATGTATGTATACGATTCATCCCACTAACTTTATCTAGTTGGTTTTCCAATGTTTTCTTTTTTTCTCAAAAAAAAAAAAGGTTTTCTTTTTTTGAAGGAAAAGTTTTACGGTAAAAACATGGGAAAAGACAGTTATTGGTTTTTCACATCTATGATAATACTCTTGTCTGCTATCCTTTATCAATTAAAACAAGTTACTTTCCCTTTTACATTAAAAAAAAGTATTTTTTAAAGATAACAAATTTTCTCTTAATTATATGGACATATACTTTCTTATGGGCATGGGGGTTATTTTTATTTCCATGAAAAAGGGCGTGCTGTTAAATCAATTATTATTTTCACCTATCCATCCCTAATGGCTTTCACATATGCTGAACATCAGCAAGTACTTTCAATAACCAGAATATCATTTAGCATTTATGATGAATGTTCACAAAGTTTATTTTTTAAAATAAATCAATTATTTTCATGAGAATGACATTGCTTTCCAAATAAATTATAAAGTTTATTATACACTGATTGGTTATGCATTCTAATTTGCATGCTGTAATGGGCATACGTCCGCTTTCTCCTTAAATTTACTCGCGTCCTCTTTACTCAGTCTTTATATTGTAGTACACTTTCTTATCTTAAAGAAATAGAAACAAAAATACGAATGGATCTAATTCATCTGTATGTATATCATTAGTTTTTTCCAACAAGAATTGCATATCAATTTTTTATATGAGAATTTTAGATAATATTATGTCTTTTTCCAGGTGAAGGGGTTAGAAGTACTGACTCACGATGGATCTCCAGTGGCGTATGCTTCTTGCCCTTCAGAAACTGTAAGCTGCACTATCATCTTTAGTTTTGTGAGAATTCATGCAAGTCATCATTAGTTGGCATGTACTTTTTTTTCAATAAGAAGCCATAAAAATATTTACCAACAAAGATCGGTTGAGGAGCTGGTGGGGACGACGGGGTGACCCTTGAGCTTAAGTTGGAGGCCCACCCTTGAGCTTAAGTTGGAGGCCCATGGGCGTGACGGTGGGGGCCGATGGGGTTGTAGAGGAAGGAGAGAGGGAAGGGAGAGGGAGGGATAGGGTGAAGCGGAAGGGAGAGAGGGAGAGATTGGTTTTTAAAATTTTTGCCATGTCATCTTTAAAATATTTTACGAATAATTATTTCTTGACGTGAAATATTAACATCAATTAAAAAATATAAAGTCAAAACTATCACATTCACACTAATCGAAGGGACGAAAATAGAAAGTTGAGGAGCTGGCAGACCCAGTAGAATATGTTACAGGCACACTTTTAATTGCTTTGGAGTTCTAGAAACCCGTTAAGGCGAGAATTAGGGGAAATGGGCAAAATAGAAGAAGAGAACTATTGTCTTCCCAGTTCTCAAAGGTGGATGGCCTTAGCTTATATATAGGCGACGTTATAAGTTTAGCTACTTAATTCATGCCATATTTTCAATGCCATATTGTGGTTAATTGAATCTGGATTATGTTCAGTGGGTGGATCATTGGATGTCTACATATAAGAAAGGAAGTATAGGATGCCTTTGGTATTCTATTATAAAGCTAAAATCTTTAATTGACAGTAATTCTTCCTTGAAACTTGGGAATGGAAGAAAATCAGATTTTGGCTTGGATAAATGGCTAGACGACCAGGGTCCTGAAGGACAAATCCGAGGATCTACAGAGATCAAAGGAAAATTTTATATATTTCTTCAACGAAGCAAAGAGGCCCAAAGTCTCTTGGAAAAAAGCTGTAGAGGGAGAAACTAACAAAAAAGAACTATCAGCATTAATTTCACTTAACACAAGGATTAAATCTATCCGGTACTTGAACTTTTAAGATCTTGAGGAGTTTTTTGCCAATTTCAATTTTTTCCTAGAATAATTGTCGAAACCAGCATTATCATGTATCAATGCTCACATCGTCTTGGATATTAAGATATTAATTTAAATTCCGATAAGCACAGCCATGCATTTGACATTCTCAATCGATTTCCAGGTGAATTATGCTTCCGCTCACGATAATGAAACCCTATTCGACGTTGTGAGTTTGAAGGTATTGTGAACGTGGTTTTGTGAATCATACTGCTTTCTTAAAGAGTTGACTTCTGATTATAGTCCTTTTCTTGTAGACTCCGATAAACATTACCGTGAATGACAGGTGCAGGATTAATCATTTGGCAACCAGTATAATAGCACTATCGCAGGTATTTAATTTTAATTAATCATGAAAGTTGGTAGTCTTCATCTTTACGATTGTTAATTCTTCTATTATCTACAAAGTTCAAGGGTGACATGTGCAAATCAGAAGACAGGAAACCATGTTCATGTGCCTCAATAAACCACCAACAATCCTGGAAGTTAAAGTTATACAGATATGGCTACTTGAATCCTATTATTCTGAACGTTTAACATCCCTAGCTAGTGACCTGTGCTTATCTGCATGAGTTTGTAAATGACACTAAACTTGGTATTGTTCTGTATAGAACCAACAGATTAAACTAGATATGGAAATTGCATCTGGAAGTTACCTAGACTAGAACTGAGGAACTCATGCAAAATTCATAGCGTTAACCAAGTCTCTACTGATACAGGACATGGATGCCGGATGCATGACATACATTTTTACATGTTCTAGGAGTTCTTTATAATTGTTTTTTTTTTTGGAAGAGAAACAAATTTCATTGATGAATGAAATGTGGAGAAAAACTCCGGACACCCAAGGGTGAGTTACATAAATGCTTTCCAATTGGAAATTAAAAAGGATAAACTATAGTCAGAGAAAGGGTGTTTACATTTACACCAATACAATGCATGAAAAAGAATTAGATCCATAAATTTATCAAAGGAAGAGAAAGAATCCCTGAAAATTCGAGAATTTCGTTCGCCCCATAAAAACCAGAAGAAAACTCTGTTCAAAGCAAGCCACAAGATCTTCTTTTCTCCACGAAAGGGATGATCCACAAAAATGAGAGTAAGAACATCCTTAATGCAATTTGGAAAAACGATGGACCATCCAAAAGCATCAAGAATCTCTGACCAATATCTGGAAGCGAAGGTACAATGGACAAATAGATGCCTAGGATGTTCTGAACTAGCAGCACACATTATGCACCAAGATGGAGACAAATGAAAATGAGGCATCCGTCGTTGAAGACGATCAGCTGTATTAATAGCACCATGACTGAGTTCCCATAAAAAAATCTTGATCTTCTTTGGATAGAAATCTGACCAAATGACCTTATATAGATCATTTGCCATATTCGGACGGTCTACTAAGTCTTCCATGAGAGATTTAACAGAAAAAATATTGGACGATTCCAAAGGCCAAATCCAGGAATCATTACGGTTCTGTAATCTGATGGAGGAAAGAATAAGTGATAAGTCCATCCATTCCTCTGTCTCTATATCATTTAAATTACGCCGAAGACTCAGATCCCATGCTGATTGAGTAGCAATCCATGTTTCACCAACCAAACTCCTCGGACGATCTGTTAAACGATAAAGGCGAGGAAAATTTGTAGCCAGAACACCACAACTTAACCACGAATCATGCCAAAATGATGTAGCAAGACCATTACCCAATCTTCTTTTTACACGTGAAGATACAAGGTCAATAGTGGAAGTAATAAATCGCCAAGGAGATTTGTGAGAACTTTTCTGAATAATGCTAGGCCAAAGACTAGGCAACTCA

mRNA sequence

ATGTCTCTTCTCTTTTCTTCTTCCCTCCATCTCTTACCTTCGTCAACTCCTGTAACTTCCTTCCGTTACATTCTTCCGCCCCGTATCGCCGCCTTTTCTTCATCTCCTCCGCCCGGCGGCCTCTTCTCCGCCGTAGCTGGAACTTCTCCGTCTCGTTATCCCGACGATCACTTTCGCGGTGGACCTCTTTACTGCTCCTCCTCCGCATCAATGTCGCTCGAGGAATCTGCTGCTTCAACTTCTCAGTTGGAAGATAACTTGTTGTACTCGAGAGCTTTTTGGGTGAGCAGATATGTTATAGCGTGGAATGTGGAGGTTGGGAACGGTTCTTGCTATCTGTTTGCCAGTAAAATGGCTACTCTGTGTGTTAAAGGTGGTGTAGTCGAAGGCTACGATGCGAAAATCAAGTTGGAAAAAGACTGTGGCCCGCTTACAGAAAATGTGATCAAAAAGTTCCCTCATATTCAAAATTACTGCCCTTTCACAGTCCCTCCTGAATCCGACGTTGAAACACTCCTCAAATCTCAACTAGCAGTAGCCACTTTTAACTGTAAATTATCTTCTTTGCTTTGTTTCTCTATCTCCTATGGTCAATGCAACAATATTACTTGCCTGCAGCTACCTGGTGTTCTGGATGATTTGTTCTCATATGAAGGTCCTCTAGGTGCAGTTTACTCAAAAGAGGCTGTATCACTTTTCCTTTGGGCTCCAACTGCCCAGGCTGTACAGGCTCAAATTTACAGGGATCCAGTGGGTGGGATGCCATTTGAAGTTATTCAGCTTGAGGAGCTTGATGGTGTTTGGAGAACGAAAGGACCAAGAAGTTGGAAAGGCTGTTATTATGAATATGAAGTTACAGTCTACCACCCTAGCACCTTGCAAGTTGAAAAATGCTTTACTACTGATCCTTATGCTAGAGGGGTCTCATCAGATGGAAGGCGGACTTTGTTTGTTGATCTTGGCTCTGATGATTTAAAACCTGAGGGATGGGATAAATTGGCTGATAAGAAACCTCCAGTGAATTCTTTTTCTGACATTAGTATTTATGAGTTGCATATAAGGGATTTTAGTATAAGTGATCAAAGTGTGCATCCTGATCTGCGTGGTGGTTATATGGCCTTCACTTTGCAGGATTCTGCTGGCATCACTCATTTAAAGAAGTTATCGAATGCTGGTCTTAGTCATGTTCATCTACTGCCAACCTTCCAATTTGCTGGTGTTGATGATGACAAGAAAAAGTGGAAGTCTCTTGATACCAAACTTCTGGAAAATCTTCCTCCAGATTCAGCTGAACAGCAAGCACTAATTGCAGACATCCAAAACAGTGATGGGTATAACTGGGGGTACAATCCTATTCTGTGGGGAGTACCTAAAGGAAGCTATGCTAGTGATCCTAATGGTCCTTGTCGTTTAATTGAGTTCCGAAAGATGGTTCAGGCACTTAACCAAATTGGCCTTCGTGTTGTGTTGGATGTTGTCTATAATCATCTACATGGACATGGACCTTTTGATCCGAATTCTGTTCTTGATAAGATTGTACCAGGTTACTATTTGAGAAGGAACACTGATGGTTTCATTGAGAACAGTACCTGTGTAAATAACACTGCCAGTGAGCATTTTATGGTTGAACGCATGATTGTGGATGATATCTTACATTGGGTAGTTGATTATAAGGTTGATGGATTTCGGTTTGATCTTATGGGCCATTTAATGAAAAGTACTATGTTGAAAGCGAAAGATGCACTACAAAGCTTAACAAAGGAAAAAAATGGAGTTGATGGTTCAAGTATCTACATATATGGTGAGGGATGGGACTTTGGGGAAGTCGCAAGAAATGGCCGTGGAGTAAATGCATCACAGTTCAATCTTTTTGGAACTGGACTCGGAAGTTTTAACGACCGAGTTCGAGATGCAATACTTGGTGGATCTCCATTTGGTCATCCTCTTCAGCAAGGATTCGTGACTGGCCTTTTGCTAGAGCCTAATGATCATGACCATGGAACTGATGAAGTTGCGGAATTCATGCTTGCTGTGTCAAAGGATCATATTCAGGTTGCGATGGCTGCCAATTTGAGGGACTATGTTCTAACCAATTTTGAAGGAAAGGAGGTGAAGGGGTTAGAAGTACTGACTCACGATGGATCTCCAGTGGCGTATGCTTCTTGCCCTTCAGAAACTGTGAATTATGCTTCCGCTCACGATAATGAAACCCTATTCGACGTTGTGAGTTTGAAGACTCCGATAAACATTACCGTGAATGACAGGTGCAGGATTAATCATTTGGCAACCAGTATAATAGCACTATCGCAGGTATTTAATTTTAATTAA

Coding sequence (CDS)

ATGTCTCTTCTCTTTTCTTCTTCCCTCCATCTCTTACCTTCGTCAACTCCTGTAACTTCCTTCCGTTACATTCTTCCGCCCCGTATCGCCGCCTTTTCTTCATCTCCTCCGCCCGGCGGCCTCTTCTCCGCCGTAGCTGGAACTTCTCCGTCTCGTTATCCCGACGATCACTTTCGCGGTGGACCTCTTTACTGCTCCTCCTCCGCATCAATGTCGCTCGAGGAATCTGCTGCTTCAACTTCTCAGTTGGAAGATAACTTGTTGTACTCGAGAGCTTTTTGGGTGAGCAGATATGTTATAGCGTGGAATGTGGAGGTTGGGAACGGTTCTTGCTATCTGTTTGCCAGTAAAATGGCTACTCTGTGTGTTAAAGGTGGTGTAGTCGAAGGCTACGATGCGAAAATCAAGTTGGAAAAAGACTGTGGCCCGCTTACAGAAAATGTGATCAAAAAGTTCCCTCATATTCAAAATTACTGCCCTTTCACAGTCCCTCCTGAATCCGACGTTGAAACACTCCTCAAATCTCAACTAGCAGTAGCCACTTTTAACTGTAAATTATCTTCTTTGCTTTGTTTCTCTATCTCCTATGGTCAATGCAACAATATTACTTGCCTGCAGCTACCTGGTGTTCTGGATGATTTGTTCTCATATGAAGGTCCTCTAGGTGCAGTTTACTCAAAAGAGGCTGTATCACTTTTCCTTTGGGCTCCAACTGCCCAGGCTGTACAGGCTCAAATTTACAGGGATCCAGTGGGTGGGATGCCATTTGAAGTTATTCAGCTTGAGGAGCTTGATGGTGTTTGGAGAACGAAAGGACCAAGAAGTTGGAAAGGCTGTTATTATGAATATGAAGTTACAGTCTACCACCCTAGCACCTTGCAAGTTGAAAAATGCTTTACTACTGATCCTTATGCTAGAGGGGTCTCATCAGATGGAAGGCGGACTTTGTTTGTTGATCTTGGCTCTGATGATTTAAAACCTGAGGGATGGGATAAATTGGCTGATAAGAAACCTCCAGTGAATTCTTTTTCTGACATTAGTATTTATGAGTTGCATATAAGGGATTTTAGTATAAGTGATCAAAGTGTGCATCCTGATCTGCGTGGTGGTTATATGGCCTTCACTTTGCAGGATTCTGCTGGCATCACTCATTTAAAGAAGTTATCGAATGCTGGTCTTAGTCATGTTCATCTACTGCCAACCTTCCAATTTGCTGGTGTTGATGATGACAAGAAAAAGTGGAAGTCTCTTGATACCAAACTTCTGGAAAATCTTCCTCCAGATTCAGCTGAACAGCAAGCACTAATTGCAGACATCCAAAACAGTGATGGGTATAACTGGGGGTACAATCCTATTCTGTGGGGAGTACCTAAAGGAAGCTATGCTAGTGATCCTAATGGTCCTTGTCGTTTAATTGAGTTCCGAAAGATGGTTCAGGCACTTAACCAAATTGGCCTTCGTGTTGTGTTGGATGTTGTCTATAATCATCTACATGGACATGGACCTTTTGATCCGAATTCTGTTCTTGATAAGATTGTACCAGGTTACTATTTGAGAAGGAACACTGATGGTTTCATTGAGAACAGTACCTGTGTAAATAACACTGCCAGTGAGCATTTTATGGTTGAACGCATGATTGTGGATGATATCTTACATTGGGTAGTTGATTATAAGGTTGATGGATTTCGGTTTGATCTTATGGGCCATTTAATGAAAAGTACTATGTTGAAAGCGAAAGATGCACTACAAAGCTTAACAAAGGAAAAAAATGGAGTTGATGGTTCAAGTATCTACATATATGGTGAGGGATGGGACTTTGGGGAAGTCGCAAGAAATGGCCGTGGAGTAAATGCATCACAGTTCAATCTTTTTGGAACTGGACTCGGAAGTTTTAACGACCGAGTTCGAGATGCAATACTTGGTGGATCTCCATTTGGTCATCCTCTTCAGCAAGGATTCGTGACTGGCCTTTTGCTAGAGCCTAATGATCATGACCATGGAACTGATGAAGTTGCGGAATTCATGCTTGCTGTGTCAAAGGATCATATTCAGGTTGCGATGGCTGCCAATTTGAGGGACTATGTTCTAACCAATTTTGAAGGAAAGGAGGTGAAGGGGTTAGAAGTACTGACTCACGATGGATCTCCAGTGGCGTATGCTTCTTGCCCTTCAGAAACTGTGAATTATGCTTCCGCTCACGATAATGAAACCCTATTCGACGTTGTGAGTTTGAAGACTCCGATAAACATTACCGTGAATGACAGGTGCAGGATTAATCATTTGGCAACCAGTATAATAGCACTATCGCAGGTATTTAATTTTAATTAA

Protein sequence

MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRGGPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVATFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQAVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKLLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNFN
Homology
[Close]
BLAST of Spg009970 vs. NCBI nr
Match: XP_038892369.1 (pullulanase 1, chloroplastic [Benincasa hispida] >XP_038892370.1 pullulanase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1390.9 bits (3599), Expect = 0.0e+00
Identity = 680/774 (87.86%), Postives = 718/774 (92.76%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MSLLFSSSLHLLPSSTP TS  Y   PRIAAFSSSPP GG FS V+ TS S + DD+FR 
Sbjct: 1   MSLLFSSSLHLLPSSTPATSICYQAHPRIAAFSSSPPAGGFFS-VSATSLSHFLDDNFRS 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           G LYCSSS+SMSLEES  STSQLED+LLYSRAFWVSRYVIAWNVEVG+GSCYLFASKMAT
Sbjct: 61  GSLYCSSSSSMSLEES-TSTSQLEDSLLYSRAFWVSRYVIAWNVEVGSGSCYLFASKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L ++ GVVEGYD K+KLEKD G L ENVIKKFPHIQNYCPFTVP  SDVE LLK QLAVA
Sbjct: 121 LRIEDGVVEGYDVKLKLEKDRGRLPENVIKKFPHIQNYCPFTVPLASDVEALLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TFN           SYG+CNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSL+LWAPTAQ
Sbjct: 181 TFN-----------SYGECNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AV+AQI+RDPVGG PFEVIQLEELDGVWRTKGP+SW+GCYYEYEVTVYHPSTLQ+EKCFT
Sbjct: 241 AVRAQIFRDPVGGTPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQIEKCFT 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RG+SSDGRRTLFVD+GS+DL P+GWDKLAD+K PV+SFSDISIYELH+RDFSISD
Sbjct: 301 TDPYSRGISSDGRRTLFVDIGSNDLIPKGWDKLADEKHPVDSFSDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPDLRGG+MAFTLQDSAGI HLKKLSNAG+SHVHLLPTFQF GVDDDK KWK  DT+
Sbjct: 361 QSVHPDLRGGFMAFTLQDSAGINHLKKLSNAGISHVHLLPTFQFGGVDDDKTKWKFFDTE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLE+LPPDSAEQ++LIADI NSDGYNWGYNPILWGVPKGSYASDPNGPCR+IEFRKMVQA
Sbjct: 421 LLEDLPPDSAEQESLIADILNSDGYNWGYNPILWGVPKGSYASDPNGPCRVIEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNADGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQ LTKEKNGVDGSSIYIY
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDHDHGTD+VAE MLAVSKDHI+VAMAANLRDYVLTNFEG+EVKGLEVLTHDGSPVAYA
Sbjct: 661 PNDHDHGTDKVAESMLAVSKDHIEVAMAANLRDYVLTNFEGQEVKGLEVLTHDGSPVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCPSETVNY SAHDNETLFD+VSLKTP NI V+DRCRINHLATSIIALSQ   F
Sbjct: 721 SCPSETVNYVSAHDNETLFDIVSLKTPRNIAVDDRCRINHLATSIIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. NCBI nr
Match: XP_023542048.1 (pullulanase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 676/774 (87.34%), Postives = 710/774 (91.73%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MS LFSSSLHLLP S P TSF + LPPRIAA  SS P GG+FS +A TS S YPD H R 
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFS-LAATSLSHYPDGHSRC 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           GPLYCSSSASMS+EES  S SQLED+LLY+RAFWVSRYVIAWNVEVG+GSCYLF SKMAT
Sbjct: 61  GPLYCSSSASMSVEES-TSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L VK GVVEGYD KIKLEKD GPL ENVIKKFPHIQNYCPFTVP  S VE LLK QLAVA
Sbjct: 121 LRVKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TFN           SYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSL+LWAPTAQ
Sbjct: 181 TFN-----------SYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AVQAQIY++PVGG PFEVIQLEELDGVWRTKGP+SW+GCYYEYEVTVYHPSTL+VEKCF+
Sbjct: 241 AVQAQIYKEPVGGRPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFS 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RGVSSDGRRTLFVDLGSDDLKPEGWDKLAD+KPP++SFSDISIYELH+RDFSISD
Sbjct: 301 TDPYSRGVSSDGRRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPDLRGGYM FTLQDS GITHLKKLSNAG+SHVHLLPTFQF GVDDDK KWKSLD +
Sbjct: 361 QSVHPDLRGGYMGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLE LPPDSAEQQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA
Sbjct: 421 LLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIY+Y
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDHDHG+D+  EF LAVSKDHIQVAMAANLRDYVLTNFEGKEVKG EV T+DGS VAYA
Sbjct: 661 PNDHDHGSDKEVEFTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCP+ETVNY SAHDNETLFDVVSLKTP NITVNDRCR+NHLATSIIALSQ   F
Sbjct: 721 SCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. NCBI nr
Match: XP_022945721.1 (pullulanase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1373.2 bits (3553), Expect = 0.0e+00
Identity = 675/774 (87.21%), Postives = 709/774 (91.60%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MS LFSSSLHLLP S P TSF + LPPRIAA SSSP  GG+FS VA TS S YP  H R 
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFS-VAATSLSHYPHGHSRC 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           GPLYCSSSASMSLEES  + SQLED+LLY+RAFWVSRYVIAWNVEVG+GSCYLF SKMAT
Sbjct: 61  GPLYCSSSASMSLEESTPA-SQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L V  GVVEGYD KIKLEKD GPL ENVIKKFPHIQNYCPFTVP  S VE LLK QLAVA
Sbjct: 121 LRVNDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TFN           SYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSL+LWAPTAQ
Sbjct: 181 TFN-----------SYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AVQAQIY++PVGG PFEVIQLEELDGVWRTKGP+SW+GCYYEYEVTVYHPSTL+VEKCF+
Sbjct: 241 AVQAQIYKEPVGGRPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFS 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RGVSSDGRRTLFVDLGSDDLKPEGWDKLAD+KPP++SFSDISIYELH+RDFSISD
Sbjct: 301 TDPYSRGVSSDGRRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPD+RGGYM FTLQDS GITHLKKLSNAG+SHVHLLPTFQF GVDDDK KWKSLD +
Sbjct: 361 QSVHPDMRGGYMGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLE LPPDSAEQQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA
Sbjct: 421 LLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIY+Y
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDHDHGTD+ AEF LAVSKDHIQVAMAANL+DYVL NFEGKEVKG EV T+DGS VAYA
Sbjct: 661 PNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCP+ETVNY SAHDNETLFDVVSLKTP NITVNDRCR+NHLATSIIALSQ   F
Sbjct: 721 SCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. NCBI nr
Match: KAG6573853.1 (Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1370.5 bits (3546), Expect = 0.0e+00
Identity = 674/774 (87.08%), Postives = 707/774 (91.34%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MS LFSSSLHLLP S P TSF + LPPRIAA SSSP  GG+FS VA TS S YPD H R 
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFS-VAATSLSHYPDGHSRC 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           GPLYCSSSASMSLEES  + SQLED+LLY+RAFWVSRYVIAWNVEV +GSCYLF SKMAT
Sbjct: 61  GPLYCSSSASMSLEESTPA-SQLEDSLLYARAFWVSRYVIAWNVEVRSGSCYLFGSKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L V  GVVEGYD KIKLEKD GPL ENVIKKFPHIQNYCPFTVP  S VE LLK QLAVA
Sbjct: 121 LRVNDGVVEGYDVKIKLEKDSGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TFN           SYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKE VSL+LWAPTAQ
Sbjct: 181 TFN-----------SYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEVVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AVQAQIY++PVGG PFEVIQLEELDGVWRTKGP+SW+GCYYEYEVTVYHPSTL+VEKCF+
Sbjct: 241 AVQAQIYKEPVGGRPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFS 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RGVSSDGRRTLFVDLGSDDLKPEGWDKLAD+KPP++SFSDISIYELH+RDFSISD
Sbjct: 301 TDPYSRGVSSDGRRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPD+RGGYM FTLQDS GITHLKKLSNAG+SHVHLLPTFQF GVDDDK KWKSLD +
Sbjct: 361 QSVHPDMRGGYMGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLE LPPDSAEQQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA
Sbjct: 421 LLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLHG GPFDP SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHGCGPFDPISVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIY+Y
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDHDHGTD+ AEF LAVSKDHIQVAMAANL+DYVL NFEGKEVKG EV T+DGS VAYA
Sbjct: 661 PNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCP+ETVNY SAHDNETLFDVVSLKTP NITVNDRCR+NHLATSIIALSQ   F
Sbjct: 721 SCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. NCBI nr
Match: XP_008446816.1 (PREDICTED: pullulanase 1, chloroplastic [Cucumis melo] >KAA0034691.1 pullulanase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1357.0 bits (3511), Expect = 0.0e+00
Identity = 671/774 (86.69%), Postives = 700/774 (90.44%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MSLLFSSSLH  PS    TS RY L PR AA SSSPP  GLFSA AGTS S     +FR 
Sbjct: 1   MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSA-AGTSLSHGSHHNFRT 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           GPLYCS S+SMSLEES  STSQLED+LLYSRAFWVSR+VIAWNVEV +GSCYLFASKMAT
Sbjct: 61  GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L VK GVVEGYD KI LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121 LRVKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TF+           SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSL+LWAPTAQ
Sbjct: 181 TFD-----------SYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AV+AQI+RDPVGGMPFEVI LEE+DGVWRTKGP+SW+GCYYEYEVTVYHPSTLQVEKCFT
Sbjct: 241 AVRAQIFRDPVGGMPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFT 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RGVSSDGRRTLFVDL SDDL P+GWDKLAD KPPV+SF+DISIYELH+RDFSISD
Sbjct: 301 TDPYSRGVSSDGRRTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPDLRGGYMAFTLQDSAGI HLK LSNAGLSHVHLLPTF F GVDDDK KWK +DT+
Sbjct: 361 QSVHPDLRGGYMAFTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLENLPPDSAEQQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKMVQA
Sbjct: 421 LLENLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLH HGP D NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQ LTKEKNGVDGSSIYIY
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDH HGT EVAE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYA
Sbjct: 661 PNDHHHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCP+ETVNY SAHDNETLFD+VSLKT  NITV+DRCRINHLATSIIALSQ   F
Sbjct: 721 SCPTETVNYVSAHDNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. ExPASy Swiss-Prot
Match: Q8GTR4 (Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2)

HSP 1 Score: 1008.1 bits (2605), Expect = 5.6e-293
Identity = 476/709 (67.14%), Postives = 577/709 (81.38%), Query Frame = 0 

Query: 66  SSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLCVKG 125
           S+S+ MSLE S+ + SQ  D L+YSRA+WV++ VIAWNV+VG GSCY +ASK A L    
Sbjct: 65  SASSPMSLEVSSPN-SQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSE 124

Query: 126 GVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVATFNCK 185
             ++GYD +IKLE + G L  +VI+KFPHI+NY  F VP + D+  L+KSQLAV  F+ +
Sbjct: 125 DGIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAE 184

Query: 186 LSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQAVQAQ 245
                      G+    T LQLPGVLD+LFSY+GPLGA ++ E VSL LWAPTAQAV   
Sbjct: 185 -----------GRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVC 244

Query: 246 IYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYA 305
           IY++P+   P E+  L+E +GVW T+G  SW GCYY Y+V+VYHPST+++E C+  DPYA
Sbjct: 245 IYKNPLDKSPMEICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYA 304

Query: 306 RGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHP 365
           RG+S+DGR+T  V+L SDDLKPEGWD LADKKP + SFSDISIYELH+RDFS +D++V P
Sbjct: 305 RGLSADGRKTFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEP 364

Query: 366 DLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKLLENL 425
           + RGGY+AFT +DSAG+ HL+KL +AGL+H+HLLPTFQF  VDD+K+ WKS+DT LLE L
Sbjct: 365 ENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGL 424

Query: 426 PPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIG 485
            PDS E QA I +IQN DGYNWGYNP+LWGVPKGSYASDP GPCR+IEFRKMVQALN  G
Sbjct: 425 RPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTG 484

Query: 486 LRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERM 545
           L VVLDVVYNHLH  GP D  SVLDKIVPGYYLRRN+DGFIENSTCVNNTASEH+MV+R+
Sbjct: 485 LNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRL 544

Query: 546 IVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWD 605
           I DD+L+WVV+YKVDGFRFDLMGH+MK+T++ AK A+ SL KE +GVDGS IY+YGEGW+
Sbjct: 545 IRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWN 604

Query: 606 FGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHD 665
           FGEVA NGRG+NASQFNL GTG+GSFNDR+RDA LGGSPFGHPLQQGF+TGLLL+PN HD
Sbjct: 605 FGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHD 664

Query: 666 HGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPSE 725
           HG++   E ML+ +K+HIQ  MAANL+DY+LTN EGKEVKG EVL HD +PVAYAS P+E
Sbjct: 665 HGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTE 724

Query: 726 TVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           T+NY SAHDNETLFD++SLKTP+ I+V++RCRINHLA+S+IALSQ   F
Sbjct: 725 TINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. ExPASy Swiss-Prot
Match: P07206 (Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2)

HSP 1 Score: 422.5 bits (1085), Expect = 1.0e-116
Identity = 257/755 (34.04%), Postives = 400/755 (52.98%), Query Frame = 0 

Query: 68  SASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLCVKGGV 127
           + + ++ +S A   +    +  + A WV +  + W          L+ S  + +   G  
Sbjct: 162 AGNSAVYDSRADAFRAAFGVALAEAHWVDKNTLLWPGGQDKPIVRLYYSHSSKVAADG-- 221

Query: 128 VEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQ-LAVATFNCKL 187
            EG      L+     +++ V  +FPH+ +Y  F +P  ++V+ LL+ + +A+A      
Sbjct: 222 -EGKFTDRYLKLTPTTVSQQVSMRFPHLSSYAAFKLPDNANVDELLQGETVAIA------ 281

Query: 188 SSLLCFSISYGQCNNITCLQLPGVLDDLFSYEG---PLGAVYSKEAVSLFLWAPTAQAVQ 247
                 +   G   + T +Q  GVLDD ++        GA  +   V+  +WAPTAQ V 
Sbjct: 282 ------AAEDGILISATQVQTAGVLDDAYAEAAEALSYGAQLADGGVTFRVWAPTAQQVD 341

Query: 248 AQIY---RDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 307
             +Y   +  +G  P   +  +   G W  +G    KG +Y Y +TVYHP + +VE+   
Sbjct: 342 VVVYSADKKVIGSHP---MTRDSASGAWSWQGGSDLKGAFYRYAMTVYHPQSRKVEQYEV 401

Query: 308 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLA--DKKPPVNSFSDISIYELHIRDFSI 367
           TDPYA  +S++   +  VDL    LKP+GWD L     +      + ++I+E HIRD S 
Sbjct: 402 TDPYAHSLSTNSEYSQVVDLNDSALKPDGWDNLTMPHAQKTKADLAKMTIHESHIRDLSA 461

Query: 368 SDQSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTF---------------- 427
            DQ+V  +LRG Y+A T  DS  + HLK LS +G++HV LLP F                
Sbjct: 462 WDQTVPAELRGKYLALTAGDSNMVQHLKTLSASGVTHVELLPVFDLATVNEFSDKVADIQ 521

Query: 428 ----------------QFAGVDDDKKKWKSLDTKLLENLPPDSAEQQALIADIQNSDGYN 487
                           +FAG  D     + +  +L ++   D+ + QAL   +  +D YN
Sbjct: 522 QPFSRLCEVNSAVKSSEFAGYCDSGSTVEEVLNQLKQSDSQDNPQVQALNTLVAQTDSYN 581

Query: 488 WGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQ-IGLRVVLDVVYNHLHGHGPFDP 547
           WGY+P  + VP+GSYA+DP G  R+ EFR M+QA+ Q +G+ V++DVVYNH +  GP D 
Sbjct: 582 WGYDPFHYTVPEGSYATDPEGTTRIKEFRTMIQAIKQDLGMNVIMDVVYNHTNAAGPTDR 641

Query: 548 NSVLDKIVPGYYLRRN-TDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRF 607
            SVLDKIVP YY R N T G +E++TC +++A EH M  ++I D +  W  DYK+DGFRF
Sbjct: 642 TSVLDKIVPWYYQRLNETTGSVESATCCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFRF 701

Query: 608 DLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVARNGRGVNASQFNLF 667
           DLMG+  K+ +L A + +++L  +        IY +GEGWD  +   + R   ASQ NL 
Sbjct: 702 DLMGYHPKAQILSAWERIKALNPD--------IYFFGEGWDSNQ---SDRFEIASQINLK 761

Query: 668 GTGLGSFNDRVRDAILGGSPF--GHPLQ--QGFVTGLLLEPNDHDHGTDEVAEFMLAVSK 727
           GTG+G+F+DR+RD++ GG PF  G  L+  QG  +G  + PN+    +D+    +     
Sbjct: 762 GTGIGTFSDRLRDSVRGGGPFDSGDALRQNQGIGSGAGVLPNELASLSDDQVRHL----A 821

Query: 728 DHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPSETVNYASAHDNETLFD 776
           D  ++ MA NL D+V+ + +G   KG E+  ++G+P  YA+ P+E VNY S HDN+TL+D
Sbjct: 822 DLTRLGMAGNLADFVMIDKDGAAKKGSEI-DYNGAPGGYAADPTEVVNYVSKHDNQTLWD 881

[Close]
BLAST of Spg009970 vs. ExPASy Swiss-Prot
Match: P07811 (Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 5.5e-115
Identity = 255/748 (34.09%), Postives = 394/748 (52.67%), Query Frame = 0 

Query: 68  SASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLCVKGGV 127
           + + ++ +S A   +    +  + A WV +  + W          L+ S  + +      
Sbjct: 173 AGNSAVYDSRADAFRAAFGVALADAHWVDKTTLLWPGGENKPIVRLYYSHSSKVAADSN- 232

Query: 128 VEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVATFNCKLS 187
            E  D  +KL      + + V  +FPH+ +Y  F +P + +V+ LL+        +  + 
Sbjct: 233 GEFSDKYVKLTPT--TVNQQVSMRFPHLASYPAFKLPDDVNVDELLQGDDGGIAESDGIL 292

Query: 188 SLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQAVQAQIY 247
           SL        +     C +      +  SY    GA  +   V+  +WAPTAQ V+  IY
Sbjct: 293 SLSHPGADRRRAGRYLCRRA-----EALSY----GAQLTDSGVTFRVWAPTAQQVELVIY 352

Query: 248 RDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYARG 307
                 +    +  +   G W  +G    KG +Y Y +TVYHP + +VE+   TDPYA  
Sbjct: 353 SADKKVIASHPMTRDSASGAWSWQGGSDLKGAFYRYAMTVYHPQSRKVEQYEVTDPYAHS 412

Query: 308 VSSDGRRTLFVDLGSDDLKPEGWDKLA--DKKPPVNSFSDISIYELHIRDFSISDQSVHP 367
           +S++   +  VDL    LKPEGWD L     +      + ++I+E HIRD S  DQ+V  
Sbjct: 413 LSTNSEYSQVVDLNDSALKPEGWDGLTMPHAQKTKADLAKMTIHESHIRDLSAWDQTVPA 472

Query: 368 DLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTF----------------------- 427
           +LRG Y+A T Q+S  + HLK+LS +G++H+ LLP F                       
Sbjct: 473 ELRGKYLALTAQESNMVQHLKQLSASGVTHIELLPVFDLATVNEFSDKVADIQQPFSRLC 532

Query: 428 ---------QFAGVDDDKKKWKSLDTKLLENLPPDSAEQQALIADIQNSDGYNWGYNPIL 487
                    +FAG  D     + + T+L +N   D+ + QAL   +  +D YNWGY+P  
Sbjct: 533 EVNSAVKSSEFAGYCDSGSTVEEVLTQLKQNDSKDNPQVQALNTLVAQTDSYNWGYDPFH 592

Query: 488 WGVPKGSYASDPNGPCRLIEFRKMVQALNQ-IGLRVVLDVVYNHLHGHGPFDPNSVLDKI 547
           + VP+GSYA+DP G  R+ EFR M+QA+ Q +G+ V++DVVYNH +  GP D  SVLDKI
Sbjct: 593 YTVPEGSYATDPEGTARIKEFRTMIQAIKQDLGMNVIMDVVYNHTNAAGPTDRTSVLDKI 652

Query: 548 VPGYYLRRN-TDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLM 607
           VP YY R N T G +E++TC +++A EH M  ++I D +  W  DYK+DGFRFDLMG+  
Sbjct: 653 VPWYYQRLNETTGSVESATCCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFRFDLMGYHP 712

Query: 608 KSTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVARNGRGVNASQFNLFGTGLGSF 667
           K+ +L A + +++L  +        IY +GEGWD  +   + R   ASQ NL GTG+G+F
Sbjct: 713 KAQILSAWERIKALNPD--------IYFFGEGWDSNQ---SDRFEIASQINLKGTGIGTF 772

Query: 668 NDRVRDAILGGSPF--GHPLQ--QGFVTGLLLEPNDHDHGTDEVAEFMLAVSKDHIQVAM 727
           +DR+RDA+ GG PF  G  L+  QG  +G  + PN+    +D+ A  +     D  ++ M
Sbjct: 773 SDRLRDAVRGGGPFDSGDALRQNQGVGSGAGVLPNELTTLSDDQARHL----ADLTRLGM 832

Query: 728 AANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPSETVNYASAHDNETLFDVVSLKTP 776
           A NL D+VL + +G   +G E+  ++G+P  YA+ P+E VNY S HDN+TL+D++S K  
Sbjct: 833 AGNLADFVLIDKDGAVKRGSEI-DYNGAPGGYAADPTEVVNYVSKHDNQTLWDMISYKAA 892

[Close]
BLAST of Spg009970 vs. ExPASy Swiss-Prot
Match: O33840 (Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 1.7e-63
Identity = 168/539 (31.17%), Postives = 272/539 (50.46%), Query Frame = 0 

Query: 210 VLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQAVQAQIYRDPVGGMPFEVIQLE-ELDGVW 269
           +LDD + Y+G LGAVYS E     +W+P ++ V+  ++++     P++V+ +E + +GVW
Sbjct: 215 ILDDYY-YDGELGAVYSPEKTIFRVWSPVSKWVKVLLFKNGEDTEPYQVVNMEYKGNGVW 274

Query: 270 RTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSDGRRTLFVDLGSDDLKPE 329
                    G +Y Y++  Y           T DPY++ V ++ +++  V+L   +  PE
Sbjct: 275 EAVVEGDLDGVFYLYQLENYGKIRT------TVDPYSKAVYANSKKSAVVNLARTN--PE 334

Query: 330 GWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGYMAFTLQDSAG----ITH 389
           GW+   D+ P +  + D  IYE+HI D +  + S   + +G Y+  T +++ G     T 
Sbjct: 335 GWEN--DRGPKIEGYEDAIIYEIHIADITGLENSGVKN-KGLYLGLTEENTKGPGGVTTG 394

Query: 390 LKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKLLENLPPDSAEQQALIADIQNSDG 449
           L  L   G++HVH+LP F F   D+  K ++                             
Sbjct: 395 LSHLVELGVTHVHILPFFDFYTGDELDKDFEKY--------------------------- 454

Query: 450 YNWGYNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLRVVLDVVYNHLHGHGPF 509
           YNWGY+P L+ VP+G Y++DP  P  R+ E ++MV+AL++ G+ V++D+V+ H +G G  
Sbjct: 455 YNWGYDPYLFMVPEGRYSTDPKNPHTRIREVKEMVKALHKHGIGVIMDMVFPHTYGIGEL 514

Query: 510 DPNSVLDKIVPGYYLR-RNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGF 569
              S  D+ VP Y+ R   T  ++  S C N  ASE  M+ + IVD + +WV +Y +DGF
Sbjct: 515 ---SAFDQTVPYYFYRIDKTGAYLNESGCGNVIASERPMMRKFIVDTVTYWVKEYHIDGF 574

Query: 570 RFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIYGEGW-DFGEVARNGRGVNASQF 629
           RFD MG + K TML+ + AL  +          +I +YGE W  +G   R G+       
Sbjct: 575 RFDQMGLIDKKTMLEVERALHKI--------DPTIILYGEPWGGWGAPIRFGKS------ 634

Query: 630 NLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLAVSKD 689
           ++ GT + +FND  RDAI  GS F +P  +GFV G         +G            + 
Sbjct: 635 DVAGTHVAAFNDEFRDAI-RGSVF-NPSVKGFVMG--------GYG-----------KET 658

Query: 690 HIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPSETVNYASAHDNETLFD 741
            I+  +  ++      N++GK +K            ++A  P ET+NYA+ HDN TL+D
Sbjct: 695 KIKRGVVGSI------NYDGKLIK------------SFALDPEETINYAACHDNHTLWD 658

[Close]
BLAST of Spg009970 vs. ExPASy Swiss-Prot
Match: C0SPA0 (Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.1e-59
Identity = 199/629 (31.64%), Postives = 289/629 (45.95%), Query Frame = 0 

Query: 160 PFTVPPESDVETLLKSQLAVATFNCKLSSLLCFSISYGQCNNITCLQLPGVL-----DDL 219
           P  V  E  +E   K  + V+        + C   S G   + T LQ+  V+     DD 
Sbjct: 44  PLAVREEYSLEAKYK-YVCVSDHPVTFGKIHCVRASSG---HKTDLQIGAVIRTAAFDDE 103

Query: 220 FSYEGPLGAVYSKEAVSLFLWAPTAQAVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPR 279
           F Y+G LGAVY+ +     +WAP A +   ++      G  F++ +LE+  GV+      
Sbjct: 104 FYYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEK--GVYAVTVTG 163

Query: 280 SWKGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLA 339
              G  YEY   + + S    E   T D YA+ V+ +G + +        L+P+     A
Sbjct: 164 DLHG--YEYLFCICNNS----EWMETVDQYAKAVTVNGEKGVV-------LRPDQMKWTA 223

Query: 340 DKKP---PVNSFSDISIYELHIRDFSISDQSVHPDLRGGYMAFTLQD-------SAGITH 399
             KP   PV    D  IYE H+RDFSI + S   + +G Y+A T  D       S+G+ +
Sbjct: 224 PLKPFSHPV----DAVIYETHLRDFSIHENSGMIN-KGKYLALTETDTQTANGSSSGLAY 283

Query: 400 LKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKLLENLPPDSAEQQALIADIQNSDG 459
           +K+L   G++HV LLP   FAGVD++K                               D 
Sbjct: 284 VKEL---GVTHVELLPVNDFAGVDEEKP-----------------------------LDA 343

Query: 460 YNWGYNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLRVVLDVVYNHLHGHGPF 519
           YNWGYNP+ +  P+GSYAS+P+ P  R  E ++M+  L+Q GLRV+LDVV+NH++     
Sbjct: 344 YNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKR--- 403

Query: 520 DPNSVLDKIVPGYYLRRNTDGFIENSTCV-NNTASEHFMVERMIVDDILHWVVDYKVDGF 579
             NS  +K VPGY+ R +  G   N T V N+ ASE  M  + I D +++W+ +Y VDGF
Sbjct: 404 -ENSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGF 463

Query: 580 RFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFG-EVARNGRGVNASQF 639
           RFDL+G L   T+L  K   +  TK K G     I ++GEGWD    +    +   A+  
Sbjct: 464 RFDLLGILDIDTVLYMK---EKATKAKPG-----ILLFGEGWDLATPLPHEQKAALANAP 523

Query: 640 NLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLAVSKD 699
            +   G+G FND  RDA+ G +   H    GF  G         +G    A         
Sbjct: 524 RM--PGIGFFNDMFRDAVKGNT--FHLKATGFALG---------NGESAQA--------- 560

Query: 700 HIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPSETVNYASAHDNETLFDV 759
                        V+    G    G + L    +P+     PS+++NY  +HDN T +D 
Sbjct: 584 -------------VMHGIAGS--SGWKAL----APIVPE--PSQSINYVESHDNHTFWDK 560

Query: 760 VSLKTPINITVNDRCRINHLATSIIALSQ 771
           +S   P       R R   LA +II L+Q
Sbjct: 644 MSFALPQENDSRKRSR-QRLAAAIILLAQ 560

[Close]
BLAST of Spg009970 vs. ExPASy TrEMBL
Match: A0A6J1G1P3 (pullulanase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449862 PE=4 SV=1)

HSP 1 Score: 1373.2 bits (3553), Expect = 0.0e+00
Identity = 675/774 (87.21%), Postives = 709/774 (91.60%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MS LFSSSLHLLP S P TSF + LPPRIAA SSSP  GG+FS VA TS S YP  H R 
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFS-VAATSLSHYPHGHSRC 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           GPLYCSSSASMSLEES  + SQLED+LLY+RAFWVSRYVIAWNVEVG+GSCYLF SKMAT
Sbjct: 61  GPLYCSSSASMSLEESTPA-SQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L V  GVVEGYD KIKLEKD GPL ENVIKKFPHIQNYCPFTVP  S VE LLK QLAVA
Sbjct: 121 LRVNDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TFN           SYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSL+LWAPTAQ
Sbjct: 181 TFN-----------SYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AVQAQIY++PVGG PFEVIQLEELDGVWRTKGP+SW+GCYYEYEVTVYHPSTL+VEKCF+
Sbjct: 241 AVQAQIYKEPVGGRPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFS 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RGVSSDGRRTLFVDLGSDDLKPEGWDKLAD+KPP++SFSDISIYELH+RDFSISD
Sbjct: 301 TDPYSRGVSSDGRRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPD+RGGYM FTLQDS GITHLKKLSNAG+SHVHLLPTFQF GVDDDK KWKSLD +
Sbjct: 361 QSVHPDMRGGYMGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLE LPPDSAEQQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA
Sbjct: 421 LLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIY+Y
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDHDHGTD+ AEF LAVSKDHIQVAMAANL+DYVL NFEGKEVKG EV T+DGS VAYA
Sbjct: 661 PNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCP+ETVNY SAHDNETLFDVVSLKTP NITVNDRCR+NHLATSIIALSQ   F
Sbjct: 721 SCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. ExPASy TrEMBL
Match: A0A5A7SZ60 (Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007110 PE=4 SV=1)

HSP 1 Score: 1357.0 bits (3511), Expect = 0.0e+00
Identity = 671/774 (86.69%), Postives = 700/774 (90.44%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MSLLFSSSLH  PS    TS RY L PR AA SSSPP  GLFSA AGTS S     +FR 
Sbjct: 1   MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSA-AGTSLSHGSHHNFRT 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           GPLYCS S+SMSLEES  STSQLED+LLYSRAFWVSR+VIAWNVEV +GSCYLFASKMAT
Sbjct: 61  GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L VK GVVEGYD KI LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121 LRVKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TF+           SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSL+LWAPTAQ
Sbjct: 181 TFD-----------SYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AV+AQI+RDPVGGMPFEVI LEE+DGVWRTKGP+SW+GCYYEYEVTVYHPSTLQVEKCFT
Sbjct: 241 AVRAQIFRDPVGGMPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFT 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RGVSSDGRRTLFVDL SDDL P+GWDKLAD KPPV+SF+DISIYELH+RDFSISD
Sbjct: 301 TDPYSRGVSSDGRRTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPDLRGGYMAFTLQDSAGI HLK LSNAGLSHVHLLPTF F GVDDDK KWK +DT+
Sbjct: 361 QSVHPDLRGGYMAFTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLENLPPDSAEQQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKMVQA
Sbjct: 421 LLENLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLH HGP D NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQ LTKEKNGVDGSSIYIY
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDH HGT EVAE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYA
Sbjct: 661 PNDHHHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCP+ETVNY SAHDNETLFD+VSLKT  NITV+DRCRINHLATSIIALSQ   F
Sbjct: 721 SCPTETVNYVSAHDNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. ExPASy TrEMBL
Match: A0A1S3BGM5 (pullulanase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489432 PE=4 SV=1)

HSP 1 Score: 1357.0 bits (3511), Expect = 0.0e+00
Identity = 671/774 (86.69%), Postives = 700/774 (90.44%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MSLLFSSSLH  PS    TS RY L PR AA SSSPP  GLFSA AGTS S     +FR 
Sbjct: 1   MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSA-AGTSLSHGSHHNFRT 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           GPLYCS S+SMSLEES  STSQLED+LLYSRAFWVSR+VIAWNVEV +GSCYLFASKMAT
Sbjct: 61  GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L VK GVVEGYD KI LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121 LRVKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TF+           SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSL+LWAPTAQ
Sbjct: 181 TFD-----------SYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AV+AQI+RDPVGGMPFEVI LEE+DGVWRTKGP+SW+GCYYEYEVTVYHPSTLQVEKCFT
Sbjct: 241 AVRAQIFRDPVGGMPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFT 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RGVSSDGRRTLFVDL SDDL P+GWDKLAD KPPV+SF+DISIYELH+RDFSISD
Sbjct: 301 TDPYSRGVSSDGRRTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPDLRGGYMAFTLQDSAGI HLK LSNAGLSHVHLLPTF F GVDDDK KWK +DT+
Sbjct: 361 QSVHPDLRGGYMAFTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLENLPPDSAEQQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKMVQA
Sbjct: 421 LLENLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLH HGP D NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQ LTKEKNGVDGSSIYIY
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDH HGT EVAE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYA
Sbjct: 661 PNDHHHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCP+ETVNY SAHDNETLFD+VSLKT  NITV+DRCRINHLATSIIALSQ   F
Sbjct: 721 SCPTETVNYVSAHDNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. ExPASy TrEMBL
Match: A0A0A0KTI2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622510 PE=4 SV=1)

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 669/774 (86.43%), Postives = 703/774 (90.83%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MSLLFSSSLH  P S   TS  Y L    AAFSSSPP        AGTS S     +FR 
Sbjct: 1   MSLLFSSSLHFFPPSAFPTSLCYQLHHPFAAFSSSPP-------AAGTSLSPGFRHNFRT 60

Query: 61  GPLYCSSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 120
           GPLYCS S+SMSLEES  STSQLED+LLYSRAFWVS++VIAWNVEV +GSCYLFASKMAT
Sbjct: 61  GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMAT 120

Query: 121 LCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVA 180
           L V+ GVVEGYD KI+LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121 LRVEDGVVEGYDVKIRLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 181 TFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQ 240
           TFN           SYG+C NITCLQLPGVLDDLFSYEGPLGA+YSKEAVSL+LWAPTAQ
Sbjct: 181 TFN-----------SYGECKNITCLQLPGVLDDLFSYEGPLGAIYSKEAVSLYLWAPTAQ 240

Query: 241 AVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFT 300
           AV+AQI+RDPVGGMPFEVI LEE+DG+WRTKGP+SWKGCYYEYEVTVYHPSTLQVEKCFT
Sbjct: 241 AVRAQIFRDPVGGMPFEVIPLEEVDGIWRTKGPKSWKGCYYEYEVTVYHPSTLQVEKCFT 300

Query: 301 TDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISD 360
           TDPY+RGVSSDGRRTLFVDL SDDL P+GWDKLAD+KPPV+SFSDISIYELH+RDFSISD
Sbjct: 301 TDPYSRGVSSDGRRTLFVDLFSDDLIPKGWDKLADEKPPVDSFSDISIYELHVRDFSISD 360

Query: 361 QSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTK 420
           QSVHPDLRGGYMAFTLQDSAGI HLKKLSNAGLSHVHLLPTFQF GVDDDK KWK +DT+
Sbjct: 361 QSVHPDLRGGYMAFTLQDSAGINHLKKLSNAGLSHVHLLPTFQFGGVDDDKTKWKFVDTE 420

Query: 421 LLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 480
           LLENLPPDSAEQQ+LIADIQNSDGYNWGYNPI+WGVPKGSYASD NGPCRL+EFRKMVQA
Sbjct: 421 LLENLPPDSAEQQSLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKMVQA 480

Query: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540
           LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF
Sbjct: 481 LNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHF 540

Query: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIY 600
           MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDAL+ LTKEKNGVDGSSIYIY
Sbjct: 541 MVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSIYIY 600

Query: 601 GEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660
           GEGWDFGEVA+NGRGVNASQFNLFGTG+GSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE
Sbjct: 601 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLE 660

Query: 661 PNDHDHGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYA 720
           PNDHDHGT EVAE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYA
Sbjct: 661 PNDHDHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYA 720

Query: 721 SCPSETVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           SCP+ETVNY SAHDNETLFD+VSLKTP NITV+DRCRINHLATSIIALSQ   F
Sbjct: 721 SCPTETVNYVSAHDNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPF 755

[Close]
BLAST of Spg009970 vs. ExPASy TrEMBL
Match: A0A5D3CD78 (Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002360 PE=4 SV=1)

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 670/801 (83.65%), Postives = 699/801 (87.27%), Query Frame = 0 

Query: 1   MSLLFSSSLHLLPSSTPVTSFRYILPPRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 60
           MSLLFSSSLH  PS    TS RY L PR AA SSSPP  GLFSA AGTS S     +FR 
Sbjct: 1   MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSA-AGTSLSHGSHHNFRT 60

Query: 61  GPLYCSSSASMSLEESAASTSQ------------LEDNLLYSRAFWVSRYVIAWNVEVGN 120
           GPLYCS S+SMSLEES  STSQ            LED+LLYSRAFWVSR+VIAWNVEV +
Sbjct: 61  GPLYCSPSSSMSLEES-TSTSQLAELVGYSVCQTLEDSLLYSRAFWVSRHVIAWNVEVES 120

Query: 121 GSCYLFASKMATLCVKGGVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESD 180
           GSCYLFASKMATL VK GVVEGYD KI LEKD   L ENVIKKFPHIQNYCPFTVPP SD
Sbjct: 121 GSCYLFASKMATLRVKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASD 180

Query: 181 VETLLKSQLAVATFNCKLSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKE 240
           VE LLK QLAVATF+           SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE
Sbjct: 181 VEALLKCQLAVATFD-----------SYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKE 240

Query: 241 AVSLFLWAPTAQAVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVY 300
            VSL+LWAPTAQAV+AQI+RDPVGGMPFEVI LEE+DGVWRTKGP+SW+GCYYEYEVTVY
Sbjct: 241 GVSLYLWAPTAQAVRAQIFRDPVGGMPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVY 300

Query: 301 HPSTLQVEKCFTTDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISI 360
           HPSTLQVEKCFTTDPY+RGVSSDGRRTLFVDL SDDL P+GWDKLAD KPPV+SF+DISI
Sbjct: 301 HPSTLQVEKCFTTDPYSRGVSSDGRRTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISI 360

Query: 361 YELHIRDFSISDQSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVD 420
           YELH+RDFSISDQSVHPDLRGGYMAFTLQDSAGI HLK LSNAGLSHVHLLPTF F GVD
Sbjct: 361 YELHVRDFSISDQSVHPDLRGGYMAFTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVD 420

Query: 421 DDKKKWKSL-------------------DTKLLENLPPDSAEQQALIADIQNSDGYNWGY 480
           DDK KWK +                   DT+LLENLPPDSAEQQ+LIADIQNSDGYNWGY
Sbjct: 421 DDKTKWKFVVSITCTWVMGPINMLSQMNDTELLENLPPDSAEQQSLIADIQNSDGYNWGY 480

Query: 481 NPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHLHGHGPFDPNSVL 540
           NPILWGVPKGSYASDPNGPCRL+EFRKMVQALNQIGLRVVLDVVYNHLH HGP D NSVL
Sbjct: 481 NPILWGVPKGSYASDPNGPCRLVEFRKMVQALNQIGLRVVLDVVYNHLHAHGPLDSNSVL 540

Query: 541 DKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGH 600
           DKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGH
Sbjct: 541 DKIVPGYYLRRNPDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGH 600

Query: 601 LMKSTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWDFGEVARNGRGVNASQFNLFGTGLG 660
           LMKSTMLKAKDALQ LTKEKNGVDGSSIYIYGEGWDFGEVA+NGRGVNASQFNLFGTG+G
Sbjct: 601 LMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAKNGRGVNASQFNLFGTGIG 660

Query: 661 SFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLAVSKDHIQVAMAA 720
           SFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDH HGT EVAE MLAVSKDHI+VAMAA
Sbjct: 661 SFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHHHGTHEVAESMLAVSKDHIEVAMAA 720

Query: 721 NLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPSETVNYASAHDNETLFDVVSLKTPIN 771
           NLRDY+LTNFEGKEVKGLEVLTHD SPVAYASCP+ETVNY SAHDNETLFD+VSLKT  N
Sbjct: 721 NLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAHDNETLFDIVSLKTSRN 780

[Close]
BLAST of Spg009970 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 1008.1 bits (2605), Expect = 3.9e-294
Identity = 476/709 (67.14%), Postives = 577/709 (81.38%), Query Frame = 0 

Query: 66  SSSASMSLEESAASTSQLEDNLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLCVKG 125
           S+S+ MSLE S+ + SQ  D L+YSRA+WV++ VIAWNV+VG GSCY +ASK A L    
Sbjct: 65  SASSPMSLEVSSPN-SQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSE 124

Query: 126 GVVEGYDAKIKLEKDCGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKSQLAVATFNCK 185
             ++GYD +IKLE + G L  +VI+KFPHI+NY  F VP + D+  L+KSQLAV  F+ +
Sbjct: 125 DGIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAE 184

Query: 186 LSSLLCFSISYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLFLWAPTAQAVQAQ 245
                      G+    T LQLPGVLD+LFSY+GPLGA ++ E VSL LWAPTAQAV   
Sbjct: 185 -----------GRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVC 244

Query: 246 IYRDPVGGMPFEVIQLEELDGVWRTKGPRSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYA 305
           IY++P+   P E+  L+E +GVW T+G  SW GCYY Y+V+VYHPST+++E C+  DPYA
Sbjct: 245 IYKNPLDKSPMEICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYA 304

Query: 306 RGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHP 365
           RG+S+DGR+T  V+L SDDLKPEGWD LADKKP + SFSDISIYELH+RDFS +D++V P
Sbjct: 305 RGLSADGRKTFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEP 364

Query: 366 DLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKLLENL 425
           + RGGY+AFT +DSAG+ HL+KL +AGL+H+HLLPTFQF  VDD+K+ WKS+DT LLE L
Sbjct: 365 ENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGL 424

Query: 426 PPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIG 485
            PDS E QA I +IQN DGYNWGYNP+LWGVPKGSYASDP GPCR+IEFRKMVQALN  G
Sbjct: 425 RPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTG 484

Query: 486 LRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERM 545
           L VVLDVVYNHLH  GP D  SVLDKIVPGYYLRRN+DGFIENSTCVNNTASEH+MV+R+
Sbjct: 485 LNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRL 544

Query: 546 IVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYIYGEGWD 605
           I DD+L+WVV+YKVDGFRFDLMGH+MK+T++ AK A+ SL KE +GVDGS IY+YGEGW+
Sbjct: 545 IRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWN 604

Query: 606 FGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHD 665
           FGEVA NGRG+NASQFNL GTG+GSFNDR+RDA LGGSPFGHPLQQGF+TGLLL+PN HD
Sbjct: 605 FGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHD 664

Query: 666 HGTDEVAEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPSE 725
           HG++   E ML+ +K+HIQ  MAANL+DY+LTN EGKEVKG EVL HD +PVAYAS P+E
Sbjct: 665 HGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTE 724

Query: 726 TVNYASAHDNETLFDVVSLKTPINITVNDRCRINHLATSIIALSQVFNF 775
           T+NY SAHDNETLFD++SLKTP+ I+V++RCRINHLA+S+IALSQ   F
Sbjct: 725 TINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPF 761

[Close]
BLAST of Spg009970 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 122.1 bits (305), Expect = 2.0e-27
Identity = 99/354 (27.97%), Postives = 158/354 (44.63%), Query Frame = 0 

Query: 302 DPYARGVS-----SDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDF 361
           DPYA+ V       D  +      G+ D +   +D   D K P     D+ IYE+++R F
Sbjct: 187 DPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAF 246

Query: 362 SISDQS-VHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWK 421
           +  + S + P + G Y+ F       I  +  L + G++ V LLP F+F  ++  ++   
Sbjct: 247 TADESSGMDPAIGGSYLGF-------IEKIPHLQDLGINAVELLPVFEFDELELQRR--- 306

Query: 422 SLDTKLLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLI-EF 481
                   + P D                  WGY+ + +  P   YAS    P +   EF
Sbjct: 307 --------SNPRDHMVN-------------TWGYSTVNFFAPMSRYASGEGDPIKASKEF 366

Query: 482 RKMVQALNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPG--YYLRRNTDGFIENSTCV 541
           ++MV+AL+  G+ V+LDVVYNH +      P +   + +    YY+    +  +  S C 
Sbjct: 367 KEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCG 426

Query: 542 NNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKST---MLKAKDALQSLTKEK 601
           N     H +V  +I+D + HWV +Y VDGFRFDL   L ++T    L A   ++++ K+ 
Sbjct: 427 NTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDS 486

Query: 602 NGVDGSSIYIYGEGWDFGEVARNGRGVNASQFNLFGTGLGSFNDRVRDAILGGS 644
                S   I  E WD G +   G+  N   ++ +    G + D VR  I G S
Sbjct: 487 ---VLSRCKIIAEPWDCGGLYLVGKFPN---WDRWAEWNGMYRDDVRRFIKGDS 503

[Close]
BLAST of Spg009970 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-15
Identity = 82/294 (27.89%), Postives = 131/294 (44.56%), Query Frame = 0 

Query: 302 DPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNS-------------FSDISI 361
           DPYA+ + S   R  F  LG DD     W ++A   P                   D+ I
Sbjct: 167 DPYAKAIIS---RDEFGVLGPDD---NCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVI 226

Query: 362 YELHIRDFSISDQSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVD 421
           YE+H+R F+   +S   +  G Y       +  + HLK+L   G++ + L+P  +F    
Sbjct: 227 YEMHVRGFT-RHESSKIEFPGTYQGV----AEKLDHLKEL---GINCIELMPCHEF---- 286

Query: 422 DDKKKWKSLDTKLLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSY--ASDPN 481
            ++ ++ S +T               ++ D + +    WGY+ I +  P   Y  AS  N
Sbjct: 287 -NELEYYSYNT---------------ILGDHRVN---FWGYSTIGFFSPMIRYASASSNN 346

Query: 482 GPCRLI-EFRKMVQALNQIGLRVVLDVVYNHL---HGHGPFDPNSVLDKIVPGYYLRRNT 541
              R I EF+ +V+  ++ G+ V++DVV NH    +  GP      +D  V  YY+    
Sbjct: 347 FAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSV--YYMLAPK 406

Query: 542 DGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTML 577
             F   S C N     H +V + I+D + +WV +  VDGFRFDL   + +S+ L
Sbjct: 407 GEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSL 421

The following BLAST results are available for this feature:
BLAST of Spg009970 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038892369.10.0e+0087.86pullulanase 1, chloroplastic [Benincasa hispida] >XP_038892370.1 pullulanase 1, ... [more]
XP_023542048.10.0e+0087.34pullulanase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022945721.10.0e+0087.21pullulanase 1, chloroplastic [Cucurbita moschata][more]
KAG6573853.10.0e+0087.08Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_008446816.10.0e+0086.69PREDICTED: pullulanase 1, chloroplastic [Cucumis melo] >KAA0034691.1 pullulanase... [more]
BLAST of Spg009970 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8GTR45.6e-29367.14Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2[more]
P072061.0e-11634.04Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2[more]
P078115.5e-11534.09Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1[more]
O338401.7e-6331.17Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / N... [more]
C0SPA01.1e-5931.64Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1[more]
BLAST of Spg009970 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G1P30.0e+0087.21pullulanase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449862 PE=4 ... [more]
A0A5A7SZ600.0e+0086.69Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007110 ... [more]
A0A1S3BGM50.0e+0086.69pullulanase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489432 PE=4 SV=1[more]
A0A0A0KTI20.0e+0086.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622510 PE=4 SV=1[more]
A0A5D3CD780.0e+0083.65Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002360... [more]
BLAST of Spg009970 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G04360.13.9e-29467.14limit dextrinase [more]
AT4G09020.12.0e-2727.97isoamylase 3 [more]
AT2G39930.11.3e-1527.89isoamylase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040671Pullulanase, N2 domainPFAMPF17967Pullulanase_N2coord: 90..213
e-value: 4.2E-20
score: 72.7
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 320..775
e-value: 1.9E-188
score: 629.4
NoneNo IPR availableGENE3D2.60.40.1130coord: 88..213
e-value: 1.5E-25
score: 92.4
NoneNo IPR availableCDDcd11341AmyAc_Pullulanase_LD-likecoord: 345..770
e-value: 8.09652E-151
score: 444.644
NoneNo IPR availableCDDcd02860E_set_Pullulanasecoord: 219..320
e-value: 5.48755E-33
score: 120.34
IPR004193Glycoside hydrolase, family 13, N-terminalPFAMPF02922CBM_48coord: 220..305
e-value: 2.1E-10
score: 40.8
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 214..319
e-value: 1.5E-26
score: 94.6
IPR011839Alpha-1,6-glucosidases, pullulanase-typeTIGRFAMTIGR02103TIGR02103coord: 87..774
e-value: 5.5E-262
score: 869.7
IPR045041Pullulanase PULA-likePANTHERPTHR43631PULLULANASE 1, CHLOROPLASTICcoord: 67..774
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 213..322
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 79..214
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 318..770

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009970.1Spg009970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019252 starch biosynthetic process
biological_process GO:0005983 starch catabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0010303 limit dextrinase activity
molecular_function GO:0051060 pullulanase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds