Spg009846 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009846
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionglutathione S-transferase DHAR2-like
Locationscaffold7: 8287498 .. 8294060 (+)
RNA-Seq ExpressionSpg009846
SyntenySpg009846
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGATATACCCACCCTGGGTGTCGTTAAACTTAAAAGAAATGAAAACCGAGGGAAACGAGCTTCAAACGAGCTCCAGTAATCTCCCTTTACTACTTTTTTGTCAACTCATAAGTGATAAGCTCTTTGAACGTTGGCAATTTTTCGGATCATATTCGATACCCCATATCCAAGCTTTTCTTTTTCTATACTTTTGATCGATCGATGCCAGAAATGCTTTGAAATCTCTCGAGATACCTGATTCCTGCCTGAATGTTCTGGACCTTCTTGTCCTCGTCCTATAATTTCACCGACCCGCCATGGGAATCGAGAGCACAGAACTTCAGTTTATACCCAAATTCCCACAATCACTCGCCATTCGCATCCTCTGTTTCTTCTGTAATTTCCAGTTTCAAAGTTCCCCACCTGCCCCTTCGATTGCAAAATGGCCATTGAGGTTGCTGCCAAGGCTGCCGCTGGTGCACCCGATGTTATGGGAGACTGTATGTTCCTTAGCCAAATTTTGTATTGAGTTTTTTGTTATATTATTATTATTATTATTATTATTATTTTCTCTTGCAGGCCCTTTCAGCCAGAGGGTTCTCTTAACTTTGGAGGAGAAGGAAGTTCCTTACGAATTGCATCTCATCGATTTATCCGACAAACCCAGTTGGTAATATCTCGAAACTTGGCTTTAGTTTCAGTTCATTTGGGTTGCAAGAGAAAGAGGGGTTTTAAGCAAATGGGTCGTGTGGATTGTTAATGATTTTTGCAGGTTCTTGGAAGTGAGCCCAGAAGGAAAGGTGCCGGTGGCGAAGATTGATGACAAATGGGTTCCTGACTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTTGTTACCCCTCCTGAATTTTCTTCTGTGTAAGGCCTTTTCCTCCTTTTTTTTTGGTTTTATTTTTTTTAGTTGAAGAATGTATAGAATTGGTGATTTGAATCTCTGACATTGTGGTAGATGATTTTTACCTTAACCAGTTAAGCCATGTTCAAGTTGGCTTTTCTGGTTTGAGTATGTTTTAAGGATGAAGCTTATGCCTCATTTGCTTTCTATTCACTTTTTGTTGAGTAATGTTTTCAACAGTTCATATTTGAAGTACCACATGCCTGTGTGTTTGTTTGATGAGTCCATTTCCATGTCTCATCTGACTTCTGAATCTCTGATTGTCCCTTATTTTAGTAGCATGTTTGGTAATGTTGTAGCTAAGGTCTCGTTTGATAACTATTTGATTTTTGGTCCTTGGTGTTTGGAAATTAAGCTTAGAAACACTACTTTCACCCATACATTTCTTTGGTTTGGGTTTGTTATTTATCTTTTTCCTATGTTTTCAAAAACTAAGTTAGTTTTGAAAACTAAAAAAGTAGTTTTCAAAAACTTGTTTCTGTTCTTGGAATTGATTAGAAATTTAAATGTGTGCTTAAGAAAGATGAAAACCATTGTACGAGAATTGGGAGAAAGCAAACATAACTTCCAAAAACCAAAAACAAAAAATCAAATGGTTATCAAATGAGACCTAAATTTTTCGTGATTTGGAATCAATGTTTCTTTTCCTCTTATGGAACCAGGCTGCTGCCTTTTGTTCTTTTTGTATTTTTTGGGATAAGAACACTATAACTTCATTGCTAGAAACAATACGTACAAAACTTTTTTCTAGTTGGAAGCTATCAAGGAGAAGTGCCCCAAATCATCAATTTCCGAAGTTTTTCCATAGCCTCTACTTGAAAGATTCAGTTGTTTCTTTTACCTGGCCCATAACAATTATGATTGTATTACACTAAGGCGTTAGGTGTAGGTAGGTTCAAGGTCTGATCATAATCCAGATGAAGACAGCTTAATTTTTAACTGGTTTTTTGAAGAATTAAAATCATGTTACTTTTTCTGAATTTTACGAATTTCTTGTTTCACTACATTTTAATCCGCAAAGGAAGTGTCCGAATTTGGTCTGCAATTGGTGTGTCCTGGAACAATCATTTACTACAGAATAAAGTCGTTGAGATTGTACTAATAATGAAGGTCAAAATAAACTCCTGATTTTTGAAATTTTAGTTGTCTTAATGGTATTGCTATTGCCTAATTCAGTACTTGGACATGGCATTGGCCGAGTTTGTAATTGTGATGCCTTCCCAAATTAAAACATCGACTATGCTTTGGTTTTGACTTCTGATACAATATCCTCTGTTTACTTTGTCTCGTAGGGGATCAAAGATAAATGGGGCTTTTGTAAAATTCTTGAAGAGCGAGGACCCAAATGATGGTTCAGAGCAGGCTTTGCTTGAAGAGTTGAATGCGTTAGACGAGCATCTTAAGGCACACGTATGTGTGTGTAATATATCAAACAACTATTTTGAAGTGTTTATGATTTTACTCTTCTCATTTTTGGCAATGCCATGTGGTGCCAGGGTCCTTATGTTGCTGGAGAGAAGGTTACTGCTGTTGATTTGAGTCTGGCACCAAAGTTGTACAGTCTTGATATTGCTCTTGGCCATTTCAAAGAGTGGACTTTCCCTGAAAACTTAACCAGTTTATCCGCATACAAAGAGGTACGATGTAGTTGCACCAACGGTACATAGTTTGTGATTTATAATTAAATTAGATCTTTTTATTTTTTTTGATGAACAAATTAGATCTTTTTAAGCCACCATCTGGTTTATATTTGTTATTAATTTCTTTTTTTTGTTAAATTACAAGTTTAGTTTCTGAACTTTCAAGCTTGTATCTATTTGGTTCCTTATCTCTAAACTTTAAAAAGGGTCTAATTAGGTCCTTGAACTTTCAATTTTGTATCTAATAAGTCTCTGAATTTTCAATTTTGTGTCCAATATGTCCTCGAATTTTCCATTTTGTGTCTCATTGGTCATTGACCTATTCCACATTTTTTTAGAATTCATGAATCTATTATACACAAAATAGAAAGTTTAGAGTCCACTTAGATATAAAATTCAATTTTATATCTAATGGATCAGTTAACTTTTAAAAATTTTGAACATGTCAAGGACATGATAGACACAAAATTGGAAGTTTGGGACTTATTACACATTTTTAAAGTTCAAGGACCTAGTGGATGAACAATTGGAAGTTCAGGGGCTTATTACTCACTTTTCAAAGTCCAGAGACTTTTTAGACACAAACTTAAAAGTTTTGGGACTAAATTTGTGATTTAACCAAAATTTGCTCTCTCTCTCTCTCCTTAGACTTTGCTGGCTTCTTAGATTACCTGCAGTTTGAAATCTACTGTGCTGGTTGCTTTCAAGCTCAACTAAACTTGAACCGACAGTAATTAGGTGTGTGTATAACTTATAGAAAATCCTGGGATAAACTTTGTTCTTCTTTTAAGGGTAGACGACAGGTTCAATATAACCACTCCCACTAAACTTAGTTTGTTATGCACAAGTCAAATATAATCCCGATGAGTGGGGTGCAAGTTCATCTAGTTTCTTGGAGCTGGAAATGATCGAGATTGAAAATTGTTTAATCATAATGTAGGGTGTTTTAGCCAGAGCAAGAAAAGTGAAAGAATAGAAGGGGCGGTAGGGGGGCAGCTTGGGGTGGAAAACCATCCAATGGATGCATTACATGATATGCTCTGATCAATTGCCATTGGTTGGATTGTTGATTTTGCAGTTGCTTTTTGCTCGAGAATCTTTCATGAAGACGAAGTCTGCCCCCGAACATGTAATTTCTGGTTGGGAGCCCAAGGTGAATGCTGCAAAAACCAGGATATATTTTCATCACACGTTATGAAATGTTGGGGGTTTATAATCCTAACCATGGGACTTTGGTTGCCATTTTCCTTTGCCTCAATAAAATGGTGTTTTATCACATCCAAGTTTCTTCTCTAATTCCCAGATTCAAACACTCCCCCACTGCCCACATGATCTGGTCCTTGAAGCTGCGGTCAAGGCTGCCGTTGGTGCCCCTGATGTACTTGGGGACTGTATGTTCTTTCCCTTTCATCTTTAGGAATTTTTTCGAGTTCTTGTCAATTTTATGATTGGGGTTTGTTTTTTCTTGCAGGCCCTTTCAGCCATAGGGTTCTCTTAACTTTAGAGGAGAAGAAAGTTGCTTACAAAATGCATCTCATTGATCTCTCAGACAAACCCAGCTGGTAATATCTCGAAATTTGGGTTGCGAGAAATAAGAGGGTTTTAAGCAAATGGGGTTGTGTGGATTCATGATGATTTTGGCAGGTTCTTGGAAGTGAGCCCTGAAGGGAAGGTGCCGGTTGTGAAGTTTGATGATAAATGGGTTCCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCGCTTGTTACTCCTCCTGAAGTTTCTTCTGTGTAAGTTTTTTTTCTTTAACCCTTTTCTGATATGATCCTTTGTTTCATATCTGAGTGAAGTTTATGCCTTCTGTTTGCTGCGCATTTATGTTGACTAATGTTTTCAACATTCTTATTTGAAGTTTTTATTTGTTGAGTTGGGATGATTGTAACCACACGTCTTTGTGTGTGTTTGATGTGTCCATTTCTTTTCCATGTCTCACTGTTTTCTAGTTTGACCTTTACTTTAGCCGAATGTTTGGTCCCGTCCTATTGTAGCTAAATTTGTCTTGTGATTTGTGAAACCCATGTTTTTTTCCTCGTATGGAGTCACTGTCAGGTTGATGGTTCTTTCTCTCTCTTTTTTTGGGATAAGATGACTAGAATCTCATTGATAGAAACAATATGTACAGAGGATTCAAAGGATTTAAAATGAATATATGAAAAACAAAACAAACTCTGAAGTGTTTCCATATCCCCACTAGTCTCATCTTGAAAGATCCAGTTGTTTCTTTCATCCAGTTGATCCAAGACAATAGTGTCTGTTTTTCAGAGGCAGCTGAATTTTTTTCTTTTGGAGGGGCATGATTGTATTACATTAAGGCATTAGTTAGGTTCAAGATCTAGATAACCATTATACAGATGAAGAAAACGTATGGGCCAAAAACACTTCCCACCTCAATGGTTTGGAGTAATAGAAAAGGTTCAAGTACTTCAAAGTGTTTTGTGAGAAAGTATTTGTAGAGTGTTTGTTGGCTTAAGTACTTGACTGGAAAGCACTAAAAGGTACTTTTATTAAAATGTAGAGCTAAACATCAGAATGATTTGTTATTATACCCTTCTCATAAAAATTACTTATAATTTAATTACTATTGTTAAAAGCAGTTATTCTGAAATCATTTCCAAATCAGTATTCTTACCTAATTCAGTACTCCCATTATGGCATCAGCAAGATTGTAATTGTGAAGGTTTTCTTCAACTAAATCACAAACTATGCTTTGGTTCTGACTCTGAAAAAAGTGTCCTCTCTTTACTCTGGCTAATAGGGGATCAAAGATATTTGGTGCTTTTGTAACATTCTTGGAGAGCAAGGACCCAGATGATGGTTCAGAGCAGGCTTTCCTTGAAGAGTTGAAGGCGTTCAATGAGCATCTTAAGGCTCATGTATGTATGTGTAGTTCATAGAACAACCATATTTAAGTGTTTATGATTTTACTCTTCTCATATTTAGCAATGCCATGTGGTGCCAGGGTCCTTACGTCGCTGGAGAGAAGGTAACTGCTGTTGATTTGAGTTTGGCACCAAAGTTGTACCATGTTGATGTTGCTCTTGGCCATTTCAAGAAGTGGGCTATCCCCAAAGACTTGGCCAGTTTAATCGCCTACAAAGAGGTACATTGTAGTTGCACGAACGCTGGCTCATGACTTTTAATTCAATTAAATCTTTGTGAGCCAGTATCAGATTGGTTACTAGTTTTGTTTGCTTACTCTTATGTTTAGACTTTGCTAGCTTCTGAGATTGCCTGCAGTTTGAAATCTATTATGCTGGTTGGTTTCTGCTCAACCAAACTCGAATTTGCAGTAATGGGGTGTGTGTCTAACTGATGGAGAATCATAGCTTTGTTCTCTAAGTGTAGTAAGTATAGACAGGTCAACTTAAGCCACTCCCACTTAATTTAGTTTGTTATGCACAAATCAAATATAACCCCAATGAGTGGGATGTGAATTCATCTAGTTCAGGATTGATATTTGTTCAATCCCCGGGTTGGGGAGTCTGATGAACTAGCTTGGGGTACCATAGATGCATTAGATGTTATGCTCTGATGAATTGTTGGTGGTGGCATTGTTCATTTTGCAGTTGCTTTTCGCTCGAGAATCTTTCGTGAAGACGAAGGCTGCCCCGGAACATGTAATTGCTGGATGGGAGCCCAAGGTGAATCCTGCATGAATCAGGAGATGTTTTCATCCCAAGTTATGAAATAGTTGGGGGTTTAATCCTGAGAATGAGATTGTGGTTGCTAGGTTGCTTGCTGTTTTCCTATGTTTCAATAAACAGTGTACTTTCATATGTGCCTCAATAGAAGAGTGTGTTGTCACTATGCCTCATCTTTTAAGAATGCCTATGTTTAGGAAAAAGCTTTGTTTCTTCTTCTTCCCTGTTTCCTTCTCCTACCCCACCATTTTTGGAGAGCTTTGGTTATATGAAGGTAAAACAAACAAGAG

mRNA sequence

ATGGCCATTGAGGTTGCTGCCAAGGCTGCCGCTGGTGCACCCGATGTTATGGGAGACTGCCCTTTCAGCCAGAGGGTTCTCTTAACTTTGGAGGAGAAGGAAGTTCCTTACGAATTGCATCTCATCGATTTATCCGACAAACCCAGTTGGTTCTTGGAAGTGAGCCCAGAAGGAAAGGTGCCGGTGGCGAAGATTGATGACAAATGGGTTCCTGACTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTTGTTACCCCTCCTGAATTTTCTTCTGTGGGATCAAAGATAAATGGGGCTTTTGTAAAATTCTTGAAGAGCGAGGACCCAAATGATGGTTCAGAGCAGGCTTTGCTTGAAGAGTTGAATGCGTTAGACGAGCATCTTAAGGCACACGGTCCTTATGTTGCTGGAGAGAAGGTTACTGCTGTTGATTTGAGTCTGGCACCAAAGTTGTACAGTCTTGATATTGCTCTTGGCCATTTCAAAGAGTGGACTTTCCCTGAAAACTTAACCAGTTTATCCGCATACAAAGAGTTGCTTTTTGCTCGAGAATCTTTCATGAAGACGAAGTCTGCCCCCGAACATGTAATTTCTGGTTGGGAGCCCAAGATTCAAACACTCCCCCACTGCCCACATGATCTGGTCCTTGAAGCTGCGGTCAAGGCTGCCGTTGGTGCCCCTGATGTACTTGGGGACTGCCCTTTCAGCCATAGGGTTCTCTTAACTTTAGAGGAGAAGAAAGTTGCTTACAAAATGCATCTCATTGATCTCTCAGACAAACCCAGCTGGTTCTTGGAAGTGAGCCCTGAAGGGAAGGTGCCGGTTGTGAAGTTTGATGATAAATGGGTTCCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCGCTTGTTACTCCTCCTGAAGTTTCTTCTGTGGGATCAAAGATATTTGGTGCTTTTGTAACATTCTTGGAGAGCAAGGACCCAGATGATGGTTCAGAGCAGGCTTTCCTTGAAGAGTTGAAGGCGTTCAATGAGCATCTTAAGGCTCATGGTCCTTACGTCGCTGGAGAGAAGGTAACTGCTGTTGATTTGAGTTTGGCACCAAAGTTGTACCATGTTGATGTTGCTCTTGGCCATTTCAAGAAGTGGGCTATCCCCAAAGACTTGGCCAGTTTAATCGCCTACAAAGAGACTTTGCTAGCTTCTGAGATTGCCTGCAGTTTGAAATCTATTATGCTGGTTGGTTTCTGCTCAACCAAACTCGAATTTGCATTGCTTTTCGCTCGAGAATCTTTCGTGAAGACGAAGGCTGCCCCGGAACATGTAATTGCTGGATGGGAGCCCAAGGTGAATCCTGCATGA

Coding sequence (CDS)

ATGGCCATTGAGGTTGCTGCCAAGGCTGCCGCTGGTGCACCCGATGTTATGGGAGACTGCCCTTTCAGCCAGAGGGTTCTCTTAACTTTGGAGGAGAAGGAAGTTCCTTACGAATTGCATCTCATCGATTTATCCGACAAACCCAGTTGGTTCTTGGAAGTGAGCCCAGAAGGAAAGGTGCCGGTGGCGAAGATTGATGACAAATGGGTTCCTGACTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCTCTTGTTACCCCTCCTGAATTTTCTTCTGTGGGATCAAAGATAAATGGGGCTTTTGTAAAATTCTTGAAGAGCGAGGACCCAAATGATGGTTCAGAGCAGGCTTTGCTTGAAGAGTTGAATGCGTTAGACGAGCATCTTAAGGCACACGGTCCTTATGTTGCTGGAGAGAAGGTTACTGCTGTTGATTTGAGTCTGGCACCAAAGTTGTACAGTCTTGATATTGCTCTTGGCCATTTCAAAGAGTGGACTTTCCCTGAAAACTTAACCAGTTTATCCGCATACAAAGAGTTGCTTTTTGCTCGAGAATCTTTCATGAAGACGAAGTCTGCCCCCGAACATGTAATTTCTGGTTGGGAGCCCAAGATTCAAACACTCCCCCACTGCCCACATGATCTGGTCCTTGAAGCTGCGGTCAAGGCTGCCGTTGGTGCCCCTGATGTACTTGGGGACTGCCCTTTCAGCCATAGGGTTCTCTTAACTTTAGAGGAGAAGAAAGTTGCTTACAAAATGCATCTCATTGATCTCTCAGACAAACCCAGCTGGTTCTTGGAAGTGAGCCCTGAAGGGAAGGTGCCGGTTGTGAAGTTTGATGATAAATGGGTTCCTGATTCTGATGTCATTGTGGGGATTCTTGAGGAGAAGTATCCAGAGCCTTCGCTTGTTACTCCTCCTGAAGTTTCTTCTGTGGGATCAAAGATATTTGGTGCTTTTGTAACATTCTTGGAGAGCAAGGACCCAGATGATGGTTCAGAGCAGGCTTTCCTTGAAGAGTTGAAGGCGTTCAATGAGCATCTTAAGGCTCATGGTCCTTACGTCGCTGGAGAGAAGGTAACTGCTGTTGATTTGAGTTTGGCACCAAAGTTGTACCATGTTGATGTTGCTCTTGGCCATTTCAAGAAGTGGGCTATCCCCAAAGACTTGGCCAGTTTAATCGCCTACAAAGAGACTTTGCTAGCTTCTGAGATTGCCTGCAGTTTGAAATCTATTATGCTGGTTGGTTTCTGCTCAACCAAACTCGAATTTGCATTGCTTTTCGCTCGAGAATCTTTCGTGAAGACGAAGGCTGCCCCGGAACATGTAATTGCTGGATGGGAGCCCAAGGTGAATCCTGCATGA

Protein sequence

MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSEQALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLSAYKELLFARESFMKTKSAPEHVISGWEPKIQTLPHCPHDLVLEAAVKAAVGAPDVLGDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQAFLEELKAFNEHLKAHGPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAYKETLLASEIACSLKSIMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA
Homology
BLAST of Spg009846 vs. NCBI nr
Match: XP_022985954.1 (uncharacterized protein LOC111483829 isoform X3 [Cucurbita maxima])

HSP 1 Score: 722.2 bits (1863), Expect = 2.7e-204
Identity = 367/463 (79.27%), Postives = 387/463 (83.59%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAIGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKIQT----LPHCPHDLVLEAAVKAAVGAPDVL 240
            YKELLFARESFMKTK+APEHVISGWEPK+      +        +E AVKAAVGA DV+
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAVPVISDSEPKAIEVAVKAAVGALDVI 240

Query: 241 GDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIV 300
           GDCPF+ RVLLTLEEKKV YK HLIDLSDKP WFLEVSPEGKVPV K DD+WVPDSDVIV
Sbjct: 241 GDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPCWFLEVSPEGKVPVAKIDDRWVPDSDVIV 300

Query: 301 GILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQAFLEELKAFNEHLKAH 360
           GILEEKYPEP L TPPE SSVGSKI GAFV FL+SKDP DGSEQA LEELKA NEHLKAH
Sbjct: 301 GILEEKYPEPFLATPPEFSSVGSKINGAFVKFLKSKDPSDGSEQALLEELKALNEHLKAH 360

Query: 361 GPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAYKETLLASEIACSLKS 420
           GPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  YKE             
Sbjct: 361 GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKE------------- 420

Query: 421 IMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
                         LLFARESFVKTKAAPEHVI+GWE KVN A
Sbjct: 421 --------------LLFARESFVKTKAAPEHVISGWEAKVNDA 436

BLAST of Spg009846 vs. NCBI nr
Match: XP_023547271.1 (uncharacterized protein LOC111806131 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 721.8 bits (1862), Expect = 3.6e-204
Identity = 367/463 (79.27%), Postives = 389/463 (84.02%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W  P NLTSLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWISPVNLTSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKI----QTLPHCPHDLVLEAAVKAAVGAPDVL 240
            YKELLFARESFMKTK+APEHVISGWEPK+    + +        +E AVKAAVGA DV+
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNATAEPVISDSEPKAIEVAVKAAVGALDVI 240

Query: 241 GDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIV 300
           GDCPF+ RVLLTLEEKKV YK HLIDLSDKPSWFLEVSPEGKVPV K DDKWVPDSDVIV
Sbjct: 241 GDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIV 300

Query: 301 GILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQAFLEELKAFNEHLKAH 360
           GILEEKYPEP L TPPE S+VGSKI GAFV FL+SKDP+DGSEQA LEELKA NEHLKAH
Sbjct: 301 GILEEKYPEPFLATPPEFSTVGSKINGAFVKFLKSKDPNDGSEQALLEELKALNEHLKAH 360

Query: 361 GPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAYKETLLASEIACSLKS 420
           GPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  YKE             
Sbjct: 361 GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKE------------- 420

Query: 421 IMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
                         LLFARESFVKTKAAPEHVI+GWE KV  A
Sbjct: 421 --------------LLFARESFVKTKAAPEHVISGWEAKVKAA 436

BLAST of Spg009846 vs. NCBI nr
Match: XP_022943191.1 (uncharacterized protein LOC111447994 isoform X3 [Cucurbita moschata])

HSP 1 Score: 721.8 bits (1862), Expect = 3.6e-204
Identity = 367/463 (79.27%), Postives = 388/463 (83.80%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWV DSDVIVGILEEKYPEPSLVTPPEFSSVGSK NGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVTPPEFSSVGSKTNGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKI----QTLPHCPHDLVLEAAVKAAVGAPDVL 240
            YKELLFARESFMKTK+APEHVISGWEPK+    + +        +E AVKAAVGA DV+
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAEPVISDSEPKAIEVAVKAAVGALDVI 240

Query: 241 GDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIV 300
           GDCPF+ RVLLTLEEKKV YK HLIDLSDKPSWFLEVSPEGKVPV K DDKWVPDSDVIV
Sbjct: 241 GDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIV 300

Query: 301 GILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQAFLEELKAFNEHLKAH 360
           GILEEKYPEP L TPPE SSVGSKI GAFV FL+SKDP+DGSEQA LEELKA NEHLKAH
Sbjct: 301 GILEEKYPEPFLATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLKAH 360

Query: 361 GPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAYKETLLASEIACSLKS 420
           GPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  YKE             
Sbjct: 361 GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKE------------- 420

Query: 421 IMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
                         LLFARESFVKTKAAPEHVI+GWE KVN A
Sbjct: 421 --------------LLFARESFVKTKAAPEHVISGWEAKVNAA 436

BLAST of Spg009846 vs. NCBI nr
Match: XP_022985944.1 (probable glutathione S-transferase DHAR2, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 717.2 bits (1850), Expect = 8.8e-203
Identity = 367/478 (76.78%), Postives = 388/478 (81.17%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAIGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKI-------------------QTLPHCPHDLV 240
            YKELLFARESFMKTK+APEHVISGWEPK+                   + +        
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAVPVISDSEPKVNAATEPVVSDSEPKA 240

Query: 241 LEAAVKAAVGAPDVLGDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPV 300
           +E AVKAAVGA DV+GDCPF+ RVLLTLEEKKV YK HLIDLSDKP WFLEVSPEGKVPV
Sbjct: 241 IEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPCWFLEVSPEGKVPV 300

Query: 301 VKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQA 360
            K DD+WVPDSDVIVGILEEKYPEP L TPPE SSVGSKI GAFV FL+SKDP DGSEQA
Sbjct: 301 AKIDDRWVPDSDVIVGILEEKYPEPFLATPPEFSSVGSKINGAFVKFLKSKDPSDGSEQA 360

Query: 361 FLEELKAFNEHLKAHGPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAY 420
            LEELKA NEHLKAHGPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  Y
Sbjct: 361 LLEELKALNEHLKAHGPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKY 420

Query: 421 KETLLASEIACSLKSIMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
           KE                           LLFARESFVKTKAAPEHVI+GWE KVN A
Sbjct: 421 KE---------------------------LLFARESFVKTKAAPEHVISGWEAKVNDA 451

BLAST of Spg009846 vs. NCBI nr
Match: XP_022943190.1 (probable glutathione S-transferase DHAR2, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 716.1 bits (1847), Expect = 2.0e-202
Identity = 367/478 (76.78%), Postives = 388/478 (81.17%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWV DSDVIVGILEEKYPEPSLVTPPEFSSVGSK NGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVTPPEFSSVGSKTNGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKI-------------------QTLPHCPHDLV 240
            YKELLFARESFMKTK+APEHVISGWEPK+                   + +        
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAEPVISDSEPKVNAAAEPVISDSEPKA 240

Query: 241 LEAAVKAAVGAPDVLGDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPV 300
           +E AVKAAVGA DV+GDCPF+ RVLLTLEEKKV YK HLIDLSDKPSWFLEVSPEGKVPV
Sbjct: 241 IEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPV 300

Query: 301 VKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQA 360
            K DDKWVPDSDVIVGILEEKYPEP L TPPE SSVGSKI GAFV FL+SKDP+DGSEQA
Sbjct: 301 AKIDDKWVPDSDVIVGILEEKYPEPFLATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQA 360

Query: 361 FLEELKAFNEHLKAHGPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAY 420
            LEELKA NEHLKAHGPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  Y
Sbjct: 361 LLEELKALNEHLKAHGPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKY 420

Query: 421 KETLLASEIACSLKSIMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
           KE                           LLFARESFVKTKAAPEHVI+GWE KVN A
Sbjct: 421 KE---------------------------LLFARESFVKTKAAPEHVISGWEAKVNAA 451

BLAST of Spg009846 vs. ExPASy Swiss-Prot
Match: Q9FRL8 (Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana OX=3702 GN=DHAR2 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 6.0e-85
Identity = 148/211 (70.14%), Postives = 181/211 (85.78%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MA+++  K A GAPDV+GDCPFSQRVLLTLEEK++PY+ HLI++SDKP WFL++SPEGKV
Sbjct: 1   MALDICVKVAVGAPDVLGDCPFSQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K+D KWV DSDVIVG+LEEKYPEPSL TPPEF+SVGSKI GAFV FLKS+D NDGSE
Sbjct: 61  PVVKLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSE 120

Query: 121 QALLEELNALDEHLKAH-GPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSL 180
           +AL++EL AL+ HLK H GP+VAGEK+TAVDLSLAPKLY L++ALGH+K W+ PE+LTS+
Sbjct: 121 KALVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSV 180

Query: 181 SAYKELLFARESFMKTKSAPEHVISGWEPKI 211
             Y + LF+RESF  TK+  E V++GWE K+
Sbjct: 181 RNYAKALFSRESFENTKAKKEIVVAGWESKV 211

BLAST of Spg009846 vs. ExPASy Swiss-Prot
Match: Q65XA0 (Probable glutathione S-transferase DHAR1, cytosolic OS=Oryza sativa subsp. japonica OX=39947 GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.8e-81
Identity = 142/211 (67.30%), Postives = 173/211 (81.99%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           M +EV  KAA G PD +GDCPFSQRVLLTLEEK+VPYE+ LID+ +KP WFL++SPEGKV
Sbjct: 1   MGVEVCVKAAVGHPDTLGDCPFSQRVLLTLEEKKVPYEMKLIDVQNKPDWFLKISPEGKV 60

Query: 61  PVAK-IDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGS 120
           PV    D KW+PDSDVI  ++EEKYP PSLVTPPE++SVGSKI   F  FLKS+DPNDGS
Sbjct: 61  PVFNGGDGKWIPDSDVITQVIEEKYPTPSLVTPPEYASVGSKIFSCFTTFLKSKDPNDGS 120

Query: 121 EQALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSL 180
           E+ALL EL AL+EHLKAHGP++ G+ ++A DLSLAPKLY L +AL HFK W  PE+LT++
Sbjct: 121 EKALLTELQALEEHLKAHGPFINGQNISAADLSLAPKLYHLQVALEHFKGWKIPEDLTNV 180

Query: 181 SAYKELLFARESFMKTKSAPEHVISGWEPKI 211
            AY E LF+RESF+KTK+A EH+I+GW PK+
Sbjct: 181 HAYTEALFSRESFIKTKAAKEHLIAGWAPKV 211

BLAST of Spg009846 vs. ExPASy Swiss-Prot
Match: Q9FWR4 (Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DHAR1 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 6.4e-79
Identity = 141/211 (66.82%), Postives = 171/211 (81.04%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MA+E+  KAA GAPD +GDCPFSQR LLTLEEK + Y++HLI+LSDKP WFL++SP+GKV
Sbjct: 1   MALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV KIDDKWV DSDVIVGILEEKYP+P L TP EF+SVGS I G F  FLKS+D NDGSE
Sbjct: 61  PVLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSE 120

Query: 121 QALLEELNALDEHLKAH-GPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSL 180
            ALL EL AL+ HLK+H GP++AGE+V+AVDLSLAPKLY L +ALGHFK W+ PE+   +
Sbjct: 121 HALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHV 180

Query: 181 SAYKELLFARESFMKTKSAPEHVISGWEPKI 211
             Y + LF+ +SF KTK+  ++VISGW PK+
Sbjct: 181 HNYMKTLFSLDSFEKTKTEEKYVISGWAPKV 211

BLAST of Spg009846 vs. ExPASy Swiss-Prot
Match: Q9FG59 (Probable glutathione S-transferase DHAR4 OS=Arabidopsis thaliana OX=3702 GN=DHAR4 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 5.1e-76
Identity = 141/215 (65.58%), Postives = 175/215 (81.40%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           M IEV  KAA+GAPDV+GDCPF QR+LLTLE+K++PY+ HLID+S KP WFL +SP+GK+
Sbjct: 1   MGIEVCVKAASGAPDVLGDCPFGQRILLTLEDKKLPYKTHLIDVSLKPDWFLAISPKGKL 60

Query: 61  PVAKI--DDKWVPDSDVIVGILEEKYPEPSLVT-PPEFSSVGSKINGAFVKFLKSED-PN 120
           P+ K   D+ WV DSD+IVGI+EEKYPEPSLVT PPEF+SVGSKI GAFV FL S+D  N
Sbjct: 61  PLVKFDEDENWVADSDLIVGIIEEKYPEPSLVTFPPEFASVGSKIIGAFVMFLTSKDHAN 120

Query: 121 DGSEQALLEELNALDEHLKAH-GPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPEN 180
           DGS+ ALL+EL ALD HLK H GP+VAG+KVT VDLSLAPKLY L+  LGHF +W  PE+
Sbjct: 121 DGSDMALLDELEALDHHLKTHVGPFVAGDKVTVVDLSLAPKLYHLETTLGHFMDWCVPES 180

Query: 181 LTSLSAYKELLFARESFMKTKSAPEHVISGWEPKI 211
           LT++  Y ++LF+ ESF KTK+A E++I+ W PK+
Sbjct: 181 LTNVRDYMKVLFSLESFEKTKAAKEYLIASWAPKL 215

BLAST of Spg009846 vs. ExPASy Swiss-Prot
Match: Q67UK9 (Probable glutathione S-transferase DHAR2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=DHAR2 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.5e-72
Identity = 121/208 (58.17%), Postives = 167/208 (80.29%), Query Frame = 0

Query: 3   IEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKVPV 62
           +EV AKA+   PD +GDCPF+QRVLLT+EEK +PY++ L+DL++KP WFL++SPEGKVP+
Sbjct: 63  LEVCAKASLTVPDRLGDCPFTQRVLLTIEEKHLPYDIKLVDLANKPDWFLKISPEGKVPI 122

Query: 63  AKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSEQA 122
            K++++WV DSDVI   +EEKYPEPSL TPPE +SVGSKI   F+ FLKS+DPNDG+EQA
Sbjct: 123 VKLEEQWVADSDVITQAIEEKYPEPSLATPPEKASVGSKIFSTFIGFLKSKDPNDGTEQA 182

Query: 123 LLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLSAY 182
           LL EL + D +LK +GP++ GE ++A DLSLAPKLY ++IALGH+K W+ P++L+ +  Y
Sbjct: 183 LLSELTSFDSYLKDNGPFINGETISAADLSLAPKLYHMEIALGHYKNWSVPDSLSHVKKY 242

Query: 183 KELLFARESFMKTKSAPEHVISGWEPKI 211
            + +F+ +SF+KT +  E VI+GW PK+
Sbjct: 243 MKTIFSMDSFVKTIALQEDVIAGWRPKV 270

BLAST of Spg009846 vs. ExPASy TrEMBL
Match: A0A6J1JER3 (uncharacterized protein LOC111483829 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111483829 PE=3 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.3e-204
Identity = 367/463 (79.27%), Postives = 387/463 (83.59%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAIGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKIQT----LPHCPHDLVLEAAVKAAVGAPDVL 240
            YKELLFARESFMKTK+APEHVISGWEPK+      +        +E AVKAAVGA DV+
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAVPVISDSEPKAIEVAVKAAVGALDVI 240

Query: 241 GDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIV 300
           GDCPF+ RVLLTLEEKKV YK HLIDLSDKP WFLEVSPEGKVPV K DD+WVPDSDVIV
Sbjct: 241 GDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPCWFLEVSPEGKVPVAKIDDRWVPDSDVIV 300

Query: 301 GILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQAFLEELKAFNEHLKAH 360
           GILEEKYPEP L TPPE SSVGSKI GAFV FL+SKDP DGSEQA LEELKA NEHLKAH
Sbjct: 301 GILEEKYPEPFLATPPEFSSVGSKINGAFVKFLKSKDPSDGSEQALLEELKALNEHLKAH 360

Query: 361 GPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAYKETLLASEIACSLKS 420
           GPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  YKE             
Sbjct: 361 GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKE------------- 420

Query: 421 IMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
                         LLFARESFVKTKAAPEHVI+GWE KVN A
Sbjct: 421 --------------LLFARESFVKTKAAPEHVISGWEAKVNDA 436

BLAST of Spg009846 vs. ExPASy TrEMBL
Match: A0A6J1FR13 (uncharacterized protein LOC111447994 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111447994 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.7e-204
Identity = 367/463 (79.27%), Postives = 388/463 (83.80%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWV DSDVIVGILEEKYPEPSLVTPPEFSSVGSK NGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVTPPEFSSVGSKTNGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKI----QTLPHCPHDLVLEAAVKAAVGAPDVL 240
            YKELLFARESFMKTK+APEHVISGWEPK+    + +        +E AVKAAVGA DV+
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAEPVISDSEPKAIEVAVKAAVGALDVI 240

Query: 241 GDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPVVKFDDKWVPDSDVIV 300
           GDCPF+ RVLLTLEEKKV YK HLIDLSDKPSWFLEVSPEGKVPV K DDKWVPDSDVIV
Sbjct: 241 GDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPVAKIDDKWVPDSDVIV 300

Query: 301 GILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQAFLEELKAFNEHLKAH 360
           GILEEKYPEP L TPPE SSVGSKI GAFV FL+SKDP+DGSEQA LEELKA NEHLKAH
Sbjct: 301 GILEEKYPEPFLATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQALLEELKALNEHLKAH 360

Query: 361 GPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAYKETLLASEIACSLKS 420
           GPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  YKE             
Sbjct: 361 GPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKYKE------------- 420

Query: 421 IMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
                         LLFARESFVKTKAAPEHVI+GWE KVN A
Sbjct: 421 --------------LLFARESFVKTKAAPEHVISGWEAKVNAA 436

BLAST of Spg009846 vs. ExPASy TrEMBL
Match: A0A6J1JEQ3 (probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483829 PE=3 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 4.3e-203
Identity = 367/478 (76.78%), Postives = 388/478 (81.17%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAIGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKI-------------------QTLPHCPHDLV 240
            YKELLFARESFMKTK+APEHVISGWEPK+                   + +        
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAVPVISDSEPKVNAATEPVVSDSEPKA 240

Query: 241 LEAAVKAAVGAPDVLGDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPV 300
           +E AVKAAVGA DV+GDCPF+ RVLLTLEEKKV YK HLIDLSDKP WFLEVSPEGKVPV
Sbjct: 241 IEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPCWFLEVSPEGKVPV 300

Query: 301 VKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQA 360
            K DD+WVPDSDVIVGILEEKYPEP L TPPE SSVGSKI GAFV FL+SKDP DGSEQA
Sbjct: 301 AKIDDRWVPDSDVIVGILEEKYPEPFLATPPEFSSVGSKINGAFVKFLKSKDPSDGSEQA 360

Query: 361 FLEELKAFNEHLKAHGPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAY 420
            LEELKA NEHLKAHGPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  Y
Sbjct: 361 LLEELKALNEHLKAHGPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKY 420

Query: 421 KETLLASEIACSLKSIMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
           KE                           LLFARESFVKTKAAPEHVI+GWE KVN A
Sbjct: 421 KE---------------------------LLFARESFVKTKAAPEHVISGWEAKVNDA 451

BLAST of Spg009846 vs. ExPASy TrEMBL
Match: A0A6J1FXI2 (probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447994 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 9.5e-203
Identity = 367/478 (76.78%), Postives = 388/478 (81.17%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAVGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWV DSDVIVGILEEKYPEPSLVTPPEFSSVGSK NGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVSDSDVIVGILEEKYPEPSLVTPPEFSSVGSKTNGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKI-------------------QTLPHCPHDLV 240
            YKELLFARESFMKTK+APEHVISGWEPK+                   + +        
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAEPVISDSEPKVNAAAEPVISDSEPKA 240

Query: 241 LEAAVKAAVGAPDVLGDCPFSHRVLLTLEEKKVAYKMHLIDLSDKPSWFLEVSPEGKVPV 300
           +E AVKAAVGA DV+GDCPF+ RVLLTLEEKKV YK HLIDLSDKPSWFLEVSPEGKVPV
Sbjct: 241 IEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDKPSWFLEVSPEGKVPV 300

Query: 301 VKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEVSSVGSKIFGAFVTFLESKDPDDGSEQA 360
            K DDKWVPDSDVIVGILEEKYPEP L TPPE SSVGSKI GAFV FL+SKDP+DGSEQA
Sbjct: 301 AKIDDKWVPDSDVIVGILEEKYPEPFLATPPEFSSVGSKISGAFVKFLKSKDPNDGSEQA 360

Query: 361 FLEELKAFNEHLKAHGPYVAGEKVTAVDLSLAPKLYHVDVALGHFKKWAIPKDLASLIAY 420
            LEELKA NEHLKAHGPYVAGEKVTAVDLSLAPKLYH+DVALGHFKKW  P++L SL  Y
Sbjct: 361 LLEELKALNEHLKAHGPYVAGEKVTAVDLSLAPKLYHLDVALGHFKKWIFPENLTSLSKY 420

Query: 421 KETLLASEIACSLKSIMLVGFCSTKLEFALLFARESFVKTKAAPEHVIAGWEPKVNPA 460
           KE                           LLFARESFVKTKAAPEHVI+GWE KVN A
Sbjct: 421 KE---------------------------LLFARESFVKTKAAPEHVISGWEAKVNAA 451

BLAST of Spg009846 vs. ExPASy TrEMBL
Match: A0A6J1J9M2 (uncharacterized protein LOC111483829 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483829 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 8.1e-202
Identity = 369/493 (74.85%), Postives = 388/493 (78.70%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MAIEVA KAA GAP+V+GDCPFSQRVLLTLEEK+VPY+LHLI+LSDKP WFLEVSPEGKV
Sbjct: 1   MAIEVAVKAAIGAPNVIGDCPFSQRVLLTLEEKKVPYKLHLINLSDKPIWFLEVSPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K DDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVK LKS+DPNDGSE
Sbjct: 61  PVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKMLKSKDPNDGSE 120

Query: 121 QALLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLS 180
            ALLEEL ALDEHLKAHGPYVAGEKVTAVDLSLAPKLY+LDIALGHFK+W FP NL SLS
Sbjct: 121 LALLEELKALDEHLKAHGPYVAGEKVTAVDLSLAPKLYNLDIALGHFKKWIFPVNLNSLS 180

Query: 181 AYKELLFARESFMKTKSAPEHVISGWEPKI------------------------------ 240
            YKELLFARESFMKTK+APEHVISGWEPK+                              
Sbjct: 181 KYKELLFARESFMKTKAAPEHVISGWEPKVNAAAVPVISDSEPKVNAATEPVVSDSEPKA 240

Query: 241 -QTLPHCPHDL---VLEAAVKAAVGAPDVLGDCPFSHRVLLTLEEKKVAYKMHLIDLSDK 300
                H   D     +E AVKAAVGA DV+GDCPF+ RVLLTLEEKKV YK HLIDLSDK
Sbjct: 241 NSVAEHVVSDCEPKAIEVAVKAAVGALDVIGDCPFAQRVLLTLEEKKVPYKWHLIDLSDK 300

Query: 301 PSWFLEVSPEGKVPVVKFDDKWVPDSDVIVGILEEKYPEPSLVTPPEVSSVGSKIFGAFV 360
           P WFLEVSPEGKVPV K DD+WVPDSDVIVGILEEKYPEP L TPPE SSVGSKI GAFV
Sbjct: 301 PCWFLEVSPEGKVPVAKIDDRWVPDSDVIVGILEEKYPEPFLATPPEFSSVGSKINGAFV 360

Query: 361 TFLESKDPDDGSEQAFLEELKAFNEHLKAHGPYVAGEKVTAVDLSLAPKLYHVDVALGHF 420
            FL+SKDP DGSEQA LEELKA NEHLKAHGPYVAGEKVTAVDLSLAPKLYH+DVALGHF
Sbjct: 361 KFLKSKDPSDGSEQALLEELKALNEHLKAHGPYVAGEKVTAVDLSLAPKLYHLDVALGHF 420

Query: 421 KKWAIPKDLASLIAYKETLLASEIACSLKSIMLVGFCSTKLEFALLFARESFVKTKAAPE 460
           KKW  P++L SL  YKE                           LLFARESFVKTKAAPE
Sbjct: 421 KKWIFPENLTSLSKYKE---------------------------LLFARESFVKTKAAPE 466

BLAST of Spg009846 vs. TAIR 10
Match: AT1G75270.1 (dehydroascorbate reductase 2 )

HSP 1 Score: 316.2 bits (809), Expect = 4.2e-86
Identity = 148/211 (70.14%), Postives = 181/211 (85.78%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MA+++  K A GAPDV+GDCPFSQRVLLTLEEK++PY+ HLI++SDKP WFL++SPEGKV
Sbjct: 1   MALDICVKVAVGAPDVLGDCPFSQRVLLTLEEKKLPYKTHLINVSDKPQWFLDISPEGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV K+D KWV DSDVIVG+LEEKYPEPSL TPPEF+SVGSKI GAFV FLKS+D NDGSE
Sbjct: 61  PVVKLDGKWVADSDVIVGLLEEKYPEPSLKTPPEFASVGSKIFGAFVTFLKSKDANDGSE 120

Query: 121 QALLEELNALDEHLKAH-GPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSL 180
           +AL++EL AL+ HLK H GP+VAGEK+TAVDLSLAPKLY L++ALGH+K W+ PE+LTS+
Sbjct: 121 KALVDELEALENHLKTHSGPFVAGEKITAVDLSLAPKLYHLEVALGHYKNWSVPESLTSV 180

Query: 181 SAYKELLFARESFMKTKSAPEHVISGWEPKI 211
             Y + LF+RESF  TK+  E V++GWE K+
Sbjct: 181 RNYAKALFSRESFENTKAKKEIVVAGWESKV 211

BLAST of Spg009846 vs. TAIR 10
Match: AT1G19570.1 (dehydroascorbate reductase )

HSP 1 Score: 296.2 bits (757), Expect = 4.5e-80
Identity = 141/211 (66.82%), Postives = 171/211 (81.04%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MA+E+  KAA GAPD +GDCPFSQR LLTLEEK + Y++HLI+LSDKP WFL++SP+GKV
Sbjct: 1   MALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQWFLDISPQGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV KIDDKWV DSDVIVGILEEKYP+P L TP EF+SVGS I G F  FLKS+D NDGSE
Sbjct: 61  PVLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSE 120

Query: 121 QALLEELNALDEHLKAH-GPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSL 180
            ALL EL AL+ HLK+H GP++AGE+V+AVDLSLAPKLY L +ALGHFK W+ PE+   +
Sbjct: 121 HALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHV 180

Query: 181 SAYKELLFARESFMKTKSAPEHVISGWEPKI 211
             Y + LF+ +SF KTK+  ++VISGW PK+
Sbjct: 181 HNYMKTLFSLDSFEKTKTEEKYVISGWAPKV 211

BLAST of Spg009846 vs. TAIR 10
Match: AT1G19570.2 (dehydroascorbate reductase )

HSP 1 Score: 287.3 bits (734), Expect = 2.1e-77
Identity = 140/211 (66.35%), Postives = 170/211 (80.57%), Query Frame = 0

Query: 1   MAIEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKV 60
           MA+E+  KAA GAPD +GDCPFSQR LLTLEEK + Y++HLI+LSDKP  FL++SP+GKV
Sbjct: 1   MALEICVKAAVGAPDHLGDCPFSQRALLTLEEKSLTYKIHLINLSDKPQ-FLDISPQGKV 60

Query: 61  PVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSE 120
           PV KIDDKWV DSDVIVGILEEKYP+P L TP EF+SVGS I G F  FLKS+D NDGSE
Sbjct: 61  PVLKIDDKWVTDSDVIVGILEEKYPDPPLKTPAEFASVGSNIFGTFGTFLKSKDSNDGSE 120

Query: 121 QALLEELNALDEHLKAH-GPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSL 180
            ALL EL AL+ HLK+H GP++AGE+V+AVDLSLAPKLY L +ALGHFK W+ PE+   +
Sbjct: 121 HALLVELEALENHLKSHDGPFIAGERVSAVDLSLAPKLYHLQVALGHFKSWSVPESFPHV 180

Query: 181 SAYKELLFARESFMKTKSAPEHVISGWEPKI 211
             Y + LF+ +SF KTK+  ++VISGW PK+
Sbjct: 181 HNYMKTLFSLDSFEKTKTEEKYVISGWAPKV 210

BLAST of Spg009846 vs. TAIR 10
Match: AT5G16710.1 (dehydroascorbate reductase 1 )

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-72
Identity = 121/208 (58.17%), Postives = 162/208 (77.88%), Query Frame = 0

Query: 3   IEVAAKAAAGAPDVMGDCPFSQRVLLTLEEKEVPYELHLIDLSDKPSWFLEVSPEGKVPV 62
           +E+  KA+   P+ +GDCPF Q+VLLT+EEK VPY++ ++DLS+KP WFL++SPEGKVPV
Sbjct: 49  LEICVKASITTPNKLGDCPFCQKVLLTMEEKNVPYDMKMVDLSNKPEWFLKISPEGKVPV 108

Query: 63  AKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSVGSKINGAFVKFLKSEDPNDGSEQA 122
            K D+KWVPDSDVI   LEEKYPEP L TPPE +SVGSKI   FV FLKS+D  DG+EQ 
Sbjct: 109 VKFDEKWVPDSDVITQALEEKYPEPPLATPPEKASVGSKIFSTFVGFLKSKDSGDGTEQV 168

Query: 123 LLEELNALDEHLKAHGPYVAGEKVTAVDLSLAPKLYSLDIALGHFKEWTFPENLTSLSAY 182
           LL+EL   ++++K +GP++ GEK++A DLSLAPKLY + IALGH+K W+ P++L  + +Y
Sbjct: 169 LLDELTTFNDYIKDNGPFINGEKISAADLSLAPKLYHMKIALGHYKNWSVPDSLPFVKSY 228

Query: 183 KELLFARESFMKTKSAPEHVISGWEPKI 211
            E +F+RESF  T++  E VI+GW PK+
Sbjct: 229 MENVFSRESFTNTRAETEDVIAGWRPKV 256

BLAST of Spg009846 vs. TAIR 10
Match: AT1G19550.1 (Glutathione S-transferase family protein )

HSP 1 Score: 171.0 bits (432), Expect = 2.2e-42
Identity = 88/173 (50.87%), Postives = 112/173 (64.74%), Query Frame = 0

Query: 41  LIDLSDKPS--WFLEVSPEGKVPVAKIDDKWVPDSDVIVGILEEKYPEPSLVTPPEFSSV 100
           ++ +   PS   F ++SP+GKVPV KIDDKWV DSD  VGILEEKYP+P L TP EF+SV
Sbjct: 3   VVQICSSPSHEMFWDISPQGKVPVLKIDDKWVTDSDATVGILEEKYPDPPLKTPAEFASV 62

Query: 101 GSKINGAFVKFLKSEDPNDGSEQALLEELNALDEHLKAH-GPYVAGEKVTAVDLSLAPKL 160
           GS I                          AL+ HLK+H GP++AGE+V+AVDLSLAPKL
Sbjct: 63  GSNI------------------------FEALENHLKSHDGPFIAGERVSAVDLSLAPKL 122

Query: 161 YSLDIALGHFKEWTFPENLTSLSAYKELLFARESFMKTKSAPEHVISGWEPKI 211
           Y L +ALGHFK W+ PE+   +  Y + LF+ +SF KTK+  + VISGW PK+
Sbjct: 123 YHLQVALGHFKSWSVPESFPHVHNYMKTLFSLDSFEKTKTEEKCVISGWAPKV 151

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022985954.12.7e-20479.27uncharacterized protein LOC111483829 isoform X3 [Cucurbita maxima][more]
XP_023547271.13.6e-20479.27uncharacterized protein LOC111806131 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022943191.13.6e-20479.27uncharacterized protein LOC111447994 isoform X3 [Cucurbita moschata][more]
XP_022985944.18.8e-20376.78probable glutathione S-transferase DHAR2, chloroplastic isoform X2 [Cucurbita ma... [more]
XP_022943190.12.0e-20276.78probable glutathione S-transferase DHAR2, chloroplastic isoform X2 [Cucurbita mo... [more]
Match NameE-valueIdentityDescription
Q9FRL86.0e-8570.14Glutathione S-transferase DHAR2 OS=Arabidopsis thaliana OX=3702 GN=DHAR2 PE=1 SV... [more]
Q65XA01.8e-8167.30Probable glutathione S-transferase DHAR1, cytosolic OS=Oryza sativa subsp. japon... [more]
Q9FWR46.4e-7966.82Glutathione S-transferase DHAR1, mitochondrial OS=Arabidopsis thaliana OX=3702 G... [more]
Q9FG595.1e-7665.58Probable glutathione S-transferase DHAR4 OS=Arabidopsis thaliana OX=3702 GN=DHAR... [more]
Q67UK91.5e-7258.17Probable glutathione S-transferase DHAR2, chloroplastic OS=Oryza sativa subsp. j... [more]
Match NameE-valueIdentityDescription
A0A6J1JER31.3e-20479.27uncharacterized protein LOC111483829 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FR131.7e-20479.27uncharacterized protein LOC111447994 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JEQ34.3e-20376.78probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita ... [more]
A0A6J1FXI29.5e-20376.78probable glutathione S-transferase DHAR2, chloroplastic isoform X2 OS=Cucurbita ... [more]
A0A6J1J9M28.1e-20274.85uncharacterized protein LOC111483829 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G75270.14.2e-8670.14dehydroascorbate reductase 2 [more]
AT1G19570.14.5e-8066.82dehydroascorbate reductase [more]
AT1G19570.22.1e-7766.35dehydroascorbate reductase [more]
AT5G16710.11.2e-7258.17dehydroascorbate reductase 1 [more]
AT1G19550.12.2e-4250.87Glutathione S-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.1050.10coord: 90..212
e-value: 2.5E-44
score: 152.2
NoneNo IPR availableGENE3D1.20.1050.10coord: 309..459
e-value: 9.8E-39
score: 134.0
NoneNo IPR availablePFAMPF13410GST_C_2coord: 339..394
e-value: 1.2E-6
score: 28.5
coord: 119..179
e-value: 1.8E-6
score: 27.9
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 1..89
e-value: 3.0E-29
score: 102.9
coord: 220..308
e-value: 7.0E-28
score: 98.5
NoneNo IPR availableSFLDSFLDG00358Main_(cytGST)coord: 238..385
e-value: 2.9E-25
score: 83.9
coord: 19..172
e-value: 1.1E-26
score: 88.5
NoneNo IPR availableCDDcd00570GST_N_familycoord: 20..77
e-value: 1.66307E-15
score: 69.1376
NoneNo IPR availableCDDcd00570GST_N_familycoord: 239..296
e-value: 4.39836E-14
score: 64.9004
IPR004045Glutathione S-transferase, N-terminalPFAMPF13417GST_N_3coord: 17..87
e-value: 4.5E-17
score: 62.2
coord: 238..306
e-value: 1.4E-15
score: 57.4
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 229..307
score: 14.34406
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 10..88
score: 16.324059
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 19..172
e-value: 1.1E-26
score: 88.5
coord: 238..385
e-value: 2.9E-25
score: 83.9
IPR044627Dehydroascorbate reductases DHAR1/2/3/4PANTHERPTHR44420GLUTATHIONE S-TRANSFERASE DHAR2-RELATEDcoord: 2..209
coord: 222..455
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 66..212
score: 10.049634
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 18..87
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 238..306
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 97..210
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 316..408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009846.1Spg009846.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033355 ascorbate glutathione cycle
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0006749 glutathione metabolic process
molecular_function GO:0045174 glutathione dehydrogenase (ascorbate) activity
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding