Spg009779 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg009779 vs. NCBI nr
Match: XP_038892136.1 (probable dual-specificity RNA methyltransferase RlmN [Benincasa hispida])

HSP 1 Score: 783.5 bits (2022), Expect = 1.1e-222
Identity = 398/415 (95.90%), Postives = 407/415 (98.07%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MI ISMAL+HQVCS SVARA+RPRCVAAVPSRNLSTSISPP RASPE PKVLIGLS+QEL
Sbjct: 2   MIPISMALIHQVCSPSVARALRPRCVAAVPSRNLSTSISPPARASPEAPKVLIGLSDQEL 61

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLAVDFGQEKYRGKQL+QLIYKRKVKEIEEFTNLP AFRNDLQEAGW+VGRSPVYQSVT
Sbjct: 62  QQLAVDFGQEKYRGKQLHQLIYKRKVKEIEEFTNLPLAFRNDLQEAGWKVGRSPVYQSVT 121

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK+NKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 122 ASDGTVKLLLKLDDNRLIETVGIPVEDKNNKGSVRLTACISSQVGCPLRCSFCATGKGGF 181

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI
Sbjct: 182 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 241

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+MKDCRDYFLQ
Sbjct: 242 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEALMKDCRDYFLQ 301

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVEL+KLLHEWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 302 TSRRVSFEYALLAGVNDAVEHAVELSKLLHEWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 361

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVAVA 416
           AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL D+D SQSESAVAVA
Sbjct: 362 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLVDSDISQSESAVAVA 416

BLAST of Spg009779 vs. NCBI nr
Match: XP_022989725.1 (uncharacterized protein LOC111486721 [Cucurbita maxima])

HSP 1 Score: 776.2 bits (2003), Expect = 1.7e-220
Identity = 396/415 (95.42%), Postives = 404/415 (97.35%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MITISMAL+HQVCS SVARA+RPRCVAAVPSR  STSISPP RASPE PKVLIGLSE+EL
Sbjct: 1   MITISMALIHQVCSPSVARALRPRCVAAVPSRKSSTSISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLAVD GQEKYRGKQL+QLIYKRKVKE+EEFTNLP AFRN LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVAVA 416
           AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL DTDSSQSESAVAVA
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSESAVAVA 415

BLAST of Spg009779 vs. NCBI nr
Match: XP_022957587.1 (uncharacterized protein LOC111458934 [Cucurbita moschata])

HSP 1 Score: 772.7 bits (1994), Expect = 1.9e-219
Identity = 393/415 (94.70%), Postives = 403/415 (97.11%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MI +SMAL+HQVCS SVARA+RPRCVAAVPSR  STSISPP RASPE PKVLIGLSE+EL
Sbjct: 1   MIAVSMALIHQVCSPSVARALRPRCVAAVPSRKSSTSISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLAVD GQEKYRGKQL+QLIYKRKVKE+EEFTNLP AFRN LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYF+Q
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFVQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVAVA 416
           AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL DTDSSQSESAVAVA
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSESAVAVA 415

BLAST of Spg009779 vs. NCBI nr
Match: KAG7031617.1 (rlmN, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 770.8 bits (1989), Expect = 7.3e-219
Identity = 393/415 (94.70%), Postives = 402/415 (96.87%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MI ISMAL+HQVCS SVARA+RPRCVAAVPSR  ST ISPP RASPE PKVLIGLSE+EL
Sbjct: 1   MIAISMALIHQVCSPSVARALRPRCVAAVPSRKSSTFISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLAVD GQEKYRGKQL+QLIYKRKVKE+EEFTNLP AFRN LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYF+Q
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFVQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVAVA 416
           AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL DTDSSQSESAVAVA
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSESAVAVA 415

BLAST of Spg009779 vs. NCBI nr
Match: KAG6601003.1 (hypothetical protein SDJN03_06236, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 768.8 bits (1984), Expect = 2.8e-218
Identity = 392/415 (94.46%), Postives = 401/415 (96.63%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MI ISMAL+HQVCS SVARA+RPRCVAAVPSR  ST  SPP RASPE PKVLIGLSE+EL
Sbjct: 1   MIAISMALIHQVCSPSVARALRPRCVAAVPSRKSSTFTSPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLAVD GQEKYRGKQL+QLIYKRKVKE+EEFTNLP AFRN LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYF+Q
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFVQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVAVA 416
           AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL DTDSSQSESAVAVA
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSESAVAVA 415

BLAST of Spg009779 vs. ExPASy Swiss-Prot
Match: Q2JRQ8 (Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=rlmN PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 2.0e-102
Identity = 195/350 (55.71%), Postives = 250/350 (71.43%), Query Frame = 0

Query: 48  PPKVLIGLSEQELQQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAG 107
           PP  L+G S   L+  AV+ GQ  YRG+QL+  +Y + ++ ++E T  P+A+R  LQE  
Sbjct: 4   PPLPLLGQSLAALKDWAVEQGQPAYRGQQLHTWLYHKGIRSLQEVTVFPKAWREALQE-- 63

Query: 108 WRVGRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCP 167
           + VGRS V Q + + DGTVK LL+L D  LIETVGIP        + RLT C+SSQVGCP
Sbjct: 64  YPVGRSQVVQRIESRDGTVKFLLQLADGELIETVGIPT-------ARRLTVCVSSQVGCP 123

Query: 168 LRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHR 227
           + C+FCATGK G+ RNL+ HEI++QV  +++ F  RV++VVFMGMGEP+LN  +V++A R
Sbjct: 124 MACNFCATGKMGYRRNLKLHEILDQVLTVQEDFGRRVSHVVFMGMGEPLLNRDTVVQAIR 183

Query: 228 CLNKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPL 287
            LN+D+ IGQR IT+STVGVP  I  LA   LQ TLAVSLHAPNQ+LR+ ++PSA  YPL
Sbjct: 184 SLNQDIGIGQRHITLSTVGVPRQIPWLAQQDLQITLAVSLHAPNQELRQQLIPSAAHYPL 243

Query: 288 EAIMKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLH---EWGRGYHVNLIPFN 347
           +A+++DCRDY L + RR+SFEY LLAGVND   HA +LA LL    + G   HVNLIP+N
Sbjct: 244 DALIQDCRDYMLCSGRRISFEYTLLAGVNDLPIHARQLAHLLRQASQAGARLHVNLIPYN 303

Query: 348 PIEGSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQF 395
           PI  ++YQRP+   V  FV  LE H + VSVRQTRGLD+NAACGQLR  F
Sbjct: 304 PIAEADYQRPHPTRVAEFVRLLEEHHVQVSVRQTRGLDSNAACGQLRGSF 344

BLAST of Spg009779 vs. ExPASy Swiss-Prot
Match: Q2JMN2 (Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=rlmN PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.9e-100
Identity = 191/352 (54.26%), Postives = 247/352 (70.17%), Query Frame = 0

Query: 49  PKVLIGLSEQELQQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGW 108
           P  L+G S   L+  AV  GQ  YRG+QL+  IY++ ++ +E+ T  P+A+R  +Q   +
Sbjct: 5   PIPLLGQSLSALKDWAVAQGQPAYRGQQLHAWIYQKGIRSLEQVTVFPRAWREAVQ--SY 64

Query: 109 RVGRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPL 168
            VGRS + Q   A DGTVK LL L D +LIETVGIP        + RLT C+SSQVGCP+
Sbjct: 65  PVGRSRIVQRTEARDGTVKFLLGLADGQLIETVGIPT-------AKRLTVCVSSQVGCPM 124

Query: 169 RCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRC 228
            C FCATGK G+ RNL+ HEI++QV  +++ F  RV++VVFMGMGEP+LN  +V++A R 
Sbjct: 125 ACDFCATGKMGYRRNLELHEILDQVLTVQEDFGRRVSHVVFMGMGEPLLNRDTVVQAIRS 184

Query: 229 LNKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLE 288
           LN+D+ IGQR IT+STVGVP  I  LA   LQ TLAVSLHAPNQ LR+ ++PSA  YPL+
Sbjct: 185 LNQDIGIGQRHITLSTVGVPRQIAWLAQQDLQVTLAVSLHAPNQDLRQRLIPSASHYPLD 244

Query: 289 AIMKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRG---YHVNLIPFNP 348
            +++DCRDY L+T RRVSFEY LL+GVND   HA +LA+LL +  R     HVNLIP+NP
Sbjct: 245 TLIQDCRDYMLRTGRRVSFEYTLLSGVNDLPIHARQLAQLLQQASRSGVQLHVNLIPYNP 304

Query: 349 IEGSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKN 398
           I  ++YQRP+   V  FV  LE H++  +VRQTRGLD NAACGQLR  F ++
Sbjct: 305 ISEADYQRPHPTRVREFVRQLEQHQVRATVRQTRGLDGNAACGQLRGSFLRS 347

BLAST of Spg009779 vs. ExPASy Swiss-Prot
Match: B0C9F4 (Probable dual-specificity RNA methyltransferase RlmN OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=rlmN PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.0e-98
Identity = 188/344 (54.65%), Postives = 244/344 (70.93%), Query Frame = 0

Query: 48  PPKVLIGLSEQELQQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAG 107
           PP  L+G S+ +L   A  + Q  YRG+Q++Q IY++ V  + + T LP+ +R ++  A 
Sbjct: 11  PP--LLGASKAQLTHWAQTYQQPAYRGQQVHQWIYQKGVHSLSDITVLPKQWRTEI--AD 70

Query: 108 WRVGRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCP 167
             VGRS ++    A DGTVK LLKL D ++IETVGIP +        RLT C+SSQVGCP
Sbjct: 71  IPVGRSQIHHRSAAQDGTVKYLLKLADGQIIETVGIPTQK-------RLTVCVSSQVGCP 130

Query: 168 LRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHR 227
           + C FCATGKG + RNL  HEIV+QV  +++ F  RV NVVFMGMGEP+LN++ VL A R
Sbjct: 131 MGCDFCATGKGEYQRNLACHEIVDQVLTVQEDFQQRVGNVVFMGMGEPLLNLEQVLAAVR 190

Query: 228 CLNKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPL 287
            LN+DV IGQR +T+STVG+P  I +LA H+LQ TLAVSLHA NQ++R  +VPSAK YPL
Sbjct: 191 SLNQDVGIGQRSLTVSTVGIPKQILKLAQHQLQITLAVSLHASNQRIRTQLVPSAKHYPL 250

Query: 288 EAIMKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIE 347
           E ++KDCR Y  QT RRV+FEY +L+GVND  EHA+ELA   H  G   HVNLIP+NPI 
Sbjct: 251 EKLLKDCRAYVTQTGRRVTFEYIVLSGVNDQTEHALELAH--HLRGFQSHVNLIPYNPIS 310

Query: 348 GSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLR 392
             +YQRP +K +  F+ +L+S  IT S+R++RGLD +AACGQLR
Sbjct: 311 EVDYQRPTQKQLQQFLNSLQSQHITASIRRSRGLDKDAACGQLR 341

BLAST of Spg009779 vs. ExPASy Swiss-Prot
Match: Q8DG98 (Probable dual-specificity RNA methyltransferase RlmN OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=rlmN PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.9e-97
Identity = 185/341 (54.25%), Postives = 239/341 (70.09%), Query Frame = 0

Query: 51  VLIGLSEQELQQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRV 110
           VL+G S  EL+      GQ  YRG+QL+Q +Y++  + ++E T  P+ +R  L +    +
Sbjct: 2   VLLGRSAAELKAWVEAQGQPGYRGQQLHQWLYRKGARSLQEITVFPKQWRAALADV--EI 61

Query: 111 GRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRC 170
           GRS +     A DGTVKLLL L D   IETVGIP  D       RLT C+SSQVGCP+ C
Sbjct: 62  GRSEIRYRHDAQDGTVKLLLALADGETIETVGIPSSD-------RLTVCVSSQVGCPMAC 121

Query: 171 SFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLN 230
            FCATGKGG+ RNL  HEI++QV  I+     RV++VVFMGMGEP+LN+ +VL+A  CLN
Sbjct: 122 DFCATGKGGYRRNLACHEILDQVLTIQSEMGRRVSHVVFMGMGEPLLNLPAVLQAITCLN 181

Query: 231 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 290
           +D+ IGQR ITISTVG+P  I+RLA H+LQ+TLAVSLHAPNQ LRE ++PSAK YPL  +
Sbjct: 182 RDIGIGQRHITISTVGIPQQIQRLAQHQLQTTLAVSLHAPNQALREQLIPSAKHYPLSQL 241

Query: 291 MKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSE 350
           + DCR Y  QT RR++FEY +LAGVND  +HA ELA+LL   G   HVNLIP+NPI  + 
Sbjct: 242 IADCRAYVQQTGRRITFEYTVLAGVNDRPQHAEELAQLLR--GFQSHVNLIPYNPIAEAA 301

Query: 351 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLR 392
           YQRP  + +  F++ L++  +T S+R++RGLD  AACGQLR
Sbjct: 302 YQRPTDQHLRQFLSQLQALGVTASIRRSRGLDRQAACGQLR 331

BLAST of Spg009779 vs. ExPASy Swiss-Prot
Match: B1XQH8 (Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=rlmN PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 4.3e-97
Identity = 185/350 (52.86%), Postives = 243/350 (69.43%), Query Frame = 0

Query: 48  PPKVLIGLSEQELQQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAG 107
           P  VL+G S  EL       GQ  YRGKQLY  +Y++ + ++ E T  P+A+R   Q   
Sbjct: 2   PQDVLLGKSLPELTDWIETTGQPAYRGKQLYNWLYQKGIHDLSEITVFPKAWRE--QMGT 61

Query: 108 WRVGRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCP 167
           + VGRS ++   TA DGT K LL+L D  +IETVGIP E        RLT C+SSQVGC 
Sbjct: 62  YPVGRSQIHHQRTAPDGTRKYLLQLHDGLIIETVGIPTEK-------RLTVCVSSQVGCA 121

Query: 168 LRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHR 227
           + C FCATGK GF+R+LQ HEI++QV  ++  F  RV++VVFMGMGEP+ N++ VL++ +
Sbjct: 122 MACDFCATGKSGFTRHLQAHEIIDQVLTVQTDFQQRVSHVVFMGMGEPLANLEQVLKSIQ 181

Query: 228 CLNKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPL 287
            LN+D+ IGQR +T+STVGVP+ I+ LA   LQ TLAVSLHAPNQ LRE+I+P+A  YP+
Sbjct: 182 SLNQDIGIGQRSLTVSTVGVPDQIRALAQQNLQITLAVSLHAPNQALRESIIPTAVHYPI 241

Query: 288 EAIMKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIE 347
           EA++ +CR+Y   T RR+SFEY LLAGVND  +HA ELAK L   G   HVNLIP+NPI 
Sbjct: 242 EALLDECREYVAITRRRLSFEYILLAGVNDLPDHAAELAKKLK--GFQSHVNLIPYNPIT 301

Query: 348 GSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKN 398
              +QRP KK +  F   L+ HKI VSVR ++GL+A+AACGQLR+  +++
Sbjct: 302 EVPFQRPGKKRINVFKQILQDHKIAVSVRYSKGLEADAACGQLRSNLRRS 340

BLAST of Spg009779 vs. ExPASy TrEMBL
Match: A0A6J1JN65 (uncharacterized protein LOC111486721 OS=Cucurbita maxima OX=3661 GN=LOC111486721 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 8.4e-221
Identity = 396/415 (95.42%), Postives = 404/415 (97.35%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MITISMAL+HQVCS SVARA+RPRCVAAVPSR  STSISPP RASPE PKVLIGLSE+EL
Sbjct: 1   MITISMALIHQVCSPSVARALRPRCVAAVPSRKSSTSISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLAVD GQEKYRGKQL+QLIYKRKVKE+EEFTNLP AFRN LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVAVA 416
           AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL DTDSSQSESAVAVA
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSESAVAVA 415

BLAST of Spg009779 vs. ExPASy TrEMBL
Match: A0A6J1GZJ0 (uncharacterized protein LOC111458934 OS=Cucurbita moschata OX=3662 GN=LOC111458934 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 9.2e-220
Identity = 393/415 (94.70%), Postives = 403/415 (97.11%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MI +SMAL+HQVCS SVARA+RPRCVAAVPSR  STSISPP RASPE PKVLIGLSE+EL
Sbjct: 1   MIAVSMALIHQVCSPSVARALRPRCVAAVPSRKSSTSISPPARASPESPKVLIGLSEEEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLAVD GQEKYRGKQL+QLIYKRKVKE+EEFTNLP AFRN LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLAVDLGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDK++KGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKNSKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIE+IFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEEIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYF+Q
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFVQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLL EWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVAVA 416
           AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL DTDSSQSESAVAVA
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSESAVAVA 415

BLAST of Spg009779 vs. ExPASy TrEMBL
Match: A0A0A0KLM9 (Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G181470 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 2.1e-216
Identity = 386/413 (93.46%), Postives = 398/413 (96.37%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MI IS AL+HQVCS SVARA+RPRCVAAVPSRNLSTSISP  R SPE PKVLIGLS+QEL
Sbjct: 1   MIAISTALIHQVCSPSVARALRPRCVAAVPSRNLSTSISPSARPSPEAPKVLIGLSDQEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLA+DFGQEKYRGKQL+QLIYKRKVKE+EEFTNLP  FR DLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLALDFGQEKYRGKQLHQLIYKRKVKEVEEFTNLPLKFRTDLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           A+DGTVKLLLKLDDNRLIETVGIPVEDK+NKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 AADGTVKLLLKLDDNRLIETVGIPVEDKNNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTI+RLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+MKDCRDYFLQ
Sbjct: 241 TISTVGVPNTIQRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEALMKDCRDYFLQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVA 414
           AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL D+D SQ E+A A
Sbjct: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLVDSDISQPEAAEA 413

BLAST of Spg009779 vs. ExPASy TrEMBL
Match: A0A1S3CGI7 (probable dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=LOC103500206 PE=3 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.8e-215
Identity = 386/415 (93.01%), Postives = 398/415 (95.90%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MI IS AL+HQVCS SVARA+R RCVAAVPSRNLSTSISP    SPE PKVLIGLS+QEL
Sbjct: 1   MIAISTALIHQVCSPSVARALRSRCVAAVPSRNLSTSISPSAEPSPEAPKVLIGLSDQEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLA+DFGQEKYRGK L+QLIYKRKVKEIEEFTNLP  FRNDLQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLALDFGQEKYRGKLLHQLIYKRKVKEIEEFTNLPLKFRNDLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           A+DGTVKLLLKLDDNRLIETVGIPVEDK+NKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 AADGTVKLLLKLDDNRLIETVGIPVEDKNNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+MKDC DYFLQ
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEALMKDCHDYFLQ 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWG GYHVNLIPFNPIEGSEYQRPYKKAVM
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPIEGSEYQRPYKKAVM 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSESAVAVA 416
           AFVAALESHKITVSVR+TRGLDANAACGQLRNQFQKNPLL D+D SQSE+A AVA
Sbjct: 361 AFVAALESHKITVSVRRTRGLDANAACGQLRNQFQKNPLLVDSDISQSEAAEAVA 415

BLAST of Spg009779 vs. ExPASy TrEMBL
Match: A0A6J1CCN0 (uncharacterized protein LOC111010274 OS=Momordica charantia OX=3673 GN=LOC111010274 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 1.2e-214
Identity = 388/416 (93.27%), Postives = 400/416 (96.15%), Query Frame = 0

Query: 1   MITISMALLHQVCSTSVARAVRPRCVAAVPSRNLSTSISPPTRASPEPPKVLIGLSEQEL 60
           MI ISMAL+H VCS S ARAVRPR VAAVPSRNLSTS SP  R  PE  KVLIGLSEQEL
Sbjct: 1   MIAISMALIHHVCSXSAARAVRPRRVAAVPSRNLSTSTSPRARPPPEASKVLIGLSEQEL 60

Query: 61  QQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVT 120
           QQLA+DFGQEKYRGKQL+QLIYKRKVKEIEEFTNLPQAFRN LQEAGWRVGRSPVYQSVT
Sbjct: 61  QQLALDFGQEKYRGKQLHQLIYKRKVKEIEEFTNLPQAFRNGLQEAGWRVGRSPVYQSVT 120

Query: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180
           ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF
Sbjct: 121 ASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGF 180

Query: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDVQIGQRMI 240
           SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAH+CLNKDVQIGQRMI
Sbjct: 181 SRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHQCLNKDVQIGQRMI 240

Query: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQ 300
           TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFL+
Sbjct: 241 TISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLE 300

Query: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVM 360
           TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAV+
Sbjct: 301 TSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVV 360

Query: 361 AFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQS-ESAVAVA 416
           AFVAALES KITVSVRQTRGLDANAACGQLRNQFQK+PL+ D+DS++S ESAVA+A
Sbjct: 361 AFVAALESRKITVSVRQTRGLDANAACGQLRNQFQKDPLVADSDSNRSDESAVAMA 416

BLAST of Spg009779 vs. TAIR 10
Match: AT2G39670.1 (Radical SAM superfamily protein )

HSP 1 Score: 604.0 bits (1556), Expect = 1.1e-172
Identity = 312/423 (73.76%), Postives = 359/423 (84.87%), Query Frame = 0

Query: 3   TISMALLHQ-VCSTSVAR-AVRPRCVAAVPSRNLSTSISPPTRASPEPPK-------VLI 62
           T +MA+L   V S  ++R  VR   +A   S   +T +  P   S +P +       VLI
Sbjct: 6   TNTMAMLQNLVFSVPISRMVVRRHSLATTFSAAATTVVPSPKPVSAKPARTPHVDSHVLI 65

Query: 63  GLSEQELQQLAVDFGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRVGRS 122
           G+SE ELQ+LA++ GQE YRGKQL+ LIYKRKV ++E+F+NLP  FR  L + G++VGRS
Sbjct: 66  GMSEPELQELAINLGQEGYRGKQLHHLIYKRKVNKVEDFSNLPLTFRKGLVDGGFKVGRS 125

Query: 123 PVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRCSFC 182
           P+YQ+VTA+DGT+KLLLKL+DN LIETVGIPV+D D KG  RLTAC+SSQVGCPLRCSFC
Sbjct: 126 PIYQTVTATDGTIKLLLKLEDNLLIETVGIPVQD-DEKGITRLTACVSSQVGCPLRCSFC 185

Query: 183 ATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLNKDV 242
           ATGKGGFSRNLQRHEI+EQV AIED+F HRVTNVVFMGMGEPMLN+KSVL+AHRCLNKD+
Sbjct: 186 ATGKGGFSRNLQRHEIIEQVLAIEDVFKHRVTNVVFMGMGEPMLNLKSVLDAHRCLNKDI 245

Query: 243 QIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKD 302
           +IGQRMITISTVGVPNTIK+LASHKLQSTLAVSLHAPNQ LRE IVPSAKAYPLEAIMKD
Sbjct: 246 EIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAIMKD 305

Query: 303 CRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQR 362
           CRDYF +T+RRVSFEYALLAGVND VEHAVELA+LL EWG+ YHVNLIP+NPIEGSEYQR
Sbjct: 306 CRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSEYQR 365

Query: 363 PYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSE-SAV 416
           PYKKAV+AF AALES KIT SVRQTRGLDA+AACGQLRN+FQK+PLLT+TDS +S+  A 
Sbjct: 366 PYKKAVLAFAAALESRKITASVRQTRGLDASAACGQLRNKFQKSPLLTETDSQESQPDAE 425

BLAST of Spg009779 vs. TAIR 10
Match: AT2G39670.2 (Radical SAM superfamily protein )

HSP 1 Score: 595.1 bits (1533), Expect = 5.1e-170
Identity = 311/426 (73.00%), Postives = 358/426 (84.04%), Query Frame = 0

Query: 3   TISMALLHQ-VCSTSVAR-AVRPRCVAAVPSRNLSTSISPPTRASPEPPK-------VLI 62
           T +MA+L   V S  ++R  VR   +A   S   +T +  P   S +P +       VLI
Sbjct: 6   TNTMAMLQNLVFSVPISRMVVRRHSLATTFSAAATTVVPSPKPVSAKPARTPHVDSHVLI 65

Query: 63  GLSEQELQQLAVD---FGQEKYRGKQLYQLIYKRKVKEIEEFTNLPQAFRNDLQEAGWRV 122
           G+SE ELQ+LA++     QE YRGKQL+ LIYKRKV ++E+F+NLP  FR  L + G++V
Sbjct: 66  GMSEPELQELAINLVLIFQEGYRGKQLHHLIYKRKVNKVEDFSNLPLTFRKGLVDGGFKV 125

Query: 123 GRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIPVEDKDNKGSVRLTACISSQVGCPLRC 182
           GRSP+YQ+VTA+DGT+KLLLKL+DN LIETVGIPV+D D KG  RLTAC+SSQVGCPLRC
Sbjct: 126 GRSPIYQTVTATDGTIKLLLKLEDNLLIETVGIPVQD-DEKGITRLTACVSSQVGCPLRC 185

Query: 183 SFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRVTNVVFMGMGEPMLNMKSVLEAHRCLN 242
           SFCATGKGGFSRNLQRHEI+EQV AIED+F HRVTNVVFMGMGEPMLN+KSVL+AHRCLN
Sbjct: 186 SFCATGKGGFSRNLQRHEIIEQVLAIEDVFKHRVTNVVFMGMGEPMLNLKSVLDAHRCLN 245

Query: 243 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 302
           KD++IGQRMITISTVGVPNTIK+LASHKLQSTLAVSLHAPNQ LRE IVPSAKAYPLEAI
Sbjct: 246 KDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAI 305

Query: 303 MKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGRGYHVNLIPFNPIEGSE 362
           MKDCRDYF +T+RRVSFEYALLAGVND VEHAVELA+LL EWG+ YHVNLIP+NPIEGSE
Sbjct: 306 MKDCRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSE 365

Query: 363 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSE- 416
           YQRPYKKAV+AF AALES KIT SVRQTRGLDA+AACGQLRN+FQK+PLLT+TDS +S+ 
Sbjct: 366 YQRPYKKAVLAFAAALESRKITASVRQTRGLDASAACGQLRNKFQKSPLLTETDSQESQP 425

BLAST of Spg009779 vs. TAIR 10
Match: AT3G19630.1 (Radical SAM superfamily protein )

HSP 1 Score: 194.9 bits (494), Expect = 1.5e-49
Identity = 123/312 (39.42%), Postives = 183/312 (58.65%), Query Frame = 0

Query: 90  EEFTNLPQAFRNDLQEAGWRVGRSPVYQSVTASDGTV-KLLLKLDDNRLIETVGIPVE-- 149
           +E  +LP A    L  + ++   S ++    +SDGT  KLL+KL +   +E V +  +  
Sbjct: 42  DEIPSLPSA-AYSLLHSKFKTLTSSLHSLFHSSDGTTSKLLIKLQNGAFVEAVVMRYDTR 101

Query: 150 -----DKDNKGSVRLTACISSQVGCPLRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFN 209
                 K   G +R T CISSQVGC + C+FCATG  GF  NL   EIVEQ+     I +
Sbjct: 102 LGMLGGKPRPGGIRSTLCISSQVGCKMGCTFCATGTMGFKSNLTSGEIVEQLVHASRIAD 161

Query: 210 HRVTNVVFMGMGEPMLNMKSVLEAHR-CLNKDVQIGQRMITISTVGVPNTIKRLASHKLQ 269
             + N+VFMGMGEP+ N  +V+EA R  LN+  Q+  + ITISTVG+ + I +L +    
Sbjct: 162 --IRNIVFMGMGEPLNNYNAVVEAVRVMLNQPFQLSPKRITISTVGIVHAINKLHNDLPG 221

Query: 270 STLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQTSRRVSFEYALLAGVNDAVE 329
            +LAVSLHAP Q++R  I+P+A+A+PL+ +M   + +   + +++  EY +L GVND  +
Sbjct: 222 VSLAVSLHAPVQEIRCQIMPAARAFPLQKLMDALQTFQKNSQQKIFIEYIMLDGVNDQEQ 281

Query: 330 HAVELAKLLHEWGRGYHVNLIPFNPI-EGSEYQRPYKKAVMAFVAAL-ESHKITVSVRQT 389
           HA  L +LL  +     +NLIPFNPI   S+++    + V  F   L E++KI  ++R+ 
Sbjct: 282 HAHLLGELLKTF--QVVINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKE 341

Query: 390 RGLDANAACGQL 391
            G D + ACGQL
Sbjct: 342 MGQDISGACGQL 348

BLAST of Spg009779 vs. TAIR 10
Match: AT1G60230.1 (Radical SAM superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.8e-45
Identity = 128/372 (34.41%), Postives = 190/372 (51.08%), Query Frame = 0

Query: 27  AAVPSRNLSTSISPPTRASPEPPKVLIGLSEQELQQLAVDFGQEKYRGKQLYQLIYKRKV 86
           A +PS     S     +       VL G++   LQ+     G    +   L++ +YK  +
Sbjct: 72  AYLPSLEEYPSAKGSVKRDDNQKVVLKGMTYASLQEWVQSHGFRPGQALMLWKRLYKDNI 131

Query: 87  --KEIEEFTNLPQAFRNDLQEAGWRVGRSPVYQSVTASDGTVKLLLKLDDNRLIETVGIP 146
               ++E   L +  +  + E     G        +ASDGT K+L  LDD  +IETV IP
Sbjct: 132 WANNVDELEGLNKDLKRMISEHA-EFGALSFKDIRSASDGTRKILFTLDDGLVIETVVIP 191

Query: 147 VEDKDNKGSVRLTACISSQVGCPLRCSFCATGKGGFSRNLQRHEIVEQVFAIEDIFNHRV 206
            +    +G  R T C+SSQVGC + C FC TG+ G  RNL   EIVEQ      + +H V
Sbjct: 192 CD----RG--RTTVCVSSQVGCAMNCQFCYTGRMGLKRNLTTAEIVEQAVYARRLLSHEV 251

Query: 207 ---TNVVFMGMGEPMLNMKSVLEAHRCL--NKDVQIGQRMITISTVGVPNTIKRLASHKL 266
              TNVVFMGMGEP  N+ +V++A   +     +    R +T+ST G+   +KR    + 
Sbjct: 252 GSITNVVFMGMGEPFHNIDNVIKAANIMVDENGLHFSPRKVTVSTSGLVPQLKRFL-RES 311

Query: 267 QSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQTSR-RVSFEYALLAGVNDA 326
              LAVSL+A   ++R  I+P  + Y L  +++  R+      + +V FEY +LAGVND+
Sbjct: 312 NCALAVSLNATTDEVRNWIMPINRKYKLSLLLETLREGLSSRHKYKVLFEYVMLAGVNDS 371

Query: 327 VEHAVELAKLLHEWGRGYHVNLIPFNPIEGSEYQRPYKKAVMAFVAALESHKITVSVRQT 386
           ++ A  L +L+   G    +NLI FNP  GS++ +  +  ++ F   L     TV +R +
Sbjct: 372 MDDARRLVELVQ--GIPCKINLIQFNPHSGSQFIQTEEDKMIKFRNVLAEGGCTVLMRFS 431

Query: 387 RGLDANAACGQL 391
           RG D  AACGQL
Sbjct: 432 RGNDQMAACGQL 433

BLAST of Spg009779 vs. TAIR 10
Match: AT1G50050.1 (CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 7.7e-09
Identity = 30/47 (63.83%), Postives = 36/47 (76.60%), Query Frame = 0

Query: 364 AALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLTDTDSSQSES 411
           AAL+S KIT SVRQTRGL A+AACGQLRN+FQK+P L   +    E+
Sbjct: 154 AALKSRKITASVRQTRGLGASAACGQLRNKFQKSPSLATAEGEVIEA 200