Spg009758 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009758
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncrossover junction endonuclease MUS81
Locationscaffold7: 6230541 .. 6238300 (+)
RNA-Seq ExpressionSpg009758
SyntenySpg009758
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTTGATGAACTAAAAAAAACCCTTGATTTTATTTCGTAAGAAATTCAACCCGAATTCAAAATCGCGAGCCTCGCGCGGTAATGTGGCCTTCTTCTTTCCCTCTTCCCGTATCTCGTTTCTTCACAATTCCCCAACTATTTTCCATCTTCTTCTTCTTCTAAACTTCGAATCAAAGAAATGGACCGCCAAAGATTGCCGGTCGTCTGTCCGGAGAATGAAGAACTTGCGGCGTTTATGTGGCAGAAGCGGCTAGAAATGGCTGCGACACCCAAAGGAATTTCTGACAAAGTCGATATGACTCTGTACAAAGCTTATTTCAATGTCTGCAACTCCAAGGCTTCCATCAAATCCATGAAGGATCTCTCCCAGATCAAGTATGTTTTGCATCCCCTCCCTCACTGTTCCTGCATTGTTATGTGGTTTTAGAATCTGTCTGGTGATGCAATTGAGTGGTTGAATGCGAATGCTGTTTAGTCTCTTTTCTGTTTTCATTGGGTTCTTTCTGTTGGATGGCAATTCTGTTTCTATTCTGTAATGCAATGTTATATCACTGATTATGCTTAATAAAGGCATGGTTGAAGTTTGTTAGTCTCGAATTGAAGCTGGAAGGTCTGGGTGACAACGAAGGTCTTTGTTTGTTTGTTTTAGCAGTTATGTTGGGATTTTTCTCTGTCATTTTCAATATTTTTTTGGGTTATTCTGAATAATAATGGGACTTTCTTTCTTTCAGGTAGCTTAGTAGTTGAAAGAGTTGTATTTTTCTTTTTATCCTCCTTAATTATCATTCATTAGAATGTTTAAAATAGTTACAGATTGTCTTTCATTCTTTGAATGCACTGTCGCATTAAAGTGGGATTTCTGGCTTCTACTCTCTCTTTTGAAGTATTAATGTTCTAATGCCACAGAGGCGTAGGGAAGTGGATTCTTAAACAAGTACAAGATTTTTTTAAGAGTGGTTCTGAAGGTTCTGAACCTGAAGACTTGGCGGGAAAAGGTACTTCACACAAGCTCGGCGTTCTAAGTTTTGATGGTTTACTTATGGTTTGTCATACAAAGAAATATTTCTTATTGTTTTACCTTTTTCCTGTTTATTCATTTAGGTAAGAGAGCAAAAGGAACAAAACGCTATGTTCCACAGAAGAACTCAGTTGCATATGCCTTATTGGTTACTCTTTATAGGTACAATGGACAAATTGCAATTTCTAATGTCGAGTTCTTATTTGAACTTATATGTAATGTACGTTTCATTATTAGGAGTACATCAAATGGGAATGAATACATGCGTAAACAGGAGCTTATTGATGCAGCTGAAGCAAGTGGTCTTTCTAGGGTGCCAATTGGGTAAGTTAATTCCTCTATTCTTCCTTATGTCTTACATTGCACCACTTAATGGATGGAGACATTGGAGAGTATTTTCACGTTTTCAGGCCAGAAAAAGGTAAAGGGAAACCTAGTCAATTTGGAAGCTCTCCAAGGGATTGGTACAGTGGATGGAGCTGCATGAAGACATTGATATCTAGGGGATTGGTGGTGAAATCTAGTTGCCCAGCAAAGTATGGTCTTGGTTTGGTCATAATTTTTTTCATTGCCTCTTTCCCTCACTCTAATTCTTTTACAATGATTTTCTCTATGTATCATCACGTCATTGCTTCCATGGCTGGGGATGGGTACCATCTGATTGTCAAGGACTTTTACTGATGGTTGTGGGCTGCTCTTTTGTTTACTTAGGAGTTAGAAATAATGTACAAGGACCATCTGACCTATGCAACATGTTAATATATGTGAAGGCACATATTTTAAATTCTTTAAGTGGTGGATATTTTATTCTTGAACCTACTTATATGTACTATGTTTCTGCAATATTAGAAGTCTTAATTTATAGCTTATTGTTCAATTATTAATGAGTGCAAATGTGTATTTGTCAATATCATGTAATTTTTCTTGCATTGACAAAAGCATGTGGTAGGGAAATGTGAGCATAACTTAATTGGTTAAAGCATATACTCTCGGTCAAGAGGTTCGAGGTTCAAATCTCTCAAAAAAAGACAAAAGCATGTGGTAGCTTCAGCTTGATTCTTTAAGCTTTAGAAGTTGGAATATGAAATAATGGCTCGTTACATAAGTATTTTGCAAGTATGGAATCGCACAACTCTGACTATCTAGCAAAGACTATTACCGTTCATAAAGATTAATCCTGCAAAGCTTTAAGGGAAAAATACTATGAGACTTAACTTGCACTAGAATATCACTTTAACTGTGCTTAACTTTGCAACTTAAAATATCATGAACGCTAATAGTAGTTTTATTCAAACAGTGACATCAATGTTCATATGTGGATTATTTCTCATAGAATTAAAACAAGAAATCAATATTCCATATCCTGGTCATTTTTTTTTTTATAAATATTTTGTTTTTCTGCATAAGCCATGTTTGAAGAATATTACAGATTAGGATCAATCTTCTTGTTCTATTATTTGCAAGTATATGCTAATATTTTTGTTTTTACAGATACATGTTAACAGAGGAGGGGCAACAAGTGGCACGTGAATGTCTTATGAGATCTGGATTGTTAGATTCTACCACGAACTTTGATAATGCCAAGGGATCCTCTGTTCTGGAATCATGTGATTTATCTGATCGCAATGCTGTTAATCTTGATTCAGATGTGGAAGTCACTTCCCCACCTGTTGGTCAGAGCAGGCAGAACAAATCAACTGATGTTCCCCTTGAATCACTTGAGAGGGTAATGCAACGCTTTAGATGTCACATTTTTGTTTGTATAATTCACTTGATATGGTTTATAACTTAAGCAATATGCAATTTGAGTATTCACAATTACATTCATGGATTATCTATGACACTAGGGCTGCATAATATATACAGCCAACTGGCTGTATCGATAGCCATTGTTAGTTTGATTTCCATTTGTTCTTCACCAAAGCCCCAAGCCCAACCCAACTGGCAACCCCTTGTGGGCAATTCACCATCTATTTCTGGTTCTTTTTTGTTTTTCTTTTTCTCCAGTTTATGCGAATGGGCTACTCCAAGCATCAAGTTCTCAATGCCTTCAAAGAAGTTTCAGAAGATAACCCGTACAAGGATATCTCATCACTTTGGCCAGCAGTATTATGTCGTCTTCGTGAAGATCTTGTTTATGGTTTGCTCTTAAAATTCTTAGCAGGAAAATTAGCATATTGCCACAACATTAGATTAGGTGCTAGAAAACCAAACCAAATATCGTCATAAGCTTTTTTTTATCAGAAAAGGAGAAATGGTTATAAGCTTTTTGATAACATAAGCCTGTTTATTTCTCCAACTATGAAGGAGTTGGAGTAGTCGTTTTCCTTTTTTATTATTGAAGAATACTGACCACTAGATTGATAAGTTGTATATATACACAGGTCAAGTAGAAGCAGTTGGAAGAGAAAGTAGGTCGATGGGTTCTTCTCATGAGGGGCATATGCCAAAGACTGTTGTTATTGATGATGGGGAGAGATCTTTTAACTTGAGAGCTTGCTCATCATCTGTAAGTGGATATTCTAGCTTGTTCATTCTCCCAATGTTCTTAATGCAAAAAAAGTATGTCATTAGAAAATTGGTAGTGATTTTTTCTTTTTGCAATGGGTTTATTTTTGTATTATGGAGCAATGAATCTTTATTGTAATTTGAATTTATAGAATCATGCTGTCACTGAATCCTGTAGAGATGATGTCAAAGCAAATATGAACATTTTGAGCATGCCACCTTTGAGCTTTGGTGAGAAATTCGAGGATTTGTATAACGTAGTCTTGATATTGGATGATCGAGAACAGTTTGCCAACCATAGGTCAGTGATTTTGATTTATAAAATAACATATTTCCAGAAATCTGAGGTTTGCACCTTTTTTTTAACCTCTCTTGACTAGACTGGAGCTGTTGCACCTTTTTGCATTTTAGCTTTAACAAACAATGCTCATGTCTTGTTCATCAATCAATGTAAAGTTTAAACGTTTTTGTTTCTTATCATTAATTAATAAAAATTCTTATCATGTTAAAAAAAAAATTTTAAAAAAAAGTGCTCGTATCCTGGCTTGGACCTTTGGTCTACCCTCCTCCTTTTCACAGCTATCACAAGTTTCCTTTTAAAAAACCATATGGGCTTGATTTCGTAAATTTTAGCCAGGGACGTTATTGATTTTGTGATACATGTGAAAGACAAAACTACAAATCAAAGACTCATGAAAGCTTGTATTCCCTGACGTGTCATGTGCATGCTTGTATCTATGATGACATTAGGGGTACCTTTTTGCAAACTTGCAGTTGTTTATTGATATTTAACCTATTGTTATCTAGTTGTGTTTTATTTACTGCTAAACTATGTACATCTTTTGTGGTTTTCTAGTTCTCGGTCTAGGAGAATGATTGAGAATGTCTGTAGCCAATTTAAGATCCAAATTGAGGTAACTTCACTCTGTTTTTCTGTGAAAGTTTCTTTTTCATAATACATTGCTATGTTATTAGGTTATCAATTAAATTTGATAGGTATTAAGAGTTTGCCATCTACAACAACATTTTTCAGCACTTTTGATTAACTTTTTGAATAATACCATATTTTTCCCCTAATGAACTTGGATAAAATGATTTGTCTTCAAAAGTAAAAATGAACATCCACTATTCAACACAGAACATGTAGCCCACAATGAGAAATAGCTTATTAAAATATGTGTTTTAGCAATTTCATGTTGCCACATATGTTTATTCTTGTCAGCAGTTCTCCTATTTCCATAATCAAACTATTTACTTGGTTATGATGACTGACTCACTCTTTTTCTTCAGATTAGGAGGCTACCTGTTGGTGACGGAATCTGGATAGCTCGCCATAAACATCTTGATAATGAATATGTTCTTGATTTTATAGTTGAGAGAAAAAACATTGATGATTTGCGGTGCTCAATCAGGGACAACCGCTATAGAGACCAAAAATTGAAACTTCTGGTAACTTTTTAACTAATTGATATTTTTGTTTTTCTATTTCCCTATTGTGGCAGCTGCATTCTGTCAATTTATGCTATGGATTTGTTGAGAAATAGGATTGTAACCTGTCACTACTTCCATTCCAAAAGTGGGTCTTGTTATTAATATGTTCCCATTTCTATAGCTTTAGCTGACAGCTCTTGGTCCCTCCTCCCCAATGTATCCTCACTTTTATACTTTCATTCTTCTCGATGGAATTTTGGTTCTTATTTATTAAAAAAGAAGAAAAGAAAAAACTGCAGCAACACTGCTACTGTTATTTTCAAATTGCCACCGAGGGATTTTCTGTTAGATTCGGTTTCAATTAGCCCAAATTTTATGGAGACTACAAGGATGTATCGTGTGTATAGCCAATGTGATTCTCAAAAATGACAAGGGTTTGTTGAAAATTGGTAGTAAATATTGGACTAAGTGAGCTCAAATGCCAAAGTCCATTTGTTATTATTATTTGGCAAGAATATTATAGGCAGATTACTATAAGGGTGCTGATCTTTGCTTATTTATTTATCTTTGTAGCTAGTTTCTTACTAATTAATGCTTCTTACTGTATCTCATTAGAGATGTGGGCTTAAAAAGATGATATATCTTGTGGAAGGTGATCCCAATTCATCTGAAGCGGCAGAAAGCATCAAAACAGCGTAAGTGTGGTTCTTCTTGTTCCTTATTAGTGGTCTCTAGATTATAAGTAGTGGTTCATGGAAAAATGCTCTTTTATAATACCTGTAGTGAAAAAAGATGCTCTTTGGCTCTAGAAAGGACATTATCTAGCTATGGTTAAATACTGTAGCGTGAGAGGGGGCAGAGCATCTTTTGCCATTTTGGCAAGTTTGTTATTAGGAGCGAATAGGGAGCTCTTGAATTCTCTTTGGCTCTGTTTATCTCTAATTATCGGTAATAAAAGAGGTTCTATCACTTTTCAAAAAAAAAAAAAAGAAAAAAAAGAGGTTCTATCATATACTTTATATTGTACGATTAATTTTCCTCAGTTTTTTTTTGATAGTTTTGCATCTGGTTCTCATTGAATTCTTTCTCCCTCGAAGGTCCATGTATATTTTTTTTTTCTCGGCCTATCTAGTAATTCATGAAAAGCAATTCTAAAAGTAATTTTCCCCCTTAAACTGTGGTATATTTTATGTTTTGCAGTTGCTTTACAACAGAGATTTTAGAGGGATTTGATGTGCAAAGAACCAGTGGCTTGGCTGATACACTAAAGAAGTATGGTTATCTTACTCGTGCAATTACACAATACTACATATCACAGGAAACTGACGAGCGTCTTAGTCATTCTGATGTCTGTCCTCGTTTCAATGAATTTATCAAAAAGTGCCAAGAATTGGATAAGATGACGGTTAGTGATGTTTTTGCACTTCAGCTCATGCAGGTATGAACAATTTTATGAAGGAATGCGAAGTTCGTTCTCAAAAGTCTACCATGATTTAAAATCTAATGGCATGCACGTATACAGGTCCCGCAGGTTACGGAGGAGGTTGCCGTTGCTGTTTTAGATTTGTACCCAACTCTTCTATCACTTGCCCGAGCATACTCTCTTCTAGTGAGTTCAATTTTCCGTTTCCTTCTATTTCATAAGGTTGCTCATTTTTCATTTGATTACATTGCTTATTGGGAATTGGGATAGGTTTAAGACTTTTCTTTTAATATCTATAAGTGTCGGGGCTGGCTTGGGCACACTTCGGCTGTTCTCCTGGGACAATATTTTAAAACATTTGTTATGGGTGAAAGGAATAAAGTTCCCTTCTTCATTGTTTCTTATTCAAGAAATAAAGTTCTCATCTTCATACTCTAGGATAGTTTTCTTCAAGTGTAAAGATAATCTACTTACCTATCATGGAATTGGTTTCTTTTGGCCTAGTAGTAACCTTAATTTGAAAACTGTATAATTGCCAAAAACGGTTAATCTACTCAAACATAAGACTAAGAGTACTTCTTCTAGACAACATTATCAGCATTTTTAGTGAGATGATGTATAGGTTAGTTAACCTTTAGGAGAATGAGTTTAGGTCATGGTATCACCTACCTAAAATTTAATATTCTACGAGTTGCTTGGCAACCAAATGTAGTAAGGCCTAACGATTGTCCTGTGGGATTAGTCAGAAGTGCGTGCAAGTTGGCCTAGACACTCATATATGTATTATCACTTAGAGGGTGTCTGGTTCACGACTGCATAAATCCATGTTTTATAAATTTAGGCTTATAAAACCCATCTTCTATATTACATACAAACATCTATAAACTACAAATCCAAAAACAAGTAAAGAATACACAAATCCAAATCTGTATAACTTATTCCTTGTAAAATGAAAACACATATTATAAAAACATAGGTTTTTTAACTAAATAAAAATGTGGGCTAAAATAAGAAAATCGTTTATTTGTGTGTATTTGTCAAGTAATTTATATTCTTTTTTTCTTTTTTAAATGAAAAAACTAGGACGGTAATGTTACTGAACAAGAAGAGATGCTACGGAGACAGAGCAACAATTTGATCAATGCAGGTGCTAGCCGGAATATTTTTCATTTAGTATGGGGTTGTGGGAAGTAGTTAGCATATATCTGCAATTCACAATAAACCTGCCTTTTATGACCTTGATGGCAGTTAGTTTCATAGTTACGGCTGTAAATATGCTTATTATTCATTTCAAATGCCATGTTCATATGATATAGTTGTGCTCAGAGTTGATTATGATTGTACAATTTGATGAAACTAAATAAGCTGCTTAGTTTCTG

mRNA sequence

ATGGACCGCCAAAGATTGCCGGTCGTCTGTCCGGAGAATGAAGAACTTGCGGCGTTTATGTGGCAGAAGCGGCTAGAAATGGCTGCGACACCCAAAGGAATTTCTGACAAAGTCGATATGACTCTGTACAAAGCTTATTTCAATGTCTGCAACTCCAAGGCTTCCATCAAATCCATGAAGGATCTCTCCCAGATCAAAGGCGTAGGGAAGTGGATTCTTAAACAAGTACAAGATTTTTTTAAGAGTGGTTCTGAAGGTTCTGAACCTGAAGACTTGGCGGGAAAAGGTAAGAGAGCAAAAGGAACAAAACGCTATGTTCCACAGAAGAACTCAGTTGCATATGCCTTATTGGTTACTCTTTATAGGTACAATGGACAAATTGCAATTTCTAATGTCGAGTTCTTATTTGAACTTATATGTAATGTACGTTTCATTATTAGGAGTACATCAAATGGGAATGAATACATGCGTAAACAGGAGCTTATTGATGCAGCTGAAGCAAGTGGTCTTTCTAGGGTGCCAATTGGGCCAGAAAAAGGTAAAGGGAAACCTAGTCAATTTGGAAGCTCTCCAAGGGATTGGTACAGTGGATGGAGCTGCATGAAGACATTGATATCTAGGGGATTGGTGGTGAAATCTAGTTGCCCAGCAAAATACATGTTAACAGAGGAGGGGCAACAAGTGGCACGTGAATGTCTTATGAGATCTGGATTGTTAGATTCTACCACGAACTTTGATAATGCCAAGGGATCCTCTGTTCTGGAATCATGTGATTTATCTGATCGCAATGCTGTTAATCTTGATTCAGATGTGGAAGTCACTTCCCCACCTGTTGGTCAGAGCAGGCAGAACAAATCAACTGATGTTCCCCTTGAATCACTTGAGAGGTTTATGCGAATGGGCTACTCCAAGCATCAAGTTCTCAATGCCTTCAAAGAAGTTTCAGAAGATAACCCGTACAAGGATATCTCATCACTTTGGCCAGCAGTATTATGTCGTCTTCGTGAAGATCTTGTTTATGGTCAAGTAGAAGCAGTTGGAAGAGAAAGTAGGTCGATGGGTTCTTCTCATGAGGGGCATATGCCAAAGACTGTTGTTATTGATGATGGGGAGAGATCTTTTAACTTGAGAGCTTGCTCATCATCTAATCATGCTGTCACTGAATCCTGTAGAGATGATGTCAAAGCAAATATGAACATTTTGAGCATGCCACCTTTGAGCTTTGGTGAGAAATTCGAGGATTTGTATAACGTAGTCTTGATATTGGATGATCGAGAACAGTTTGCCAACCATAGTTCTCGGTCTAGGAGAATGATTGAGAATGTCTGTAGCCAATTTAAGATCCAAATTGAGATTAGGAGGCTACCTGTTGGTGACGGAATCTGGATAGCTCGCCATAAACATCTTGATAATGAATATGTTCTTGATTTTATAGTTGAGAGAAAAAACATTGATGATTTGCGGTGCTCAATCAGGGACAACCGCTATAGAGACCAAAAATTGAAACTTCTGAGATGTGGGCTTAAAAAGATGATATATCTTGTGGAAGGTGATCCCAATTCATCTGAAGCGGCAGAAAGCATCAAAACAGCTTGCTTTACAACAGAGATTTTAGAGGGATTTGATGTGCAAAGAACCAGTGGCTTGGCTGATACACTAAAGAAGTATGGTTATCTTACTCGTGCAATTACACAATACTACATATCACAGGAAACTGACGAGCGTCTTAGTCATTCTGATGTCTGTCCTCGTTTCAATGAATTTATCAAAAAGTGCCAAGAATTGGATAAGATGACGGTTAGTGATGTTTTTGCACTTCAGCTCATGCAGGTCCCGCAGGTTACGGAGGAGGTTGCCGTTGCTGTTTTAGATTTGTACCCAACTCTTCTATCACTTGCCCGAGCATACTCTCTTCTAGACGGTAATGTTACTGAACAAGAAGAGATGCTACGGAGACAGAGCAACAATTTGATCAATGCAGGTGCTAGCCGGAATATTTTTCATTTAGTATGGGGTTGTGGGAAGTAG

Coding sequence (CDS)

ATGGACCGCCAAAGATTGCCGGTCGTCTGTCCGGAGAATGAAGAACTTGCGGCGTTTATGTGGCAGAAGCGGCTAGAAATGGCTGCGACACCCAAAGGAATTTCTGACAAAGTCGATATGACTCTGTACAAAGCTTATTTCAATGTCTGCAACTCCAAGGCTTCCATCAAATCCATGAAGGATCTCTCCCAGATCAAAGGCGTAGGGAAGTGGATTCTTAAACAAGTACAAGATTTTTTTAAGAGTGGTTCTGAAGGTTCTGAACCTGAAGACTTGGCGGGAAAAGGTAAGAGAGCAAAAGGAACAAAACGCTATGTTCCACAGAAGAACTCAGTTGCATATGCCTTATTGGTTACTCTTTATAGGTACAATGGACAAATTGCAATTTCTAATGTCGAGTTCTTATTTGAACTTATATGTAATGTACGTTTCATTATTAGGAGTACATCAAATGGGAATGAATACATGCGTAAACAGGAGCTTATTGATGCAGCTGAAGCAAGTGGTCTTTCTAGGGTGCCAATTGGGCCAGAAAAAGGTAAAGGGAAACCTAGTCAATTTGGAAGCTCTCCAAGGGATTGGTACAGTGGATGGAGCTGCATGAAGACATTGATATCTAGGGGATTGGTGGTGAAATCTAGTTGCCCAGCAAAATACATGTTAACAGAGGAGGGGCAACAAGTGGCACGTGAATGTCTTATGAGATCTGGATTGTTAGATTCTACCACGAACTTTGATAATGCCAAGGGATCCTCTGTTCTGGAATCATGTGATTTATCTGATCGCAATGCTGTTAATCTTGATTCAGATGTGGAAGTCACTTCCCCACCTGTTGGTCAGAGCAGGCAGAACAAATCAACTGATGTTCCCCTTGAATCACTTGAGAGGTTTATGCGAATGGGCTACTCCAAGCATCAAGTTCTCAATGCCTTCAAAGAAGTTTCAGAAGATAACCCGTACAAGGATATCTCATCACTTTGGCCAGCAGTATTATGTCGTCTTCGTGAAGATCTTGTTTATGGTCAAGTAGAAGCAGTTGGAAGAGAAAGTAGGTCGATGGGTTCTTCTCATGAGGGGCATATGCCAAAGACTGTTGTTATTGATGATGGGGAGAGATCTTTTAACTTGAGAGCTTGCTCATCATCTAATCATGCTGTCACTGAATCCTGTAGAGATGATGTCAAAGCAAATATGAACATTTTGAGCATGCCACCTTTGAGCTTTGGTGAGAAATTCGAGGATTTGTATAACGTAGTCTTGATATTGGATGATCGAGAACAGTTTGCCAACCATAGTTCTCGGTCTAGGAGAATGATTGAGAATGTCTGTAGCCAATTTAAGATCCAAATTGAGATTAGGAGGCTACCTGTTGGTGACGGAATCTGGATAGCTCGCCATAAACATCTTGATAATGAATATGTTCTTGATTTTATAGTTGAGAGAAAAAACATTGATGATTTGCGGTGCTCAATCAGGGACAACCGCTATAGAGACCAAAAATTGAAACTTCTGAGATGTGGGCTTAAAAAGATGATATATCTTGTGGAAGGTGATCCCAATTCATCTGAAGCGGCAGAAAGCATCAAAACAGCTTGCTTTACAACAGAGATTTTAGAGGGATTTGATGTGCAAAGAACCAGTGGCTTGGCTGATACACTAAAGAAGTATGGTTATCTTACTCGTGCAATTACACAATACTACATATCACAGGAAACTGACGAGCGTCTTAGTCATTCTGATGTCTGTCCTCGTTTCAATGAATTTATCAAAAAGTGCCAAGAATTGGATAAGATGACGGTTAGTGATGTTTTTGCACTTCAGCTCATGCAGGTCCCGCAGGTTACGGAGGAGGTTGCCGTTGCTGTTTTAGATTTGTACCCAACTCTTCTATCACTTGCCCGAGCATACTCTCTTCTAGACGGTAATGTTACTGAACAAGAAGAGATGCTACGGAGACAGAGCAACAATTTGATCAATGCAGGTGCTAGCCGGAATATTTTTCATTTAGTATGGGGTTGTGGGAAGTAG

Protein sequence

MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMKDLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLDSTTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGHMPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCGK
Homology
BLAST of Spg009758 vs. NCBI nr
Match: XP_038892703.1 (crossover junction endonuclease MUS81 isoform X1 [Benincasa hispida])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 587/678 (86.58%), Postives = 615/678 (90.71%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MDRQR PVVCPENE++A FMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSK  IKSMK
Sbjct: 1   MDRQRSPVVCPENEDIATFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKVPIKSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQV DFFK+GSEGS PE+L GKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61  DLSQIKGVGKWILKQVHDFFKNGSEGSGPEELVGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDA+EASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDASEASGLSRVAIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKS+CPAKYMLTEEGQQVARECLMRSGLL 
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           ST N DN KGSS+LESCD+SD +A +LDSDVEVTSP   +S QNKSTDVPLESLERFMRM
Sbjct: 241 STKNVDNTKGSSILESCDISDHSANSLDSDVEVTSPTAIKSSQNKSTDVPLESLERFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESR--SMGSSHE 360
           GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+R  S  SSH+
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRLTSSSSSHD 360

Query: 361 GHMPKTVVIDDGERSFNLRACSSSNHAV-TESCRDDVKANMNILSMPPLSFGEKFEDLYN 420
            + PK VV D+GER FNLRACSSSN ++ +ESCRD VKANMNILSMPPLSFGEKFED YN
Sbjct: 361 RNGPKIVVNDNGERFFNLRACSSSNPSISSESCRDGVKANMNILSMPPLSFGEKFEDSYN 420

Query: 421 VVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF 480
           VVLILDDREQFANHSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF
Sbjct: 421 VVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF 480

Query: 481 IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEI 540
           IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEI
Sbjct: 481 IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEI 540

Query: 541 LEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDK 600
           LEGFDVQRTSGL+DTLKKYGYLTRAITQYYI Q +DERLSHSDVCPR NEFIKKCQELDK
Sbjct: 541 LEGFDVQRTSGLSDTLKKYGYLTRAITQYYILQASDERLSHSDVCPRLNEFIKKCQELDK 600

Query: 601 MTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNL 660
           MTVSDVFALQLMQVPQVTEEVAVAVL+LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNL
Sbjct: 601 MTVSDVFALQLMQVPQVTEEVAVAVLELYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNL 653

Query: 661 INAGASRNIFHLVWGCGK 676
           INAGASRNIFHLVWGCGK
Sbjct: 661 INAGASRNIFHLVWGCGK 653

BLAST of Spg009758 vs. NCBI nr
Match: XP_022956424.1 (crossover junction endonuclease MUS81 [Cucurbita moschata])

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 576/675 (85.33%), Postives = 611/675 (90.52%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQVQ  FK+GSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61  DLSQIKGVGKWILKQVQVIFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKP QFGSS RDWYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           S  NFD  KGSS+LESC +SDR+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCGISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
           GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+RS GSS+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTGSSNEGH 360

Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
           +PK VV D+GERSFNLRACSSSNH++TES +D VK N NILSMPPLSFGEKFED Y+VVL
Sbjct: 361 VPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSVVL 420

Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
           ILDDREQFANHSSRSRRMIEN+CSQFKI+IEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIEIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480

Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
           RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540

Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
           FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DERLSH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTV 600

Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
           SDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINA 650

Query: 661 GASRNIFHLVWGCGK 676
            ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 650

BLAST of Spg009758 vs. NCBI nr
Match: XP_023514312.1 (crossover junction endonuclease MUS81 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 577/675 (85.48%), Postives = 612/675 (90.67%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQVQ FFK+GSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61  DLSQIKGVGKWILKQVQVFFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKP QFGSS RDWYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           S  NFD  KGSS+LESCD+SDR+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCDISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
           GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+RS G S+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTG-SNEGH 360

Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
           +PK VV D+GERSFNLRACSSSNH++TES +D VK N NILSMPPLSFGEKFED Y++VL
Sbjct: 361 VPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSIVL 420

Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
           ILDDREQFANHSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480

Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
           RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540

Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
           FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DERLSH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTV 600

Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
           SDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINA 649

Query: 661 GASRNIFHLVWGCGK 676
            ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 649

BLAST of Spg009758 vs. NCBI nr
Match: XP_022992270.1 (crossover junction endonuclease MUS81 [Cucurbita maxima])

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 574/675 (85.04%), Postives = 608/675 (90.07%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKG SDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGTSDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQVQ FFKSGSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61  DLSQIKGVGKWILKQVQVFFKSGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKP QFGSS R+WYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTREWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           S  NFD  KGSS+LESCD+S R+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCDISGRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
           GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+R  GSS+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRLTGSSNEGH 360

Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
           +PK V+ D+GERSFNLRACSSSN ++TESC+D VK NMNILSMPPLSFGEKFED Y+VVL
Sbjct: 361 VPKIVISDNGERSFNLRACSSSNQSITESCKDVVKENMNILSMPPLSFGEKFEDAYSVVL 420

Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
           ILDDREQFANHSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480

Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
           RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540

Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
           FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DER SH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERPSHFDVCPRLDAFIKKCQELDKMTV 600

Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
           SDVFALQLMQVPQVTEE+AV VLDLYPTLLSLARAYSLLD NVT QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVTVLDLYPTLLSLARAYSLLDDNVTHQEEMLRRQSNNLINA 650

Query: 661 GASRNIFHLVWGCGK 676
            ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 650

BLAST of Spg009758 vs. NCBI nr
Match: XP_008446914.1 (PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo] >XP_008446915.1 PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo])

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 575/675 (85.19%), Postives = 610/675 (90.37%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MDRQR PV CPENE+LAAFMWQKRLEMAATPKGISDKVDMTLYKAYFN+CNSK SI+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQVQDFFKSGSEGS PE+LAGKGK+ KGT+RYVPQKNSVAYALL+TL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKS+CPAKYMLTEEGQQVARECLMRSGLL 
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           S+ NFDN KGSS+LESCD+S+ +A  LDSDVEVTSP V Q  +NK+ DVPLESLERFMRM
Sbjct: 241 SSKNFDNIKGSSILESCDISNHSANYLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
           GYSKHQV+N+FKEVS+DNP KD SSLWPAVLCRLREDLVYGQVEAVGRE+RSMG SHEG 
Sbjct: 301 GYSKHQVINSFKEVSKDNPNKDTSSLWPAVLCRLREDLVYGQVEAVGRENRSMG-SHEGS 360

Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
            PK VV D+GERSFN RACSSSN ++ ES RD VK + NILSMPPLSFGEKFED YNVVL
Sbjct: 361 EPKNVVNDNGERSFNSRACSSSNPSIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVVL 420

Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
           ILDDREQFA HSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIVE
Sbjct: 421 ILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIVE 480

Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
           RK+IDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKDIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540

Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
           FDVQRTSGL+DTLKKYGYLT AITQYYI Q +D+R+SHSDVCPR ++FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTV 600

Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
           SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 649

Query: 661 GASRNIFHLVWGCGK 676
           GASRNIFHLVWG G+
Sbjct: 661 GASRNIFHLVWGSGR 649

BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match: Q5W9E7 (Crossover junction endonuclease MUS81 OS=Arabidopsis thaliana OX=3702 GN=MUS81 PE=1 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 9.7e-193
Identity = 366/694 (52.74%), Postives = 472/694 (68.01%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MD +R  V+CPEN  LAA++ QK+ E A  PKG+S+ ++ T  K Y +VC++K  I ++K
Sbjct: 1   MDDER-RVLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGK--GKRAKGTKRYVPQKNSVAYALLV 120
           DLSQIKG GKW++K ++ +F +  E SE EDL     GK+A G KRY+PQ+NSV YALL+
Sbjct: 61  DLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLI 120

Query: 121 TLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPE 180
           TL+                         R T+NG E+MRKQELIDAA+A+GLS  P+GPE
Sbjct: 121 TLH-------------------------RRTTNGKEFMRKQELIDAADANGLSHAPVGPE 180

Query: 181 KGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGL 240
           KGKGK +  G S R+WYSGWSCM TLI +GLVVKSS PAKYMLT EG++VA EC++RSGL
Sbjct: 181 KGKGK-AGLGHSKREWYSGWSCMTTLIQKGLVVKSSNPAKYMLTVEGREVANECILRSGL 240

Query: 241 LDSTTNFDNAKGSSVLESCDLSDRN---AVNLDSDVEVTSPPVGQSRQNKSTDVPLESLE 300
            DS       +     ++    ++N   +  +  ++    P     R    + +P + LE
Sbjct: 241 PDSVDILSVDEMDPTPQAKKTPNQNPTCSFTMREELPYVDP-----RCRAQSAIPSDILE 300

Query: 301 RFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGS 360
           +F   GYSK QV+ AF+EVS+ +  KD S+LW +V+C LR+  VY        +SR+   
Sbjct: 301 KFTPFGYSKEQVVAAFREVSDGSGDKDPSTLWLSVMCHLRQAEVYNSCP----DSRNSKK 360

Query: 361 SHEGHMPKTV--VIDDGERSFNLRAC----------SSSNHAV----TESCRDDVKANMN 420
              G     +  V  +G R+   R+C          S S+HAV    +    D  K   N
Sbjct: 361 DSSGPFKSQIRQVDLEGSRAKKFRSCNDGSTLNPCSSGSSHAVKACSSSLASDGTKGITN 420

Query: 421 ILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGD 480
           I  +PPL FGE FE+ Y+V+LILDDRE+FA   SRSR ++EN+CS+F I+IE+RRLPVGD
Sbjct: 421 IPRLPPLQFGETFEEAYDVILILDDREKFATKGSRSRNIVENICSEFNIKIEVRRLPVGD 480

Query: 481 GIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDP 540
            IWIARHK+L+ EYVLDFI ERKN+DD+R SIRDNRYRDQKL+L R G KK+IY++EGDP
Sbjct: 481 CIWIARHKYLETEYVLDFIAERKNVDDMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDP 540

Query: 541 NSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHS 600
           N S+AAESIKTACFTTEILEGFDV RT GL +TL+KYGYLT++I QYY  +  D   S  
Sbjct: 541 NHSDAAESIKTACFTTEILEGFDVLRTHGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKG 600

Query: 601 DV-CPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSL 660
              CP F+ F+K+CQ+LDKMT+SDVFA+QLMQVPQVTEE+A+AVLD+YPTLLSLA AYS 
Sbjct: 601 AASCPSFDSFVKRCQDLDKMTISDVFAIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSH 658

Query: 661 LDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWG 673
           L+ +V+ QEEMLR +SNN+I A AS+NIF LVWG
Sbjct: 661 LEADVSAQEEMLRNRSNNVICASASKNIFKLVWG 658

BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match: Q8GT06 (Crossover junction endonuclease MUS81 OS=Oryza sativa subsp. japonica OX=39947 GN=MUS81 PE=1 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 2.9e-152
Identity = 328/700 (46.86%), Postives = 428/700 (61.14%), Query Frame = 0

Query: 2   DRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMKD 61
           + ++L V   ENE +A  + +K   M   P G+ + +  TLYK+Y NVC +K  I+S+KD
Sbjct: 4   EARQLKVHLRENEAVAQCVLEKWRSMEEKPGGLKENLAHTLYKSYRNVCAAKEPIRSLKD 63

Query: 62  LSQIKGVGKWILKQVQDFF-KSGSEGSEPEDLAG--KGKRAKGTKRYVPQKNSVAYALLV 121
           L QIKGVGKW+++Q++  F +S  + S PE  A   KGK+A G+KRYVPQKNS AYA+L+
Sbjct: 64  LYQIKGVGKWVIRQLKGSFPESSPDLSPPESNAAGEKGKKAGGSKRYVPQKNSAAYAILI 123

Query: 122 TLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPE 181
           TL+                         R T NG  +M+KQELIDA EASGLSR  IGP+
Sbjct: 124 TLH-------------------------RETINGKSHMKKQELIDATEASGLSRSAIGPD 183

Query: 182 KGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGL 241
           K K KP  F SS +DWY+GWSCMKTL S+GLV KS  PAKYM+TEEG+  A ECL RSGL
Sbjct: 184 KSKAKPGAFASSQKDWYTGWSCMKTLTSKGLVAKSGNPAKYMITEEGKSTALECLSRSGL 243

Query: 242 LDSTTNFDNAKGSSVLESCDLSDR-NAVNLDSDVEVTSPP---VGQ---SRQNKSTDVPL 301
            D          +S  ++ + S + N + + S VE +S P   +G+   S  N +T    
Sbjct: 244 DDHAAPL---VINSAPDTSNASHKLNNICMTSFVETSSGPSRAIGRPKTSIANPATKTSP 303

Query: 302 ESLERFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESR 361
           E      +   + +  +   +  +E+    D  S          E+L       +G E  
Sbjct: 304 EVTYLTSQESLNYNSDVRTAENCAEEIILSDSDS----------EELYTENYPLIGSEEF 363

Query: 362 S-------MGSSHEGHMPKTVVIDDGERSFNLRAC-------SSSNHAVTE---SCRDDV 421
           +       + +S+ G         D   S + R+        SSS   + E      D V
Sbjct: 364 TERVAPPILNASNSGKTTTNYRFSDCSASISPRSSEGTFEMQSSSTMGIAEFNMLDNDTV 423

Query: 422 KANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRR 481
             + +IL+MPP    + F + Y VVLILDDRE F     RSR+ ++N+ SQF++ +EI+ 
Sbjct: 424 CMDNSILAMPPRRSSKNFLEDYEVVLILDDRENF---GGRSRKTVDNIHSQFRVPVEIKH 483

Query: 482 LPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYL 541
           LPVGDGIWIAR + L  EYVLDFIVERKN+ DL  SI DNRY+DQKL+L +CGL+K+IYL
Sbjct: 484 LPVGDGIWIARDRKLHTEYVLDFIVERKNVADLCSSITDNRYKDQKLRLKKCGLRKLIYL 543

Query: 542 VEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDE 601
           VEGDPN  + +E IKTACFTTEILEGFDVQRT G A+T++ YG LT +IT+YY S     
Sbjct: 544 VEGDPNPLDTSERIKTACFTTEILEGFDVQRTPGYAETVRTYGNLTHSITEYY-STHFST 603

Query: 602 RLSHSDVCPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLAR 661
             + S VC  ++EF KKC +L K+TVSDVFALQLMQVPQVTEE A+AV+ LYPTL SLA+
Sbjct: 604 GANTSQVCLTYDEFTKKCDDLKKITVSDVFALQLMQVPQVTEEAALAVIGLYPTLFSLAK 660

Query: 662 AYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCG 675
           AYS+LDG+   QE+ML+ +S  LINAGASRNIF LVW  G
Sbjct: 664 AYSMLDGDTHAQEKMLKNKS-TLINAGASRNIFKLVWAEG 660

BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match: Q6BJ48 (Crossover junction endonuclease MUS81 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=MUS81 PE=3 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 8.1e-30
Identity = 173/732 (23.63%), Postives = 299/732 (40.85%), Query Frame = 0

Query: 21  WQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMKDLSQIKGVGK----WILKQV 80
           W +   + AT KG   K  +   KA  +V N    I   K L  ++ VG      + K++
Sbjct: 15  WLQEAAINATKKG--TKAAILYNKALGSVRNYPLPINDPKTLKSVQFVGDKTCIHLSKKL 74

Query: 81  QDFFKSGS---------------------EGSEPEDLA-GKGKRAKGTKRYVPQKNSVAY 140
           +++ K  +                     E S+  +L   K K+ +  K+Y+P+K S  Y
Sbjct: 75  EEYCKLNNFELPVAFGGLINGGVGEKRKHEVSDTSNLPDAKPKKQRKQKQYIPRKRSGGY 134

Query: 141 ALLVTLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVP 200
           A+L+ LY                                  + K+E+I  A        P
Sbjct: 135 AILLALY--------------------------FGDKKKTGLTKEEIIQRA-------TP 194

Query: 201 IGPEKGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAK-YMLTEEGQQVARECL 260
              +  K  P     S  ++YS WS +K+L +  LV  S   +K Y LTEEG ++A++  
Sbjct: 195 YSDKSFKSNP-----SANEFYSAWSSIKSLQTHDLVDSSGRSSKSYFLTEEGYELAKQ-- 254

Query: 261 MRSGLLDSTTNFDNAKGSSVLESCDLSDRNAVNLDSD-------VEVTSPPVGQSRQNKS 320
                L     F+++  ++ +   DLS  N V +  D        ++ S P+ +++ NK 
Sbjct: 255 -----LKDAEGFESSPITNHI--ADLSFDNQVRVTPDSSYSKISQQLDSSPLMKTKNNK- 314

Query: 321 TDVPLESLERF----MRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQ 380
                   +R+     R+  ++ ++L+        +P    S L P     LRE L    
Sbjct: 315 --------DRYGLGPSRLSSTRERILDL-----SSSPRLISSPLKPKDFV-LRESL---- 374

Query: 381 VEAVGRESRSMGSSHEGHMP--KTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNI 440
                       SSH    P      I   E   N +  +   H  ++   D    ++ +
Sbjct: 375 ------------SSHNSTRPLQDRESISKAEDGINTKTKARLVHDASKRIYDGTNYDIWV 434

Query: 441 LSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDG 500
                   GE     + ++LI+D+RE     S R R   +   +  K++ ++R L VGD 
Sbjct: 435 P-------GE-----FEIILIIDNRE---IRSQRDRDFFQTRLTSLKVECDVRPLSVGDV 494

Query: 501 IWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVE---- 560
           +W A+HK    E +L++I ERK +DDL  SI+D R+++QK +L + G+K+  YLVE    
Sbjct: 495 VWTAKHKKTGREVILNYICERKRLDDLVSSIKDGRFQEQKNRLKKSGMKQFYYLVEDVVT 554

Query: 561 GDPNS-SEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETD-- 620
            D N   + +++I+TA   T  +  F ++R   + DT+     LT+ I   +   +T+  
Sbjct: 555 SDMNKFGDMSDAIQTAMSMTMTISNFYLKRFKSIEDTIAFLASLTQVIKDQFAKNKTNLL 614

Query: 621 --------ERLSHSDVCPRFNEFIK------KCQEL-----------DKMTVSDVFALQL 674
                    +  +S +  +F E  +      +C  L             MTV + F L L
Sbjct: 615 VLKARSIKNQAEYSSLIAKFKEKFENRSTSYECAHLFSTFQDSMGKTGMMTVKETFILML 650

BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match: Q7SXA9 (Crossover junction endonuclease MUS81 OS=Danio rerio OX=7955 GN=mus81 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 5.2e-29
Identity = 158/592 (26.69%), Postives = 237/592 (40.03%), Query Frame = 0

Query: 95  KGKRAKGTKRYVPQKNSVAYALLVTLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNE 154
           K K AK  + YVPQK S  YA+L+TLYR+                               
Sbjct: 127 KEKGAKKKREYVPQKRSGGYAVLLTLYRH-----------------------MQMPGSKG 186

Query: 155 YMRKQELIDAAEASGLSRVPIGPEKGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSS 214
           +M + EL    EA  L       EK    P   GS     Y+ WS + TLI + LVVK+ 
Sbjct: 187 FMFRNEL--QTEAQPLC------EKSFTVP-DLGSK----YTAWSSVSTLIQKELVVKTH 246

Query: 215 CPAKYMLTEEGQQVARECLMRSGLLDSTTNFDNAKGSSVLESCDLSD-RNAVNLDSDVEV 274
            PA+Y LT++G  +A +              D+ +  +  E  D  D +N V+L      
Sbjct: 247 NPARYSLTDQGLSLAEK-------------LDSEETGTRHEDVDSQDGQNVVDL------ 306

Query: 275 TSPPVGQSRQNKSTDVPLESLERFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCR 334
                                                 +E  ED   +  SS  PAV   
Sbjct: 307 ------------------------------------TLEEEDEDEEKESWSSERPAVA-- 366

Query: 335 LREDLVYGQVEAVGRESRSMGSSHEGHMPKTVVIDDGERSFNLRACSSSNHAVTESCRDD 394
               L   Q   +  ES  MG S      +T +       ++L   S             
Sbjct: 367 ----LPVNQARGLMSESDPMGKSQTSETGRTAM------GWHLSPGS------------- 426

Query: 395 VKANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIR 454
                                 Y++VL +D  E     S R + +++ +  +  +  ++R
Sbjct: 427 ----------------------YDIVLCVDLCETTGGSSVRKQELVKEL-QRNSVTFDVR 486

Query: 455 RLPVGDGIWIARHK----------HLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKL 514
           +L VGD +W+AR +           +  E VLD+I+ERK +DDL  SI D R+R+QK +L
Sbjct: 487 KLNVGDFLWVARERVTPVPGQLRPPVGKELVLDYIIERKRMDDLCGSIIDGRFREQKFRL 546

Query: 515 LRCGLKKMIYLVE----GDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYL 574
            RCGL+K IYLVE       + S    +++ A   T++++GF V+R     ++      +
Sbjct: 547 KRCGLRKPIYLVEECGSAAAHLSIPESTLQQAIVNTQVVDGFFVKRVQDAKESAAYLTIM 573

Query: 575 TRAITQYYIS--------------QETDERLSHSDVCP--RFNEF----IK-KCQELDKM 634
           TR + + Y +              +   E++  +  C    F EF    IK KCQ     
Sbjct: 607 TRYLQKLYQNCTLLCRSRELEGDGEAESEKMVANLSCSLMAFTEFNYGAIKNKCQ----- 573

Query: 635 TVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEML 651
           TV +VFA QLMQ+  V+ + A AVL  Y T+ SL +AY       TE+E++L
Sbjct: 667 TVREVFARQLMQISGVSGDKAAAVLKHYSTVSSLLQAYDKCSSE-TEKEKLL 573

BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match: P87231 (Crossover junction endonuclease mus81 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mus81 PE=1 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 4.4e-28
Identity = 138/580 (23.79%), Postives = 233/580 (40.17%), Query Frame = 0

Query: 81  KSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTLYRYNGQIAISNVEFLFELIC 140
           KS SE S  +  + K    +  K YVP   S AY++L  LY                   
Sbjct: 94  KSSSETSSEKPRSVKKPTTRKRKVYVPSYRSGAYSILCALYML----------------- 153

Query: 141 NVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKGKGKPSQFGSSP--RDWYSGW 200
                     N +E+  K +++  A+                  S FGS+      Y+ W
Sbjct: 154 ----------NKHEFATKPQIVTMAQPY--------------CDSSFGSATDRNMRYTAW 213

Query: 201 SCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLDSTTNFDNAKGSSVLESCD 260
           S MKTLI++ LV ++  P+KY LT++G++V   C+  + + DS          SV +S  
Sbjct: 214 SAMKTLITKNLVYQTGHPSKYCLTDDGEEV---CIRLAKVDDSFQRKHTVSNFSVSKSD- 273

Query: 261 LSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRMGYSKHQVLNAFKEVSEDN 320
                    D D  +  PP   +  NK+        E  +      H       EVS+  
Sbjct: 274 ---------DHDSSLCQPPNFVTSINKAGSSSDHGGELHVTYCPVDH------NEVSDG- 333

Query: 321 PYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGHMPKTVVIDDGERSFNLRA 380
                          +  D+   QV+       S+   H+ H      I + E+  +L  
Sbjct: 334 ---------------VETDIDVDQVD-------SLTGIHDHH------IINNEQLIDL-- 393

Query: 381 CSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRM 440
                    +  +   ++N++ L +  + F         V L++D RE     S   R +
Sbjct: 394 -------TEQEKKQPNESNLSNLKIETVLFSN-----CTVFLLIDTRE---IRSPLDRNL 453

Query: 441 -IENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYR 500
            I+ + + F +  ++R L +GD +W+AR      E VLDF+VERK  DDL  SI+D R+ 
Sbjct: 454 IIDKLTNDFGVNCQVRSLELGDALWVARDMESGQEVVLDFVVERKRYDDLVASIKDGRFH 513

Query: 501 DQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYG 560
           +QK +L + G++ + Y++E         ESI+TA   T++ + F V+ T  L  ++    
Sbjct: 514 EQKARLKKSGIRSVTYILEESSYDESFTESIRTAVSNTQVDQLFHVRHTRSLEHSVSLLA 567

Query: 561 YLTRAITQYYISQET------------------DERLSHSDVCP---RFNEFIKKCQELD 620
            +T+ I  +Y  ++T                  ++ L      P    ++ F     +  
Sbjct: 574 EMTKQINLFYEKRKTLAVIPDLSIEAKTYESLREQLLKIDPSTPYHISYHAFSSVLSKSS 567

Query: 621 KMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAY 637
            +TV D+F   LM +  ++   A+ +   YPT + L  AY
Sbjct: 634 TLTVGDIFIRMLMTIKGISASKAIEIQKKYPTFMHLFEAY 567

BLAST of Spg009758 vs. ExPASy TrEMBL
Match: A0A6J1GYZ8 (crossover junction endonuclease MUS81 OS=Cucurbita moschata OX=3662 GN=LOC111458164 PE=3 SV=1)

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 576/675 (85.33%), Postives = 611/675 (90.52%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQVQ  FK+GSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61  DLSQIKGVGKWILKQVQVIFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKP QFGSS RDWYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           S  NFD  KGSS+LESC +SDR+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCGISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
           GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+RS GSS+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTGSSNEGH 360

Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
           +PK VV D+GERSFNLRACSSSNH++TES +D VK N NILSMPPLSFGEKFED Y+VVL
Sbjct: 361 VPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSVVL 420

Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
           ILDDREQFANHSSRSRRMIEN+CSQFKI+IEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIEIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480

Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
           RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540

Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
           FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DERLSH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTV 600

Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
           SDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINA 650

Query: 661 GASRNIFHLVWGCGK 676
            ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 650

BLAST of Spg009758 vs. ExPASy TrEMBL
Match: A0A6J1JV85 (crossover junction endonuclease MUS81 OS=Cucurbita maxima OX=3661 GN=LOC111488639 PE=3 SV=1)

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 574/675 (85.04%), Postives = 608/675 (90.07%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKG SDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGTSDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQVQ FFKSGSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61  DLSQIKGVGKWILKQVQVFFKSGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKP QFGSS R+WYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTREWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           S  NFD  KGSS+LESCD+S R+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCDISGRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
           GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+R  GSS+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRLTGSSNEGH 360

Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
           +PK V+ D+GERSFNLRACSSSN ++TESC+D VK NMNILSMPPLSFGEKFED Y+VVL
Sbjct: 361 VPKIVISDNGERSFNLRACSSSNQSITESCKDVVKENMNILSMPPLSFGEKFEDAYSVVL 420

Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
           ILDDREQFANHSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480

Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
           RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540

Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
           FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DER SH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERPSHFDVCPRLDAFIKKCQELDKMTV 600

Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
           SDVFALQLMQVPQVTEE+AV VLDLYPTLLSLARAYSLLD NVT QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVTVLDLYPTLLSLARAYSLLDDNVTHQEEMLRRQSNNLINA 650

Query: 661 GASRNIFHLVWGCGK 676
            ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 650

BLAST of Spg009758 vs. ExPASy TrEMBL
Match: A0A1S3BFN6 (crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489485 PE=3 SV=1)

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 575/675 (85.19%), Postives = 610/675 (90.37%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MDRQR PV CPENE+LAAFMWQKRLEMAATPKGISDKVDMTLYKAYFN+CNSK SI+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQVQDFFKSGSEGS PE+LAGKGK+ KGT+RYVPQKNSVAYALL+TL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKS+CPAKYMLTEEGQQVARECLMRSGLL 
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           S+ NFDN KGSS+LESCD+S+ +A  LDSDVEVTSP V Q  +NK+ DVPLESLERFMRM
Sbjct: 241 SSKNFDNIKGSSILESCDISNHSANYLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
           GYSKHQV+N+FKEVS+DNP KD SSLWPAVLCRLREDLVYGQVEAVGRE+RSMG SHEG 
Sbjct: 301 GYSKHQVINSFKEVSKDNPNKDTSSLWPAVLCRLREDLVYGQVEAVGRENRSMG-SHEGS 360

Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
            PK VV D+GERSFN RACSSSN ++ ES RD VK + NILSMPPLSFGEKFED YNVVL
Sbjct: 361 EPKNVVNDNGERSFNSRACSSSNPSIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVVL 420

Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
           ILDDREQFA HSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIVE
Sbjct: 421 ILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIVE 480

Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
           RK+IDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKDIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540

Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
           FDVQRTSGL+DTLKKYGYLT AITQYYI Q +D+R+SHSDVCPR ++FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTV 600

Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
           SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 649

Query: 661 GASRNIFHLVWGCGK 676
           GASRNIFHLVWG G+
Sbjct: 661 GASRNIFHLVWGSGR 649

BLAST of Spg009758 vs. ExPASy TrEMBL
Match: A0A0A0KWV2 (ERCC4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623550 PE=3 SV=1)

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 571/676 (84.47%), Postives = 608/676 (89.94%), Query Frame = 0

Query: 1   MDRQRLPVV-CPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSM 60
           MDRQR P V CPENE+LA FMWQKRLEMAATPKGISDKVDMTLYKAY NVCNSK SI SM
Sbjct: 1   MDRQRSPAVFCPENEDLAEFMWQKRLEMAATPKGISDKVDMTLYKAYLNVCNSKVSITSM 60

Query: 61  KDLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVT 120
           KDLSQIKGVGKWILKQVQDFFKSGS+GSEPE+LAGKGK+ KGT+RYVPQKNSVAYALL+T
Sbjct: 61  KDLSQIKGVGKWILKQVQDFFKSGSDGSEPEELAGKGKKTKGTRRYVPQKNSVAYALLIT 120

Query: 121 LYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEK 180
           LY                         R T+NGNEYMRKQELIDAAEASGLSRV I PEK
Sbjct: 121 LY-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEK 180

Query: 181 GKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL 240
           GKGKPSQFGSS RDWYSGWSCMKTLISRGLVVKS+CPAKYMLTEEGQQVARECLMRSGLL
Sbjct: 181 GKGKPSQFGSSSRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLL 240

Query: 241 DSTTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMR 300
            S+ NFDN KGSS+LESCD+S+ +A  LDSDVEVTSP V QS ++KS DVPLESLERFMR
Sbjct: 241 SSSKNFDNTKGSSILESCDISNHSANYLDSDVEVTSPSVSQSTKHKSIDVPLESLERFMR 300

Query: 301 MGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEG 360
           MGYSKHQV+N+FKEVS+DNP KDISSLWPAVLCRLREDLVYGQVEA+GRE+RSMGSS EG
Sbjct: 301 MGYSKHQVINSFKEVSKDNPNKDISSLWPAVLCRLREDLVYGQVEAIGRENRSMGSSLEG 360

Query: 361 HMPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVV 420
             PK VV  +GERSF+ RACSSSN ++ ES RD VK N NILSMPPLSFGEKFED YNVV
Sbjct: 361 SEPKNVVNVNGERSFSSRACSSSNPSIIESRRDSVKGNTNILSMPPLSFGEKFEDCYNVV 420

Query: 421 LILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFA HSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIW+ARHKHLDNEYVLDFIV
Sbjct: 421 LILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWVARHKHLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERK+++DLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKDVEDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMT 600
           GFDVQRTSGL+DTLKKYGYLT AITQYYI Q +D+R+SHSDVCPR ++FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLIN 660
           VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSL RAYSLLDGNVTEQEEMLRR+SNNLIN
Sbjct: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLVRAYSLLDGNVTEQEEMLRRRSNNLIN 651

Query: 661 AGASRNIFHLVWGCGK 676
           AGASRNIFHLVWGCGK
Sbjct: 661 AGASRNIFHLVWGCGK 651

BLAST of Spg009758 vs. ExPASy TrEMBL
Match: A0A6J1CEX9 (crossover junction endonuclease MUS81 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010103 PE=3 SV=1)

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 573/676 (84.76%), Postives = 606/676 (89.64%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           M+RQRLPVVCPENE+LA FMWQKRLEMAATPKGIS++V+MTLYKAYFNVCNSKASIKSMK
Sbjct: 1   MERQRLPVVCPENEDLAGFMWQKRLEMAATPKGISERVEMTLYKAYFNVCNSKASIKSMK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
           DLSQIKGVGKWILKQVQDFFKSGSE SEP DLAGKGK+ KGTKRYVPQKNSVAY LL+TL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEDSEPADLAGKGKKKKGTKRYVPQKNSVAYTLLITL 120

Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
           Y                         R T+NGNEYMRKQELIDAAEASGLSRVPI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVPIAPEKG 180

Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
           KGKP+QFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYM TEEG+QVARECL RSGL +
Sbjct: 181 KGKPAQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMPTEEGKQVARECLERSGLSN 240

Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
           ST NFDN KGSS+L+SCD+SD +   LDS+VEVTSP  G SRQNKS +VP ESL+RFMRM
Sbjct: 241 STMNFDNDKGSSILKSCDISDHSVDCLDSEVEVTSPCAGVSRQNKSINVPPESLQRFMRM 300

Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMG-SSHEG 360
           GYSK+QVL+AFKEVSEDNP KDISSLWPAVLCRLRED VYGQVEAVGRE+R +G SS+EG
Sbjct: 301 GYSKNQVLDAFKEVSEDNPNKDISSLWPAVLCRLREDHVYGQVEAVGRENRFLGSSSYEG 360

Query: 361 HMPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVV 420
           HM KT VID+ ERSF LR+CSSS+HA+TES RD VKANMNILSMPPLSFGEKFED Y VV
Sbjct: 361 HMRKTAVIDNVERSFTLRSCSSSDHAITESSRDCVKANMNILSMPPLSFGEKFEDAYKVV 420

Query: 421 LILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQF N SSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV
Sbjct: 421 LILDDREQFVNQSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERKNIDDLRCSIRDNRY+DQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKNIDDLRCSIRDNRYKDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMT 600
           GFDVQRTSGL+DTLKKYGYLT AIT YYISQ + ERLS SDVCP  NEFIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITHYYISQASSERLSRSDVCPPLNEFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLIN 660
           VSDVFALQLMQVPQVTEEVAVAV+DLYPTLL LARAYSLLDGNV+EQEEMLRRQS NLIN
Sbjct: 601 VSDVFALQLMQVPQVTEEVAVAVVDLYPTLLLLARAYSLLDGNVSEQEEMLRRQSKNLIN 651

Query: 661 AGASRNIFHLVWGCGK 676
           AGASRNIFHLVWG GK
Sbjct: 661 AGASRNIFHLVWGSGK 651

BLAST of Spg009758 vs. TAIR 10
Match: AT4G30870.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 674.9 bits (1740), Expect = 6.9e-194
Identity = 366/694 (52.74%), Postives = 472/694 (68.01%), Query Frame = 0

Query: 1   MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
           MD +R  V+CPEN  LAA++ QK+ E A  PKG+S+ ++ T  K Y +VC++K  I ++K
Sbjct: 1   MDDER-RVLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLK 60

Query: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGK--GKRAKGTKRYVPQKNSVAYALLV 120
           DLSQIKG GKW++K ++ +F +  E SE EDL     GK+A G KRY+PQ+NSV YALL+
Sbjct: 61  DLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLI 120

Query: 121 TLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPE 180
           TL+                         R T+NG E+MRKQELIDAA+A+GLS  P+GPE
Sbjct: 121 TLH-------------------------RRTTNGKEFMRKQELIDAADANGLSHAPVGPE 180

Query: 181 KGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGL 240
           KGKGK +  G S R+WYSGWSCM TLI +GLVVKSS PAKYMLT EG++VA EC++RSGL
Sbjct: 181 KGKGK-AGLGHSKREWYSGWSCMTTLIQKGLVVKSSNPAKYMLTVEGREVANECILRSGL 240

Query: 241 LDSTTNFDNAKGSSVLESCDLSDRN---AVNLDSDVEVTSPPVGQSRQNKSTDVPLESLE 300
            DS       +     ++    ++N   +  +  ++    P     R    + +P + LE
Sbjct: 241 PDSVDILSVDEMDPTPQAKKTPNQNPTCSFTMREELPYVDP-----RCRAQSAIPSDILE 300

Query: 301 RFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGS 360
           +F   GYSK QV+ AF+EVS+ +  KD S+LW +V+C LR+  VY        +SR+   
Sbjct: 301 KFTPFGYSKEQVVAAFREVSDGSGDKDPSTLWLSVMCHLRQAEVYNSCP----DSRNSKK 360

Query: 361 SHEGHMPKTV--VIDDGERSFNLRAC----------SSSNHAV----TESCRDDVKANMN 420
              G     +  V  +G R+   R+C          S S+HAV    +    D  K   N
Sbjct: 361 DSSGPFKSQIRQVDLEGSRAKKFRSCNDGSTLNPCSSGSSHAVKACSSSLASDGTKGITN 420

Query: 421 ILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGD 480
           I  +PPL FGE FE+ Y+V+LILDDRE+FA   SRSR ++EN+CS+F I+IE+RRLPVGD
Sbjct: 421 IPRLPPLQFGETFEEAYDVILILDDREKFATKGSRSRNIVENICSEFNIKIEVRRLPVGD 480

Query: 481 GIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDP 540
            IWIARHK+L+ EYVLDFI ERKN+DD+R SIRDNRYRDQKL+L R G KK+IY++EGDP
Sbjct: 481 CIWIARHKYLETEYVLDFIAERKNVDDMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDP 540

Query: 541 NSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHS 600
           N S+AAESIKTACFTTEILEGFDV RT GL +TL+KYGYLT++I QYY  +  D   S  
Sbjct: 541 NHSDAAESIKTACFTTEILEGFDVLRTHGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKG 600

Query: 601 DV-CPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSL 660
              CP F+ F+K+CQ+LDKMT+SDVFA+QLMQVPQVTEE+A+AVLD+YPTLLSLA AYS 
Sbjct: 601 AASCPSFDSFVKRCQDLDKMTISDVFAIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSH 658

Query: 661 LDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWG 673
           L+ +V+ QEEMLR +SNN+I A AS+NIF LVWG
Sbjct: 661 LEADVSAQEEMLRNRSNNVICASASKNIFKLVWG 658

BLAST of Spg009758 vs. TAIR 10
Match: AT5G39770.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 439.5 bits (1129), Expect = 4.9e-123
Identity = 280/651 (43.01%), Postives = 367/651 (56.37%), Query Frame = 0

Query: 31   PKGISDKVDMTLYKAYFNVCNSKASIKSMKDLSQIKGVGKWILKQVQDFFKSGSEGSEPE 90
            PKG+S+  +M   KAY NVC++K SI ++KDLS+IKG GK+++K +Q +F +G+  SE E
Sbjct: 762  PKGLSEYQEMKFVKAYRNVCDAKDSIDTLKDLSKIKGFGKYMVKLMQGYFVTGAASSEQE 821

Query: 91   DLAGK--GKRAKGTKRYVPQKNSVAYALLVTLYRYNGQIAISNVEFLFELICNVRFIIRS 150
             L G   GK+AKG++ Y PQ+N+ AYALL TL+R+                         
Sbjct: 822  ALPGNRAGKKAKGSEPYKPQRNTAAYALL-TLHRF------------------------K 881

Query: 151  TSNGNEYMRKQELIDAAEASGLSRVPIGPEKGKGKPSQFGSSPRDWYSGWSCMKTLISRG 210
            T+NG + MRKQ+L+DAA+ASGLS V I PEKGKGK +  G+S R+WYSGWSCM  LI +G
Sbjct: 882  TANGKKLMRKQKLMDAADASGLSHVSIAPEKGKGK-AGLGTSKREWYSGWSCMSKLIEKG 941

Query: 211  LVVKSSCPAKYMLTEEGQQVARECLMRSGLLDSTTNFDNAKGSSVLESCDLSDRNAVNLD 270
            LVVK S PAKYMLT EG+ VA +   RS            +G S                
Sbjct: 942  LVVKFSNPAKYMLTVEGRNVADDLDPRS------------RGQS---------------- 1001

Query: 271  SDVEVTSPPVGQSRQNKSTDVPLESLERFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWP 330
                + + P  ++++  +TD                                        
Sbjct: 1002 ---SIPAGPSSRAQEFLATDT--------------------------------------- 1061

Query: 331  AVLCRLREDLVYGQVEAVGRESRSMGSSHEGHMPKTVVIDDGERSFNLRACSSSNHAVTE 390
                        GQV+  G  ++   SS     P +  I     S  L+ACSSS      
Sbjct: 1062 ------------GQVDLEGSRAKKFRSS----SPCSSSI-----SHALKACSSS------ 1121

Query: 391  SCRDDVKANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKI 450
               D  +    I  +PPL FGE FE++Y+V+LILDDREQFA                   
Sbjct: 1122 LASDGTEGVTTIPRLPPLRFGEAFEEVYDVILILDDREQFATKG---------------- 1181

Query: 451  QIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGL 510
                             HK+  +EYVLDFIVERKN+DD+R SI DNRY+DQKL+L R G+
Sbjct: 1182 -----------------HKYQQDEYVLDFIVERKNVDDMRSSIMDNRYKDQKLRLQRSGI 1241

Query: 511  KKMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYI 570
            KK+IY++EGDPN S+ AESIKTACFTTEILEGFDV RT+GL +TL+KYGYLT++I QYY 
Sbjct: 1242 KKLIYILEGDPNQSKTAESIKTACFTTEILEGFDVVRTNGLGETLRKYGYLTKSIYQYYK 1255

Query: 571  SQETDERLSHSDVCPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEE---------VA 630
            S   D++   +D CP +++F+++C +L KMT+SDVFA+QLMQV  + +           A
Sbjct: 1302 SLVNDDQRKVADSCPSYDDFVQRCPDLGKMTISDVFAIQLMQVLSLAKPSFGFLSATFFA 1255

Query: 631  VAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLV 671
            +AVLD Y TL SLA AYS LD NV+ QEEMLR QSNN+I A AS+NI+ LV
Sbjct: 1362 IAVLDKYKTLRSLASAYSKLD-NVSAQEEMLRNQSNNVICASASKNIYKLV 1255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892703.10.0e+0086.58crossover junction endonuclease MUS81 isoform X1 [Benincasa hispida][more]
XP_022956424.10.0e+0085.33crossover junction endonuclease MUS81 [Cucurbita moschata][more]
XP_023514312.10.0e+0085.48crossover junction endonuclease MUS81 [Cucurbita pepo subsp. pepo][more]
XP_022992270.10.0e+0085.04crossover junction endonuclease MUS81 [Cucurbita maxima][more]
XP_008446914.10.0e+0085.19PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo] >XP_0... [more]
Match NameE-valueIdentityDescription
Q5W9E79.7e-19352.74Crossover junction endonuclease MUS81 OS=Arabidopsis thaliana OX=3702 GN=MUS81 P... [more]
Q8GT062.9e-15246.86Crossover junction endonuclease MUS81 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6BJ488.1e-3023.63Crossover junction endonuclease MUS81 OS=Debaryomyces hansenii (strain ATCC 3623... [more]
Q7SXA95.2e-2926.69Crossover junction endonuclease MUS81 OS=Danio rerio OX=7955 GN=mus81 PE=1 SV=1[more]
P872314.4e-2823.79Crossover junction endonuclease mus81 OS=Schizosaccharomyces pombe (strain 972 /... [more]
Match NameE-valueIdentityDescription
A0A6J1GYZ80.0e+0085.33crossover junction endonuclease MUS81 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
A0A6J1JV850.0e+0085.04crossover junction endonuclease MUS81 OS=Cucurbita maxima OX=3661 GN=LOC11148863... [more]
A0A1S3BFN60.0e+0085.19crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A0A0KWV20.0e+0084.47ERCC4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623550 PE=3 ... [more]
A0A6J1CEX90.0e+0084.76crossover junction endonuclease MUS81 isoform X1 OS=Momordica charantia OX=3673 ... [more]
Match NameE-valueIdentityDescription
AT4G30870.16.9e-19452.74Restriction endonuclease, type II-like superfamily protein [more]
AT5G39770.14.9e-12343.01Restriction endonuclease, type II-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006166ERCC4 domainSMARTSM00891ERCC4_2coord: 419..519
e-value: 8.8E-21
score: 85.0
IPR006166ERCC4 domainPFAMPF02732ERCC4coord: 423..553
e-value: 7.7E-19
score: 68.4
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 97..244
e-value: 2.2E-29
score: 103.4
NoneNo IPR availableGENE3D3.40.50.10130coord: 413..563
e-value: 1.2E-26
score: 95.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..191
IPR042530EME1/EME2, C-terminal domainGENE3D1.10.150.670coord: 598..673
e-value: 5.2E-12
score: 48.1
IPR033309Crossover junction endonuclease Mus81PANTHERPTHR13451CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81coord: 9..672
IPR011335Restriction endonuclease type II-likeSUPERFAMILY52980Restriction endonuclease-likecoord: 416..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009758.1Spg009758.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051301 cell division
biological_process GO:0000737 DNA catabolic process, endonucleolytic
biological_process GO:0000727 double-strand break repair via break-induced replication
biological_process GO:0031573 mitotic intra-S DNA damage checkpoint signaling
biological_process GO:0000712 resolution of meiotic recombination intermediates
biological_process GO:0006302 double-strand break repair
cellular_component GO:0048476 Holliday junction resolvase complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0048257 3'-flap endonuclease activity
molecular_function GO:0008821 crossover junction endodeoxyribonuclease activity
molecular_function GO:0003677 DNA binding
molecular_function GO:0004518 nuclease activity