Homology
BLAST of Spg009758 vs. NCBI nr
Match:
XP_038892703.1 (crossover junction endonuclease MUS81 isoform X1 [Benincasa hispida])
HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 587/678 (86.58%), Postives = 615/678 (90.71%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MDRQR PVVCPENE++A FMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSK IKSMK
Sbjct: 1 MDRQRSPVVCPENEDIATFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKVPIKSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQV DFFK+GSEGS PE+L GKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61 DLSQIKGVGKWILKQVHDFFKNGSEGSGPEELVGKGKKTKGTKRYVPQKNSVAYALLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDA+EASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDASEASGLSRVAIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKS+CPAKYMLTEEGQQVARECLMRSGLL
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
ST N DN KGSS+LESCD+SD +A +LDSDVEVTSP +S QNKSTDVPLESLERFMRM
Sbjct: 241 STKNVDNTKGSSILESCDISDHSANSLDSDVEVTSPTAIKSSQNKSTDVPLESLERFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESR--SMGSSHE 360
GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+R S SSH+
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRLTSSSSSHD 360
Query: 361 GHMPKTVVIDDGERSFNLRACSSSNHAV-TESCRDDVKANMNILSMPPLSFGEKFEDLYN 420
+ PK VV D+GER FNLRACSSSN ++ +ESCRD VKANMNILSMPPLSFGEKFED YN
Sbjct: 361 RNGPKIVVNDNGERFFNLRACSSSNPSISSESCRDGVKANMNILSMPPLSFGEKFEDSYN 420
Query: 421 VVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF 480
VVLILDDREQFANHSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF
Sbjct: 421 VVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF 480
Query: 481 IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEI 540
IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEI
Sbjct: 481 IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEI 540
Query: 541 LEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDK 600
LEGFDVQRTSGL+DTLKKYGYLTRAITQYYI Q +DERLSHSDVCPR NEFIKKCQELDK
Sbjct: 541 LEGFDVQRTSGLSDTLKKYGYLTRAITQYYILQASDERLSHSDVCPRLNEFIKKCQELDK 600
Query: 601 MTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNL 660
MTVSDVFALQLMQVPQVTEEVAVAVL+LYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNL
Sbjct: 601 MTVSDVFALQLMQVPQVTEEVAVAVLELYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNL 653
Query: 661 INAGASRNIFHLVWGCGK 676
INAGASRNIFHLVWGCGK
Sbjct: 661 INAGASRNIFHLVWGCGK 653
BLAST of Spg009758 vs. NCBI nr
Match:
XP_022956424.1 (crossover junction endonuclease MUS81 [Cucurbita moschata])
HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 576/675 (85.33%), Postives = 611/675 (90.52%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1 MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQVQ FK+GSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61 DLSQIKGVGKWILKQVQVIFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKP QFGSS RDWYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
S NFD KGSS+LESC +SDR+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCGISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+RS GSS+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTGSSNEGH 360
Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
+PK VV D+GERSFNLRACSSSNH++TES +D VK N NILSMPPLSFGEKFED Y+VVL
Sbjct: 361 VPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSVVL 420
Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
ILDDREQFANHSSRSRRMIEN+CSQFKI+IEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIEIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DERLSH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTV 600
Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
SDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINA 650
Query: 661 GASRNIFHLVWGCGK 676
ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 650
BLAST of Spg009758 vs. NCBI nr
Match:
XP_023514312.1 (crossover junction endonuclease MUS81 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 577/675 (85.48%), Postives = 612/675 (90.67%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1 MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQVQ FFK+GSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61 DLSQIKGVGKWILKQVQVFFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKP QFGSS RDWYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
S NFD KGSS+LESCD+SDR+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCDISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+RS G S+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTG-SNEGH 360
Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
+PK VV D+GERSFNLRACSSSNH++TES +D VK N NILSMPPLSFGEKFED Y++VL
Sbjct: 361 VPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSIVL 420
Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
ILDDREQFANHSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DERLSH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTV 600
Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
SDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINA 649
Query: 661 GASRNIFHLVWGCGK 676
ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 649
BLAST of Spg009758 vs. NCBI nr
Match:
XP_022992270.1 (crossover junction endonuclease MUS81 [Cucurbita maxima])
HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 574/675 (85.04%), Postives = 608/675 (90.07%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKG SDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1 MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGTSDKVDMTLYKAYFNVCNSKDSIKSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQVQ FFKSGSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61 DLSQIKGVGKWILKQVQVFFKSGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKP QFGSS R+WYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTREWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
S NFD KGSS+LESCD+S R+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCDISGRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+R GSS+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRLTGSSNEGH 360
Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
+PK V+ D+GERSFNLRACSSSN ++TESC+D VK NMNILSMPPLSFGEKFED Y+VVL
Sbjct: 361 VPKIVISDNGERSFNLRACSSSNQSITESCKDVVKENMNILSMPPLSFGEKFEDAYSVVL 420
Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
ILDDREQFANHSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DER SH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERPSHFDVCPRLDAFIKKCQELDKMTV 600
Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
SDVFALQLMQVPQVTEE+AV VLDLYPTLLSLARAYSLLD NVT QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVTVLDLYPTLLSLARAYSLLDDNVTHQEEMLRRQSNNLINA 650
Query: 661 GASRNIFHLVWGCGK 676
ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 650
BLAST of Spg009758 vs. NCBI nr
Match:
XP_008446914.1 (PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo] >XP_008446915.1 PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo])
HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 575/675 (85.19%), Postives = 610/675 (90.37%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MDRQR PV CPENE+LAAFMWQKRLEMAATPKGISDKVDMTLYKAYFN+CNSK SI+SMK
Sbjct: 1 MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQVQDFFKSGSEGS PE+LAGKGK+ KGT+RYVPQKNSVAYALL+TL
Sbjct: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKS+CPAKYMLTEEGQQVARECLMRSGLL
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
S+ NFDN KGSS+LESCD+S+ +A LDSDVEVTSP V Q +NK+ DVPLESLERFMRM
Sbjct: 241 SSKNFDNIKGSSILESCDISNHSANYLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
GYSKHQV+N+FKEVS+DNP KD SSLWPAVLCRLREDLVYGQVEAVGRE+RSMG SHEG
Sbjct: 301 GYSKHQVINSFKEVSKDNPNKDTSSLWPAVLCRLREDLVYGQVEAVGRENRSMG-SHEGS 360
Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
PK VV D+GERSFN RACSSSN ++ ES RD VK + NILSMPPLSFGEKFED YNVVL
Sbjct: 361 EPKNVVNDNGERSFNSRACSSSNPSIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVVL 420
Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
ILDDREQFA HSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIVE
Sbjct: 421 ILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIVE 480
Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
RK+IDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKDIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
FDVQRTSGL+DTLKKYGYLT AITQYYI Q +D+R+SHSDVCPR ++FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTV 600
Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 649
Query: 661 GASRNIFHLVWGCGK 676
GASRNIFHLVWG G+
Sbjct: 661 GASRNIFHLVWGSGR 649
BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match:
Q5W9E7 (Crossover junction endonuclease MUS81 OS=Arabidopsis thaliana OX=3702 GN=MUS81 PE=1 SV=1)
HSP 1 Score: 674.9 bits (1740), Expect = 9.7e-193
Identity = 366/694 (52.74%), Postives = 472/694 (68.01%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MD +R V+CPEN LAA++ QK+ E A PKG+S+ ++ T K Y +VC++K I ++K
Sbjct: 1 MDDER-RVLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGK--GKRAKGTKRYVPQKNSVAYALLV 120
DLSQIKG GKW++K ++ +F + E SE EDL GK+A G KRY+PQ+NSV YALL+
Sbjct: 61 DLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLI 120
Query: 121 TLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPE 180
TL+ R T+NG E+MRKQELIDAA+A+GLS P+GPE
Sbjct: 121 TLH-------------------------RRTTNGKEFMRKQELIDAADANGLSHAPVGPE 180
Query: 181 KGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGL 240
KGKGK + G S R+WYSGWSCM TLI +GLVVKSS PAKYMLT EG++VA EC++RSGL
Sbjct: 181 KGKGK-AGLGHSKREWYSGWSCMTTLIQKGLVVKSSNPAKYMLTVEGREVANECILRSGL 240
Query: 241 LDSTTNFDNAKGSSVLESCDLSDRN---AVNLDSDVEVTSPPVGQSRQNKSTDVPLESLE 300
DS + ++ ++N + + ++ P R + +P + LE
Sbjct: 241 PDSVDILSVDEMDPTPQAKKTPNQNPTCSFTMREELPYVDP-----RCRAQSAIPSDILE 300
Query: 301 RFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGS 360
+F GYSK QV+ AF+EVS+ + KD S+LW +V+C LR+ VY +SR+
Sbjct: 301 KFTPFGYSKEQVVAAFREVSDGSGDKDPSTLWLSVMCHLRQAEVYNSCP----DSRNSKK 360
Query: 361 SHEGHMPKTV--VIDDGERSFNLRAC----------SSSNHAV----TESCRDDVKANMN 420
G + V +G R+ R+C S S+HAV + D K N
Sbjct: 361 DSSGPFKSQIRQVDLEGSRAKKFRSCNDGSTLNPCSSGSSHAVKACSSSLASDGTKGITN 420
Query: 421 ILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGD 480
I +PPL FGE FE+ Y+V+LILDDRE+FA SRSR ++EN+CS+F I+IE+RRLPVGD
Sbjct: 421 IPRLPPLQFGETFEEAYDVILILDDREKFATKGSRSRNIVENICSEFNIKIEVRRLPVGD 480
Query: 481 GIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDP 540
IWIARHK+L+ EYVLDFI ERKN+DD+R SIRDNRYRDQKL+L R G KK+IY++EGDP
Sbjct: 481 CIWIARHKYLETEYVLDFIAERKNVDDMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDP 540
Query: 541 NSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHS 600
N S+AAESIKTACFTTEILEGFDV RT GL +TL+KYGYLT++I QYY + D S
Sbjct: 541 NHSDAAESIKTACFTTEILEGFDVLRTHGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKG 600
Query: 601 DV-CPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSL 660
CP F+ F+K+CQ+LDKMT+SDVFA+QLMQVPQVTEE+A+AVLD+YPTLLSLA AYS
Sbjct: 601 AASCPSFDSFVKRCQDLDKMTISDVFAIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSH 658
Query: 661 LDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWG 673
L+ +V+ QEEMLR +SNN+I A AS+NIF LVWG
Sbjct: 661 LEADVSAQEEMLRNRSNNVICASASKNIFKLVWG 658
BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match:
Q8GT06 (Crossover junction endonuclease MUS81 OS=Oryza sativa subsp. japonica OX=39947 GN=MUS81 PE=1 SV=1)
HSP 1 Score: 540.4 bits (1391), Expect = 2.9e-152
Identity = 328/700 (46.86%), Postives = 428/700 (61.14%), Query Frame = 0
Query: 2 DRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMKD 61
+ ++L V ENE +A + +K M P G+ + + TLYK+Y NVC +K I+S+KD
Sbjct: 4 EARQLKVHLRENEAVAQCVLEKWRSMEEKPGGLKENLAHTLYKSYRNVCAAKEPIRSLKD 63
Query: 62 LSQIKGVGKWILKQVQDFF-KSGSEGSEPEDLAG--KGKRAKGTKRYVPQKNSVAYALLV 121
L QIKGVGKW+++Q++ F +S + S PE A KGK+A G+KRYVPQKNS AYA+L+
Sbjct: 64 LYQIKGVGKWVIRQLKGSFPESSPDLSPPESNAAGEKGKKAGGSKRYVPQKNSAAYAILI 123
Query: 122 TLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPE 181
TL+ R T NG +M+KQELIDA EASGLSR IGP+
Sbjct: 124 TLH-------------------------RETINGKSHMKKQELIDATEASGLSRSAIGPD 183
Query: 182 KGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGL 241
K K KP F SS +DWY+GWSCMKTL S+GLV KS PAKYM+TEEG+ A ECL RSGL
Sbjct: 184 KSKAKPGAFASSQKDWYTGWSCMKTLTSKGLVAKSGNPAKYMITEEGKSTALECLSRSGL 243
Query: 242 LDSTTNFDNAKGSSVLESCDLSDR-NAVNLDSDVEVTSPP---VGQ---SRQNKSTDVPL 301
D +S ++ + S + N + + S VE +S P +G+ S N +T
Sbjct: 244 DDHAAPL---VINSAPDTSNASHKLNNICMTSFVETSSGPSRAIGRPKTSIANPATKTSP 303
Query: 302 ESLERFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESR 361
E + + + + + +E+ D S E+L +G E
Sbjct: 304 EVTYLTSQESLNYNSDVRTAENCAEEIILSDSDS----------EELYTENYPLIGSEEF 363
Query: 362 S-------MGSSHEGHMPKTVVIDDGERSFNLRAC-------SSSNHAVTE---SCRDDV 421
+ + +S+ G D S + R+ SSS + E D V
Sbjct: 364 TERVAPPILNASNSGKTTTNYRFSDCSASISPRSSEGTFEMQSSSTMGIAEFNMLDNDTV 423
Query: 422 KANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRR 481
+ +IL+MPP + F + Y VVLILDDRE F RSR+ ++N+ SQF++ +EI+
Sbjct: 424 CMDNSILAMPPRRSSKNFLEDYEVVLILDDRENF---GGRSRKTVDNIHSQFRVPVEIKH 483
Query: 482 LPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYL 541
LPVGDGIWIAR + L EYVLDFIVERKN+ DL SI DNRY+DQKL+L +CGL+K+IYL
Sbjct: 484 LPVGDGIWIARDRKLHTEYVLDFIVERKNVADLCSSITDNRYKDQKLRLKKCGLRKLIYL 543
Query: 542 VEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDE 601
VEGDPN + +E IKTACFTTEILEGFDVQRT G A+T++ YG LT +IT+YY S
Sbjct: 544 VEGDPNPLDTSERIKTACFTTEILEGFDVQRTPGYAETVRTYGNLTHSITEYY-STHFST 603
Query: 602 RLSHSDVCPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLAR 661
+ S VC ++EF KKC +L K+TVSDVFALQLMQVPQVTEE A+AV+ LYPTL SLA+
Sbjct: 604 GANTSQVCLTYDEFTKKCDDLKKITVSDVFALQLMQVPQVTEEAALAVIGLYPTLFSLAK 660
Query: 662 AYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWGCG 675
AYS+LDG+ QE+ML+ +S LINAGASRNIF LVW G
Sbjct: 664 AYSMLDGDTHAQEKMLKNKS-TLINAGASRNIFKLVWAEG 660
BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match:
Q6BJ48 (Crossover junction endonuclease MUS81 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=MUS81 PE=3 SV=2)
HSP 1 Score: 133.7 bits (335), Expect = 8.1e-30
Identity = 173/732 (23.63%), Postives = 299/732 (40.85%), Query Frame = 0
Query: 21 WQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMKDLSQIKGVGK----WILKQV 80
W + + AT KG K + KA +V N I K L ++ VG + K++
Sbjct: 15 WLQEAAINATKKG--TKAAILYNKALGSVRNYPLPINDPKTLKSVQFVGDKTCIHLSKKL 74
Query: 81 QDFFKSGS---------------------EGSEPEDLA-GKGKRAKGTKRYVPQKNSVAY 140
+++ K + E S+ +L K K+ + K+Y+P+K S Y
Sbjct: 75 EEYCKLNNFELPVAFGGLINGGVGEKRKHEVSDTSNLPDAKPKKQRKQKQYIPRKRSGGY 134
Query: 141 ALLVTLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVP 200
A+L+ LY + K+E+I A P
Sbjct: 135 AILLALY--------------------------FGDKKKTGLTKEEIIQRA-------TP 194
Query: 201 IGPEKGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAK-YMLTEEGQQVARECL 260
+ K P S ++YS WS +K+L + LV S +K Y LTEEG ++A++
Sbjct: 195 YSDKSFKSNP-----SANEFYSAWSSIKSLQTHDLVDSSGRSSKSYFLTEEGYELAKQ-- 254
Query: 261 MRSGLLDSTTNFDNAKGSSVLESCDLSDRNAVNLDSD-------VEVTSPPVGQSRQNKS 320
L F+++ ++ + DLS N V + D ++ S P+ +++ NK
Sbjct: 255 -----LKDAEGFESSPITNHI--ADLSFDNQVRVTPDSSYSKISQQLDSSPLMKTKNNK- 314
Query: 321 TDVPLESLERF----MRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQ 380
+R+ R+ ++ ++L+ +P S L P LRE L
Sbjct: 315 --------DRYGLGPSRLSSTRERILDL-----SSSPRLISSPLKPKDFV-LRESL---- 374
Query: 381 VEAVGRESRSMGSSHEGHMP--KTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNI 440
SSH P I E N + + H ++ D ++ +
Sbjct: 375 ------------SSHNSTRPLQDRESISKAEDGINTKTKARLVHDASKRIYDGTNYDIWV 434
Query: 441 LSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDG 500
GE + ++LI+D+RE S R R + + K++ ++R L VGD
Sbjct: 435 P-------GE-----FEIILIIDNRE---IRSQRDRDFFQTRLTSLKVECDVRPLSVGDV 494
Query: 501 IWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVE---- 560
+W A+HK E +L++I ERK +DDL SI+D R+++QK +L + G+K+ YLVE
Sbjct: 495 VWTAKHKKTGREVILNYICERKRLDDLVSSIKDGRFQEQKNRLKKSGMKQFYYLVEDVVT 554
Query: 561 GDPNS-SEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETD-- 620
D N + +++I+TA T + F ++R + DT+ LT+ I + +T+
Sbjct: 555 SDMNKFGDMSDAIQTAMSMTMTISNFYLKRFKSIEDTIAFLASLTQVIKDQFAKNKTNLL 614
Query: 621 --------ERLSHSDVCPRFNEFIK------KCQEL-----------DKMTVSDVFALQL 674
+ +S + +F E + +C L MTV + F L L
Sbjct: 615 VLKARSIKNQAEYSSLIAKFKEKFENRSTSYECAHLFSTFQDSMGKTGMMTVKETFILML 650
BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match:
Q7SXA9 (Crossover junction endonuclease MUS81 OS=Danio rerio OX=7955 GN=mus81 PE=1 SV=1)
HSP 1 Score: 131.0 bits (328), Expect = 5.2e-29
Identity = 158/592 (26.69%), Postives = 237/592 (40.03%), Query Frame = 0
Query: 95 KGKRAKGTKRYVPQKNSVAYALLVTLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNE 154
K K AK + YVPQK S YA+L+TLYR+
Sbjct: 127 KEKGAKKKREYVPQKRSGGYAVLLTLYRH-----------------------MQMPGSKG 186
Query: 155 YMRKQELIDAAEASGLSRVPIGPEKGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSS 214
+M + EL EA L EK P GS Y+ WS + TLI + LVVK+
Sbjct: 187 FMFRNEL--QTEAQPLC------EKSFTVP-DLGSK----YTAWSSVSTLIQKELVVKTH 246
Query: 215 CPAKYMLTEEGQQVARECLMRSGLLDSTTNFDNAKGSSVLESCDLSD-RNAVNLDSDVEV 274
PA+Y LT++G +A + D+ + + E D D +N V+L
Sbjct: 247 NPARYSLTDQGLSLAEK-------------LDSEETGTRHEDVDSQDGQNVVDL------ 306
Query: 275 TSPPVGQSRQNKSTDVPLESLERFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCR 334
+E ED + SS PAV
Sbjct: 307 ------------------------------------TLEEEDEDEEKESWSSERPAVA-- 366
Query: 335 LREDLVYGQVEAVGRESRSMGSSHEGHMPKTVVIDDGERSFNLRACSSSNHAVTESCRDD 394
L Q + ES MG S +T + ++L S
Sbjct: 367 ----LPVNQARGLMSESDPMGKSQTSETGRTAM------GWHLSPGS------------- 426
Query: 395 VKANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIR 454
Y++VL +D E S R + +++ + + + ++R
Sbjct: 427 ----------------------YDIVLCVDLCETTGGSSVRKQELVKEL-QRNSVTFDVR 486
Query: 455 RLPVGDGIWIARHK----------HLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKL 514
+L VGD +W+AR + + E VLD+I+ERK +DDL SI D R+R+QK +L
Sbjct: 487 KLNVGDFLWVARERVTPVPGQLRPPVGKELVLDYIIERKRMDDLCGSIIDGRFREQKFRL 546
Query: 515 LRCGLKKMIYLVE----GDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYL 574
RCGL+K IYLVE + S +++ A T++++GF V+R ++ +
Sbjct: 547 KRCGLRKPIYLVEECGSAAAHLSIPESTLQQAIVNTQVVDGFFVKRVQDAKESAAYLTIM 573
Query: 575 TRAITQYYIS--------------QETDERLSHSDVCP--RFNEF----IK-KCQELDKM 634
TR + + Y + + E++ + C F EF IK KCQ
Sbjct: 607 TRYLQKLYQNCTLLCRSRELEGDGEAESEKMVANLSCSLMAFTEFNYGAIKNKCQ----- 573
Query: 635 TVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEML 651
TV +VFA QLMQ+ V+ + A AVL Y T+ SL +AY TE+E++L
Sbjct: 667 TVREVFARQLMQISGVSGDKAAAVLKHYSTVSSLLQAYDKCSSE-TEKEKLL 573
BLAST of Spg009758 vs. ExPASy Swiss-Prot
Match:
P87231 (Crossover junction endonuclease mus81 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mus81 PE=1 SV=2)
HSP 1 Score: 127.9 bits (320), Expect = 4.4e-28
Identity = 138/580 (23.79%), Postives = 233/580 (40.17%), Query Frame = 0
Query: 81 KSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTLYRYNGQIAISNVEFLFELIC 140
KS SE S + + K + K YVP S AY++L LY
Sbjct: 94 KSSSETSSEKPRSVKKPTTRKRKVYVPSYRSGAYSILCALYML----------------- 153
Query: 141 NVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKGKGKPSQFGSSP--RDWYSGW 200
N +E+ K +++ A+ S FGS+ Y+ W
Sbjct: 154 ----------NKHEFATKPQIVTMAQPY--------------CDSSFGSATDRNMRYTAW 213
Query: 201 SCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLDSTTNFDNAKGSSVLESCD 260
S MKTLI++ LV ++ P+KY LT++G++V C+ + + DS SV +S
Sbjct: 214 SAMKTLITKNLVYQTGHPSKYCLTDDGEEV---CIRLAKVDDSFQRKHTVSNFSVSKSD- 273
Query: 261 LSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRMGYSKHQVLNAFKEVSEDN 320
D D + PP + NK+ E + H EVS+
Sbjct: 274 ---------DHDSSLCQPPNFVTSINKAGSSSDHGGELHVTYCPVDH------NEVSDG- 333
Query: 321 PYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGHMPKTVVIDDGERSFNLRA 380
+ D+ QV+ S+ H+ H I + E+ +L
Sbjct: 334 ---------------VETDIDVDQVD-------SLTGIHDHH------IINNEQLIDL-- 393
Query: 381 CSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRM 440
+ + ++N++ L + + F V L++D RE S R +
Sbjct: 394 -------TEQEKKQPNESNLSNLKIETVLFSN-----CTVFLLIDTRE---IRSPLDRNL 453
Query: 441 -IENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYR 500
I+ + + F + ++R L +GD +W+AR E VLDF+VERK DDL SI+D R+
Sbjct: 454 IIDKLTNDFGVNCQVRSLELGDALWVARDMESGQEVVLDFVVERKRYDDLVASIKDGRFH 513
Query: 501 DQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYG 560
+QK +L + G++ + Y++E ESI+TA T++ + F V+ T L ++
Sbjct: 514 EQKARLKKSGIRSVTYILEESSYDESFTESIRTAVSNTQVDQLFHVRHTRSLEHSVSLLA 567
Query: 561 YLTRAITQYYISQET------------------DERLSHSDVCP---RFNEFIKKCQELD 620
+T+ I +Y ++T ++ L P ++ F +
Sbjct: 574 EMTKQINLFYEKRKTLAVIPDLSIEAKTYESLREQLLKIDPSTPYHISYHAFSSVLSKSS 567
Query: 621 KMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAY 637
+TV D+F LM + ++ A+ + YPT + L AY
Sbjct: 634 TLTVGDIFIRMLMTIKGISASKAIEIQKKYPTFMHLFEAY 567
BLAST of Spg009758 vs. ExPASy TrEMBL
Match:
A0A6J1GYZ8 (crossover junction endonuclease MUS81 OS=Cucurbita moschata OX=3662 GN=LOC111458164 PE=3 SV=1)
HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 576/675 (85.33%), Postives = 611/675 (90.52%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1 MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQVQ FK+GSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61 DLSQIKGVGKWILKQVQVIFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKP QFGSS RDWYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
S NFD KGSS+LESC +SDR+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCGISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+RS GSS+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTGSSNEGH 360
Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
+PK VV D+GERSFNLRACSSSNH++TES +D VK N NILSMPPLSFGEKFED Y+VVL
Sbjct: 361 VPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSVVL 420
Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
ILDDREQFANHSSRSRRMIEN+CSQFKI+IEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIEIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DERLSH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMTV 600
Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
SDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLLD NVT+QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNVTQQEEMLRRQSNNLINA 650
Query: 661 GASRNIFHLVWGCGK 676
ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 650
BLAST of Spg009758 vs. ExPASy TrEMBL
Match:
A0A6J1JV85 (crossover junction endonuclease MUS81 OS=Cucurbita maxima OX=3661 GN=LOC111488639 PE=3 SV=1)
HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 574/675 (85.04%), Postives = 608/675 (90.07%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MDRQR+PVVCPENE+LAAFMWQKRLEMA TPKG SDKVDMTLYKAYFNVCNSK SIKSMK
Sbjct: 1 MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGTSDKVDMTLYKAYFNVCNSKDSIKSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQVQ FFKSGSEGS PE++AGKGK+ KGTKRYVPQKNSVAYALL+TL
Sbjct: 61 DLSQIKGVGKWILKQVQVFFKSGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKP QFGSS R+WYSGW+CMKTL+SRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTREWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
S NFD KGSS+LESCD+S R+A +LDSDVEVTSP + +S QNKS DVPLESLERFMRM
Sbjct: 241 SEKNFDTMKGSSILESCDISGRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
GYSKHQVL++FKEVSEDNP KDISSLWPAVLCRLREDLVYGQVEAVGRE+R GSS+EGH
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRLTGSSNEGH 360
Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
+PK V+ D+GERSFNLRACSSSN ++TESC+D VK NMNILSMPPLSFGEKFED Y+VVL
Sbjct: 361 VPKIVISDNGERSFNLRACSSSNQSITESCKDVVKENMNILSMPPLSFGEKFEDAYSVVL 420
Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
ILDDREQFANHSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE
Sbjct: 421 ILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
RKNIDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
FDVQRTSGL+DTLKKYGYLT AITQYYI Q +DER SH DVCPR + FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERPSHFDVCPRLDAFIKKCQELDKMTV 600
Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
SDVFALQLMQVPQVTEE+AV VLDLYPTLLSLARAYSLLD NVT QEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEIAVTVLDLYPTLLSLARAYSLLDDNVTHQEEMLRRQSNNLINA 650
Query: 661 GASRNIFHLVWGCGK 676
ASRNIFHLVWG GK
Sbjct: 661 AASRNIFHLVWGSGK 650
BLAST of Spg009758 vs. ExPASy TrEMBL
Match:
A0A1S3BFN6 (crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489485 PE=3 SV=1)
HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 575/675 (85.19%), Postives = 610/675 (90.37%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MDRQR PV CPENE+LAAFMWQKRLEMAATPKGISDKVDMTLYKAYFN+CNSK SI+SMK
Sbjct: 1 MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQVQDFFKSGSEGS PE+LAGKGK+ KGT+RYVPQKNSVAYALL+TL
Sbjct: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDAAEASGLSRV I PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKS+CPAKYMLTEEGQQVARECLMRSGLL
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
S+ NFDN KGSS+LESCD+S+ +A LDSDVEVTSP V Q +NK+ DVPLESLERFMRM
Sbjct: 241 SSKNFDNIKGSSILESCDISNHSANYLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEGH 360
GYSKHQV+N+FKEVS+DNP KD SSLWPAVLCRLREDLVYGQVEAVGRE+RSMG SHEG
Sbjct: 301 GYSKHQVINSFKEVSKDNPNKDTSSLWPAVLCRLREDLVYGQVEAVGRENRSMG-SHEGS 360
Query: 361 MPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVVL 420
PK VV D+GERSFN RACSSSN ++ ES RD VK + NILSMPPLSFGEKFED YNVVL
Sbjct: 361 EPKNVVNDNGERSFNSRACSSSNPSIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVVL 420
Query: 421 ILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVE 480
ILDDREQFA HSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIVE
Sbjct: 421 ILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIVE 480
Query: 481 RKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
RK+IDDLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILEG
Sbjct: 481 RKDIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILEG 540
Query: 541 FDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMTV 600
FDVQRTSGL+DTLKKYGYLT AITQYYI Q +D+R+SHSDVCPR ++FIKKCQELDKMTV
Sbjct: 541 FDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMTV 600
Query: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 660
SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA
Sbjct: 601 SDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINA 649
Query: 661 GASRNIFHLVWGCGK 676
GASRNIFHLVWG G+
Sbjct: 661 GASRNIFHLVWGSGR 649
BLAST of Spg009758 vs. ExPASy TrEMBL
Match:
A0A0A0KWV2 (ERCC4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623550 PE=3 SV=1)
HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 571/676 (84.47%), Postives = 608/676 (89.94%), Query Frame = 0
Query: 1 MDRQRLPVV-CPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSM 60
MDRQR P V CPENE+LA FMWQKRLEMAATPKGISDKVDMTLYKAY NVCNSK SI SM
Sbjct: 1 MDRQRSPAVFCPENEDLAEFMWQKRLEMAATPKGISDKVDMTLYKAYLNVCNSKVSITSM 60
Query: 61 KDLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVT 120
KDLSQIKGVGKWILKQVQDFFKSGS+GSEPE+LAGKGK+ KGT+RYVPQKNSVAYALL+T
Sbjct: 61 KDLSQIKGVGKWILKQVQDFFKSGSDGSEPEELAGKGKKTKGTRRYVPQKNSVAYALLIT 120
Query: 121 LYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEK 180
LY R T+NGNEYMRKQELIDAAEASGLSRV I PEK
Sbjct: 121 LY-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEK 180
Query: 181 GKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLL 240
GKGKPSQFGSS RDWYSGWSCMKTLISRGLVVKS+CPAKYMLTEEGQQVARECLMRSGLL
Sbjct: 181 GKGKPSQFGSSSRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLL 240
Query: 241 DSTTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMR 300
S+ NFDN KGSS+LESCD+S+ +A LDSDVEVTSP V QS ++KS DVPLESLERFMR
Sbjct: 241 SSSKNFDNTKGSSILESCDISNHSANYLDSDVEVTSPSVSQSTKHKSIDVPLESLERFMR 300
Query: 301 MGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGSSHEG 360
MGYSKHQV+N+FKEVS+DNP KDISSLWPAVLCRLREDLVYGQVEA+GRE+RSMGSS EG
Sbjct: 301 MGYSKHQVINSFKEVSKDNPNKDISSLWPAVLCRLREDLVYGQVEAIGRENRSMGSSLEG 360
Query: 361 HMPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVV 420
PK VV +GERSF+ RACSSSN ++ ES RD VK N NILSMPPLSFGEKFED YNVV
Sbjct: 361 SEPKNVVNVNGERSFSSRACSSSNPSIIESRRDSVKGNTNILSMPPLSFGEKFEDCYNVV 420
Query: 421 LILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
LILDDREQFA HSSRSRRMIEN+CSQFKIQIEIRRLPVGDGIW+ARHKHLDNEYVLDFIV
Sbjct: 421 LILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWVARHKHLDNEYVLDFIV 480
Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILE 540
ERK+++DLRCSIRDNRYRDQKLKLLRCGLK+MIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKDVEDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
Query: 541 GFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMT 600
GFDVQRTSGL+DTLKKYGYLT AITQYYI Q +D+R+SHSDVCPR ++FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMT 600
Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLIN 660
VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSL RAYSLLDGNVTEQEEMLRR+SNNLIN
Sbjct: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLVRAYSLLDGNVTEQEEMLRRRSNNLIN 651
Query: 661 AGASRNIFHLVWGCGK 676
AGASRNIFHLVWGCGK
Sbjct: 661 AGASRNIFHLVWGCGK 651
BLAST of Spg009758 vs. ExPASy TrEMBL
Match:
A0A6J1CEX9 (crossover junction endonuclease MUS81 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010103 PE=3 SV=1)
HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 573/676 (84.76%), Postives = 606/676 (89.64%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
M+RQRLPVVCPENE+LA FMWQKRLEMAATPKGIS++V+MTLYKAYFNVCNSKASIKSMK
Sbjct: 1 MERQRLPVVCPENEDLAGFMWQKRLEMAATPKGISERVEMTLYKAYFNVCNSKASIKSMK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGKGKRAKGTKRYVPQKNSVAYALLVTL 120
DLSQIKGVGKWILKQVQDFFKSGSE SEP DLAGKGK+ KGTKRYVPQKNSVAY LL+TL
Sbjct: 61 DLSQIKGVGKWILKQVQDFFKSGSEDSEPADLAGKGKKKKGTKRYVPQKNSVAYTLLITL 120
Query: 121 YRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPEKG 180
Y R T+NGNEYMRKQELIDAAEASGLSRVPI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVPIAPEKG 180
Query: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLD 240
KGKP+QFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYM TEEG+QVARECL RSGL +
Sbjct: 181 KGKPAQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMPTEEGKQVARECLERSGLSN 240
Query: 241 STTNFDNAKGSSVLESCDLSDRNAVNLDSDVEVTSPPVGQSRQNKSTDVPLESLERFMRM 300
ST NFDN KGSS+L+SCD+SD + LDS+VEVTSP G SRQNKS +VP ESL+RFMRM
Sbjct: 241 STMNFDNDKGSSILKSCDISDHSVDCLDSEVEVTSPCAGVSRQNKSINVPPESLQRFMRM 300
Query: 301 GYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMG-SSHEG 360
GYSK+QVL+AFKEVSEDNP KDISSLWPAVLCRLRED VYGQVEAVGRE+R +G SS+EG
Sbjct: 301 GYSKNQVLDAFKEVSEDNPNKDISSLWPAVLCRLREDHVYGQVEAVGRENRFLGSSSYEG 360
Query: 361 HMPKTVVIDDGERSFNLRACSSSNHAVTESCRDDVKANMNILSMPPLSFGEKFEDLYNVV 420
HM KT VID+ ERSF LR+CSSS+HA+TES RD VKANMNILSMPPLSFGEKFED Y VV
Sbjct: 361 HMRKTAVIDNVERSFTLRSCSSSDHAITESSRDCVKANMNILSMPPLSFGEKFEDAYKVV 420
Query: 421 LILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
LILDDREQF N SSRSRRMIEN+CSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV
Sbjct: 421 LILDDREQFVNQSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILE 540
ERKNIDDLRCSIRDNRY+DQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKNIDDLRCSIRDNRYKDQKLKLLRCGLKKMIYLVEGDPNSSEAAESIKTACFTTEILE 540
Query: 541 GFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHSDVCPRFNEFIKKCQELDKMT 600
GFDVQRTSGL+DTLKKYGYLT AIT YYISQ + ERLS SDVCP NEFIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITHYYISQASSERLSRSDVCPPLNEFIKKCQELDKMT 600
Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLIN 660
VSDVFALQLMQVPQVTEEVAVAV+DLYPTLL LARAYSLLDGNV+EQEEMLRRQS NLIN
Sbjct: 601 VSDVFALQLMQVPQVTEEVAVAVVDLYPTLLLLARAYSLLDGNVSEQEEMLRRQSKNLIN 651
Query: 661 AGASRNIFHLVWGCGK 676
AGASRNIFHLVWG GK
Sbjct: 661 AGASRNIFHLVWGSGK 651
BLAST of Spg009758 vs. TAIR 10
Match:
AT4G30870.1 (Restriction endonuclease, type II-like superfamily protein )
HSP 1 Score: 674.9 bits (1740), Expect = 6.9e-194
Identity = 366/694 (52.74%), Postives = 472/694 (68.01%), Query Frame = 0
Query: 1 MDRQRLPVVCPENEELAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKASIKSMK 60
MD +R V+CPEN LAA++ QK+ E A PKG+S+ ++ T K Y +VC++K I ++K
Sbjct: 1 MDDER-RVLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLK 60
Query: 61 DLSQIKGVGKWILKQVQDFFKSGSEGSEPEDLAGK--GKRAKGTKRYVPQKNSVAYALLV 120
DLSQIKG GKW++K ++ +F + E SE EDL GK+A G KRY+PQ+NSV YALL+
Sbjct: 61 DLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLI 120
Query: 121 TLYRYNGQIAISNVEFLFELICNVRFIIRSTSNGNEYMRKQELIDAAEASGLSRVPIGPE 180
TL+ R T+NG E+MRKQELIDAA+A+GLS P+GPE
Sbjct: 121 TLH-------------------------RRTTNGKEFMRKQELIDAADANGLSHAPVGPE 180
Query: 181 KGKGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSSCPAKYMLTEEGQQVARECLMRSGL 240
KGKGK + G S R+WYSGWSCM TLI +GLVVKSS PAKYMLT EG++VA EC++RSGL
Sbjct: 181 KGKGK-AGLGHSKREWYSGWSCMTTLIQKGLVVKSSNPAKYMLTVEGREVANECILRSGL 240
Query: 241 LDSTTNFDNAKGSSVLESCDLSDRN---AVNLDSDVEVTSPPVGQSRQNKSTDVPLESLE 300
DS + ++ ++N + + ++ P R + +P + LE
Sbjct: 241 PDSVDILSVDEMDPTPQAKKTPNQNPTCSFTMREELPYVDP-----RCRAQSAIPSDILE 300
Query: 301 RFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWPAVLCRLREDLVYGQVEAVGRESRSMGS 360
+F GYSK QV+ AF+EVS+ + KD S+LW +V+C LR+ VY +SR+
Sbjct: 301 KFTPFGYSKEQVVAAFREVSDGSGDKDPSTLWLSVMCHLRQAEVYNSCP----DSRNSKK 360
Query: 361 SHEGHMPKTV--VIDDGERSFNLRAC----------SSSNHAV----TESCRDDVKANMN 420
G + V +G R+ R+C S S+HAV + D K N
Sbjct: 361 DSSGPFKSQIRQVDLEGSRAKKFRSCNDGSTLNPCSSGSSHAVKACSSSLASDGTKGITN 420
Query: 421 ILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKIQIEIRRLPVGD 480
I +PPL FGE FE+ Y+V+LILDDRE+FA SRSR ++EN+CS+F I+IE+RRLPVGD
Sbjct: 421 IPRLPPLQFGETFEEAYDVILILDDREKFATKGSRSRNIVENICSEFNIKIEVRRLPVGD 480
Query: 481 GIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKKMIYLVEGDP 540
IWIARHK+L+ EYVLDFI ERKN+DD+R SIRDNRYRDQKL+L R G KK+IY++EGDP
Sbjct: 481 CIWIARHKYLETEYVLDFIAERKNVDDMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDP 540
Query: 541 NSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYISQETDERLSHS 600
N S+AAESIKTACFTTEILEGFDV RT GL +TL+KYGYLT++I QYY + D S
Sbjct: 541 NHSDAAESIKTACFTTEILEGFDVLRTHGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKG 600
Query: 601 DV-CPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSL 660
CP F+ F+K+CQ+LDKMT+SDVFA+QLMQVPQVTEE+A+AVLD+YPTLLSLA AYS
Sbjct: 601 AASCPSFDSFVKRCQDLDKMTISDVFAIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSH 658
Query: 661 LDGNVTEQEEMLRRQSNNLINAGASRNIFHLVWG 673
L+ +V+ QEEMLR +SNN+I A AS+NIF LVWG
Sbjct: 661 LEADVSAQEEMLRNRSNNVICASASKNIFKLVWG 658
BLAST of Spg009758 vs. TAIR 10
Match:
AT5G39770.1 (Restriction endonuclease, type II-like superfamily protein )
HSP 1 Score: 439.5 bits (1129), Expect = 4.9e-123
Identity = 280/651 (43.01%), Postives = 367/651 (56.37%), Query Frame = 0
Query: 31 PKGISDKVDMTLYKAYFNVCNSKASIKSMKDLSQIKGVGKWILKQVQDFFKSGSEGSEPE 90
PKG+S+ +M KAY NVC++K SI ++KDLS+IKG GK+++K +Q +F +G+ SE E
Sbjct: 762 PKGLSEYQEMKFVKAYRNVCDAKDSIDTLKDLSKIKGFGKYMVKLMQGYFVTGAASSEQE 821
Query: 91 DLAGK--GKRAKGTKRYVPQKNSVAYALLVTLYRYNGQIAISNVEFLFELICNVRFIIRS 150
L G GK+AKG++ Y PQ+N+ AYALL TL+R+
Sbjct: 822 ALPGNRAGKKAKGSEPYKPQRNTAAYALL-TLHRF------------------------K 881
Query: 151 TSNGNEYMRKQELIDAAEASGLSRVPIGPEKGKGKPSQFGSSPRDWYSGWSCMKTLISRG 210
T+NG + MRKQ+L+DAA+ASGLS V I PEKGKGK + G+S R+WYSGWSCM LI +G
Sbjct: 882 TANGKKLMRKQKLMDAADASGLSHVSIAPEKGKGK-AGLGTSKREWYSGWSCMSKLIEKG 941
Query: 211 LVVKSSCPAKYMLTEEGQQVARECLMRSGLLDSTTNFDNAKGSSVLESCDLSDRNAVNLD 270
LVVK S PAKYMLT EG+ VA + RS +G S
Sbjct: 942 LVVKFSNPAKYMLTVEGRNVADDLDPRS------------RGQS---------------- 1001
Query: 271 SDVEVTSPPVGQSRQNKSTDVPLESLERFMRMGYSKHQVLNAFKEVSEDNPYKDISSLWP 330
+ + P ++++ +TD
Sbjct: 1002 ---SIPAGPSSRAQEFLATDT--------------------------------------- 1061
Query: 331 AVLCRLREDLVYGQVEAVGRESRSMGSSHEGHMPKTVVIDDGERSFNLRACSSSNHAVTE 390
GQV+ G ++ SS P + I S L+ACSSS
Sbjct: 1062 ------------GQVDLEGSRAKKFRSS----SPCSSSI-----SHALKACSSS------ 1121
Query: 391 SCRDDVKANMNILSMPPLSFGEKFEDLYNVVLILDDREQFANHSSRSRRMIENVCSQFKI 450
D + I +PPL FGE FE++Y+V+LILDDREQFA
Sbjct: 1122 LASDGTEGVTTIPRLPPLRFGEAFEEVYDVILILDDREQFATKG---------------- 1181
Query: 451 QIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGL 510
HK+ +EYVLDFIVERKN+DD+R SI DNRY+DQKL+L R G+
Sbjct: 1182 -----------------HKYQQDEYVLDFIVERKNVDDMRSSIMDNRYKDQKLRLQRSGI 1241
Query: 511 KKMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLADTLKKYGYLTRAITQYYI 570
KK+IY++EGDPN S+ AESIKTACFTTEILEGFDV RT+GL +TL+KYGYLT++I QYY
Sbjct: 1242 KKLIYILEGDPNQSKTAESIKTACFTTEILEGFDVVRTNGLGETLRKYGYLTKSIYQYYK 1255
Query: 571 SQETDERLSHSDVCPRFNEFIKKCQELDKMTVSDVFALQLMQVPQVTEE---------VA 630
S D++ +D CP +++F+++C +L KMT+SDVFA+QLMQV + + A
Sbjct: 1302 SLVNDDQRKVADSCPSYDDFVQRCPDLGKMTISDVFAIQLMQVLSLAKPSFGFLSATFFA 1255
Query: 631 VAVLDLYPTLLSLARAYSLLDGNVTEQEEMLRRQSNNLINAGASRNIFHLV 671
+AVLD Y TL SLA AYS LD NV+ QEEMLR QSNN+I A AS+NI+ LV
Sbjct: 1362 IAVLDKYKTLRSLASAYSKLD-NVSAQEEMLRNQSNNVICASASKNIYKLV 1255
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038892703.1 | 0.0e+00 | 86.58 | crossover junction endonuclease MUS81 isoform X1 [Benincasa hispida] | [more] |
XP_022956424.1 | 0.0e+00 | 85.33 | crossover junction endonuclease MUS81 [Cucurbita moschata] | [more] |
XP_023514312.1 | 0.0e+00 | 85.48 | crossover junction endonuclease MUS81 [Cucurbita pepo subsp. pepo] | [more] |
XP_022992270.1 | 0.0e+00 | 85.04 | crossover junction endonuclease MUS81 [Cucurbita maxima] | [more] |
XP_008446914.1 | 0.0e+00 | 85.19 | PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo] >XP_0... | [more] |
Match Name | E-value | Identity | Description | |
Q5W9E7 | 9.7e-193 | 52.74 | Crossover junction endonuclease MUS81 OS=Arabidopsis thaliana OX=3702 GN=MUS81 P... | [more] |
Q8GT06 | 2.9e-152 | 46.86 | Crossover junction endonuclease MUS81 OS=Oryza sativa subsp. japonica OX=39947 G... | [more] |
Q6BJ48 | 8.1e-30 | 23.63 | Crossover junction endonuclease MUS81 OS=Debaryomyces hansenii (strain ATCC 3623... | [more] |
Q7SXA9 | 5.2e-29 | 26.69 | Crossover junction endonuclease MUS81 OS=Danio rerio OX=7955 GN=mus81 PE=1 SV=1 | [more] |
P87231 | 4.4e-28 | 23.79 | Crossover junction endonuclease mus81 OS=Schizosaccharomyces pombe (strain 972 /... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GYZ8 | 0.0e+00 | 85.33 | crossover junction endonuclease MUS81 OS=Cucurbita moschata OX=3662 GN=LOC111458... | [more] |
A0A6J1JV85 | 0.0e+00 | 85.04 | crossover junction endonuclease MUS81 OS=Cucurbita maxima OX=3661 GN=LOC11148863... | [more] |
A0A1S3BFN6 | 0.0e+00 | 85.19 | crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... | [more] |
A0A0A0KWV2 | 0.0e+00 | 84.47 | ERCC4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623550 PE=3 ... | [more] |
A0A6J1CEX9 | 0.0e+00 | 84.76 | crossover junction endonuclease MUS81 isoform X1 OS=Momordica charantia OX=3673 ... | [more] |
Match Name | E-value | Identity | Description | |
AT4G30870.1 | 6.9e-194 | 52.74 | Restriction endonuclease, type II-like superfamily protein | [more] |
AT5G39770.1 | 4.9e-123 | 43.01 | Restriction endonuclease, type II-like superfamily protein | [more] |