Spg009742 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009742
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncucumisin-like
Locationscaffold7: 9516796 .. 9524208 (+)
RNA-Seq ExpressionSpg009742
SyntenySpg009742
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTTTTTTTTTTGAGTTACTAATGAGTGTGTTTGAAAGGCAGGTGTATGTTGTATACATGGGCAAATCATCTGAAGGAGGTTTCACAGCAGCATCTGAACGACATACCAATATGCTTCAACAAGTTCTTACCACCAGGTTACGATGCTTTTAGTGGGAATCATTTTGACTTTTCAAGTTAGCTCATAATGTTTATTTTATTTTATTTTTTTTGGGTAAAAATTATCTTTAAATGCTTTTAGTGGGAATCATTTTGACTTTTCAAGTTAGCAATTTCTTGAATAAGGACGATGTAGATATGACATACATCATATGGATTATTACATTTGAGAAGATATATTTTGTGGATGAAAAGGTTCATATAGCTCATAATTACAAAAGGCTCACCATAATACTATATCATTTTTTTTTTTAATTTTAAATTAAATCCTTACACAATAATATTGTTAAAATTTAACAATAATATAATGAAAAGGCAATGTTATATTTGAAATATATGTTTTGTGATATATTTACTTGGTAAGTCTTTTTATTTCTTTTTCCCTTTTTTCTTCTTTTAATGGGAATGAGATATATGAAGTGTACTAATTAGATAATACCTTACTAGGTAGAGTTTGGGGTGTTAATTCAGATAGGGTTAGTTGAGTTTTTTTTAATAGAGCAGTGAAGATAAATATATTTAACATTAAAACAGTGTCTACTTGTGTAAAATAATATCTTTAAATGTCATATCACTAATATATTATACAATTTTAATTGTGTTAAAATAAAGGAATGTTTATCATCTTACAATATTATAATTCAAATAGGGAGTAGAAATTAAAACTTATAACTTTTAACAAAGTTATCAATGTGTTATCTTAATTGAGTTACGTTCAAGTTTATAATGTTAGGGTATGACTAAATGTTCAAAAAAAAAATCAGAAATTCTTCTATATTCATAAATATGATTAACTAATTAACCTTCTTTTTCTTTTATATATACAAAAACTAATTTCCCAATAGAAACAACGTAAAAATCTCATAGGAATTAACTCTGAGTAATGAACTTTGATCTCAGTGATGCATCCAAATCTCTGGTGTATAGCTACCACCGGAGCTTCAGCGGCTTTGCAGCGAGGCTCAACGAGGAGGAGGCTCGGAAACTTGCCGGTGGGTTCTTGTAAAAAATGCATGTTTGGAAGTGATTTTAAAATGGTTAAAATCATTTTTGTCATATTCAAAATCACTCCCAAACATGTACTTAATAATTCAAAGTCAATTTTATTGATATGAAAATCACGTTTAAAAGTGTAAAATCAAACATCAAATTGATTTTAAATGATTAAGGGTGCATGTTTCGGAATTATTTTGAACATGACAAAAGTGATTTTAGTCATTTCAAAATCACTCCCAAACATGCACTAAATATGATATTTCTTAAGTTTAGTTCAAGTATTTAAAAATTTTCAATTTAGTGTTTCAAAAAAAAAAAAAATCAATTTTTTCCCTTTAGTTGCTCACCTTTGGTTGAATATTAAAATCGGTTATTGATTATAAACTTACCTAGTATAATATAATATTTGGTGAAAATTCTAAAAAATGTCATTTAAGATAATTAAACTCATAATTTTCATCAATTTCTTTAAAAATTGAAGGAAAGAATTAGAAACTTTCCATTCTTTAATTAGTTTGAATGATTTTTTTTTAGTACATCAACATTCGGGAGTGAAGGGATTCGAACCTCTGACTTCATGGTTGAGGGTACATGCCAATTACCGCTAGCTAAGCTCGCTTTGGCGGTTTGAATGACTTTTTAAGTGTACCTAAAAACATTTCTTTTGCACTTAAAACATTTTTTCAAACACTTCATTTCAAACCGAACTTAAATATAAATTTATCGTATAGTGAATTTTAATATTGAATTGTGAGGGACTAAATCGAAAAACCCTCAAATAATAGGAAGTAAAAATATGTATTTTGGTGTTAAAAAGAAAGTTTTCTCATTGGGAAAAAAGAAATAGTCTTATTTGTTTAAAGTTTTAGAATTTTGATGGGTTTTGGATTTTTTCAGAAATGGAGGAAGTTGTATCTGTGTTTCCGAGCGAAAAAAAACAGCTTCACACGACAAGATCATGGGATTTCATGGGTTTATTCGAAGAAGCTCGAAGAACGAGCTTGGAGAGCGATCTGGTCGTCGGAATGTTGGACACTGGAATTTGGCCGGAATCTGAAAGCTTTTCCGATGAAGGGTTTGGTCCGCCGCCGGCGAAGTGGAAAGGCGAATGCCAACCGTCTTCCAATTTCACCTGCAACAAGTAATCCTATTTTAAACCCTTCAACCTAAGGATTTCTTTAGTCTTAGGTTAAATTAGTATTCTTTTTGTTGGTATAGATTTTCTTTTTACTTTTGTCACATGATTTTGAAACTGTTATTATAATTTTTATGAACCATTTTTTTTTTTTGTTGAGTACATCAACCTTTGGAGGTGGGGGATTCGAACCTCTCACCTCTTAGTCGATGGTACATGTCAATTATCACTGAGCTAGACTCGTTTTGGCAATTTGGATGAACTACTTATGGTGTGTTTGGTTGGTGATCTGAAAACAAATGATTTAATAAAAACAAGATTATATTTTATATTTTCCAGATATGTGTTTGATAACAAATTTGTAAATTGGATTTCAATTTAAATGATTGTTCATATTTGATGATATATTTATAAACCATAAACTAGTTGTAGTTATTAAAAAAAAATATATATTAAGTTAAATACAAACAATTATTAACTAATTTATAAACCTTTTTGATGTTATGAAATTTTTATAGTTATTATTTTATGGTATCATATAATTTATAATATATTATATTATACGTGGGTTAATTTAACAAAAATGACTTGGATTCATAGTAAAATTAAATATTTATTAAAGTTTTGGGTAGTTGCATGGATGACAAATTGAACCAAATTAATAGCAAATATAGCACAGTTGCAAACTAATTGCAAATATAGCAAAATTAATTGTACAATACCAAAAAACGGTTGTCAGATTCTATTCGTGTCTATCACCGATAGACCGATTATTCAAAGTATATCAGTGTTTATCTAGACTTTGATTAATCAAAATCTATTAGTGACTTGATATTGATAGAATCAAAGTCTATCAGTGATAGTCATTGATAGATTTTGATTAATCAAAATTTATCAGCGATAGACACTGATAGACTCTGAATAATCGGAGTCTATCAGTAGACTGATAGATTTTGATTAATCAGAGTCTATCGCTGACAAACTCTAATAATCGAAGTCTATCAGATAGATTCTAATTAGCCAGAGTCTATCAGTGTAGTATTATACTCTGATAGATTTTGCTATATTTGCTATTAATTTGGTCCAATTTACCACCCACTACAAATTCTCTAAAGTTTATATCATTTTTAATTTAATTTTGTATTTTAAATTTTCTAATTGAAAATCCGAATACATTGGAAATATATATAAAAAAAGAAAATTGTTTTCAAGAATGTTCACTATTTGATCATGAAATTCGAAAACAGTTTTAAAAAATAATCGGTGAAACACATATTCGATGAATTTGAAAATATAAAACAAAATTCGGATACACGCCCAAAAATTTAACTTCTAGAAAATTTAGTAAGTATTCATTGAACTATGCATGGCCATACTTTTTAATTTTTTTTTAGAGAATAAATTTAACATAATTTAAATCAATACGCTTGAAAATCTTGTGTTTGTTTTCTTACTAAAATTGTGAGTGTTGGAATTTATGAAGTAAAATAATCGGAGCTCGATTCTTCCGGAGCGAACCTTTTGAAGGCGAAGGCGACATTCTTTCTCCAAGAGATACAGAAGGCCATGGAACCCACACCTCATCCACCGCCGCCGGCAATTTGGTCTCCGGCGCCAGCCTCTTGGGCCTCGCCCTCGGCACCTCTCGCGGCGGCGCTCCCTCCGCCCGCATCGCCGTCTACAAGATCTGCTGGTCCGATGGCTGCTTCGACGCCGACATCCTCGCAGCTTTCGACAATGCAATCGCCGATGGCGTCGACATCATCTCCATTTCCGTCGGAGGTTTTATTGCCAAGAATTACTTCAACGACTCAATCGCCATTGGGGCTTTCCACGCGATGAAGAACGGGATTCTGACTTCAAATTCCGGCGGGAATTCTGGCCCTAGCCTTGCCAGTATCACGAATGTTTCTCCATGGTCGCTATCGGTGGCGGCTAGCACTATTGATAGGAAGTTCGTGACTAATGTGAAGTTGGGTAATGGAGAAACCTTTCAGGTAAGCCCTAGATTGCTTTATTCTTTCATGAATGTGTTCTGTTTTGCGACATTCGAGTCAATGATTAACATGCACACCACCTGTTTCTTAGGGGATCTCCGTGAATACCTTCGATCTTGGAGATAAGTTGTTACCACTTATATATGCTGGTGATGCTCCTAACAAAACTGCAGGTTTTAATGAATCGTTATCAAGGTAATTGAAGCTAAAAGATTGTTTACTTATGGACTTGTTGGAAAAGTTAGGTGGCTGACTGATGTTACTCTGTAAAGATTTTATGATGAGTCTAAATGTGCAGTGTTTTTCCTAAGTTGAAGTACAAGTTTTGGGTTTGGAGGAATTGAATTTTGGTGTTAAAATGCAGGTTCTGCTTCCCTGGTTCTTTGGATGTGAACCAAGTTCAGGGGAAGATTGTTCTTTGCGATGAGATTGGTGATGGAGAAGGAGCTTTGAATAGTGGTGCAGTTGGTACGATAATGCAAGATGCTAATGACCAAGATGTTGCCTTTCTTTTCCCACTGCCTGCTTCCAAGTTAGACTTGAACACCGGAAAGAAAGTTTTTGAGTACCTGAGATCAACCAGGTATAGTGGCACATTGCTAAAAGGTTCATAGTATTTAAATACTATTTTAGTTTGTAAACTTGAGTTTTGTTTAATCTCTAAAGTTTCAAAGATTCTATTTAAGTTTTTGAACTTCATAAACTTTTTTATAAAAAGATCTTTTTAGTTTTTTCCATTTATTTATATAAATTATTTTAAAAACATCTGATATTATCCCATACTTTATTCATTTATCTTATCAATTGATGTTTACATGGAGCCAATTTGAGCATTATTCAATAGATAAGACCCCTATTACCTTCTTTGAAGTCGAGGGTTCAATCTTTGGCTCTACATTGTTGAACTTCTAAAAGAATGTCTATATGGAAAGTGGTTGTGTTTGAAGGCACATAGAGTACTTAGTAGGCTAAATTTCACGTTTCTAAATGTATATGAGTTATTTTTGAATGATTAATGAAAATTAATGGTAAGAGCTACAATCATATTTTATGTAAATCATGATGGCTAAGACAGAACATTTGAAAATTGAAAGACTTAAACCTAATATTTGAAAGTTTTAGAGTCCAAACCACGTCGATTAACTAAAATAGAATTCAAACCTTACAAATTCTTTGGGCAGTTTTGACTTTCTTAGCTTAAAATGTTTTGAAACCATCCTATTTTATTTTGTGTTTTACTTTGATAGCAATCCGGAAGCTATCATAGAAAAGAGTACTACCATTGAGGATCTGCTAGCTCCATCTGTTGTTTCCTTCTCATCTAGGGGACCTAACCTTGTTACACTGGACATTCTCAAGGTAACTCATCTGACCAAGTTTCCTCATGATTTGTTGTTAATGCCTCTTTGGATATTAACTTTTAGAAATCTCTTGTAGCCTGATTTGGCAGCACCAGGAGTGGATATACTAGCATCTTGGTCAGAAGCTTCAACAGCTACAAGTTTACCAGGGGATAAAAGAATAGTTCCATTTAACATAATCTCTGGCACATCCATGGCTTGTCCACATGCAACAGGAGTAGCTGCGTATGTCAAATCTTTCCACCCAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGGTAACAGTTTGACCTTAATTTTTTAGTACCAATAATTTAACTTAAATCATTTGTTGGTTGCTTAAACTTTCATATTCATTTCGTTTGTCTATAGACTTTTAAATATTTTGTTTCATTTGTCAATCTAAGTGTAGCTCAATTGGTTAAGGTATTATATCCTCAACCAAAAGGTCAAAGGTTTAGAATCCTCACCCCCACATGTTGTTGAATGCTAAAAAAATCTTCTGTGTCACTTGCCTTCAAAATATTATGCTTTGGTCCTTGAACTTTGATTACAAGACTTTTTTAGTCTTTATAGTTAATATTAGTCCCTAAATTTCCGACTGAAAACTATTTTAGACCTTGCAGTTAATCATTTAACAAACATTCAAATCCATTTGGAAACTTGATGCTTAATCTATTAAATATTACTTCAAACACTTGTTTTTGAAGATATCTATTGTTTAGGTAAATAAACAAAGATTTGTTAATATTAAGTTTCTATTGAGTAATATATTAAAAGATTAATGATAAAATCAAAATAGTTTTTATACAAAGTTTAGAGACTAAAACAAAATATTCGAAAGTTAATAGAGTAAAATAAAATAAGTTTAAAAGTTTTAATATTTTAGTCAAATAATGATTTTCCTGGACTTTTAATATCAGAATAAAATTAAATGTAGATGTGCCAGGGCACTCATTTATCAAATTTATCAATGTCACTGAGAATAGTAGTGTTGTAAACATTGTTCCCTATGCACTTGGCTAACCTCAATCTTTTCTCTCTTACAGCGTTTCCCATGACTCCAAAGGTAAACACAGATGCTGAGTTTGCATATGGAGCAGGACACTTAAATCCAGTAAATGCCATCAACCCCGGTTTAGTCTATGATGCCGAAGAGATTGATTACATCAAGTTCTTATGTGGGCAAGGATATAGTACCGAAAATCTTCGTCTTGTTACCGGTGACCAAAGCAACTGTTCAGTTGTTACAAAAACAGCTGCATCAGATCTAAACTATCCATCGTTTGCTCTGTTGAGCAAGTCTAAAAGATCCTTTAGTCGTGTCTACCACAGGACAGTCACGAATGTCGGGCTGCCAAGGTCGACGTATAAAGCAGTCATTCAAGCTCCACCCGGACTCAAAGTTACAGTACGTCCTGCTACTCTCTCTTTCAGTTCTCTTGGACAAAAGATATCATTCACTGTGAGGGTTAAAGCAACAGCTCCTGGTGGGAAGGTGATTTCTGGTAGTTTAACCTGGGATGATGGAGTGCATCTGGTGAGGAGCCCCATTGTTGCATTTGTTATTCCATTATGAGAGCTTCGTATGCTTATTTAGTATTGTTGGGAAGTCCCCTATATTTCCTTTATGATTCTATCAATAAACTCTTCTTCATGAGTCGTTTGGAAATCTAAATGATATTTAATATTATAAAAGAAACAATGATTTGCTTTGAGAATTCCCTTCCGACGTGAAAAGAGAACAAAA

mRNA sequence

ATGTTTTTTTTTTTTGAGTTACTAATGAGTGTGTTTGAAAGGCAGGTGTATGTTGTATACATGGGCAAATCATCTGAAGGAGGTTTCACAGCAGCATCTGAACGACATACCAATATGCTTCAACAAGTTCTTACCACCAGTGATGCATCCAAATCTCTGGTGTATAGCTACCACCGGAGCTTCAGCGGCTTTGCAGCGAGGCTCAACGAGGAGGAGGCTCGGAAACTTGCCGAAATGGAGGAAGTTGTATCTGTGTTTCCGAGCGAAAAAAAACAGCTTCACACGACAAGATCATGGGATTTCATGGGTTTATTCGAAGAAGCTCGAAGAACGAGCTTGGAGAGCGATCTGGTCGTCGGAATGTTGGACACTGGAATTTGGCCGGAATCTGAAAGCTTTTCCGATGAAGGGTTTGGTCCGCCGCCGGCGAAGTGGAAAGGCGAATGCCAACCGTCTTCCAATTTCACCTGCAACAATAAAATAATCGGAGCTCGATTCTTCCGGAGCGAACCTTTTGAAGGCGAAGGCGACATTCTTTCTCCAAGAGATACAGAAGGCCATGGAACCCACACCTCATCCACCGCCGCCGGCAATTTGGTCTCCGGCGCCAGCCTCTTGGGCCTCGCCCTCGGCACCTCTCGCGGCGGCGCTCCCTCCGCCCGCATCGCCGTCTACAAGATCTGCTGGTCCGATGGCTGCTTCGACGCCGACATCCTCGCAGCTTTCGACAATGCAATCGCCGATGGCGTCGACATCATCTCCATTTCCGTCGGAGGTTTTATTGCCAAGAATTACTTCAACGACTCAATCGCCATTGGGGCTTTCCACGCGATGAAGAACGGGATTCTGACTTCAAATTCCGGCGGGAATTCTGGCCCTAGCCTTGCCAGTATCACGAATGTTTCTCCATGGTCGCTATCGGTGGCGGCTAGCACTATTGATAGGAAGTTCGTGACTAATGTGAAGTTGGGTAATGGAGAAACCTTTCAGGGGATCTCCGTGAATACCTTCGATCTTGGAGATAAGTTGTTACCACTTATATATGCTGGTGATGCTCCTAACAAAACTGCAGGTTTTAATGAATCGTTATCAAGGTTCTGCTTCCCTGGTTCTTTGGATGTGAACCAAGTTCAGGGGAAGATTGTTCTTTGCGATGAGATTGGTGATGGAGAAGGAGCTTTGAATAGTGGTGCAGTTGGTACGATAATGCAAGATGCTAATGACCAAGATGTTGCCTTTCTTTTCCCACTGCCTGCTTCCAAGTTAGACTTGAACACCGGAAAGAAAGTTTTTGAGTACCTGAGATCAACCAGCAATCCGGAAGCTATCATAGAAAAGAGTACTACCATTGAGGATCTGCTAGCTCCATCTGTTGTTTCCTTCTCATCTAGGGGACCTAACCTTGTTACACTGGACATTCTCAAGCCTGATTTGGCAGCACCAGGAGTGGATATACTAGCATCTTGGTCAGAAGCTTCAACAGCTACAAGTTTACCAGGGGATAAAAGAATAGTTCCATTTAACATAATCTCTGGCACATCCATGGCTTGTCCACATGCAACAGGAGTAGCTGCGTATGTCAAATCTTTCCACCCAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGCGTTTCCCATGACTCCAAAGGTAAACACAGATGCTGAGTTTGCATATGGAGCAGGACACTTAAATCCAGTAAATGCCATCAACCCCGGTTTAGTCTATGATGCCGAAGAGATTGATTACATCAAGTTCTTATGTGGGCAAGGATATAGTACCGAAAATCTTCGTCTTGTTACCGGTGACCAAAGCAACTGTTCAGTTGTTACAAAAACAGCTGCATCAGATCTAAACTATCCATCGTTTGCTCTGTTGAGCAAGTCTAAAAGATCCTTTAGTCGTGTCTACCACAGGACAGTCACGAATGTCGGGCTGCCAAGGTCGACGTATAAAGCAGTCATTCAAGCTCCACCCGGACTCAAAGTTACAGTACGTCCTGCTACTCTCTCTTTCAGTTCTCTTGGACAAAAGATATCATTCACTGTGAGGGTTAAAGCAACAGCTCCTGGTGGGAAGGTGATTTCTGGTAGTTTAACCTGGGATGATGGAGTGCATCTGGTGAGGAGCCCCATTGTTGCATTTGTTATTCCATTATGA

Coding sequence (CDS)

ATGTTTTTTTTTTTTGAGTTACTAATGAGTGTGTTTGAAAGGCAGGTGTATGTTGTATACATGGGCAAATCATCTGAAGGAGGTTTCACAGCAGCATCTGAACGACATACCAATATGCTTCAACAAGTTCTTACCACCAGTGATGCATCCAAATCTCTGGTGTATAGCTACCACCGGAGCTTCAGCGGCTTTGCAGCGAGGCTCAACGAGGAGGAGGCTCGGAAACTTGCCGAAATGGAGGAAGTTGTATCTGTGTTTCCGAGCGAAAAAAAACAGCTTCACACGACAAGATCATGGGATTTCATGGGTTTATTCGAAGAAGCTCGAAGAACGAGCTTGGAGAGCGATCTGGTCGTCGGAATGTTGGACACTGGAATTTGGCCGGAATCTGAAAGCTTTTCCGATGAAGGGTTTGGTCCGCCGCCGGCGAAGTGGAAAGGCGAATGCCAACCGTCTTCCAATTTCACCTGCAACAATAAAATAATCGGAGCTCGATTCTTCCGGAGCGAACCTTTTGAAGGCGAAGGCGACATTCTTTCTCCAAGAGATACAGAAGGCCATGGAACCCACACCTCATCCACCGCCGCCGGCAATTTGGTCTCCGGCGCCAGCCTCTTGGGCCTCGCCCTCGGCACCTCTCGCGGCGGCGCTCCCTCCGCCCGCATCGCCGTCTACAAGATCTGCTGGTCCGATGGCTGCTTCGACGCCGACATCCTCGCAGCTTTCGACAATGCAATCGCCGATGGCGTCGACATCATCTCCATTTCCGTCGGAGGTTTTATTGCCAAGAATTACTTCAACGACTCAATCGCCATTGGGGCTTTCCACGCGATGAAGAACGGGATTCTGACTTCAAATTCCGGCGGGAATTCTGGCCCTAGCCTTGCCAGTATCACGAATGTTTCTCCATGGTCGCTATCGGTGGCGGCTAGCACTATTGATAGGAAGTTCGTGACTAATGTGAAGTTGGGTAATGGAGAAACCTTTCAGGGGATCTCCGTGAATACCTTCGATCTTGGAGATAAGTTGTTACCACTTATATATGCTGGTGATGCTCCTAACAAAACTGCAGGTTTTAATGAATCGTTATCAAGGTTCTGCTTCCCTGGTTCTTTGGATGTGAACCAAGTTCAGGGGAAGATTGTTCTTTGCGATGAGATTGGTGATGGAGAAGGAGCTTTGAATAGTGGTGCAGTTGGTACGATAATGCAAGATGCTAATGACCAAGATGTTGCCTTTCTTTTCCCACTGCCTGCTTCCAAGTTAGACTTGAACACCGGAAAGAAAGTTTTTGAGTACCTGAGATCAACCAGCAATCCGGAAGCTATCATAGAAAAGAGTACTACCATTGAGGATCTGCTAGCTCCATCTGTTGTTTCCTTCTCATCTAGGGGACCTAACCTTGTTACACTGGACATTCTCAAGCCTGATTTGGCAGCACCAGGAGTGGATATACTAGCATCTTGGTCAGAAGCTTCAACAGCTACAAGTTTACCAGGGGATAAAAGAATAGTTCCATTTAACATAATCTCTGGCACATCCATGGCTTGTCCACATGCAACAGGAGTAGCTGCGTATGTCAAATCTTTCCACCCAACTTGGTCTCCAGCTGCTATTAAGTCTGCACTTATGACAACAGCGTTTCCCATGACTCCAAAGGTAAACACAGATGCTGAGTTTGCATATGGAGCAGGACACTTAAATCCAGTAAATGCCATCAACCCCGGTTTAGTCTATGATGCCGAAGAGATTGATTACATCAAGTTCTTATGTGGGCAAGGATATAGTACCGAAAATCTTCGTCTTGTTACCGGTGACCAAAGCAACTGTTCAGTTGTTACAAAAACAGCTGCATCAGATCTAAACTATCCATCGTTTGCTCTGTTGAGCAAGTCTAAAAGATCCTTTAGTCGTGTCTACCACAGGACAGTCACGAATGTCGGGCTGCCAAGGTCGACGTATAAAGCAGTCATTCAAGCTCCACCCGGACTCAAAGTTACAGTACGTCCTGCTACTCTCTCTTTCAGTTCTCTTGGACAAAAGATATCATTCACTGTGAGGGTTAAAGCAACAGCTCCTGGTGGGAAGGTGATTTCTGGTAGTTTAACCTGGGATGATGGAGTGCATCTGGTGAGGAGCCCCATTGTTGCATTTGTTATTCCATTATGA

Protein sequence

MFFFFELLMSVFERQVYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPESESFSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDVNQVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPMTPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCSVVTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRPATLSFSSLGQKISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPIVAFVIPL
Homology
BLAST of Spg009742 vs. NCBI nr
Match: KAG6600966.1 (hypothetical protein SDJN03_06199, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 612/706 (86.69%), Postives = 652/706 (92.35%), Query Frame = 0

Query: 16  VYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEARK 75
           VY+VYMGK SEGGF+A S+RHTNMLQQVLT SDASKSLVYSYHRSF+GFAA LN+EEARK
Sbjct: 61  VYIVYMGKPSEGGFSAVSQRHTNMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNQEEARK 120

Query: 76  LAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPESESFSD 135
            A+M+EVVSVFPSEKKQLHTTRSWDFMG F++ARR+SLESDLV+GMLDTGIWPESESFSD
Sbjct: 121 FAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSD 180

Query: 136 EGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSSTA 195
           EGFGPPP KWKGECQP+SNFTCNNKIIGARFFRSEP  GEGDILSPRDTEGHGTHTSSTA
Sbjct: 181 EGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPL-GEGDILSPRDTEGHGTHTSSTA 240

Query: 196 AGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISI 255
            GNLVSGASL GL LGT+RGG PSARIAVYKICWSDGCFDADILAAFDNAIADGVDIIS+
Sbjct: 241 GGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISL 300

Query: 256 SVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTIDR 315
           SVGGFIAK+YFNDSIAIGAFHAMKNGILTSNSGGNSGPSL SITNVSPWSLSVAASTIDR
Sbjct: 301 SVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDR 360

Query: 316 KFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDVN 375
           KFVTNVKLGNGE+ QGISVNTF LGDKL+PLIYAGDAPN TAGFNESLSRFCFPGSLDVN
Sbjct: 361 KFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVN 420

Query: 376 QVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYLR 435
           QV+GKIVLCDEI DGE AL+SGAVGTIMQD   QD AFLFPLPAS LDLN G  VF+YLR
Sbjct: 421 QVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLR 480

Query: 436 STSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEASTA 495
           STSNPEA IEKSTTIED+ APSVVSFSSRGPNL+TLDILKPDLAAPGVDI+ASWSE +T 
Sbjct: 481 STSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTI 540

Query: 496 TSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPMTPKVN 555
             L GD R+ PFNIISGTSMACPHATG AAYVKSFHPTWSPAAIKSALMTTAFPM PK+N
Sbjct: 541 GGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLN 600

Query: 556 TDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCSVVTK 615
           TDAEFAYGAGH+NPVNAINPGLVYDAEEIDYIKFLCGQGYST+NLRLVTGD+SNCS   K
Sbjct: 601 TDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSNAAK 660

Query: 616 TAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRPATLSF 675
           TAASDLNYPSF LL KS++  +RVYHRTVTNVG P STYKAVI+APPGL+VTVRPATLSF
Sbjct: 661 TAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTVRPATLSF 720

Query: 676 SSLGQKISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPIVAFVIP 722
            SLGQK+SF+VRVKA   GG ++SGSL+W+DGVHLVRSPIVAF  P
Sbjct: 721 RSLGQKMSFSVRVKANT-GGNILSGSLSWEDGVHLVRSPIVAFATP 764

BLAST of Spg009742 vs. NCBI nr
Match: KAG7031587.1 (hypothetical protein SDJN02_05628, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 613/713 (85.97%), Postives = 653/713 (91.58%), Query Frame = 0

Query: 15  QVYVVYMGKSSEGGFTAASERHTNMLQQVLT------TSDASKSLVYSYHRSFSGFAARL 74
           QVY+VYMGK SEGGF+A S+RHTNMLQQ LT       SDASKSLVYSYHRSF+GFAA L
Sbjct: 2   QVYIVYMGKPSEGGFSAVSQRHTNMLQQGLTFQTNYDLSDASKSLVYSYHRSFNGFAAWL 61

Query: 75  NEEEARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWP 134
           N+EEARK A+M+EVVSVFPSEKKQLHTTRSWDFMG F++ARR+SLESDLV+GMLDTGIWP
Sbjct: 62  NQEEARKFAKMDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWP 121

Query: 135 ESESFSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHG 194
           ESESFSDEGFGPPP KWKGECQP+SNFTCNNKIIGARFFRSEP  GEGDILSPRDTEGHG
Sbjct: 122 ESESFSDEGFGPPPPKWKGECQPASNFTCNNKIIGARFFRSEPL-GEGDILSPRDTEGHG 181

Query: 195 THTSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIAD 254
           THTSSTA GNLVSGASL GL LGT+RGG PSARIAVYKICWSDGCFDADILAAFDNAIAD
Sbjct: 182 THTSSTAGGNLVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIAD 241

Query: 255 GVDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSV 314
           GVDIIS+SVGGFIAK+YFNDSIAIGAFHAMKNGILTSNSGGNSGPSL SITNVSPWSLSV
Sbjct: 242 GVDIISLSVGGFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSV 301

Query: 315 AASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCF 374
           AASTIDRKFVTNVKLGNGE+ QGISVNTF LGDKL+PLIYAGDAPN TAGFNESLSRFCF
Sbjct: 302 AASTIDRKFVTNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCF 361

Query: 375 PGSLDVNQVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGK 434
           PGSLDVNQV+GKIVLCDEI DGE AL+SGAVGTIMQD   QD AFLFPLPAS LDLN G 
Sbjct: 362 PGSLDVNQVEGKIVLCDEISDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGN 421

Query: 435 KVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILAS 494
            VF+YLRSTSNPEA IEKSTTIED+ APSVVSFSSRGPNL+TLDILKPDLAAPGVDI+AS
Sbjct: 422 NVFQYLRSTSNPEATIEKSTTIEDMSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVAS 481

Query: 495 WSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAF 554
           WSE +T   L GD R+ PFNIISGTSMACPHATG AAYVKSFHPTWSPAAIKSALMTTAF
Sbjct: 482 WSEGTTIGGLEGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAF 541

Query: 555 PMTPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQS 614
           PM PK+NTDAEFAYGAGH+NPVNAINPGLVYDAEEIDYIKFLCGQGYST+NLRLVTGD+S
Sbjct: 542 PMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDES 601

Query: 615 NCSVVTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTV 674
           NCS   KTAASDLNYPSF LL KS++  +RVYHRTVTNVG P STYKAVI+APPGL+VTV
Sbjct: 602 NCSNAAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGEPVSTYKAVIRAPPGLEVTV 661

Query: 675 RPATLSFSSLGQKISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPIVAFVIP 722
           RPATLSF SLGQK+SF+VRVKA   GG ++SGSL+W+DGVHLVRSPIVAF IP
Sbjct: 662 RPATLSFRSLGQKMSFSVRVKANT-GGNILSGSLSWEDGVHLVRSPIVAFAIP 712

BLAST of Spg009742 vs. NCBI nr
Match: XP_022990086.1 (cucumisin-like [Cucurbita maxima])

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 608/701 (86.73%), Postives = 650/701 (92.72%), Query Frame = 0

Query: 21  MGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEARKLAEME 80
           MGK SEGGF+A S+RHT+MLQQVLT SDASKSLVYSYHRSF+GFAA LNEEEARK A+M+
Sbjct: 1   MGKPSEGGFSAVSQRHTHMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNEEEARKFAKMD 60

Query: 81  EVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPESESFSDEGFGP 140
           EVVSVFPSEKKQLHTTRSWDFMG F++ARR+SLESDLV+GMLDTGIWPESESFSDEG GP
Sbjct: 61  EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGLGP 120

Query: 141 PPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSSTAAGNLV 200
           PP KWKG CQP+SNFTCNNKIIGARFFRSEP  GEGDILSPRDTEGHGTHT+STA GNLV
Sbjct: 121 PPPKWKGGCQPASNFTCNNKIIGARFFRSEPL-GEGDILSPRDTEGHGTHTASTAGGNLV 180

Query: 201 SGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVGGF 260
           SGASL GL LGT+RGG PSARIAVYKICWSDGCFDADILAAFDNAIADGVDIIS+SVGGF
Sbjct: 181 SGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGF 240

Query: 261 IAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTIDRKFVTN 320
           IAK+YFNDSIAIGAFHAMKNGILTSNSGGNSGPSL SITNVSPWSLSVAASTIDRKFVTN
Sbjct: 241 IAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTN 300

Query: 321 VKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDVNQVQGK 380
           VKLGNGE+ QGISVNTF LGDKL+PLIYAGDAPN TAGFNESLSRFCFPGSLDVNQV+GK
Sbjct: 301 VKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGK 360

Query: 381 IVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYLRSTSNP 440
           IVLCDEIGDGE AL+SGAVGTIMQD   QD AFLFPLPAS LDLN G  VF+YLRSTSNP
Sbjct: 361 IVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNP 420

Query: 441 EAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEASTATSLPG 500
           EA IEKSTTIEDL APSVVSFSSRGPNL+TLDILKPDLAAPGVDI+ASWSE +T   L G
Sbjct: 421 EATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEG 480

Query: 501 DKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPMTPKVNTDAEF 560
           D R+ PFNIISGTSMACPHATG AAYVKSFHPTWSPAAIKSALMTTAFPM PK+NTDAEF
Sbjct: 481 DNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEF 540

Query: 561 AYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCSVVTKTAASD 620
           AYGAGH+NP NAINPGLVYDAEEIDYIKFLCGQGYST+NLRLVTGD+SNCS V KTAASD
Sbjct: 541 AYGAGHINPANAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSDVAKTAASD 600

Query: 621 LNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRPATLSFSSLGQ 680
           LNYPSF+LL KS++  +RVYHRTVTNVG+P STYKAVI+APPGL+VTVRPATLSF SLGQ
Sbjct: 601 LNYPSFSLLMKSQKLNTRVYHRTVTNVGVPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQ 660

Query: 681 KISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPIVAFVIP 722
           K+SFTVRV+A + GG ++SGSL+W+DGVHLVRSPIVAF IP
Sbjct: 661 KMSFTVRVRANS-GGNILSGSLSWEDGVHLVRSPIVAFGIP 699

BLAST of Spg009742 vs. NCBI nr
Match: XP_022139189.1 (cucumisin-like [Momordica charantia])

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 598/712 (83.99%), Postives = 641/712 (90.03%), Query Frame = 0

Query: 13  ERQVYVVYMGKSS--EGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNE 72
           +R+VYVVYMGK S  EGGF+AAS RH NMLQQVLTTSDAS SLVYSYHRSFSGFAARLN+
Sbjct: 16  DRKVYVVYMGKPSEVEGGFSAASARHANMLQQVLTTSDASNSLVYSYHRSFSGFAARLND 75

Query: 73  EEARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPES 132
           +EARKLAEME VVSVF SEKK+LHTTRSWDFMGL +EA RT LESD++VGMLDTGIWPES
Sbjct: 76  DEARKLAEMEGVVSVFVSEKKELHTTRSWDFMGLLDEASRTRLESDVIVGMLDTGIWPES 135

Query: 133 ESFSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTH 192
           +SF+DEGFGPPP KWKG+CQ SSNFTCNNK+IGARF+RS P EG GDILSPRDTEGHGTH
Sbjct: 136 KSFTDEGFGPPPPKWKGKCQSSSNFTCNNKLIGARFYRSNPVEGGGDILSPRDTEGHGTH 195

Query: 193 TSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGV 252
           TSSTAAGNLV+ ASL GL LGTSRGG PSAR+AVYKICWSDGC DADILAAFD+AIADGV
Sbjct: 196 TSSTAAGNLVAQASLFGLGLGTSRGGVPSARVAVYKICWSDGCSDADILAAFDDAIADGV 255

Query: 253 DIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAA 312
           D+ISISVGG I + YF DSIAIGAFHAMKNGILTSNS GN+GP  A+I+NVSPWSLSVAA
Sbjct: 256 DVISISVGGSIPREYFKDSIAIGAFHAMKNGILTSNSAGNTGPGSATISNVSPWSLSVAA 315

Query: 313 STIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPG 372
           STIDRKFVT VKLGNG+ F+GISVNTF L DK+ PLIYAGDAPN TAGFN SLSRFCFPG
Sbjct: 316 STIDRKFVTKVKLGNGKAFEGISVNTFQLEDKMFPLIYAGDAPNSTAGFNGSLSRFCFPG 375

Query: 373 SLDVNQVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKV 432
           SLD+N VQGKIV CDEIGDG  AL+SGA+GTIMQDAN  DVAF+FPLPASKLDLN G KV
Sbjct: 376 SLDMNLVQGKIVFCDEIGDGTTALSSGAIGTIMQDANFLDVAFVFPLPASKLDLNAGSKV 435

Query: 433 FEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWS 492
           F+YLRSTSNPEA IEKSTTI+DL AP VVSFSSRGPN +TLDILKPDL APGVDILASWS
Sbjct: 436 FQYLRSTSNPEANIEKSTTIDDLSAPFVVSFSSRGPNQITLDILKPDLTAPGVDILASWS 495

Query: 493 EASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPM 552
           E +T TSL GD R+VPFNIISGTSM+CPHATG AAYVKSFHPTWSPAAIKSALMTTAFPM
Sbjct: 496 EDATVTSLAGDNRVVPFNIISGTSMSCPHATGTAAYVKSFHPTWSPAAIKSALMTTAFPM 555

Query: 553 TPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNC 612
           TPK+N DAEFAYGAGHLNPVNAINPGLVYDA EIDYIKFLCGQG+ST NL LVTGDQSNC
Sbjct: 556 TPKLNADAEFAYGAGHLNPVNAINPGLVYDATEIDYIKFLCGQGFSTNNLSLVTGDQSNC 615

Query: 613 SVVTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRP 672
           S V KTAASDLNYPSFAL  K++R  SRV+HRTVTNVGLP STYKA+IQA PGLKVTVRP
Sbjct: 616 SDVRKTAASDLNYPSFALSIKTRRLVSRVFHRTVTNVGLPVSTYKAIIQAAPGLKVTVRP 675

Query: 673 ATLSFSSLGQKISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPIVAFVIPL 723
           ATLSFSSLGQKISFTVR+KA    GK+ISGSLTWDDGVHLVRSPIV FV PL
Sbjct: 676 ATLSFSSLGQKISFTVRIKAKT-AGKLISGSLTWDDGVHLVRSPIVTFVNPL 726

BLAST of Spg009742 vs. NCBI nr
Match: XP_038891640.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 593/692 (85.69%), Postives = 632/692 (91.33%), Query Frame = 0

Query: 33  SERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEARKLAEMEEVVSVFPSEKKQ 92
           S+  +++  Q  TTSDASKSLVYSYHRSF+GFAARLNEEEARKLAEM+EVVSVFPSEKKQ
Sbjct: 220 SDWSSDLDDQKSTTSDASKSLVYSYHRSFNGFAARLNEEEARKLAEMDEVVSVFPSEKKQ 279

Query: 93  LHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPESESFSDEGFGPPPAKWKGECQPS 152
           LHTTRSWDFMG F++ARRTSLE+DL++GMLDTGIWPES+SFSDEGFGPPP KW G+C+P 
Sbjct: 280 LHTTRSWDFMGFFQQARRTSLETDLIIGMLDTGIWPESQSFSDEGFGPPPPKWNGKCEPV 339

Query: 153 SNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSSTAAGNLVSGASLLGLALGT 212
           SNFTCNNKIIGAR FRSEP  G  DILSPRDTEGHGTHTSSTA GN VS ASL GLA GT
Sbjct: 340 SNFTCNNKIIGARVFRSEPLFG-ADILSPRDTEGHGTHTSSTAGGNFVSDASLFGLAAGT 399

Query: 213 SRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVGGFIAKNYFNDSIAI 272
           SRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVGGF+AKNYFNDSIAI
Sbjct: 400 SRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVGGFVAKNYFNDSIAI 459

Query: 273 GAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTIDRKFVTNVKLGNGETFQGI 332
           GAFHAMKNGILTSNSGGNSGPSL +ITNVSPWSLSVAASTIDRKFVTNVKLGNGE+FQG 
Sbjct: 460 GAFHAMKNGILTSNSGGNSGPSLGTITNVSPWSLSVAASTIDRKFVTNVKLGNGESFQGT 519

Query: 333 SVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDVNQVQGKIVLCDEIGDGEG 392
           SVNTF LGDKL PLI+AG+APN TAGFN SLSRFC PGSLDV++V+GKIVLCDEIGDG  
Sbjct: 520 SVNTFQLGDKLFPLIHAGEAPNTTAGFNGSLSRFCIPGSLDVDKVRGKIVLCDEIGDGGP 579

Query: 393 ALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYLRSTSNPEAIIEKSTTIED 452
           AL+SGAVGTIMQDA  QDVAFLFPLP S +DL  GK VF+YLRS  NPEA IEKSTTIED
Sbjct: 580 ALSSGAVGTIMQDAGHQDVAFLFPLPVSLIDLKAGKNVFQYLRSNRNPEATIEKSTTIED 639

Query: 453 LLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEASTATSLPGDKRIVPFNIISG 512
           L APSVVSFSSRGPN+VTLDILKPDLAAPGVDILASWSE +T T L GD RI PFNIISG
Sbjct: 640 LSAPSVVSFSSRGPNIVTLDILKPDLAAPGVDILASWSEGTTITGLEGDNRIAPFNIISG 699

Query: 513 TSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPMTPKVNTDAEFAYGAGHLNPVNA 572
           TSMACPHATG AAYVKSFHPTWSPAAIKSALMT+AFPMTPK+N DAEFAYGAGHLNPVNA
Sbjct: 700 TSMACPHATGAAAYVKSFHPTWSPAAIKSALMTSAFPMTPKLNADAEFAYGAGHLNPVNA 759

Query: 573 INPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCSVVTKTAASDLNYPSFALL--S 632
           INPGLVYDAEE+DYIKFLCGQGYSTENLRLVTGDQSNCS VTKTAASDLNYPSF+L+  S
Sbjct: 760 INPGLVYDAEELDYIKFLCGQGYSTENLRLVTGDQSNCSGVTKTAASDLNYPSFSLVINS 819

Query: 633 KSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRPATLSFSSLGQKISFTVRVKA 692
            S+R  SRVYHRTVTNVGLP STYKAVI+APPGLKVTVRPATLSF SLGQKISFTV +K+
Sbjct: 820 PSRRLISRVYHRTVTNVGLPVSTYKAVIKAPPGLKVTVRPATLSFRSLGQKISFTVTIKS 879

Query: 693 TAP-GGKVISGSLTWDDGVHLVRSPIVAFVIP 722
            A  GGK++SGSLTWDDGVHLVRSPIV+FVIP
Sbjct: 880 KAEVGGKMLSGSLTWDDGVHLVRSPIVSFVIP 910

BLAST of Spg009742 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 8.7e-216
Identity = 387/704 (54.97%), Postives = 505/704 (71.73%), Query Frame = 0

Query: 14  RQVYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEA 73
           + +Y+VYMG+  E    +A   H  ML+QV+ ++ A +S++++Y RSF+GFA +L EEEA
Sbjct: 31  KNIYIVYMGRKLEDP-DSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEA 90

Query: 74  RKLAEMEEVVSVFPSEKKQLHTTRSWDFMGL-FEEARRTSLESDLVVGMLDTGIWPESES 133
            K+A ME VVSVF +E  +LHTTRSWDF+G      RR+ +ES++VVG+LDTGIWPES S
Sbjct: 91  EKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPS 150

Query: 134 FSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTS 193
           F DEGF PPP KWKG C+ S+NF CN KIIGAR +        GD+  PRDT GHGTHT+
Sbjct: 151 FDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTA 210

Query: 194 STAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDI 253
           STAAG LVS A+L GL LGT+RGG P ARIA YK+CW+DGC D DILAA+D+AIADGVDI
Sbjct: 211 STAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDI 270

Query: 254 ISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAAST 313
           IS+SVGG   ++YF D+IAIG+FHA++ GILTSNS GN GP+  +  ++SPW LSVAAST
Sbjct: 271 ISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAAST 330

Query: 314 IDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSL 373
           +DRKFVT V++GNG++FQG+S+NTFD  ++  PL+   D PN   GF++S SRFC   S+
Sbjct: 331 MDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPN--TGFDKSTSRFCTDKSV 390

Query: 374 DVNQVQGKIVLCD-EIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVF 433
           + N ++GKIV+C+   G  E   +      ++  +N +D A  +PLP+S LD N      
Sbjct: 391 NPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLATL 450

Query: 434 EYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSE 493
            Y+ S  +P A I KSTTI +  AP VVSFSSRGPN  T D++KPD++ PGV+ILA+W  
Sbjct: 451 RYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAW-- 510

Query: 494 ASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPMT 553
             +   + G +R   FNIISGTSM+CPH TG+A YVK+++PTWSPAAIKSALMTTA PM 
Sbjct: 511 -PSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMN 570

Query: 554 PKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCS 613
            + N  AEFAYG+GH+NP+ A+ PGLVYDA E DY+KFLCGQGY+T+ +R +TGD S C+
Sbjct: 571 ARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACT 630

Query: 614 VVTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRPA 673
                   DLNYPSF L     ++F++ ++RT+T+V    STY+A+I AP GL ++V P 
Sbjct: 631 SGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPN 690

Query: 674 TLSFSSLGQKISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPI 716
            LSF+ LG + SFT+ V+ +   G V+S SL W DGVH VRSPI
Sbjct: 691 VLSFNGLGDRKSFTLTVRGSIK-GFVVSASLVWSDGVHYVRSPI 725

BLAST of Spg009742 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.8e-192
Identity = 363/710 (51.13%), Postives = 473/710 (66.62%), Query Frame = 0

Query: 16  VYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEARK 75
           VY+VYMG   E  ++  S  H ++LQ+++ T  AS  LV SY RSF+GFAA L++ E++K
Sbjct: 32  VYIVYMGTLPEIKYSPPS-HHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQK 91

Query: 76  LAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSL-ESDLVVGMLDTGIWPESESFS 135
           L  M+EVVSVFPS+  +L TTRSWDF+G  E+ARR S+ ESD++VG++D+GIWPESESF 
Sbjct: 92  LQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFD 151

Query: 136 DEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSST 195
           DEGFGPPP KWKG C+    F CNNK+IGARF+    F       S RD EGHGTHT+ST
Sbjct: 152 DEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNK--FAD-----SARDEEGHGTHTAST 211

Query: 196 AAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIIS 255
           AAGN V  AS  GLA GT+RGG PSARIA YK+C+ + C D DILAAFD+AIADGVD+IS
Sbjct: 212 AAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVIS 271

Query: 256 ISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTID 315
           IS+      N  N S+AIG+FHAM  GI+T+ S GN+GP   S+ NVSPW ++VAAS  D
Sbjct: 272 ISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTD 331

Query: 316 RKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDV 375
           R+F+  V LGNG+   GISVNTF+L     P++Y     N +   +++ + +C  G +D 
Sbjct: 332 RQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDS 391

Query: 376 NQVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYL 435
             V+GKIVLCD+      A  +GA+G I+Q+    D AF+ P PAS L     K +  Y+
Sbjct: 392 ELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYI 451

Query: 436 RSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEAST 495
            S   P+A I ++  I D  AP V SFSSRGP+ V  ++LKPD++APG++ILA++S  ++
Sbjct: 452 ESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVAS 511

Query: 496 ATSL--PGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPMTP 555
            +S   P DKR V ++++SGTSMACPH  GVAAYVKSFHP WSP+AIKSA+MTTA PM  
Sbjct: 512 PSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNL 571

Query: 556 KVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCSV 615
           K N + EFAYG+G +NP  A +PGLVY+ E  DY+K LC +G+ +  L   +G    CS 
Sbjct: 572 KKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS- 631

Query: 616 VTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKA-VIQAPPGLKVTVRPA 675
             +T   DLNYP+      S   F+  + RTVTNVG P STYKA V+   P L++++ P 
Sbjct: 632 -ERTEVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPE 691

Query: 676 TLSFSSLGQKISFTVRVKA-TAPGGKVISGSLTWDDGVHLVRSPIVAFVI 721
            L F  L +K SF V +       G  +S S+ W DG H VRSPIVA+ I
Sbjct: 692 ILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727

BLAST of Spg009742 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 8.0e-185
Identity = 352/715 (49.23%), Postives = 484/715 (67.69%), Query Frame = 0

Query: 14  RQVYVVYMGKSSEGGFTAASERHTNMLQQV-LTTSDASKSLVYSYHRSFSGFAARLNEEE 73
           +  Y++Y+G   +       + H N+L  + ++  +A +  VYSY ++F+ FAA+L+  E
Sbjct: 35  KDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKERKVYSYTKAFNAFAAKLSPHE 94

Query: 74  ARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEEARR-TSLESDLVVGMLDTGIWPESE 133
           A+K+ EMEEVVSV  ++ ++LHTT+SWDF+GL   A+R    E D+++G+LDTGI P+SE
Sbjct: 95  AKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSE 154

Query: 134 SFSDEGFGPPPAKWKGECQPSSNFT-CNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTH 193
           SF D G GPPPAKWKG C P  NFT CNNKIIGA++F+ +     G++ SP D +GHGTH
Sbjct: 155 SFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTH 214

Query: 194 TSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWS-DGCFDADILAAFDNAIADG 253
           TSST AG LV+ ASL G+A GT+RG  PSAR+A+YK+CW+  GC D DILA F+ AI DG
Sbjct: 215 TSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDG 274

Query: 254 VDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVA 313
           V+IISIS+GG IA +Y +DSI++G+FHAM+ GILT  S GN GPS  ++TN  PW L+VA
Sbjct: 275 VEIISISIGGPIA-DYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVA 334

Query: 314 ASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFP 373
           AS IDR F + + LGNG++F G+ ++ F    K  PL+   DA   T   ++ L+R+CF 
Sbjct: 335 ASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTD--DKYLARYCFS 394

Query: 374 GSLDVNQVQGKIVLCDEIGDG-EGALNS-GAVGTIMQDANDQDVAFLFPLPASKLDLNTG 433
            SLD  +V+GK+++C   G G E  + S G  G I+      D A +F  PA+ ++ + G
Sbjct: 395 DSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVG 454

Query: 434 KKVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILA 493
             ++ Y+ ST +  A+I+K+  +  + AP V SFSSRGPN  ++ +LKPD+AAPG+DILA
Sbjct: 455 DIIYRYINSTRSASAVIQKTRQV-TIPAPFVASFSSRGPNPGSIRLLKPDIAAPGIDILA 514

Query: 494 SWSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTA 553
           +++   + T L GD +   F I+SGTSMACPH  GVAAYVKSFHP W+PAAIKSA++T+A
Sbjct: 515 AFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKSFHPDWTPAAIKSAIITSA 574

Query: 554 FPMTPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQ 613
            P++ +VN DAEFAYG G +NP  A +PGLVYD ++I Y++FLCG+GY+   L  + G +
Sbjct: 575 KPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYNATTLAPLVGTR 634

Query: 614 S-NCSVVTKTAASD-LNYPSFAL-LSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGL 673
           S +CS +      D LNYP+  L L  +K S   V+ R VTNVG P S Y A ++AP G+
Sbjct: 635 SVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTNVGPPSSVYTATVRAPKGV 694

Query: 674 KVTVRPATLSFSSLGQKISFTVRVKA-TAPGGKVISGSLTWDDGVHLVRSPIVAF 719
           ++TV P +LSFS   QK SF V VKA     GK++SG L W    H VRSPIV +
Sbjct: 695 EITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWKSPRHSVRSPIVIY 744

BLAST of Spg009742 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 6.7e-184
Identity = 372/739 (50.34%), Postives = 493/739 (66.71%), Query Frame = 0

Query: 13  ERQVYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSL-VYSYHRSFSGFAARLNEE 72
           ER+ Y+VYMG+++E     A+E H N+L  V+     ++ L +YSY ++ +GF ARL   
Sbjct: 31  ERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESKARELKIYSYGKNINGFVARLFPH 90

Query: 73  EARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEE--ARRTSLESDLVVGMLDTGIWPE 132
           EA KL+  E VVSVF + ++QLHTTRSWDF+GL E    R   +ES+++VG+LDTGI  E
Sbjct: 91  EAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVGIESNIIVGVLDTGIDVE 150

Query: 133 SESFSDEGFGPPPAKWKGECQPSSNFT-CNNKIIGARFF--RSEPF-EGEGDILSPRDTE 192
           S SF+D+G GPPPAKWKG+C   +NFT CNNK+IGA++F  +SE   +GEGD  +  D +
Sbjct: 151 SPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEGD--TAADHD 210

Query: 193 GHGTHTSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNA 252
           GHGTHTSST AG  VS ASL G+A GT+RGG PSARIA YK+CW  GC D D+LAAFD A
Sbjct: 211 GHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDMLAAFDEA 270

Query: 253 IADGVDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWS 312
           I+DGVDIISIS+GG  +  +F D IAIGAFHAMK GILT+ S GN+GP L +++N++PW 
Sbjct: 271 ISDGVDIISISIGG-ASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWV 330

Query: 313 LSVAASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSR 372
           ++VAA+++DRKF T VKLGNG T  GIS+N F+   K+ PL     A N +AG     S 
Sbjct: 331 MTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGSLASNLSAGGYGEPST 390

Query: 373 FCFPGSLDVNQVQGKIVLCDEIGDGEGALNSG-----------AVGTIMQDANDQDVAFL 432
            C PG+L  ++V GK+V C E G  EG  N G             G I+Q     D+A  
Sbjct: 391 -CEPGTLGEDKVMGKVVYC-EAGREEGG-NGGQGQDHVVRSLKGAGVIVQLLEPTDMATS 450

Query: 433 FPLPASKLDLNTGKKVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDIL 492
             +  S +    G K+ EY+ ST NP+A+I K+ T + +LAPS+ SFS+RGP  ++ +IL
Sbjct: 451 TLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTK-MLAPSISSFSARGPQRISPNIL 510

Query: 493 KPDLAAPGVDILASWSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTW 552
           KPD++APG++ILA++S+ ++ T  P D R   F+I+SGTSMACPHA   AAYVKSFHP W
Sbjct: 511 KPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACPHAAAAAAYVKSFHPDW 570

Query: 553 SPAAIKSALMTTAFPMTPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQG 612
           SPAAIKSALMTTA PM  K N +AE +YG+G +NP  AI+PGLVYD  E  Y++FLC +G
Sbjct: 571 SPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPGLVYDITEDAYLRFLCKEG 630

Query: 613 YSTENLRLVTGDQS--------NCSVVTKTAASD-LNYPSF-ALLSKSKRSFSRVYHRTV 672
           Y++ ++ L+TGD S        NC  + +   SD LNYPS    ++ ++   S V++RTV
Sbjct: 631 YNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQVNSTEAKVSEVFYRTV 690

Query: 673 TNVGLPRSTYKAVIQAPPGLKVTVRPATLSFSSLGQKISFTVRVKA----TAPGGKVISG 719
           TNVG   STY A + AP GL+V V P  +SF    +K +F V +      T  G  ++S 
Sbjct: 691 TNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVIDGVWDETMKG--IVSA 750

BLAST of Spg009742 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 3.7e-182
Identity = 359/716 (50.14%), Postives = 470/716 (65.64%), Query Frame = 0

Query: 13  ERQVYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEE 72
           ++Q Y+VYMG             HT++LQ V   S     LV +Y RSF+GFAARL + E
Sbjct: 31  DKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSIEDRLVRNYKRSFNGFAARLTKSE 90

Query: 73  ARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEE---ARRTSLESDLVVGMLDTGIWPE 132
              LA M+EVVSVFP++K +L TT SW+FMGL E     R T +ESD ++G++D+GI+PE
Sbjct: 91  REILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKRNTIIESDTIIGVIDSGIYPE 150

Query: 133 SESFSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGT 192
           S+SFS +GFGPPP KWKG C+   NFT NNK+IGAR++  +    EG   S RD  GHG+
Sbjct: 151 SDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTPKL---EGFPESARDYMGHGS 210

Query: 193 HTSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKIC--WSDGCFDADILAAFDNAIA 252
           HT+STAAGN V   S  GL  GT+RGG P+ARIAVYK+C    DGC    ILAAFD+AIA
Sbjct: 211 HTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGVDGCTTDGILAAFDDAIA 270

Query: 253 DGVDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLS 312
           D VDII+IS+GG  +  +  D IAIGAFHAM  GIL  NS GNSGP  +++ +++PW  +
Sbjct: 271 DKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGNSGPEPSTVASIAPWMFT 330

Query: 313 VAASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFC 372
           VAAS  +R FVT V LGNG+T  G SVN+FDL  K  PL+Y   A   ++    + + FC
Sbjct: 331 VAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGKSA---SSSCGAASAGFC 390

Query: 373 FPGSLDVNQVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTG 432
            PG LD  +V+GKIVLCD   + + A   GA+ +I++ ++  DVA +F  P S L  +  
Sbjct: 391 SPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVR-SHRTDVASIFSFPVSVLLEDDY 450

Query: 433 KKVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILA 492
             V  Y+ ST NP+A + KS TI +  AP V S+ SRGPN +  DILKPD+ APG +I+A
Sbjct: 451 NTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDILKPDITAPGSEIVA 510

Query: 493 SWSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTA 552
           ++S    A     D R V +++ +GTSM+CPH  GVAAY+KSFHP WSP+ I+SA+MTTA
Sbjct: 511 AYS--PDAPPSISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTA 570

Query: 553 FPM---TPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVT 612
           +PM   T   N  AEFAYGAGH++P+ AI+PGLVY+A + D+I FLCG  Y+ +NLRL++
Sbjct: 571 WPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLIS 630

Query: 613 GDQSNCS-VVTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPG 672
           GD S+C+   TK+   +LNYPS      + + F  ++ RTVTNVG P +TYKA +     
Sbjct: 631 GDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKVVGSK- 690

Query: 673 LKVTVRPATLSFSSLGQKISFTVRVKATAPGGK-VISGSLTWDDGVHLVRSPIVAF 719
           LKV V PA LS  SL +K SFTV      P  + ++S  L W DGVH VRSPIV +
Sbjct: 691 LKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Spg009742 vs. ExPASy TrEMBL
Match: A0A6J1JLZ2 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111487088 PE=3 SV=1)

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 608/701 (86.73%), Postives = 650/701 (92.72%), Query Frame = 0

Query: 21  MGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEARKLAEME 80
           MGK SEGGF+A S+RHT+MLQQVLT SDASKSLVYSYHRSF+GFAA LNEEEARK A+M+
Sbjct: 1   MGKPSEGGFSAVSQRHTHMLQQVLTNSDASKSLVYSYHRSFNGFAAWLNEEEARKFAKMD 60

Query: 81  EVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPESESFSDEGFGP 140
           EVVSVFPSEKKQLHTTRSWDFMG F++ARR+SLESDLV+GMLDTGIWPESESFSDEG GP
Sbjct: 61  EVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGLGP 120

Query: 141 PPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSSTAAGNLV 200
           PP KWKG CQP+SNFTCNNKIIGARFFRSEP  GEGDILSPRDTEGHGTHT+STA GNLV
Sbjct: 121 PPPKWKGGCQPASNFTCNNKIIGARFFRSEPL-GEGDILSPRDTEGHGTHTASTAGGNLV 180

Query: 201 SGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVGGF 260
           SGASL GL LGT+RGG PSARIAVYKICWSDGCFDADILAAFDNAIADGVDIIS+SVGGF
Sbjct: 181 SGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVGGF 240

Query: 261 IAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTIDRKFVTN 320
           IAK+YFNDSIAIGAFHAMKNGILTSNSGGNSGPSL SITNVSPWSLSVAASTIDRKFVTN
Sbjct: 241 IAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFVTN 300

Query: 321 VKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDVNQVQGK 380
           VKLGNGE+ QGISVNTF LGDKL+PLIYAGDAPN TAGFNESLSRFCFPGSLDVNQV+GK
Sbjct: 301 VKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNESLSRFCFPGSLDVNQVEGK 360

Query: 381 IVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYLRSTSNP 440
           IVLCDEIGDGE AL+SGAVGTIMQD   QD AFLFPLPAS LDLN G  VF+YLRSTSNP
Sbjct: 361 IVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTSNP 420

Query: 441 EAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEASTATSLPG 500
           EA IEKSTTIEDL APSVVSFSSRGPNL+TLDILKPDLAAPGVDI+ASWSE +T   L G
Sbjct: 421 EATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGLEG 480

Query: 501 DKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPMTPKVNTDAEF 560
           D R+ PFNIISGTSMACPHATG AAYVKSFHPTWSPAAIKSALMTTAFPM PK+NTDAEF
Sbjct: 481 DNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTAFPMAPKLNTDAEF 540

Query: 561 AYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCSVVTKTAASD 620
           AYGAGH+NP NAINPGLVYDAEEIDYIKFLCGQGYST+NLRLVTGD+SNCS V KTAASD
Sbjct: 541 AYGAGHINPANAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDESNCSDVAKTAASD 600

Query: 621 LNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRPATLSFSSLGQ 680
           LNYPSF+LL KS++  +RVYHRTVTNVG+P STYKAVI+APPGL+VTVRPATLSF SLGQ
Sbjct: 601 LNYPSFSLLMKSQKLNTRVYHRTVTNVGVPVSTYKAVIRAPPGLEVTVRPATLSFRSLGQ 660

Query: 681 KISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPIVAFVIP 722
           K+SFTVRV+A + GG ++SGSL+W+DGVHLVRSPIVAF IP
Sbjct: 661 KMSFTVRVRANS-GGNILSGSLSWEDGVHLVRSPIVAFGIP 699

BLAST of Spg009742 vs. ExPASy TrEMBL
Match: A0A6J1CC81 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010157 PE=3 SV=1)

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 598/712 (83.99%), Postives = 641/712 (90.03%), Query Frame = 0

Query: 13  ERQVYVVYMGKSS--EGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNE 72
           +R+VYVVYMGK S  EGGF+AAS RH NMLQQVLTTSDAS SLVYSYHRSFSGFAARLN+
Sbjct: 16  DRKVYVVYMGKPSEVEGGFSAASARHANMLQQVLTTSDASNSLVYSYHRSFSGFAARLND 75

Query: 73  EEARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPES 132
           +EARKLAEME VVSVF SEKK+LHTTRSWDFMGL +EA RT LESD++VGMLDTGIWPES
Sbjct: 76  DEARKLAEMEGVVSVFVSEKKELHTTRSWDFMGLLDEASRTRLESDVIVGMLDTGIWPES 135

Query: 133 ESFSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTH 192
           +SF+DEGFGPPP KWKG+CQ SSNFTCNNK+IGARF+RS P EG GDILSPRDTEGHGTH
Sbjct: 136 KSFTDEGFGPPPPKWKGKCQSSSNFTCNNKLIGARFYRSNPVEGGGDILSPRDTEGHGTH 195

Query: 193 TSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGV 252
           TSSTAAGNLV+ ASL GL LGTSRGG PSAR+AVYKICWSDGC DADILAAFD+AIADGV
Sbjct: 196 TSSTAAGNLVAQASLFGLGLGTSRGGVPSARVAVYKICWSDGCSDADILAAFDDAIADGV 255

Query: 253 DIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAA 312
           D+ISISVGG I + YF DSIAIGAFHAMKNGILTSNS GN+GP  A+I+NVSPWSLSVAA
Sbjct: 256 DVISISVGGSIPREYFKDSIAIGAFHAMKNGILTSNSAGNTGPGSATISNVSPWSLSVAA 315

Query: 313 STIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPG 372
           STIDRKFVT VKLGNG+ F+GISVNTF L DK+ PLIYAGDAPN TAGFN SLSRFCFPG
Sbjct: 316 STIDRKFVTKVKLGNGKAFEGISVNTFQLEDKMFPLIYAGDAPNSTAGFNGSLSRFCFPG 375

Query: 373 SLDVNQVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKV 432
           SLD+N VQGKIV CDEIGDG  AL+SGA+GTIMQDAN  DVAF+FPLPASKLDLN G KV
Sbjct: 376 SLDMNLVQGKIVFCDEIGDGTTALSSGAIGTIMQDANFLDVAFVFPLPASKLDLNAGSKV 435

Query: 433 FEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWS 492
           F+YLRSTSNPEA IEKSTTI+DL AP VVSFSSRGPN +TLDILKPDL APGVDILASWS
Sbjct: 436 FQYLRSTSNPEANIEKSTTIDDLSAPFVVSFSSRGPNQITLDILKPDLTAPGVDILASWS 495

Query: 493 EASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPM 552
           E +T TSL GD R+VPFNIISGTSM+CPHATG AAYVKSFHPTWSPAAIKSALMTTAFPM
Sbjct: 496 EDATVTSLAGDNRVVPFNIISGTSMSCPHATGTAAYVKSFHPTWSPAAIKSALMTTAFPM 555

Query: 553 TPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNC 612
           TPK+N DAEFAYGAGHLNPVNAINPGLVYDA EIDYIKFLCGQG+ST NL LVTGDQSNC
Sbjct: 556 TPKLNADAEFAYGAGHLNPVNAINPGLVYDATEIDYIKFLCGQGFSTNNLSLVTGDQSNC 615

Query: 613 SVVTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRP 672
           S V KTAASDLNYPSFAL  K++R  SRV+HRTVTNVGLP STYKA+IQA PGLKVTVRP
Sbjct: 616 SDVRKTAASDLNYPSFALSIKTRRLVSRVFHRTVTNVGLPVSTYKAIIQAAPGLKVTVRP 675

Query: 673 ATLSFSSLGQKISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPIVAFVIPL 723
           ATLSFSSLGQKISFTVR+KA    GK+ISGSLTWDDGVHLVRSPIV FV PL
Sbjct: 676 ATLSFSSLGQKISFTVRIKAKT-AGKLISGSLTWDDGVHLVRSPIVTFVNPL 726

BLAST of Spg009742 vs. ExPASy TrEMBL
Match: A0A5A7UDF9 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001080 PE=3 SV=1)

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 576/706 (81.59%), Postives = 628/706 (88.95%), Query Frame = 0

Query: 21  MGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEARKLAEME 80
           MGK S GG  AASE HTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLN++EARKLAEM+
Sbjct: 1   MGKPSGGGLLAASELHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNDDEARKLAEMD 60

Query: 81  EVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPESESFSDEGFGP 140
            VVSVFPSEKKQLHTTRSWDFMG F+EA R  LESDL++GMLDTGIWPES+SFSDEGFGP
Sbjct: 61  GVVSVFPSEKKQLHTTRSWDFMGFFQEAPRKRLESDLIIGMLDTGIWPESKSFSDEGFGP 120

Query: 141 PPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSSTAAGNLV 200
           PP+KWKGEC  + NFTCNNKIIGARFFRSEP    GD  SPRDTEGHGTHTSSTA GNLV
Sbjct: 121 PPSKWKGEC--AHNFTCNNKIIGARFFRSEPV--IGDSPSPRDTEGHGTHTSSTAGGNLV 180

Query: 201 SGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVGGF 260
           + A+L GLA GTSRGGAPSARIAVYKICWS+GC DADILAAFD+AIADGVDIISISVGGF
Sbjct: 181 TDANLFGLAAGTSRGGAPSARIAVYKICWSNGCPDADILAAFDHAIADGVDIISISVGGF 240

Query: 261 IAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTIDRKFVTN 320
            A NY +D IAIGAFHAMKNGILTSNSGGNSGP+L S++NVSPWSLSVAASTIDRKFVT 
Sbjct: 241 GASNYLDDPIAIGAFHAMKNGILTSNSGGNSGPNLGSLSNVSPWSLSVAASTIDRKFVTK 300

Query: 321 VKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDVNQVQGK 380
           V LGNGE+ QGISVNTF+LGDKL PLI+AGDAPN TAGFN S SR CFPGSLDV++VQGK
Sbjct: 301 VTLGNGESIQGISVNTFELGDKLFPLIHAGDAPNTTAGFNGSTSRLCFPGSLDVDKVQGK 360

Query: 381 IVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYLRSTSNP 440
           IV+CD I  G+   +SGAVGT+M D+  QDVAFLFP P S +  +TGK +F+YLRS SNP
Sbjct: 361 IVICDLISGGDVTQSSGAVGTVMLDSGFQDVAFLFPQPVSLISFHTGKSLFQYLRSNSNP 420

Query: 441 EAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEASTATSLPG 500
           EAIIEKSTTIEDL APSVVSFSSRGPN++TLDILKPDLAAPGVDI+ASWSEA++ T + G
Sbjct: 421 EAIIEKSTTIEDLSAPSVVSFSSRGPNVITLDILKPDLAAPGVDIIASWSEATSITGVEG 480

Query: 501 DKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTT--AFPMTPKVNTDA 560
           DKRI PFNIISGTSM+CPHATG AAYVKSFHP+WSPAAIKSALMT+   FPM+PK+NTDA
Sbjct: 481 DKRIAPFNIISGTSMSCPHATGAAAYVKSFHPSWSPAAIKSALMTSGNTFPMSPKLNTDA 540

Query: 561 EFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCSVVTKTAA 620
           E AYGAGHLNPVNAINPGLVYDAEE+DYIKFLCGQGYST++LRLV+GD SNCS VTKTAA
Sbjct: 541 ELAYGAGHLNPVNAINPGLVYDAEELDYIKFLCGQGYSTKDLRLVSGDHSNCSDVTKTAA 600

Query: 621 SDLNYPSFALL--SKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVTVRPATLSFS 680
           SDLNYPSF L+  S S+R  SRVYHRTVTNVGLP STYKAVI+APPGLKVTVRPATLSF 
Sbjct: 601 SDLNYPSFGLVINSTSQRLISRVYHRTVTNVGLPVSTYKAVIKAPPGLKVTVRPATLSFR 660

Query: 681 SLGQKISFTVRVKATAP-GGKVISGSLTWDDGVHLVRSPIVAFVIP 722
           SLGQKISFTV V+A A   GKV+SGSLTWDDGVHLVRSPIV+FVIP
Sbjct: 661 SLGQKISFTVTVRAKADVAGKVVSGSLTWDDGVHLVRSPIVSFVIP 702

BLAST of Spg009742 vs. ExPASy TrEMBL
Match: A0A6J1GXX0 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458206 PE=3 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 562/654 (85.93%), Postives = 596/654 (91.13%), Query Frame = 0

Query: 79  MEEVVSVFPSEKKQLHTTRSWDFMGLFEEARRTSLESDLVVGMLDTGIWPESESFSDEGF 138
           M+EVVSVFPSEKKQLHTTRSWDFMG F++ARR+SLESDLV+GMLDTGIWPESESFSDEGF
Sbjct: 1   MDEVVSVFPSEKKQLHTTRSWDFMGFFQQARRSSLESDLVIGMLDTGIWPESESFSDEGF 60

Query: 139 GPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSSTAAGN 198
           GPPP KWKGECQP+SNFTCNNKIIGARFFR+EP  GEGDILSPRDTEGHGTHTSSTA GN
Sbjct: 61  GPPPPKWKGECQPASNFTCNNKIIGARFFRTEPL-GEGDILSPRDTEGHGTHTSSTAGGN 120

Query: 199 LVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVG 258
           LVSGASL GL LGT+RGG PSARIAVYKICWSDGCFDADILAAFDNAIADGVDIIS+SVG
Sbjct: 121 LVSGASLFGLGLGTARGGTPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISLSVG 180

Query: 259 GFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTIDRKFV 318
           GFIAK+YFNDSIAIGAFHAMKNGILTSNSGGNSGPSL SITNVSPWSLSVAASTIDRKFV
Sbjct: 181 GFIAKDYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLGSITNVSPWSLSVAASTIDRKFV 240

Query: 319 TNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDVNQVQ 378
           TNVKLGNGE+ QGISVNTF LGDKL+PLIYAGDAPN TAGFN SLSRFCFPGSLDVNQV+
Sbjct: 241 TNVKLGNGESSQGISVNTFQLGDKLIPLIYAGDAPNTTAGFNGSLSRFCFPGSLDVNQVE 300

Query: 379 GKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYLRSTS 438
           GKIVLCDEIGDGE AL+SGAVGTIMQD   QD AFLFPLPAS LDLN G  VF+YLRSTS
Sbjct: 301 GKIVLCDEIGDGEAALSSGAVGTIMQDGGLQDAAFLFPLPASVLDLNAGNNVFQYLRSTS 360

Query: 439 NPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEASTATSL 498
           NPEA IEKSTTIEDL APSVVSFSSRGPNL+TLDILKPDLAAPGVDI+ASWSE +T   L
Sbjct: 361 NPEATIEKSTTIEDLSAPSVVSFSSRGPNLITLDILKPDLAAPGVDIVASWSEGTTIGGL 420

Query: 499 PGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMT-----------TA 558
            GD R+ PFNIISGTSMACPHATG AAYVKSFHPTWSPAAIKSALMT           TA
Sbjct: 421 EGDNRVSPFNIISGTSMACPHATGAAAYVKSFHPTWSPAAIKSALMTTGNSLSLIVYVTA 480

Query: 559 FPMTPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQ 618
           FPM PK+NTDAEFAYGAGH+NPVNAINPGLVYDAEEIDYIKFLCGQGYST+NLRLVTGD+
Sbjct: 481 FPMAPKLNTDAEFAYGAGHINPVNAINPGLVYDAEEIDYIKFLCGQGYSTQNLRLVTGDE 540

Query: 619 SNCSVVTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKVT 678
           SNCS V KTAASDLNYPSF LL KS++  +RVYHRTVTNVG+P STYKAVI+APPGL+VT
Sbjct: 541 SNCSNVAKTAASDLNYPSFGLLMKSQKLNTRVYHRTVTNVGVPVSTYKAVIRAPPGLEVT 600

Query: 679 VRPATLSFSSLGQKISFTVRVKATAPGGKVISGSLTWDDGVHLVRSPIVAFVIP 722
           VRPATLSF SLGQK+SFTVRVKA   GG ++SGSL+W+DGVH VRSPIVAF IP
Sbjct: 601 VRPATLSFRSLGQKMSFTVRVKANT-GGNILSGSLSWEDGVHQVRSPIVAFAIP 652

BLAST of Spg009742 vs. ExPASy TrEMBL
Match: A0A1S3CMK1 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103502667 PE=3 SV=1)

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 556/678 (82.01%), Postives = 608/678 (89.68%), Query Frame = 0

Query: 47  SDASKSLVYSYHRSFSGFAARLNEEEARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFE 106
           SDASKSLVYSYHRSFSGFAARLN++EARKLAEM+ VVSVFPSEKKQLHTTRSWDFMG F+
Sbjct: 11  SDASKSLVYSYHRSFSGFAARLNDDEARKLAEMDGVVSVFPSEKKQLHTTRSWDFMGFFQ 70

Query: 107 EARRTSLESDLVVGMLDTGIWPESESFSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARF 166
           EA R  LESDL++GMLDTGIWPES+SFSDEGFGPPP+KWKGEC  + NFTCNNKIIGARF
Sbjct: 71  EAPRKRLESDLIIGMLDTGIWPESKSFSDEGFGPPPSKWKGEC--AHNFTCNNKIIGARF 130

Query: 167 FRSEPFEGEGDILSPRDTEGHGTHTSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYK 226
           FRSEP    GD  SPRDTEGHGTHTSSTA GNLV+ A+L GLA GTSRGGAPSARIAVYK
Sbjct: 131 FRSEPV--IGDSPSPRDTEGHGTHTSSTAGGNLVTDANLFGLAAGTSRGGAPSARIAVYK 190

Query: 227 ICWSDGCFDADILAAFDNAIADGVDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSN 286
           ICWS+GC DADILAAFD+AIADGVDIISISVGGF A NY +D IAIGAFHAMKNGILTSN
Sbjct: 191 ICWSNGCPDADILAAFDHAIADGVDIISISVGGFGASNYLDDPIAIGAFHAMKNGILTSN 250

Query: 287 SGGNSGPSLASITNVSPWSLSVAASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPL 346
           SGGNSGP+L S++NVSPWSLSVAASTIDRKFVT V LGNGE+ QGISVNTF+LGDKL PL
Sbjct: 251 SGGNSGPNLGSLSNVSPWSLSVAASTIDRKFVTKVTLGNGESIQGISVNTFELGDKLFPL 310

Query: 347 IYAGDAPNKTAGFNESLSRFCFPGSLDVNQVQGKIVLCDEIGDGEGALNSGAVGTIMQDA 406
           I+AGDAPN TAGFN S SR CFPGSLDV++VQGKIV+CD I  G+   +SGAVGT+M D+
Sbjct: 311 IHAGDAPNTTAGFNGSTSRLCFPGSLDVDKVQGKIVICDLISGGDVTQSSGAVGTVMLDS 370

Query: 407 NDQDVAFLFPLPASKLDLNTGKKVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGP 466
             QDVAFLFP P S +  +TGK +F+YLRS SNPEAIIEKSTTIEDL APSVVSFSSRGP
Sbjct: 371 GFQDVAFLFPQPVSLISFHTGKSLFQYLRSNSNPEAIIEKSTTIEDLSAPSVVSFSSRGP 430

Query: 467 NLVTLDILKPDLAAPGVDILASWSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAY 526
           N++TLDILKPDLAAPGVDI+ASWSEA++ T + GDKRI PFNIISGTSM+CPHATG AAY
Sbjct: 431 NVITLDILKPDLAAPGVDIIASWSEATSITGVEGDKRIAPFNIISGTSMSCPHATGAAAY 490

Query: 527 VKSFHPTWSPAAIKSALMTTAFPMTPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDY 586
           VKSFHP+WSPAAIKSALMT+AFPM+PK+NTDAE AYGAGHLNPVNAINPGLVYDAEE+DY
Sbjct: 491 VKSFHPSWSPAAIKSALMTSAFPMSPKLNTDAELAYGAGHLNPVNAINPGLVYDAEELDY 550

Query: 587 IKFLCGQGYSTENLRLVTGDQSNCSVVTKTAASDLNYPSFALL--SKSKRSFSRVYHRTV 646
           IKFLCGQGYST++LRLV+GD SNCS VTKTAASDLNYPSF L+  S S+R  SRVYHRTV
Sbjct: 551 IKFLCGQGYSTKDLRLVSGDHSNCSDVTKTAASDLNYPSFGLVINSTSQRLISRVYHRTV 610

Query: 647 TNVGLPRSTYKAVIQAPPGLKVTVRPATLSFSSLGQKISFTVRVKATAP-GGKVISGSLT 706
           TNVGLP STYKAVI+APPGLKVTVRPATLSF SLGQKISFTV V+A A   GKV+SGSLT
Sbjct: 611 TNVGLPVSTYKAVIKAPPGLKVTVRPATLSFRSLGQKISFTVTVRAKADVAGKVVSGSLT 670

Query: 707 WDDGVHLVRSPIVAFVIP 722
           WDDGVHLVRSPIV+FVIP
Sbjct: 671 WDDGVHLVRSPIVSFVIP 684

BLAST of Spg009742 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 664.8 bits (1714), Expect = 7.6e-191
Identity = 355/690 (51.45%), Postives = 462/690 (66.96%), Query Frame = 0

Query: 36  HTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEEARKLAEMEEVVSVFPSEKKQLHT 95
           H ++LQ+++ T  AS  LV SY RSF+GFAA L++ E++KL  M+EVVSVFPS+  +L T
Sbjct: 15  HLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTT 74

Query: 96  TRSWDFMGLFEEARRTSL-ESDLVVGMLDTGIWPESESFSDEGFGPPPAKWKGECQPSSN 155
           TRSWDF+G  E+ARR S+ ESD++VG++D+GIWPESESF DEGFGPPP KWKG C+    
Sbjct: 75  TRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLK 134

Query: 156 FTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTHTSSTAAGNLVSGASLLGLALGTSR 215
           F CNNK+IGARF+    F       S RD EGHGTHT+STAAGN V  AS  GLA GT+R
Sbjct: 135 FACNNKLIGARFYNK--FAD-----SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTAR 194

Query: 216 GGAPSARIAVYKICWSDGCFDADILAAFDNAIADGVDIISISVGGFIAKNYFNDSIAIGA 275
           GG PSARIA YK+C+ + C D DILAAFD+AIADGVD+ISIS+      N  N S+AIG+
Sbjct: 195 GGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGS 254

Query: 276 FHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVAASTIDRKFVTNVKLGNGETFQGISV 335
           FHAM  GI+T+ S GN+GP   S+ NVSPW ++VAAS  DR+F+  V LGNG+   GISV
Sbjct: 255 FHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISV 314

Query: 336 NTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFPGSLDVNQVQGKIVLCDEIGDGEGAL 395
           NTF+L     P++Y     N +   +++ + +C  G +D   V+GKIVLCD+      A 
Sbjct: 315 NTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAY 374

Query: 396 NSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKKVFEYLRSTSNPEAIIEKSTTIEDLL 455
            +GA+G I+Q+    D AF+ P PAS L     K +  Y+ S   P+A I ++  I D  
Sbjct: 375 LAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDRE 434

Query: 456 APSVVSFSSRGPNLVTLDILKPDLAAPGVDILASWSEASTATSL--PGDKRIVPFNIISG 515
           AP V SFSSRGP+ V  ++LKPD++APG++ILA++S  ++ +S   P DKR V ++++SG
Sbjct: 435 APYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSG 494

Query: 516 TSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFPMTPKVNTDAEFAYGAGHLNPVNA 575
           TSMACPH  GVAAYVKSFHP WSP+AIKSA+MTTA PM  K N + EFAYG+G +NP  A
Sbjct: 495 TSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKA 554

Query: 576 INPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQSNCSVVTKTAASDLNYPSFALLSKS 635
            +PGLVY+ E  DY+K LC +G+ +  L   +G    CS   +T   DLNYP+      S
Sbjct: 555 SDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS--ERTEVKDLNYPTMTTFVSS 614

Query: 636 KRSFSRVYHRTVTNVGLPRSTYKA-VIQAPPGLKVTVRPATLSFSSLGQKISFTVRVKA- 695
              F+  + RTVTNVG P STYKA V+   P L++++ P  L F  L +K SF V +   
Sbjct: 615 LDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGK 674

Query: 696 TAPGGKVISGSLTWDDGVHLVRSPIVAFVI 721
               G  +S S+ W DG H VRSPIVA+ I
Sbjct: 675 ELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of Spg009742 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 648.7 bits (1672), Expect = 5.7e-186
Identity = 352/715 (49.23%), Postives = 484/715 (67.69%), Query Frame = 0

Query: 14  RQVYVVYMGKSSEGGFTAASERHTNMLQQV-LTTSDASKSLVYSYHRSFSGFAARLNEEE 73
           +  Y++Y+G   +       + H N+L  + ++  +A +  VYSY ++F+ FAA+L+  E
Sbjct: 35  KDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKERKVYSYTKAFNAFAAKLSPHE 94

Query: 74  ARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEEARR-TSLESDLVVGMLDTGIWPESE 133
           A+K+ EMEEVVSV  ++ ++LHTT+SWDF+GL   A+R    E D+++G+LDTGI P+SE
Sbjct: 95  AKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSE 154

Query: 134 SFSDEGFGPPPAKWKGECQPSSNFT-CNNKIIGARFFRSEPFEGEGDILSPRDTEGHGTH 193
           SF D G GPPPAKWKG C P  NFT CNNKIIGA++F+ +     G++ SP D +GHGTH
Sbjct: 155 SFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTH 214

Query: 194 TSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWS-DGCFDADILAAFDNAIADG 253
           TSST AG LV+ ASL G+A GT+RG  PSAR+A+YK+CW+  GC D DILA F+ AI DG
Sbjct: 215 TSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDG 274

Query: 254 VDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVA 313
           V+IISIS+GG IA +Y +DSI++G+FHAM+ GILT  S GN GPS  ++TN  PW L+VA
Sbjct: 275 VEIISISIGGPIA-DYSSDSISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVA 334

Query: 314 ASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFP 373
           AS IDR F + + LGNG++F G+ ++ F    K  PL+   DA   T   ++ L+R+CF 
Sbjct: 335 ASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKNTD--DKYLARYCFS 394

Query: 374 GSLDVNQVQGKIVLCDEIGDG-EGALNS-GAVGTIMQDANDQDVAFLFPLPASKLDLNTG 433
            SLD  +V+GK+++C   G G E  + S G  G I+      D A +F  PA+ ++ + G
Sbjct: 395 DSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIFMAPATSVNSSVG 454

Query: 434 KKVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILA 493
             ++ Y+ ST +  A+I+K+  +  + AP V SFSSRGPN  ++ +LKPD+AAPG+DILA
Sbjct: 455 DIIYRYINSTRSASAVIQKTRQV-TIPAPFVASFSSRGPNPGSIRLLKPDIAAPGIDILA 514

Query: 494 SWSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTA 553
           +++   + T L GD +   F I+SGTSMACPH  GVAAYVKSFHP W+PAAIKSA++T+A
Sbjct: 515 AFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKSFHPDWTPAAIKSAIITSA 574

Query: 554 FPMTPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQ 613
            P++ +VN DAEFAYG G +NP  A +PGLVYD ++I Y++FLCG+GY+   L  + G +
Sbjct: 575 KPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYNATTLAPLVGTR 634

Query: 614 S-NCSVVTKTAASD-LNYPSFAL-LSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGL 673
           S +CS +      D LNYP+  L L  +K S   V+ R VTNVG P S Y A ++AP G+
Sbjct: 635 SVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTNVGPPSSVYTATVRAPKGV 694

Query: 674 KVTVRPATLSFSSLGQKISFTVRVKA-TAPGGKVISGSLTWDDGVHLVRSPIVAF 719
           ++TV P +LSFS   QK SF V VKA     GK++SG L W    H VRSPIV +
Sbjct: 695 EITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWKSPRHSVRSPIVIY 744

BLAST of Spg009742 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 645.6 bits (1664), Expect = 4.8e-185
Identity = 372/739 (50.34%), Postives = 493/739 (66.71%), Query Frame = 0

Query: 13  ERQVYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSL-VYSYHRSFSGFAARLNEE 72
           ER+ Y+VYMG+++E     A+E H N+L  V+     ++ L +YSY ++ +GF ARL   
Sbjct: 31  ERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESKARELKIYSYGKNINGFVARLFPH 90

Query: 73  EARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEE--ARRTSLESDLVVGMLDTGIWPE 132
           EA KL+  E VVSVF + ++QLHTTRSWDF+GL E    R   +ES+++VG+LDTGI  E
Sbjct: 91  EAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVGIESNIIVGVLDTGIDVE 150

Query: 133 SESFSDEGFGPPPAKWKGECQPSSNFT-CNNKIIGARFF--RSEPF-EGEGDILSPRDTE 192
           S SF+D+G GPPPAKWKG+C   +NFT CNNK+IGA++F  +SE   +GEGD  +  D +
Sbjct: 151 SPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEGD--TAADHD 210

Query: 193 GHGTHTSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNA 252
           GHGTHTSST AG  VS ASL G+A GT+RGG PSARIA YK+CW  GC D D+LAAFD A
Sbjct: 211 GHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDMLAAFDEA 270

Query: 253 IADGVDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWS 312
           I+DGVDIISIS+GG  +  +F D IAIGAFHAMK GILT+ S GN+GP L +++N++PW 
Sbjct: 271 ISDGVDIISISIGG-ASLPFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWV 330

Query: 313 LSVAASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSR 372
           ++VAA+++DRKF T VKLGNG T  GIS+N F+   K+ PL     A N +AG     S 
Sbjct: 331 MTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGSLASNLSAGGYGEPST 390

Query: 373 FCFPGSLDVNQVQGKIVLCDEIGDGEGALNSG-----------AVGTIMQDANDQDVAFL 432
            C PG+L  ++V GK+V C E G  EG  N G             G I+Q     D+A  
Sbjct: 391 -CEPGTLGEDKVMGKVVYC-EAGREEGG-NGGQGQDHVVRSLKGAGVIVQLLEPTDMATS 450

Query: 433 FPLPASKLDLNTGKKVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDIL 492
             +  S +    G K+ EY+ ST NP+A+I K+ T + +LAPS+ SFS+RGP  ++ +IL
Sbjct: 451 TLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTK-MLAPSISSFSARGPQRISPNIL 510

Query: 493 KPDLAAPGVDILASWSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTW 552
           KPD++APG++ILA++S+ ++ T  P D R   F+I+SGTSMACPHA   AAYVKSFHP W
Sbjct: 511 KPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACPHAAAAAAYVKSFHPDW 570

Query: 553 SPAAIKSALMTTAFPMTPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQG 612
           SPAAIKSALMTTA PM  K N +AE +YG+G +NP  AI+PGLVYD  E  Y++FLC +G
Sbjct: 571 SPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPGLVYDITEDAYLRFLCKEG 630

Query: 613 YSTENLRLVTGDQS--------NCSVVTKTAASD-LNYPSF-ALLSKSKRSFSRVYHRTV 672
           Y++ ++ L+TGD S        NC  + +   SD LNYPS    ++ ++   S V++RTV
Sbjct: 631 YNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQVNSTEAKVSEVFYRTV 690

Query: 673 TNVGLPRSTYKAVIQAPPGLKVTVRPATLSFSSLGQKISFTVRVKA----TAPGGKVISG 719
           TNVG   STY A + AP GL+V V P  +SF    +K +F V +      T  G  ++S 
Sbjct: 691 TNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVIDGVWDETMKG--IVSA 750

BLAST of Spg009742 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 639.8 bits (1649), Expect = 2.6e-183
Identity = 359/716 (50.14%), Postives = 470/716 (65.64%), Query Frame = 0

Query: 13  ERQVYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEE 72
           ++Q Y+VYMG             HT++LQ V   S     LV +Y RSF+GFAARL + E
Sbjct: 31  DKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSIEDRLVRNYKRSFNGFAARLTKSE 90

Query: 73  ARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFEE---ARRTSLESDLVVGMLDTGIWPE 132
              LA M+EVVSVFP++K +L TT SW+FMGL E     R T +ESD ++G++D+GI+PE
Sbjct: 91  REILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKRNTIIESDTIIGVIDSGIYPE 150

Query: 133 SESFSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGT 192
           S+SFS +GFGPPP KWKG C+   NFT NNK+IGAR++  +    EG   S RD  GHG+
Sbjct: 151 SDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYYTPKL---EGFPESARDYMGHGS 210

Query: 193 HTSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKIC--WSDGCFDADILAAFDNAIA 252
           HT+STAAGN V   S  GL  GT+RGG P+ARIAVYK+C    DGC    ILAAFD+AIA
Sbjct: 211 HTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGVDGCTTDGILAAFDDAIA 270

Query: 253 DGVDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLS 312
           D VDII+IS+GG  +  +  D IAIGAFHAM  GIL  NS GNSGP  +++ +++PW  +
Sbjct: 271 DKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGNSGPEPSTVASIAPWMFT 330

Query: 313 VAASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFC 372
           VAAS  +R FVT V LGNG+T  G SVN+FDL  K  PL+Y   A   ++    + + FC
Sbjct: 331 VAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGKSA---SSSCGAASAGFC 390

Query: 373 FPGSLDVNQVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTG 432
            PG LD  +V+GKIVLCD   + + A   GA+ +I++ ++  DVA +F  P S L  +  
Sbjct: 391 SPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVR-SHRTDVASIFSFPVSVLLEDDY 450

Query: 433 KKVFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILA 492
             V  Y+ ST NP+A + KS TI +  AP V S+ SRGPN +  DILKPD+ APG +I+A
Sbjct: 451 NTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDILKPDITAPGSEIVA 510

Query: 493 SWSEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTA 552
           ++S    A     D R V +++ +GTSM+CPH  GVAAY+KSFHP WSP+ I+SA+MTTA
Sbjct: 511 AYS--PDAPPSISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTA 570

Query: 553 FPM---TPKVNTDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVT 612
           +PM   T   N  AEFAYGAGH++P+ AI+PGLVY+A + D+I FLCG  Y+ +NLRL++
Sbjct: 571 WPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLIS 630

Query: 613 GDQSNCS-VVTKTAASDLNYPSFALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPG 672
           GD S+C+   TK+   +LNYPS      + + F  ++ RTVTNVG P +TYKA +     
Sbjct: 631 GDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKVVGSK- 690

Query: 673 LKVTVRPATLSFSSLGQKISFTVRVKATAPGGK-VISGSLTWDDGVHLVRSPIVAF 719
           LKV V PA LS  SL +K SFTV      P  + ++S  L W DGVH VRSPIV +
Sbjct: 691 LKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Spg009742 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 639.4 bits (1648), Expect = 3.4e-183
Identity = 344/717 (47.98%), Postives = 469/717 (65.41%), Query Frame = 0

Query: 13  ERQVYVVYMGKSSEGGFTAASERHTNMLQQVLTTSDASKSLVYSYHRSFSGFAARLNEEE 72
           + QVY+VYMG  S       +  H ++LQQV   S     LV SY RSF+GFAARL E E
Sbjct: 29  DTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESE 88

Query: 73  ARKLAEMEEVVSVFPSEKKQLHTTRSWDFMGLFE---EARRTSLESDLVVGMLDTGIWPE 132
              +AE+E VVSVFP++  QLHTT SWDFMG+ E     R  ++ESD ++G++DTGIWPE
Sbjct: 89  RTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPE 148

Query: 133 SESFSDEGFGPPPAKWKGECQPSSNFTCNNKIIGARFFRSEPFEGEGDILSPRDTEGHGT 192
           S+SFSD+GFGPPP KWKG C    NFTCNNK+IGAR + SE           RDT GHGT
Sbjct: 149 SKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSE---------GTRDTSGHGT 208

Query: 193 HTSSTAAGNLVSGASLLGLALGTSRGGAPSARIAVYKICWSDGCFDADILAAFDNAIADG 252
           HT+STAAGN V   S  G+  GT RGG P++RIA YK+C   GC    +L++FD+AIADG
Sbjct: 209 HTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADG 268

Query: 253 VDIISISVGGFIAKNYFNDSIAIGAFHAMKNGILTSNSGGNSGPSLASITNVSPWSLSVA 312
           VD+I+IS+G      + +D IAIGAFHAM  GILT +S GNSGP   ++++V+PW  +VA
Sbjct: 269 VDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVA 328

Query: 313 ASTIDRKFVTNVKLGNGETFQGISVNTFDLGDKLLPLIYAGDAPNKTAGFNESLSRFCFP 372
           AST +R F+T V LGNG+T  G SVN FD+  K  PL+Y   A +     +   +  C P
Sbjct: 329 ASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSAC--DAKTAALCAP 388

Query: 373 GSLDVNQVQGKIVLCDEIGDGEGALNSGAVGTIMQDANDQDVAFLFPLPASKLDLNTGKK 432
             L+ ++V+GKI++C      + A + GA+  I+  +   DVAF   LPAS L     K 
Sbjct: 389 ACLNKSRVKGKILVCGGPSGYKIAKSVGAI-AIIDKSPRPDVAFTHHLPASGLKAKDFKS 448

Query: 433 VFEYLRSTSNPEAIIEKSTTIEDLLAPSVVSFSSRGPNLVTLDILKPDLAAPGVDILASW 492
           +  Y+ S  +P+A + K+ TI +  +P + SFSSRGPN + +DILKPD+ APGV+ILA++
Sbjct: 449 LVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAF 508

Query: 493 SEASTATSLPGDKRIVPFNIISGTSMACPHATGVAAYVKSFHPTWSPAAIKSALMTTAFP 552
           S     +    D R V +++ SGTSMACPH  GVAAYVK+F+P WSP+ I+SA+MTTA+P
Sbjct: 509 SPNGEPSE--DDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWP 568

Query: 553 MTPKVN--TDAEFAYGAGHLNPVNAINPGLVYDAEEIDYIKFLCGQGYSTENLRLVTGDQ 612
           +  K       EFAYGAGH++P+ A+NPGLVY+ ++ D+I FLCG  Y+++ L++++GD 
Sbjct: 569 VKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDT 628

Query: 613 SNCSVVTKTAASDLNYPSF-ALLSKSKRSFSRVYHRTVTNVGLPRSTYKAVIQAPPGLKV 672
             CS   K    +LNYPS  A LS +  +FS  ++RT+TNVG P STYK+ + A  G K+
Sbjct: 629 VKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKL 688

Query: 673 TVR--PATLSFSSLGQKISFTVRVKATAPGGKV-ISGSLTWDDGVHLVRSPIVAFVI 721
           +++  P+ L F ++ +K SF+V V  +    +V  S +L W DG H VRSPIV +++
Sbjct: 689 SIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6600966.10.0e+0086.69hypothetical protein SDJN03_06199, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7031587.10.0e+0085.97hypothetical protein SDJN02_05628, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022990086.10.0e+0086.73cucumisin-like [Cucurbita maxima][more]
XP_022139189.10.0e+0083.99cucumisin-like [Momordica charantia][more]
XP_038891640.10.0e+0085.69cucumisin-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q395478.7e-21654.97Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9FIF81.8e-19251.13Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9LLL88.0e-18549.23Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2... [more]
Q9LZS66.7e-18450.34Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3... [more]
F4JA913.7e-18250.14Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... [more]
Match NameE-valueIdentityDescription
A0A6J1JLZ20.0e+0086.73cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111487088 PE=3 SV=1[more]
A0A6J1CC810.0e+0083.99cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010157 PE=3 SV=1[more]
A0A5A7UDF90.0e+0081.59Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00108... [more]
A0A6J1GXX00.0e+0085.93cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458206 PE=3 SV=1[more]
A0A1S3CMK10.0e+0082.01cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103502667 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59190.17.6e-19151.45subtilase family protein [more]
AT4G00230.15.7e-18649.23xylem serine peptidase 1 [more]
AT5G03620.14.8e-18550.34Subtilisin-like serine endopeptidase family protein [more]
AT3G46840.12.6e-18350.14Subtilase family protein [more]
AT5G59090.13.4e-18347.98subtilase 4.12 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 511..527
score: 55.49
coord: 114..133
score: 27.57
coord: 183..196
score: 43.68
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 16..94
e-value: 3.6E-15
score: 56.4
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 620..716
e-value: 4.0E-29
score: 100.7
NoneNo IPR availableGENE3D3.50.30.30coord: 314..451
e-value: 2.3E-186
score: 622.1
NoneNo IPR availableGENE3D2.60.40.2310coord: 590..719
e-value: 3.0E-43
score: 148.7
NoneNo IPR availablePANTHERPTHR10795:SF649PUTATIVE-RELATEDcoord: 11..719
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 98..573
score: 25.375551
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 320..444
e-value: 3.12877E-18
score: 79.3799
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 116..586
e-value: 2.3E-186
score: 622.1
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 93..577
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 115..565
e-value: 6.3E-44
score: 150.3
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 10..94
e-value: 8.4E-27
score: 95.4
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 11..719
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 512..522
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 91..547
e-value: 7.16854E-134
score: 395.428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009742.1Spg009742.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity