Homology
BLAST of Spg009732 vs. NCBI nr
Match:
XP_016900177.1 (PREDICTED: cucumisin-like isoform X1 [Cucumis melo] >KAA0034649.1 cucumisin-like isoform X1 [Cucumis melo var. makuwa])
HSP 1 Score: 835.9 bits (2158), Expect = 2.9e-238
Identity = 467/805 (58.01%), Postives = 523/805 (64.97%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 94 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTG 153
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YGPPPAKWKG CE SANFSCN I+G ARSYRSN DI GPRDS+GHG
Sbjct: 154 YGPPPAKWKGSCEVSANFSCN------NKIIG---ARSYRSNGEYPEGDIKGPRDSDGHG 213
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GGLVRQA+MLGLGLGTARGGVPSARIA+YKVCWSDGCSDADILAAFDDAIAD
Sbjct: 214 THTASIVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIAD 273
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G + YFN+SIAIGSFHAMKKGILTS + GN GP+ TI NFSPW+LSV
Sbjct: 274 GVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSV 333
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV +NTFDI G Q PLVYAGDIP APF+ SVSRLC E
Sbjct: 334 AASTTDRKFETKVELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFEN 393
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
T++L L KGKIVVCDS ++P G A A GIIMQDDS D+ S+P+P+SHLG + GA
Sbjct: 394 TIDLKLVKGKIVVCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTN-SFPIPASHLGPEAGA 453
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
ILSYINST NS+ TATI KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 454 LILSYINST-NSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILK----------- 513
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 514 ------------------------------------------------------------ 573
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 574 ------------------------------------------------------------ 633
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDLS PGVEILAAW I PP G E+D+++VLY+I+SGTSMACP
Sbjct: 634 -----------------PDLSGPGVEILAAWSPISPPSGAEEDSKRVLYNIISGTSMACP 693
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSP+ALKSAL+TT GHINPLGAV+PGLI
Sbjct: 694 HVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYGAGHINPLGAVHPGLI 738
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
Y+ASEIDY FLCGQ YTT LLQQVS DN CS NSDTVFDLNYPSF+L T SK INQ
Sbjct: 754 YDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQ 738
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+YRR VTNVGS + TYKATI+NP+K L I V PS+LSF+ LGEEQSFE++IKG +
Sbjct: 814 VYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLGEEQSFEVTIKGKIRK-NI 738
Query: 781 VSASLVWDDGEHKVTSPIVVFDDNV 786
SASLVWDDG+HKV SPI VFD N+
Sbjct: 874 ESASLVWDDGKHKVRSPITVFDANI 738
BLAST of Spg009732 vs. NCBI nr
Match:
XP_038891432.1 (cucumisin-like [Benincasa hispida])
HSP 1 Score: 833.2 bits (2151), Expect = 1.9e-237
Identity = 463/805 (57.52%), Postives = 523/805 (64.97%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 94 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTG 153
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YGPPP KWKG CE SANFSCN I+G R+YRS+ DI GPRDS+GHG
Sbjct: 154 YGPPPPKWKGSCEVSANFSCN------NKIIG---GRAYRSDGKHPEGDIKGPRDSDGHG 213
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GG+VRQASMLGLGLGTARGGVPSARIASYKVCWSDGC+DADILAAFDDAIAD
Sbjct: 214 THTASIVAGGVVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCTDADILAAFDDAIAD 273
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G + YFN+SIAIGSFHAMKKGILTS + GN GP+ +I NFSPW+LSV
Sbjct: 274 GVDIISGSLGGSGIRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTSIVNFSPWSLSV 333
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV INTFD+ G Q PLVYAGDIP APF+ SVSR+C E
Sbjct: 334 AASTTDRKFETKVELGDGREFNGVSINTFDLKGKQIPLVYAGDIPKAPFDSSVSRMCFEN 393
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
TV L L KGKIVVCDS ++P G A AAGIIMQDDSP+D+ S+P+P+SHL K GA
Sbjct: 394 TVELELVKGKIVVCDSLAVPGGVVAIKGAAGIIMQDDSPQDDTN-SFPIPASHLAPKPGA 453
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
ILSYINST NSL TATI+KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 454 LILSYINST-NSLPTATIMKSTERKRKRAPFVASFSSRGPNPITPNILK----------- 513
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 514 ------------------------------------------------------------ 573
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 574 ------------------------------------------------------------ 633
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDL+ PGVEILAAWPSI P G E+D+++V Y+I+SGTSMACP
Sbjct: 634 -----------------PDLTGPGVEILAAWPSIASPSGAEEDSKRVAYNILSGTSMACP 693
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSPAALKSAL+TT GHINPLGAV PGL+
Sbjct: 694 HVTAAAAYVKSFHPTWSPAALKSALITTAFSMSLKRNPDKEFGYGAGHINPLGAVQPGLV 738
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
YNASEIDY KFLCGQ Y T LLQ+VS DN CS NSDTVFDLNYPSF+L T SKSINQ
Sbjct: 754 YNASEIDYVKFLCGQGYNTELLQRVSEDNDTCSSNNSDTVFDLNYPSFALSTNISKSINQ 738
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+Y+R VTNVGS TYKATI+NP+K L ITV PS+LSF+ LGEEQ+F L+IKG S +
Sbjct: 814 VYKRIVTNVGSKYATYKATIINPWKNLEITVNPSVLSFKNLGEEQTFVLTIKGRISK-NI 738
Query: 781 VSASLVWDDGEHKVTSPIVVFDDNV 786
SASLVW+DG+HKV SPI +FD N+
Sbjct: 874 ESASLVWEDGKHKVRSPITIFDSNI 738
BLAST of Spg009732 vs. NCBI nr
Match:
TYK09200.1 (cucumisin-like [Cucumis melo var. makuwa])
HSP 1 Score: 828.6 bits (2139), Expect = 4.7e-236
Identity = 463/801 (57.80%), Postives = 519/801 (64.79%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 94 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTG 153
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YGPPPAKWKG CE SANFSCN I+G ARSYRSN DI GPRDS+GHG
Sbjct: 154 YGPPPAKWKGSCEVSANFSCN------NKIIG---ARSYRSNGEYPEGDIKGPRDSDGHG 213
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GGLVRQA+MLGLGLGTARGGVPSARIA+YKVCWSDGCSDADILAAFDDAIAD
Sbjct: 214 THTASIVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIAD 273
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G + YFN+SIAIGSFHAMKKGILTS + GN GP+ TI NFSPW+LSV
Sbjct: 274 GVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSV 333
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV +NTFDI G Q PLVYAGDIP APF+ SVSRLC E
Sbjct: 334 AASTTDRKFETKVELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFEN 393
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
T++L L KGKIVVCDS ++P G A A GIIMQDDS D+ S+P+P+SHLG + GA
Sbjct: 394 TIDLKLVKGKIVVCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTN-SFPIPASHLGPEAGA 453
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
ILSYINST NS+ TATI KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 454 LILSYINST-NSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILK----------- 513
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 514 ------------------------------------------------------------ 573
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 574 ------------------------------------------------------------ 633
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDLS PGVEILAAW I PP G E+D+++VLY+I+SGTSMACP
Sbjct: 634 -----------------PDLSGPGVEILAAWSPISPPSGAEEDSKRVLYNIISGTSMACP 693
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSP+ALKSAL+TT GHINPLGAV+PGLI
Sbjct: 694 HVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYGAGHINPLGAVHPGLI 734
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
Y+ASEIDY FLCGQ YTT LLQQVS DN CS NSDTVFDLNYPSF+L T SK INQ
Sbjct: 754 YDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQ 734
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+YRR VTNVGS + TYKATI+NP+K L I V PS+LSF+ LGEEQSFE++IKG +
Sbjct: 814 VYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLGEEQSFEVTIKGKIRK-NI 734
Query: 781 VSASLVWDDGEHKVTSPIVVF 782
SASLVWDDG+HKV SPI +
Sbjct: 874 ESASLVWDDGKHKVRSPITTY 734
BLAST of Spg009732 vs. NCBI nr
Match:
KAE8648818.1 (hypothetical protein Csa_008881 [Cucumis sativus])
HSP 1 Score: 824.7 bits (2129), Expect = 6.7e-235
Identity = 461/807 (57.13%), Postives = 521/807 (64.56%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 92 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAG 151
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YG PPAKWKG CE SANFSCN I+G ARSYRSN DI GPRDS+GHG
Sbjct: 152 YGSPPAKWKGSCEVSANFSCN------NKIIG---ARSYRSNGEYPEGDIKGPRDSDGHG 211
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GGLVR+ASMLGLGLGTARGGVPSARIA+YKVCWSDGCSDADILAAFDDAIAD
Sbjct: 212 THTASIVAGGLVRRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIAD 271
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G A+ YFN+SIAIGSFHAMKKGILTS + GN GP+ TI NFSPW+LSV
Sbjct: 272 GVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSV 331
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV +NTFDI G Q PLVYAGDIP APF+ SVSRLC E
Sbjct: 332 AASTTDRKFETKVELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFEN 391
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
TV+L L KGKIVVCDS ++P G A A GIIMQDDS D+ S+P+P+SHLG K GA
Sbjct: 392 TVDLKLVKGKIVVCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTN-SFPIPASHLGPKAGA 451
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
+LSYINST NS+ TATI KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 452 LVLSYINST-NSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILK----------- 511
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 512 ------------------------------------------------------------ 571
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 572 ------------------------------------------------------------ 631
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDLS PGVEILAAW + PP G E+D ++VLY+I+SGTSMACP
Sbjct: 632 -----------------PDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACP 691
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSP+ALKSAL+TT GHINPLGAV+PGLI
Sbjct: 692 HVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLI 738
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
Y+ASEIDY +FLCGQ YTT LLQ VS DN CS NSDTVFDLNYPSF+L T SK INQ
Sbjct: 752 YDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQ 738
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+Y+R VTNVGS TYKAT++NP+K L I V PS+LSF+ LGE+QSFE++I+G
Sbjct: 812 VYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLGEKQSFEVTIRGKIRK-DI 738
Query: 781 VSASLVWDDGEHKVTSPIVVFDDNVVN 788
SASLVWDDG+HKV SPI VF N+++
Sbjct: 872 ESASLVWDDGKHKVRSPITVFIANIIH 738
BLAST of Spg009732 vs. NCBI nr
Match:
XP_031741129.1 (cucumisin-like [Cucumis sativus])
HSP 1 Score: 824.7 bits (2129), Expect = 6.7e-235
Identity = 461/807 (57.13%), Postives = 521/807 (64.56%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 95 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAG 154
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YG PPAKWKG CE SANFSCN I+G ARSYRSN DI GPRDS+GHG
Sbjct: 155 YGSPPAKWKGSCEVSANFSCN------NKIIG---ARSYRSNGEYPEGDIKGPRDSDGHG 214
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GGLVR+ASMLGLGLGTARGGVPSARIA+YKVCWSDGCSDADILAAFDDAIAD
Sbjct: 215 THTASIVAGGLVRRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIAD 274
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G A+ YFN+SIAIGSFHAMKKGILTS + GN GP+ TI NFSPW+LSV
Sbjct: 275 GVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSV 334
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV +NTFDI G Q PLVYAGDIP APF+ SVSRLC E
Sbjct: 335 AASTTDRKFETKVELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFEN 394
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
TV+L L KGKIVVCDS ++P G A A GIIMQDDS D+ S+P+P+SHLG K GA
Sbjct: 395 TVDLKLVKGKIVVCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTN-SFPIPASHLGPKAGA 454
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
+LSYINST NS+ TATI KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 455 LVLSYINST-NSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILK----------- 514
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 515 ------------------------------------------------------------ 574
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 575 ------------------------------------------------------------ 634
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDLS PGVEILAAW + PP G E+D ++VLY+I+SGTSMACP
Sbjct: 635 -----------------PDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACP 694
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSP+ALKSAL+TT GHINPLGAV+PGLI
Sbjct: 695 HVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLI 741
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
Y+ASEIDY +FLCGQ YTT LLQ VS DN CS NSDTVFDLNYPSF+L T SK INQ
Sbjct: 755 YDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQ 741
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+Y+R VTNVGS TYKAT++NP+K L I V PS+LSF+ LGE+QSFE++I+G
Sbjct: 815 VYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLGEKQSFEVTIRGKIRK-DI 741
Query: 781 VSASLVWDDGEHKVTSPIVVFDDNVVN 788
SASLVWDDG+HKV SPI VF N+++
Sbjct: 875 ESASLVWDDGKHKVRSPITVFIANIIH 741
BLAST of Spg009732 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 601.3 bits (1549), Expect = 1.6e-170
Identity = 356/801 (44.44%), Postives = 446/801 (55.68%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGV+S+F NE +L TTRSWDF+GF V R VES+I+VGVLDTGIWPESPSF +EG
Sbjct: 95 MEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEG 154
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
+ PPP KWKG CE S NF CN R I+G ARSY + D+ GPRD+NGHG
Sbjct: 155 FSPPPPKWKGTCETSNNFRCN------RKIIG---ARSYHIGRPISPGDVNGPRDTNGHG 214
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AST GGLV QA++ GLGLGTARGGVP ARIA+YKVCW+DGCSD DILAA+DDAIAD
Sbjct: 215 THTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIAD 274
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G +HYF ++IAIGSFHA+++GILTS SAGN GPN T A+ SPW LSV
Sbjct: 275 GVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSV 334
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AAST DRKF++ +Q+G+G+ F+GV INTFD +PLV DIPN F+ S SR C ++
Sbjct: 335 AASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNTGFDKSTSRFCTDK 394
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGAA---AAGIIMQDDSPKDNLFWSYPLPSSHLGSKHG 360
+VN L KGKIVVC++ P F + AAG++M ++ + SYPLPSS L
Sbjct: 395 SVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNT--RDYADSYPLPSSVLDPNDL 454
Query: 361 AQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGS 420
L YI S ++ ATI KST N AP+V SFSSRGPN T ++K
Sbjct: 455 LATLRYIYSIRS--PGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIK---------- 514
Query: 421 VREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGN 480
Sbjct: 515 ------------------------------------------------------------ 574
Query: 481 LRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRA 540
Sbjct: 575 ------------------------------------------------------------ 634
Query: 541 RFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMAC 600
PD+S PGVEILAAWPS+ P G R L++I+SGTSM+C
Sbjct: 635 ------------------PDISGPGVEILAAWPSVAPVGGIR---RNTLFNIISGTSMSC 694
Query: 601 PHATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGL 660
PH T YVK+++P+WSPAA+KSALMTT GH+NPL AV PGL
Sbjct: 695 PHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGL 727
Query: 661 IYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSIN 720
+Y+A+E DY KFLCGQ Y T +++++GD C+ N+ V+DLNYPSF L S++ N
Sbjct: 755 VYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFN 727
Query: 721 QIYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSIS 780
Q + R +T+V +TY+A I P + L I+V P++LSF LG+ +SF L+++GS
Sbjct: 815 QYFNRTLTSVAPQASTYRAMISAP-QGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGF- 727
BLAST of Spg009732 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 490.7 bits (1262), Expect = 3.0e-137
Identity = 305/807 (37.79%), Postives = 420/807 (52.04%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSE--QVDRVPSVESDIIVGVLDTGIWPESPSFSN 60
ME V+S+FP+ KL+LQTT SW+FMG E + R S+ESD I+GV+D+GI+PES SFS+
Sbjct: 97 MERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSD 156
Query: 61 EGYGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNG 120
+G+GPPP KWKG C NF+CN ++G AR Y + S AN RD +G
Sbjct: 157 QGFGPPPKKWKGTCAGGKNFTCN------NKVIG---ARDYTAKSK--ANQTA--RDYSG 216
Query: 121 HGTHVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAI 180
HGTH AS G V ++ GLG GTARGGVP+ARIA YKVC ++GC +++AFDDAI
Sbjct: 217 HGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAI 276
Query: 181 ADGVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTL 240
ADGVD+IS SI + + IAIG+FHAM G+LT +AGN GP ++T+ + +PW
Sbjct: 277 ADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVF 336
Query: 241 SVAASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCI 300
SVAAS T+R F++ + LGDG+ G +NT+D+NGT +PLVY + + +RLC
Sbjct: 337 SVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCE 396
Query: 301 ERTVNLTLAKGKIVVCDS-RSIPIGFGAAAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHG 360
+ ++ L KGKIV+CDS + + A G I+++ P S+P+ S L +
Sbjct: 397 PKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFIRSFPV--SFLSNDDY 456
Query: 361 AQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGS 420
++SY+NSTKN AT+LKS E N+RAP+VASFSSRGP+ I ILK
Sbjct: 457 KSLVSYMNSTKN--PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILK---------- 516
Query: 421 VREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGN 480
Sbjct: 517 ------------------------------------------------------------ 576
Query: 481 LRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRA 540
Sbjct: 577 ------------------------------------------------------------ 636
Query: 541 RFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMAC 600
PD++APGVEILAA+ P E DTR+V YS++SGTSMAC
Sbjct: 637 ------------------PDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMAC 696
Query: 601 PHATAAVAYVKSFHPSWSPAALKSALMTT--------------------GHINPLGAVNP 660
PH AYVK+FHP WSP+ ++SA+MTT GH++P+ A+NP
Sbjct: 697 PHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINP 738
Query: 661 GLIYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTV-FDLNYPSFSLHTPTSK 720
GL+Y ++ D+ FLCG NYT+ L+ +SGDN C+ E S T+ +LNYP+ S +K
Sbjct: 757 GLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTK 738
Query: 721 SINQIYRRKVTNVGSTNTTYKATIMN-PFKTLNITVKPSILSFETLGEEQSFELSIKG-S 780
N ++R VTNVG +TY A ++ P L+I V P +LS +++ E+QSF +++ S
Sbjct: 817 PFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDS 738
Query: 781 TSSISFVSASLVWDDGEHKVTSPIVVF 782
+ VSA+L+W DG H V SPI+V+
Sbjct: 877 IGTKQPVSANLIWSDGTHNVRSPIIVY 738
BLAST of Spg009732 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 488.0 bits (1255), Expect = 2.0e-136
Identity = 300/808 (37.13%), Postives = 418/808 (51.73%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSE--QVDRVPSVESDIIVGVLDTGIWPESPSFSN 60
+EGV+S+FPN+ LQL TT SWDFMG E R ++ESD I+GV+DTGIWPES SFS+
Sbjct: 95 IEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSD 154
Query: 61 EGYGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNG 120
+G+GPPP KWKG C NF+CN ++G AR Y S G RD++G
Sbjct: 155 KGFGPPPKKWKGVCSGGKNFTCN------NKLIG---ARDYTSE---------GTRDTSG 214
Query: 121 HGTHVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAI 180
HGTH AST G V+ S G+G GT RGGVP++RIA+YKVC GCS +L++FDDAI
Sbjct: 215 HGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAI 274
Query: 181 ADGVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTL 240
ADGVD+I+ SIG + + ++ IAIG+FHAM KGILT +SAGN+GP T+++ +PW
Sbjct: 275 ADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIF 334
Query: 241 SVAASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCI 300
+VAASTT+R F++ + LG+G+ G +N FD+ G ++PLVY ++ + + LC
Sbjct: 335 TVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCA 394
Query: 301 ERTVNLTLAKGKIVVCDSRSIPIGFGAA--AAGIIMQDDSPKDNLFWSYPLPSSHLGSKH 360
+N + KGKI+VC P G+ A I + D SP+ ++ +++ LP+S L +K
Sbjct: 395 PACLNKSRVKGKILVCGG---PSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKD 454
Query: 361 GAQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVG 420
++SYI S + A +LK+ N +P++ASFSSRGPN I ILK
Sbjct: 455 FKSLVSYIESQDS--PQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILK--------- 514
Query: 421 SVREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPG 480
Sbjct: 515 ------------------------------------------------------------ 574
Query: 481 NLRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNR 540
Sbjct: 575 ------------------------------------------------------------ 634
Query: 541 ARFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMA 600
PD++APGVEILAA+ P EDDTR+V YS+ SGTSMA
Sbjct: 635 -------------------PDITAPGVEILAAFSPNGEP--SEDDTRRVKYSVFSGTSMA 694
Query: 601 CPHATAAVAYVKSFHPSWSPAALKSALMTT--------------------GHINPLGAVN 660
CPH AYVK+F+P WSP+ ++SA+MTT GH++P+ A+N
Sbjct: 695 CPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALN 729
Query: 661 PGLIYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLH-TPTS 720
PGL+Y + D+ FLCG NYT+ L+ +SGD + CS +N +LNYPS S + T
Sbjct: 755 PGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTD 729
Query: 721 KSINQIYRRKVTNVGSTNTTYKATIM-NPFKTLNITVKPSILSFETLGEEQSFELSIKGS 780
+ + + R +TNVG+ N+TYK+ ++ L+I V PS+L F+T+ E+QSF +++ GS
Sbjct: 815 STFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGS 729
Query: 781 -TSSISFVSASLVWDDGEHKVTSPIVVF 782
S SA+L+W DG H V SPIVV+
Sbjct: 875 DVDSEVPSSANLIWSDGTHNVRSPIVVY 729
BLAST of Spg009732 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 486.5 bits (1251), Expect = 5.7e-136
Identity = 306/803 (38.11%), Postives = 408/803 (50.81%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
M+ V+S+FP++ +L TTRSWDF+GF E+ R ESD+IVGV+D+GIWPES SF +EG
Sbjct: 94 MKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEG 153
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
+GPPP KWKG C+ F+CN ++G AR Y + RD GHG
Sbjct: 154 FGPPPKKWKGSCKGGLKFACN------NKLIG---ARFYNKFAD-------SARDEEGHG 213
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AST G V+ AS GL GTARGGVPSARIA+YKVC+ + C+D DILAAFDDAIAD
Sbjct: 214 THTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIAD 273
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVD+IS SI A + N S+AIGSFHAM +GI+T+ SAGN GP+ ++AN SPW ++V
Sbjct: 274 GVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITV 333
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AAS TDR+F+ + LG+G+ G+ +NTF++NGT+FP+VY ++ + + + + C
Sbjct: 334 AASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNV-SRNCSQAQAGYCSSG 393
Query: 301 TVNLTLAKGKIVVCDS-RSIPIGFGAAAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGAQ 360
V+ L KGKIV+CD + A A G+I+Q+ D+ F P P+S LG +
Sbjct: 394 CVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAF-VVPFPASSLGFEDYKS 453
Query: 361 ILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSVR 420
I SYI S + A IL++ E + AP V SFSSRGP+ + ++LK
Sbjct: 454 IKSYIESAEP--PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLK------------ 513
Query: 421 EKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNLR 480
Sbjct: 514 ------------------------------------------------------------ 573
Query: 481 GRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRARF 540
Sbjct: 574 ------------------------------------------------------------ 633
Query: 541 PDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPP--FGREDDTRQVLYSIMSGTSMAC 600
PD+SAPG+EILAA+ + P F +D R V YS+MSGTSMAC
Sbjct: 634 ----------------PDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMAC 693
Query: 601 PHATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGL 660
PH AYVKSFHP WSP+A+KSA+MTT G INP A +PGL
Sbjct: 694 PHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGL 725
Query: 661 IYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSIN 720
+Y DY K LC + + + L SG N+ CS V DLNYP+ + + N
Sbjct: 754 VYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE--VKDLNYPTMTTFVSSLDPFN 725
Query: 721 QIYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKG-STSSI 780
++R VTNVG N+TYKA+++ L I+++P IL F L E++SF ++I G
Sbjct: 814 VTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDG 725
Query: 781 SFVSASLVWDDGEHKVTSPIVVF 782
SFVS+S+VW DG H V SPIV +
Sbjct: 874 SFVSSSVVWSDGSHSVRSPIVAY 725
BLAST of Spg009732 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 475.7 bits (1223), Expect = 1.0e-132
Identity = 294/807 (36.43%), Postives = 416/807 (51.55%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSE--QVDRVPSVESDIIVGVLDTGIWPESPSFSN 60
M GV+S+FPN+KLQLQTT SWDFMG E + R P+VESD I+GV+D+GI PES SFS+
Sbjct: 94 MVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSD 153
Query: 61 EGYGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNG 120
+G+GPPP KWKG C NF+CN ++G AR Y S G RD +G
Sbjct: 154 KGFGPPPQKWKGVCSGGKNFTCN------NKLIG---ARDYTSE---------GTRDMDG 213
Query: 121 HGTHVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAI 180
HGTH AST G V AS G+G GT RGGVP++R+A+YKVC GCS +L+AFDDAI
Sbjct: 214 HGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAI 273
Query: 181 ADGVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTL 240
ADGVD+I+ SIG K A + N+ IAIG+FHAM KG+LT SAGN+GP +++ +PW L
Sbjct: 274 ADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWIL 333
Query: 241 SVAASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCI 300
+VAASTT+R F++ + LG+G+ G +N +++ G +PLVY ++ + + LC
Sbjct: 334 TVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESAGLCE 393
Query: 301 ERTVNLTLAKGKIVVCDS-RSIPIGFGAAAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHG 360
V+ + KGKI+VC + I A G+I + +PK ++ + +PLP++ L ++
Sbjct: 394 LSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYR--TPKPDVAFIHPLPAAGLLTEDF 453
Query: 361 AQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGS 420
++SY+ ST + A +LK+ N +P++ASFSSRGPN I ILK
Sbjct: 454 ESLVSYLESTDS--PQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILK---------- 513
Query: 421 VREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGN 480
Sbjct: 514 ------------------------------------------------------------ 573
Query: 481 LRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRA 540
Sbjct: 574 ------------------------------------------------------------ 633
Query: 541 RFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMAC 600
PD++APGVEILAA+ P +DDTR V YS++SGTSM+C
Sbjct: 634 ------------------PDITAPGVEILAAYSPAGEP--SQDDTRHVKYSVLSGTSMSC 693
Query: 601 PHATAAVAYVKSFHPSWSPAALKSALMTT--------------------GHINPLGAVNP 660
PH AYVK+F+P WSP+ ++SA+MTT GH++P+ A NP
Sbjct: 694 PHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNP 728
Query: 661 GLIYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLH-TPTSK 720
GL+Y + D+ FLCG NYT+ +L+ +SG+ + CS +LNYPS S + +
Sbjct: 754 GLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGT 728
Query: 721 SINQIYRRKVTNVGSTNTTYKATIM-NPFKTLNITVKPSILSFETLGEEQSFELSIKGST 780
+ + R +TNVG+ N+TY + ++ L++ + PS+LSF+T+ E+QSF +++ GS
Sbjct: 814 TFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSN 728
Query: 781 -SSISFVSASLVWDDGEHKVTSPIVVF 782
S SA+L+W DG H V SPIVV+
Sbjct: 874 LDSEVPSSANLIWSDGTHNVRSPIVVY 728
BLAST of Spg009732 vs. ExPASy TrEMBL
Match:
A0A5A7SZX9 (Cucumisin-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G006680 PE=3 SV=1)
HSP 1 Score: 835.9 bits (2158), Expect = 1.4e-238
Identity = 467/805 (58.01%), Postives = 523/805 (64.97%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 94 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTG 153
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YGPPPAKWKG CE SANFSCN I+G ARSYRSN DI GPRDS+GHG
Sbjct: 154 YGPPPAKWKGSCEVSANFSCN------NKIIG---ARSYRSNGEYPEGDIKGPRDSDGHG 213
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GGLVRQA+MLGLGLGTARGGVPSARIA+YKVCWSDGCSDADILAAFDDAIAD
Sbjct: 214 THTASIVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIAD 273
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G + YFN+SIAIGSFHAMKKGILTS + GN GP+ TI NFSPW+LSV
Sbjct: 274 GVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSV 333
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV +NTFDI G Q PLVYAGDIP APF+ SVSRLC E
Sbjct: 334 AASTTDRKFETKVELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFEN 393
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
T++L L KGKIVVCDS ++P G A A GIIMQDDS D+ S+P+P+SHLG + GA
Sbjct: 394 TIDLKLVKGKIVVCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTN-SFPIPASHLGPEAGA 453
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
ILSYINST NS+ TATI KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 454 LILSYINST-NSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILK----------- 513
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 514 ------------------------------------------------------------ 573
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 574 ------------------------------------------------------------ 633
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDLS PGVEILAAW I PP G E+D+++VLY+I+SGTSMACP
Sbjct: 634 -----------------PDLSGPGVEILAAWSPISPPSGAEEDSKRVLYNIISGTSMACP 693
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSP+ALKSAL+TT GHINPLGAV+PGLI
Sbjct: 694 HVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYGAGHINPLGAVHPGLI 738
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
Y+ASEIDY FLCGQ YTT LLQQVS DN CS NSDTVFDLNYPSF+L T SK INQ
Sbjct: 754 YDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQ 738
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+YRR VTNVGS + TYKATI+NP+K L I V PS+LSF+ LGEEQSFE++IKG +
Sbjct: 814 VYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLGEEQSFEVTIKGKIRK-NI 738
Query: 781 VSASLVWDDGEHKVTSPIVVFDDNV 786
SASLVWDDG+HKV SPI VFD N+
Sbjct: 874 ESASLVWDDGKHKVRSPITVFDANI 738
BLAST of Spg009732 vs. ExPASy TrEMBL
Match:
A0A1S4DW16 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489381 PE=3 SV=1)
HSP 1 Score: 835.9 bits (2158), Expect = 1.4e-238
Identity = 467/805 (58.01%), Postives = 523/805 (64.97%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 94 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTG 153
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YGPPPAKWKG CE SANFSCN I+G ARSYRSN DI GPRDS+GHG
Sbjct: 154 YGPPPAKWKGSCEVSANFSCN------NKIIG---ARSYRSNGEYPEGDIKGPRDSDGHG 213
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GGLVRQA+MLGLGLGTARGGVPSARIA+YKVCWSDGCSDADILAAFDDAIAD
Sbjct: 214 THTASIVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIAD 273
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G + YFN+SIAIGSFHAMKKGILTS + GN GP+ TI NFSPW+LSV
Sbjct: 274 GVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSV 333
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV +NTFDI G Q PLVYAGDIP APF+ SVSRLC E
Sbjct: 334 AASTTDRKFETKVELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFEN 393
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
T++L L KGKIVVCDS ++P G A A GIIMQDDS D+ S+P+P+SHLG + GA
Sbjct: 394 TIDLKLVKGKIVVCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTN-SFPIPASHLGPEAGA 453
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
ILSYINST NS+ TATI KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 454 LILSYINST-NSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILK----------- 513
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 514 ------------------------------------------------------------ 573
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 574 ------------------------------------------------------------ 633
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDLS PGVEILAAW I PP G E+D+++VLY+I+SGTSMACP
Sbjct: 634 -----------------PDLSGPGVEILAAWSPISPPSGAEEDSKRVLYNIISGTSMACP 693
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSP+ALKSAL+TT GHINPLGAV+PGLI
Sbjct: 694 HVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYGAGHINPLGAVHPGLI 738
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
Y+ASEIDY FLCGQ YTT LLQQVS DN CS NSDTVFDLNYPSF+L T SK INQ
Sbjct: 754 YDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQ 738
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+YRR VTNVGS + TYKATI+NP+K L I V PS+LSF+ LGEEQSFE++IKG +
Sbjct: 814 VYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLGEEQSFEVTIKGKIRK-NI 738
Query: 781 VSASLVWDDGEHKVTSPIVVFDDNV 786
SASLVWDDG+HKV SPI VFD N+
Sbjct: 874 ESASLVWDDGKHKVRSPITVFDANI 738
BLAST of Spg009732 vs. ExPASy TrEMBL
Match:
A0A5D3CCZ5 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001930 PE=3 SV=1)
HSP 1 Score: 828.6 bits (2139), Expect = 2.3e-236
Identity = 463/801 (57.80%), Postives = 519/801 (64.79%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 94 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTG 153
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YGPPPAKWKG CE SANFSCN I+G ARSYRSN DI GPRDS+GHG
Sbjct: 154 YGPPPAKWKGSCEVSANFSCN------NKIIG---ARSYRSNGEYPEGDIKGPRDSDGHG 213
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GGLVRQA+MLGLGLGTARGGVPSARIA+YKVCWSDGCSDADILAAFDDAIAD
Sbjct: 214 THTASIVAGGLVRQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIAD 273
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G + YFN+SIAIGSFHAMKKGILTS + GN GP+ TI NFSPW+LSV
Sbjct: 274 GVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSV 333
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV +NTFDI G Q PLVYAGDIP APF+ SVSRLC E
Sbjct: 334 AASTTDRKFETKVELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFEN 393
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
T++L L KGKIVVCDS ++P G A A GIIMQDDS D+ S+P+P+SHLG + GA
Sbjct: 394 TIDLKLVKGKIVVCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTN-SFPIPASHLGPEAGA 453
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
ILSYINST NS+ TATI KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 454 LILSYINST-NSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILK----------- 513
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 514 ------------------------------------------------------------ 573
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 574 ------------------------------------------------------------ 633
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDLS PGVEILAAW I PP G E+D+++VLY+I+SGTSMACP
Sbjct: 634 -----------------PDLSGPGVEILAAWSPISPPSGAEEDSKRVLYNIISGTSMACP 693
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSP+ALKSAL+TT GHINPLGAV+PGLI
Sbjct: 694 HVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYGAGHINPLGAVHPGLI 734
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
Y+ASEIDY FLCGQ YTT LLQQVS DN CS NSDTVFDLNYPSF+L T SK INQ
Sbjct: 754 YDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQ 734
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+YRR VTNVGS + TYKATI+NP+K L I V PS+LSF+ LGEEQSFE++IKG +
Sbjct: 814 VYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLGEEQSFEVTIKGKIRK-NI 734
Query: 781 VSASLVWDDGEHKVTSPIVVF 782
SASLVWDDG+HKV SPI +
Sbjct: 874 ESASLVWDDGKHKVRSPITTY 734
BLAST of Spg009732 vs. ExPASy TrEMBL
Match:
A0A0A0KWL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G613590 PE=3 SV=1)
HSP 1 Score: 824.7 bits (2129), Expect = 3.3e-235
Identity = 461/807 (57.13%), Postives = 521/807 (64.56%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
MEGVIS+FPN K QL TTRSWDFMGFSEQV RVP+VES++IVGVLD+GIWPESPSF + G
Sbjct: 95 MEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAG 154
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YG PPAKWKG CE SANFSCN I+G ARSYRSN DI GPRDS+GHG
Sbjct: 155 YGSPPAKWKGSCEVSANFSCN------NKIIG---ARSYRSNGEYPEGDIKGPRDSDGHG 214
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AS V GGLVR+ASMLGLGLGTARGGVPSARIA+YKVCWSDGCSDADILAAFDDAIAD
Sbjct: 215 THTASIVAGGLVRRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIAD 274
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G A+ YFN+SIAIGSFHAMKKGILTS + GN GP+ TI NFSPW+LSV
Sbjct: 275 GVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSV 334
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AASTTDRKF + ++LGDGR F GV +NTFDI G Q PLVYAGDIP APF+ SVSRLC E
Sbjct: 335 AASTTDRKFETKVELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFEN 394
Query: 301 TVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGA 360
TV+L L KGKIVVCDS ++P G A A GIIMQDDS D+ S+P+P+SHLG K GA
Sbjct: 395 TVDLKLVKGKIVVCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTN-SFPIPASHLGPKAGA 454
Query: 361 QILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSV 420
+LSYINST NS+ TATI KSTE K +RAP VASFSSRGPNPITP+ILK
Sbjct: 455 LVLSYINST-NSIPTATIKKSTERKRKRAPSVASFSSRGPNPITPNILK----------- 514
Query: 421 REKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNL 480
Sbjct: 515 ------------------------------------------------------------ 574
Query: 481 RGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRAR 540
Sbjct: 575 ------------------------------------------------------------ 634
Query: 541 FPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMACP 600
PDLS PGVEILAAW + PP G E+D ++VLY+I+SGTSMACP
Sbjct: 635 -----------------PDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACP 694
Query: 601 HATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGLI 660
H TAA AYVKSFHP+WSP+ALKSAL+TT GHINPLGAV+PGLI
Sbjct: 695 HVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLI 741
Query: 661 YNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSINQ 720
Y+ASEIDY +FLCGQ YTT LLQ VS DN CS NSDTVFDLNYPSF+L T SK INQ
Sbjct: 755 YDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQ 741
Query: 721 IYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSISF 780
+Y+R VTNVGS TYKAT++NP+K L I V PS+LSF+ LGE+QSFE++I+G
Sbjct: 815 VYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLGEKQSFEVTIRGKIRK-DI 741
Query: 781 VSASLVWDDGEHKVTSPIVVFDDNVVN 788
SASLVWDDG+HKV SPI VF N+++
Sbjct: 875 ESASLVWDDGKHKVRSPITVFIANIIH 741
BLAST of Spg009732 vs. ExPASy TrEMBL
Match:
A0A6J1E2G4 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1)
HSP 1 Score: 812.8 bits (2098), Expect = 1.3e-231
Identity = 461/806 (57.20%), Postives = 517/806 (64.14%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
M+GVIS+FPNEK QL TTRSWDFMG S+QV RVPSVESDIIVGVLDTGIWPESPSF +EG
Sbjct: 92 MKGVISVFPNEKKQLHTTRSWDFMGLSQQVGRVPSVESDIIVGVLDTGIWPESPSFLDEG 151
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
YGPPP +WKG CEAS NFSCN I+G ARSYR+N NDI GPRDSNGHG
Sbjct: 152 YGPPPPRWKGSCEASLNFSCN------NKIIG---ARSYRTNGQYPINDIQGPRDSNGHG 211
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH ASTV GGLVRQASMLGLG GTARGGVPSARIASYK+CWSD CSDADILAAFDDAIAD
Sbjct: 212 THTASTVAGGLVRQASMLGLGSGTARGGVPSARIASYKICWSDDCSDADILAAFDDAIAD 271
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVDIIS S+G + YFN+S+AIG+FHAMKKGILTS SAGN GP TI NFSPW+LSV
Sbjct: 272 GVDIISFSVGGHTPKDYFNDSMAIGAFHAMKKGILTSMSAGNDGPQSFTIRNFSPWSLSV 331
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPN--APFNGSVSRLCI 300
AASTT+R +LS IQLGDGR F GV INTFD+NGTQ+PLVYAG+IPN FNGS+SR C+
Sbjct: 332 AASTTNRSYLSGIQLGDGRSFNGVTINTFDLNGTQYPLVYAGNIPNITGGFNGSISRFCL 391
Query: 301 ERTVNLTLAKGKIVVCDSRSIPIGFGA--AAAGIIMQDDSPKDNLFWSYPLPSSHLGSKH 360
+V+ KGKIV+CD P G+ A GIIMQD +PKD L + +PLP+SHLG++
Sbjct: 392 PNSVDRESVKGKIVLCDFFVSPKNLGSLEGAIGIIMQDTNPKD-LTFPFPLPASHLGTQE 451
Query: 361 GAQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVG 420
GA I SY N T L TATILKSTEGK + P VASFSSRGPNPITP ILK
Sbjct: 452 GALISSYANLT--GLPTATILKSTEGKYKATPFVASFSSRGPNPITPDILK--------- 511
Query: 421 SVREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPG 480
Sbjct: 512 ------------------------------------------------------------ 571
Query: 481 NLRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNR 540
Sbjct: 572 ------------------------------------------------------------ 631
Query: 541 ARFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMA 600
PDLS PGVEILAAW I PP G E+DTRQ+L++I+SGTSM+
Sbjct: 632 -------------------PDLSGPGVEILAAWSPIGPPSGAEEDTRQLLFNIISGTSMS 691
Query: 601 CPHATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPG 660
CPHATA AYVK+FHPSWSPAALKSALMTT GHINPL AVNPG
Sbjct: 692 CPHATAVAAYVKTFHPSWSPAALKSALMTTAFPMRADLNPDAEFAYGSGHINPLSAVNPG 735
Query: 661 LIYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSI 720
LIYNA+EIDY +FLCGQ Y+T L+QQVSGDN CS + D VFDLNYPSF+L T S SI
Sbjct: 752 LIYNATEIDYVRFLCGQGYSTKLVQQVSGDNSSCSRGDIDLVFDLNYPSFALSTSISTSI 735
Query: 721 NQIYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKGSTSSI 780
+Q+YRR+VTNVGS N+TYKA + P LNITV PS+LSF+ LGEE SFEL+I+GS SS
Sbjct: 812 SQVYRRRVTNVGSANSTYKAIVSGP-SGLNITVNPSVLSFKALGEELSFELTIEGSISS- 735
Query: 781 SFVSASLVWDDGEHKVTSPIVVFDDN 785
S SASLVWDDG+HKV SPIVVFD+N
Sbjct: 872 SIASASLVWDDGKHKVKSPIVVFDEN 735
BLAST of Spg009732 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 490.7 bits (1262), Expect = 2.1e-138
Identity = 305/807 (37.79%), Postives = 420/807 (52.04%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSE--QVDRVPSVESDIIVGVLDTGIWPESPSFSN 60
ME V+S+FP+ KL+LQTT SW+FMG E + R S+ESD I+GV+D+GI+PES SFS+
Sbjct: 97 MERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSD 156
Query: 61 EGYGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNG 120
+G+GPPP KWKG C NF+CN ++G AR Y + S AN RD +G
Sbjct: 157 QGFGPPPKKWKGTCAGGKNFTCN------NKVIG---ARDYTAKSK--ANQTA--RDYSG 216
Query: 121 HGTHVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAI 180
HGTH AS G V ++ GLG GTARGGVP+ARIA YKVC ++GC +++AFDDAI
Sbjct: 217 HGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAI 276
Query: 181 ADGVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTL 240
ADGVD+IS SI + + IAIG+FHAM G+LT +AGN GP ++T+ + +PW
Sbjct: 277 ADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVF 336
Query: 241 SVAASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCI 300
SVAAS T+R F++ + LGDG+ G +NT+D+NGT +PLVY + + +RLC
Sbjct: 337 SVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCE 396
Query: 301 ERTVNLTLAKGKIVVCDS-RSIPIGFGAAAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHG 360
+ ++ L KGKIV+CDS + + A G I+++ P S+P+ S L +
Sbjct: 397 PKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFIRSFPV--SFLSNDDY 456
Query: 361 AQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGS 420
++SY+NSTKN AT+LKS E N+RAP+VASFSSRGP+ I ILK
Sbjct: 457 KSLVSYMNSTKN--PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILK---------- 516
Query: 421 VREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGN 480
Sbjct: 517 ------------------------------------------------------------ 576
Query: 481 LRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRA 540
Sbjct: 577 ------------------------------------------------------------ 636
Query: 541 RFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMAC 600
PD++APGVEILAA+ P E DTR+V YS++SGTSMAC
Sbjct: 637 ------------------PDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMAC 696
Query: 601 PHATAAVAYVKSFHPSWSPAALKSALMTT--------------------GHINPLGAVNP 660
PH AYVK+FHP WSP+ ++SA+MTT GH++P+ A+NP
Sbjct: 697 PHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINP 738
Query: 661 GLIYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTV-FDLNYPSFSLHTPTSK 720
GL+Y ++ D+ FLCG NYT+ L+ +SGDN C+ E S T+ +LNYP+ S +K
Sbjct: 757 GLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTK 738
Query: 721 SINQIYRRKVTNVGSTNTTYKATIMN-PFKTLNITVKPSILSFETLGEEQSFELSIKG-S 780
N ++R VTNVG +TY A ++ P L+I V P +LS +++ E+QSF +++ S
Sbjct: 817 PFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDS 738
Query: 781 TSSISFVSASLVWDDGEHKVTSPIVVF 782
+ VSA+L+W DG H V SPI+V+
Sbjct: 877 IGTKQPVSANLIWSDGTHNVRSPIIVY 738
BLAST of Spg009732 vs. TAIR 10
Match:
AT5G59090.2 (subtilase 4.12 )
HSP 1 Score: 490.0 bits (1260), Expect = 3.7e-138
Identity = 300/803 (37.36%), Postives = 418/803 (52.05%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSE--QVDRVPSVESDIIVGVLDTGIWPESPSFSN 60
+EGV+S+FPN+ LQL TT SWDFMG E R ++ESD I+GV+DTGIWPES SFS+
Sbjct: 95 IEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSD 154
Query: 61 EGYGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNG 120
+G+GPPP KWKG C NF+CN ++G AR Y S G RD++G
Sbjct: 155 KGFGPPPKKWKGVCSGGKNFTCN------NKLIG---ARDYTSE---------GTRDTSG 214
Query: 121 HGTHVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAI 180
HGTH AST G V+ S G+G GT RGGVP++RIA+YKVC GCS +L++FDDAI
Sbjct: 215 HGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAI 274
Query: 181 ADGVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTL 240
ADGVD+I+ SIG + + ++ IAIG+FHAM KGILT +SAGN+GP T+++ +PW
Sbjct: 275 ADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIF 334
Query: 241 SVAASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCI 300
+VAASTT+R F++ + LG+G+ G +N FD+ G ++PLVY ++ + + LC
Sbjct: 335 TVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCA 394
Query: 301 ERTVNLTLAKGKIVVCDSRSIPIGFGAA--AAGIIMQDDSPKDNLFWSYPLPSSHLGSKH 360
+N + KGKI+VC P G+ A I + D SP+ ++ +++ LP+S L +K
Sbjct: 395 PACLNKSRVKGKILVCGG---PSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKD 454
Query: 361 GAQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVG 420
++SYI S + A +LK+ N +P++ASFSSRGPN I ILK
Sbjct: 455 FKSLVSYIESQDS--PQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILK--------- 514
Query: 421 SVREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPG 480
Sbjct: 515 ------------------------------------------------------------ 574
Query: 481 NLRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNR 540
Sbjct: 575 ------------------------------------------------------------ 634
Query: 541 ARFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMA 600
PD++APGVEILAA+ P EDDTR+V YS+ SGTSMA
Sbjct: 635 -------------------PDITAPGVEILAAFSPNGEP--SEDDTRRVKYSVFSGTSMA 694
Query: 601 CPHATAAVAYVKSFHPSWSPAALKSALMTT---------------GHINPLGAVNPGLIY 660
CPH AYVK+F+P WSP+ ++SA+MTT GH++P+ A+NPGL+Y
Sbjct: 695 CPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAKGRGIASTEFAYGAGHVDPMAALNPGLVY 724
Query: 661 NASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLH-TPTSKSINQ 720
+ D+ FLCG NYT+ L+ +SGD + CS +N +LNYPS S + T + +
Sbjct: 755 ELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSV 724
Query: 721 IYRRKVTNVGSTNTTYKATIM-NPFKTLNITVKPSILSFETLGEEQSFELSIKGS-TSSI 780
+ R +TNVG+ N+TYK+ ++ L+I V PS+L F+T+ E+QSF +++ GS S
Sbjct: 815 TFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSE 724
Query: 781 SFVSASLVWDDGEHKVTSPIVVF 782
SA+L+W DG H V SPIVV+
Sbjct: 875 VPSSANLIWSDGTHNVRSPIVVY 724
BLAST of Spg009732 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 488.0 bits (1255), Expect = 1.4e-137
Identity = 300/808 (37.13%), Postives = 418/808 (51.73%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSE--QVDRVPSVESDIIVGVLDTGIWPESPSFSN 60
+EGV+S+FPN+ LQL TT SWDFMG E R ++ESD I+GV+DTGIWPES SFS+
Sbjct: 95 IEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSD 154
Query: 61 EGYGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNG 120
+G+GPPP KWKG C NF+CN ++G AR Y S G RD++G
Sbjct: 155 KGFGPPPKKWKGVCSGGKNFTCN------NKLIG---ARDYTSE---------GTRDTSG 214
Query: 121 HGTHVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAI 180
HGTH AST G V+ S G+G GT RGGVP++RIA+YKVC GCS +L++FDDAI
Sbjct: 215 HGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAI 274
Query: 181 ADGVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTL 240
ADGVD+I+ SIG + + ++ IAIG+FHAM KGILT +SAGN+GP T+++ +PW
Sbjct: 275 ADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIF 334
Query: 241 SVAASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCI 300
+VAASTT+R F++ + LG+G+ G +N FD+ G ++PLVY ++ + + LC
Sbjct: 335 TVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCA 394
Query: 301 ERTVNLTLAKGKIVVCDSRSIPIGFGAA--AAGIIMQDDSPKDNLFWSYPLPSSHLGSKH 360
+N + KGKI+VC P G+ A I + D SP+ ++ +++ LP+S L +K
Sbjct: 395 PACLNKSRVKGKILVCGG---PSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKD 454
Query: 361 GAQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVG 420
++SYI S + A +LK+ N +P++ASFSSRGPN I ILK
Sbjct: 455 FKSLVSYIESQDS--PQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILK--------- 514
Query: 421 SVREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPG 480
Sbjct: 515 ------------------------------------------------------------ 574
Query: 481 NLRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNR 540
Sbjct: 575 ------------------------------------------------------------ 634
Query: 541 ARFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMA 600
PD++APGVEILAA+ P EDDTR+V YS+ SGTSMA
Sbjct: 635 -------------------PDITAPGVEILAAFSPNGEP--SEDDTRRVKYSVFSGTSMA 694
Query: 601 CPHATAAVAYVKSFHPSWSPAALKSALMTT--------------------GHINPLGAVN 660
CPH AYVK+F+P WSP+ ++SA+MTT GH++P+ A+N
Sbjct: 695 CPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALN 729
Query: 661 PGLIYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLH-TPTS 720
PGL+Y + D+ FLCG NYT+ L+ +SGD + CS +N +LNYPS S + T
Sbjct: 755 PGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTD 729
Query: 721 KSINQIYRRKVTNVGSTNTTYKATIM-NPFKTLNITVKPSILSFETLGEEQSFELSIKGS 780
+ + + R +TNVG+ N+TYK+ ++ L+I V PS+L F+T+ E+QSF +++ GS
Sbjct: 815 STFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGS 729
Query: 781 -TSSISFVSASLVWDDGEHKVTSPIVVF 782
S SA+L+W DG H V SPIVV+
Sbjct: 875 DVDSEVPSSANLIWSDGTHNVRSPIVVY 729
BLAST of Spg009732 vs. TAIR 10
Match:
AT5G59090.3 (subtilase 4.12 )
HSP 1 Score: 487.6 bits (1254), Expect = 1.8e-137
Identity = 300/807 (37.17%), Postives = 417/807 (51.67%), Query Frame = 0
Query: 2 EGVISIFPNEKLQLQTTRSWDFMGFSE--QVDRVPSVESDIIVGVLDTGIWPESPSFSNE 61
EGV+S+FPN+ LQL TT SWDFMG E R ++ESD I+GV+DTGIWPES SFS++
Sbjct: 94 EGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDK 153
Query: 62 GYGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGH 121
G+GPPP KWKG C NF+CN ++G AR Y S G RD++GH
Sbjct: 154 GFGPPPKKWKGVCSGGKNFTCN------NKLIG---ARDYTSE---------GTRDTSGH 213
Query: 122 GTHVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIA 181
GTH AST G V+ S G+G GT RGGVP++RIA+YKVC GCS +L++FDDAIA
Sbjct: 214 GTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIA 273
Query: 182 DGVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLS 241
DGVD+I+ SIG + + ++ IAIG+FHAM KGILT +SAGN+GP T+++ +PW +
Sbjct: 274 DGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFT 333
Query: 242 VAASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIE 301
VAASTT+R F++ + LG+G+ G +N FD+ G ++PLVY ++ + + LC
Sbjct: 334 VAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAP 393
Query: 302 RTVNLTLAKGKIVVCDSRSIPIGFGAA--AAGIIMQDDSPKDNLFWSYPLPSSHLGSKHG 361
+N + KGKI+VC P G+ A I + D SP+ ++ +++ LP+S L +K
Sbjct: 394 ACLNKSRVKGKILVCGG---PSGYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDF 453
Query: 362 AQILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGS 421
++SYI S + A +LK+ N +P++ASFSSRGPN I ILK
Sbjct: 454 KSLVSYIESQDS--PQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILK---------- 513
Query: 422 VREKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGN 481
Sbjct: 514 ------------------------------------------------------------ 573
Query: 482 LRGRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRA 541
Sbjct: 574 ------------------------------------------------------------ 633
Query: 542 RFPDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPPFGREDDTRQVLYSIMSGTSMAC 601
PD++APGVEILAA+ P EDDTR+V YS+ SGTSMAC
Sbjct: 634 ------------------PDITAPGVEILAAFSPNGEP--SEDDTRRVKYSVFSGTSMAC 693
Query: 602 PHATAAVAYVKSFHPSWSPAALKSALMTT--------------------GHINPLGAVNP 661
PH AYVK+F+P WSP+ ++SA+MTT GH++P+ A+NP
Sbjct: 694 PHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNP 727
Query: 662 GLIYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLH-TPTSK 721
GL+Y + D+ FLCG NYT+ L+ +SGD + CS +N +LNYPS S + T
Sbjct: 754 GLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDS 727
Query: 722 SINQIYRRKVTNVGSTNTTYKATIM-NPFKTLNITVKPSILSFETLGEEQSFELSIKGS- 781
+ + + R +TNVG+ N+TYK+ ++ L+I V PS+L F+T+ E+QSF +++ GS
Sbjct: 814 TFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSD 727
BLAST of Spg009732 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 486.5 bits (1251), Expect = 4.1e-137
Identity = 306/803 (38.11%), Postives = 408/803 (50.81%), Query Frame = 0
Query: 1 MEGVISIFPNEKLQLQTTRSWDFMGFSEQVDRVPSVESDIIVGVLDTGIWPESPSFSNEG 60
M+ V+S+FP++ +L TTRSWDF+GF E+ R ESD+IVGV+D+GIWPES SF +EG
Sbjct: 58 MKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEG 117
Query: 61 YGPPPAKWKGRCEASANFSCNKYKQNLRTILGWHTARSYRSNSTMLANDIVGPRDSNGHG 120
+GPPP KWKG C+ F+CN ++G AR Y + RD GHG
Sbjct: 118 FGPPPKKWKGSCKGGLKFACN------NKLIG---ARFYNKFAD-------SARDEEGHG 177
Query: 121 THVASTVVGGLVRQASMLGLGLGTARGGVPSARIASYKVCWSDGCSDADILAAFDDAIAD 180
TH AST G V+ AS GL GTARGGVPSARIA+YKVC+ + C+D DILAAFDDAIAD
Sbjct: 178 THTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIAD 237
Query: 181 GVDIISCSIGAKRAQHYFNNSIAIGSFHAMKKGILTSASAGNTGPNLATIANFSPWTLSV 240
GVD+IS SI A + N S+AIGSFHAM +GI+T+ SAGN GP+ ++AN SPW ++V
Sbjct: 238 GVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITV 297
Query: 241 AASTTDRKFLSTIQLGDGRGFEGVGINTFDINGTQFPLVYAGDIPNAPFNGSVSRLCIER 300
AAS TDR+F+ + LG+G+ G+ +NTF++NGT+FP+VY ++ + + + + C
Sbjct: 298 AASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNV-SRNCSQAQAGYCSSG 357
Query: 301 TVNLTLAKGKIVVCDS-RSIPIGFGAAAAGIIMQDDSPKDNLFWSYPLPSSHLGSKHGAQ 360
V+ L KGKIV+CD + A A G+I+Q+ D+ F P P+S LG +
Sbjct: 358 CVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAF-VVPFPASSLGFEDYKS 417
Query: 361 ILSYINSTKNSLATATILKSTEGKNERAPIVASFSSRGPNPITPSILKTFAEVPAVGSVR 420
I SYI S + A IL++ E + AP V SFSSRGP+ + ++LK
Sbjct: 418 IKSYIESAEP--PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLK------------ 477
Query: 421 EKKREENKVRRYCLRALPTDLSRGSSLRARELLTGGRELACRVVGEEGRKGGDDGPGNLR 480
Sbjct: 478 ------------------------------------------------------------ 537
Query: 481 GRERARDLGLLTGKEKKSRGRERAIWVSHEQILAASRPRCLPLARRKRKLSGSLTNRARF 540
Sbjct: 538 ------------------------------------------------------------ 597
Query: 541 PDLDLGLLVRKEKNPVPDLSAPGVEILAAWPSIVPP--FGREDDTRQVLYSIMSGTSMAC 600
PD+SAPG+EILAA+ + P F +D R V YS+MSGTSMAC
Sbjct: 598 ----------------PDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMAC 657
Query: 601 PHATAAVAYVKSFHPSWSPAALKSALMTT------------------GHINPLGAVNPGL 660
PH AYVKSFHP WSP+A+KSA+MTT G INP A +PGL
Sbjct: 658 PHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGL 689
Query: 661 IYNASEIDYAKFLCGQNYTTMLLQQVSGDNILCSPENSDTVFDLNYPSFSLHTPTSKSIN 720
+Y DY K LC + + + L SG N+ CS V DLNYP+ + + N
Sbjct: 718 VYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE--VKDLNYPTMTTFVSSLDPFN 689
Query: 721 QIYRRKVTNVGSTNTTYKATIMNPFKTLNITVKPSILSFETLGEEQSFELSIKG-STSSI 780
++R VTNVG N+TYKA+++ L I+++P IL F L E++SF ++I G
Sbjct: 778 VTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDG 689
Query: 781 SFVSASLVWDDGEHKVTSPIVVF 782
SFVS+S+VW DG H V SPIV +
Sbjct: 838 SFVSSSVVWSDGSHSVRSPIVAY 689
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 1.6e-170 | 44.44 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FGU3 | 3.0e-137 | 37.79 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
| Q8L7D2 | 2.0e-136 | 37.13 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
| Q9FIF8 | 5.7e-136 | 38.11 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9FIG2 | 1.0e-132 | 36.43 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5A7SZX9 | 1.4e-238 | 58.01 | Cucumisin-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... | [more] |
| A0A1S4DW16 | 1.4e-238 | 58.01 | cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489381 PE=3 SV=1 | [more] |
| A0A5D3CCZ5 | 2.3e-236 | 57.80 | Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00193... | [more] |
| A0A0A0KWL9 | 3.3e-235 | 57.13 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G613590 PE=3 SV=1 | [more] |
| A0A6J1E2G4 | 1.3e-231 | 57.20 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430231 PE=3 SV=1 | [more] |
