Spg009731 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009731
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description4-coumarate--CoA ligase
Locationscaffold7: 5582729 .. 5588553 (-)
RNA-Seq ExpressionSpg009731
SyntenySpg009731
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAGCTTTATGAGTCGCGACGAAAAAAAAAAGAGAGAAACATCGATTATGTCGCTTTAAAGCCAAACAGAGCCTTATAGAACTTTTAATCCCACGAATTGTCAAAAAAAAACTTTTATTCCCACGAAAATTACATTGATTAGGATCACAAATCCATCGTCCCGCTCGATTCTTGATTCATCAGCGCAAAATCATGTCCGGCGAAGAATCCTGCACTGCTCCGGCAGCCGTGGAATACCGGCGGCGGAATTCTCCCGGCGGTTACAATGTGAATACTGCGGTTTATCATTCACTCATCCAGATTGATGAAGCTTCTAAGATCTCCACCAGGCCAGACCTCGACACGGCCACTTTCGTCTTATCGCAGTTTCCGACGGCTGAATCGCGAGTTGCTCTAATCGACTCAGTCACGAGCTCCCGAGTTACTTATGGACAACTCAGCGCTTCGATTCGTTCCCTTGCTTGTGGATTGTATCACGCACTCGGAGTTCGAAAAGGCGATGTGGTGTTTGTTTTATCGGCGAATTCTGTTCTGTATCCAGTGATCTGTTTGGCTGTGTTGTCGATTGGTGCGGTGATAACGACGGCCAATCCAGTGAATACTGCGTCGGAAATTGGAAAGCAAGTGCGTGATTCAGGCGCGAAACTCGCTGTATCGGCGCCTGAGGAGTTGCATAAAGTGAGTCCGACTGGAATTCCGACGATTCTAACTACTCGGCCTCCGTACGGGGATGTTTTATCGGTGGAAGAGTTGATCGAATCCTGTATCGAATCGTTTGAGCCACTGCCGAAAGCGGAAATGAGTCAGTCGGATACGGCGGCGATATTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAGTTCTAACGCATTCAAATCTCATCTCTGTTATGGAACTTCTGATCTGGTACGTGGACGCGAGTTCCTCTCAAAACGACGTGTTCTTGTGCTTCATTCCGATGTTTCACATCTACGGCCTCGTCTTCTTCGGGCTCGGACTCTTCTGCAGAGGAATCACCACTGTTTTGATGCAGAGATTCAATTTCCAGTCCATGATCGACGCAATTGAAAAATACAAAGTCAATAACATACCGGCGGTACCTCCGGTGATTTTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTATCGTCGCTGAGACGTATCGGATCGGGAGCTGCACCGCTGGGAAAAGACGTAGAAGAAGCTTTCCGAGAGAAATTTCCATGGGTGGAGCTCCGGCCAGGATATGGCTTAACGGAGAGTACTGGAGCAGCGACTATCACGATCACAGACAAGGACGCTAAAGCTCATCCAGGTACAGTTATTTATTTATGATCATCTTTCATATTCTTAGCACTCTTTTCATTTCTGAATCTGAAAATCAGTGGGTTAAAAAACACTTTTGCGTAATGGTTTGATCCTAAATTATTTATCTATCAATAAGAATTTAGTTTAAGAAGTTTCAAATTTATAATAATTTAGTTCTATCAAAATATTGATTTGATTTAGTGAAGTTTCTTTTCACTCTAGGAAGAACCAAATATGTTTATAAAGGATTTTGCTGACATTTTTTTAATGTAATTCAATTGTAACATTTTTTTGAAGTGTAGAACATCGTTAGTTTGGGGTTGTTGTAGCTTTTAAAATAATTGTTGTCAAATGTGTTTTTAAGAAAATCAACTACATAAGAAGTAAATTAGTGTTTAGTAAATTTCATATTTAAAGTGGGAAATCAAGTTACATTGTTTGTTAAATTAATACTAAATTGTTGCAAAATAAGTATAATGACCAAAATAGACATATTTCAATGGTCATTATACTTATTTCAATTAGGGGCAGTTGCATAGATGGCAAATTGGACCAAATTAATAGCAAATATTACAGTTGCAAACTAATTGCAAATATAGCAAAATTAATTGTACAAGACCAAAGAGTTGTTGTCAAATTCTATCAGTGTCTATCATTGATAGACTCTGATTATTCAAAGTATATCAGTGTCTATCGCTGATAGACTTCGATTAATCAAAATCTATCAGTGGCTATCACTGATAGATTTTGATTAATCAGAGCCTATTAGTGATAGTCACCGATAGATTTTGATTAATCAGAGTTTATGAGTGATAGACACCAGAGTCTATCGCTGATAAACTCTGATAATCGAAGTCTATCAGATAGACTCTGATAGATTTTGCTATATTTGCAATTTTTTTTACATGGTGCTATATTTGCTATTAATTTGGTCCAATTTGCCACCCACTACAACTTTCCTTTCAATTAAGCATGAGATTGTAATATTTGCCTTCAAAACTTTCCTAAGATAATTAAATAAATTTATATCATTTATGTTATAAATGATATTTTGTTTACTTTAAATAAATTGATTAAAATATTCATAGAAACAATTGTTATACAAAAAAAAAAAAAAAATTAAATTAAAGTGTATCAAACAAAATCTAATTTCAGTTTAAATATCAAAAAGTAAAGAAAAATAAATTAGTTGCAATAAATTAAATTTAAAAATAAAAAGAATAGGATGAGATAAGATAAATTGTGAATAATAATATTTGAAAATCTTTATTGTAGATAAAATATGGAAGAATAAAAGACGAATTTTAAATATTTGTTAGTTACACCAGACTTTTATAAAAGCAAAATCCCAAAGCACTCTTAACCGCTTTTGCAAACTAATGTGATTCAAAAAATGAGTTTCTTTAAATTATTAAATAATCATTTTAACCCAACACAAAAAACGGTTAAAATTTTTTTACAATCACTTTTTACAACTTCAAAATCAATTTCAAACGGGGCCTAAAAGTGCTTCACCAAATTAGTATTAGATCAAGTGGTATTAAATGAAAGAGAAATTCACATGGTAGAACTCCCTCTTCCTCTTTTGGAGGTTGAGTTACCTTTAAATCTACTGTTCATTTCAAAATTATGATACATTTAACCATTGATTCACATTCCTAAAAAGGTTCATGTGGAATGTTGATGTCGAGATTTTCTGCAAAGATCGTAGACATTGAAACAGGGGAGGGACTGCCGCCGATGAAAGAAGGCGAGTTGTGGCTCAAGAGTCCAACAATAATGAAAGAATATCTCGGAAATAAGGAAGCAACAGAAGCCACCATGGATAAAGAAGGATGGTTGAAAACAGGGGATCTTGGCTACATAGATGAAGATGGATTCGTTTACATTGTTGATCGAATCAAGGAACTGATCAAACACAATGGATACCAGGTATAAAACAAACACCTCAATTTGAACCATATAAATTATCACATCACAATCCGATTAAGCCCAAGAACTATGCAATATCAATGAAGGTTGCTCCAGCAGAACTTGAAACGATTCTTTTGAGCCATCCAGAAATTATCGATGCTGCCGTTATCCCGTGAGTGTTTTTTTTTCCCTCTCTCAATCAAGAACAAAAATTATTATGTAGCTGCCATTTCTACACGATTAGATGTATACGAGAAAAAATACAAAATATTTTCAATTTGTATCCAATAGGTACAAAAAAATCATATAGATTTTCGAATTCACATCCAAAACCAGATTGTGACGGTCTCATGCAATAATTTATCCTATTTAACATTGTACATTGCGATTGTTCTGTGTTGCAGAATGGTGGATGAAGCCGCTGGACAGATTCCTGTGGCTTGTGTGGTGAAAGCATCCAATTGTCAGCTTACAGAAGAACAAGTAATTCAATTTGTCGCTTCTCAGGTACTGTCTCCTTTGCACCGTAATTTTTAATTAATTTATTATATTTATGATTTGGAGAGTTTTCATAGTCGACCTAAATGGGCCCAATCTCCATCTTAATGGGCCTGGCCTAGCCCACGCATCCGTCCCACCGTGACGACTAATCAGCATAATGTGAATTAAATAATATAGAAATAATATCTTCAAAAGTAATTACAAGATTTAAAAGTAGACGTCAATCAATTGAAAATTTAGCAATTATTTGATGTTTTGTATATACATATATCTTTTATTACTATATGAATTATAAGTTAATTGTCAGAAATTATTAATAAATAAATAATTAACAATTAAATTTCAAAATTAGAATAGGGTGGAAGAGATTTAAATTTGATAGAATTTTCTAATTTCATTTAATAGGGTTGCTATAAACACTTATTTTACAAAAGTTTATTTAATTCACATGCTAGTTTATATAGTTTGAAGTTGTGAAGTTGATTTTTTTTTTTTTTTTTTTTTGAGTTTGTTTATTTCATATAATGGTAATCTAAGTTATATAAATTTAGGCTTTCAAAACTATGACTCAAATATATACTTTTACCTATCACAAATCTACTTTTTTTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTACTAATTAGAAATAAACTTAGATTTTTTGTCAAAAATAAATAAATAAATAAACTTAGATTTTTACAACTTATTTTTATGCACGATCTCAATCTAAACTATATACAAACTCAAACTTTTTAGTTCTAAATAATAGAAATGGAAGCTTATCTCTCAACAAAGAAATTTGAAATTTCTGTACATTTTATGTTTATCAGACCATGTTTTCTATCTATAGTTTCTAATGAAAATTTAGGTCTTGTTTAGCTATTAGCAATGGTATAAGCTTTACTTTTATTCATTTTCCAAAACAGGCCAAATAATTTAAGCCCTAATAAAGGTTTTTTTTTTTAATAATAAAGAAAAGTCCTTGATTTTCTGAAAGTAATTATAATTATCTCATAAACACTTTTTGGCATTTAATGATATTTGGTTAATTACCACCAAAAATCTAAAAGGAGATATGTTCAAATTTTCTCTAAATAAAAACAATAATTTTCCGTCTAAATTTAAAAGAATCTAATGATTTTGCAAAAGCCGAAAAAAGGAGTAATAGGCCCAATGATCTCTAGGCTCTTGAATTTACGTTCTTGTCTTTACTATTAAACTCCGAATTATTACTATTATCATGATTATGGATCTAATTTTAAATTAAAAAGATTGCATTAGATTATGAGGATTGCAATTAATACTCTTGTAAAAATAATTCCATCAATTTTTTTTTTTTTTTTTTTTTTTTGCAATATGGGGGTGGGGCATTCGAACCACAGACCTCGTGGTTATCAGTACATCTTTATGTCAGTTGAGCTATGCTTGTTGGCATAATTCCATCAATATTATATGATACAATAATTAATATTCATATATATTTTTTAAAAAAAAAAAGTGGCATGTAATAAAAGGATTGATTTTATTTTATGAGAAAATAAATTTACAAAAAAAAAAAAAAAAAATTGCAGGTGGCACCTTACAAGAAGGTAAGAGGAGTGAGATTTATGGATGCCATTCCAAGGTCCCTTGCAGGCAAAATCTTGAGGAAGGATCTTGTCTCACAATTCAAACAACAACAGCTTTTGTCCAAACTCTAAACAGCCTTAGAATAAATTAAATTATTGAGACATATCTATATGATATATATGATGGGAAAATAATTTTGAACCATATTTCTCTCCCTCAAGGAGAGAAAAATGCATTCATAACTATCATATAATAATTAATAACAAAAATTCACCAGAAGATCTCCAAGTGTGTATGGTTGTAAATTATTGATCTCATTTTCACTCAAAAATGTAAATTTTAAGTGATTGGGAGTAATTTTTTTTTTCAAGGTGTACGGGACAGGTGATGTATATATTTTTTTTACAACATGTGAGGTGAGGGATTTGAACCTTTACCTCTTAGGGGCTGTTTGGGGCACTGAATTGATTATAATATCTGGTGGTTATAATAATCTG

mRNA sequence

ATGTCCGGCGAAGAATCCTGCACTGCTCCGGCAGCCGTGGAATACCGGCGGCGGAATTCTCCCGGCGGTTACAATGTGAATACTGCGGTTTATCATTCACTCATCCAGATTGATGAAGCTTCTAAGATCTCCACCAGGCCAGACCTCGACACGGCCACTTTCGTCTTATCGCAGTTTCCGACGGCTGAATCGCGAGTTGCTCTAATCGACTCAGTCACGAGCTCCCGAGTTACTTATGGACAACTCAGCGCTTCGATTCGTTCCCTTGCTTGTGGATTGTATCACGCACTCGGAGTTCGAAAAGGCGATGTGGTGTTTGTTTTATCGGCGAATTCTGTTCTGTATCCAGTGATCTGTTTGGCTGTGTTGTCGATTGGTGCGGTGATAACGACGGCCAATCCAGTGAATACTGCGTCGGAAATTGGAAAGCAAGTGCGTGATTCAGGCGCGAAACTCGCTGTATCGGCGCCTGAGGAGTTGCATAAAGTGAGTCCGACTGGAATTCCGACGATTCTAACTACTCGGCCTCCGTACGGGGATGTTTTATCGGTGGAAGAGTTGATCGAATCCTGTATCGAATCGTTTGAGCCACTGCCGAAAGCGGAAATGAGTCAGTCGGATACGGCGGCGATATTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAGTTCTAACGCATTCAAATCTCATCTCTGTTATGGAACTTCTGATCTGGTACGTGGACGCGAGTTCCTCTCAAAACGACGTGTTCTTGTGCTTCATTCCGATGTTTCACATCTACGGCCTCGTCTTCTTCGGGCTCGGACTCTTCTGCAGAGGAATCACCACTGTTTTGATGCAGAGATTCAATTTCCAGTCCATGATCGACGCAATTGAAAAATACAAAGTCAATAACATACCGGCGGTACCTCCGGTGATTTTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTATCGTCGCTGAGACGTATCGGATCGGGAGCTGCACCGCTGGGAAAAGACGTAGAAGAAGCTTTCCGAGAGAAATTTCCATGGGTGGAGCTCCGGCCAGGATATGGCTTAACGGAGAGTACTGGAGCAGCGACTATCACGATCACAGACAAGGACGCTAAAGCTCATCCAGGTTCATGTGGAATGTTGATGTCGAGATTTTCTGCAAAGATCGTAGACATTGAAACAGGGGAGGGACTGCCGCCGATGAAAGAAGGCGAGTTGTGGCTCAAGAGTCCAACAATAATGAAAGAATATCTCGGAAATAAGGAAGCAACAGAAGCCACCATGGATAAAGAAGGATGGTTGAAAACAGGGGATCTTGGCTACATAGATGAAGATGGATTCGTTTACATTGTTGATCGAATCAAGGAACTGATCAAACACAATGGATACCAGGTTGCTCCAGCAGAACTTGAAACGATTCTTTTGAGCCATCCAGAAATTATCGATGCTGCCGTTATCCCAATGGTGGATGAAGCCGCTGGACAGATTCCTGTGGCTTGTGTGGTGAAAGCATCCAATTGTCAGCTTACAGAAGAACAAGTAATTCAATTTGTCGCTTCTCAGGTGGCACCTTACAAGAAGGTAAGAGGAGTGAGATTTATGGATGCCATTCCAAGGTCCCTTGCAGGCAAAATCTTGAGGAAGGATCTTGTCTCACAATTCAAACAACAACAGCTTTTGTCCAAACTCTAA

Coding sequence (CDS)

ATGTCCGGCGAAGAATCCTGCACTGCTCCGGCAGCCGTGGAATACCGGCGGCGGAATTCTCCCGGCGGTTACAATGTGAATACTGCGGTTTATCATTCACTCATCCAGATTGATGAAGCTTCTAAGATCTCCACCAGGCCAGACCTCGACACGGCCACTTTCGTCTTATCGCAGTTTCCGACGGCTGAATCGCGAGTTGCTCTAATCGACTCAGTCACGAGCTCCCGAGTTACTTATGGACAACTCAGCGCTTCGATTCGTTCCCTTGCTTGTGGATTGTATCACGCACTCGGAGTTCGAAAAGGCGATGTGGTGTTTGTTTTATCGGCGAATTCTGTTCTGTATCCAGTGATCTGTTTGGCTGTGTTGTCGATTGGTGCGGTGATAACGACGGCCAATCCAGTGAATACTGCGTCGGAAATTGGAAAGCAAGTGCGTGATTCAGGCGCGAAACTCGCTGTATCGGCGCCTGAGGAGTTGCATAAAGTGAGTCCGACTGGAATTCCGACGATTCTAACTACTCGGCCTCCGTACGGGGATGTTTTATCGGTGGAAGAGTTGATCGAATCCTGTATCGAATCGTTTGAGCCACTGCCGAAAGCGGAAATGAGTCAGTCGGATACGGCGGCGATATTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAGTTCTAACGCATTCAAATCTCATCTCTGTTATGGAACTTCTGATCTGGTACGTGGACGCGAGTTCCTCTCAAAACGACGTGTTCTTGTGCTTCATTCCGATGTTTCACATCTACGGCCTCGTCTTCTTCGGGCTCGGACTCTTCTGCAGAGGAATCACCACTGTTTTGATGCAGAGATTCAATTTCCAGTCCATGATCGACGCAATTGAAAAATACAAAGTCAATAACATACCGGCGGTACCTCCGGTGATTTTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTATCGTCGCTGAGACGTATCGGATCGGGAGCTGCACCGCTGGGAAAAGACGTAGAAGAAGCTTTCCGAGAGAAATTTCCATGGGTGGAGCTCCGGCCAGGATATGGCTTAACGGAGAGTACTGGAGCAGCGACTATCACGATCACAGACAAGGACGCTAAAGCTCATCCAGGTTCATGTGGAATGTTGATGTCGAGATTTTCTGCAAAGATCGTAGACATTGAAACAGGGGAGGGACTGCCGCCGATGAAAGAAGGCGAGTTGTGGCTCAAGAGTCCAACAATAATGAAAGAATATCTCGGAAATAAGGAAGCAACAGAAGCCACCATGGATAAAGAAGGATGGTTGAAAACAGGGGATCTTGGCTACATAGATGAAGATGGATTCGTTTACATTGTTGATCGAATCAAGGAACTGATCAAACACAATGGATACCAGGTTGCTCCAGCAGAACTTGAAACGATTCTTTTGAGCCATCCAGAAATTATCGATGCTGCCGTTATCCCAATGGTGGATGAAGCCGCTGGACAGATTCCTGTGGCTTGTGTGGTGAAAGCATCCAATTGTCAGCTTACAGAAGAACAAGTAATTCAATTTGTCGCTTCTCAGGTGGCACCTTACAAGAAGGTAAGAGGAGTGAGATTTATGGATGCCATTCCAAGGTCCCTTGCAGGCAAAATCTTGAGGAAGGATCTTGTCTCACAATTCAAACAACAACAGCTTTTGTCCAAACTCTAA

Protein sequence

MSGEESCTAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGDVLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLVSQFKQQQLLSKL
Homology
[Close]
BLAST of Spg009731 vs. NCBI nr
Match: XP_022994494.1 (4-coumarate--CoA ligase-like 5 [Cucurbita maxima])

HSP 1 Score: 990.7 bits (2560), Expect = 5.1e-285
Identity = 509/564 (90.25%), Postives = 534/564 (94.68%), Query Frame = 0 

Query: 1   MSGEESCTAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFP 60
           MS EESCTAPAAVE+R  NSPGGY+VNTAVYHS +Q+DEA KIST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPMNSPGGYDVNTAVYHSFVQLDEAPKISTSPDLDTATFVLSQFP 60

Query: 61  TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
           TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
           AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTGIPTILTT+P YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTQPSYGD 180

Query: 181 VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIW 240
           VLSVEELIESCIESFE +P+ +++QSD AAILYSSGTTGTSKGVVLTHSNLISV+ELL W
Sbjct: 181 VLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
           YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLG 420
           GAAT  ITDKDA+AHPGSCGMLM RF AKIVDIETGEGLPPMKEGE+WLKSPTIMKEYL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
           NKEATEATMDKEGWLKTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 IIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
           I+DAAVIP  DEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
           SLAGKILRKDLVSQFKQQQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQFKQQQVLSKL 564

[Close]
BLAST of Spg009731 vs. NCBI nr
Match: KAG7031989.1 (4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 988.4 bits (2554), Expect = 2.5e-284
Identity = 510/564 (90.43%), Postives = 535/564 (94.86%), Query Frame = 0 

Query: 1   MSGEESCTAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFP 60
           MS EESCTAPAAVE+R  NSPGGY+VNTAVYHSL+Q+DEASKIST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPMNSPGGYDVNTAVYHSLVQLDEASKISTSPDLDTATFVLSQFP 60

Query: 61  TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
           TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
           AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTGIPTILTTRP YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTRPSYGD 180

Query: 181 VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIW 240
           VLSVEELIESCIE+FE +PK +++QSDTAAILYSSGTTGTSKGVVLTHSNLISV+ELL W
Sbjct: 181 VLSVEELIESCIETFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
           YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLG 420
           GAAT  ITDKDA+AHPGSCGMLM RF AKIVDIETGEGLPPMKEGE+WLKSPTIMKEYL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
           NKEATEATMDKEGW+KTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 IIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
           I+DAAVIP  DEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
           SLAGKILRKDLVSQ K QQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQLK-QQVLSKL 563

[Close]
BLAST of Spg009731 vs. NCBI nr
Match: XP_022957594.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 985.7 bits (2547), Expect = 1.6e-283
Identity = 509/564 (90.25%), Postives = 533/564 (94.50%), Query Frame = 0 

Query: 1   MSGEESCTAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFP 60
           MS EESCTAPAAVE+R  NSPGGY+VNTAVYHSL+Q DEA KIST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPINSPGGYDVNTAVYHSLVQFDEAPKISTSPDLDTATFVLSQFP 60

Query: 61  TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
           TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
           AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTG+PTILTTRP YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTTRPSYGD 180

Query: 181 VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIW 240
           VLSVEELIESCIESFE +PK +++QSDTAAILYSSGTTGTSKGVVLTHSNLISV+ELL W
Sbjct: 181 VLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
           YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLG 420
           GAAT  ITDKDA+AHPGSCGMLM RF AKIVDIETGEGLPPMKEGE+WLKSPTIMKEYL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
           NKEATEATMDKEGW+KTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 IIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
           I+DAAVIP  DEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
           SLAGKILRKDLVSQ K QQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQLK-QQVLSKL 563

[Close]
BLAST of Spg009731 vs. NCBI nr
Match: XP_038891768.1 (4-coumarate--CoA ligase-like 5 [Benincasa hispida])

HSP 1 Score: 983.0 bits (2540), Expect = 1.1e-282
Identity = 506/564 (89.72%), Postives = 533/564 (94.50%), Query Frame = 0 

Query: 1   MSGEESCTAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFP 60
           MSGEESCTAPAAVE++ +NSPGGYNV TAVYHSLIQ+DEA  ISTR DL+TATFVLSQFP
Sbjct: 1   MSGEESCTAPAAVEFQPQNSPGGYNVKTAVYHSLIQLDEAPIISTRQDLNTATFVLSQFP 60

Query: 61  TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
           TAESRVALIDSVTSSRVTYGQLS SIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
           AV+SIGAVITTANP+NTASEIGKQVRDSGAKLAVSAPEELHK+SPTGIPTILTTR  YGD
Sbjct: 121 AVMSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELHKLSPTGIPTILTTRSSYGD 180

Query: 181 VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIW 240
            LSVEELIESC ESFEPLPK E++QSD AAILYSSGTTGTSKGVVLTHSN+ISV+ELL W
Sbjct: 181 TLSVEELIESCSESFEPLPKIEVTQSDPAAILYSSGTTGTSKGVVLTHSNIISVIELLTW 240

Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
            VD++SSQ+DVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMI AIEK+K+NN
Sbjct: 241 CVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIAAIEKHKINN 300

Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKSG GSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSGDGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLG 420
           GAAT  ITDKDAKAHPGSCGMLM  F AKIVD+ETGEG+ PMKEGELWLKSPTIMK YLG
Sbjct: 361 GAATWMITDKDAKAHPGSCGMLMPSFFAKIVDVETGEGVAPMKEGELWLKSPTIMKGYLG 420

Query: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
           NKEATEAT+D+EGWLKTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATIDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 IIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
           I+DAAVIPM DEAAGQIPVACVVKA   +LTEEQVIQFVASQVA YKKVRGVRF+ +IPR
Sbjct: 481 ILDAAVIPMEDEAAGQIPVACVVKAPTSKLTEEQVIQFVASQVAAYKKVRGVRFISSIPR 540

Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
           SLAGKILRKDLVSQFKQQQLLSKL
Sbjct: 541 SLAGKILRKDLVSQFKQQQLLSKL 564

[Close]
BLAST of Spg009731 vs. NCBI nr
Match: KAG6601192.1 (4-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 982.6 bits (2539), Expect = 1.4e-282
Identity = 508/564 (90.07%), Postives = 533/564 (94.50%), Query Frame = 0 

Query: 1   MSGEESCTAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFP 60
           MS  ESCTAPAAVE+R  NSPG Y+VNTAVYHSL+Q+DEASKIST PDLDTATFVLSQFP
Sbjct: 1   MSRAESCTAPAAVEFRPMNSPGVYDVNTAVYHSLVQLDEASKISTSPDLDTATFVLSQFP 60

Query: 61  TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
           TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
           AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTGIPTILTTRP YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTRPSYGD 180

Query: 181 VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIW 240
           VLSVEELIESCIE+FE +PK +++QSDTAAILYSSGTTGTSKGVVLTHSNLISV+ELL W
Sbjct: 181 VLSVEELIESCIETFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
           YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLG 420
           GAAT  ITDKDA+AHPGSCGMLM RF AKIVDIETGEGLPPMKEGE+WLKSPTIMKEYL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
           NKEATEATMDKEGW+KTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 IIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
           I+DAAVIP  DEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
           SLAGKILRKDLVSQ K QQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQLK-QQVLSKL 563

[Close]
BLAST of Spg009731 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.1e-125
Identity = 245/539 (45.45%), Postives = 351/539 (65.12%), Query Frame = 0 

Query: 23  GYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQL 82
           GY  + ++++S     E  ++     +D  TF+ S+      ++A ID+ T   +T+ QL
Sbjct: 14  GYCKSNSIFYSK---REPVQLPQNHSVDVTTFISSR--AHHGKIAFIDAATGRHLTFPQL 73

Query: 83  SASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIG 142
             ++ S+A  L  A+G+RKGDV+ +LS NS+ +PV+CLAV+S+GA+ITT NP+NT  EI 
Sbjct: 74  WRAVDSVATCL-SAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIA 133

Query: 143 KQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGDVLSVEELIESCIESF--EPLPK 202
           KQ+ DS   LA + P+ + K++ + +P ++        +     ++ S  E    EP P 
Sbjct: 134 KQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPN 193

Query: 203 A---EMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLCFIP 262
                ++Q DTA +LYSSGTTG SKGVV +H NLI++++ ++        ++  F+C +P
Sbjct: 194 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTILSRFGTEDGEH-TFICTVP 253

Query: 263 MFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG-- 322
           MFHIYGL  F +GL   G T V++ +F    M+ AIEKY+   +P VPP+++ L+K+   
Sbjct: 254 MFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANH 313

Query: 323 --GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITITDKDAK 382
                DLSSL+ + SG APL K+V E F E +P V +  GYGLTESTG    T   ++++
Sbjct: 314 IRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESR 373

Query: 383 AHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATMDKEG 442
            + G+ GML     AKIV+ ETGE L   + GELWL+ PTIMK Y  N+EAT +T+D EG
Sbjct: 374 RY-GTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEG 433

Query: 443 WLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMVDEA 502
           WL+TGDL YIDEDGF+++VDR+KELIK+ GYQVAPAELE +LLSHPEI DAAVIP  D+ 
Sbjct: 434 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKE 493

Query: 503 AGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLV 553
           AGQ P+A VV+     L+E  V+ F+A  VAPYK++R V F+ +IP++ +GKILRKDL+
Sbjct: 494 AGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLI 544

[Close]
BLAST of Spg009731 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 448.4 bits (1152), Expect = 1.2e-124
Identity = 245/542 (45.20%), Postives = 349/542 (64.39%), Query Frame = 0 

Query: 19  NSPGGY-NVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRV 78
           NS  G+ N N+  Y     I     +   P LD  TF+ SQ      R+A ID+ T   +
Sbjct: 5   NSRSGFCNSNSTFYSKRTPI----PLPPNPSLDVTTFISSQ--AHRGRIAFIDASTGQNL 64

Query: 79  TYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNT 138
           T+ +L  ++ S+A  L   +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+NT
Sbjct: 65  TFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNT 124

Query: 139 ASEIGKQVRDSGAKLAVSAPEELHKVSPTG--IPTILTTRPPYGDVLSVEELIESC-IES 198
           ++EI KQ++DS   LA +  + L K+S     +P +L        V  V  L+E    E 
Sbjct: 125 SNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEP 184

Query: 199 FEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLC 258
                K  + Q DTA +LYSSGTTG SKGV+ +H NLI++++ ++    +   +   F+C
Sbjct: 185 SGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR-FIC 244

Query: 259 FIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKS 318
            +PMFHIYGL  F  GL   G T +++ +F    M+ AI KY+  ++P VPP+++ +V  
Sbjct: 245 TVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNG 304

Query: 319 G----GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITITDK 378
                   DLSS+  +  G APL K+V E F EK+P V++  GYGLTESTG    T T +
Sbjct: 305 ADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVE 364

Query: 379 DAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATMD 438
           +++ + G+ G L +    +IVD  TG+ L P + GELWLK P+IMK Y  N+EAT +T+D
Sbjct: 365 ESRRY-GTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLD 424

Query: 439 KEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMV 498
            EGWL+TGDL YIDEDGF+++VDR+KELIK+ GYQVAPAELE +LL+HPEI DAAVIP  
Sbjct: 425 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFP 484

Query: 499 DEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKD 553
           D+  GQ P+A VV+ +   L+E+ +++FVA QVAPYK++R V F+ +IP++ +GKILRKD
Sbjct: 485 DKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKD 537

[Close]
BLAST of Spg009731 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 5.4e-120
Identity = 234/522 (44.83%), Postives = 334/522 (63.98%), Query Frame = 0 

Query: 47  PDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVF 106
           P+    +F+     +  S++A+ DS T   +T+ QL +++  LA G +H LG+RK DVV 
Sbjct: 25  PNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG-FHRLGIRKNDVVL 84

Query: 107 VLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPT 166
           + + NS  +P+  LAV +IG V TTANP+ T +E+ KQ++DS  K+ +S  +   K+   
Sbjct: 85  IFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGF 144

Query: 167 GIPTIL-----TTRPPYGD---VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTT 226
            +P +L     T   P G    +LS + ++E   E     P  E+ QSDTAA+LYSSGTT
Sbjct: 145 DLPVVLLGSKDTVEIPPGSNSKILSFDNVME-LSEPVSEYPFVEIKQSDTAALLYSSGTT 204

Query: 227 GTSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITT 286
           GTSKGV LTH N I+   ++    D     + VFLCF+PMFH++GL         RG   
Sbjct: 205 GTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNAL 264

Query: 287 VLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG--GGSDLSSLRRIGSGAAPLGKD 346
           V M RF  + ++  IEK++V ++  VPPV L L K       DLSSL+ IGSGAAPLGKD
Sbjct: 265 VSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGKD 324

Query: 347 VEEAFREKFPWVELRPGYGLTESTGAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETG 406
           + E      P V L  GYG+TE+ G  ++    +  K + GS GML     A+IV +ETG
Sbjct: 325 LMEECGRNIPNVLLMQGYGMTETCGIVSVE-DPRLGKRNSGSAGMLAPGVEAQIVSVETG 384

Query: 407 EGLPPMKEGELWLKSPTIMKEYLGNKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIK 466
           +  PP ++GE+W++ P +MK YL N +AT+ T+DK+ W+ TGDLGY +EDG +Y+VDRIK
Sbjct: 385 KSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIK 444

Query: 467 ELIKHNGYQVAPAELETILLSHPEIIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVI 526
           ELIK+ G+QVAPAELE +L+SHP+I+DA VIP  DE AG++P+A VV++ N  +TE+ + 
Sbjct: 445 ELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQ 504

Query: 527 QFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLVSQFKQQ 559
           +F+A QVAPYK++R V F+  +P+S AGKILR++LV Q + +
Sbjct: 505 KFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSK 543

[Close]
BLAST of Spg009731 vs. ExPASy Swiss-Prot
Match: P0C5B6 (OPC-6:CoA ligase OS=Arabidopsis thaliana OX=3702 GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 3.8e-118
Identity = 230/521 (44.15%), Postives = 334/521 (64.11%), Query Frame = 0 

Query: 43  ISTRPDL--DTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVR 102
           +S  P+L  D  TF+ SQ      + A ID+ T   +T+  L  ++  +A  LYH +G+R
Sbjct: 30  LSLPPNLSRDVTTFISSQ--PHRGKTAFIDAATGQCLTFSDLWRAVDRVADCLYHEVGIR 89

Query: 103 KGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEEL 162
           +GDVV +LS NS+  PV+CL+V+S+GAV TTAN +NT+ EI KQ+ DS   L V    +L
Sbjct: 90  RGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQIADSNPTL-VFTTRQL 149

Query: 163 HKVSPTGIPTILTTRPPYGDVLSVEELIESCIESFEPLPKAE-----MSQSDTAAILYSS 222
               P  I  +LT    Y ++ S   ++    E  +  P  +     ++Q DTA +LYSS
Sbjct: 150 APKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRVRDRVNQDDTAMMLYSS 209

Query: 223 GTTGTSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRG 282
           GTTG SKGV+ +H NL + +     ++  +  ++D+F+C +PMFH YGL+ F +G    G
Sbjct: 210 GTTGPSKGVISSHRNLTAHVAR---FISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALG 269

Query: 283 ITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG----GGSDLSSLRRIGSGAA 342
            T V+++RF    M+DA+EK++   +   PPV++ ++          DLSSL+ +  G A
Sbjct: 270 STVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGA 329

Query: 343 PLGKDVEEAFREKFPWVELRPGYGLTESTGAATITITDKDAKAHPGSCGMLMSRFSAKIV 402
           PL K+V E F EK+P V++  GY LTES G    T + ++++ + G+ G L S   A+IV
Sbjct: 330 PLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESRRY-GTAGTLTSDVEARIV 389

Query: 403 DIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATMDKEGWLKTGDLGYIDEDGFVYI 462
           D  TG  +   + GELWLK P+I K Y  N+EAT  T++ EGWLKTGDL YIDEDGF+++
Sbjct: 390 DPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGWLKTGDLCYIDEDGFLFV 449

Query: 463 VDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMVDEAAGQIPVACVVKASNCQLT 522
           VDR+KELIK+ GYQV PAELE +L++HP+I+DAAVIP  D+ AGQ P+A VV+     L+
Sbjct: 450 VDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLS 509

Query: 523 EEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLV 553
           E+QVI F++ QVAPYKK+R V F+++IP++ +GK LRKDL+
Sbjct: 510 EKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLI 543

[Close]
BLAST of Spg009731 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 3.3e-117
Identity = 230/519 (44.32%), Postives = 334/519 (64.35%), Query Frame = 0 

Query: 48  DLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFV 107
           +L   +FV     +   + ALIDS T+  +++ Q  + +  ++ G  + LGV+K DVV +
Sbjct: 26  NLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQFKSMVIKVSHGFLN-LGVQKNDVVLI 85

Query: 108 LSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTG 167
            + NS+  PV  L +++ GA+ TT+NP+ T SE+ KQV+DS  KL V+ PE   KV    
Sbjct: 86  FAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSKQVKDSNPKLIVTVPELFEKVKGFN 145

Query: 168 IPTIL--------TTRPPYGDVLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTG 227
           +PTIL        +       VL+  +L+ +        P  +  QSDTAA+LYSSGTTG
Sbjct: 146 LPTILIGPNSEDSSPLKSRAKVLTFHDLV-TLSGPVSDFPMVDFKQSDTAALLYSSGTTG 205

Query: 228 TSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTV 287
            SKGVVL+H N I+   ++    D +   ++VFLCF+PMFH++GL         RG T +
Sbjct: 206 MSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLCFLPMFHVFGLAIITYAQLQRGNTVI 265

Query: 288 LMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG--GGSDLSSLRRIGSGAAPLGKDV 347
            M RF+ + ++  +EKYKV ++  VPPVIL L K+      DLSSL+ IGSGAAPLGKD+
Sbjct: 266 SMARFDLEKILKDVEKYKVTHLWVVPPVILALTKNSIVKKYDLSSLKHIGSGAAPLGKDL 325

Query: 348 EEAFREKFPWVELRPGYGLTESTGAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGE 407
            E   +  P   +  GYG+TE+ G  ++  T +  K H GS GML S   A+IV ++T +
Sbjct: 326 MEECAKIVPHGIVAQGYGMTETCGIVSVEDT-RGGKRHTGSAGMLSSGVEAQIVSVDTLK 385

Query: 408 GLPPMKEGELWLKSPTIMKEYLGNKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKE 467
            LPP + GE+W++ P +M+ Y  N  AT+ T+DK+GW+ TGDLGY DEDG +Y+VDRIKE
Sbjct: 386 PLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKGWVHTGDLGYFDEDGHLYVVDRIKE 445

Query: 468 LIKHNGYQVAPAELETILLSHPEIIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQ 527
           LIK+ G+QVAPAELE +L+SHPEI+DA VIP  D  AG++PVA VV++ N  LTE+ V +
Sbjct: 446 LIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDADAGEVPVAYVVRSPNSSLTEDDVKK 505

Query: 528 FVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLVSQFK 557
           F+A QVA +K++R V F++++P+S +GKILR++L+ + +
Sbjct: 506 FIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQKVR 541

[Close]
BLAST of Spg009731 vs. ExPASy TrEMBL
Match: A0A6J1K303 (4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.5e-285
Identity = 509/564 (90.25%), Postives = 534/564 (94.68%), Query Frame = 0 

Query: 1   MSGEESCTAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFP 60
           MS EESCTAPAAVE+R  NSPGGY+VNTAVYHS +Q+DEA KIST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPMNSPGGYDVNTAVYHSFVQLDEAPKISTSPDLDTATFVLSQFP 60

Query: 61  TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
           TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
           AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTGIPTILTT+P YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTTQPSYGD 180

Query: 181 VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIW 240
           VLSVEELIESCIESFE +P+ +++QSD AAILYSSGTTGTSKGVVLTHSNLISV+ELL W
Sbjct: 181 VLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
           YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLG 420
           GAAT  ITDKDA+AHPGSCGMLM RF AKIVDIETGEGLPPMKEGE+WLKSPTIMKEYL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
           NKEATEATMDKEGWLKTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 IIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
           I+DAAVIP  DEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
           SLAGKILRKDLVSQFKQQQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQFKQQQVLSKL 564

[Close]
BLAST of Spg009731 vs. ExPASy TrEMBL
Match: A0A6J1H2E6 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=4 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 7.9e-284
Identity = 509/564 (90.25%), Postives = 533/564 (94.50%), Query Frame = 0 

Query: 1   MSGEESCTAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFP 60
           MS EESCTAPAAVE+R  NSPGGY+VNTAVYHSL+Q DEA KIST PDLDTATFVLSQFP
Sbjct: 1   MSREESCTAPAAVEFRPINSPGGYDVNTAVYHSLVQFDEAPKISTSPDLDTATFVLSQFP 60

Query: 61  TAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICL 120
           TAESRVALIDSVTSSRVTYG+LSAS RSLA GLYHALGVRKGDVVFVLSANSVLYPVICL
Sbjct: 61  TAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVLYPVICL 120

Query: 121 AVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGD 180
           AVLSIGAVITTANPVNTASEIGKQV DS AKLA+SAPEELHK+SPTG+PTILTTRP YGD
Sbjct: 121 AVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTTRPSYGD 180

Query: 181 VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIW 240
           VLSVEELIESCIESFE +PK +++QSDTAAILYSSGTTGTSKGVVLTHSNLISV+ELL W
Sbjct: 181 VLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTW 240

Query: 241 YVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNN 300
           YVDASSSQ+DVFLCF+PMFHIYGLVFFGLGLFCRG T VLMQRFNFQSMIDAIE YKVNN
Sbjct: 241 YVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAIETYKVNN 300

Query: 301 IPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360
           IPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST
Sbjct: 301 IPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTEST 360

Query: 361 GAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLG 420
           GAAT  ITDKDA+AHPGSCGMLM RF AKIVDIETGEGLPPMKEGE+WLKSPTIMKEYL 
Sbjct: 361 GAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPTIMKEYLR 420

Query: 421 NKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPE 480
           NKEATEATMDKEGW+KTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH E
Sbjct: 421 NKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTE 480

Query: 481 IIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPR 540
           I+DAAVIP  DEAAGQIPVACVVKA +C+LTEEQVIQFVASQVA YKK+RGVRFM +IPR
Sbjct: 481 ILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVRFMSSIPR 540

Query: 541 SLAGKILRKDLVSQFKQQQLLSKL 565
           SLAGKILRKDLVSQ K QQ+LSKL
Sbjct: 541 SLAGKILRKDLVSQLK-QQVLSKL 563

[Close]
BLAST of Spg009731 vs. ExPASy TrEMBL
Match: A0A6J1CEK1 (4-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE=4 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.5e-279
Identity = 504/571 (88.27%), Postives = 534/571 (93.52%), Query Frame = 0 

Query: 1   MSGEESCTAPAAVEYRRRNSP------GGYNVNTAVYHSLIQIDEASKISTRPDLDTATF 60
           MSGEES TAPAA EY R+N+P      GGY+VN AVYHSL++ DEA KIS+RPDLDTATF
Sbjct: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNIAVYHSLVKFDEARKISSRPDLDTATF 60

Query: 61  VLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVL 120
           VLSQFP AESRVALIDSVTSSRVTYGQLSASIRSLACGL+HALGVRKGDVVFVLSANS+L
Sbjct: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120

Query: 121 YPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTT 180
           YPVICLAVLS+GAVITTANPVNTASEI KQVRDSGAKLAVSAPEE+HK++PTGIPTILTT
Sbjct: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180

Query: 181 RPPYG-DVLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           RP  G DVLSVEELIESC +S E LP  E++QSDTAAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAI 300
           V+ELL  YVDASSS+NDVFLCFIPMFHIYGLVFFGLGLFCRGIT +LMQRFNFQ+MIDAI
Sbjct: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPG 360
           EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAAT  ITD DAK HPGSCGMLM RFSAKIVDIETGEGLPPMKEGELWLKSPT
Sbjct: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420

Query: 421 IMKEYLGNKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELET 480
           IMK YLGN+EATEATMD+EGWLKTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILLSHPEIIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVR 540
           IL+ H EI+DAAVIPM DEAAGQIPVACVVKAS  QLTE+QVIQFVASQVAPYKKVRGV+
Sbjct: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540

Query: 541 FMDAIPRSLAGKILRKDLVSQFKQQQLLSKL 565
           F++AIPRSLAGKILRKDLVSQFKQQQL SKL
Sbjct: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571

[Close]
BLAST of Spg009731 vs. ExPASy TrEMBL
Match: A0A1S3BFZ3 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489425 PE=4 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 2.0e-279
Identity = 502/566 (88.69%), Postives = 528/566 (93.29%), Query Frame = 0 

Query: 1   MSGEESC-TAPAAVEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQF 60
           MS EESC T PAA+E++ RNSPGGYNVNTAVYHSLI  DEA  ISTR DLDTAT+VLSQF
Sbjct: 1   MSDEESCSTGPAAMEFQPRNSPGGYNVNTAVYHSLIHFDEAPVISTRSDLDTATYVLSQF 60

Query: 61  PTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVIC 120
           PTAESRVALIDSVTS RVTYGQL  SIRSLACGLYHALGVRKGDVVFVLSAN VLYPVIC
Sbjct: 61  PTAESRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVIC 120

Query: 121 LAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYG 180
           LAVLSIGAVITTANP+NTASEIGKQVRDSGAKLAVSAPEEL K+ PTGIPTILTTR  + 
Sbjct: 121 LAVLSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTILTTRSSFS 180

Query: 181 DVLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLI 240
           D LSVEELIESC ES EPLPK E++QSDTAAILYSSGTTGTSKGVVLTHSNLISV+ELL 
Sbjct: 181 DALSVEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLT 240

Query: 241 WYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVN 300
           W VD++SSQ+DVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYK+N
Sbjct: 241 WCVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKIN 300

Query: 301 NIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES 360
           NIPAVPPVILGLVKSGGGSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTES
Sbjct: 301 NIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTES 360

Query: 361 TGAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYL 420
           TGAAT  ITDKDAKAHPGSCGMLM  F  KIVD+ETGEGLPPMK+GELWLKSPTIMKEYL
Sbjct: 361 TGAATWMITDKDAKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYL 420

Query: 421 GNKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHP 480
           GN+EATEAT+D+EGWLKTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH 
Sbjct: 421 GNREATEATLDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHT 480

Query: 481 EIIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIP 540
           EI+DAAVIP+ DEAAGQIPVACVVKA +C LTE+QVIQFVASQVAPYKKVRGVRF+ AIP
Sbjct: 481 EILDAAVIPIEDEAAGQIPVACVVKAPSCVLTEQQVIQFVASQVAPYKKVRGVRFISAIP 540

Query: 541 RSLAGKILRKDLVSQFK-QQQLLSKL 565
           RSLAGKILRKDLVSQFK QQQL SKL
Sbjct: 541 RSLAGKILRKDLVSQFKQQQQLFSKL 566

[Close]
BLAST of Spg009731 vs. ExPASy TrEMBL
Match: A0A0A0KRI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622450 PE=4 SV=1)

HSP 1 Score: 946.0 bits (2444), Expect = 7.0e-272
Identity = 488/553 (88.25%), Postives = 519/553 (93.85%), Query Frame = 0 

Query: 13  VEYRRRNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSV 72
           VE++ ++SPGGYNVNTAVYHSL+ +DEA  ISTR DLDTAT+VLSQFPTAESRVALIDSV
Sbjct: 7   VEFQPQSSPGGYNVNTAVYHSLLHLDEAPVISTRSDLDTATYVLSQFPTAESRVALIDSV 66

Query: 73  TSSRVTYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTA 132
           TS RVTYGQLS SIRSLACGLYHALGVRKGDVVFVLSAN VLYPVICLAVLSIGAVITTA
Sbjct: 67  TSLRVTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVICLAVLSIGAVITTA 126

Query: 133 NPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGDVLSVEELIESCI 192
           NP+NT SEIGKQVRDSGAKLAVSAPEEL K+SPTGIPTILTTR  YGD LSVEELIESC 
Sbjct: 127 NPMNTESEIGKQVRDSGAKLAVSAPEELQKLSPTGIPTILTTRSSYGDALSVEELIESCS 186

Query: 193 E-SFEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIWYVDASSSQNDV 252
           E S EPLPKAE++ SDTAAILYSSGTTGTSKGVVLTHSNLISV+E+L W VD++SSQ+DV
Sbjct: 187 ESSSEPLPKAEVTPSDTAAILYSSGTTGTSKGVVLTHSNLISVIEILTWCVDSTSSQHDV 246

Query: 253 FLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGL 312
           FLCFIPMFHIYGLVFFGLGLF RGITTVLM RFNFQSMIDAIEKYK+NNIPAVPPVILGL
Sbjct: 247 FLCFIPMFHIYGLVFFGLGLFRRGITTVLMPRFNFQSMIDAIEKYKINNIPAVPPVILGL 306

Query: 313 VKSGGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITITDKD 372
           VKS GGSD SSLRR+GSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAAT  ITDKD
Sbjct: 307 VKSDGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMITDKD 366

Query: 373 AKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATMDK 432
           AKAHPGSCGMLM  F  KIVD+ETGEGLPPMK+GELWLKSPTIMKEYLGN+EATEAT+D+
Sbjct: 367 AKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYLGNREATEATIDE 426

Query: 433 EGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMVD 492
           EGWLKTGDLGYIDEDGF+YIVDRIKELIKHNGYQVAPAELETILLSH EI+DAAVIP+ D
Sbjct: 427 EGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTEILDAAVIPIED 486

Query: 493 EAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDL 552
           EAAGQIPVACVVKA +C+L+E+QVIQFVASQVAPYKKVRGVRF+ AIPRSLAGKILRKDL
Sbjct: 487 EAAGQIPVACVVKAPSCELSEQQVIQFVASQVAPYKKVRGVRFISAIPRSLAGKILRKDL 546

Query: 553 VSQFKQQQLLSKL 565
           VSQFKQ QLLSKL
Sbjct: 547 VSQFKQLQLLSKL 559

[Close]
BLAST of Spg009731 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 448.4 bits (1152), Expect = 8.8e-126
Identity = 245/542 (45.20%), Postives = 349/542 (64.39%), Query Frame = 0 

Query: 19  NSPGGY-NVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRV 78
           NS  G+ N N+  Y     I     +   P LD  TF+ SQ      R+A ID+ T   +
Sbjct: 5   NSRSGFCNSNSTFYSKRTPI----PLPPNPSLDVTTFISSQ--AHRGRIAFIDASTGQNL 64

Query: 79  TYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNT 138
           T+ +L  ++ S+A  L   +G+RKG VV +LS NS+L+PV+CL+V+S+GA+ITT NP+NT
Sbjct: 65  TFTELWRAVESVADCL-SEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNT 124

Query: 139 ASEIGKQVRDSGAKLAVSAPEELHKVSPTG--IPTILTTRPPYGDVLSVEELIESC-IES 198
           ++EI KQ++DS   LA +  + L K+S     +P +L        V  V  L+E    E 
Sbjct: 125 SNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEP 184

Query: 199 FEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLC 258
                K  + Q DTA +LYSSGTTG SKGV+ +H NLI++++ ++    +   +   F+C
Sbjct: 185 SGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR-FIC 244

Query: 259 FIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKS 318
            +PMFHIYGL  F  GL   G T +++ +F    M+ AI KY+  ++P VPP+++ +V  
Sbjct: 245 TVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNG 304

Query: 319 G----GGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITITDK 378
                   DLSS+  +  G APL K+V E F EK+P V++  GYGLTESTG    T T +
Sbjct: 305 ADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVE 364

Query: 379 DAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATMD 438
           +++ + G+ G L +    +IVD  TG+ L P + GELWLK P+IMK Y  N+EAT +T+D
Sbjct: 365 ESRRY-GTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLD 424

Query: 439 KEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMV 498
            EGWL+TGDL YIDEDGF+++VDR+KELIK+ GYQVAPAELE +LL+HPEI DAAVIP  
Sbjct: 425 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFP 484

Query: 499 DEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKD 553
           D+  GQ P+A VV+ +   L+E+ +++FVA QVAPYK++R V F+ +IP++ +GKILRKD
Sbjct: 485 DKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKD 537

[Close]
BLAST of Spg009731 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 433.0 bits (1112), Expect = 3.8e-121
Identity = 234/522 (44.83%), Postives = 334/522 (63.98%), Query Frame = 0 

Query: 47  PDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVRKGDVVF 106
           P+    +F+     +  S++A+ DS T   +T+ QL +++  LA G +H LG+RK DVV 
Sbjct: 25  PNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG-FHRLGIRKNDVVL 84

Query: 107 VLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEELHKVSPT 166
           + + NS  +P+  LAV +IG V TTANP+ T +E+ KQ++DS  K+ +S  +   K+   
Sbjct: 85  IFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGF 144

Query: 167 GIPTIL-----TTRPPYGD---VLSVEELIESCIESFEPLPKAEMSQSDTAAILYSSGTT 226
            +P +L     T   P G    +LS + ++E   E     P  E+ QSDTAA+LYSSGTT
Sbjct: 145 DLPVVLLGSKDTVEIPPGSNSKILSFDNVME-LSEPVSEYPFVEIKQSDTAALLYSSGTT 204

Query: 227 GTSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRGITT 286
           GTSKGV LTH N I+   ++    D     + VFLCF+PMFH++GL         RG   
Sbjct: 205 GTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAVITYSQLQRGNAL 264

Query: 287 VLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG--GGSDLSSLRRIGSGAAPLGKD 346
           V M RF  + ++  IEK++V ++  VPPV L L K       DLSSL+ IGSGAAPLGKD
Sbjct: 265 VSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKFDLSSLKYIGSGAAPLGKD 324

Query: 347 VEEAFREKFPWVELRPGYGLTESTGAATITITDKDAKAHPGSCGMLMSRFSAKIVDIETG 406
           + E      P V L  GYG+TE+ G  ++    +  K + GS GML     A+IV +ETG
Sbjct: 325 LMEECGRNIPNVLLMQGYGMTETCGIVSVE-DPRLGKRNSGSAGMLAPGVEAQIVSVETG 384

Query: 407 EGLPPMKEGELWLKSPTIMKEYLGNKEATEATMDKEGWLKTGDLGYIDEDGFVYIVDRIK 466
           +  PP ++GE+W++ P +MK YL N +AT+ T+DK+ W+ TGDLGY +EDG +Y+VDRIK
Sbjct: 385 KSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIK 444

Query: 467 ELIKHNGYQVAPAELETILLSHPEIIDAAVIPMVDEAAGQIPVACVVKASNCQLTEEQVI 526
           ELIK+ G+QVAPAELE +L+SHP+I+DA VIP  DE AG++P+A VV++ N  +TE+ + 
Sbjct: 445 ELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQ 504

Query: 527 QFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLVSQFKQQ 559
           +F+A QVAPYK++R V F+  +P+S AGKILR++LV Q + +
Sbjct: 505 KFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQQVRSK 543

[Close]
BLAST of Spg009731 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 426.8 bits (1096), Expect = 2.7e-119
Identity = 230/521 (44.15%), Postives = 334/521 (64.11%), Query Frame = 0 

Query: 43  ISTRPDL--DTATFVLSQFPTAESRVALIDSVTSSRVTYGQLSASIRSLACGLYHALGVR 102
           +S  P+L  D  TF+ SQ      + A ID+ T   +T+  L  ++  +A  LYH +G+R
Sbjct: 30  LSLPPNLSRDVTTFISSQ--PHRGKTAFIDAATGQCLTFSDLWRAVDRVADCLYHEVGIR 89

Query: 103 KGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIGKQVRDSGAKLAVSAPEEL 162
           +GDVV +LS NS+  PV+CL+V+S+GAV TTAN +NT+ EI KQ+ DS   L V    +L
Sbjct: 90  RGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQIADSNPTL-VFTTRQL 149

Query: 163 HKVSPTGIPTILTTRPPYGDVLSVEELIESCIESFEPLPKAE-----MSQSDTAAILYSS 222
               P  I  +LT    Y ++ S   ++    E  +  P  +     ++Q DTA +LYSS
Sbjct: 150 APKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRVRDRVNQDDTAMMLYSS 209

Query: 223 GTTGTSKGVVLTHSNLISVMELLIWYVDASSSQNDVFLCFIPMFHIYGLVFFGLGLFCRG 282
           GTTG SKGV+ +H NL + +     ++  +  ++D+F+C +PMFH YGL+ F +G    G
Sbjct: 210 GTTGPSKGVISSHRNLTAHVAR---FISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALG 269

Query: 283 ITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLVKSG----GGSDLSSLRRIGSGAA 342
            T V+++RF    M+DA+EK++   +   PPV++ ++          DLSSL+ +  G A
Sbjct: 270 STVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGA 329

Query: 343 PLGKDVEEAFREKFPWVELRPGYGLTESTGAATITITDKDAKAHPGSCGMLMSRFSAKIV 402
           PL K+V E F EK+P V++  GY LTES G    T + ++++ + G+ G L S   A+IV
Sbjct: 330 PLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESRRY-GTAGTLTSDVEARIV 389

Query: 403 DIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEATMDKEGWLKTGDLGYIDEDGFVYI 462
           D  TG  +   + GELWLK P+I K Y  N+EAT  T++ EGWLKTGDL YIDEDGF+++
Sbjct: 390 DPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGWLKTGDLCYIDEDGFLFV 449

Query: 463 VDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIPMVDEAAGQIPVACVVKASNCQLT 522
           VDR+KELIK+ GYQV PAELE +L++HP+I+DAAVIP  D+ AGQ P+A VV+     L+
Sbjct: 450 VDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLS 509

Query: 523 EEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILRKDLV 553
           E+QVI F++ QVAPYKK+R V F+++IP++ +GK LRKDL+
Sbjct: 510 EKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLI 543

[Close]
BLAST of Spg009731 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 414.5 bits (1064), Expect = 1.4e-115
Identity = 237/543 (43.65%), Postives = 345/543 (63.54%), Query Frame = 0 

Query: 23  GYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRVTYGQL 82
           G+  +T++++S     E   +     LD  +F+ SQ      +   +D+VT  R+++ +L
Sbjct: 26  GFCESTSIFYSK---REPMALPPNQFLDVTSFIASQ--PHRGKTVFVDAVTGRRLSFPEL 85

Query: 83  SASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNTASEIG 142
              +  +A G  +ALGVRKG+VV +LS NS+L+P++ L+V+S+GA+ITTANP+NT+ EI 
Sbjct: 86  WLGVERVA-GCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 145

Query: 143 KQVRDSGAKLAVSAPEELHKV---SPTGIPTIL-----TTRPPYGDVLSVEELIESCIES 202
           KQ+ DS   LA +  + + K+   S   +P +L          YGD + +   +E+ IE+
Sbjct: 146 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 205

Query: 203 --FEPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIWYVDASSSQNDVF 262
              E   K  ++Q DTAA+LYSSGTTGTSKGV+L+H NLI+    L+    A        
Sbjct: 206 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA----LVQAYRARFGLEQRT 265

Query: 263 LCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGLV 322
           +C IPM HI+G   F  GL   G T V++ +F+   ++ A+E ++ + +  VPP+++ +V
Sbjct: 266 ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 325

Query: 323 KS----GGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIT 382
                     DLSSL  + +G APL ++V E F E +P V++  GYGLTEST  A     
Sbjct: 326 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 385

Query: 383 DKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEAT 442
            ++ K + G+ G+L      KIVD +TG  L   + GELW++SPT+MK Y  NKEAT +T
Sbjct: 386 KEETKRY-GASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATAST 445

Query: 443 MDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIP 502
           +D EGWLKTGDL YID DGFV++VDR+KELIK NGYQVAPAELE +LL+HPEI DAAVIP
Sbjct: 446 IDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIP 505

Query: 503 MVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILR 552
           + D  AGQ P+A +V+     L+E +++ FVA QV+PYKK+R V F+ +IP++ +GKILR
Sbjct: 506 IPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILR 557

[Close]
BLAST of Spg009731 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 414.5 bits (1064), Expect = 1.4e-115
Identity = 230/543 (42.36%), Postives = 344/543 (63.35%), Query Frame = 0 

Query: 18  RNSPGGYNVNTAVYHSLIQIDEASKISTRPDLDTATFVLSQFPTAESRVALIDSVTSSRV 77
           +N P  ++  T +Y S      +  +   P+LD  + + S     ++  ALIDS+T   +
Sbjct: 19  QNPPFWFSSKTGIYTSKF---PSLHLPVDPNLDAVSALFSHKHHGDT--ALIDSLTGFSI 78

Query: 78  TYGQLSASIRSLACGLYHALGVRKGDVVFVLSANSVLYPVICLAVLSIGAVITTANPVNT 137
           ++ +L   ++S+A G+YH LGVR+GDVV ++  NSV +P+I L+++S+GA++TT NP ++
Sbjct: 79  SHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSS 138

Query: 138 ASEIGKQVRDSGAKLAVSAPEELHKVSPTGIPTILTTRPPYGDVLSVE--ELIESCIESF 197
             EI KQV +    LA ++ E + K+S  G+  I  +     D + +E  +      ESF
Sbjct: 139 LGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESF 198

Query: 198 EPLPKAEMSQSDTAAILYSSGTTGTSKGVVLTHSNLISVMELLIWYVDASS----SQNDV 257
             +PK  + Q D AAI+YSSGTTG SKGV+LTH NLI+ MEL + + +AS       ++V
Sbjct: 199 GFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRF-EASQYEYPGSSNV 258

Query: 258 FLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDAIEKYKVNNIPAVPPVILGL 317
           +L  +P+ HIYGL  F +GL   G T V+M+RF+   +++ IE++K+ + P VPP+++ L
Sbjct: 259 YLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMAL 318

Query: 318 VKSGG---GSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATITIT 377
            K      G    SL+++ SGAAPL +   E F +  P V+L  GYG+TEST   T    
Sbjct: 319 TKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFN 378

Query: 378 DKDAKAHPGSCGMLMSRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKEYLGNKEATEAT 437
            +    +  S G+L     AK+VD  +G  LPP   GELW++ P +MK YL N +AT+ +
Sbjct: 379 SEKLSRY-SSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMS 438

Query: 438 MDKEGWLKTGDLGYIDEDGFVYIVDRIKELIKHNGYQVAPAELETILLSHPEIIDAAVIP 497
           + ++ WL+TGD+ Y DEDG+++IVDRIKE+IK+ G+Q+APA+LE +L+SHP IIDAAV  
Sbjct: 439 IVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTA 498

Query: 498 MVDEAAGQIPVACVVKASNCQLTEEQVIQFVASQVAPYKKVRGVRFMDAIPRSLAGKILR 552
             +E  G+IPVA VV+     L+EE VI +VASQVAPY+KVR V  +++IP+S  GKILR
Sbjct: 499 APNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGKILR 554

The following BLAST results are available for this feature:
BLAST of Spg009731 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022994494.15.1e-28590.254-coumarate--CoA ligase-like 5 [Cucurbita maxima][more]
KAG7031989.12.5e-28490.434-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022957594.11.6e-28390.254-coumarate--CoA ligase 1-like [Cucurbita moschata][more]
XP_038891768.11.1e-28289.724-coumarate--CoA ligase-like 5 [Benincasa hispida][more]
KAG6601192.11.4e-28290.074-coumarate--CoA ligase-like 5, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of Spg009731 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
M4IQR71.1e-12545.45Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1[more]
Q84P211.2e-12445.204-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=... [more]
Q9M0X95.4e-12044.834-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=... [more]
P0C5B63.8e-11844.15OPC-6:CoA ligase OS=Arabidopsis thaliana OX=3702 GN=4CLL4 PE=2 SV=1[more]
M4IRL63.3e-11744.32Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1[more]
BLAST of Spg009731 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1K3032.5e-28590.254-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 ... [more]
A0A6J1H2E67.9e-28490.254-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=... [more]
A0A6J1CEK11.5e-27988.274-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE... [more]
A0A1S3BFZ32.0e-27988.694-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348942... [more]
A0A0A0KRI47.0e-27288.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622450 PE=4 SV=1[more]
BLAST of Spg009731 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G20510.18.8e-12645.20OPC-8:0 CoA ligase1 [more]
AT4G05160.13.8e-12144.83AMP-dependent synthetase and ligase family protein [more]
AT1G20500.12.7e-11944.15AMP-dependent synthetase and ligase family protein [more]
AT1G20480.11.4e-11543.65AMP-dependent synthetase and ligase family protein [more]
AT4G19010.11.4e-11542.36AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.30coord: 457..561
e-value: 4.0E-29
score: 103.3
NoneNo IPR availablePANTHERPTHR24096:SF376AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN-RELATEDcoord: 16..557
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 16..557
NoneNo IPR availableCDDcd059044CLcoord: 43..551
e-value: 0.0
score: 754.456
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 58..557
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 470..545
e-value: 2.5E-13
score: 50.8
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 35..456
e-value: 4.8E-125
score: 419.6
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 63..460
e-value: 2.4E-99
score: 332.8
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 211..222

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009731.1Spg009731.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009698 phenylpropanoid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016207 4-coumarate-CoA ligase activity