Spg009657 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009657
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein arginine N-methyltransferase
Locationscaffold7: 6788189 .. 6792491 (+)
RNA-Seq ExpressionSpg009657
SyntenySpg009657
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGACAATAAAAAAAAATAGTCATGTATTTTCAATCAAAATGTGAAAAGTTGAAATTTCCAACAAGAGCATAGCTCAACGGGAATAAAGTTGTATTGATAATCACGAGGTATGTGGTTCGAATCCCCACCCCCATATTGTCAAAAAAAAAAAAAAAAAAAAAAAATCAAAATGTGAAAAGTTGAAATTTCCACCACATATTTAAAAATTTAAAAAGTAATAATAATAAATAGTCACCTTAGATAAAAAGTATTTATAAACCAATAATTACGACCTTATTATAATGTCGTCCATTCCTCACAAAAGAAAAAACCCTGGCAACTCTGCTTGGCTGATAGGGTTTAAGCATCGCCGGCAAGGCAACTGATAACCAAACACTGCCACTCTGTTTCCTCTTTGACATACACCATGGATCAATTTCCCAAAGAACTCGACAAAGAATTCTGCAACACCGAGAAAATTGAAAGAGAAGAAGAAGAAGACGACGAGGACGACGAGCAAGAAGATGGACAAGACGACTGGGACGATTGGAATGCAGACGAGGAAGAAGGCGAAGAAGCATTTGACTCGAATTTTCTGTGTCTGTTCTGCAACTCCAAGTATAGTGACTGCGACACGCTGTTCAGACATTGCAACTCGGTCCACTACTTCGATTTTCATGACATTAGGAGGGCTCTGGGTTTAGACTTTTATGGTTCGTTTAAAATCATCAACTACATACGATCTCAGGTTAGCCAAGTTTGTCAGAGAATTTTGATTTTTGTGAATTTCATGTTTGTTGTCAAAATGGTCCAGAAAAGAGATGGCTTTTTATGCACTTAAAGTTGGGAGCTGCTCTAATTAGCTGGACTGGATCGATGGAGTGTCCGTGTTTCAGCGTTTGGTTTCCCCAAGATATTTTTTGTAGAATTTCTTTTGGCCAGCATTTGAAACGTTACCTCACACTTATGACTGTCCAATTGGGTTGGGGGGGGGGGGGGATTGGAGATATTGTTGTCACGATGATTTGATTTGAAGTTTTAGTTTACAAATCTTATTAGCTACGAAACAAGCGTTGACACGTTATTTACCACTTCTGGAACTGACCAGGTAGCAGAAAATAGGTGTTGGAGCTGTGGTCTCTTGTATCAATCAAACCAGGATTTACAGAACCATTTACACAAAACATTCAATCTTAGTGACATCAAGGCTCTTTGGGATGATGATAAATATCTAAAACCCTTCAGGCAAGATGATCCCCTCCTGTACAGTTTTGCAGAAGACGAAGAGGATGATGACATTTCACTCATGAACAATACGGATACTGATGGAGGTAATTGCAATGTTGATGAAATGACTGGAGAAAGTCTTCCATTTCATCCAAACCGACACAAAGAAAATGGAATGGAAGATTGTAGTTCTACTTCACACAGTTTTTCAAATGCTCCAAGAAACTTAGAGGCCGTGATTGAGAATACCGTGAAAACCACGGAGGCTTTGGGATTGAATGATAAGATGCAAGAAGATGATCAATTAAAAATAGGCAGACGAAATTTTTATTCAAAGGATATCAAGAATGCAAATGAAAACTACTTTGGTTCATATAGCTCTTTTGGCATACACAGAGAGATGTTAAGTGACAAGGTTCTCTACTAACTCCCTGTCACTTTTTAATGGAACATCATAAATTGATTTATTATAGAGGGGGATATTTCATTTTTGAAGTAATATATCACCGACATTACCATTTATGCAGGTCAGAATGGAAGCTTATATGCAAACTATTTTGCAAAACCCCTCTCTCTTCCACAATGCAGCTGTGATGGATGTAGGTTGTGGAACAGGCATACTTAGGTGAGCTGGAAAATAAATTCATACAATTGGCTGCAATTTTACATTTGGGCTTTTGCAATTGCCACTCGTTTCTATAATATTTCAACATTGGCATTATAATGACGTGCAATAACTACAAACAGTTCCCACAGGCTATTTTTATATATATTTTTAGTCCCGACCTGTCATGTTTTTCAATCAAGTTTGGTTGACCTTGACAAACCATATACTCGTCATTATTCATCTATTAGAAGGTTTATGTATTCTTCATATGTTGCTCTTCTCTTCTATAGTCTCTTTGCTGCAAAAGCGGGTGCGTCAAGAGTTATTGCAGTTGAAGCAAGTGAAAAGATGGCAGCAGTGGCCACCCAAGTAGTGAATCTCAACTTCAATATAGTCATTTAATTTCTCTGATAGTTTTTGTTGAGCTCCTTTACTGGACTTGCCTGCAGATTGCAAAAGACAATGGCCTTGGGCGGAATAGGAAACAGACTGGAGACTGCAGCATGTCAAGTGGGACTATAGAAGTGGTTCATGGTATGGTAGAAGAACTTGATAAGAACATAGAGATTCAGCCTCACAGTGTTGACGTATTATTGAGTGAATGGATGGGCTACTGCTTGCTGTATGAGTCAATGCTCAGCTCAGTTCTCTTTGCAAGGGATCGTTGGTTGAAGCCTGGAGGTGCAATCTTACCAGATACTGCCACAATTGTAAGTCTGGAGTATTTTGATTCACCTTGTGAGAGGTCTATCATGAAATTATATTTGTTTTTGTTATTAGTAATTTCTCTATCTACTTTTCAGCTTGTTGCAGGATTTGGAGTTGGTGGAACTAGTCTTCCATTTTGGGAAAATGTGTATGGTTTCAGCATGTCTTGTGTAGGGAAGGAACTTGTAGAGGATGCTGCCAAAGGTCCCATCGTTGACATTGTGGATGCTAAAGATCTAGTAACTAGTGCTGCAATTCTTCATGTAAGTACTACCCAAAAAAAACTTTTATTCATTGTGGACGCATTGCACCCTAGTACTACAGCTTGGTGAAAATGTTTTTCTCTATGGTTCTTAGAATCACGAGTGTTCTTCCTGCTGGGCCTGAAAGAATTATACTTGGAAAGGGATGCGATTACTGGGGCAAAAACATTTTAATGTGCTTATTAGATAGTTTGTGACTTCTTTTTTTTTCTATTCAGGAACACTATTTCTTGTATACTTCATCTTAGAAAGTCAGACTACTGTTTTAGTTATACTCACATCATCACGCATCTAGTGGGACTCATGGTCTTCGTTACTTGCACACCTCTTTTTTAGTGATTTTTCTCCCTCGCTGAATAGAGATAAAAATTGGATCCATTGTGCTGGGTTAAAAGATTTCATATATTTTTTGCCCCAATTAGTTCAATTACGTTTTCCTCTCTTCTACTAATGATTGAAACGGTTGCAAGTCTTTAGCTGTTTCTTCAATTACTAGACTAGCCTGCACCTCAATTATCCATGATTTTTATATCAGTTGAATGTCGTAGGTATGGTTTAACTTTAAGGATGATCTGAGTGCATTTCCTACATTCTTGATATATTGTGGATAACAGAATGGGTTGACTGGTTTAATCCCTACCGTGTATAATAACTGTTTTTTTTTTTTTTTTTGAAATCTTCTAATTTGGCTTGGGAGTTAATGAAGATATGCCTTCTTATTTTTTTTTTCAGACTTTTGATCTAGCAACTATGCATCCTGACGAAGTAGACTTCACTGCAAGTACTGAGTTGGAACCAAATTTACATTCTCCTTCTAGCAACTCAGAGAATGTTGAAGTTGAAACCTCTTGGTGTCATGGAGTTGTCCTATGGTTTGAAACTGGTTTTACAAGCAGATTTTGCAAAGAATCATCGGCTGTTCTGTCTACATCCCCGTACACTCCCAAAACTCACTGGTCTCAAACCATATTAACCTTCAGAGAACCAATTGCTATAACATCTAGAAAATCTGCCAGCAACAAATCAGCACCAGTAGGCAGCGATGCCTCCCCTGCTGTCAAAGTTCATTTGCGCCTTAGCATCGTCCGTGCGCCCGAACATCGAAGCATTGACATTTCACTGGAGACTACTGGGATTGCTGGTGATGGTAGGAAGCGCCACTGGCCTGTACAACTTTTCACACTAAGGTAGAGTACGCAACCGTAATTGGATCCTGAATCAGCAATTTCAGACTAAAAACTGGTCTGATTTTTAATTATGTTTTTGGAACTTGTTCTATCTCCATATGTGTTATTTGATAGTAGCAGAAGATCCATTCATGGAATTAATCAATTTTGGCTCTTAATTCTTGATATGTAAGTTATGATTAATAGGCATTGAGAATGTAAACGAGTTAAGCTAGTTAACTAGAGAGGCATATTGCTCTGTGTCTATATTATTGTGGTATTTGTTATTGAGGCAATTTTGTTTTGATTATGTATCCTATTGGTCTTGTATTTTCACAACATAATGAGAAA

mRNA sequence

ATGGATCAATTTCCCAAAGAACTCGACAAAGAATTCTGCAACACCGAGAAAATTGAAAGAGAAGAAGAAGAAGACGACGAGGACGACGAGCAAGAAGATGGACAAGACGACTGGGACGATTGGAATGCAGACGAGGAAGAAGGCGAAGAAGCATTTGACTCGAATTTTCTGTGTCTGTTCTGCAACTCCAAGTATAGTGACTGCGACACGCTGTTCAGACATTGCAACTCGGTCCACTACTTCGATTTTCATGACATTAGGAGGGCTCTGGGTTTAGACTTTTATGGTTCGTTTAAAATCATCAACTACATACGATCTCAGGTAGCAGAAAATAGGTGTTGGAGCTGTGGTCTCTTGTATCAATCAAACCAGGATTTACAGAACCATTTACACAAAACATTCAATCTTAGTGACATCAAGGCTCTTTGGGATGATGATAAATATCTAAAACCCTTCAGGCAAGATGATCCCCTCCTGTACAGTTTTGCAGAAGACGAAGAGGATGATGACATTTCACTCATGAACAATACGGATACTGATGGAGGTAATTGCAATGTTGATGAAATGACTGGAGAAAGTCTTCCATTTCATCCAAACCGACACAAAGAAAATGGAATGGAAGATTGTAGTTCTACTTCACACAGTTTTTCAAATGCTCCAAGAAACTTAGAGGCCGTGATTGAGAATACCGTGAAAACCACGGAGGCTTTGGGATTGAATGATAAGATGCAAGAAGATGATCAATTAAAAATAGGCAGACGAAATTTTTATTCAAAGGATATCAAGAATGCAAATGAAAACTACTTTGGTTCATATAGCTCTTTTGGCATACACAGAGAGATGTTAAGTGACAAGGTCAGAATGGAAGCTTATATGCAAACTATTTTGCAAAACCCCTCTCTCTTCCACAATGCAGCTGTGATGGATGTAGGTTGTGGAACAGGCATACTTAGTCTCTTTGCTGCAAAAGCGGGTGCGTCAAGAGTTATTGCAGTTGAAGCAAGTGAAAAGATGGCAGCAGTGGCCACCCAAATTGCAAAAGACAATGGCCTTGGGCGGAATAGGAAACAGACTGGAGACTGCAGCATGTCAAGTGGGACTATAGAAGTGGTTCATGGTATGGTAGAAGAACTTGATAAGAACATAGAGATTCAGCCTCACAGTGTTGACGTATTATTGAGTGAATGGATGGGCTACTGCTTGCTGTATGAGTCAATGCTCAGCTCAGTTCTCTTTGCAAGGGATCGTTGGTTGAAGCCTGGAGGTGCAATCTTACCAGATACTGCCACAATTCTTGTTGCAGGATTTGGAGTTGGTGGAACTAGTCTTCCATTTTGGGAAAATGTGTATGGTTTCAGCATGTCTTGTGTAGGGAAGGAACTTGTAGAGGATGCTGCCAAAGGTCCCATCGTTGACATTGTGGATGCTAAAGATCTAGTAACTAGTGCTGCAATTCTTCATACTTTTGATCTAGCAACTATGCATCCTGACGAAGTAGACTTCACTGCAAGTACTGAGTTGGAACCAAATTTACATTCTCCTTCTAGCAACTCAGAGAATGTTGAAGTTGAAACCTCTTGGTGTCATGGAGTTGTCCTATGGTTTGAAACTGGTTTTACAAGCAGATTTTGCAAAGAATCATCGGCTGTTCTGTCTACATCCCCGTACACTCCCAAAACTCACTGGTCTCAAACCATATTAACCTTCAGAGAACCAATTGCTATAACATCTAGAAAATCTGCCAGCAACAAATCAGCACCAGTAGGCAGCGATGCCTCCCCTGCTGTCAAAGTTCATTTGCGCCTTAGCATCGTCCGTGCGCCCGAACATCGAAGCATTGACATTTCACTGGAGACTACTGGGATTGCTGGTGATGGTAGGAAGCGCCACTGGCCTGTACAACTTTTCACACTAAGGTAG

Coding sequence (CDS)

ATGGATCAATTTCCCAAAGAACTCGACAAAGAATTCTGCAACACCGAGAAAATTGAAAGAGAAGAAGAAGAAGACGACGAGGACGACGAGCAAGAAGATGGACAAGACGACTGGGACGATTGGAATGCAGACGAGGAAGAAGGCGAAGAAGCATTTGACTCGAATTTTCTGTGTCTGTTCTGCAACTCCAAGTATAGTGACTGCGACACGCTGTTCAGACATTGCAACTCGGTCCACTACTTCGATTTTCATGACATTAGGAGGGCTCTGGGTTTAGACTTTTATGGTTCGTTTAAAATCATCAACTACATACGATCTCAGGTAGCAGAAAATAGGTGTTGGAGCTGTGGTCTCTTGTATCAATCAAACCAGGATTTACAGAACCATTTACACAAAACATTCAATCTTAGTGACATCAAGGCTCTTTGGGATGATGATAAATATCTAAAACCCTTCAGGCAAGATGATCCCCTCCTGTACAGTTTTGCAGAAGACGAAGAGGATGATGACATTTCACTCATGAACAATACGGATACTGATGGAGGTAATTGCAATGTTGATGAAATGACTGGAGAAAGTCTTCCATTTCATCCAAACCGACACAAAGAAAATGGAATGGAAGATTGTAGTTCTACTTCACACAGTTTTTCAAATGCTCCAAGAAACTTAGAGGCCGTGATTGAGAATACCGTGAAAACCACGGAGGCTTTGGGATTGAATGATAAGATGCAAGAAGATGATCAATTAAAAATAGGCAGACGAAATTTTTATTCAAAGGATATCAAGAATGCAAATGAAAACTACTTTGGTTCATATAGCTCTTTTGGCATACACAGAGAGATGTTAAGTGACAAGGTCAGAATGGAAGCTTATATGCAAACTATTTTGCAAAACCCCTCTCTCTTCCACAATGCAGCTGTGATGGATGTAGGTTGTGGAACAGGCATACTTAGTCTCTTTGCTGCAAAAGCGGGTGCGTCAAGAGTTATTGCAGTTGAAGCAAGTGAAAAGATGGCAGCAGTGGCCACCCAAATTGCAAAAGACAATGGCCTTGGGCGGAATAGGAAACAGACTGGAGACTGCAGCATGTCAAGTGGGACTATAGAAGTGGTTCATGGTATGGTAGAAGAACTTGATAAGAACATAGAGATTCAGCCTCACAGTGTTGACGTATTATTGAGTGAATGGATGGGCTACTGCTTGCTGTATGAGTCAATGCTCAGCTCAGTTCTCTTTGCAAGGGATCGTTGGTTGAAGCCTGGAGGTGCAATCTTACCAGATACTGCCACAATTCTTGTTGCAGGATTTGGAGTTGGTGGAACTAGTCTTCCATTTTGGGAAAATGTGTATGGTTTCAGCATGTCTTGTGTAGGGAAGGAACTTGTAGAGGATGCTGCCAAAGGTCCCATCGTTGACATTGTGGATGCTAAAGATCTAGTAACTAGTGCTGCAATTCTTCATACTTTTGATCTAGCAACTATGCATCCTGACGAAGTAGACTTCACTGCAAGTACTGAGTTGGAACCAAATTTACATTCTCCTTCTAGCAACTCAGAGAATGTTGAAGTTGAAACCTCTTGGTGTCATGGAGTTGTCCTATGGTTTGAAACTGGTTTTACAAGCAGATTTTGCAAAGAATCATCGGCTGTTCTGTCTACATCCCCGTACACTCCCAAAACTCACTGGTCTCAAACCATATTAACCTTCAGAGAACCAATTGCTATAACATCTAGAAAATCTGCCAGCAACAAATCAGCACCAGTAGGCAGCGATGCCTCCCCTGCTGTCAAAGTTCATTTGCGCCTTAGCATCGTCCGTGCGCCCGAACATCGAAGCATTGACATTTCACTGGAGACTACTGGGATTGCTGGTGATGGTAGGAAGCGCCACTGGCCTGTACAACTTTTCACACTAAGGTAG

Protein sequence

MDQFPKELDKEFCNTEKIEREEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCLFCNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLYQSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLMNNTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTEALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPAVKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR
Homology
BLAST of Spg009657 vs. NCBI nr
Match: XP_022966971.1 (probable protein arginine N-methyltransferase 3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 560/647 (86.55%), Postives = 586/647 (90.57%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIER---EEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFL 60
           MDQFPKELD+E CN  KIER   EEEE+DE++E+ED QDDWDDWNADEEEGEE FD NFL
Sbjct: 1   MDQFPKELDREVCNMNKIERKEEEEEEEDEEEEEEDVQDDWDDWNADEEEGEEGFDLNFL 60

Query: 61  CLFCNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCG 120
           CLFC SKY+DC TLF HCNSVH FDFH IR+ALGLDFYGSFKIINYIRSQVAENRCWSCG
Sbjct: 61  CLFCTSKYNDCATLFNHCNSVHNFDFHGIRKALGLDFYGSFKIINYIRSQVAENRCWSCG 120

Query: 121 LLYQSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDIS----- 180
            L QS+QDLQNHLH+T NL++IKALW+DDKYLKPF +DDP+LYSFAEDEEDDDIS     
Sbjct: 121 FLCQSSQDLQNHLHQTLNLNEIKALWNDDKYLKPFSEDDPILYSFAEDEEDDDISPITNN 180

Query: 181 LMNNTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVK 240
           L  NTD++ GNCNVD M     PFH N  KEN M DCSSTSH + N P NLEAVIENTVK
Sbjct: 181 LQRNTDSNCGNCNVDGMNRADPPFHINGSKENEMADCSSTSHGYLNTPSNLEAVIENTVK 240

Query: 241 TTEALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYM 300
            TE L LNDK QEDDQLKI RRNFYSKDIKN NE+YFGSYSSFGIHREMLSDKVRM+AYM
Sbjct: 241 ATETLKLNDKTQEDDQLKITRRNFYSKDIKNVNESYFGSYSSFGIHREMLSDKVRMDAYM 300

Query: 301 QTILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLG 360
           Q ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGL 
Sbjct: 301 QAILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLL 360

Query: 361 RNRKQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLF 420
           RNRKQTGDC MSSGT+EVV+GMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLF
Sbjct: 361 RNRKQTGDCGMSSGTVEVVNGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLF 420

Query: 421 ARDRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVD 480
           ARDRWLKPGGAILPDTATILVAGFG+GGTSLPFWENVYGF+MSCVGKELVEDAAKGPIVD
Sbjct: 421 ARDRWLKPGGAILPDTATILVAGFGIGGTSLPFWENVYGFNMSCVGKELVEDAAKGPIVD 480

Query: 481 IVDAKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVV 540
            V+A DLVTSAAILHTFDLATMH DEVDFTASTELEPNLH+PSS SENVEVETSWCHGVV
Sbjct: 481 TVEANDLVTSAAILHTFDLATMHADEVDFTASTELEPNLHNPSSKSENVEVETSWCHGVV 540

Query: 541 LWFETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDA 600
           LWFETGFTSRFCKES AVLSTSPYTPKTHWSQTILTFREPIAITS KS +NKSA VGS A
Sbjct: 541 LWFETGFTSRFCKESPAVLSTSPYTPKTHWSQTILTFREPIAITSSKSTTNKSATVGSFA 600

Query: 601 SPAVKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           SPAVKVHLRLSIVRAPEHRSIDISLETTGIA DGRK HWPVQLFTLR
Sbjct: 601 SPAVKVHLRLSIVRAPEHRSIDISLETTGIASDGRKHHWPVQLFTLR 647

BLAST of Spg009657 vs. NCBI nr
Match: KAG7012865.1 (putative protein arginine N-methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 560/654 (85.63%), Postives = 591/654 (90.37%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIER-EEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCL 60
           MDQFPKELD+E CN  KIER EEEE++E++E+ED QDDWDDWNADEEEGEE FDS+FLCL
Sbjct: 1   MDQFPKELDREVCNMNKIEREEEEEEEEEEEEEDVQDDWDDWNADEEEGEEGFDSSFLCL 60

Query: 61  FCNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLL 120
           FC SKY+DC TL  HCNSVH FDFH IR+ LGLDFYGSFKIINYIRSQVAENRCWSCG L
Sbjct: 61  FCTSKYNDCATLLNHCNSVHNFDFHGIRKCLGLDFYGSFKIINYIRSQVAENRCWSCGFL 120

Query: 121 YQSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDIS-----LM 180
            QS+QDLQNHLHKT NL++IKALW+DDKYLKPF +DDPLLYSFAEDEEDDDIS     L 
Sbjct: 121 CQSSQDLQNHLHKTLNLNEIKALWNDDKYLKPFSEDDPLLYSFAEDEEDDDISPITNNLQ 180

Query: 181 NNTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTT 240
            NTDT+GGNCNVDEM   SLPF+ NR KE+ M DCSS+SH + N P NLEAVIENTVK T
Sbjct: 181 RNTDTNGGNCNVDEMNRASLPFNLNRSKEHEMADCSSSSHGYLNTPINLEAVIENTVKAT 240

Query: 241 EALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQT 300
           E L LNDKMQEDD+LKI RRNF+SKDIKN NE+YFGSYSSFGIHREMLSDKVRM+AYMQ 
Sbjct: 241 ETLELNDKMQEDDRLKITRRNFFSKDIKNVNESYFGSYSSFGIHREMLSDKVRMDAYMQA 300

Query: 301 ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRN 360
           ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGL RN
Sbjct: 301 ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLLRN 360

Query: 361 RKQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR 420
           RKQ GDC MSSGT+EVV+GMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR
Sbjct: 361 RKQGGDCGMSSGTVEVVNGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR 420

Query: 421 DRWLKPGGAILPDTATI---------LVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDA 480
           DRWLKPGGAILPDTATI         LVAGFGVGGTSLPFWENVYGF+MSCVGKELVEDA
Sbjct: 421 DRWLKPGGAILPDTATIVISSLSTFQLVAGFGVGGTSLPFWENVYGFNMSCVGKELVEDA 480

Query: 481 AKGPIVDIVDAKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVET 540
           AKGPIVD V+A DLVTSAAILHTFDLATMH DEVDFTASTELEPNLH+PSS SEN E+ET
Sbjct: 481 AKGPIVDTVEANDLVTSAAILHTFDLATMHADEVDFTASTELEPNLHNPSSKSENGEIET 540

Query: 541 SWCHGVVLWFETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKS 600
           SWCHGVVLWFETGFTSRFCKES AVLSTSPYTPKTHWSQTILTFREPIAITS KSA+NKS
Sbjct: 541 SWCHGVVLWFETGFTSRFCKESPAVLSTSPYTPKTHWSQTILTFREPIAITSSKSATNKS 600

Query: 601 APVGSDASPAVKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           A VGS ASPA+KVHLRLSIVRAPEHRSIDISLETTGIAGDGRK HWPVQLFTLR
Sbjct: 601 ATVGSLASPAIKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKHHWPVQLFTLR 654

BLAST of Spg009657 vs. NCBI nr
Match: XP_022945828.1 (probable protein arginine N-methyltransferase 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 557/645 (86.36%), Postives = 588/645 (91.16%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIER-EEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCL 60
           MDQFPKELD+E CN  KIER EEEE++E++E+ED QDDWDDWNADEEEGEE FDS+FLCL
Sbjct: 1   MDQFPKELDREVCNMNKIEREEEEEEEEEEEEEDVQDDWDDWNADEEEGEEGFDSSFLCL 60

Query: 61  FCNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLL 120
           FC SKY+DC TL  HCNSVH FDFH IR+ LGLDFYGSFKIINYIRSQVAENRCWSCG L
Sbjct: 61  FCTSKYNDCATLLNHCNSVHNFDFHGIRKGLGLDFYGSFKIINYIRSQVAENRCWSCGFL 120

Query: 121 YQSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDIS-----LM 180
            QS+QDLQNHLHKT NL++IKALW+DDKYLKPF +DDPLLYSFAEDEEDDDIS     L 
Sbjct: 121 CQSSQDLQNHLHKTLNLNEIKALWNDDKYLKPFSEDDPLLYSFAEDEEDDDISPITNNLQ 180

Query: 181 NNTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTT 240
            NTDT+GGNCNVDEM   SLPF+ NR KE+ M DCSS+SH + N P NLEAVIENTVK T
Sbjct: 181 RNTDTNGGNCNVDEMNRASLPFNLNRSKEHEMADCSSSSHGYLNTPINLEAVIENTVKAT 240

Query: 241 EALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQT 300
           E L LNDKMQEDD+LKI  RNF+SKDIKN NE+YFGSYSSFGIHREMLSDKVRM+AYMQ 
Sbjct: 241 ETLELNDKMQEDDRLKITGRNFFSKDIKNVNESYFGSYSSFGIHREMLSDKVRMDAYMQA 300

Query: 301 ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRN 360
           ILQNPSLF NAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGL RN
Sbjct: 301 ILQNPSLFRNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLLRN 360

Query: 361 RKQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR 420
           RKQ GDC MSSGT+EVV+GMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR
Sbjct: 361 RKQGGDCGMSSGTVEVVNGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR 420

Query: 421 DRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIV 480
           DRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGF+MSCVGKELVEDAAKGPIVD V
Sbjct: 421 DRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFNMSCVGKELVEDAAKGPIVDTV 480

Query: 481 DAKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLW 540
           +A DLVTSAAILHTFDLATMH DEVDFTASTELEPNLH PSS SEN E+ETSWCHGVVLW
Sbjct: 481 EANDLVTSAAILHTFDLATMHADEVDFTASTELEPNLHKPSSKSENGEIETSWCHGVVLW 540

Query: 541 FETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASP 600
           FETGFTSRFCKES AVLSTSPYTPKTHWSQTILTFREPIAITS KSA+NKSA VGS ASP
Sbjct: 541 FETGFTSRFCKESPAVLSTSPYTPKTHWSQTILTFREPIAITSSKSATNKSATVGSLASP 600

Query: 601 AVKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           A+KVHLRLSIVRAP+HRSIDISLETTGIAGDGRK HWPVQLFTLR
Sbjct: 601 AIKVHLRLSIVRAPKHRSIDISLETTGIAGDGRKHHWPVQLFTLR 645

BLAST of Spg009657 vs. NCBI nr
Match: XP_022991200.1 (probable protein arginine N-methyltransferase 3 [Cucurbita maxima])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 551/644 (85.56%), Postives = 584/644 (90.68%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIEREEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCLF 60
           M++FP +LD+E C TEK EREE++DD+DDEQEDG+DDWDDWNADEE  EEAFDSNFLCLF
Sbjct: 1   MNRFPNDLDEEICITEKFEREEDDDDDDDEQEDGEDDWDDWNADEEGSEEAFDSNFLCLF 60

Query: 61  CNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLY 120
           CNSKY+DCDTLF HCNSVHYFDFH IRR LGLDFYGSFK+INYIRSQVAENRCWSCG L 
Sbjct: 61  CNSKYNDCDTLFNHCNSVHYFDFHGIRRTLGLDFYGSFKVINYIRSQVAENRCWSCGFLC 120

Query: 121 QSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLMN----- 180
           QSNQDLQNHLHKT NLSD+KA WDDDKYL PFRQDDPLLYSFAEDEEDD+ISL+N     
Sbjct: 121 QSNQDLQNHLHKTLNLSDVKAFWDDDKYLNPFRQDDPLLYSFAEDEEDDEISLINNDLQR 180

Query: 181 NTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTE 240
           N  TDGGNCN DEM+ E+LPFHPN++KENGMEDC S+ H + NAP NLEA IENTVKTTE
Sbjct: 181 NAGTDGGNCNHDEMSRENLPFHPNQNKENGMEDCCSSLHGYLNAPSNLEAEIENTVKTTE 240

Query: 241 ALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTI 300
            +GL     +D+QLKI RRN    DIKNAN NYF SYSSFGIHREMLSDKVR EAYM  I
Sbjct: 241 IVGL-----KDNQLKIARRNVDRMDIKNANVNYFDSYSSFGIHREMLSDKVRTEAYMHAI 300

Query: 301 LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNR 360
           LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRV+AVEASEKMAAVATQIAKDNGL RN 
Sbjct: 301 LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVLAVEASEKMAAVATQIAKDNGLWRNE 360

Query: 361 KQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARD 420
           KQTGD  +SSGTIEVVHGMVEELDKNI+IQPHSVDVL+SEWMGYCLLYESML+SVLFARD
Sbjct: 361 KQTGDYGVSSGTIEVVHGMVEELDKNIKIQPHSVDVLVSEWMGYCLLYESMLNSVLFARD 420

Query: 421 RWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVD 480
           RWLKPGGAILPDTATILVAGFG GGTSLPFWENVYGFSMSCVGKELV+DAAK PIVDIVD
Sbjct: 421 RWLKPGGAILPDTATILVAGFGAGGTSLPFWENVYGFSMSCVGKELVKDAAKAPIVDIVD 480

Query: 481 AKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWF 540
           A DLVTS AILHTFDLATMHPDEVDFTAST+LEPNLH PSSNS++VEVE SWCHGVVLWF
Sbjct: 481 ANDLVTSTAILHTFDLATMHPDEVDFTASTDLEPNLHYPSSNSDDVEVEASWCHGVVLWF 540

Query: 541 ETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPA 600
           ETGFTSRFCKES AVLSTSP+TPKTHWSQTILTF EPIAITSRKSA+NKSAPVGS ASPA
Sbjct: 541 ETGFTSRFCKESPAVLSTSPHTPKTHWSQTILTFTEPIAITSRKSATNKSAPVGSHASPA 600

Query: 601 VKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           VKVHLRLSIVRAPEHRSIDISLETTGIAGDGR R WPVQLFTLR
Sbjct: 601 VKVHLRLSIVRAPEHRSIDISLETTGIAGDGRNRDWPVQLFTLR 639

BLAST of Spg009657 vs. NCBI nr
Match: XP_023542552.1 (probable protein arginine N-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 556/645 (86.20%), Postives = 584/645 (90.54%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIER-EEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCL 60
           MDQFPKE D+E  N  KIER EEEE+D+++E+ED QDDWDDWNADEEEGEE FDS+FLCL
Sbjct: 1   MDQFPKEFDREVWNMNKIEREEEEEEDDEEEEEDVQDDWDDWNADEEEGEEGFDSSFLCL 60

Query: 61  FCNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLL 120
           FC SKY+DC TLF HCNSVH FDFH IR+ LGLDFYGSFKIINYIRSQVAENRCWSCG L
Sbjct: 61  FCTSKYNDCATLFNHCNSVHNFDFHGIRKGLGLDFYGSFKIINYIRSQVAENRCWSCGFL 120

Query: 121 YQSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLM----- 180
            QS+QDLQNHLHKT NL++IKALW+DDKYLKPF +DDPLLYSFAE EEDDDIS +     
Sbjct: 121 CQSSQDLQNHLHKTLNLNEIKALWNDDKYLKPFSEDDPLLYSFAEAEEDDDISPITNNHQ 180

Query: 181 NNTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTT 240
            NTDT+GGNCN DE+   SLPFH NR  EN M DCSSTSH + N P NLE VIENTVK T
Sbjct: 181 RNTDTNGGNCNTDEINRASLPFHLNRSNENEMADCSSTSHGYLNTPSNLEVVIENTVKAT 240

Query: 241 EALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQT 300
           E L LNDK QEDD+LKI RRNFYSKDIKN NE+YFGSYSSFGIHREMLSDKVRM+AYMQ 
Sbjct: 241 ETLELNDKTQEDDRLKITRRNFYSKDIKNVNESYFGSYSSFGIHREMLSDKVRMDAYMQA 300

Query: 301 ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRN 360
           ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGL RN
Sbjct: 301 ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLLRN 360

Query: 361 RKQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR 420
           RKQTGDC MSSGT+EVV+GMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR
Sbjct: 361 RKQTGDCGMSSGTVEVVNGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR 420

Query: 421 DRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIV 480
           DRWLKPGGAILPDTATILVAGFGVGGTSLPFWE+VYGF+MSCVGKELVEDAAKGPIVD V
Sbjct: 421 DRWLKPGGAILPDTATILVAGFGVGGTSLPFWEDVYGFNMSCVGKELVEDAAKGPIVDTV 480

Query: 481 DAKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLW 540
           +A DLVTSAAILHTFDLATMH DEVDFTASTELEPNLH+PSS SEN E ETSWCHGVVLW
Sbjct: 481 EANDLVTSAAILHTFDLATMHADEVDFTASTELEPNLHNPSSKSENGETETSWCHGVVLW 540

Query: 541 FETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASP 600
           FETGFTSRFCKES AVLSTSPYTPKTHWSQTILTFREPIAITS KSA+NKSA VGS ASP
Sbjct: 541 FETGFTSRFCKESPAVLSTSPYTPKTHWSQTILTFREPIAITSSKSATNKSAAVGSFASP 600

Query: 601 AVKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           AVKVHLRLSIVRAPEHRSIDISLETTGIA DGRK HWPVQLFTLR
Sbjct: 601 AVKVHLRLSIVRAPEHRSIDISLETTGIASDGRKHHWPVQLFTLR 645

BLAST of Spg009657 vs. ExPASy Swiss-Prot
Match: Q0WVD6 (Probable protein arginine N-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=PRMT3 PE=2 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 2.6e-179
Identity = 345/632 (54.59%), Postives = 437/632 (69.15%), Query Frame = 0

Query: 24  EDDEDDEQEDGQDDWDDWNAD-------EEEGEEAFD---SNFLCLFCNSKYSDCDTLFR 83
           ED+E++  ++G  DW DW AD       EEE E+  D   S+FLCLFC+S +  CD LF 
Sbjct: 15  EDEEENYSDEG--DWGDWKADDNGIEGGEEEEEDDGDDSESDFLCLFCDSHFVSCDLLFE 74

Query: 84  HCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLYQSNQDLQNHLHKT 143
           HC   H FDFH +R+ L LDFY SFK+INYIRSQVAEN C+S  +             + 
Sbjct: 75  HCRLSHGFDFHGVRKELKLDFYSSFKLINYIRSQVAENMCFSWKI-------------EA 134

Query: 144 FNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLMNNTDTDG----GNCNVD-E 203
            +  D+K  WD++KYLKPF Q+D LLYSFA+DEED++++       +     G+ ++D E
Sbjct: 135 DDYKDVKFPWDEEKYLKPFWQEDSLLYSFADDEEDEEVTFDREEVMEELQKLGDLSIDVE 194

Query: 204 MTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTEALGLNDKMQEDDQ 263
             GES   + ++   NG +D +S S+       + + +I N               +D +
Sbjct: 195 ALGESSMSNSDKCNINGSKDVTSLSNCNGLKQSSADDLIVN--------------GKDAE 254

Query: 264 LKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVM 323
            K+      +++I+  NENYFGSYSSFGIHREMLSDKVR EAY   +L+NP+L + + VM
Sbjct: 255 PKVCDGRLVNRNIRKVNENYFGSYSSFGIHREMLSDKVRTEAYRDALLKNPTLLNGSVVM 314

Query: 324 DVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTI 383
           DVGCGTGILSLFAAKAGASRV+AVEASEKMA VAT+IAKDN +  + +        +G +
Sbjct: 315 DVGCGTGILSLFAAKAGASRVVAVEASEKMAKVATKIAKDNKVFNDNEH-------NGVL 374

Query: 384 EVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDT 443
           EV H MVEELDK+I+IQPHSVDVL+SEWMGYCLLYESMLSSVL+ARDRWLKPGGAILPDT
Sbjct: 375 EVAHSMVEELDKSIQIQPHSVDVLVSEWMGYCLLYESMLSSVLYARDRWLKPGGAILPDT 434

Query: 444 ATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHT 503
           AT+ VAGFG G TSLPFWE+VYGF MS +GKE+ +D  + PIVD++  +DLVT   +L T
Sbjct: 435 ATMFVAGFGKGATSLPFWEDVYGFDMSSIGKEIHDDTTRLPIVDVIAERDLVTQPTLLQT 494

Query: 504 FDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESS 563
           FDLATM PDEVDFTA+  LEP            E +T  CHGVVLWF+TGFTSRFCKE+ 
Sbjct: 495 FDLATMKPDEVDFTATATLEPT---------ESEAKTRLCHGVVLWFDTGFTSRFCKENP 554

Query: 564 AVLSTSPYTPKTHWSQTILTFREPIAI--TSRKSASNKSAPVGSDASPAVKVHLRLSIVR 623
            VLSTSPYTP THW+QTILTF+EPI++   S  S +++   +G++  PA  +HLR+S+ R
Sbjct: 555 TVLSTSPYTPPTHWAQTILTFQEPISVAPASVLSGNDRREAIGTEECPASSIHLRVSVAR 601

Query: 624 APEHRSIDISLETTGIAGDGRKRHWPVQLFTL 639
           A EHRSIDISLE TG++  G+KRHWPVQ+F L
Sbjct: 615 AHEHRSIDISLEATGLSSKGQKRHWPVQIFNL 601

BLAST of Spg009657 vs. ExPASy Swiss-Prot
Match: A3BMN9 (Probable protein arginine N-methyltransferase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PRMT3 PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 3.9e-167
Identity = 324/625 (51.84%), Postives = 419/625 (67.04%), Query Frame = 0

Query: 19  EREEEEDDEDDEQEDGQDDWDDWNAD-EEEGEEAFDSNFLCLFCNSKYSDCDTLFRHCNS 78
           E  EE +D ++E+E+G++ WDDW +D ++ G        LCLFC++++    +LF HC S
Sbjct: 17  EDREEYEDGEEEEEEGEEGWDDWESDGDDAGGGGGGGGLLCLFCSARFDSESSLFSHCAS 76

Query: 79  VHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLYQSNQDLQNHLHK-TFNL 138
            H FDF+ + +  G+DFYG  K+IN++RS+VAEN+CWSCG ++ SN +L  HLH      
Sbjct: 77  EHRFDFYRVVKETGMDFYGCIKLINFVRSKVAENKCWSCGQVFSSNSELCGHLHALEIPQ 136

Query: 139 SDIKALWDDDKYLKPFRQDDPLLYS---FAEDEEDDDISLMNNTDTDGGNCNVDEMTGES 198
            + K  W DD YLKPF +DD LL+S   F +D+EDD    M       GN ++ E    +
Sbjct: 137 LEGKVPWGDDVYLKPFLEDDSLLHSLSVFDDDDEDDCGMPMEKGGCSAGNGSLAETCESN 196

Query: 199 LPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTEALGLNDKMQEDDQLKIGR 258
           L        ++ + D S     F          IE+T    E  G   +   D QLKI R
Sbjct: 197 L--------KSIINDGSDVIDRFERT-----CTIEST--DGECSGSLAQEPSDKQLKIAR 256

Query: 259 RNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVMDVGCG 318
            +  ++ IK+ +E+YFGSYSSFGIHREML DKVR EAY   +L NPSL + A V+DVGCG
Sbjct: 257 ASAAARGIKSVDESYFGSYSSFGIHREMLGDKVRTEAYRDALLGNPSLMNGATVLDVGCG 316

Query: 319 TGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTIEVVHG 378
           TGILSLFAAKAGASRVIAV+ S KM +VAT++AK NG   +          +  I VVH 
Sbjct: 317 TGILSLFAAKAGASRVIAVDGSAKMVSVATEVAKSNGFLYDENMEMQQKRDTQVITVVHT 376

Query: 379 MVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDTATILV 438
             EEL+  I++  +  DVL+SEWMGYCLLYESMLSSVL+ARD +LKPGGAILPDTATI  
Sbjct: 377 KAEELNHKIQVPSNKFDVLVSEWMGYCLLYESMLSSVLYARDHFLKPGGAILPDTATIFG 436

Query: 439 AGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHTFDLAT 498
           AGFG GGTSLPFWENVYGF MSC+GKE+  ++A+ P+VDI+ ++D+VT  A+L++FDLAT
Sbjct: 437 AGFGKGGTSLPFWENVYGFDMSCIGKEVTGNSARFPVVDILASEDIVTETAVLNSFDLAT 496

Query: 499 MHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESSAVLST 558
           M  +E+DFT+S EL        S S   +   +WC+G++LWF+TGFT+RFCKE    LST
Sbjct: 497 MKENEMDFTSSFELR------LSESGVSQSGVTWCYGIILWFDTGFTNRFCKEKPVNLST 556

Query: 559 SPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPAVKVHLRLSIVRAPEHRSI 618
           SP++  THWSQTI TF EPIA+   +SA   SA VG+D  PAV +  R+SIVRA EHRSI
Sbjct: 557 SPFSTPTHWSQTIFTFEEPIAMAKEESAVVSSASVGTDECPAVMIRSRISIVRASEHRSI 616

Query: 619 DISLETTGIAGDGRKRHWPVQLFTL 639
           DIS+ETTGI+ DGRKR WPVQ+F L
Sbjct: 617 DISIETTGISSDGRKRSWPVQIFNL 620

BLAST of Spg009657 vs. ExPASy Swiss-Prot
Match: A2YP56 (Probable protein arginine N-methyltransferase 3 OS=Oryza sativa subsp. indica OX=39946 GN=PRMT3 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 6.2e-165
Identity = 321/625 (51.36%), Postives = 418/625 (66.88%), Query Frame = 0

Query: 19  EREEEED-DEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCLFCNSKYSDCDTLFRHCNS 78
           +REE ED +E++E+E+ ++ WDDW +D ++         LCLFC++++    +LF HC S
Sbjct: 18  DREEYEDGEEEEEEEEEEEGWDDWESDGDDAGGG-GGGLLCLFCSARFDSESSLFSHCAS 77

Query: 79  VHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLYQSNQDLQNHLHK-TFNL 138
            H FDF+ + +  G+DFYG  K+IN++RS+VAEN+CWSCG ++ SN +L  HLH      
Sbjct: 78  EHRFDFYRVVKETGMDFYGCIKLINFVRSKVAENKCWSCGQVFSSNSELCGHLHALEIPQ 137

Query: 139 SDIKALWDDDKYLKPFRQDDPLLYS---FAEDEEDDDISLMNNTDTDGGNCNVDEMTGES 198
            + K  W DD YLKPF +DD LL+S   F +D+EDD    M       GN ++ E    +
Sbjct: 138 LEGKVPWGDDVYLKPFLEDDSLLHSLSVFDDDDEDDCGMPMEKGGCSAGNGSLAETCESN 197

Query: 199 LPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTEALGLNDKMQEDDQLKIGR 258
           L        ++ + D S     F          IE+T    E  G   +   D QLKI R
Sbjct: 198 L--------KSIINDGSDVIDRFERT-----CTIEST--DGECSGSLAQEPSDKQLKIAR 257

Query: 259 RNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVMDVGCG 318
            +  ++ I + +E+YFGSYSSFGIHREML DKVR EAY   +L NPSL + A V+DVGCG
Sbjct: 258 ASAAARGINSVDESYFGSYSSFGIHREMLGDKVRTEAYRDALLGNPSLMNGATVLDVGCG 317

Query: 319 TGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTIEVVHG 378
           TGILSLFAAKAGASRVIAV+ S KM +VAT++ K NG   +          +  I VVH 
Sbjct: 318 TGILSLFAAKAGASRVIAVDGSAKMVSVATEVTKSNGFLYDENMEMQQKRDTQVITVVHT 377

Query: 379 MVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDTATILV 438
             EEL+  I++  +  DVL+SEWMGYCLLYESMLSSVL+ARD +LKPGGAILPDTATI  
Sbjct: 378 KAEELNHKIQVPSNKFDVLVSEWMGYCLLYESMLSSVLYARDHFLKPGGAILPDTATIFG 437

Query: 439 AGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHTFDLAT 498
           AGFG GGTSLPFWENVYGF MSC+GKE+  ++A+ P+VDI+ ++D+VT  A+L++FDLAT
Sbjct: 438 AGFGKGGTSLPFWENVYGFDMSCIGKEVTGNSARFPVVDILASEDIVTETAVLNSFDLAT 497

Query: 499 MHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESSAVLST 558
           M  +E+DFT+S EL  +    S +        +WC+G++LWF+TGFT+RFCKE    LST
Sbjct: 498 MKENEMDFTSSFELRLSESGVSPSG------VTWCYGIILWFDTGFTNRFCKEKPVNLST 557

Query: 559 SPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPAVKVHLRLSIVRAPEHRSI 618
           SP++  THWSQTI TF EPIA+   +SA   SA VG+D  PAV +  R+SIVRA EHRSI
Sbjct: 558 SPFSTPTHWSQTIFTFEEPIAMAKEESAVVSSASVGTDECPAVMIRSRISIVRASEHRSI 617

Query: 619 DISLETTGIAGDGRKRHWPVQLFTL 639
           DIS+ETTGI+ DGRKR WPVQ+F L
Sbjct: 618 DISIETTGISSDGRKRSWPVQIFNL 620

BLAST of Spg009657 vs. ExPASy Swiss-Prot
Match: O70467 (Protein arginine N-methyltransferase 3 OS=Rattus norvegicus OX=10116 GN=Prmt3 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 9.6e-57
Identity = 181/566 (31.98%), Postives = 266/566 (47.00%), Query Frame = 0

Query: 30  EQEDGQDD--WDDWNADEEEGEEAFDSNFLCLFCNSKYSDCDTLFRHCNSVHYFDFHDIR 89
           E  D  DD  W+D +AD E  +     +  CLFC+  +   +  F HC   H F+   + 
Sbjct: 20  ELSDSGDDAGWEDEDADAEPAQGR--QHTPCLFCDRLFRSAEETFSHCKLEHQFNIDGMV 79

Query: 90  RALGLDFYGSFKIINYIRSQVAENRCWSCGLLYQSNQDLQNHLHKTFNLSDIKALWDDDK 149
              GL+FYG  K+IN+IR                    L+N   +  N       WD D+
Sbjct: 80  HKHGLEFYGYIKLINFIR--------------------LKNPTVEYMNSIYNPVPWDKDE 139

Query: 150 YLKPFRQDDPLLYSFAEDEEDDDISLMNNTDTDGGNCNVDEMTGESLPF-HPNRHKENGM 209
           YLKP  +DD LL    ED  +                        S PF +P     NG+
Sbjct: 140 YLKPVLEDDLLLQFDVEDLYEP----------------------VSAPFTYP-----NGL 199

Query: 210 EDCSSTSHSFSNAPRNLEAVIENTVKTTEALGLNDKMQEDDQLKIGRRNFYS----KDIK 269
            + +S             +      +  E L    +  +D  + +  R   S     D++
Sbjct: 200 SENTSAVEKLKLMEARALSAEAALARAREDLQKMKQFAQDFVMNVDVRTCSSTTTIADLQ 259

Query: 270 NANEN-YFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVMDVGCGTGILSLFA 329
              +  YF SY  +GIH EML DKVR E+Y   I QNP +F +  V+DVGCGTGILS+FA
Sbjct: 260 EDEDGVYFSSYGHYGIHEEMLKDKVRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFA 319

Query: 330 AKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTIEVVHGMVEELDKN 389
           AKAGA +VIAV+ SE +   A  I + N L               TI ++ G +EE+   
Sbjct: 320 AKAGAKKVIAVDQSE-ILYQAMDIIRLNKL-------------EDTIVLIKGKIEEVSLP 379

Query: 390 IEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDTATI-LVAGFGVG- 449
           +E     VDV++SEWMGY LL+ESML SVL+A+ ++L  GG++ PD  TI LVA   V  
Sbjct: 380 VE----KVDVIISEWMGYFLLFESMLDSVLYAKSKYLAKGGSVYPDICTISLVAVSDVSK 439

Query: 450 -GTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHTFDLATMHPDE 509
               + FW++VYGF+MSC+ K ++ +A    +V++VD K L++    +   D  T    +
Sbjct: 440 HADRIAFWDDVYGFNMSCMKKAVIPEA----VVEVVDHKTLISDPCDIKHIDCHTTSISD 499

Query: 510 VDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESSAVLSTSPYTP 569
           ++F++   L                +T+ C  V  +F+  F  + C  +  V ST P + 
Sbjct: 500 LEFSSDFTLR-------------TTKTAMCTAVAGYFDIYF-EKNC-HNRVVFSTGPQST 499

Query: 570 KTHWSQTILTFREPIAITSRKSASNK 585
           KTHW QTI    +P  + + ++   K
Sbjct: 560 KTHWKQTIFLLEKPFPVKAGEALKGK 499

BLAST of Spg009657 vs. ExPASy Swiss-Prot
Match: O60678 (Protein arginine N-methyltransferase 3 OS=Homo sapiens OX=9606 GN=PRMT3 PE=1 SV=4)

HSP 1 Score: 210.7 bits (535), Expect = 4.9e-53
Identity = 178/572 (31.12%), Postives = 269/572 (47.03%), Query Frame = 0

Query: 21  EEEEDDEDDEQEDGQDDWDDW-NADEEEGEEAFDSNFLCLFCNSKYSDCDTLFRHCNSVH 80
           E EED  +      +  W+D  +AD   G++       CLFCN  ++  +  F HC S H
Sbjct: 16  ENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTP----CLFCNRLFTSAEETFSHCKSEH 75

Query: 81  YFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLYQSNQDLQNHLHKTFNLSDI 140
            F+   +    GL+FYG  K+IN+IR                    L+N   +  N    
Sbjct: 76  QFNIDSMVHKHGLEFYGYIKLINFIR--------------------LKNPTVEYMNSIYN 135

Query: 141 KALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLMNNTDTDGGNCNVDEMTGESLPF-HP 200
              W+ ++YLKP  +DD LL    ED  +                        S+PF +P
Sbjct: 136 PVPWEKEEYLKPVLEDDLLLQFDVEDLYEP----------------------VSVPFSYP 195

Query: 201 NRHKENG--MEDCSSTSHSFSNAPRNLEAVIENTVKTTEALGLNDKMQEDDQLKIGRRNF 260
           N   EN   +E          +A   L    E+  K  +     D +   D         
Sbjct: 196 NGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQF--AQDFVMHTDVRTCSSSTS 255

Query: 261 YSKDIKNANEN-YFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVMDVGCGTG 320
              D++   +  YF SY  +GIH EML DK+R E+Y   I QNP +F +  V+DVGCGTG
Sbjct: 256 VIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTG 315

Query: 321 ILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTIEVVHGMV 380
           ILS+FAAKAGA +V+ V+ SE +   A  I + N L               TI ++ G +
Sbjct: 316 ILSMFAAKAGAKKVLGVDQSE-ILYQAMDIIRLNKL-------------EDTITLIKGKI 375

Query: 381 EELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDTATI-LVA 440
           EE+   +E     VDV++SEWMGY LL+ESML SVL+A++++L  GG++ PD  TI LVA
Sbjct: 376 EEVHLPVE----KVDVIISEWMGYFLLFESMLDSVLYAKNKYLAKGGSVYPDICTISLVA 435

Query: 441 GFGVG--GTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHTFDLA 500
              V      + FW++VYGF MSC+ K ++ +A    +V+++D K L++    +   D  
Sbjct: 436 VSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEA----VVEVLDPKTLISEPCGIKHIDCH 495

Query: 501 TMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESSAVLS 560
           T    +++F++   L+                TS C  +  +F+  F  + C  +  V S
Sbjct: 496 TTSISDLEFSSDFTLK-------------ITRTSMCTAIAGYFDIYF-EKNC-HNRVVFS 502

Query: 561 TSPYTPKTHWSQTILTFREPIAITSRKSASNK 585
           T P + KTHW QT+    +P ++ + ++   K
Sbjct: 556 TGPQSTKTHWKQTVFLLEKPFSVKAGEALKGK 502

BLAST of Spg009657 vs. ExPASy TrEMBL
Match: A0A6J1HQS3 (probable protein arginine N-methyltransferase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466522 PE=4 SV=1)

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 560/647 (86.55%), Postives = 586/647 (90.57%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIER---EEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFL 60
           MDQFPKELD+E CN  KIER   EEEE+DE++E+ED QDDWDDWNADEEEGEE FD NFL
Sbjct: 1   MDQFPKELDREVCNMNKIERKEEEEEEEDEEEEEEDVQDDWDDWNADEEEGEEGFDLNFL 60

Query: 61  CLFCNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCG 120
           CLFC SKY+DC TLF HCNSVH FDFH IR+ALGLDFYGSFKIINYIRSQVAENRCWSCG
Sbjct: 61  CLFCTSKYNDCATLFNHCNSVHNFDFHGIRKALGLDFYGSFKIINYIRSQVAENRCWSCG 120

Query: 121 LLYQSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDIS----- 180
            L QS+QDLQNHLH+T NL++IKALW+DDKYLKPF +DDP+LYSFAEDEEDDDIS     
Sbjct: 121 FLCQSSQDLQNHLHQTLNLNEIKALWNDDKYLKPFSEDDPILYSFAEDEEDDDISPITNN 180

Query: 181 LMNNTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVK 240
           L  NTD++ GNCNVD M     PFH N  KEN M DCSSTSH + N P NLEAVIENTVK
Sbjct: 181 LQRNTDSNCGNCNVDGMNRADPPFHINGSKENEMADCSSTSHGYLNTPSNLEAVIENTVK 240

Query: 241 TTEALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYM 300
            TE L LNDK QEDDQLKI RRNFYSKDIKN NE+YFGSYSSFGIHREMLSDKVRM+AYM
Sbjct: 241 ATETLKLNDKTQEDDQLKITRRNFYSKDIKNVNESYFGSYSSFGIHREMLSDKVRMDAYM 300

Query: 301 QTILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLG 360
           Q ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGL 
Sbjct: 301 QAILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLL 360

Query: 361 RNRKQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLF 420
           RNRKQTGDC MSSGT+EVV+GMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLF
Sbjct: 361 RNRKQTGDCGMSSGTVEVVNGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLF 420

Query: 421 ARDRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVD 480
           ARDRWLKPGGAILPDTATILVAGFG+GGTSLPFWENVYGF+MSCVGKELVEDAAKGPIVD
Sbjct: 421 ARDRWLKPGGAILPDTATILVAGFGIGGTSLPFWENVYGFNMSCVGKELVEDAAKGPIVD 480

Query: 481 IVDAKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVV 540
            V+A DLVTSAAILHTFDLATMH DEVDFTASTELEPNLH+PSS SENVEVETSWCHGVV
Sbjct: 481 TVEANDLVTSAAILHTFDLATMHADEVDFTASTELEPNLHNPSSKSENVEVETSWCHGVV 540

Query: 541 LWFETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDA 600
           LWFETGFTSRFCKES AVLSTSPYTPKTHWSQTILTFREPIAITS KS +NKSA VGS A
Sbjct: 541 LWFETGFTSRFCKESPAVLSTSPYTPKTHWSQTILTFREPIAITSSKSTTNKSATVGSFA 600

Query: 601 SPAVKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           SPAVKVHLRLSIVRAPEHRSIDISLETTGIA DGRK HWPVQLFTLR
Sbjct: 601 SPAVKVHLRLSIVRAPEHRSIDISLETTGIASDGRKHHWPVQLFTLR 647

BLAST of Spg009657 vs. ExPASy TrEMBL
Match: A0A6J1G232 (probable protein arginine N-methyltransferase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449957 PE=4 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 557/645 (86.36%), Postives = 588/645 (91.16%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIER-EEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCL 60
           MDQFPKELD+E CN  KIER EEEE++E++E+ED QDDWDDWNADEEEGEE FDS+FLCL
Sbjct: 1   MDQFPKELDREVCNMNKIEREEEEEEEEEEEEEDVQDDWDDWNADEEEGEEGFDSSFLCL 60

Query: 61  FCNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLL 120
           FC SKY+DC TL  HCNSVH FDFH IR+ LGLDFYGSFKIINYIRSQVAENRCWSCG L
Sbjct: 61  FCTSKYNDCATLLNHCNSVHNFDFHGIRKGLGLDFYGSFKIINYIRSQVAENRCWSCGFL 120

Query: 121 YQSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDIS-----LM 180
            QS+QDLQNHLHKT NL++IKALW+DDKYLKPF +DDPLLYSFAEDEEDDDIS     L 
Sbjct: 121 CQSSQDLQNHLHKTLNLNEIKALWNDDKYLKPFSEDDPLLYSFAEDEEDDDISPITNNLQ 180

Query: 181 NNTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTT 240
            NTDT+GGNCNVDEM   SLPF+ NR KE+ M DCSS+SH + N P NLEAVIENTVK T
Sbjct: 181 RNTDTNGGNCNVDEMNRASLPFNLNRSKEHEMADCSSSSHGYLNTPINLEAVIENTVKAT 240

Query: 241 EALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQT 300
           E L LNDKMQEDD+LKI  RNF+SKDIKN NE+YFGSYSSFGIHREMLSDKVRM+AYMQ 
Sbjct: 241 ETLELNDKMQEDDRLKITGRNFFSKDIKNVNESYFGSYSSFGIHREMLSDKVRMDAYMQA 300

Query: 301 ILQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRN 360
           ILQNPSLF NAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGL RN
Sbjct: 301 ILQNPSLFRNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLLRN 360

Query: 361 RKQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR 420
           RKQ GDC MSSGT+EVV+GMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR
Sbjct: 361 RKQGGDCGMSSGTVEVVNGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFAR 420

Query: 421 DRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIV 480
           DRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGF+MSCVGKELVEDAAKGPIVD V
Sbjct: 421 DRWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFNMSCVGKELVEDAAKGPIVDTV 480

Query: 481 DAKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLW 540
           +A DLVTSAAILHTFDLATMH DEVDFTASTELEPNLH PSS SEN E+ETSWCHGVVLW
Sbjct: 481 EANDLVTSAAILHTFDLATMHADEVDFTASTELEPNLHKPSSKSENGEIETSWCHGVVLW 540

Query: 541 FETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASP 600
           FETGFTSRFCKES AVLSTSPYTPKTHWSQTILTFREPIAITS KSA+NKSA VGS ASP
Sbjct: 541 FETGFTSRFCKESPAVLSTSPYTPKTHWSQTILTFREPIAITSSKSATNKSATVGSLASP 600

Query: 601 AVKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           A+KVHLRLSIVRAP+HRSIDISLETTGIAGDGRK HWPVQLFTLR
Sbjct: 601 AIKVHLRLSIVRAPKHRSIDISLETTGIAGDGRKHHWPVQLFTLR 645

BLAST of Spg009657 vs. ExPASy TrEMBL
Match: A0A6J1JS79 (probable protein arginine N-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111487894 PE=4 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 551/644 (85.56%), Postives = 584/644 (90.68%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIEREEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCLF 60
           M++FP +LD+E C TEK EREE++DD+DDEQEDG+DDWDDWNADEE  EEAFDSNFLCLF
Sbjct: 1   MNRFPNDLDEEICITEKFEREEDDDDDDDEQEDGEDDWDDWNADEEGSEEAFDSNFLCLF 60

Query: 61  CNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLY 120
           CNSKY+DCDTLF HCNSVHYFDFH IRR LGLDFYGSFK+INYIRSQVAENRCWSCG L 
Sbjct: 61  CNSKYNDCDTLFNHCNSVHYFDFHGIRRTLGLDFYGSFKVINYIRSQVAENRCWSCGFLC 120

Query: 121 QSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLMN----- 180
           QSNQDLQNHLHKT NLSD+KA WDDDKYL PFRQDDPLLYSFAEDEEDD+ISL+N     
Sbjct: 121 QSNQDLQNHLHKTLNLSDVKAFWDDDKYLNPFRQDDPLLYSFAEDEEDDEISLINNDLQR 180

Query: 181 NTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTE 240
           N  TDGGNCN DEM+ E+LPFHPN++KENGMEDC S+ H + NAP NLEA IENTVKTTE
Sbjct: 181 NAGTDGGNCNHDEMSRENLPFHPNQNKENGMEDCCSSLHGYLNAPSNLEAEIENTVKTTE 240

Query: 241 ALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTI 300
            +GL     +D+QLKI RRN    DIKNAN NYF SYSSFGIHREMLSDKVR EAYM  I
Sbjct: 241 IVGL-----KDNQLKIARRNVDRMDIKNANVNYFDSYSSFGIHREMLSDKVRTEAYMHAI 300

Query: 301 LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNR 360
           LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRV+AVEASEKMAAVATQIAKDNGL RN 
Sbjct: 301 LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVLAVEASEKMAAVATQIAKDNGLWRNE 360

Query: 361 KQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARD 420
           KQTGD  +SSGTIEVVHGMVEELDKNI+IQPHSVDVL+SEWMGYCLLYESML+SVLFARD
Sbjct: 361 KQTGDYGVSSGTIEVVHGMVEELDKNIKIQPHSVDVLVSEWMGYCLLYESMLNSVLFARD 420

Query: 421 RWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVD 480
           RWLKPGGAILPDTATILVAGFG GGTSLPFWENVYGFSMSCVGKELV+DAAK PIVDIVD
Sbjct: 421 RWLKPGGAILPDTATILVAGFGAGGTSLPFWENVYGFSMSCVGKELVKDAAKAPIVDIVD 480

Query: 481 AKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWF 540
           A DLVTS AILHTFDLATMHPDEVDFTAST+LEPNLH PSSNS++VEVE SWCHGVVLWF
Sbjct: 481 ANDLVTSTAILHTFDLATMHPDEVDFTASTDLEPNLHYPSSNSDDVEVEASWCHGVVLWF 540

Query: 541 ETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPA 600
           ETGFTSRFCKES AVLSTSP+TPKTHWSQTILTF EPIAITSRKSA+NKSAPVGS ASPA
Sbjct: 541 ETGFTSRFCKESPAVLSTSPHTPKTHWSQTILTFTEPIAITSRKSATNKSAPVGSHASPA 600

Query: 601 VKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           VKVHLRLSIVRAPEHRSIDISLETTGIAGDGR R WPVQLFTLR
Sbjct: 601 VKVHLRLSIVRAPEHRSIDISLETTGIAGDGRNRDWPVQLFTLR 639

BLAST of Spg009657 vs. ExPASy TrEMBL
Match: A0A6J1H0C3 (probable protein arginine N-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111458867 PE=4 SV=1)

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 551/644 (85.56%), Postives = 582/644 (90.37%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIEREEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCLF 60
           MD+FP +LD+E C  EK EREE++DDE +EQEDGQDDWDDWNADEE  EEAFDSNFLCLF
Sbjct: 1   MDRFPNDLDEEICIAEKFEREEDDDDE-EEQEDGQDDWDDWNADEEGSEEAFDSNFLCLF 60

Query: 61  CNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLY 120
           CNSKY+DCD LF HCNSVHYFDFH IRR LGLDFYGSFK+INYIRSQVAENRCWSCG L 
Sbjct: 61  CNSKYNDCDALFNHCNSVHYFDFHGIRRTLGLDFYGSFKVINYIRSQVAENRCWSCGFLC 120

Query: 121 QSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLMN----- 180
           QSNQDLQNHLHKT NLSD+KALW+DDKYL PFRQDDPLLYSFAEDEEDD+ISL+N     
Sbjct: 121 QSNQDLQNHLHKTLNLSDVKALWNDDKYLNPFRQDDPLLYSFAEDEEDDEISLINNDLQR 180

Query: 181 NTDTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTE 240
           N  TDGGNCN DEM+ E+LPFHPN++KENGMEDC S+ H + NAP NLEA IENTVKTTE
Sbjct: 181 NAGTDGGNCNHDEMSRENLPFHPNQNKENGMEDCCSSLHGYLNAPSNLEAEIENTVKTTE 240

Query: 241 ALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTI 300
            LGL     +DDQLKI RRN    DIKNAN NYF SYSSFGIHREMLSDKVR EAYM  I
Sbjct: 241 ILGL-----KDDQLKIARRNVDRMDIKNANVNYFDSYSSFGIHREMLSDKVRTEAYMHAI 300

Query: 301 LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNR 360
           LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRV+AVEASEKMAAVATQIAKDNGL RN 
Sbjct: 301 LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVLAVEASEKMAAVATQIAKDNGLWRNG 360

Query: 361 KQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARD 420
           KQTGDC +SSGTIEVVHGMVEELDKNIEIQPHSVDVL+SEWMGYCLLYESML+SVLFARD
Sbjct: 361 KQTGDCGVSSGTIEVVHGMVEELDKNIEIQPHSVDVLVSEWMGYCLLYESMLNSVLFARD 420

Query: 421 RWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVD 480
           RWLKPGGAILPDTATILVAGFG GGTSLPFWENVYGFSMSCVGKEL++DAAK PIVDIVD
Sbjct: 421 RWLKPGGAILPDTATILVAGFGAGGTSLPFWENVYGFSMSCVGKELIKDAAKAPIVDIVD 480

Query: 481 AKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWF 540
           A DLVTS AILHTFDLATMHPDEVDFTAST+LEPNLH PSSNS++VEVE SWCHGVVLWF
Sbjct: 481 ANDLVTSTAILHTFDLATMHPDEVDFTASTDLEPNLHYPSSNSDDVEVEASWCHGVVLWF 540

Query: 541 ETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPA 600
           ETGFTSRFCKES AVLSTSP+TPKTHWSQTILTF EPIA+TSRKSA+NKSAPVGS ASPA
Sbjct: 541 ETGFTSRFCKESPAVLSTSPHTPKTHWSQTILTFTEPIALTSRKSATNKSAPVGSHASPA 600

Query: 601 VKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           VKVHLRLSIVRAPEHRSIDISLETTGIA DGR R WPVQLFTLR
Sbjct: 601 VKVHLRLSIVRAPEHRSIDISLETTGIAADGRNRDWPVQLFTLR 638

BLAST of Spg009657 vs. ExPASy TrEMBL
Match: A0A6J1CC36 (probable protein arginine N-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111010201 PE=4 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 548/644 (85.09%), Postives = 585/644 (90.84%), Query Frame = 0

Query: 1   MDQFPKELDKEFCNTEKIEREEEEDDEDDEQEDGQDDWDDWNADEEEGEEAFDSNFLCLF 60
           MD+F KELDKE C  EK  REEEE+D D+EQEDG DDWDDWNADEEEGE  FDSNFLCLF
Sbjct: 1   MDKFHKELDKETCKAEKFVREEEEED-DEEQEDGTDDWDDWNADEEEGEGEFDSNFLCLF 60

Query: 61  CNSKYSDCDTLFRHCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLY 120
           CNSKY+DCDTLF HCNSVH+FDF  IRRALGLDFYGSFKIINYIRSQVAENRCWSCGL  
Sbjct: 61  CNSKYNDCDTLFNHCNSVHHFDFRGIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLSC 120

Query: 121 QSNQDLQNHLHKTFNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLMNNT--- 180
           QSNQDLQNHLHKT NL+ +KA WDDDKYLKPFR+DDPLLYSFAEDEEDDDISL NN    
Sbjct: 121 QSNQDLQNHLHKTVNLNSMKAPWDDDKYLKPFREDDPLLYSFAEDEEDDDISLTNNNLGR 180

Query: 181 --DTDGGNCNVDEMTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTE 240
             D+DGGNCN DEMTGESLPFH N+ +ENG++DCSSTSH + N P NLEAVI++TVKTTE
Sbjct: 181 NMDSDGGNCNADEMTGESLPFHHNQIEENGIKDCSSTSHGYLNPPSNLEAVIQSTVKTTE 240

Query: 241 ALGLNDKMQEDDQLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTI 300
            LGLNDKM EDDQLK+ RR+ YSKDIKN N++YFGSYSSFGIHREMLSDKVRMEAY Q I
Sbjct: 241 TLGLNDKMLEDDQLKVSRRSLYSKDIKNVNDSYFGSYSSFGIHREMLSDKVRMEAYGQAI 300

Query: 301 LQNPSLFHNAAVMDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNR 360
           LQN SLFH A VMDVGCGTGILSLFAAKAGASRVIAVEAS+KMAAVATQIAK+NGL  NR
Sbjct: 301 LQNQSLFHEAVVMDVGCGTGILSLFAAKAGASRVIAVEASDKMAAVATQIAKENGLLWNR 360

Query: 361 KQTGDCSMSSGTIEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARD 420
           KQTGD SMSSG +EVVH MVEEL+KN+EIQP SVDVLLSEWMGYCLLYESMLSSVL ARD
Sbjct: 361 KQTGDSSMSSGIVEVVHSMVEELEKNVEIQPRSVDVLLSEWMGYCLLYESMLSSVLLARD 420

Query: 421 RWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVD 480
           RWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELV DAA  PIVDIVD
Sbjct: 421 RWLKPGGAILPDTATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVRDAANIPIVDIVD 480

Query: 481 AKDLVTSAAILHTFDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWF 540
           A DLVTSAAILHTFDLATMH D+VDFTA+TELEP+LH PS + ++VEVE SWCHGVVLWF
Sbjct: 481 ANDLVTSAAILHTFDLATMHLDDVDFTANTELEPSLHYPSKSGDDVEVEVSWCHGVVLWF 540

Query: 541 ETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPA 600
           ETGFTSRFCKES AVLSTSPYTPKTHWSQTILTFREPIA+TSRKSA+N+SAPVG+DASPA
Sbjct: 541 ETGFTSRFCKESPAVLSTSPYTPKTHWSQTILTFREPIAVTSRKSATNESAPVGTDASPA 600

Query: 601 VKVHLRLSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFTLR 640
           VKVHLR+SIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLF+LR
Sbjct: 601 VKVHLRVSIVRAPEHRSIDISLETTGIAGDGRKRHWPVQLFSLR 643

BLAST of Spg009657 vs. TAIR 10
Match: AT3G12270.1 (protein arginine methyltransferase 3 )

HSP 1 Score: 630.2 bits (1624), Expect = 1.8e-180
Identity = 345/632 (54.59%), Postives = 437/632 (69.15%), Query Frame = 0

Query: 24  EDDEDDEQEDGQDDWDDWNAD-------EEEGEEAFD---SNFLCLFCNSKYSDCDTLFR 83
           ED+E++  ++G  DW DW AD       EEE E+  D   S+FLCLFC+S +  CD LF 
Sbjct: 15  EDEEENYSDEG--DWGDWKADDNGIEGGEEEEEDDGDDSESDFLCLFCDSHFVSCDLLFE 74

Query: 84  HCNSVHYFDFHDIRRALGLDFYGSFKIINYIRSQVAENRCWSCGLLYQSNQDLQNHLHKT 143
           HC   H FDFH +R+ L LDFY SFK+INYIRSQVAEN C+S  +             + 
Sbjct: 75  HCRLSHGFDFHGVRKELKLDFYSSFKLINYIRSQVAENMCFSWKI-------------EA 134

Query: 144 FNLSDIKALWDDDKYLKPFRQDDPLLYSFAEDEEDDDISLMNNTDTDG----GNCNVD-E 203
            +  D+K  WD++KYLKPF Q+D LLYSFA+DEED++++       +     G+ ++D E
Sbjct: 135 DDYKDVKFPWDEEKYLKPFWQEDSLLYSFADDEEDEEVTFDREEVMEELQKLGDLSIDVE 194

Query: 204 MTGESLPFHPNRHKENGMEDCSSTSHSFSNAPRNLEAVIENTVKTTEALGLNDKMQEDDQ 263
             GES   + ++   NG +D +S S+       + + +I N               +D +
Sbjct: 195 ALGESSMSNSDKCNINGSKDVTSLSNCNGLKQSSADDLIVN--------------GKDAE 254

Query: 264 LKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVM 323
            K+      +++I+  NENYFGSYSSFGIHREMLSDKVR EAY   +L+NP+L + + VM
Sbjct: 255 PKVCDGRLVNRNIRKVNENYFGSYSSFGIHREMLSDKVRTEAYRDALLKNPTLLNGSVVM 314

Query: 324 DVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTI 383
           DVGCGTGILSLFAAKAGASRV+AVEASEKMA VAT+IAKDN +  + +        +G +
Sbjct: 315 DVGCGTGILSLFAAKAGASRVVAVEASEKMAKVATKIAKDNKVFNDNEH-------NGVL 374

Query: 384 EVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDT 443
           EV H MVEELDK+I+IQPHSVDVL+SEWMGYCLLYESMLSSVL+ARDRWLKPGGAILPDT
Sbjct: 375 EVAHSMVEELDKSIQIQPHSVDVLVSEWMGYCLLYESMLSSVLYARDRWLKPGGAILPDT 434

Query: 444 ATILVAGFGVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHT 503
           AT+ VAGFG G TSLPFWE+VYGF MS +GKE+ +D  + PIVD++  +DLVT   +L T
Sbjct: 435 ATMFVAGFGKGATSLPFWEDVYGFDMSSIGKEIHDDTTRLPIVDVIAERDLVTQPTLLQT 494

Query: 504 FDLATMHPDEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESS 563
           FDLATM PDEVDFTA+  LEP            E +T  CHGVVLWF+TGFTSRFCKE+ 
Sbjct: 495 FDLATMKPDEVDFTATATLEPT---------ESEAKTRLCHGVVLWFDTGFTSRFCKENP 554

Query: 564 AVLSTSPYTPKTHWSQTILTFREPIAI--TSRKSASNKSAPVGSDASPAVKVHLRLSIVR 623
            VLSTSPYTP THW+QTILTF+EPI++   S  S +++   +G++  PA  +HLR+S+ R
Sbjct: 555 TVLSTSPYTPPTHWAQTILTFQEPISVAPASVLSGNDRREAIGTEECPASSIHLRVSVAR 601

Query: 624 APEHRSIDISLETTGIAGDGRKRHWPVQLFTL 639
           A EHRSIDISLE TG++  G+KRHWPVQ+F L
Sbjct: 615 AHEHRSIDISLEATGLSSKGQKRHWPVQIFNL 601

BLAST of Spg009657 vs. TAIR 10
Match: AT2G19670.1 (protein arginine methyltransferase 1A )

HSP 1 Score: 204.5 bits (519), Expect = 2.5e-52
Identity = 134/357 (37.54%), Postives = 189/357 (52.94%), Query Frame = 0

Query: 260 DIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVMDVGCGTGILSL 319
           DI +A + YF SYS FGIH EML D VR ++Y   I +N  L  +  V+DVG GTGILSL
Sbjct: 41  DITSA-DYYFDSYSHFGIHEEMLKDVVRTKSYQDVIYKNKFLIKDKIVLDVGAGTGILSL 100

Query: 320 FAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTIEVVHGMVEELD 379
           F AKAGA+ V AVE S+ MA  A +I K NG              S  I V+ G +EE  
Sbjct: 101 FCAKAGAAHVYAVECSQ-MADTAKEIVKSNGF-------------SDVITVLKGKIEE-- 160

Query: 380 KNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDTATILVAGF--- 439
             IE+    VDV++SEWMGY LLYE+ML +VL+AR++WL  GG +LPD A++ V      
Sbjct: 161 --IELPVPKVDVIISEWMGYFLLYENMLDTVLYARNKWLVDGGIVLPDKASLYVTAIEDA 220

Query: 440 GVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHTFDLATMHP 499
                 + FW++VYGF MSC+ +  + +    P+VD VD   +VT + +L T D++ M  
Sbjct: 221 HYKDDKVEFWDDVYGFDMSCIKRRAITE----PLVDTVDGNQIVTDSKLLKTMDISKMAA 280

Query: 500 DEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESSAVLSTSPY 559
            +  FTA  +L             V       H +V +F+  FT   C +     ST P 
Sbjct: 281 GDASFTAPFKL-------------VAQRNDHIHALVAYFDVSFT--MCHKKMG-FSTGPK 340

Query: 560 TPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPAVKVHLRLSIVRAPEHRSI 614
           +  THW QT+L   + + I   ++ +       +  +P   V ++LS     +H +I
Sbjct: 341 SRATHWKQTVLYLEDVLTICEGETITGSMTIAQNKKNPR-DVDIKLSYSLNGQHCNI 357

BLAST of Spg009657 vs. TAIR 10
Match: AT4G29510.1 (arginine methyltransferase 11 )

HSP 1 Score: 203.8 bits (517), Expect = 4.3e-52
Identity = 134/357 (37.54%), Postives = 187/357 (52.38%), Query Frame = 0

Query: 260 DIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAVMDVGCGTGILSL 319
           D   + + YF SYS FGIH EML D VR + Y   I QN  L  +  V+DVG GTGILSL
Sbjct: 64  DDTTSADYYFDSYSHFGIHEEMLKDVVRTKTYQNVIYQNKFLIKDKIVLDVGAGTGILSL 123

Query: 320 FAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGTIEVVHGMVEELD 379
           F AKAGA+ V AVE S+ MA +A +I K NG              S  I V+ G +EE  
Sbjct: 124 FCAKAGAAHVYAVECSQ-MADMAKEIVKANGF-------------SDVITVLKGKIEE-- 183

Query: 380 KNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPDTATI---LVAGF 439
             IE+    VDV++SEWMGY LL+E+ML SVL+ARD+WL  GG +LPD A++    +   
Sbjct: 184 --IELPTPKVDVIISEWMGYFLLFENMLDSVLYARDKWLVEGGVVLPDKASLHLTAIEDS 243

Query: 440 GVGGTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAAILHTFDLATMHP 499
                 + FW +VYGF MSC+ K+    A   P+VD VD   +VT + +L T D++ M  
Sbjct: 244 EYKEDKIEFWNSVYGFDMSCIKKK----AMMEPLVDTVDQNQIVTDSRLLKTMDISKMSS 303

Query: 500 DEVDFTASTELEPNLHSPSSNSENVEVETSWCHGVVLWFETGFTSRFCKESSAVLSTSPY 559
            +  FTA  +L             V     + H +V +F+  FT   C +     ST P 
Sbjct: 304 GDASFTAPFKL-------------VAQRNDYIHALVAYFDVSFT--MCHKLLG-FSTGPK 363

Query: 560 TPKTHWSQTILTFREPIAITSRKSASNKSAPVGSDASPAVKVHLRLSIVRAPEHRSI 614
           +  THW QT+L   + + I   ++ +   +   +  +P   + ++LS     +H  I
Sbjct: 364 SRATHWKQTVLYLEDVLTICEGETITGTMSVSPNKKNPR-DIDIKLSYSLNGQHCKI 381

BLAST of Spg009657 vs. TAIR 10
Match: AT3G20020.1 (protein arginine methyltransferase 6 )

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-51
Identity = 142/411 (34.55%), Postives = 206/411 (50.12%), Query Frame = 0

Query: 196 FHPNRHKENGMEDCSSTS-HSFSNAPRNLEA-------VIENTVKTTEALGLNDKMQEDD 255
           FH + H+E  +ED    S  SF  A +   A        + N ++ ++ LG +  ++  +
Sbjct: 11  FHGDHHRELELEDKQGPSLSSFGRAKKRSHAGARDPRGGLANVLRVSDQLGEHKSLETSE 70

Query: 256 QLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAV 315
                  +F        +  YF SY+  GIH EM+ D+ R E Y + I+Q+ SL     V
Sbjct: 71  SSPPPCTDF--------DVAYFHSYAHVGIHEEMIKDRARTETYREAIMQHQSLIEGKVV 130

Query: 316 MDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGT 375
           +DVGCGTGILS+F A+AGA RV AV+AS+ +A  A ++ K NGL             S  
Sbjct: 131 VDVGCGTGILSIFCAQAGAKRVYAVDASD-IAVQAKEVVKANGL-------------SDK 190

Query: 376 IEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPD 435
           + V+HG VE+++ + E     VDV++SEWMGY LLYESML SV+ ARDRWLKPGG ILP 
Sbjct: 191 VIVLHGRVEDVEIDEE-----VDVIISEWMGYMLLYESMLGSVITARDRWLKPGGLILPS 250

Query: 436 TATILVAGFGVG---GTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAA 495
            AT+ +A          S+ FW NVYG  MS + +   + A + P V+ +  ++++T   
Sbjct: 251 HATLYMAPISHPDRYSHSIDFWRNVYGIDMSAMMQLAKQCAFEEPSVESISGENVLTWPE 310

Query: 496 ILHTFDLATMHPDEVD--------------------FTASTELEPNLHSPSSNSENVEVE 555
           ++   D  T+   E+D                    F    E      SP+ N+    + 
Sbjct: 311 VVKHIDCKTIKIQELDSVTARYKFNSMMRAPMHGFAFWFDVEFSGPASSPAKNTSETSI- 370

Query: 556 TSWCHGVVLWFETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAI 576
            S    +    E     R     + VLSTSP +P THW QTI+ F +PI +
Sbjct: 371 ASGSSSISPSGEVNQKKRTNPSDALVLSTSPESPPTHWQQTIVYFYDPIDV 393

BLAST of Spg009657 vs. TAIR 10
Match: AT3G20020.2 (protein arginine methyltransferase 6 )

HSP 1 Score: 178.7 bits (452), Expect = 1.5e-44
Identity = 133/411 (32.36%), Postives = 191/411 (46.47%), Query Frame = 0

Query: 196 FHPNRHKENGMEDCSSTS-HSFSNAPRNLEA-------VIENTVKTTEALGLNDKMQEDD 255
           FH + H+E  +ED    S  SF  A +   A        + N ++ ++ LG +  ++  +
Sbjct: 11  FHGDHHRELELEDKQGPSLSSFGRAKKRSHAGARDPRGGLANVLRVSDQLGEHKSLETSE 70

Query: 256 QLKIGRRNFYSKDIKNANENYFGSYSSFGIHREMLSDKVRMEAYMQTILQNPSLFHNAAV 315
                  +F        +  YF SY+  GIH EM+ D+ R E Y + I+Q+ SL     V
Sbjct: 71  SSPPPCTDF--------DVAYFHSYAHVGIHEEMIKDRARTETYREAIMQHQSLIEGKVV 130

Query: 316 MDVGCGTGILSLFAAKAGASRVIAVEASEKMAAVATQIAKDNGLGRNRKQTGDCSMSSGT 375
           +DVGCGTGILS+F A+AGA RV AV+AS+    +A Q                       
Sbjct: 131 VDVGCGTGILSIFCAQAGAKRVYAVDASD----IAVQ----------------------- 190

Query: 376 IEVVHGMVEELDKNIEIQPHSVDVLLSEWMGYCLLYESMLSSVLFARDRWLKPGGAILPD 435
                        ++EI    VDV++SEWMGY LLYESML SV+ ARDRWLKPGG ILP 
Sbjct: 191 -------------DVEID-EEVDVIISEWMGYMLLYESMLGSVITARDRWLKPGGLILPS 250

Query: 436 TATILVAGFGVG---GTSLPFWENVYGFSMSCVGKELVEDAAKGPIVDIVDAKDLVTSAA 495
            AT+ +A          S+ FW NVYG  MS + +   + A + P V+ +  ++++T   
Sbjct: 251 HATLYMAPISHPDRYSHSIDFWRNVYGIDMSAMMQLAKQCAFEEPSVESISGENVLTWPE 310

Query: 496 ILHTFDLATMHPDEVD--------------------FTASTELEPNLHSPSSNSENVEVE 555
           ++   D  T+   E+D                    F    E      SP+ N+    + 
Sbjct: 311 VVKHIDCKTIKIQELDSVTARYKFNSMMRAPMHGFAFWFDVEFSGPASSPAKNTSETSI- 370

Query: 556 TSWCHGVVLWFETGFTSRFCKESSAVLSTSPYTPKTHWSQTILTFREPIAI 576
            S    +    E     R     + VLSTSP +P THW QTI+ F +PI +
Sbjct: 371 ASGSSSISPSGEVNQKKRTNPSDALVLSTSPESPPTHWQQTIVYFYDPIDV 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022966971.10.0e+0086.55probable protein arginine N-methyltransferase 3 isoform X1 [Cucurbita maxima][more]
KAG7012865.10.0e+0085.63putative protein arginine N-methyltransferase 3, partial [Cucurbita argyrosperma... [more]
XP_022945828.10.0e+0086.36probable protein arginine N-methyltransferase 3 isoform X1 [Cucurbita moschata][more]
XP_022991200.10.0e+0085.56probable protein arginine N-methyltransferase 3 [Cucurbita maxima][more]
XP_023542552.10.0e+0086.20probable protein arginine N-methyltransferase 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q0WVD62.6e-17954.59Probable protein arginine N-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 ... [more]
A3BMN93.9e-16751.84Probable protein arginine N-methyltransferase 3 OS=Oryza sativa subsp. japonica ... [more]
A2YP566.2e-16551.36Probable protein arginine N-methyltransferase 3 OS=Oryza sativa subsp. indica OX... [more]
O704679.6e-5731.98Protein arginine N-methyltransferase 3 OS=Rattus norvegicus OX=10116 GN=Prmt3 PE... [more]
O606784.9e-5331.12Protein arginine N-methyltransferase 3 OS=Homo sapiens OX=9606 GN=PRMT3 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1HQS30.0e+0086.55probable protein arginine N-methyltransferase 3 isoform X1 OS=Cucurbita maxima O... [more]
A0A6J1G2320.0e+0086.36probable protein arginine N-methyltransferase 3 isoform X1 OS=Cucurbita moschata... [more]
A0A6J1JS790.0e+0085.56probable protein arginine N-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1H0C30.0e+0085.56probable protein arginine N-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1CC360.0e+0085.09probable protein arginine N-methyltransferase 3 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT3G12270.11.8e-18054.59protein arginine methyltransferase 3 [more]
AT2G19670.12.5e-5237.54protein arginine methyltransferase 1A [more]
AT4G29510.14.3e-5237.54arginine methyltransferase 11 [more]
AT3G20020.12.1e-5134.55protein arginine methyltransferase 6 [more]
AT3G20020.21.5e-4432.36protein arginine methyltransferase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041661ZN622/Rei1/Reh1, zinc finger C2H2-typePFAMPF12756zf-C2H2_2coord: 58..132
e-value: 4.7E-8
score: 33.3
NoneNo IPR availableGENE3D2.70.160.11coord: 429..630
e-value: 2.2E-40
score: 140.5
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 256..426
e-value: 1.8E-57
score: 195.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..41
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..41
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availablePANTHERPTHR11006:SF89PROTEIN ARGININE N-METHYLTRANSFERASE 3-RELATEDcoord: 26..637
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 307..425
e-value: 1.301E-11
score: 59.751
IPR041698Methyltransferase domain 25PFAMPF13649Methyltransf_25coord: 307..422
e-value: 1.0E-9
score: 38.9
IPR025799Protein arginine N-methyltransferasePANTHERPTHR11006PROTEIN ARGININE N-METHYLTRANSFERASEcoord: 26..637
IPR025799Protein arginine N-methyltransferasePROSITEPS51678SAM_MT_PRMTcoord: 265..593
score: 77.008492
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 58..79
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 271..577
IPR036236Zinc finger C2H2 superfamilySUPERFAMILY57667beta-beta-alpha zinc fingerscoord: 33..170

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009657.1Spg009657.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035246 peptidyl-arginine N-methylation
biological_process GO:0018216 peptidyl-arginine methylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016274 protein-arginine N-methyltransferase activity