Spg009624 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009624
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAspergillus nuclease S(1)
Locationscaffold7: 4239490 .. 4241648 (+)
RNA-Seq ExpressionSpg009624
SyntenySpg009624
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATGGATCGGCATGAGTGTGTTACATTACTAGACTCGTGTGAAAACAGCAAAAGCTCCCTTTTCTTTCCAGGAATGGAGAGAGTAACGGTTCCATTATTTTAAAACTTTACCTTAATTTCTTCCGCAAATGAATAAGACCCTTTTGGAATCCAGCTTCACCCAAACATGACTTCACAAGGAAAGTGAAAAGCACTTTCTATACTCAACCCAATCCCATCCTCATACACTTCACACTTTTGAGTGTCCTTCTCTTGATCGCTCTATTTTAGAAGATACAAACACAAACAGAAGAATAATAACAAAATCCAAAGATGGAGCCTTCCAGAGTTCTAATGGTGGGATTGGTCTCGTTGGCGTTCACCTTTCCTGTTAGCTTTGGTTGGGGCATTGACGGCCATTTCACCGTTTGCAGAATCGCTCAGGTTCTTCTCTCATCACTTTCTTTGAAACCCATTTCCCAAATTTACCTCTTTGATCAAGATTTTCATCTTTATTTTGCTGCAACAGTCACGGTTGAGCAAAGCTGCAGCAGATGCAGTGCAGCAACTGTTGCCGGACTATGCAAATGGTGACTTAGCGAGCGTTTGCATTTGGGCTGATAGAGTCAAGTTTCATTATCACTGGTCCTCTGCTCTTCATTTCATTGATACTCCAGATTCCCTTTGCACTTACCAATATGACAGTTGAGTTACTTTGCCTCATCCCCCTCTCTGTTCTAATTTCTGCAAGCCTTCTTCTCTTCTGGTTCTAAATTTTTATCATTTGAATCTCAAGGGGACTGCAAAGACGAAGCTGGAGAGAAAGGACGGTGCGTTGCAGGGGCCATTAGCAACTATACTTCCCAGCTTCTAAGCTACAAAACTCAACCTTCTCATTCTGAATGTAATTAGCAAGAAGATGAGAATCTTATAAACTTCGCTTCTATGATTAACTGATAAAATCGCTAATACGCTAAATTTTTGCATCACCAGATAACCTGACTGAAGCACTACTTTTCCTTTCACATTTCATGGGAGACATTCATCAGGTATGATATGAAGTTTATGTACATATTGTATGCAATTAGTTACTGAGATGAACTTACGGAAAATGCAGCCTCTGCATGTGGGCTTTACCGGAGACAGGGGAGGAAACACAATTGACGTTCACTGGTACACCAGGAAGCAAAATCTTCACCATGTAAATATAATAAACCAACTTCATCTGTGTGCCCTGGTAATTTCCAGTTTTGGATCAATAAAAATAAAGAGTTGTTCGTGATTCTTACAGATATGGGATAGCAACATAATCGAGACGGCAGAAGAAAAGTTCTATGGTTTCAGTGTGGATGGCCTGATTGATGCAATCAAAACGAATATTACGGTCATATAATTCTAAGTCCAAATCAACTCGAGAAACAAAGCTTGAAGTTTGATTCTCATCATTTCTTAGCAATTCTAATGCATTTTCTTCCTTTTGCTGTTTCTGATCAACAGAATGAATGGGCAAATCAAGTTGAAGAATGGGAAAAGTGTAGTTCTAATGAGATAGCATGCACAAAAATGTATTTGCCATAAGAAACAGTCTTGATCCTTTAATTTTAGAGCATTAAGTTCCAATAATTTACCCACAGTTTTCACTGAAATATGCAGATATGCATCAGAAAGTATCCAAGCTGCCTGTGACTGGGCATACAAAGGTGTAACTGAAGGTTCTACACTAGGAGGTAGATTGCTTACAAAGCTTGGTTGAGTTGTTCTTTGAGCAGAAGAATTTTTAATTTCAAAAAATTGTCTGCAGAGAAATATTTCTTCTCCCGTTTGCCAGAAGTGAACCTGCGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGTTTTGAATCAAATCTTTGGTTGACAGAATCTCCTCTAGTTTATACTCTGAGGAAAGGCCTGCCTGAAATAGTTAAGTTTCAAGTTAGTGCTAAACAGATGACCTTACTATGCCTATTTAATCTTGTAAAACAATCAACACCATGAGGTGAATAAAGTTCTTCCTGGAATATCATTCCCCTTGACTAATTTACTTCTTTTTAATCTCTTTATCTAGTCCACGCCACTTTGAACTTAGCATCTTTCTTACCAACTACATTTCTGTCATTTCATACAAAAAGAGATAAAAGGAGCCATAGTGAAAGCACC

mRNA sequence

ATGGAGCCTTCCAGAGTTCTAATGGTGGGATTGGTCTCGTTGGCGTTCACCTTTCCTGTTAGCTTTGGTTGGGGCATTGACGGCCATTTCACCGTTTGCAGAATCGCTCAGTCACGGTTGAGCAAAGCTGCAGCAGATGCAGTGCAGCAACTGTTGCCGGACTATGCAAATGGTGACTTAGCGAGCGTTTGCATTTGGGCTGATAGAGTCAAGTTTCATTATCACTGGTCCTCTGCTCTTCATTTCATTGATACTCCAGATTCCCTTTGCACTTACCAATATGACAGGGACTGCAAAGACGAAGCTGGAGAGAAAGGACGGTGCGTTGCAGGGGCCATTAGCAACTATACTTCCCAGCTTCTAAGCTACAAAACTCAACCTTCTCATTCTGAATATAACCTGACTGAAGCACTACTTTTCCTTTCACATTTCATGGGAGACATTCATCAGCCTCTGCATGTGGGCTTTACCGGAGACAGGGGAGGAAACACAATTGACGTTCACTGGTACACCAGGAAGCAAAATCTTCACCATATATGGGATAGCAACATAATCGAGACGGCAGAAGAAAAGTTCTATGGTTTCAGTGTGGATGGCCTGATTGATGCAATCAAAACGAATATTACGAATGAATGGGCAAATCAAGTTGAAGAATGGGAAAAGTGTAGTTCTAATGAGATAGCATGCACAAAAATATATGCATCAGAAAGTATCCAAGCTGCCTGTGACTGGGCATACAAAGGTGTAACTGAAGGTTCTACACTAGGAGAGAAATATTTCTTCTCCCGTTTGCCAGAAGTGAACCTGCGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGTTTTGAATCAAATCTTTGGTTGA

Coding sequence (CDS)

ATGGAGCCTTCCAGAGTTCTAATGGTGGGATTGGTCTCGTTGGCGTTCACCTTTCCTGTTAGCTTTGGTTGGGGCATTGACGGCCATTTCACCGTTTGCAGAATCGCTCAGTCACGGTTGAGCAAAGCTGCAGCAGATGCAGTGCAGCAACTGTTGCCGGACTATGCAAATGGTGACTTAGCGAGCGTTTGCATTTGGGCTGATAGAGTCAAGTTTCATTATCACTGGTCCTCTGCTCTTCATTTCATTGATACTCCAGATTCCCTTTGCACTTACCAATATGACAGGGACTGCAAAGACGAAGCTGGAGAGAAAGGACGGTGCGTTGCAGGGGCCATTAGCAACTATACTTCCCAGCTTCTAAGCTACAAAACTCAACCTTCTCATTCTGAATATAACCTGACTGAAGCACTACTTTTCCTTTCACATTTCATGGGAGACATTCATCAGCCTCTGCATGTGGGCTTTACCGGAGACAGGGGAGGAAACACAATTGACGTTCACTGGTACACCAGGAAGCAAAATCTTCACCATATATGGGATAGCAACATAATCGAGACGGCAGAAGAAAAGTTCTATGGTTTCAGTGTGGATGGCCTGATTGATGCAATCAAAACGAATATTACGAATGAATGGGCAAATCAAGTTGAAGAATGGGAAAAGTGTAGTTCTAATGAGATAGCATGCACAAAAATATATGCATCAGAAAGTATCCAAGCTGCCTGTGACTGGGCATACAAAGGTGTAACTGAAGGTTCTACACTAGGAGAGAAATATTTCTTCTCCCGTTTGCCAGAAGTGAACCTGCGGTTGGCACAAGGTGGAGTCAGATTAGCAGCTGTTTTGAATCAAATCTTTGGTTGA

Protein sequence

MEPSRVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQAACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG
Homology
BLAST of Spg009624 vs. NCBI nr
Match: XP_038892859.1 (endonuclease 2 [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.4e-150
Identity = 253/287 (88.15%), Postives = 269/287 (93.73%), Query Frame = 0

Query: 1   MEPSRVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDL 60
           ME  RVL++ +VSL F FPVSFGWGIDGHFTVCRIAQSRLS+AA DAVQ+LLP YANGDL
Sbjct: 1   MESCRVLVMAIVSLVFIFPVSFGWGIDGHFTVCRIAQSRLSQAAEDAVQELLPHYANGDL 60

Query: 61  ASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQL 120
           ASVCIWADR+KF YHWSSALHFIDTPDSLCTYQYDRDCKDE GEKGRCVAGAI+NYTSQL
Sbjct: 61  ASVCIWADRIKFRYHWSSALHFIDTPDSLCTYQYDRDCKDEGGEKGRCVAGAINNYTSQL 120

Query: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHH+W
Sbjct: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHVW 180

Query: 181 DSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQA 240
           DSNIIET+E+KFY FSVDGLIDAI+TNIT EWA+QVEEWE C SNE+ CT+IYASESIQA
Sbjct: 181 DSNIIETSEKKFYDFSVDGLIDAIQTNITKEWADQVEEWENCGSNEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           ACDWAYKGV EGSTL EKYF SR+PEVNLRLAQGGVRLAA LN+IFG
Sbjct: 241 ACDWAYKGVYEGSTLAEKYFSSRVPEVNLRLAQGGVRLAAALNRIFG 287

BLAST of Spg009624 vs. NCBI nr
Match: XP_008446611.1 (PREDICTED: endonuclease 2 [Cucumis melo] >KAA0034565.1 endonuclease 2 [Cucumis melo var. makuwa] >TYK09118.1 endonuclease 2 [Cucumis melo var. makuwa])

HSP 1 Score: 532.3 bits (1370), Expect = 2.5e-147
Identity = 247/287 (86.06%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MEPSRVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDL 60
           ME  R LMV  +SL F FPVSFGWGIDGHFTVC+IAQSRLSKAAADAVQ+LLP++A GDL
Sbjct: 1   MEYCRFLMVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDL 60

Query: 61  ASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQL 120
           ASVCIWADRVKF YHWSS LHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAI+NYTSQL
Sbjct: 61  ASVCIWADRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L+Y TQPS+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHH+W
Sbjct: 121 LTYNTQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVW 180

Query: 181 DSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQA 240
           DSNIIETAE KFY FSVDGL+DAI+TNITNEWA+QVEEWEKC S+E+ CT+IYASESIQA
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           ACDWAYKGV+EGSTL E+YF SR+P +NLRLAQGGVRLAA LN+IFG
Sbjct: 241 ACDWAYKGVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIFG 287

BLAST of Spg009624 vs. NCBI nr
Match: XP_004135088.1 (endonuclease 2 [Cucumis sativus] >KAE8648791.1 hypothetical protein Csa_008616 [Cucumis sativus])

HSP 1 Score: 520.4 bits (1339), Expect = 9.9e-144
Identity = 244/287 (85.02%), Postives = 262/287 (91.29%), Query Frame = 0

Query: 1   MEPSRVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDL 60
           ME  R L+V  +SL F FPVSFGWGIDGHFTVC+IAQSRLSKAAADAVQ+LLP+ A GDL
Sbjct: 1   MEKCRFLIVAFLSLVFIFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPESAQGDL 60

Query: 61  ASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQL 120
           ASVCIWADRVKF Y WS  LHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAI+NYTSQL
Sbjct: 61  ASVCIWADRVKFRYRWSPPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L+Y  QPS+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW
Sbjct: 121 LTYNAQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180

Query: 181 DSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQA 240
           DSNIIETAE KFY FSVDGL+DAI+TNI NEWA+QVEEWEKC S+E+ CT+IYASESIQA
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNIKNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           ACDWAYKGV+EGSTL EKYF SR+P + LRLAQGGVRLAA LN+IFG
Sbjct: 241 ACDWAYKGVSEGSTLAEKYFASRVPVLKLRLAQGGVRLAAALNRIFG 287

BLAST of Spg009624 vs. NCBI nr
Match: XP_022150586.1 (endonuclease 2 [Momordica charantia])

HSP 1 Score: 497.7 bits (1280), Expect = 6.9e-137
Identity = 233/286 (81.47%), Postives = 255/286 (89.16%), Query Frame = 0

Query: 1   MEPSRVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDL 60
           ME  +VL+  L+ L F FPV  GWG+DGH  VC+IAQSRLS+AAADAVQQLLPD A+GDL
Sbjct: 1   METCKVLVAALLLLGFIFPVISGWGVDGHAAVCKIAQSRLSRAAADAVQQLLPDSASGDL 60

Query: 61  ASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQL 120
           ASVC WADRVKF+YHWSSALHFIDTPDSLC+YQYDRDCKD+AG  GRCVAGAI+NYTSQL
Sbjct: 61  ASVCSWADRVKFYYHWSSALHFIDTPDSLCSYQYDRDCKDQAGVIGRCVAGAINNYTSQL 120

Query: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L Y+ QPS S+YNLT+ALLFLSHFMGDIHQPLHVGFT DRGGNTIDVHWYTRKQNLHHIW
Sbjct: 121 LKYQAQPSQSKYNLTQALLFLSHFMGDIHQPLHVGFTTDRGGNTIDVHWYTRKQNLHHIW 180

Query: 181 DSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQA 240
           D +IIETAEEKFY FSVDGL+DAI+ NIT  W N VEEWEKCSSNE+ACT+IYASESIQA
Sbjct: 181 DDSIIETAEEKFYNFSVDGLVDAIQMNITKGWRNHVEEWEKCSSNEVACTEIYASESIQA 240

Query: 241 ACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIF 287
           ACDWAYKG +EGS L +KYFFSR P VNLRLAQGGVRLAAVLN+IF
Sbjct: 241 ACDWAYKGASEGSILEDKYFFSRAPIVNLRLAQGGVRLAAVLNRIF 286

BLAST of Spg009624 vs. NCBI nr
Match: XP_022945222.1 (endonuclease 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 490.3 bits (1261), Expect = 1.1e-134
Identity = 228/288 (79.17%), Postives = 259/288 (89.93%), Query Frame = 0

Query: 1   MEPSRVLMVG-LVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGD 60
           M+P R  M G LV L F FPVSFGWG+DGHFT+C+IAQSRLS+AAADAVQ+LLP+YANG+
Sbjct: 1   MKPCRAFMAGLLVFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGE 60

Query: 61  LASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQ 120
           LA+VCIWADR+KF Y WSSALH+++TP+SLCTYQY RDCKDE G +GRCVAGAI+NYTSQ
Sbjct: 61  LANVCIWADRIKFRYRWSSALHYVNTPESLCTYQYARDCKDENGVEGRCVAGAINNYTSQ 120

Query: 121 LLSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 180
           LL+YKT+ S S+YNLTEALLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHHI
Sbjct: 121 LLTYKTRSSSSQYNLTEALLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHI 180

Query: 181 WDSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQ 240
           WDSNIIETAEEKFY  SVDGLIDAI+TNIT EW ++VEEWEKC+S +IACT IYASESI+
Sbjct: 181 WDSNIIETAEEKFYDSSVDGLIDAIQTNITKEWTDEVEEWEKCNSKKIACTDIYASESIR 240

Query: 241 AACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           AACDWAYKGVTEG+TL  +YF +R+P V LRLAQGGVRLAAVLN IFG
Sbjct: 241 AACDWAYKGVTEGATLAAQYFSTRVPVVYLRLAQGGVRLAAVLNSIFG 288

BLAST of Spg009624 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 8.5e-114
Identity = 193/283 (68.20%), Postives = 233/283 (82.33%), Query Frame = 0

Query: 6   VLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVCI 65
           V+M+  V L +  P   GWG +GH  +C+IAQ+RL + AA AV++LLP+ A GDL+S+C+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 66  WADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLSYKT 125
           WADRVKF YHWSS LH+I+TPD+ C+YQY+RDCKDE+GEKGRCVAGAI NYT+QLLSYKT
Sbjct: 70  WADRVKFRYHWSSPLHYINTPDA-CSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKT 129

Query: 126 -QPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNI 185
              S S+YNLTEALLF+SHFMGDIHQPLHV +  D+GGNTI+VHWYTRK NLHHIWDSNI
Sbjct: 130 AASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNI 189

Query: 186 IETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQAACDW 245
           IETAE   Y  +++G++DA+K NIT EWA+QV+ WE C + + AC  IYASE IQAACDW
Sbjct: 190 IETAEADLYNSALEGMVDALKKNITTEWADQVKRWETC-TKKTACPDIYASEGIQAACDW 249

Query: 246 AYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           AYKGVTEG TL ++YF+SRLP V  RLAQGGVRLAA LN+IFG
Sbjct: 250 AYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIFG 290

BLAST of Spg009624 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 8.0e-88
Identity = 151/266 (56.77%), Postives = 191/266 (71.80%), Query Frame = 0

Query: 24  WGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVCIWADRVKFH--YHWSSALH 83
           WG +GH+TVC+IA+S   +    AV++LLP  A+GDLASVC W D +K H  + W+S LH
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 84  FIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLS-YKTQPSHSEYNLTEALLF 143
           ++DTPD  C Y+Y RDC D    + RCV GAI NYT QL+S  +   +   YNLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 144 LSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNIIETAEEKFYGFSVDGL 203
           LSHF+GDIHQPLHVGF GD GGNTI V WY RK NLHH+WD+ IIE+A + +Y  S+  +
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 204 IDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQAACDWAYKGVTEGSTLGEKYF 263
           I+A++ N+TN+W+N V  WE C  N+ AC   YASESI  AC +AY+  T G+TLG+ YF
Sbjct: 205 IEALQANLTNDWSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDYF 264

Query: 264 FSRLPEVNLRLAQGGVRLAAVLNQIF 287
            SRLP V  RLAQGG+RLAA LN+IF
Sbjct: 265 LSRLPIVEKRLAQGGIRLAATLNRIF 290

BLAST of Spg009624 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.6e-83
Identity = 148/284 (52.11%), Postives = 193/284 (67.96%), Query Frame = 0

Query: 6   VLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVCI 65
           +L++G++ L     V   W  +GH   CRIAQ+ L    A  V+ LLPDY  GDL+++C+
Sbjct: 12  ILVLGILILCSVSSVR-SWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCV 71

Query: 66  WADRVK--FHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLSY 125
           W D+++  + Y W+S LH+IDTPD  C+Y+Y RDC D+ G K  CV GAI N+TSQL  Y
Sbjct: 72  WPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHY 131

Query: 126 KTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSN 185
               S   YN+TEALLFLSHFMGDIHQP+HVGFT D GGNTID+ WY  K NLHH+WD  
Sbjct: 132 GEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDRE 191

Query: 186 IIETAEEKFYGFSVDGLIDAIKTNITNE-WANQVEEWEKCSSNEIACTKIYASESIQAAC 245
           II TA ++ Y  ++D L + ++ NITN  W + +  W +C ++ IAC   YASESI+ AC
Sbjct: 192 IILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTEC-NDLIACPHKYASESIKLAC 251

Query: 246 DWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIF 287
            W YKGV  G TL E+YF +RLP V  R+ QGGVRLA +LN++F
Sbjct: 252 KWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVF 293

BLAST of Spg009624 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.8e-80
Identity = 147/285 (51.58%), Postives = 187/285 (65.61%), Query Frame = 0

Query: 5   RVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVC 64
           R+ +V ++ L      +  WG  GH+ VC+IAQS   +    AV++LLP+ ANG+LA+VC
Sbjct: 6   RMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVC 65

Query: 65  IWADRVKF--HYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLS 124
            W D +K    + W+SALHF DTPD  C Y+Y RDC      K  CV GAI NYT+QL+S
Sbjct: 66  SWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQLMS 125

Query: 125 -YKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWD 184
             +   S   YNLTEAL+FLSH+MGDIHQPLH GF GD GGN I VHWY ++ NLH +WD
Sbjct: 126 TSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWD 185

Query: 185 SNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQAA 244
             IIE+A E +Y  S+  +I  ++  + N W+N V  WE C  N+ AC   YASESI  A
Sbjct: 186 DMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASESIDLA 245

Query: 245 CDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIF 287
           C +AY+  T G+TLG+ YF SRLP V  RLAQGG+RLA  LN+IF
Sbjct: 246 CKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIF 285

BLAST of Spg009624 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 6.8e-79
Identity = 140/286 (48.95%), Postives = 189/286 (66.08%), Query Frame = 0

Query: 5   RVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYAN-GDLASV 64
           R+ +V ++ L      +  WG DGH+TVC++A+         AV++LLP+  + G LA  
Sbjct: 2   RLWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADF 61

Query: 65  CIWADRVK--FHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLL 124
           C W D +K    + W+S LH+++TP+  C Y+Y RDC D    K  CV GAI NYT+QL+
Sbjct: 62  CSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLM 121

Query: 125 SYKTQPSH-SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 184
           S      +   YNLTEALLFLSH+MGD+HQPLH GF GD GGNTI V+WY  K NLHH+W
Sbjct: 122 SASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVW 181

Query: 185 DSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQA 244
           D+ II++A E +Y  S+  +I A++  + N W+N V  W+ C  ++ AC  +YASESI  
Sbjct: 182 DNMIIDSALETYYNSSLPHMIQALQAKLKNGWSNDVPSWKSCHFHQKACPNLYASESIDL 241

Query: 245 ACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIF 287
           AC +AY+  T G+TLG++YF SRLP V  RLAQGG+RLAA LN+IF
Sbjct: 242 ACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIF 287

BLAST of Spg009624 vs. ExPASy TrEMBL
Match: A0A5A7SZC9 (Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001090 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-147
Identity = 247/287 (86.06%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MEPSRVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDL 60
           ME  R LMV  +SL F FPVSFGWGIDGHFTVC+IAQSRLSKAAADAVQ+LLP++A GDL
Sbjct: 1   MEYCRFLMVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDL 60

Query: 61  ASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQL 120
           ASVCIWADRVKF YHWSS LHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAI+NYTSQL
Sbjct: 61  ASVCIWADRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L+Y TQPS+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHH+W
Sbjct: 121 LTYNTQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVW 180

Query: 181 DSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQA 240
           DSNIIETAE KFY FSVDGL+DAI+TNITNEWA+QVEEWEKC S+E+ CT+IYASESIQA
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           ACDWAYKGV+EGSTL E+YF SR+P +NLRLAQGGVRLAA LN+IFG
Sbjct: 241 ACDWAYKGVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIFG 287

BLAST of Spg009624 vs. ExPASy TrEMBL
Match: A0A1S3BFG6 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103489294 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-147
Identity = 247/287 (86.06%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MEPSRVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDL 60
           ME  R LMV  +SL F FPVSFGWGIDGHFTVC+IAQSRLSKAAADAVQ+LLP++A GDL
Sbjct: 1   MEYCRFLMVAFLSLVFFFPVSFGWGIDGHFTVCKIAQSRLSKAAADAVQELLPEFAQGDL 60

Query: 61  ASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQL 120
           ASVCIWADRVKF YHWSS LHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAI+NYTSQL
Sbjct: 61  ASVCIWADRVKFRYHWSSPLHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAINNYTSQL 120

Query: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L+Y TQPS+SEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTID+HWYTRKQNLHH+W
Sbjct: 121 LTYNTQPSNSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDIHWYTRKQNLHHVW 180

Query: 181 DSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQA 240
           DSNIIETAE KFY FSVDGL+DAI+TNITNEWA+QVEEWEKC S+E+ CT+IYASESIQA
Sbjct: 181 DSNIIETAEGKFYDFSVDGLVDAIQTNITNEWADQVEEWEKCGSDEVPCTEIYASESIQA 240

Query: 241 ACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           ACDWAYKGV+EGSTL E+YF SR+P +NLRLAQGGVRLAA LN+IFG
Sbjct: 241 ACDWAYKGVSEGSTLAERYFASRVPVLNLRLAQGGVRLAAALNRIFG 287

BLAST of Spg009624 vs. ExPASy TrEMBL
Match: A0A6J1DBY6 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111018689 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.3e-137
Identity = 233/286 (81.47%), Postives = 255/286 (89.16%), Query Frame = 0

Query: 1   MEPSRVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDL 60
           ME  +VL+  L+ L F FPV  GWG+DGH  VC+IAQSRLS+AAADAVQQLLPD A+GDL
Sbjct: 1   METCKVLVAALLLLGFIFPVISGWGVDGHAAVCKIAQSRLSRAAADAVQQLLPDSASGDL 60

Query: 61  ASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQL 120
           ASVC WADRVKF+YHWSSALHFIDTPDSLC+YQYDRDCKD+AG  GRCVAGAI+NYTSQL
Sbjct: 61  ASVCSWADRVKFYYHWSSALHFIDTPDSLCSYQYDRDCKDQAGVIGRCVAGAINNYTSQL 120

Query: 121 LSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIW 180
           L Y+ QPS S+YNLT+ALLFLSHFMGDIHQPLHVGFT DRGGNTIDVHWYTRKQNLHHIW
Sbjct: 121 LKYQAQPSQSKYNLTQALLFLSHFMGDIHQPLHVGFTTDRGGNTIDVHWYTRKQNLHHIW 180

Query: 181 DSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQA 240
           D +IIETAEEKFY FSVDGL+DAI+ NIT  W N VEEWEKCSSNE+ACT+IYASESIQA
Sbjct: 181 DDSIIETAEEKFYNFSVDGLVDAIQMNITKGWRNHVEEWEKCSSNEVACTEIYASESIQA 240

Query: 241 ACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIF 287
           ACDWAYKG +EGS L +KYFFSR P VNLRLAQGGVRLAAVLN+IF
Sbjct: 241 ACDWAYKGASEGSILEDKYFFSRAPIVNLRLAQGGVRLAAVLNRIF 286

BLAST of Spg009624 vs. ExPASy TrEMBL
Match: A0A6J1G0B6 (Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111449527 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 5.3e-135
Identity = 228/288 (79.17%), Postives = 259/288 (89.93%), Query Frame = 0

Query: 1   MEPSRVLMVG-LVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGD 60
           M+P R  M G LV L F FPVSFGWG+DGHFT+C+IAQSRLS+AAADAVQ+LLP+YANG+
Sbjct: 1   MKPCRAFMAGLLVFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGE 60

Query: 61  LASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQ 120
           LA+VCIWADR+KF Y WSSALH+++TP+SLCTYQY RDCKDE G +GRCVAGAI+NYTSQ
Sbjct: 61  LANVCIWADRIKFRYRWSSALHYVNTPESLCTYQYARDCKDENGVEGRCVAGAINNYTSQ 120

Query: 121 LLSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 180
           LL+YKT+ S S+YNLTEALLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHHI
Sbjct: 121 LLTYKTRSSSSQYNLTEALLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHI 180

Query: 181 WDSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQ 240
           WDSNIIETAEEKFY  SVDGLIDAI+TNIT EW ++VEEWEKC+S +IACT IYASESI+
Sbjct: 181 WDSNIIETAEEKFYDSSVDGLIDAIQTNITKEWTDEVEEWEKCNSKKIACTDIYASESIR 240

Query: 241 AACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           AACDWAYKGVTEG+TL  +YF +R+P V LRLAQGGVRLAAVLN IFG
Sbjct: 241 AACDWAYKGVTEGATLAAQYFSTRVPVVYLRLAQGGVRLAAVLNSIFG 288

BLAST of Spg009624 vs. ExPASy TrEMBL
Match: A0A6J1HU04 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111467455 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 3.8e-133
Identity = 226/288 (78.47%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MEPSRVLMVG-LVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGD 60
           M+P R  M G LV L F FPVSFGWG+DGHFT+C+IAQSRLS+AAADAVQ+LLP+YANG+
Sbjct: 1   MKPCRAFMAGLLVFLVFIFPVSFGWGLDGHFTICKIAQSRLSRAAADAVQRLLPEYANGE 60

Query: 61  LASVCIWADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQ 120
           LA+VCIWADR+KF Y WSSALH+++TP+SLCTYQY RDCKDE G +GRCVAGAI+NYTSQ
Sbjct: 61  LANVCIWADRIKFRYRWSSALHYVNTPESLCTYQYARDCKDENGVEGRCVAGAINNYTSQ 120

Query: 121 LLSYKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHI 180
           LL+YKT+ S S+YNLTEALLFLSHFMGDIHQPLHVGF  D+GGNTI+VHWYTRKQNLHHI
Sbjct: 121 LLTYKTRSSSSQYNLTEALLFLSHFMGDIHQPLHVGFAEDKGGNTIEVHWYTRKQNLHHI 180

Query: 181 WDSNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQ 240
           WDSNIIETAEEKFY  SV+GLIDAI+ NIT EW ++VEEW KC+S  IACT IYASESI+
Sbjct: 181 WDSNIIETAEEKFYDSSVNGLIDAIQMNITKEWTDEVEEWVKCNSKTIACTDIYASESIR 240

Query: 241 AACDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           AACDWAYKGVTEG+TL  KYF +R+P V LRLAQGGVRLAAVLN IFG
Sbjct: 241 AACDWAYKGVTEGATLAAKYFSTRVPVVYLRLAQGGVRLAAVLNSIFG 288

BLAST of Spg009624 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 411.4 bits (1056), Expect = 6.0e-115
Identity = 193/283 (68.20%), Postives = 233/283 (82.33%), Query Frame = 0

Query: 6   VLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVCI 65
           V+M+  V L +  P   GWG +GH  +C+IAQ+RL + AA AV++LLP+ A GDL+S+C+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 66  WADRVKFHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLSYKT 125
           WADRVKF YHWSS LH+I+TPD+ C+YQY+RDCKDE+GEKGRCVAGAI NYT+QLLSYKT
Sbjct: 70  WADRVKFRYHWSSPLHYINTPDA-CSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKT 129

Query: 126 -QPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNI 185
              S S+YNLTEALLF+SHFMGDIHQPLHV +  D+GGNTI+VHWYTRK NLHHIWDSNI
Sbjct: 130 AASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSNI 189

Query: 186 IETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQAACDW 245
           IETAE   Y  +++G++DA+K NIT EWA+QV+ WE C + + AC  IYASE IQAACDW
Sbjct: 190 IETAEADLYNSALEGMVDALKKNITTEWADQVKRWETC-TKKTACPDIYASEGIQAACDW 249

Query: 246 AYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIFG 288
           AYKGVTEG TL ++YF+SRLP V  RLAQGGVRLAA LN+IFG
Sbjct: 250 AYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIFG 290

BLAST of Spg009624 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 325.1 bits (832), Expect = 5.7e-89
Identity = 151/266 (56.77%), Postives = 191/266 (71.80%), Query Frame = 0

Query: 24  WGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVCIWADRVKFH--YHWSSALH 83
           WG +GH+TVC+IA+S   +    AV++LLP  A+GDLASVC W D +K H  + W+S LH
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 84  FIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLS-YKTQPSHSEYNLTEALLF 143
           ++DTPD  C Y+Y RDC D    + RCV GAI NYT QL+S  +   +   YNLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 144 LSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSNIIETAEEKFYGFSVDGL 203
           LSHF+GDIHQPLHVGF GD GGNTI V WY RK NLHH+WD+ IIE+A + +Y  S+  +
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 204 IDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQAACDWAYKGVTEGSTLGEKYF 263
           I+A++ N+TN+W+N V  WE C  N+ AC   YASESI  AC +AY+  T G+TLG+ YF
Sbjct: 205 IEALQANLTNDWSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDYF 264

Query: 264 FSRLPEVNLRLAQGGVRLAAVLNQIF 287
            SRLP V  RLAQGG+RLAA LN+IF
Sbjct: 265 LSRLPIVEKRLAQGGIRLAATLNRIF 290

BLAST of Spg009624 vs. TAIR 10
Match: AT1G11190.1 (bifunctional nuclease i )

HSP 1 Score: 310.8 bits (795), Expect = 1.1e-84
Identity = 148/284 (52.11%), Postives = 193/284 (67.96%), Query Frame = 0

Query: 6   VLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVCI 65
           +L++G++ L     V   W  +GH   CRIAQ+ L    A  V+ LLPDY  GDL+++C+
Sbjct: 12  ILVLGILILCSVSSVR-SWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCV 71

Query: 66  WADRVK--FHYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLSY 125
           W D+++  + Y W+S LH+IDTPD  C+Y+Y RDC D+ G K  CV GAI N+TSQL  Y
Sbjct: 72  WPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHY 131

Query: 126 KTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWDSN 185
               S   YN+TEALLFLSHFMGDIHQP+HVGFT D GGNTID+ WY  K NLHH+WD  
Sbjct: 132 GEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDRE 191

Query: 186 IIETAEEKFYGFSVDGLIDAIKTNITNE-WANQVEEWEKCSSNEIACTKIYASESIQAAC 245
           II TA ++ Y  ++D L + ++ NITN  W + +  W +C ++ IAC   YASESI+ AC
Sbjct: 192 IILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTEC-NDLIACPHKYASESIKLAC 251

Query: 246 DWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIF 287
            W YKGV  G TL E+YF +RLP V  R+ QGGVRLA +LN++F
Sbjct: 252 KWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVF 293

BLAST of Spg009624 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 300.1 bits (767), Expect = 2.0e-81
Identity = 147/285 (51.58%), Postives = 187/285 (65.61%), Query Frame = 0

Query: 5   RVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVC 64
           R+ +V ++ L      +  WG  GH+ VC+IAQS   +    AV++LLP+ ANG+LA+VC
Sbjct: 6   RMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVC 65

Query: 65  IWADRVKF--HYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLS 124
            W D +K    + W+SALHF DTPD  C Y+Y RDC      K  CV GAI NYT+QL+S
Sbjct: 66  SWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQLMS 125

Query: 125 -YKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWD 184
             +   S   YNLTEAL+FLSH+MGDIHQPLH GF GD GGN I VHWY ++ NLH +WD
Sbjct: 126 TSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWD 185

Query: 185 SNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQAA 244
             IIE+A E +Y  S+  +I  ++  + N W+N V  WE C  N+ AC   YASESI  A
Sbjct: 186 DMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASESIDLA 245

Query: 245 CDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIF 287
           C +AY+  T G+TLG+ YF SRLP V  RLAQGG+RLA  LN+IF
Sbjct: 246 CKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIF 285

BLAST of Spg009624 vs. TAIR 10
Match: AT4G21590.2 (endonuclease 3 )

HSP 1 Score: 300.1 bits (767), Expect = 2.0e-81
Identity = 147/285 (51.58%), Postives = 187/285 (65.61%), Query Frame = 0

Query: 5   RVLMVGLVSLAFTFPVSFGWGIDGHFTVCRIAQSRLSKAAADAVQQLLPDYANGDLASVC 64
           R+ +V ++ L      +  WG  GH+ VC+IAQS   +    AV++LLP+ ANG+LA+VC
Sbjct: 6   RMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVC 65

Query: 65  IWADRVKF--HYHWSSALHFIDTPDSLCTYQYDRDCKDEAGEKGRCVAGAISNYTSQLLS 124
            W D +K    + W+SALHF DTPD  C Y+Y RDC      K  CV GAI NYT+QL+S
Sbjct: 66  SWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQLMS 125

Query: 125 -YKTQPSHSEYNLTEALLFLSHFMGDIHQPLHVGFTGDRGGNTIDVHWYTRKQNLHHIWD 184
             +   S   YNLTEAL+FLSH+MGDIHQPLH GF GD GGN I VHWY ++ NLH +WD
Sbjct: 126 TSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWD 185

Query: 185 SNIIETAEEKFYGFSVDGLIDAIKTNITNEWANQVEEWEKCSSNEIACTKIYASESIQAA 244
             IIE+A E +Y  S+  +I  ++  + N W+N V  WE C  N+ AC   YASESI  A
Sbjct: 186 DMIIESALETYYNSSLPRMIHELQAKLKNGWSNDVPSWESCQLNQTACPNPYASESIDLA 245

Query: 245 CDWAYKGVTEGSTLGEKYFFSRLPEVNLRLAQGGVRLAAVLNQIF 287
           C +AY+  T G+TLG+ YF SRLP V  RLAQGG+RLA  LN+IF
Sbjct: 246 CKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIF 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892859.11.4e-15088.15endonuclease 2 [Benincasa hispida][more]
XP_008446611.12.5e-14786.06PREDICTED: endonuclease 2 [Cucumis melo] >KAA0034565.1 endonuclease 2 [Cucumis m... [more]
XP_004135088.19.9e-14485.02endonuclease 2 [Cucumis sativus] >KAE8648791.1 hypothetical protein Csa_008616 [... [more]
XP_022150586.16.9e-13781.47endonuclease 2 [Momordica charantia][more]
XP_022945222.11.1e-13479.17endonuclease 2 isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9C9G48.5e-11468.20Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1[more]
F4JJL08.0e-8856.77Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1[more]
Q9SXA61.6e-8352.11Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1[more]
Q8LDW62.8e-8051.58Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1[more]
F4JJL36.8e-7948.95Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SZC91.2e-14786.06Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3BFG61.2e-14786.06Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103489294 PE=3 SV=1[more]
A0A6J1DBY63.3e-13781.47Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111018689 PE=3 SV... [more]
A0A6J1G0B65.3e-13579.17Aspergillus nuclease S(1) OS=Cucurbita moschata OX=3662 GN=LOC111449527 PE=3 SV=... [more]
A0A6J1HU043.8e-13378.47Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111467455 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G68290.16.0e-11568.20endonuclease 2 [more]
AT4G21585.15.7e-8956.77endonuclease 4 [more]
AT1G11190.11.1e-8452.11bifunctional nuclease i [more]
AT4G21590.12.0e-8151.58endonuclease 3 [more]
AT4G21590.22.0e-8151.58endonuclease 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003154S1/P1 nucleasePFAMPF02265S1-P1_nucleasecoord: 24..286
e-value: 1.6E-81
score: 273.9
IPR003154S1/P1 nucleasePANTHERPTHR33146ENDONUCLEASE 4coord: 8..287
IPR003154S1/P1 nucleaseCDDcd11010S1-P1_nucleasecoord: 24..286
e-value: 5.7609E-91
score: 268.484
IPR008947Phospholipase C/P1 nuclease domain superfamilyGENE3D1.10.575.10P1 Nucleasecoord: 24..287
e-value: 1.6E-104
score: 351.4
IPR008947Phospholipase C/P1 nuclease domain superfamilySUPERFAMILY48537Phospholipase C/P1 nucleasecoord: 24..285
NoneNo IPR availablePANTHERPTHR33146:SF2ENDONUCLEASE 2coord: 8..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009624.1Spg009624.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006308 DNA catabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0004521 endoribonuclease activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000014 single-stranded DNA endodeoxyribonuclease activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds