Spg009623 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg009623 vs. NCBI nr
Match: XP_022138963.1 (WAT1-related protein At5g07050 [Momordica charantia])

HSP 1 Score: 679.1 bits (1751), Expect = 2.4e-191
Identity = 368/418 (88.04%), Postives = 382/418 (91.39%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSLS CN FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLSCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPFVLFFERRGQP+ITLKVF QIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFVLFFERRGQPKITLKVFTQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNN 180
           MTFVMAVIFRME LDMKK+RCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHS  S+ N
Sbjct: 121 MTFVMAVIFRMENLDMKKLRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSSNSSTN 180

Query: 181 NVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240
           +  +++ANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA
Sbjct: 181 S-DENSANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240

Query: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300
           VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII
Sbjct: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300

Query: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVA--DEIPEAIKAN 360
           VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKH+ENLE KSAS A  DEIPEAIKAN
Sbjct: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHRENLEIKSASAAADDEIPEAIKAN 360

Query: 361 NSS--QPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIPRE 415
           ++   QPNNNNN N              PNK    +NSLFI++ DPENPIKPNQ+PRE
Sbjct: 361 SNPNLQPNNNNNGN------------MGPNK---PSNSLFITISDPENPIKPNQVPRE 402

BLAST of Spg009623 vs. NCBI nr
Match: XP_022985721.1 (WAT1-related protein At5g07050-like [Cucurbita maxima])

HSP 1 Score: 659.1 bits (1699), Expect = 2.6e-185
Identity = 356/418 (85.17%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLS     SFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSF----SFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           I PFVLFFERRGQP+ITLK+FIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IVPFVLFFERRGQPKITLKIFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNN 180
           MTFVMAVIFRME LDMKK+RCQAK+LGTLVTV GAM+MTLYKGPLVQM WSKHSH S  N
Sbjct: 121 MTFVMAVIFRMENLDMKKLRCQAKVLGTLVTVGGAMVMTLYKGPLVQMPWSKHSHHS--N 180

Query: 181 NVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240
             SD  ANKDWFKGS+FLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCF+GTLQAIA
Sbjct: 181 QASDDKANKDWFKGSMFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFVGTLQAIA 240

Query: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300
           VTLVAEHKA+VWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII
Sbjct: 241 VTLVAEHKAAVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300

Query: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVA----DEIPEAIK 360
           VAIMGSFILAE IFLGGIIGSI IVFGLYSVLWGKH+ENLE K A+ A    +EIP+ IK
Sbjct: 301 VAIMGSFILAENIFLGGIIGSIFIVFGLYSVLWGKHRENLEIKVAAAAASSDEEIPQPIK 360

Query: 361 ANNSSQPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIPRE 415
               SQPNNNNNNNNIA                  NNS+FISMP PENPIKPNQIPRE
Sbjct: 361 L--PSQPNNNNNNNNIA------------------NNSIFISMPAPENPIKPNQIPRE 392

BLAST of Spg009623 vs. NCBI nr
Match: KAG6573768.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 656.0 bits (1691), Expect = 2.2e-184
Identity = 355/413 (85.96%), Postives = 377/413 (91.28%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSLS  +SF+ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLSCWSSFVENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPF  FFERR QP+ITLKVF QIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFAFFFERREQPKITLKVFTQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNN 180
           MTFVMAVIFRMEKL+MKK+RCQAK LGTLVTV GAMLMTLYKGPL+QMAWSKHSH  T++
Sbjct: 121 MTFVMAVIFRMEKLEMKKLRCQAKFLGTLVTVIGAMLMTLYKGPLIQMAWSKHSHVPTSS 180

Query: 181 NVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240
           N   ++ANKDWFKGSIFLIIATLAW+SLFVLQNQALKTY NHQ TLTTLVCF+GTLQAIA
Sbjct: 181 N-DQASANKDWFKGSIFLIIATLAWSSLFVLQNQALKTYMNHQLTLTTLVCFMGTLQAIA 240

Query: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300
           VT+VAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII
Sbjct: 241 VTVVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300

Query: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSA-SVADEIPEAIKANN 360
           VAIMGSFIL+EKI+LGGIIGSILIVFGLYSVLWGKHKENLE+KSA   A ++P+AIK   
Sbjct: 301 VAIMGSFILSEKIYLGGIIGSILIVFGLYSVLWGKHKENLETKSADDDAHKLPQAIKL-- 360

Query: 361 SSQPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIP 413
           SSQPNNNNNNNN+ NLE        PNKL   +NSL ISMPDPE+PIKPNQ P
Sbjct: 361 SSQPNNNNNNNNL-NLE--------PNKL---SNSLIISMPDPESPIKPNQNP 398

BLAST of Spg009623 vs. NCBI nr
Match: XP_038892785.1 (WAT1-related protein At5g07050 [Benincasa hispida])

HSP 1 Score: 653.3 bits (1684), Expect = 1.4e-183
Identity = 353/405 (87.16%), Postives = 365/405 (90.12%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSL+ CNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLNCCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           I PFVLFFERR QPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IVPFVLFFERREQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNN 180
           MTFVMAVIFRMEKL+MKK+RCQAK+LGTLV+VAGAMLMTLYKG L+QM WSKHSH  TN+
Sbjct: 121 MTFVMAVIFRMEKLEMKKLRCQAKVLGTLVSVAGAMLMTLYKGSLLQMPWSKHSHHLTNS 180

Query: 181 NVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240
           N  +++ NKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA
Sbjct: 181 N--ETSNNKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240

Query: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300
           VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII
Sbjct: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300

Query: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVADEIPEAIKANNS 360
           VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLE K+A   DEIPEAIK   S
Sbjct: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTADDDDEIPEAIKP--S 360

Query: 361 SQPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENP 406
           SQ N NNNNNN                   NNNS+FISMP PE P
Sbjct: 361 SQLNINNNNNN-------------------NNNSIFISMPTPEKP 382

BLAST of Spg009623 vs. NCBI nr
Match: XP_008445949.1 (PREDICTED: WAT1-related protein At5g07050 [Cucumis melo])

HSP 1 Score: 652.5 bits (1682), Expect = 2.4e-183
Identity = 352/415 (84.82%), Postives = 366/415 (88.19%), Query Frame = 0

Query: 1   MGKL--SLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT 60
           MGKL    + CN FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT
Sbjct: 1   MGKLISFFNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT 60

Query: 61  ASIAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML 120
           ASIAPFVLFFERRGQPR+T KVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML
Sbjct: 61  ASIAPFVLFFERRGQPRMTFKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML 120

Query: 121 PAMTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHAST 180
           PAMTFVMAVIFRMEKL+MKK+RCQAK+LGTLVTV GAMLMTLYKGP +QM WSK+SH + 
Sbjct: 121 PAMTFVMAVIFRMEKLEMKKLRCQAKVLGTLVTVGGAMLMTLYKGPPLQMPWSKNSHLT- 180

Query: 181 NNNVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQA 240
             N S+S+ NKDWFKGSIFLIIATLAWASLFVLQNQ LKTYKNHQFTLTTLVCF+GTLQA
Sbjct: 181 --NSSESSNNKDWFKGSIFLIIATLAWASLFVLQNQVLKTYKNHQFTLTTLVCFMGTLQA 240

Query: 241 IAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMM 300
           IAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMM
Sbjct: 241 IAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMM 300

Query: 301 IIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVAD-EIPEAIKA 360
           IIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKS S  D EIPE IKA
Sbjct: 301 IIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSGSDHDNEIPEVIKA 360

Query: 361 NNSSQPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIP 413
           +N+SQPN                        ISNNNS+FISMP PE P KPNQIP
Sbjct: 361 SNNSQPN------------------------ISNNNSIFISMPTPETPTKPNQIP 388

BLAST of Spg009623 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.1e-141
Identity = 259/385 (67.27%), Postives = 315/385 (81.82%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           M    +S C SFL +SKPYFAMISLQFGYAGMNII+K+SL+ GMSHYVLVVYRHA ATA 
Sbjct: 1   MSMEEISSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAV 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPF  FFER+ QP+IT  +F+Q+F+L LLGPVIDQNFYY GLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKH------S 180
           MTF++AV+FRME LD+KK+ CQAKI GT+VTVAGAMLMT+YKGP+V++ W+K+      S
Sbjct: 121 MTFILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSS 180

Query: 181 HASTNNNVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLG 240
           HA+T ++  +S+++K++ KGSI LI ATLAWASLFVLQ + LKTY  HQ +LTTL+CF+G
Sbjct: 181 HANTTSS-KNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIG 240

Query: 241 TLQAIAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFS 300
           TLQA+AVT V EH  S WRIGWDMNLLAAAY+GIV SSISYYVQG+VMKKRGPVFATAFS
Sbjct: 241 TLQAVAVTFVMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFS 300

Query: 301 PLMMIIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKEN----LESKSASVADE 360
           PLMM+IVA+MGSF+LAEKIFLGG+IG++LIV GLY+VLWGK KEN     E        +
Sbjct: 301 PLMMVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSK 360

Query: 361 IPEAIKANNSSQPNNNNNNNNIANL 376
           + E ++AN S    +  +N+ ++ +
Sbjct: 361 VTEDVEANGSKMKISEGDNSMLSTI 384

BLAST of Spg009623 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.2e-102
Identity = 184/326 (56.44%), Postives = 257/326 (78.83%), Query Frame = 0

Query: 14  ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRGQ 73
           E++KPYFAM+ LQFGYAGMN+++K  L RGMSHYVLV YR+AFATA+IAPF L  ER+ +
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 74  PRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRMEK 133
            ++T  +F++IF+LALLGPVIDQN YY GLK TSPTFS A+SN++PA+T ++A +FRMEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 134 LDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNNNVSDSNANKDWFK 193
           ++M+KVRC  K++GTLVTV G++LM  YKGP +    S  + AS       S    D+ K
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFFRSHLTAAS-------SPPTADYLK 186

Query: 194 GSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVTLVAEHKASVWR 253
            ++FL++A+L+WAS FVLQ   LK Y  H  +++T+VCF+GTLQ++A+  V EH  S   
Sbjct: 187 AAVFLLLASLSWASFFVLQAATLKKYSAH-LSMSTMVCFMGTLQSLALAFVMEHNPSALN 246

Query: 254 IGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILAEKI 313
           IG+DMNLLA+AYAGI++SSI+YYVQGL+M+++GPVF TAF+PL+++IV+IM  F+L + I
Sbjct: 247 IGFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGI 306

Query: 314 FLGGIIGSILIVFGLYSVLWGKHKEN 340
           +LGG+IG ++++ G+Y+VLWGKH ++
Sbjct: 307 YLGGVIGVVVLMVGVYAVLWGKHVDD 324

BLAST of Spg009623 vs. ExPASy Swiss-Prot
Match: Q9LXX8 (WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.9e-98
Identity = 187/339 (55.16%), Postives = 262/339 (77.29%), Query Frame = 0

Query: 14  ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRGQ 73
           E++KPYFAM+ LQFGYAGMN+++KV L RGMSHYVLV YR+AFATA+IAPF L  ER+ +
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 74  PRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRMEK 133
           P++T  +F+QIFVLALLGP+IDQN YYAGLK TSPTF+ A++N++PA+TF++++I RMEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 134 LDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNNNVSDSNANKDWFK 193
           ++M+KVR QAK++GTLV V GAMLM L+K PL+       SH + +   + S A +D+ K
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFL---RSHLTGH---ALSPAGEDYLK 186

Query: 194 GSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVTLVAEHKASVWR 253
            ++FL+IA+ +WAS FVLQ   LK Y +H  +L+T+VCF+GTLQ+ A+T V E   S W 
Sbjct: 187 ATVFLLIASFSWASFFVLQAATLKRYSSH-LSLSTMVCFMGTLQSTALTFVMEPNLSAWN 246

Query: 254 IGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILAEKI 313
           IG+DMNLLA+AYAGI++SSI+YYVQG++ K++  +F TAF+PL++II +I+G  IL + +
Sbjct: 247 IGFDMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLILNQTL 306

Query: 314 FLGGIIGSILIVFGLYSVLWGK-----HKENLESKSASV 348
            LGG++G  ++V G+ +VLWGK      +EN+E K   +
Sbjct: 307 NLGGVLGMAILVVGVCTVLWGKEGDIDEEENIEEKFVEI 338

BLAST of Spg009623 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 5.0e-91
Identity = 166/333 (49.85%), Postives = 236/333 (70.87%), Query Frame = 0

Query: 13  LENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRG 72
           + + KPY AMIS+QFGYAGM II+ VSL  GM+HYVL VYRHA ATA IAPF LF ER+ 
Sbjct: 6   MNSLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERKI 65

Query: 73  QPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRME 132
           +P++T ++F+QI +L  + PV+DQN YY G+ YTS TF+ A +N+LPA+TFV+A+IFR+E
Sbjct: 66  RPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRLE 125

Query: 133 KLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQM-------AWSKHSHASTNNNVSDS 192
            ++ KKVR  AK++GT++TV+GA+LMTLYKGP+V                  ++     +
Sbjct: 126 SVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAA 185

Query: 193 NANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVTLVA 252
             +K W  G++ L+  T  WA  F+LQ+  LK Y   + +LTTL+C +GTL+  AV+LV 
Sbjct: 186 AMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYP-AELSLTTLICLMGTLEGTAVSLVT 245

Query: 253 EHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMG 312
               S W+IG+D NL AAAY+G++ S ++YYVQG+VM++RGPVF   F+PL ++I A +G
Sbjct: 246 VRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALG 305

Query: 313 SFILAEKIFLGGIIGSILIVFGLYSVLWGKHKE 339
             +L+E I LG +IG++ I+ GLY+V+WGK K+
Sbjct: 306 VVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKD 337

BLAST of Spg009623 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.8e-88
Identity = 172/341 (50.44%), Postives = 238/341 (69.79%), Query Frame = 0

Query: 13  LENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRG 72
           L+  KP+  ++SLQFGYAG++II+K +L++GMS +VL  YRH  AT  IAPF  F +R+ 
Sbjct: 3   LKTWKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKI 62

Query: 73  QPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRME 132
           +P++TL +F +I +L LL P IDQN YY G+KYTS TF+ AM+N+LPA  F+MA IFR+E
Sbjct: 63  RPKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLE 122

Query: 133 KLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNNNVSDSNANKDWF 192
           K+++KK+  QAKILGT+VTV GAMLMT+ KGPL+ + W+  +    + + S++   +D  
Sbjct: 123 KVNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWA--NPHDIHQDSSNTGVKQDLT 182

Query: 193 KGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVTLVAEH-KASV 252
           KG+  + I  + WA    LQ   LK+Y   + +LT  +CFLG++++  V L  E    S 
Sbjct: 183 KGASLIAIGCICWAGFINLQAITLKSYP-VELSLTAYICFLGSIESTIVALFIERGNPSA 242

Query: 253 WRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILAE 312
           W I  D  LLAA Y G++ S I YYVQG++MK RGPVF TAF+PL M+IVAI+GS ILAE
Sbjct: 243 WAIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAE 302

Query: 313 KIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVADEIP 353
            +FLG I+G+I+IV GLYSVLWGK K+   S  + +  E+P
Sbjct: 303 VMFLGRILGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKELP 340

BLAST of Spg009623 vs. ExPASy TrEMBL
Match: A0A6J1CEJ4 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111010012 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.2e-191
Identity = 368/418 (88.04%), Postives = 382/418 (91.39%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSLS CN FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLSCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPFVLFFERRGQP+ITLKVF QIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFVLFFERRGQPKITLKVFTQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNN 180
           MTFVMAVIFRME LDMKK+RCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHS  S+ N
Sbjct: 121 MTFVMAVIFRMENLDMKKLRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSSNSSTN 180

Query: 181 NVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240
           +  +++ANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA
Sbjct: 181 S-DENSANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240

Query: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300
           VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII
Sbjct: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300

Query: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVA--DEIPEAIKAN 360
           VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKH+ENLE KSAS A  DEIPEAIKAN
Sbjct: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHRENLEIKSASAAADDEIPEAIKAN 360

Query: 361 NSS--QPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIPRE 415
           ++   QPNNNNN N              PNK    +NSLFI++ DPENPIKPNQ+PRE
Sbjct: 361 SNPNLQPNNNNNGN------------MGPNK---PSNSLFITISDPENPIKPNQVPRE 402

BLAST of Spg009623 vs. ExPASy TrEMBL
Match: A0A6J1J918 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111483690 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.2e-185
Identity = 356/418 (85.17%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLS     SFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSF----SFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           I PFVLFFERRGQP+ITLK+FIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IVPFVLFFERRGQPKITLKIFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNN 180
           MTFVMAVIFRME LDMKK+RCQAK+LGTLVTV GAM+MTLYKGPLVQM WSKHSH S  N
Sbjct: 121 MTFVMAVIFRMENLDMKKLRCQAKVLGTLVTVGGAMVMTLYKGPLVQMPWSKHSHHS--N 180

Query: 181 NVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240
             SD  ANKDWFKGS+FLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCF+GTLQAIA
Sbjct: 181 QASDDKANKDWFKGSMFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFVGTLQAIA 240

Query: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300
           VTLVAEHKA+VWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII
Sbjct: 241 VTLVAEHKAAVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300

Query: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVA----DEIPEAIK 360
           VAIMGSFILAE IFLGGIIGSI IVFGLYSVLWGKH+ENLE K A+ A    +EIP+ IK
Sbjct: 301 VAIMGSFILAENIFLGGIIGSIFIVFGLYSVLWGKHRENLEIKVAAAAASSDEEIPQPIK 360

Query: 361 ANNSSQPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIPRE 415
               SQPNNNNNNNNIA                  NNS+FISMP PENPIKPNQIPRE
Sbjct: 361 L--PSQPNNNNNNNNIA------------------NNSIFISMPAPENPIKPNQIPRE 392

BLAST of Spg009623 vs. ExPASy TrEMBL
Match: A0A1S3BDW4 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103488823 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.2e-183
Identity = 352/415 (84.82%), Postives = 366/415 (88.19%), Query Frame = 0

Query: 1   MGKL--SLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT 60
           MGKL    + CN FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT
Sbjct: 1   MGKLISFFNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT 60

Query: 61  ASIAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML 120
           ASIAPFVLFFERRGQPR+T KVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML
Sbjct: 61  ASIAPFVLFFERRGQPRMTFKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML 120

Query: 121 PAMTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHAST 180
           PAMTFVMAVIFRMEKL+MKK+RCQAK+LGTLVTV GAMLMTLYKGP +QM WSK+SH + 
Sbjct: 121 PAMTFVMAVIFRMEKLEMKKLRCQAKVLGTLVTVGGAMLMTLYKGPPLQMPWSKNSHLT- 180

Query: 181 NNNVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQA 240
             N S+S+ NKDWFKGSIFLIIATLAWASLFVLQNQ LKTYKNHQFTLTTLVCF+GTLQA
Sbjct: 181 --NSSESSNNKDWFKGSIFLIIATLAWASLFVLQNQVLKTYKNHQFTLTTLVCFMGTLQA 240

Query: 241 IAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMM 300
           IAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMM
Sbjct: 241 IAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMM 300

Query: 301 IIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVAD-EIPEAIKA 360
           IIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKS S  D EIPE IKA
Sbjct: 301 IIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSGSDHDNEIPEVIKA 360

Query: 361 NNSSQPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIP 413
           +N+SQPN                        ISNNNS+FISMP PE P KPNQIP
Sbjct: 361 SNNSQPN------------------------ISNNNSIFISMPTPETPTKPNQIP 388

BLAST of Spg009623 vs. ExPASy TrEMBL
Match: A0A6J1HPD7 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111466510 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 9.9e-183
Identity = 351/414 (84.78%), Postives = 374/414 (90.34%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSLS  +SF+ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLSCWSSFVENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPF  FFERRGQP+ITLKVF QIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFAFFFERRGQPKITLKVFTQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNN 180
           MTF+MA+IFRMEKL+MKK+RCQAK LGTLVTV GAMLMTLYKGPL+QMAWSKHSH ST++
Sbjct: 121 MTFMMALIFRMEKLEMKKLRCQAKFLGTLVTVVGAMLMTLYKGPLIQMAWSKHSHVSTSS 180

Query: 181 NVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240
           N   ++ANKDWFKGSIFLIIATLAW+SLFVLQNQALKTY NHQ TLTTLVC +GTLQAIA
Sbjct: 181 N-DQASANKDWFKGSIFLIIATLAWSSLFVLQNQALKTYMNHQLTLTTLVCLMGTLQAIA 240

Query: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300
           VT+VAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII
Sbjct: 241 VTVVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300

Query: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVAD--EIPEAIKAN 360
           VAIMGSFIL+EKI+LGGIIGSILIVFGLYSVLWGKHKENLE+KSA   D  ++P+AIK  
Sbjct: 301 VAIMGSFILSEKIYLGGIIGSILIVFGLYSVLWGKHKENLETKSAVDDDTHKLPQAIKL- 360

Query: 361 NSSQPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIP 413
            SSQPNNNNN     NLE        PNKL+   NSL ISMPDPE+PIKPNQ P
Sbjct: 361 -SSQPNNNNN----LNLE--------PNKLL---NSLIISMPDPESPIKPNQNP 396

BLAST of Spg009623 vs. ExPASy TrEMBL
Match: A0A6J1G2G0 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111450059 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.3e-182
Identity = 352/413 (85.23%), Postives = 373/413 (90.31%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSLS  +SF+ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLSCWSSFVENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPF  FFERR QP+ITLKVF QIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFAFFFERREQPKITLKVFTQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNN 180
           MTFVMAVIFRMEKL+MKK+RCQAK LGTLVTV GAMLMTLYKGPL+QMAWSKHSH  T++
Sbjct: 121 MTFVMAVIFRMEKLEMKKLRCQAKFLGTLVTVVGAMLMTLYKGPLIQMAWSKHSHVPTSS 180

Query: 181 NVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIA 240
           N   ++ANKDWFKGSIFLIIATLAW+SLFVLQNQALKTY NHQ TLTTLVCF+GTLQAIA
Sbjct: 181 N-DQASANKDWFKGSIFLIIATLAWSSLFVLQNQALKTYMNHQLTLTTLVCFMGTLQAIA 240

Query: 241 VTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300
           VT+VAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII
Sbjct: 241 VTVVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMII 300

Query: 301 VAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSA-SVADEIPEAIKANN 360
           VAIMGSFIL+EKI+LGGIIGSILIVFGLYSVLWGKHKENLE+KSA   A ++P+AIK   
Sbjct: 301 VAIMGSFILSEKIYLGGIIGSILIVFGLYSVLWGKHKENLETKSADDDAHKLPQAIKL-- 360

Query: 361 SSQPNNNNNNNNIANLEANEMMIKAPNKLISNNNSLFISMPDPENPIKPNQIP 413
           SSQPNNNNN     NLE        PNKL   +NSL ISMPDPE+PIKPNQ P
Sbjct: 361 SSQPNNNNN----LNLE--------PNKL---SNSLIISMPDPESPIKPNQNP 395

BLAST of Spg009623 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 504.6 bits (1298), Expect = 7.6e-143
Identity = 259/385 (67.27%), Postives = 315/385 (81.82%), Query Frame = 0

Query: 1   MGKLSLSGCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           M    +S C SFL +SKPYFAMISLQFGYAGMNII+K+SL+ GMSHYVLVVYRHA ATA 
Sbjct: 1   MSMEEISSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAV 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPF  FFER+ QP+IT  +F+Q+F+L LLGPVIDQNFYY GLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPA 120

Query: 121 MTFVMAVIFRMEKLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKH------S 180
           MTF++AV+FRME LD+KK+ CQAKI GT+VTVAGAMLMT+YKGP+V++ W+K+      S
Sbjct: 121 MTFILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSS 180

Query: 181 HASTNNNVSDSNANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLG 240
           HA+T ++  +S+++K++ KGSI LI ATLAWASLFVLQ + LKTY  HQ +LTTL+CF+G
Sbjct: 181 HANTTSS-KNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIG 240

Query: 241 TLQAIAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFS 300
           TLQA+AVT V EH  S WRIGWDMNLLAAAY+GIV SSISYYVQG+VMKKRGPVFATAFS
Sbjct: 241 TLQAVAVTFVMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFS 300

Query: 301 PLMMIIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKEN----LESKSASVADE 360
           PLMM+IVA+MGSF+LAEKIFLGG+IG++LIV GLY+VLWGK KEN     E        +
Sbjct: 301 PLMMVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSK 360

Query: 361 IPEAIKANNSSQPNNNNNNNNIANL 376
           + E ++AN S    +  +N+ ++ +
Sbjct: 361 VTEDVEANGSKMKISEGDNSMLSTI 384

BLAST of Spg009623 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 374.0 bits (959), Expect = 1.5e-103
Identity = 184/326 (56.44%), Postives = 257/326 (78.83%), Query Frame = 0

Query: 14  ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRGQ 73
           E++KPYFAM+ LQFGYAGMN+++K  L RGMSHYVLV YR+AFATA+IAPF L  ER+ +
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 74  PRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRMEK 133
            ++T  +F++IF+LALLGPVIDQN YY GLK TSPTFS A+SN++PA+T ++A +FRMEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 134 LDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNNNVSDSNANKDWFK 193
           ++M+KVRC  K++GTLVTV G++LM  YKGP +    S  + AS       S    D+ K
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFFRSHLTAAS-------SPPTADYLK 186

Query: 194 GSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVTLVAEHKASVWR 253
            ++FL++A+L+WAS FVLQ   LK Y  H  +++T+VCF+GTLQ++A+  V EH  S   
Sbjct: 187 AAVFLLLASLSWASFFVLQAATLKKYSAH-LSMSTMVCFMGTLQSLALAFVMEHNPSALN 246

Query: 254 IGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILAEKI 313
           IG+DMNLLA+AYAGI++SSI+YYVQGL+M+++GPVF TAF+PL+++IV+IM  F+L + I
Sbjct: 247 IGFDMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGI 306

Query: 314 FLGGIIGSILIVFGLYSVLWGKHKEN 340
           +LGG+IG ++++ G+Y+VLWGKH ++
Sbjct: 307 YLGGVIGVVVLMVGVYAVLWGKHVDD 324

BLAST of Spg009623 vs. TAIR 10
Match: AT3G56620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.4e-99
Identity = 187/339 (55.16%), Postives = 262/339 (77.29%), Query Frame = 0

Query: 14  ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRGQ 73
           E++KPYFAM+ LQFGYAGMN+++KV L RGMSHYVLV YR+AFATA+IAPF L  ER+ +
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 74  PRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRMEK 133
           P++T  +F+QIFVLALLGP+IDQN YYAGLK TSPTF+ A++N++PA+TF++++I RMEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 134 LDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNNNVSDSNANKDWFK 193
           ++M+KVR QAK++GTLV V GAMLM L+K PL+       SH + +   + S A +D+ K
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFL---RSHLTGH---ALSPAGEDYLK 186

Query: 194 GSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVTLVAEHKASVWR 253
            ++FL+IA+ +WAS FVLQ   LK Y +H  +L+T+VCF+GTLQ+ A+T V E   S W 
Sbjct: 187 ATVFLLIASFSWASFFVLQAATLKRYSSH-LSLSTMVCFMGTLQSTALTFVMEPNLSAWN 246

Query: 254 IGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILAEKI 313
           IG+DMNLLA+AYAGI++SSI+YYVQG++ K++  +F TAF+PL++II +I+G  IL + +
Sbjct: 247 IGFDMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLILNQTL 306

Query: 314 FLGGIIGSILIVFGLYSVLWGK-----HKENLESKSASV 348
            LGG++G  ++V G+ +VLWGK      +EN+E K   +
Sbjct: 307 NLGGVLGMAILVVGVCTVLWGKEGDIDEEENIEEKFVEI 338

BLAST of Spg009623 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 336.3 bits (861), Expect = 3.6e-92
Identity = 166/333 (49.85%), Postives = 236/333 (70.87%), Query Frame = 0

Query: 13  LENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRG 72
           + + KPY AMIS+QFGYAGM II+ VSL  GM+HYVL VYRHA ATA IAPF LF ER+ 
Sbjct: 6   MNSLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERKI 65

Query: 73  QPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRME 132
           +P++T ++F+QI +L  + PV+DQN YY G+ YTS TF+ A +N+LPA+TFV+A+IFR+E
Sbjct: 66  RPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRLE 125

Query: 133 KLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQM-------AWSKHSHASTNNNVSDS 192
            ++ KKVR  AK++GT++TV+GA+LMTLYKGP+V                  ++     +
Sbjct: 126 SVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAA 185

Query: 193 NANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVTLVA 252
             +K W  G++ L+  T  WA  F+LQ+  LK Y   + +LTTL+C +GTL+  AV+LV 
Sbjct: 186 AMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYP-AELSLTTLICLMGTLEGTAVSLVT 245

Query: 253 EHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMG 312
               S W+IG+D NL AAAY+G++ S ++YYVQG+VM++RGPVF   F+PL ++I A +G
Sbjct: 246 VRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITAALG 305

Query: 313 SFILAEKIFLGGIIGSILIVFGLYSVLWGKHKE 339
             +L+E I LG +IG++ I+ GLY+V+WGK K+
Sbjct: 306 VVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKD 337

BLAST of Spg009623 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.3e-89
Identity = 172/341 (50.44%), Postives = 238/341 (69.79%), Query Frame = 0

Query: 13  LENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRG 72
           L+  KP+  ++SLQFGYAG++II+K +L++GMS +VL  YRH  AT  IAPF  F +R+ 
Sbjct: 3   LKTWKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKI 62

Query: 73  QPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMTFVMAVIFRME 132
           +P++TL +F +I +L LL P IDQN YY G+KYTS TF+ AM+N+LPA  F+MA IFR+E
Sbjct: 63  RPKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLE 122

Query: 133 KLDMKKVRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSHASTNNNVSDSNANKDWF 192
           K+++KK+  QAKILGT+VTV GAMLMT+ KGPL+ + W+  +    + + S++   +D  
Sbjct: 123 KVNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWA--NPHDIHQDSSNTGVKQDLT 182

Query: 193 KGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVTLVAEH-KASV 252
           KG+  + I  + WA    LQ   LK+Y   + +LT  +CFLG++++  V L  E    S 
Sbjct: 183 KGASLIAIGCICWAGFINLQAITLKSYP-VELSLTAYICFLGSIESTIVALFIERGNPSA 242

Query: 253 WRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILAE 312
           W I  D  LLAA Y G++ S I YYVQG++MK RGPVF TAF+PL M+IVAI+GS ILAE
Sbjct: 243 WAIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAE 302

Query: 313 KIFLGGIIGSILIVFGLYSVLWGKHKENLESKSASVADEIP 353
            +FLG I+G+I+IV GLYSVLWGK K+   S  + +  E+P
Sbjct: 303 VMFLGRILGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKELP 340