Spg009304 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg009304
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	NADH-ubiquinone oxidoreductase chain 4
Location	scaffold32: 191457 .. 195777 (-)
RNA-Seq Expression	Spg009304
Synteny	Spg009304

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGCAACAAAAGCTTGATTCGAGGCGTCAGCAAAACACTGCCGTCTGTTCCAAAAACAAAAGCCCCTTAGCGCCCCGGGACGGGAGTGGGGGACGGCCCTACGCGCAGGCAACAGCAGCACCAGCTCTACGAAGTCAGAATCGAATCTTTCTGTTGGCTTTCCCAATTCATTCGTGAATCAGAATGAAAGGGCTAGAAGCTCTAGCTTCTAGCTACTTCGCCTGCTTATTATTATGGCGGAAATGAACGTGCTATTTTCAAATCGATTGATAGATAGCCTGATCTGTTCTGATAGATCGAAAGAGTCGAAATGTCTCGAGAGGGGATCCATCAATAATAAGGAGAAATCTCCAATTTCTATCTATTCTTGATCCATCAGAAAGAAATCGATATGTATCTGATGGAGTCTACATCGTACGTAGAGCGCCCAAGCGCTTTTTAGGCCTGCTCCGTTCTCTTATCCATCGGTCCAATGCACTGGGCTCATCTCGTGGAGGGAAAAGCTAAAATTGAGTTGTAGTCTTGTTGTTCGCCGCCTCGACGCATTCCCTTCTCTCCCCGGCATCGTCCCACACAGAAAGAAAGAGCGCAGAGCCCCGGCCCGACCTGTGGGTCCGCTAACGTAAAGCGAGGAGTTGAGCCTGAACTGGCGAACCGAAGTCACTTTCGGAACCATACTTCCTACAGCTAACACGTGTCCAGCGGGAACGCGCAGCGCAACCGAACGTGAGCTGCTATACCGAATCCCCCGCAGGCCATCGGGGACCGAGTGGTAAGGCCATGATCCGCAGGGGAACGGATCACTCATTCTTCCATTGGGGACAGGTGCACGAACGACAACTCCAAACGTCACACATCCGTCGCCTACCTACCGTTTAGGTGGCACCAGCGAGATCCAGCCCACGGAGCGGTAAGGAACTTCGTGTCGCGGCTGCTCCACTCCGCCGCGGTCTCATGAACTGAACTTCGTTGCCTTCCTGCGCGCGAAGCGAATTGGCGCTGTGCTGTGGGTCAGTTTCGGGGCGGGGAGCGAAGAGAGACCAGAAGAATGATTCATTTGGATCGACGAGCTTTTTCAGCCCCAAAACTCAGAATCAATGGAATGAATGTATCTAAGGCTATCCATGAAAAGATCGATTCTATCTGTATAGATAGGGGATCTCTCTATACATATAAAATCGTTTTATGGCATGATATGATCGCCTAGCCGTAGCCGCATATCAGCTGCGCTCAATATGCGGGATTTCCGTTGGATCTGATCTTTCGGGAAGCTTTTGGACCTAGCGAAAAGGACTTTCAGCCTTTGCGCAAGCAAGCAAAGTCGAAGCTTTGCCAGAAGCAAGACGAGGAAGCGGAGCTGTCGTATAAACGGTAGCTTCCCCCGACCTACTTTGATTCAAAAGAAAGGCGAAGGGTTTTTTGGCAACAAGCAAACGACTTTCTATCATAGTTGCAAGGGTTCCAAACCTTAACTACAACATGTTGTACCAAAGGCTTCTTTCGGTTGGTTGAATAAGGGGGCCGTTCAATCTAAGACAGCGCTGTCTTAGATTGAACGGCAATAAGATAAGTCGACGTTCAATCTCTAAGGCGGGTTTCCGCTGAGAACGGAATAGTGTTCGTGTCCAAAGGTGAATAAAGCCCTAGCTTCTAGAGTTCGCTGCTTTTCCCAGGCCGGAGAAGGGCTTATACTGATCGCCTTTGTTTGATATGTTCATTCAGTACCAATACAAACTACAACTACACTAAAGTGGAAGGGCTACTATAGAAGCTAGAAGTAGCCCCTCGTAAAGTAAGTAGTCGGCCTAATCAAAGCGTCACGACAACAAAAAATTACCCTGATGAATCGAATGGAATCTTAATTCAATAGGGGGCCGCCTACCAATCAAAGAGGCTGGAAGACTTTCCACTTCATTGAAAAAGGGAAGGGGAGAGGGAAGCGAAGCTTAGCGAGCTTTCTGCCGGCCGACCACTACATAAGCCGCTGGCGTCGAGCTTTCTCCGCCAGCTTCCTTTCATTCGCTTCGCGGGAGCCGCACAGCACATAGCTGGAAGTCAGAGGGCCCATACTACCTGCCTAACCCCTCGCTCCGAGGGACCGTAGATCGGAAAGCGCCTTCTTAATCACCCCATTCATCCAAAAGAGAAGGGGCCTATGTATTTGCATGACCCCTGCAGATTTGACTCTATCTACACTCGGAGCCAATCCCCTATCGGTCCTGCCACCACGCCGCAGAACGGGAGCTCGTGTGGAACCTTTTCTTTCTGGCGTAACTGCGGTGAAACGTAACAAAATCTTACGGTCGCGTAATAGAAGGGCCGGAGGTACGGTAAACTCGGCCAAAATATGACACCCGAAGGGCCCGGCGCGCACAATCCTATCCTATCTGAGTCCGAGTTTACCCTTGCACTTCGGACAGCCGTCCGTAGCATCATAAGGAGGACCCCCTTTCGAGGTCAAAAAATGGTACGGTACATAGGAGGCTGGTCTTTCTCAACGTGGTGTATAGCACGAAAAACCTTTCGATACAAGATAGGGCCGTTCACATGAAAGAAGAGAAGAAAGACTTTCTTCTCTTCTTTCTCTTTTTCGAGGGTCTTATGGGGGGAGGGGGGACATTCGGGCGCATTTCTCAAAATCCTCCACTGCATCCAGGATCACAAGTGGAACGCTAAGCAGGGGTGGGAAGGCTGCGAGCTCCGCAACAAAAGCGAAGCGAGCCCCTGACTCACCTCTCTCTAGAAAGCCCTATTAGTCAAGCTTGAAAGCCGTTGCGCGCTAGTAGCGCACATCCGTTTTTCAGCTGGCTTCAAAGTGCTAGTTGTAGCGCTCCCGCGCCCTTTGTATATAAAGGTAAGGCTCTTCTGTGGAGTCGCACTCCACGAGTCTTGTCTTCCCTCCAACGACTAGCTTCCTTCCCTTTCTTGTTCCGGTCCGACAACGAGAACGCTGCCCTGTCCTTCTCCTGCCGTGGAAGGACTTCAGCCTGTGGTGTGGTCCAACCCATGGGCGGAGTCGCCCTCATCCCCCCCATTGCTCAGTGCTCCCTGCTGCTTCGCAGGGCTTTACCCGTCCCCGGCGTGGACGCGGGCTTCTATAAGAGAATGAGAAGCTCTCAACACAAGAAAACGCGAACCGCGCGGAGCCCACCCGAGTTCGTTTTCCGCCGCCACAGGCCGGCCGCTGGGGAAACACAGCCCGGCCCCCTCTCGGGAAACGGGGGTCCAACAAGCACTTGCTGCAGAGGGGGCCCACAAGCACCCGGCCCGCCCCTTCCTTCTTCTTCAGTAAAGCCGACCTCTTTTTCGCCAGTGCTGACGCAGTGCGCACGTAGATAAGGAAAGCCAAATCCCAGTGGGGGGCCGGTGCCTTTCTTTTACGGCCTAAATTCCTATTTGTCAGCCGTGGGGAACTACTGCTCGGTCGGGAAGAGGGGAAAGGCTTGGGCCTACCTATCCCGATAGGACCTCATAAAGGAACGGGAGCTGTTGAGAGGTTCCATATTGCCGAGCCGAAGGGCAGCACTTCTGTACGTGATCGTAGTATGTCACGTCGTCTCGTCCCCGCAGCATTGAAGAGTATCTATGCACTATTTCCGGTTCACTGATAAGGAGGATAGAGTTGGGGTGGGGGTGACGGATGTGATACGTAAAGTATGACCCGGAGAGATACATGCTAACTATGGGTAGAAAGCAGGAACCATTATGTAAAGAATTTCGGGGGGTTACAGATCTCTTATACTACCATCGATCGACAGAGCGGAACAACCAGAAAAAGAAGTGAAGTTAGAAAGCCGTATGATAGGTGGTAACTATCTTGTACGGTTCGGGGGGTAATCGGCGTACTCGTATCAGTGGGGGGGAATCTTTGGCTCTATCGAACATACAGGGAATTGGAGGTAGCATTCTACCGATGTTAAGTCATGGACTGGTTCCTTCAGCCCTTTTTCTATGTGTTGGTGTTCTATATGACCGACATCAGACTCGACTTGTTAGATATTACGGAGGTTCAGTGAGCACCATGCCGAATCTCTCTACCATTTCCTTCTCTTTCACTTTGGCCAATATGAGTTTACCTGGTACTAGCAGCTTTATCGGGGAATTTCCCATCTCAGTAGGAGCTTTCCAAAGAAATAGCTTAGTAGCCACATTAGCCGCGCTTGGGATGATTTTAGGCGCGGCGTATTCCCTTTGGCTATATAATCGTGTGGTTTCTGGAAATTTAAAACCCGATTTCCTCCATAAATTCTCCGATCCAAATGGCAGAGAAGTTTCCATATTTATACCTTTTCTTGTTGGAGGGGCGACCGCCCGTTGA

mRNA sequence

ATGTCGCAACAAAAGCTTGATTCGAGGCGTCAGCAAAACACTGCCGTCTGTTCCAAAAACAAAAGCCCCTTAGCGCCCCGGGACGGGAGTGGGGGACGGCCCTACGCGCAGGCAACAGCAGCACCAGCTCTACGAAGTCAGAATCGAATCTTTCTGTTGGCTTTCCCAATTCATTCAAATCGATATGTATCTGATGGAGTCTACATCCTAACACGTGTCCAGCGGGAACGCGCAGCGCAACCGAACGTGAGCTGCTATACCGAATCCCCCGCAGGCCATCGGGGACCGAGTGGTAAGGCCATGATCCGCAGGGGAACGGATCACTCATTCTTCCATTGGGGACAGCCGCATATCAGCTGCGCTCAATATGCGGGATTTCCGTTGGATCTGATCTTTCGGGAAGCTTTTGGACCTAGCGAAAAGGACTTTCAGCCTTTGCGCAAGCAAGCAAAGTCGAAGCTTTGCCAGAAGCAAGACGAGGAAGCGGAGCTGTCGTATAAACGGGAAGGGGAGAGGGAAGCGAAGCTTAGCGAGCTTTCTGCCGGCCGACCACTACATAAGCCGCTGGCGTCGAGCTTTCTCCGCCAGCTTCCTTTCATTCGCTTCGCGGGAGCCGCACAGCACATAGCTGGAAATTTGACTCTATCTACACTCGGAGCCAATCCCCTATCGGTCCTGCCACCACGCCGCAGAACGGGAGCTCGTGTGGAACCTTTTCTTTCTGGCGTAACTGCGGTGAAACGTAACAAAATCTTACGGTCGCGTAATAGAAGGGCCGGAGGTAAGGCTCTTCTGTGGAGTCGCACTCCACGAGTCTTGTCTTCCCTCCAACGACTAGCTTCCTTCCCTTTCTTGTTCCGGTCCGACAACGAGAACGCTGCCCTGTCCTTCTCCTGCCGTGGAAGGACTTCAGCCTGTGGTGTGGTCCAACCCATGGGCGGAGTCGCCCTCATCCCCCCCATTGCTCAGTGCTCCCTGCTGCTTCGCAGGGCTTTACCCGTCCCCGGCGTGGACGCGGGCTTCTATAAGAGAATGAGAAGCTCTCAACACAAGAAAACGCGAACCGCGCGGAGCCCACCCGAGTTCGTTTTCCGCCGCCACAGGCCGGCCGCTGGGGAAACACAGCCCGGCCCCCTCTCGGGAAACGGGGGTCCAACAAGCACTTGCTGCAGAGGGGGCCCACAAGCACCCGGCCCGCCCCTTCCTTCTTCTTCAGTAAAGCCGACCTCTTTTTCGCCAGTGCTGACGCACCGTGGGGAACTACTGCTCGGTCGGGAAGAGGGGAAAGGCTTGGGCCTACCTATCCCGATAGGACCTCATAAAGGAACGGGAGCTGTTGAGAGGTTCCATATTGCCGAGCCGAAGGGCAGCACTTCTGGAATTGGAGGTAGCATTCTACCGATGTTAAGTCATGGACTGGTTCCTTCAGCCCTTTTTCTATGTGTTGGTGTTCTATATGACCGACATCAGACTCGACTTGTTAGATATTACGGAGGTTCAGTGAGCACCATGCCGAATCTCTCTACCATTTCCTTCTCTTTCACTTTGGCCAATATGAGTTTACCTGGTACTAGCAGCTTTATCGGGGAATTTCCCATCTCAGTAGGAGCTTTCCAAAGAAATAGCTTAGTAGCCACATTAGCCGCGCTTGGGATGATTTTAGGCGCGGCGTATTCCCTTTGGCTATATAATCGTGTGGTTTCTGGAAATTTAAAACCCGATTTCCTCCATAAATTCTCCGATCCAAATGGCAGAGAAGTTTCCATATTTATACCTTTTCTTGTTGGAGGGGCGACCGCCCGTTGA

Coding sequence (CDS)

ATGTCGCAACAAAAGCTTGATTCGAGGCGTCAGCAAAACACTGCCGTCTGTTCCAAAAACAAAAGCCCCTTAGCGCCCCGGGACGGGAGTGGGGGACGGCCCTACGCGCAGGCAACAGCAGCACCAGCTCTACGAAGTCAGAATCGAATCTTTCTGTTGGCTTTCCCAATTCATTCAAATCGATATGTATCTGATGGAGTCTACATCCTAACACGTGTCCAGCGGGAACGCGCAGCGCAACCGAACGTGAGCTGCTATACCGAATCCCCCGCAGGCCATCGGGGACCGAGTGGTAAGGCCATGATCCGCAGGGGAACGGATCACTCATTCTTCCATTGGGGACAGCCGCATATCAGCTGCGCTCAATATGCGGGATTTCCGTTGGATCTGATCTTTCGGGAAGCTTTTGGACCTAGCGAAAAGGACTTTCAGCCTTTGCGCAAGCAAGCAAAGTCGAAGCTTTGCCAGAAGCAAGACGAGGAAGCGGAGCTGTCGTATAAACGGGAAGGGGAGAGGGAAGCGAAGCTTAGCGAGCTTTCTGCCGGCCGACCACTACATAAGCCGCTGGCGTCGAGCTTTCTCCGCCAGCTTCCTTTCATTCGCTTCGCGGGAGCCGCACAGCACATAGCTGGAAATTTGACTCTATCTACACTCGGAGCCAATCCCCTATCGGTCCTGCCACCACGCCGCAGAACGGGAGCTCGTGTGGAACCTTTTCTTTCTGGCGTAACTGCGGTGAAACGTAACAAAATCTTACGGTCGCGTAATAGAAGGGCCGGAGGTAAGGCTCTTCTGTGGAGTCGCACTCCACGAGTCTTGTCTTCCCTCCAACGACTAGCTTCCTTCCCTTTCTTGTTCCGGTCCGACAACGAGAACGCTGCCCTGTCCTTCTCCTGCCGTGGAAGGACTTCAGCCTGTGGTGTGGTCCAACCCATGGGCGGAGTCGCCCTCATCCCCCCCATTGCTCAGTGCTCCCTGCTGCTTCGCAGGGCTTTACCCGTCCCCGGCGTGGACGCGGGCTTCTATAAGAGAATGAGAAGCTCTCAACACAAGAAAACGCGAACCGCGCGGAGCCCACCCGAGTTCGTTTTCCGCCGCCACAGGCCGGCCGCTGGGGAAACACAGCCCGGCCCCCTCTCGGGAAACGGGGGTCCAACAAGCACTTGCTGCAGAGGGGGCCCACAAGCACCCGGCCCGCCCCTTCCTTCTTCTTCAGTAAAGCCGACCTCTTTTTCGCCAGTGCTGACGCACCGTGGGGAACTACTGCTCGGTCGGGAAGAGGGGAAAGGCTTGGGCCTACCTATCCCGATAGGACCTCATAAAGGAACGGGAGCTGTTGAGAGGTTCCATATTGCCGAGCCGAAGGGCAGCACTTCTGGAATTGGAGGTAGCATTCTACCGATGTTAAGTCATGGACTGGTTCCTTCAGCCCTTTTTCTATGTGTTGGTGTTCTATATGACCGACATCAGACTCGACTTGTTAGATATTACGGAGGTTCAGTGAGCACCATGCCGAATCTCTCTACCATTTCCTTCTCTTTCACTTTGGCCAATATGAGTTTACCTGGTACTAGCAGCTTTATCGGGGAATTTCCCATCTCAGTAGGAGCTTTCCAAAGAAATAGCTTAGTAGCCACATTAGCCGCGCTTGGGATGATTTTAGGCGCGGCGTATTCCCTTTGGCTATATAATCGTGTGGTTTCTGGAAATTTAAAACCCGATTTCCTCCATAAATTCTCCGATCCAAATGGCAGAGAAGTTTCCATATTTATACCTTTTCTTGTTGGAGGGGCGACCGCCCGTTGA

Protein sequence

MSQQKLDSRRQQNTAVCSKNKSPLAPRDGSGGRPYAQATAAPALRSQNRIFLLAFPIHSNRYVSDGVYILTRVQRERAAQPNVSCYTESPAGHRGPSGKAMIRRGTDHSFFHWGQPHISCAQYAGFPLDLIFREAFGPSEKDFQPLRKQAKSKLCQKQDEEAELSYKREGEREAKLSELSAGRPLHKPLASSFLRQLPFIRFAGAAQHIAGNLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTAVKRNKILRSRNRRAGGKALLWSRTPRVLSSLQRLASFPFLFRSDNENAALSFSCRGRTSACGVVQPMGGVALIPPIAQCSLLLRRALPVPGVDAGFYKRMRSSQHKKTRTARSPPEFVFRRHRPAAGETQPGPLSGNGGPTSTCCRGGPQAPGPPLPSSSVKPTSFSPVLTHRGELLLGREEGKGLGLPIPIGPHKGTGAVERFHIAEPKGSTSGIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDPNGREVSIFIPFLVGGATAR

Homology

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BLAST of Spg009304 vs. NCBI nr
Match: KAA0052380.1 (NADH-quinone oxidoreductase protein [Cucumis melo var. makuwa])

HSP 1 Score: 344.0 bits (881), Expect = 2.7e-90
Identity = 220/423 (52.01%), Postives = 220/423 (52.01%), Query Frame = 0


Query: 188 PLASSFLRQLPFIRFAGAAQHIAGNLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTAVK 247
           P   SF   LPFIRFAGAAQHIAGNLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTAVK
Sbjct: 17  PWGGSF--SLPFIRFAGAAQHIAGNLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTAVK 76

Query: 248 RNKILRSRNRRAGGKALLWSRTPRVLSSLQRLASFPFLFRSDNENAALSFSCRGRTSACG 307
                      A GK                                             
Sbjct: 77  -----------AVGK--------------------------------------------- 136

Query: 308 VVQPMGGVALIPPIAQCSLLLRRALPVPGVDAGFYKRMRSSQHKKTRTARSPPEFVFRRH 367
                                                                       
Sbjct: 137 ------------------------------------------------------------ 196

Query: 368 RPAAGETQPGPLSGNGGPTSTCCRGGPQAPGPPLPSSSVKPTSFSPVLTHRGELLLGREE 427
                                                                  LGREE
Sbjct: 197 -------------------------------------------------------LGREE 256

Query: 428 GKGLGLPIPIGPHKGTGAVERFHIAEPKGSTS-----------------------GIGGS 487
           GKGLGLPIPIGPHKG GAVERFHIAEPKGSTS                       GIGGS
Sbjct: 257 GKGLGLPIPIGPHKGRGAVERFHIAEPKGSTSLRDRSISPPLVPAAFHSIYALFPGIGGS 266

Query: 488 ILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGT 547
           ILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGT
Sbjct: 317 ILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGT 266

Query: 548 SSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDPNG 588
           SSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGN KPDFLHKFSDPNG
Sbjct: 377 SSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNCKPDFLHKFSDPNG 266

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BLAST of Spg009304 vs. NCBI nr
Match: PRQ15629.1 (putative NADH:ubiquinone reductase (H(+)-translocating) [Rosa chinensis])

HSP 1 Score: 331.6 bits (849), Expect = 1.4e-86
Identity = 170/188 (90.43%), Postives = 173/188 (92.02%), Query Frame = 0


Query: 422 LLGREEGKGLGLPIPIGPHKGTGAVERFHIAEPKGSTS----------GIGGSILPMLSH 481
           +L RE GKGLGLPIPIGPHKGTGAVERFHIAEPKGSTS          GIGGSILPMLSH
Sbjct: 45  VLWREGGKGLGLPIPIGPHKGTGAVERFHIAEPKGSTSIRDRSMSRRLGIGGSILPMLSH 104

Query: 482 GLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGTSSFIGEF 541
           GLVPSALFLCVGVLYDRH+TRLVRYYGGSVSTMPNLSTISFS TLANMS PGTSSFIGEF
Sbjct: 105 GLVPSALFLCVGVLYDRHKTRLVRYYGGSVSTMPNLSTISFSSTLANMSSPGTSSFIGEF 164

Query: 542 PISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDPNGREVSIFI 600
           PI VGAFQRNSLVATLAALGMILGAAYSLWLYNR+VSGNLKPDFLHKFSDPNGREVSIFI
Sbjct: 165 PILVGAFQRNSLVATLAALGMILGAAYSLWLYNRLVSGNLKPDFLHKFSDPNGREVSIFI 224

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BLAST of Spg009304 vs. NCBI nr
Match: KEH16847.1 (NADH-quinone oxidoreductase protein [Medicago truncatula])

HSP 1 Score: 305.8 bits (782), Expect = 8.0e-79
Identity = 160/186 (86.02%), Postives = 162/186 (87.10%), Query Frame = 0


Query: 430 GLGLPIPIGPHKGTGAVERFHIAEPKGSTSGIGG--------------SILPMLSHGLVP 489
           GLGLPIPIGPHKGTGAV+RFHIAEPKGSTS                  SI PMLSHGLVP
Sbjct: 8   GLGLPIPIGPHKGTGAVDRFHIAEPKGSTSVRDRSMSSRPRCIEKYLCSIPPMLSHGLVP 67

Query: 490 SALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGTSSFIGEFPISV 549
           SALFLCVGVLYDRH+TRLVRYYGG VSTMPNLSTISFS TLANMS PGTSSFIGEFPISV
Sbjct: 68  SALFLCVGVLYDRHKTRLVRYYGGLVSTMPNLSTISFSSTLANMSSPGTSSFIGEFPISV 127

Query: 550 GAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDPNGREVSIFIPFLV 602
           GAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLK DFLHKFSDPNGREVSIFIPFLV
Sbjct: 128 GAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKADFLHKFSDPNGREVSIFIPFLV 187

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BLAST of Spg009304 vs. NCBI nr
Match: KAG6585907.1 (NADH-ubiquinone oxidoreductase chain 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 293.5 bits (750), Expect = 4.1e-75
Identity = 209/476 (43.91%), Postives = 216/476 (45.38%), Query Frame = 0


Query: 127 PLDLIFREAFGPSEKDFQPLRKQAKSKLCQKQDEEAELSYKREGEREAKLS-ELSAGRPL 186
           PLDLIFREAFGPSEKDFQPLRKQAKS            S+ R   R+       S  RP 
Sbjct: 7   PLDLIFREAFGPSEKDFQPLRKQAKS----------NRSFARSKTRKQSCRINASFPRPT 66

Query: 187 HKPLASSFLRQLPFIRFAGAAQHIAGNLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTA 246
           +  +         F        H   +LTLSTLGANPLSVLPPRRRTGARVEPFLSGVTA
Sbjct: 67  YTLIQKK--GSGVFGNKQTTFYHSCKDLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTA 126

Query: 247 VKRNKILRSRNRRAGGKALLWSRTPRVLSSLQRLASFPFLFRSDNENAALSFSCRGRTSA 306
           VK N                                                        
Sbjct: 127 VKDN-------------------------------------------------------- 186

Query: 307 CGVVQPMGGVALIPPIAQCSLLLRRALPVPGVDAGFYKRMRSSQHKKTRTARSPPEFVFR 366
                                                                       
Sbjct: 187 ------------------------------------------------------------ 246

Query: 367 RHRPAAGETQPGPLSGNGGPTSTCCRGGPQAPGPPLPSSSVKPTSFSPVLTHRGELLLGR 426
                                                                       
Sbjct: 247 ------------------------------------------------------------ 269

Query: 427 EEGKGLGLPIPIGPHKGTGAVERFHIAEPKGSTSGIGGSILPMLSHGLVPSALFLCVGVL 486
                                    I +  G   GIGGSIL MLSHGLVPSALFLCVGVL
Sbjct: 307 -------------------------ITKSDGRREGIGGSILLMLSHGLVPSALFLCVGVL 269

Query: 487 YDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGTSSFIGEFPISVGAFQRNSLVA 546
           YDRH+TRLVRYYGG VSTMPNLSTISFSFTLANMSLPGTSSFIGEFPISVGAFQRNSLVA
Sbjct: 367 YDRHKTRLVRYYGGLVSTMPNLSTISFSFTLANMSLPGTSSFIGEFPISVGAFQRNSLVA 269

Query: 547 TLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDPNGREVSIFIPFLVGGATAR 602
           TLAALGMILGAAYSLWLYNRVVSGN K DFLHKFSDPNGREVSIFIPFLVGGATAR
Sbjct: 427 TLAALGMILGAAYSLWLYNRVVSGNFKTDFLHKFSDPNGREVSIFIPFLVGGATAR 269

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BLAST of Spg009304 vs. NCBI nr
Match: TKS02690.1 (NADH-ubiquinone oxidoreductase chain 4 (mitochondrion) [Populus alba])

HSP 1 Score: 283.9 bits (725), Expect = 3.3e-72
Identity = 160/217 (73.73%), Postives = 166/217 (76.50%), Query Frame = 0


Query: 396 APGPPLPSSSVKPTSFSPVLTHRGELLLGRE-EGKGLG--LPIPIGPHKGTGAVERFHIA 455
           A G P     +K       LT +      R  EGKG+G   P PIGPHKG+   ERFH A
Sbjct: 247 AKGAPPARLPIKEAESKRKLTRKPWGTTARSGEGKGVGPTYPDPIGPHKGSH--ERFHRA 306

Query: 456 E--------PKGSTSGIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTM 515
           E           +  GIGGSILPMLSHGLVPSALFLCVGVLYDRH+TRLVRYYGG VSTM
Sbjct: 307 EGLLGGESLALSNIQGIGGSILPMLSHGLVPSALFLCVGVLYDRHKTRLVRYYGGLVSTM 366

Query: 516 PNLSTISFSFTLANMSLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYN 575
           PNLSTI FS TLANMS PGTSSFIGEF ISVGAFQRNSLVATLAALGMILGAAYSLWLYN
Sbjct: 367 PNLSTIFFSSTLANMSSPGTSSFIGEFLISVGAFQRNSLVATLAALGMILGAAYSLWLYN 426

Query: 576 RVVSGNLKPDFLHKFSDPNGREVSIFIPFLVGGATAR 602
           RVVSGNLKPDFLHKFSD NGREVSIFIPFLVGGAT R
Sbjct: 427 RVVSGNLKPDFLHKFSDSNGREVSIFIPFLVGGATVR 461

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BLAST of Spg009304 vs. ExPASy Swiss-Prot
Match: Q04050 (NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris OX=3711 GN=ND4 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.1e-67
Identity = 131/137 (95.62%), Postives = 132/137 (96.35%), Query Frame = 0


Query: 460 GIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANM 519
           GIGGSILPMLSHGLVPSALFLCVGVLYDRH+TRLVRYYGG VSTMPNLSTI FSFTLANM
Sbjct: 330 GIGGSILPMLSHGLVPSALFLCVGVLYDRHKTRLVRYYGGLVSTMPNLSTIFFSFTLANM 389

Query: 520 SLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 579
           S PGTSSFIGEFPI VGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF
Sbjct: 390 SSPGTSSFIGEFPILVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 449

Query: 580 SDPNGREVSIFIPFLVG 597
           SD NGREVSIFIPFLVG
Sbjct: 450 SDSNGREVSIFIPFLVG 466

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BLAST of Spg009304 vs. ExPASy Swiss-Prot
Match: P93313 (NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana OX=3702 GN=ND4 PE=1 SV=2)

HSP 1 Score: 246.1 bits (627), Expect = 9.9e-64
Identity = 127/137 (92.70%), Postives = 128/137 (93.43%), Query Frame = 0


Query: 460 GIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANM 519
           GIGGSIL MLSHGLV SALFLCVGVLYDRH+TRLVRYYGG VSTMPN STI FSFTLANM
Sbjct: 330 GIGGSILLMLSHGLVSSALFLCVGVLYDRHKTRLVRYYGGLVSTMPNFSTIFFSFTLANM 389

Query: 520 SLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 579
           SLPGTSSFIGEF I VGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF
Sbjct: 390 SLPGTSSFIGEFLILVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 449

Query: 580 SDPNGREVSIFIPFLVG 597
           SD NGREV IFIPFLVG
Sbjct: 450 SDLNGREVFIFIPFLVG 466

[Close]

BLAST of Spg009304 vs. ExPASy Swiss-Prot
Match: P27572 (NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum OX=4565 GN=ND4 PE=2 SV=2)

HSP 1 Score: 238.8 bits (608), Expect = 1.6e-61
Identity = 123/137 (89.78%), Postives = 126/137 (91.97%), Query Frame = 0


Query: 460 GIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANM 519
           GIGGSIL MLSHGLV SALFLCVGVLYDRH+TRLVRYYGG VSTMPN STI F FTLANM
Sbjct: 330 GIGGSILLMLSHGLVSSALFLCVGVLYDRHKTRLVRYYGGLVSTMPNFSTIFFFFTLANM 389

Query: 520 SLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 579
           SLPGTSSFIGEF I VGAFQRNSLVATL ALGMILGAAYSLWLYNRVVSGNLKPDFL+KF
Sbjct: 390 SLPGTSSFIGEFLILVGAFQRNSLVATLRALGMILGAAYSLWLYNRVVSGNLKPDFLYKF 449

Query: 580 SDPNGREVSIFIPFLVG 597
           SD NGREV IF+PFLVG
Sbjct: 450 SDLNGREVFIFLPFLVG 466

[Close]

BLAST of Spg009304 vs. ExPASy Swiss-Prot
Match: P26848 (NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha OX=3197 GN=ND4 PE=3 SV=2)

HSP 1 Score: 216.1 bits (549), Expect = 1.1e-54
Identity = 112/137 (81.75%), Postives = 119/137 (86.86%), Query Frame = 0


Query: 460 GIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANM 519
           GI GSIL MLSHGLV SALFLCVG LYDRH+TR+V+YYGG VSTMP  STI   FTLANM
Sbjct: 330 GIEGSILLMLSHGLVSSALFLCVGALYDRHKTRIVKYYGGLVSTMPIFSTIFLFFTLANM 389

Query: 520 SLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 579
           SLPGTSSFIGEF I VGAFQRNSLVATLAALGMILGAAYSLWLYNRVV GN KP+F+ KF
Sbjct: 390 SLPGTSSFIGEFLILVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVFGNFKPNFILKF 449

Query: 580 SDPNGREVSIFIPFLVG 597
           SD N REV IF+PF+VG
Sbjct: 450 SDLNRREVLIFLPFIVG 466

[Close]

BLAST of Spg009304 vs. ExPASy Swiss-Prot
Match: Q37617 (NADH-ubiquinone oxidoreductase chain 4 OS=Prototheca wickerhamii OX=3111 GN=ND4 PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 9.0e-41
Identity = 84/140 (60.00%), Postives = 105/140 (75.00%), Query Frame = 0


Query: 457 STSGIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTL 516
           +T GI GSIL M+SHGLV  ALFLCVGVLYDRH+TRL+RYY G   TMP  + +   FT+
Sbjct: 358 NTQGIEGSILLMISHGLVSPALFLCVGVLYDRHKTRLLRYYSGCGQTMPIFALLFVFFTM 417

Query: 517 ANMSLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFL 576
           AN+SLPGTSSF GEF + +G++Q NS VA  AA GM+LGAAY+LWL NR++ G  KPDF+
Sbjct: 418 ANISLPGTSSFPGEFLVFIGSYQNNSFVAFCAATGMVLGAAYALWLCNRLIYGVSKPDFI 477

Query: 577 HKFSDPNGREVSIFIPFLVG 597
           + +SD N RE  +F P + G
Sbjct: 478 NTWSDVNRREFFMFAPLIAG 497

[Close]

BLAST of Spg009304 vs. ExPASy TrEMBL
Match: A0A5A7UB26 (NADH-quinone oxidoreductase protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold48939G00010 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.3e-90
Identity = 220/423 (52.01%), Postives = 220/423 (52.01%), Query Frame = 0


Query: 188 PLASSFLRQLPFIRFAGAAQHIAGNLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTAVK 247
           P   SF   LPFIRFAGAAQHIAGNLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTAVK
Sbjct: 17  PWGGSF--SLPFIRFAGAAQHIAGNLTLSTLGANPLSVLPPRRRTGARVEPFLSGVTAVK 76

Query: 248 RNKILRSRNRRAGGKALLWSRTPRVLSSLQRLASFPFLFRSDNENAALSFSCRGRTSACG 307
                      A GK                                             
Sbjct: 77  -----------AVGK--------------------------------------------- 136

Query: 308 VVQPMGGVALIPPIAQCSLLLRRALPVPGVDAGFYKRMRSSQHKKTRTARSPPEFVFRRH 367
                                                                       
Sbjct: 137 ------------------------------------------------------------ 196

Query: 368 RPAAGETQPGPLSGNGGPTSTCCRGGPQAPGPPLPSSSVKPTSFSPVLTHRGELLLGREE 427
                                                                  LGREE
Sbjct: 197 -------------------------------------------------------LGREE 256

Query: 428 GKGLGLPIPIGPHKGTGAVERFHIAEPKGSTS-----------------------GIGGS 487
           GKGLGLPIPIGPHKG GAVERFHIAEPKGSTS                       GIGGS
Sbjct: 257 GKGLGLPIPIGPHKGRGAVERFHIAEPKGSTSLRDRSISPPLVPAAFHSIYALFPGIGGS 266

Query: 488 ILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGT 547
           ILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGT
Sbjct: 317 ILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGT 266

Query: 548 SSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDPNG 588
           SSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGN KPDFLHKFSDPNG
Sbjct: 377 SSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNCKPDFLHKFSDPNG 266

[Close]

BLAST of Spg009304 vs. ExPASy TrEMBL
Match: A0A2P6P129 (NADH-ubiquinone oxidoreductase chain 4 OS=Rosa chinensis OX=74649 GN=RchiOBHm_MTg0498651 PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 6.6e-87
Identity = 170/188 (90.43%), Postives = 173/188 (92.02%), Query Frame = 0


Query: 422 LLGREEGKGLGLPIPIGPHKGTGAVERFHIAEPKGSTS----------GIGGSILPMLSH 481
           +L RE GKGLGLPIPIGPHKGTGAVERFHIAEPKGSTS          GIGGSILPMLSH
Sbjct: 45  VLWREGGKGLGLPIPIGPHKGTGAVERFHIAEPKGSTSIRDRSMSRRLGIGGSILPMLSH 104

Query: 482 GLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGTSSFIGEF 541
           GLVPSALFLCVGVLYDRH+TRLVRYYGGSVSTMPNLSTISFS TLANMS PGTSSFIGEF
Sbjct: 105 GLVPSALFLCVGVLYDRHKTRLVRYYGGSVSTMPNLSTISFSSTLANMSSPGTSSFIGEF 164

Query: 542 PISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDPNGREVSIFI 600
           PI VGAFQRNSLVATLAALGMILGAAYSLWLYNR+VSGNLKPDFLHKFSDPNGREVSIFI
Sbjct: 165 PILVGAFQRNSLVATLAALGMILGAAYSLWLYNRLVSGNLKPDFLHKFSDPNGREVSIFI 224

[Close]

BLAST of Spg009304 vs. ExPASy TrEMBL
Match: A0A072THH4 (NADH-quinone oxidoreductase protein OS=Medicago truncatula OX=3880 GN=MTR_0082s0060 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 3.9e-79
Identity = 160/186 (86.02%), Postives = 162/186 (87.10%), Query Frame = 0


Query: 430 GLGLPIPIGPHKGTGAVERFHIAEPKGSTSGIGG--------------SILPMLSHGLVP 489
           GLGLPIPIGPHKGTGAV+RFHIAEPKGSTS                  SI PMLSHGLVP
Sbjct: 8   GLGLPIPIGPHKGTGAVDRFHIAEPKGSTSVRDRSMSSRPRCIEKYLCSIPPMLSHGLVP 67

Query: 490 SALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANMSLPGTSSFIGEFPISV 549
           SALFLCVGVLYDRH+TRLVRYYGG VSTMPNLSTISFS TLANMS PGTSSFIGEFPISV
Sbjct: 68  SALFLCVGVLYDRHKTRLVRYYGGLVSTMPNLSTISFSSTLANMSSPGTSSFIGEFPISV 127

Query: 550 GAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKFSDPNGREVSIFIPFLV 602
           GAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLK DFLHKFSDPNGREVSIFIPFLV
Sbjct: 128 GAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKADFLHKFSDPNGREVSIFIPFLV 187

[Close]

BLAST of Spg009304 vs. ExPASy TrEMBL
Match: A0A4U5PYN3 (NADH-ubiquinone oxidoreductase chain 4 (Mitochondrion) OS=Populus alba OX=43335 GN=D5086_0000160910 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.6e-72
Identity = 160/217 (73.73%), Postives = 166/217 (76.50%), Query Frame = 0


Query: 396 APGPPLPSSSVKPTSFSPVLTHRGELLLGRE-EGKGLG--LPIPIGPHKGTGAVERFHIA 455
           A G P     +K       LT +      R  EGKG+G   P PIGPHKG+   ERFH A
Sbjct: 247 AKGAPPARLPIKEAESKRKLTRKPWGTTARSGEGKGVGPTYPDPIGPHKGSH--ERFHRA 306

Query: 456 E--------PKGSTSGIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTM 515
           E           +  GIGGSILPMLSHGLVPSALFLCVGVLYDRH+TRLVRYYGG VSTM
Sbjct: 307 EGLLGGESLALSNIQGIGGSILPMLSHGLVPSALFLCVGVLYDRHKTRLVRYYGGLVSTM 366

Query: 516 PNLSTISFSFTLANMSLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYN 575
           PNLSTI FS TLANMS PGTSSFIGEF ISVGAFQRNSLVATLAALGMILGAAYSLWLYN
Sbjct: 367 PNLSTIFFSSTLANMSSPGTSSFIGEFLISVGAFQRNSLVATLAALGMILGAAYSLWLYN 426

Query: 576 RVVSGNLKPDFLHKFSDPNGREVSIFIPFLVGGATAR 602
           RVVSGNLKPDFLHKFSD NGREVSIFIPFLVGGAT R
Sbjct: 427 RVVSGNLKPDFLHKFSDSNGREVSIFIPFLVGGATVR 461

[Close]

BLAST of Spg009304 vs. ExPASy TrEMBL
Match: A0A7D5Y3R4 (NADH-ubiquinone oxidoreductase chain 4 OS=Luffa acutangula OX=56866 GN=nad4 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 8.1e-69
Identity = 136/137 (99.27%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 460 GIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANM 519
           GIGGSILPMLSHGLVPSALFLCVGVLYDRH+TRLVRYYGGSVSTMPNLSTISFSFTLANM
Sbjct: 330 GIGGSILPMLSHGLVPSALFLCVGVLYDRHKTRLVRYYGGSVSTMPNLSTISFSFTLANM 389

Query: 520 SLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 579
           SLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF
Sbjct: 390 SLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 449

Query: 580 SDPNGREVSIFIPFLVG 597
           SDPNGREVSIFIPFLVG
Sbjct: 450 SDPNGREVSIFIPFLVG 466

[Close]

BLAST of Spg009304 vs. TAIR 10
Match: ATMG00580.1 (NADH dehydrogenase subunit 4 )

HSP 1 Score: 255.4 bits (651), Expect = 1.2e-67
Identity = 130/137 (94.89%), Postives = 131/137 (95.62%), Query Frame = 0


Query: 460 GIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANM 519
           GIGGSILPMLSHGLVPSALFLCVGVLYDRH+TRLVRYYGG VSTMPNLSTI FSFTLANM
Sbjct: 330 GIGGSILPMLSHGLVPSALFLCVGVLYDRHKTRLVRYYGGLVSTMPNLSTIFFSFTLANM 389

Query: 520 SLPGTSSFIGEFPISVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 579
           S PGTSSFIGEF I VGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF
Sbjct: 390 SSPGTSSFIGEFLILVGAFQRNSLVATLAALGMILGAAYSLWLYNRVVSGNLKPDFLHKF 449

Query: 580 SDPNGREVSIFIPFLVG 597
           SD NGREVSIFIPFLVG
Sbjct: 450 SDSNGREVSIFIPFLVG 466

[Close]

BLAST of Spg009304 vs. TAIR 10
Match: ATCG01050.1 (NADH-Ubiquinone/plastoquinone (complex I) protein )

HSP 1 Score: 65.1 bits (157), Expect = 2.2e-10
Identity = 46/147 (31.29%), Postives = 76/147 (51.70%), Query Frame = 0


Query: 460 GIGGSILPMLSHGLVPSALFLCVGVLYDRHQTRLVRYYGGSVSTMPNLSTISFSFTLANM 519
           G+ G+IL ++SHG + +ALF   G  YDR +   +   GG   ++P + T+    ++A++
Sbjct: 333 GLNGAILQIISHGFIGAALFFLAGTSYDRIRLVYLDEMGGMAISIPKIFTMFTILSMASL 392

Query: 520 SLPGTSSFIGEFPISVGAF--QRNSLVATL-----AALGMILGAAYSLWLYNRVVSG--- 579
           +LPG S FI EF +  G    Q+  L++ +      A+GMIL   Y L +  ++  G   
Sbjct: 393 ALPGMSGFIAEFIVFFGIITSQKYFLISKIFIIFVMAIGMILTPIYLLSMLRQMFYGYKL 452

Query: 580 -NLKPDFLHKFSDPNGREVSIFIPFLV 596
            N+K      F D   RE+ + I  L+
Sbjct: 453 INIKN---FSFFDSGPRELFLSISILL 476

The following BLAST results are available for this feature:

BLAST of Spg009304 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0052380.1	2.7e-90	52.01	NADH-quinone oxidoreductase protein [Cucumis melo var. makuwa]	[more]
PRQ15629.1	1.4e-86	90.43	putative NADH:ubiquinone reductase (H(+)-translocating) [Rosa chinensis]	[more]
KEH16847.1	8.0e-79	86.02	NADH-quinone oxidoreductase protein [Medicago truncatula]	[more]
KAG6585907.1	4.1e-75	43.91	NADH-ubiquinone oxidoreductase chain 4, partial [Cucurbita argyrosperma subsp. s...	[more]
TKS02690.1	3.3e-72	73.73	NADH-ubiquinone oxidoreductase chain 4 (mitochondrion) [Populus alba]	[more]

BLAST of Spg009304 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q04050	1.1e-67	95.62	NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris OX=3711 GN=ND4 PE=...	[more]
P93313	9.9e-64	92.70	NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana OX=3702 GN=ND4 PE...	[more]
P27572	1.6e-61	89.78	NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum OX=4565 GN=ND4 PE=2 ...	[more]
P26848	1.1e-54	81.75	NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha OX=3197 GN=ND4 P...	[more]
Q37617	9.0e-41	60.00	NADH-ubiquinone oxidoreductase chain 4 OS=Prototheca wickerhamii OX=3111 GN=ND4 ...	[more]

BLAST of Spg009304 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UB26	1.3e-90	52.01	NADH-quinone oxidoreductase protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A2P6P129	6.6e-87	90.43	NADH-ubiquinone oxidoreductase chain 4 OS=Rosa chinensis OX=74649 GN=RchiOBHm_MT...	[more]
A0A072THH4	3.9e-79	86.02	NADH-quinone oxidoreductase protein OS=Medicago truncatula OX=3880 GN=MTR_0082s0...	[more]
A0A4U5PYN3	1.6e-72	73.73	NADH-ubiquinone oxidoreductase chain 4 (Mitochondrion) OS=Populus alba OX=43335 ...	[more]
A0A7D5Y3R4	8.1e-69	99.27	NADH-ubiquinone oxidoreductase chain 4 OS=Luffa acutangula OX=56866 GN=nad4 PE=3...	[more]

BLAST of Spg009304 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00580.1	1.2e-67	94.89	NADH dehydrogenase subunit 4	[more]
ATCG01050.1	2.2e-10	31.29	NADH-Ubiquinone/plastoquinone (complex I) protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003918	NADH:ubiquinone oxidoreductase	PRINTS	PR01437	NUOXDRDTASE4	coord: 512..538 score: 38.38 coord: 460..479 score: 49.77
IPR003918	NADH:ubiquinone oxidoreductase	PANTHER	PTHR43507	NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4	coord: 464..599
IPR001750	NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit	PFAM	PF00361	Proton_antipo_M	coord: 459..552 e-value: 4.3E-13 score: 49.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..40
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 354..368
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 346..410
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 150..169
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR43507:SF13	NADH-QUINONE OXIDOREDUCTASE PROTEIN	coord: 464..599

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg009304.1	Spg009304.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042773	ATP synthesis coupled electron transport
biological_process	GO:0015990	electron transport coupled proton transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0031966	mitochondrial membrane
cellular_component	GO:0070469	respirasome
molecular_function	GO:0008137	NADH dehydrogenase (ubiquinone) activity
molecular_function	GO:0048039	ubiquinone binding