Spg009065 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg009065
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCysteine synthase
Locationscaffold2: 28529386 .. 28532972 (-)
RNA-Seq ExpressionSpg009065
SyntenySpg009065
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAACAGAAAATCAGCTAAATTTACCAAATCACTTTAACAGATAGGTTAAAAAGAAAATTGCAGAATAATCAAAAACAACAAGCAGATAAAGTCAAAACCGAGAGGCTAGGCTGGCCGGCTGTCAATTTTTTGGGCTTTGAAAGTCTCCTGCATTACTCCATGGCGCCCAAGGGTTTCGATTCATAAATAAGCTTTTTGAAGGCTTTGTGAGCTGAATCCTTGTGGGTTTTTCTTGTTGAAGGGATTTCTGAATCCAAGGTTTGTTTCTTTTCTTAGGGAAAGTTTAGGTCTGTCATCTAGATTATTAATTGCTGGACCATGTTCTTTAAGAAAAGTATCCATGTATTAATAATGGCTGATGCCTGAATGGTCCCTCGTTTCTTTCTTCTTTGGTTTTCAGTTCTTTCTGAAAATTGCAAGTACAAACTAAAGCATGGAGGAGAAGGCTGGAATCAAGAAAGATATCACTGAAGTAATTACCTCTTTCTATGAGTGGGTTGAATTTTTTAAACTGGTTTTCATGCTTGTTGTTGAGTTTTGCTCACTTTAACAACGTTCCTTCATTTCTGTCTCTTTTGTTCTTCTGGGTCTGGTTGGGATTCTTGCAGTTGATAGGTAATACTCCAATGGTGTATCTAAGTAAAGTGGCTGATGGTTGTTTTGCTCGTATCGCTGCTAAGTTAGAGACGATGGAGCCGTGTTCTAGTGTTAAAGATAGGTTTGAAGTCTATCTTTTAAAGATTATGATTACTTACCCTTTGTCTTGGCTAAGATTTGATTGCTGCTTGTAGAATGCAAATAGCTTAAACAGAGAAAATTTAGAACCAAAAACAAAATTGATATCAAATGGAGGGTTTTTCGAGATATGAACTGTACAAGTTTTCGATGTAGGATTGCGCACAGTATGATTCAAGATGCAGAGGATAATGGTCTGATTACACCAGGAAAGGTAGTATAAAATGCTTCATTTCTTTCTCTTACCATTTGAAATTAGGCCACCACTTGAATTGGCAATAGATTTTTGTGCTTGGTGTAATTACAATGCCTTGTAAAACAATTGTTGTGACACCCGAGGTTAGGAGGGGTACATGAATGAACCGAGCTCACATCCGAATGAGAGGGATCCTAAGGACATGAAAGCATGATCAAGATAAGCTTAAAAGAATTGATAGATGCTACCTATACCAACAAGGTGCACCTTTCTTTTTGGTGGCTCAATCATAGGAATTTTGAAGTTAAGTGTGTTTGGCTTGGAGAAGTTCTATGTTGGGTGACCTCCTAGGAATTTTCCTAGGAAGCATGTGAGTGAGGACAAAACATGTTAAAAGGACCCCGTGTTGATTTGTGAGGATAGTCTTCACTCTTGGAAGCAGTTTAAGACGCGTATGGTAACGTAACTTGAAGCTTATATATATTTGATCTGCTACCTGATCTGCCTTATGTTTTCTTTTTTTGGTGGAAAAAGACTGTCCTCGTCGAGGTTACGAGCGGTAACACGGGCATTGCGTTGGCGAGCATTGCAGCGGTGAAGGGATATAGACTTATTGTAGTTATGCCAGCGTCGTATAGTCTTGAAAGACGGATTCTGCTTCGAGCTTTTGGAGCCGAGCTGCACATCACAGACCCTGCAGCAGGGCTTGAAGGCCTTCTAAAGAAGGTTGAGGAGATAATGAAAGTCACACCCAATAGCTATTTTCTGAAGCAATTTGAAAATCCAGCAAACCCAAAGGTATGTTTTGTTTTGCTTTGTGATCTCAAAAGGATAGAACTCTGTGTTTTCAATTATGAATTGAGTCATTTTGCTTGCATCCATTATTGTTCTTTAGATTCATTATGAAACCACTGGGCCAGAGATATGGAAAGATTCGGGAGGAAAAGTTGATGCTTTGGTCGTGGGGATTGGAACCGGGGGAACCATAACTGGTGCAGGGAATTTTCTTAAGGAACAGAACCCAGACATTAAGGTTCTCTTGTTAGTTTGATGCACTAAACAAGACATTATTGGATCATTTGGTAACATTCTCATTTCTTATTATTCAAGAGATCAACTGTTATGTATGGTGTAGGTTTATGCAGTGGAACCAGCAGAGAGTGCAGTGCTTAGTGGAGGACAACCTGGTGAGTTTTGTTGGGAGTGCGACCAAAGTCCCACATTGGCTAGATAATGGGAGGATCATGGACTTATAAGGGAGGATAAGTATCTCTATTGGTATGGGGTCTTTTGGGGTGAACCAAAAGCAAAGCCATGAAGGTTTATGTCCAAAGTGGACAATATCATACCATTGTGGAGATCTGTGCCGTGTCCGTTGTCCCTTACAAGTTTCCATTCTAAAGCATATTTCATTATTTTTGGCCCTTCAATTTTCTTCAAATGGTTTACAAGTCACGTTTTACGTTTGATTGAACATAAATATCAAAACATTACTATGGAATTGGTGCTTCTCTTTGAAGTGCTTGTCTTAAAATCTGCATCTAAAATACTGATTTTTAACCAAAGTTTGTATACTAATATTTTCTTATGATGATTTCAGGCAAGCATCTCATTCAAGGAATTGGGGCTGGCTTCATCCCTCCTGTTCTTGATGTCAAAGTCTTTGATGAAGTCATTCAGGTGAAACTTGAACATATTTGTCCAAAGGAGTTCTTTCAATCTGTTCTCTATTTTTCAATTTTCTTTTAGATATGATTTAAACAATAATGATATGATGTGGCACTTTAAGATTACATGTAACATTGGCATATGCCTTTCCACTCAAATGTTAGTTTAAGAGATAGGACTTTCCCTTTTTCTTTTTTTGAGTCAAACAATACATCGGAGAGGGGATTGAACTCTTTGACCTTGTGGTCGACGTTATAATACTTTGATTAGTTGACTCATACTCAAGTTGAAGGCTTTTCTTTAAACTTAAAAGCAACGTTGAAGCTCTTATAGTTCCGAGGTCAGATAATGCATTTTGGTGTTTTTAGGTATCAAGTGAGGAAGCAATTGAAACTGCAAAGCTTCTTGCACTAAAAGAAGGCCTCCTAGTATGTTACTCAATCTATTAGACATTAATTTTTTGTGAAAATAGAGATTTAATTTTTTTTTCATTGAATTTGATGATTCTTTTTTTTTTTCTTTTGTAGGTGGGGATTTCATCAGGTGCAGCTGCAGCTGCTGCAGTAAAGGTTGCAAAGAGACCAGAAAGTGAAGGAAAACTCATTGTTGTAAGTCATATTTTACTTTCATTTGCAACAAATAAAAGATAAAATCTATGGTACAAAATTTGCAGCATATTTTAGATTGAAATATTTTGGATTTTGTGGTTCATTTGTAGGTCATCTTCCCAAGCTTCGGCGAGCGATATCTCTCGACAACACTTTTTGATTCCATTAGGCATGAAGCGGAAAACATGACTTATCTATGAGTTGATTTGGTTTGAATAATTTTCACATCTTCCTCTATCCAAATAAATATTGGGATTGGAATTCACTATATCAATAACCTTTGGTTGATGAAAATTGAAAAGTGATGATTTGAATAATATCCCACCATTACTAGCAAAATTTGGTAATAGAAATGGAGAAATACAAC

mRNA sequence

ATGGAGGAGAAGGCTGGAATCAAGAAAGATATCACTGAATTGATAGGTAATACTCCAATGGTGTATCTAAGTAAAGTGGCTGATGGTTGTTTTGCTCGTATCGCTGCTAAGTTAGAGACGATGGAGCCGTGTTCTAGTGTTAAAGATAGGATTGCGCACAGTATGATTCAAGATGCAGAGGATAATGGTCTGATTACACCAGGAAAGACTGTCCTCGTCGAGGTTACGAGCGGTAACACGGGCATTGCGTTGGCGAGCATTGCAGCGGTGAAGGGATATAGACTTATTGTAGTTATGCCAGCGTCGTATAGTCTTGAAAGACGGATTCTGCTTCGAGCTTTTGGAGCCGAGCTGCACATCACAGACCCTGCAGCAGGGCTTGAAGGCCTTCTAAAGAAGGTTGAGGAGATAATGAAAGTCACACCCAATAGCTATTTTCTGAAGCAATTTGAAAATCCAGCAAACCCAAAGATTCATTATGAAACCACTGGGCCAGAGATATGGAAAGATTCGGGAGGAAAAGTTGATGCTTTGGTCGTGGGGATTGGAACCGGGGGAACCATAACTGGTGCAGGGAATTTTCTTAAGGAACAGAACCCAGACATTAAGGTTTATGCAGTGGAACCAGCAGAGAGTGCAGTGCTTAGTGGAGGACAACCTGGCAAGCATCTCATTCAAGGAATTGGGGCTGGCTTCATCCCTCCTGTTCTTGATGTCAAAGTCTTTGATGAAGTCATTCAGGTATCAAGTGAGGAAGCAATTGAAACTGCAAAGCTTCTTGCACTAAAAGAAGGCCTCCTAGTGGGGATTTCATCAGGTGCAGCTGCAGCTGCTGCAGTAAAGGTTGCAAAGAGACCAGAAAGTGAAGGAAAACTCATTGTTGTCATCTTCCCAAGCTTCGGCGAGCGATATCTCTCGACAACACTTTTTGATTCCATTAGGCATGAAGCGGAAAACATGACTTATCTATGA

Coding sequence (CDS)

ATGGAGGAGAAGGCTGGAATCAAGAAAGATATCACTGAATTGATAGGTAATACTCCAATGGTGTATCTAAGTAAAGTGGCTGATGGTTGTTTTGCTCGTATCGCTGCTAAGTTAGAGACGATGGAGCCGTGTTCTAGTGTTAAAGATAGGATTGCGCACAGTATGATTCAAGATGCAGAGGATAATGGTCTGATTACACCAGGAAAGACTGTCCTCGTCGAGGTTACGAGCGGTAACACGGGCATTGCGTTGGCGAGCATTGCAGCGGTGAAGGGATATAGACTTATTGTAGTTATGCCAGCGTCGTATAGTCTTGAAAGACGGATTCTGCTTCGAGCTTTTGGAGCCGAGCTGCACATCACAGACCCTGCAGCAGGGCTTGAAGGCCTTCTAAAGAAGGTTGAGGAGATAATGAAAGTCACACCCAATAGCTATTTTCTGAAGCAATTTGAAAATCCAGCAAACCCAAAGATTCATTATGAAACCACTGGGCCAGAGATATGGAAAGATTCGGGAGGAAAAGTTGATGCTTTGGTCGTGGGGATTGGAACCGGGGGAACCATAACTGGTGCAGGGAATTTTCTTAAGGAACAGAACCCAGACATTAAGGTTTATGCAGTGGAACCAGCAGAGAGTGCAGTGCTTAGTGGAGGACAACCTGGCAAGCATCTCATTCAAGGAATTGGGGCTGGCTTCATCCCTCCTGTTCTTGATGTCAAAGTCTTTGATGAAGTCATTCAGGTATCAAGTGAGGAAGCAATTGAAACTGCAAAGCTTCTTGCACTAAAAGAAGGCCTCCTAGTGGGGATTTCATCAGGTGCAGCTGCAGCTGCTGCAGTAAAGGTTGCAAAGAGACCAGAAAGTGAAGGAAAACTCATTGTTGTCATCTTCCCAAGCTTCGGCGAGCGATATCTCTCGACAACACTTTTTGATTCCATTAGGCATGAAGCGGAAAACATGACTTATCTATGA

Protein sequence

MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAEDNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHITDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVVGIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVKVFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSFGERYLSTTLFDSIRHEAENMTYL
Homology
BLAST of Spg009065 vs. NCBI nr
Match: XP_038878751.1 (cysteine synthase-like isoform X1 [Benincasa hispida] >XP_038878752.1 cysteine synthase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 595.1 bits (1533), Expect = 3.6e-166
Identity = 305/323 (94.43%), Postives = 314/323 (97.21%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           MEEK GIKKDITELIGNTPMVYLSKVADGCFA IAAKLE MEPCSSVKDRI  SMIQDAE
Sbjct: 1   MEEKGGIKKDITELIGNTPMVYLSKVADGCFAHIAAKLEMMEPCSSVKDRIGQSMIQDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGKTVL+E+TSGNTGIALASIAA+KGY+LIVVMPASYSLERRILLRAFGAEL+I
Sbjct: 61  DKGLITPGKTVLIEITSGNTGIALASIAALKGYKLIVVMPASYSLERRILLRAFGAELYI 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENP+NPKIHYETTGPEIWKDSGGKVDALVV
Sbjct: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPSNPKIHYETTGPEIWKDSGGKVDALVV 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK
Sbjct: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           V+DEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKRPESEGKLIVVIFPS 
Sbjct: 241 VYDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAALKVAKRPESEGKLIVVIFPSS 300

Query: 301 GERYLSTTLFDSIRHEAENMTYL 324
           GERYLST LFDSIRHE ENMTY+
Sbjct: 301 GERYLSTMLFDSIRHEVENMTYV 323

BLAST of Spg009065 vs. NCBI nr
Match: XP_022153531.1 (cysteine synthase-like [Momordica charantia] >XP_022153532.1 cysteine synthase-like [Momordica charantia] >XP_022153533.1 cysteine synthase-like [Momordica charantia] >XP_022153534.1 cysteine synthase-like [Momordica charantia] >XP_022153535.1 cysteine synthase-like [Momordica charantia])

HSP 1 Score: 594.3 bits (1531), Expect = 6.1e-166
Identity = 307/322 (95.34%), Postives = 312/322 (96.89%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           MEEK GIK DITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE
Sbjct: 1   MEEKGGIKNDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           DNGLITP KTVL+EVTSGNT IALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI
Sbjct: 61  DNGLITPEKTVLIEVTSGNTXIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPA GLEGLLKK EEIM+VTP+ YFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV
Sbjct: 121 TDPALGLEGLLKKAEEIMQVTPDCYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITGAGNFLKEQN DIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK
Sbjct: 181 GIGTGGTITGAGNFLKEQNQDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           VFDEVIQVSSEE+IETAKLLALKEGLLVGISSGAAAAAA+KVAKRPES GKLIVVIFPSF
Sbjct: 241 VFDEVIQVSSEESIETAKLLALKEGLLVGISSGAAAAAAIKVAKRPESAGKLIVVIFPSF 300

Query: 301 GERYLSTTLFDSIRHEAENMTY 323
           GERYLSTTLFDSIR EAENMTY
Sbjct: 301 GERYLSTTLFDSIRKEAENMTY 322

BLAST of Spg009065 vs. NCBI nr
Match: XP_023001109.1 (cysteine synthase-like [Cucurbita maxima])

HSP 1 Score: 585.9 bits (1509), Expect = 2.2e-163
Identity = 296/323 (91.64%), Postives = 312/323 (96.59%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           MEEK+GIKKDITELIGNTPMVYL+K+ADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE
Sbjct: 1   MEEKSGIKKDITELIGNTPMVYLNKMADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGKTVLVE+TSGNTGIALA IAAVKGYRLI+VMPASYSLERRILL AFGAELHI
Sbjct: 61  DKGLITPGKTVLVEITSGNTGIALAGIAAVKGYRLIIVMPASYSLERRILLLAFGAELHI 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPA GLEGLLKKV EIM+ TPNSYFLKQF+NPANPKIHYETTGPEIWKDSGGKVD LVV
Sbjct: 121 TDPALGLEGLLKKVGEIMEATPNSYFLKQFDNPANPKIHYETTGPEIWKDSGGKVDVLVV 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITG GNFLKEQNPDIKVYAVEPAESAVLSG QPGKHLIQGIGAGFIP +LD+K
Sbjct: 181 GIGTGGTITGTGNFLKEQNPDIKVYAVEPAESAVLSGEQPGKHLIQGIGAGFIPAILDLK 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           VFDEV +V+SEEAIETA+LLALKEGLLVGISSGAAAAAA+KVAKRPE+EGKL+VVIFPSF
Sbjct: 241 VFDEVFKVTSEEAIETARLLALKEGLLVGISSGAAAAAAIKVAKRPENEGKLVVVIFPSF 300

Query: 301 GERYLSTTLFDSIRHEAENMTYL 324
           GERYLSTTLFDSIRH+AENMTYL
Sbjct: 301 GERYLSTTLFDSIRHQAENMTYL 323

BLAST of Spg009065 vs. NCBI nr
Match: XP_022933040.1 (cysteine synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 585.1 bits (1507), Expect = 3.7e-163
Identity = 296/323 (91.64%), Postives = 312/323 (96.59%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           MEEK+GIKKDITELIGNTPMVYL+K+ DGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE
Sbjct: 1   MEEKSGIKKDITELIGNTPMVYLNKMVDGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGKTVLVE+TSGNTGIALASIAAVKGYRLI+VMPASYSLERRILL AFGAELHI
Sbjct: 61  DKGLITPGKTVLVEITSGNTGIALASIAAVKGYRLIIVMPASYSLERRILLLAFGAELHI 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPA GLEGLLKKV E+M+ TPNSYFLKQF+NPANPKIHYETTGPEIWKDSGGKVD LVV
Sbjct: 121 TDPALGLEGLLKKVGEVMEATPNSYFLKQFDNPANPKIHYETTGPEIWKDSGGKVDVLVV 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITG GNFLKEQNPDIKVYAVEPAESA+LSG QPGKHLIQGIGAGFIP VLD+K
Sbjct: 181 GIGTGGTITGTGNFLKEQNPDIKVYAVEPAESAMLSGEQPGKHLIQGIGAGFIPAVLDLK 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           VFDEV +V+SEEAIETA+LLALKEGLLVGISSGAAAAAA+KVAKRPE+EGKLIVVIFPSF
Sbjct: 241 VFDEVFKVTSEEAIETARLLALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSF 300

Query: 301 GERYLSTTLFDSIRHEAENMTYL 324
           GERYLSTTLFDSIRH+AENMTYL
Sbjct: 301 GERYLSTTLFDSIRHQAENMTYL 323

BLAST of Spg009065 vs. NCBI nr
Match: XP_023531920.1 (cysteine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 297/323 (91.95%), Postives = 312/323 (96.59%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           MEEK+GIKKDITELIGNTPMVYL+K+ADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE
Sbjct: 1   MEEKSGIKKDITELIGNTPMVYLNKMADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGKTVLVE+TSGNTGIALASIAAVKGYRLI+VMPASYSLERRILL AFGAELHI
Sbjct: 61  DKGLITPGKTVLVEITSGNTGIALASIAAVKGYRLIIVMPASYSLERRILLLAFGAELHI 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPA GLEGLLKKV EIM+ TPNS FLKQF+NPANPKIHYETTGPEIWKDSGGKVD LVV
Sbjct: 121 TDPALGLEGLLKKVGEIMEATPNSCFLKQFDNPANPKIHYETTGPEIWKDSGGKVDVLVV 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITG GNFLKEQNPDIKVYAVEPAESAVLSG QPGKHLIQGIGAGFIP VLD+K
Sbjct: 181 GIGTGGTITGTGNFLKEQNPDIKVYAVEPAESAVLSGEQPGKHLIQGIGAGFIPAVLDLK 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           VFDEV +V+SEEAIETA+LLA+KEGLLVGISSGAAAAAA+KVAKRPE+EGKLIVVIFPSF
Sbjct: 241 VFDEVFKVTSEEAIETARLLAVKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSF 300

Query: 301 GERYLSTTLFDSIRHEAENMTYL 324
           GERYLSTTLFDSIRH+AENMTYL
Sbjct: 301 GERYLSTTLFDSIRHQAENMTYL 323

BLAST of Spg009065 vs. ExPASy Swiss-Prot
Match: Q43317 (Cysteine synthase OS=Citrullus lanatus OX=3654 PE=1 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 7.3e-138
Identity = 242/321 (75.39%), Postives = 281/321 (87.54%), Query Frame = 0

Query: 2   EEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAED 61
           + K+ I KD+TELIGNTP+VYL++V DGC AR+AAKLE MEPCSSVKDRI +SMI DAE+
Sbjct: 3   DAKSTIAKDVTELIGNTPLVYLNRVVDGCVARVAAKLEMMEPCSSVKDRIGYSMISDAEN 62

Query: 62  NGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHIT 121
            GLITPG++VL+E TSGNTGI LA IAA KGYRLI+ MPAS SLERR +LRAFGAEL +T
Sbjct: 63  KGLITPGESVLIEPTSGNTGIGLAFIAAAKGYRLIICMPASMSLERRTILRAFGAELVLT 122

Query: 122 DPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVVG 181
           DPA G++G ++K EEI   TPNSY L+QFENPANPKIHYETTGPEIW+ SGGK+DALV G
Sbjct: 123 DPARGMKGAVQKAEEIKAKTPNSYILQQFENPANPKIHYETTGPEIWRGSGGKIDALVSG 182

Query: 182 IGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVKV 241
           IGTGGT+TGAG +LKEQNP+IK+Y VEP ESA+LSGG+PG H IQGIGAGFIP VLDV +
Sbjct: 183 IGTGGTVTGAGKYLKEQNPNIKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPGVLDVNL 242

Query: 242 FDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSFG 301
            DEVIQVSSEE+IETAKLLALKEGLLVGISSGAAAAAA+++AKRPE+ GKLIV +FPSFG
Sbjct: 243 LDEVIQVSSEESIETAKLLALKEGLLVGISSGAAAAAAIRIAKRPENAGKLIVAVFPSFG 302

Query: 302 ERYLSTTLFDSIRHEAENMTY 323
           ERYLST LF+S++ E ENM +
Sbjct: 303 ERYLSTVLFESVKRETENMVF 323

BLAST of Spg009065 vs. ExPASy Swiss-Prot
Match: Q00834 (Cysteine synthase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 2.6e-135
Identity = 240/320 (75.00%), Postives = 277/320 (86.56%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           +EEKA I KD+TELIG TP+VYL+ VADGC AR+AAKLE MEPCSSVKDRI  SMI DAE
Sbjct: 2   VEEKAFIAKDVTELIGKTPLVYLNTVADGCVARVAAKLEGMEPCSSVKDRIGFSMITDAE 61

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
            +GLITPG++VL+E TSGNTGI LA IAA KGY+LI+ MPAS SLERR +LRAFGAEL +
Sbjct: 62  KSGLITPGESVLIEPTSGNTGIGLAFIAAAKGYKLIITMPASMSLERRTILRAFGAELIL 121

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPA G++G ++K EEI   TPNSY L+QFENPANPK+HYETTGPEIWK +GGK+D  V 
Sbjct: 122 TDPAKGMKGAVQKAEEIRDKTPNSYILQQFENPANPKVHYETTGPEIWKGTGGKIDIFVS 181

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITGAG +LKEQNPD+K+  +EP ESAVLSGG+PG H IQG+GAGFIP VLDV 
Sbjct: 182 GIGTGGTITGAGKYLKEQNPDVKLIGLEPVESAVLSGGKPGPHKIQGLGAGFIPGVLDVN 241

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           + DEV+Q+SSEE+IE AKLLALKEGLLVGISSGAAAAAA+KVAKRPE+ GKLIV +FPSF
Sbjct: 242 IIDEVVQISSEESIEMAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGKLIVAVFPSF 301

Query: 301 GERYLSTTLFDSIRHEAENM 321
           GERYLS+ LFDS+R EAE+M
Sbjct: 302 GERYLSSVLFDSVRKEAESM 321

BLAST of Spg009065 vs. ExPASy Swiss-Prot
Match: O81154 (Cysteine synthase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 2.2e-134
Identity = 236/319 (73.98%), Postives = 277/319 (86.83%), Query Frame = 0

Query: 3   EKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAEDN 62
           EK GI KD+TELIGNTP+VYL+ V DGC AR+AAKLE+MEPCSSVKDRI +SMI DAE+ 
Sbjct: 4   EKIGIAKDVTELIGNTPLVYLNNVVDGCVARVAAKLESMEPCSSVKDRIGYSMITDAEEK 63

Query: 63  GLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHITD 122
           GLI PG++VL+E TSGNTG+ LA +AA KGY+LI+ MP+S SLERRI+LR F +EL +TD
Sbjct: 64  GLIKPGESVLIEPTSGNTGVGLAFMAAAKGYKLIITMPSSMSLERRIILRGFRSELVLTD 123

Query: 123 PAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVVGI 182
           PA G++G + K EEI   TPNSY L+QFENPANPKIHYETTGPEIWK S GKVDAL  GI
Sbjct: 124 PAKGMKGAISKAEEIKAKTPNSYILQQFENPANPKIHYETTGPEIWKGSNGKVDALASGI 183

Query: 183 GTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVKVF 242
           GTGGTITG+G +L+EQNP++K+Y VEP ESA+LSGG+PG H IQGIGAGFIP VL+V + 
Sbjct: 184 GTGGTITGSGKYLREQNPNVKLYGVEPVESAILSGGKPGPHKIQGIGAGFIPGVLEVNLI 243

Query: 243 DEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSFGE 302
           D+V+QVSSEE+IE AKLLALKEGLLVGISSGAAAAAA+KVAKRPE+ GKLIVVIFPSFGE
Sbjct: 244 DDVVQVSSEESIEMAKLLALKEGLLVGISSGAAAAAAIKVAKRPENAGKLIVVIFPSFGE 303

Query: 303 RYLSTTLFDSIRHEAENMT 322
           RYLS+ LF+++R EAENMT
Sbjct: 304 RYLSSVLFETVRREAENMT 322

BLAST of Spg009065 vs. ExPASy Swiss-Prot
Match: Q9XEA6 (Cysteine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=RCS1 PE=2 SV=2)

HSP 1 Score: 471.5 bits (1212), Expect = 7.8e-132
Identity = 232/314 (73.89%), Postives = 273/314 (86.94%), Query Frame = 0

Query: 7   IKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAEDNGLIT 66
           I KD+TELIGNTP+VYL++V DGC  R+AAKLE+MEPCSSVKDRI +SMI DAE+ GLIT
Sbjct: 5   IAKDVTELIGNTPLVYLNRVTDGCVGRVAAKLESMEPCSSVKDRIGYSMITDAEEKGLIT 64

Query: 67  PGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHITDPAAG 126
           PGK+VL+E TSGNTGI LA +AA KGYRL++ MPAS S+ERRI+L+AFGAEL +TDP  G
Sbjct: 65  PGKSVLIEPTSGNTGIGLAFMAAAKGYRLVLTMPASMSMERRIILKAFGAELILTDPLLG 124

Query: 127 LEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVVGIGTGG 186
           ++G ++K EE+   T NS+ L+QFENPANPKIHYETTGPEIWK +GGKVD LV GIGTGG
Sbjct: 125 MKGAVQKAEELAAKTNNSFILQQFENPANPKIHYETTGPEIWKGTGGKVDGLVSGIGTGG 184

Query: 187 TITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVKVFDEVI 246
           TITGAG +L+EQNPDIK+Y VEP ESAVLSGG+PG H IQGIGAGF+P VLDV + +E +
Sbjct: 185 TITGAGRYLREQNPDIKIYGVEPVESAVLSGGKPGPHKIQGIGAGFVPGVLDVDLINETV 244

Query: 247 QVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSFGERYLS 306
           QVSS+EAIE AK LALKEGLLVGISSGAAAAAAV++A+RPE+EGKL VV+FPSFGERYLS
Sbjct: 245 QVSSDEAIEMAKALALKEGLLVGISSGAAAAAAVRLAQRPENEGKLFVVVFPSFGERYLS 304

Query: 307 TTLFDSIRHEAENM 321
           + LF SI+ EAENM
Sbjct: 305 SVLFQSIKKEAENM 318

BLAST of Spg009065 vs. ExPASy Swiss-Prot
Match: Q9SXS7 (Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 OS=Arabidopsis thaliana OX=3702 GN=CYSD2 PE=1 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.1e-130
Identity = 234/320 (73.12%), Postives = 271/320 (84.69%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           ME++  IK DITELIGNTPMVYL+ V DGC ARIAAKLE MEPCSSVKDRIA+SMI+DAE
Sbjct: 1   MEDRCLIKNDITELIGNTPMVYLNNVVDGCVARIAAKLEMMEPCSSVKDRIAYSMIKDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGK+ L+E T+GNTGI LA + A +GY++I+VMP++ SLERRI+LRA GAELH+
Sbjct: 61  DKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVILVMPSTMSLERRIILRALGAELHL 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           +D   GL+G+L+K E I+  TP  Y  +QFENPANP+IHY TTGPEIW+DS GKVD LV 
Sbjct: 121 SDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANPEIHYRTTGPEIWRDSAGKVDILVA 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           G+GTGGT TG G FLKEQN DIKV  VEP ES VLSGGQPG HLIQGIG+G +P  LD+ 
Sbjct: 181 GVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLSGGQPGPHLIQGIGSGIVPFNLDLT 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           + DE+IQV+ EEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKRPE+ GKLIVV+FPS 
Sbjct: 241 IVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAAAAALKVAKRPENAGKLIVVVFPSG 300

Query: 301 GERYLSTTLFDSIRHEAENM 321
           GERYLST LFDSIR+EAEN+
Sbjct: 301 GERYLSTKLFDSIRYEAENL 320

BLAST of Spg009065 vs. ExPASy TrEMBL
Match: A0A6J1DJ64 (Cysteine synthase OS=Momordica charantia OX=3673 GN=LOC111021015 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.9e-166
Identity = 307/322 (95.34%), Postives = 312/322 (96.89%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           MEEK GIK DITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE
Sbjct: 1   MEEKGGIKNDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           DNGLITP KTVL+EVTSGNT IALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI
Sbjct: 61  DNGLITPEKTVLIEVTSGNTXIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPA GLEGLLKK EEIM+VTP+ YFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV
Sbjct: 121 TDPALGLEGLLKKAEEIMQVTPDCYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITGAGNFLKEQN DIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK
Sbjct: 181 GIGTGGTITGAGNFLKEQNQDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           VFDEVIQVSSEE+IETAKLLALKEGLLVGISSGAAAAAA+KVAKRPES GKLIVVIFPSF
Sbjct: 241 VFDEVIQVSSEESIETAKLLALKEGLLVGISSGAAAAAAIKVAKRPESAGKLIVVIFPSF 300

Query: 301 GERYLSTTLFDSIRHEAENMTY 323
           GERYLSTTLFDSIR EAENMTY
Sbjct: 301 GERYLSTTLFDSIRKEAENMTY 322

BLAST of Spg009065 vs. ExPASy TrEMBL
Match: A0A6J1KLT7 (Cysteine synthase OS=Cucurbita maxima OX=3661 GN=LOC111495347 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.0e-163
Identity = 296/323 (91.64%), Postives = 312/323 (96.59%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           MEEK+GIKKDITELIGNTPMVYL+K+ADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE
Sbjct: 1   MEEKSGIKKDITELIGNTPMVYLNKMADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGKTVLVE+TSGNTGIALA IAAVKGYRLI+VMPASYSLERRILL AFGAELHI
Sbjct: 61  DKGLITPGKTVLVEITSGNTGIALAGIAAVKGYRLIIVMPASYSLERRILLLAFGAELHI 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPA GLEGLLKKV EIM+ TPNSYFLKQF+NPANPKIHYETTGPEIWKDSGGKVD LVV
Sbjct: 121 TDPALGLEGLLKKVGEIMEATPNSYFLKQFDNPANPKIHYETTGPEIWKDSGGKVDVLVV 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITG GNFLKEQNPDIKVYAVEPAESAVLSG QPGKHLIQGIGAGFIP +LD+K
Sbjct: 181 GIGTGGTITGTGNFLKEQNPDIKVYAVEPAESAVLSGEQPGKHLIQGIGAGFIPAILDLK 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           VFDEV +V+SEEAIETA+LLALKEGLLVGISSGAAAAAA+KVAKRPE+EGKL+VVIFPSF
Sbjct: 241 VFDEVFKVTSEEAIETARLLALKEGLLVGISSGAAAAAAIKVAKRPENEGKLVVVIFPSF 300

Query: 301 GERYLSTTLFDSIRHEAENMTYL 324
           GERYLSTTLFDSIRH+AENMTYL
Sbjct: 301 GERYLSTTLFDSIRHQAENMTYL 323

BLAST of Spg009065 vs. ExPASy TrEMBL
Match: A0A6J1F3U1 (Cysteine synthase OS=Cucurbita moschata OX=3662 GN=LOC111439729 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.8e-163
Identity = 296/323 (91.64%), Postives = 312/323 (96.59%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           MEEK+GIKKDITELIGNTPMVYL+K+ DGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE
Sbjct: 1   MEEKSGIKKDITELIGNTPMVYLNKMVDGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGKTVLVE+TSGNTGIALASIAAVKGYRLI+VMPASYSLERRILL AFGAELHI
Sbjct: 61  DKGLITPGKTVLVEITSGNTGIALASIAAVKGYRLIIVMPASYSLERRILLLAFGAELHI 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           TDPA GLEGLLKKV E+M+ TPNSYFLKQF+NPANPKIHYETTGPEIWKDSGGKVD LVV
Sbjct: 121 TDPALGLEGLLKKVGEVMEATPNSYFLKQFDNPANPKIHYETTGPEIWKDSGGKVDVLVV 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           GIGTGGTITG GNFLKEQNPDIKVYAVEPAESA+LSG QPGKHLIQGIGAGFIP VLD+K
Sbjct: 181 GIGTGGTITGTGNFLKEQNPDIKVYAVEPAESAMLSGEQPGKHLIQGIGAGFIPAVLDLK 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           VFDEV +V+SEEAIETA+LLALKEGLLVGISSGAAAAAA+KVAKRPE+EGKLIVVIFPSF
Sbjct: 241 VFDEVFKVTSEEAIETARLLALKEGLLVGISSGAAAAAAIKVAKRPENEGKLIVVIFPSF 300

Query: 301 GERYLSTTLFDSIRHEAENMTYL 324
           GERYLSTTLFDSIRH+AENMTYL
Sbjct: 301 GERYLSTTLFDSIRHQAENMTYL 323

BLAST of Spg009065 vs. ExPASy TrEMBL
Match: A0A0A0LWM5 (Cysteine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G574800 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 9.8e-162
Identity = 297/324 (91.67%), Postives = 313/324 (96.60%), Query Frame = 0

Query: 1   MEE-KAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDA 60
           MEE K GIKKD+TELIGNTPMVYLSK+ADGCFARIAAKLETMEPCSSVKDRI  SMI+DA
Sbjct: 53  MEEIKGGIKKDVTELIGNTPMVYLSKMADGCFARIAAKLETMEPCSSVKDRIGLSMIEDA 112

Query: 61  EDNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELH 120
           ED GLITPGKTVL+EVTSGNTGIALASIAAVKGY+LIVVMPASYSLERR+LL AFGAELH
Sbjct: 113 EDKGLITPGKTVLIEVTSGNTGIALASIAAVKGYKLIVVMPASYSLERRMLLLAFGAELH 172

Query: 121 ITDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALV 180
           ITDPAAGLEGLLKKVEEIM VTPNSYFLKQFENP+NPKIHYETTGPEIWKDSGGKVDALV
Sbjct: 173 ITDPAAGLEGLLKKVEEIMVVTPNSYFLKQFENPSNPKIHYETTGPEIWKDSGGKVDALV 232

Query: 181 VGIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDV 240
           VGIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSG +PGKHLIQGIG+GFIPPVLD+
Sbjct: 233 VGIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGEKPGKHLIQGIGSGFIPPVLDL 292

Query: 241 KVFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPS 300
           KV+DE+IQ+SSEEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKR ESEGKLIVV+FPS
Sbjct: 293 KVYDEIIQISSEEAIETAKLLALKEGLLVGISSGAAAAAAIKVAKRQESEGKLIVVVFPS 352

Query: 301 FGERYLSTTLFDSIRHEAENMTYL 324
            GERYLST LFDSIRHEAENMTY+
Sbjct: 353 SGERYLSTKLFDSIRHEAENMTYV 376

BLAST of Spg009065 vs. ExPASy TrEMBL
Match: A0A5D3CEF3 (Cysteine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003060 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.2e-161
Identity = 297/324 (91.67%), Postives = 312/324 (96.30%), Query Frame = 0

Query: 1   MEE-KAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDA 60
           MEE K GIKKD+TEL+GNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRI   MI+DA
Sbjct: 1   MEEIKGGIKKDVTELVGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIGLRMIEDA 60

Query: 61  EDNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELH 120
           ED GLITPGKTVL+EVTSGNTGIALASIAAVKGY+LIVVMPASYSLERR+LL AFGAELH
Sbjct: 61  EDKGLITPGKTVLIEVTSGNTGIALASIAAVKGYKLIVVMPASYSLERRMLLLAFGAELH 120

Query: 121 ITDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALV 180
           ITDPAAGLEGLLKKV+EIM+VTPNSYFLKQFENP+NPKIHYETTGPEIWKDSGGKVDALV
Sbjct: 121 ITDPAAGLEGLLKKVDEIMEVTPNSYFLKQFENPSNPKIHYETTGPEIWKDSGGKVDALV 180

Query: 181 VGIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDV 240
           VGIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSG QPGKHLIQGIGAGFIP VLD+
Sbjct: 181 VGIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGEQPGKHLIQGIGAGFIPAVLDL 240

Query: 241 KVFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPS 300
           KV+DE+IQVSSEEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKR ESEGKLIVV+FPS
Sbjct: 241 KVYDEIIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAIKVAKRQESEGKLIVVVFPS 300

Query: 301 FGERYLSTTLFDSIRHEAENMTYL 324
            GERYLST LFDSIRHEAENMTY+
Sbjct: 301 SGERYLSTKLFDSIRHEAENMTYV 324

BLAST of Spg009065 vs. TAIR 10
Match: AT5G28020.1 (cysteine synthase D2 )

HSP 1 Score: 467.6 bits (1202), Expect = 8.0e-132
Identity = 234/320 (73.12%), Postives = 271/320 (84.69%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           ME++  IK DITELIGNTPMVYL+ V DGC ARIAAKLE MEPCSSVKDRIA+SMI+DAE
Sbjct: 1   MEDRCLIKNDITELIGNTPMVYLNNVVDGCVARIAAKLEMMEPCSSVKDRIAYSMIKDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGK+ L+E T+GNTGI LA + A +GY++I+VMP++ SLERRI+LRA GAELH+
Sbjct: 61  DKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVILVMPSTMSLERRIILRALGAELHL 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           +D   GL+G+L+K E I+  TP  Y  +QFENPANP+IHY TTGPEIW+DS GKVD LV 
Sbjct: 121 SDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANPEIHYRTTGPEIWRDSAGKVDILVA 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           G+GTGGT TG G FLKEQN DIKV  VEP ES VLSGGQPG HLIQGIG+G +P  LD+ 
Sbjct: 181 GVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLSGGQPGPHLIQGIGSGIVPFNLDLT 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           + DE+IQV+ EEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKRPE+ GKLIVV+FPS 
Sbjct: 241 IVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAAAAALKVAKRPENAGKLIVVVFPSG 300

Query: 301 GERYLSTTLFDSIRHEAENM 321
           GERYLST LFDSIR+EAEN+
Sbjct: 301 GERYLSTKLFDSIRYEAENL 320

BLAST of Spg009065 vs. TAIR 10
Match: AT5G28020.2 (cysteine synthase D2 )

HSP 1 Score: 467.6 bits (1202), Expect = 8.0e-132
Identity = 234/320 (73.12%), Postives = 271/320 (84.69%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           ME++  IK DITELIGNTPMVYL+ V DGC ARIAAKLE MEPCSSVKDRIA+SMI+DAE
Sbjct: 1   MEDRCLIKNDITELIGNTPMVYLNNVVDGCVARIAAKLEMMEPCSSVKDRIAYSMIKDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGK+ L+E T+GNTGI LA + A +GY++I+VMP++ SLERRI+LRA GAELH+
Sbjct: 61  DKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVILVMPSTMSLERRIILRALGAELHL 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           +D   GL+G+L+K E I+  TP  Y  +QFENPANP+IHY TTGPEIW+DS GKVD LV 
Sbjct: 121 SDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANPEIHYRTTGPEIWRDSAGKVDILVA 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           G+GTGGT TG G FLKEQN DIKV  VEP ES VLSGGQPG HLIQGIG+G +P  LD+ 
Sbjct: 181 GVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLSGGQPGPHLIQGIGSGIVPFNLDLT 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           + DE+IQV+ EEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKRPE+ GKLIVV+FPS 
Sbjct: 241 IVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAAAAALKVAKRPENAGKLIVVVFPSG 300

Query: 301 GERYLSTTLFDSIRHEAENM 321
           GERYLST LFDSIR+EAEN+
Sbjct: 301 GERYLSTKLFDSIRYEAENL 320

BLAST of Spg009065 vs. TAIR 10
Match: AT5G28020.3 (cysteine synthase D2 )

HSP 1 Score: 467.6 bits (1202), Expect = 8.0e-132
Identity = 234/320 (73.12%), Postives = 271/320 (84.69%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           ME++  IK DITELIGNTPMVYL+ V DGC ARIAAKLE MEPCSSVKDRIA+SMI+DAE
Sbjct: 1   MEDRCLIKNDITELIGNTPMVYLNNVVDGCVARIAAKLEMMEPCSSVKDRIAYSMIKDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGK+ L+E T+GNTGI LA + A +GY++I+VMP++ SLERRI+LRA GAELH+
Sbjct: 61  DKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVILVMPSTMSLERRIILRALGAELHL 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           +D   GL+G+L+K E I+  TP  Y  +QFENPANP+IHY TTGPEIW+DS GKVD LV 
Sbjct: 121 SDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANPEIHYRTTGPEIWRDSAGKVDILVA 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           G+GTGGT TG G FLKEQN DIKV  VEP ES VLSGGQPG HLIQGIG+G +P  LD+ 
Sbjct: 181 GVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLSGGQPGPHLIQGIGSGIVPFNLDLT 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           + DE+IQV+ EEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKRPE+ GKLIVV+FPS 
Sbjct: 241 IVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAAAAALKVAKRPENAGKLIVVVFPSG 300

Query: 301 GERYLSTTLFDSIRHEAENM 321
           GERYLST LFDSIR+EAEN+
Sbjct: 301 GERYLSTKLFDSIRYEAENL 320

BLAST of Spg009065 vs. TAIR 10
Match: AT5G28020.4 (cysteine synthase D2 )

HSP 1 Score: 467.6 bits (1202), Expect = 8.0e-132
Identity = 234/320 (73.12%), Postives = 271/320 (84.69%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           ME++  IK DITELIGNTPMVYL+ V DGC ARIAAKLE MEPCSSVKDRIA+SMI+DAE
Sbjct: 1   MEDRCLIKNDITELIGNTPMVYLNNVVDGCVARIAAKLEMMEPCSSVKDRIAYSMIKDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGK+ L+E T+GNTGI LA + A +GY++I+VMP++ SLERRI+LRA GAELH+
Sbjct: 61  DKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVILVMPSTMSLERRIILRALGAELHL 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           +D   GL+G+L+K E I+  TP  Y  +QFENPANP+IHY TTGPEIW+DS GKVD LV 
Sbjct: 121 SDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANPEIHYRTTGPEIWRDSAGKVDILVA 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           G+GTGGT TG G FLKEQN DIKV  VEP ES VLSGGQPG HLIQGIG+G +P  LD+ 
Sbjct: 181 GVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLSGGQPGPHLIQGIGSGIVPFNLDLT 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           + DE+IQV+ EEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKRPE+ GKLIVV+FPS 
Sbjct: 241 IVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAAAAALKVAKRPENAGKLIVVVFPSG 300

Query: 301 GERYLSTTLFDSIRHEAENM 321
           GERYLST LFDSIR+EAEN+
Sbjct: 301 GERYLSTKLFDSIRYEAENL 320

BLAST of Spg009065 vs. TAIR 10
Match: AT5G28020.6 (cysteine synthase D2 )

HSP 1 Score: 467.6 bits (1202), Expect = 8.0e-132
Identity = 234/320 (73.12%), Postives = 271/320 (84.69%), Query Frame = 0

Query: 1   MEEKAGIKKDITELIGNTPMVYLSKVADGCFARIAAKLETMEPCSSVKDRIAHSMIQDAE 60
           ME++  IK DITELIGNTPMVYL+ V DGC ARIAAKLE MEPCSSVKDRIA+SMI+DAE
Sbjct: 1   MEDRCLIKNDITELIGNTPMVYLNNVVDGCVARIAAKLEMMEPCSSVKDRIAYSMIKDAE 60

Query: 61  DNGLITPGKTVLVEVTSGNTGIALASIAAVKGYRLIVVMPASYSLERRILLRAFGAELHI 120
           D GLITPGK+ L+E T+GNTGI LA + A +GY++I+VMP++ SLERRI+LRA GAELH+
Sbjct: 61  DKGLITPGKSTLIEPTAGNTGIGLACMGAARGYKVILVMPSTMSLERRIILRALGAELHL 120

Query: 121 TDPAAGLEGLLKKVEEIMKVTPNSYFLKQFENPANPKIHYETTGPEIWKDSGGKVDALVV 180
           +D   GL+G+L+K E I+  TP  Y  +QFENPANP+IHY TTGPEIW+DS GKVD LV 
Sbjct: 121 SDQRIGLKGMLEKTEAILSKTPGGYIPQQFENPANPEIHYRTTGPEIWRDSAGKVDILVA 180

Query: 181 GIGTGGTITGAGNFLKEQNPDIKVYAVEPAESAVLSGGQPGKHLIQGIGAGFIPPVLDVK 240
           G+GTGGT TG G FLKEQN DIKV  VEP ES VLSGGQPG HLIQGIG+G +P  LD+ 
Sbjct: 181 GVGTGGTATGVGKFLKEQNKDIKVCVVEPVESPVLSGGQPGPHLIQGIGSGIVPFNLDLT 240

Query: 241 VFDEVIQVSSEEAIETAKLLALKEGLLVGISSGAAAAAAVKVAKRPESEGKLIVVIFPSF 300
           + DE+IQV+ EEAIETAKLLALKEGLLVGISSGAAAAAA+KVAKRPE+ GKLIVV+FPS 
Sbjct: 241 IVDEIIQVAGEEAIETAKLLALKEGLLVGISSGAAAAAALKVAKRPENAGKLIVVVFPSG 300

Query: 301 GERYLSTTLFDSIRHEAENM 321
           GERYLST LFDSIR+EAEN+
Sbjct: 301 GERYLSTKLFDSIRYEAENL 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878751.13.6e-16694.43cysteine synthase-like isoform X1 [Benincasa hispida] >XP_038878752.1 cysteine s... [more]
XP_022153531.16.1e-16695.34cysteine synthase-like [Momordica charantia] >XP_022153532.1 cysteine synthase-l... [more]
XP_023001109.12.2e-16391.64cysteine synthase-like [Cucurbita maxima][more]
XP_022933040.13.7e-16391.64cysteine synthase-like isoform X1 [Cucurbita moschata][more]
XP_023531920.11.1e-16291.95cysteine synthase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q433177.3e-13875.39Cysteine synthase OS=Citrullus lanatus OX=3654 PE=1 SV=1[more]
Q008342.6e-13575.00Cysteine synthase OS=Spinacia oleracea OX=3562 PE=1 SV=1[more]
O811542.2e-13473.98Cysteine synthase OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q9XEA67.8e-13273.89Cysteine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=RCS1 PE=2 SV=2[more]
Q9SXS71.1e-13073.13Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 OS=Arabidopsis thali... [more]
Match NameE-valueIdentityDescription
A0A6J1DJ642.9e-16695.34Cysteine synthase OS=Momordica charantia OX=3673 GN=LOC111021015 PE=3 SV=1[more]
A0A6J1KLT71.0e-16391.64Cysteine synthase OS=Cucurbita maxima OX=3661 GN=LOC111495347 PE=3 SV=1[more]
A0A6J1F3U11.8e-16391.64Cysteine synthase OS=Cucurbita moschata OX=3662 GN=LOC111439729 PE=3 SV=1[more]
A0A0A0LWM59.8e-16291.67Cysteine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G574800 PE=3 SV=1[more]
A0A5D3CEF32.2e-16191.67Cysteine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003... [more]
Match NameE-valueIdentityDescription
AT5G28020.18.0e-13273.13cysteine synthase D2 [more]
AT5G28020.28.0e-13273.13cysteine synthase D2 [more]
AT5G28020.38.0e-13273.13cysteine synthase D2 [more]
AT5G28020.48.0e-13273.13cysteine synthase D2 [more]
AT5G28020.68.0e-13273.13cysteine synthase D2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 13..306
e-value: 1.3E-132
score: 443.4
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 49..151
e-value: 1.3E-132
score: 443.4
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 8..313
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 12..298
e-value: 2.8E-67
score: 227.2
IPR005859Cysteine synthase CysKTIGRFAMTIGR01139TIGR01139coord: 11..310
e-value: 7.9E-133
score: 440.2
IPR005856Cysteine synthaseTIGRFAMTIGR01136TIGR01136coord: 11..310
e-value: 2.1E-127
score: 422.6
NoneNo IPR availablePANTHERPTHR10314:SF205BIFUNCTIONAL L-3-CYANOALANINE SYNTHASE/CYSTEINE SYNTHASE D2coord: 1..322
NoneNo IPR availablePANTHERPTHR10314CYSTATHIONINE BETA-SYNTHASEcoord: 1..322
NoneNo IPR availableCDDcd01561CBS_likecoord: 16..306
e-value: 2.277E-137
score: 389.18
IPR001216Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment sitePROSITEPS00901CYS_SYNTHASEcoord: 37..55

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg009065.1Spg009065.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006535 cysteine biosynthetic process from serine
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004124 cysteine synthase activity