Spg008925 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008925
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionprotein NRT1/ PTR FAMILY 3.1-like
Locationscaffold5: 24627628 .. 24633103 (-)
RNA-Seq ExpressionSpg008925
SyntenySpg008925
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGGGAACGAAGTTTGTGATAGATTTGCAAGCACGGGTTTCCATGCGAACATAATAACATATTTGACACAAGATCTGAACATGGCTCTTGTTCCAGCCTCCAACATTCTCACCAACTTCGCTGCTACTTCTAGCTTCACTTCTCTCATCGGCGCTCTCATCGCCGACTCCTTCGCCGGCCGCTTCTCGACCATCATCGTTGCCTCCATCATCTATGAGCTCGTATATATACCCCTCTCTCTCGACACTGTTAAATTTCATTTTAGTTTCTAAACTTTCATTTTCCAACTTTCATTTCAAAAAGAAATTGTCAAAACTTGATTTTGGGCATCTTTGCAGCCCAGACACTTAGGCCAACCGTATAGCGCTACCACGTGTCAACGAAGTGTATGCTTTTATTTATTTATTTATTTTTAACGTTTTTTTTTCGGACTAAATTATAAAAAATGTCCCTGAACTATGGAGTTTGTTTAAAAAATGTCCCTCAACTTTGAAAAGTTTCAAAAATACCCCTGAACTTTGAAAAATGTTTCAAAAATACCACTGTCGTTAATTTTTGGATGAAAACCGTTAAAGTTTCAGTTTAAAAAATACCCTTGAACTTAAAAAAAAAATAATTTAAAATACCCTTATTGAAAAATGCTTCTTCTTTTTTCTCCTCTCACCGTAAATTCTAACGGTTTCCATCCAAAAATTAATGGCAAAGGTATTTTTAAAACATTTTTCAAAGTTCAGGGGTATTTTTGAAACTTTTAAAGTTTAGGAGCATTTTTTAAACACCATAGTTTAGAGGTATTTTTTTATAATTTAGCTTTTTTTTGTCTTTTTTATCTTTTCCTTTTTTGACAGCTCCTCATCTAGGGAAGAATAATACTTAACTGCATATTAATAGAGATCTTTTTACACATTTTATTAAAATGAATTTTTTTTGTTATCTCTCTTAATTGTCAACATTTTAATGGTGGTGTATAGCGAGATCCAAGTGTGCAATCAAGTATTCTCATTTAGGGAGTATATATTTCTATATGTCTATATTTCAAAATCGTACCATTTTTAACTAATCCATTCAAAATTGAAGGTTCCAAAATAATAATGTGAGAAAGATCTTAATGACTCTCACTTTTCAACTGGTTAAGTTAAAAATAAATATATTAATTATAGTCCAACTAGCAGTCAAGTTTTGCTCTTAATTGGATCTTCTAACTCTGAAGCTTATTCATTTTTTATCCTTTAGAAAAACTTTTTTTTTTAAGTAACCATTTCAGTTGTAGCTTCAAACATATAGTATTAATATGTTGATATAGACATGTGTATCAAAATCATATCATAAAATTGACTTAGCAAAATATCAAGATAAGTGAATACTAGTTAGCAAAACAATAACAAAAACAAAAATGAAGAATATGTTACTTTCAAAAAAAAAAAAAAATGAAGAAGATGTTTGGAATGTTCGAGAATAAATCAAAATAAAATTTAAACCAAATATTAAATTCATCAAAATTTATTAACTACTATCTAAAGCTAGTAGTAGTTAATCTTATCTTCGAACTTGATTGCTTGCGAAATGCAAGTTATTTGCTCTCTAAATTTAGGCTAAAAGAAGGCAAAAAGAGCCTCCAATGCGTTATCTTAGCGCATAAAGAAAAGGGAAAGTGCTTTGATATAAGCGGAAGGCTATATTCATGTATGCGATAAGAAATTATAAACACAGAGCGCTAACGCAACTAATCTAGACATTTTAAGTATCTTTTCAGCGCAGGCACACTTTCCAAGATGATCAAAAGACAGAAAAAGATGGGTGAAAAGACAAGCGCCGGCGCAGCCAGAACAAGTAGGCGATAAAGGAGGTTTAAAAAAAAGGGCGCAAGTGGCCTAAATGATGATTAAGCGCTAACACAACTAGCGCAAGTGGACTTGCAAGTTGACCGTTGGTGACTTGAAGAAGTTTGCTCCCTTCACCTACAAATAGAACTCACTTATTCAAAGGAAGGGGTGAAAAAAAAAAAGAGAGAAGAAGAACCTTAGAGAGGAGCTGCAGAAATCTGTAAAGGGGAATACACTTGTGTGTAATGTATCTTTTCAAGTTTATCTGTTTTGCTTTCATGAGCTAAATCTTCTCATCAAGGCTTAGCGCAAGCGCCTCATGCGGCGATAAGCATCACTTGTAAGCGCATATTTTTGGTTTAATAGAAACATTGTTTGCTTTCTACTTAGTTGTGTTAATTCTTTTATTGCTAAGTCCCGCTTGGCCAGCTGGATGCGGTATCAGTATGATAAATCCTTGACAGGTAATGCATATGGATCTCTCTAAGTAGAACAAGCTAGCGCATGTCATGTAGTCTTGGTAACATTTAATTATTACCACTAAAAGATATTGCATGTTTGAGCTTTGATTGCATTATGACTAACGCAATCTCTTGTAAGTATTACGAGAACTGAACTTCACGCATAAAACTAGGCACAGAGTCATTTGCTAGTTTATTGTTAAAATGCATAAGGAGTACCGGAAGGAAAACTTAATGATTTCTTCTCTTACTTAAGCATTAATGGATCAATTAATCAAAAATTAGCGCTACAAGTCTCTTATGAACGCATGAGGTTGCCACCGCAAGAGCCTTGATATTTTTCGCATTTAAGTCCTTTCCTCAAATTACATTATTTTCTTTCCGCAATACATTTATTTTTTCAAACTACACCACTCAATTTTCTTTACTTACCTAGCATGTGGAGAGATTAAATCAGTGAATCTATAATTTTTTTTTGTTCCATTTCCCTGTGGATTCGACCCTGAACTCATCAGGTACTACTTGATTTAATCAGTTTATACTTGGATTGATTTAAATCTGCGACTATTCTACCAACTATACTGATATTCAATCAGAACTCCAGTAAAGTCATCCTTATTTTTCAGGGAATGGCTACCATCACCATTTCCGCCATACTTCCAAAGCTTCACCCACCACCCTGCCCAACCCAACTCAACTGCATACAAGCCTCCGGCACGCAACTGACGGTCCTCTACCTCTCCCTCCTCCTCACATCCCTCGGCCCGGGCGGCATTCGGCCCTGCGTCGTCACCTTCGCCGCCGACCAGTTCGACATGTCGAAGGTCGGCGTCGCAGGCCGAACGTGGAACTTCTTCAACTGGTACTATTTCTGCATGGGACTGGCGACTCTCACGGCCCTCACCGTGGTGGTCTATATCCAGGACAACGTGGGCTGGGGCTGGGGCTTCGGCCTTCCCACCATTGCCATGGCCTTGTCTGTTGTGGCCTTCGTCGTGGGCTCGCCCCTTTACAACAAACTGAAGCCCAGTGGCAGCCCCTTGGTTCGATTGGCTCAGGTCGTCGTGGCTGCGGTCAGGAAGCGAAAGGCTGTTCTGCCTCATGACTCCAAGCTTTTGTATAGGAACCATAAGCTTGATGCTGCCATTGCCGTTCAGGGAAGGCTCGTCCACACGGATCAATTCAAGTAAGCCATGATAACTAAAGGTGTTCAATTATAAAATTTTGATCTTGTGGGTGTTGGATTTTGGTTTTGGTAAGTTGTTAGGAGTTAATACAATTAGATATTTGACATGAGAATTTGGACTAGTTAGTGATGATTTGATAACAAAGGCGAGTATAAAACACATTTTAAACTGAATTTTAAGGACTTGACACTAGACAACCATTATTTTGTCTATTAGCATATGTTCACATTTTTTAAAACCAAAATTGGAAGCTTACCTATCTCGGAAAATTATTTGAGACTAGATAGACATCAACATTAAAGTGTTAAAATTCTTTTTAATGATTAAATTACAAGTATTATGGACATCTCAAGTTTCAAGATTGTGTCTATTTAGTCTCTATACCATATATGTTTTTAATAAGTCTCAATATCTTCAAGATTGTCGCTAAGCTTTCAACAAAATATCACATGGGTGGTCCTTATATTGGTTTGATCCAATTTTTTAACTAAAAAAAGAGAAGTTTCTACTACATATTTCATTAAAATCTTGACAATTAAGAAAGAGAAAAGTAGAAAACGAGAAAATTTATTTGTCTCTAATAGTTGTCAACATTTTAATGGATTCCAGTATGCAACCACGTTTTTTTCTTATTTAAGATTTACATGACAACTTGACAAACTAATTTTGGACAAATTTAATTTCTTTTAAGTTCAACAATGAGATTCGAATCTCTAACCTCTTGGTCAAAGGTACATGTCAACTGTTGTTAAACTATACTCACTTTGACAAATTTAGTTGATTAGTAGCGTAAAGAATTAACAACAATATAACTTACATGACATTGTTCAACCTTTTCAAATTCGAAAGACTAAAGAAACACAATATATAGAGAAATTTTTAAGTGCTCCCGATATAATGAGGCAGATAGTTTTGCACTGTTGGATGTAAGAGAGAAATTGTAAAAGTATCTCGATTTTGTATCCAACGGTTATTAGGGATAAACAAATTTGAAAATGTGACGGTTTCAAAGATAGAGGCTCATGAAATTTAGTCCAACTTAATCATAACTTCTCTTTGCACGACATTTTCAGGTGGTTGGACAAGGCCGCCGTCATAACAAGCCCAGATGAAACAAACTCAAGAAACGACGAGGCACCGAACCTCTGGCGGCTGGCCACTGTGCACCGAATCGAAGAGCTAAAATCCATAATCCGAATGCTTCCAATATGGGCGGCCGGAATCCTCCTGGTGACCGCCTCCTCCCACCAACACAGCTTCACAATCCAACAGGCCCGCACAATGAACCGCCACCTGACACCCACCTTCCAAATCCCGCCGGCCAGTCTCTCCATCTTCGGCATCCTCTCCATGCTCACCGGCCTCGTCCTCTACGAGCGCCTCCTCGTCCCCTTCGCCAAACGGTTCACCAAAAACCCCTCCGGCATCACCTGCCTCCAGCGCATGGGCGTCGGCTTCGCCATCAACATCCTCGCCACGCTCGTCTCCTCCGCCGTCGAGATCCGGAGAAAGAAAGTCGCGGCCAGACATGGCCTGCTGGACGCCTCGACAGCGACGATCCCGTTCAGCGTGTTCTGGCTGGTGCCGCAGTTCTGGCTGCATGGGATCGCAGAGGTTTTCATGTCGGTGGGGCATTTGGAGTTCATGTACGACCAGTCGCCGGAGAGCTTGAGGAGCACGGCGACGGTGCTGTACTGGCTGGCGATATCGGTGGGGAATTACGTGGGGACTTTGATGGTGTATCTTGTGCATAAGTACAGTGGGAAGGAGCATAATTGGTTGCCGGATAGGAATCTGAATAGAGGGAGGTTGGAGTATTATTATTGGCTTGTGAGTGGGATTCAAGCTTTGAATCTTGTGTATTATGTGGTTTGTGCTTCGTTTTATACTTGTAAGCCTTTGGAAGAGGAGACTGAGGAGGGTGCTGAAGAGAAACAGCTCGCTTCTTCTTCCAGCTTCTCTAAGGATAACAATGGGTGTCAACAGACAAACACAGCTTAA

mRNA sequence

ATGACAGGGAACGAAGTTTGTGATAGATTTGCAAGCACGGGTTTCCATGCGAACATAATAACATATTTGACACAAGATCTGAACATGGCTCTTGTTCCAGCCTCCAACATTCTCACCAACTTCGCTGCTACTTCTAGCTTCACTTCTCTCATCGGCGCTCTCATCGCCGACTCCTTCGCCGGCCGCTTCTCGACCATCATCGTTGCCTCCATCATCTATGAGCTCGTATATATACCCCTCTCTCTCGACACTGTTAAATTTCATTTTAGCACACTTTCCAAGATGATCAAAAGACAGAAAAAGATGGGTGAAAAGACAAGCGCCGGCGCAGCCAGAACAACTAAATCTTCTCATCAAGGCTTAGCGCAAGCGCCTCATGCGGCGATAAGCATCACTTTCCCGCTTGGCCAGCTGGATGCGGTATCAAACTCCAGTAAAGTCATCCTTATTTTTCAGGGAATGGCTACCATCACCATTTCCGCCATACTTCCAAAGCTTCACCCACCACCCTGCCCAACCCAACTCAACTGCATACAAGCCTCCGGCACGCAACTGACGGTCCTCTACCTCTCCCTCCTCCTCACATCCCTCGGCCCGGGCGGCATTCGGCCCTGCGTCGTCACCTTCGCCGCCGACCAGTTCGACATGTCGAAGGTCGGCGTCGCAGGCCGAACGTGGAACTTCTTCAACTGGTACTATTTCTGCATGGGACTGGCGACTCTCACGGCCCTCACCGTGGTGGTCTATATCCAGGACAACGTGGGCTGGGGCTGGGGCTTCGGCCTTCCCACCATTGCCATGGCCTTGTCTGTTGTGGCCTTCGTCGTGGGCTCGCCCCTTTACAACAAACTGAAGCCCAGTGGCAGCCCCTTGGTTCGATTGGCTCAGGTCGTCGTGGCTGCGGTCAGGAAGCGAAAGGCTGTTCTGCCTCATGACTCCAAGCTTTTGTATAGGAACCATAAGCTTGATGCTGCCATTGCCGTTCAGGGAAGGCTCGTCCACACGGATCAATTCAAGTGGTTGGACAAGGCCGCCGTCATAACAAGCCCAGATGAAACAAACTCAAGAAACGACGAGGCACCGAACCTCTGGCGGCTGGCCACTGTGCACCGAATCGAAGAGCTAAAATCCATAATCCGAATGCTTCCAATATGGGCGGCCGGAATCCTCCTGGTGACCGCCTCCTCCCACCAACACAGCTTCACAATCCAACAGGCCCGCACAATGAACCGCCACCTGACACCCACCTTCCAAATCCCGCCGGCCAGTCTCTCCATCTTCGGCATCCTCTCCATGCTCACCGGCCTCGTCCTCTACGAGCGCCTCCTCGTCCCCTTCGCCAAACGGTTCACCAAAAACCCCTCCGGCATCACCTGCCTCCAGCGCATGGGCGTCGGCTTCGCCATCAACATCCTCGCCACGCTCGTCTCCTCCGCCGTCGAGATCCGGAGAAAGAAAGTCGCGGCCAGACATGGCCTGCTGGACGCCTCGACAGCGACGATCCCGTTCAGCGTGTTCTGGCTGGTGCCGCAGTTCTGGCTGCATGGGATCGCAGAGGTTTTCATGTCGGTGGGGCATTTGGAGTTCATGTACGACCAGTCGCCGGAGAGCTTGAGGAGCACGGCGACGGTGCTGTACTGGCTGGCGATATCGGTGGGGAATTACGTGGGGACTTTGATGGTGTATCTTGTGCATAAGTACAGTGGGAAGGAGCATAATTGGTTGCCGGATAGGAATCTGAATAGAGGGAGGTTGGAGTATTATTATTGGCTTGTGAGTGGGATTCAAGCTTTGAATCTTGTGTATTATGTGGTTTGTGCTTCGTTTTATACTTGTAAGCCTTTGGAAGAGGAGACTGAGGAGGGTGCTGAAGAGAAACAGCTCGCTTCTTCTTCCAGCTTCTCTAAGGATAACAATGGGTGTCAACAGACAAACACAGCTTAA

Coding sequence (CDS)

ATGACAGGGAACGAAGTTTGTGATAGATTTGCAAGCACGGGTTTCCATGCGAACATAATAACATATTTGACACAAGATCTGAACATGGCTCTTGTTCCAGCCTCCAACATTCTCACCAACTTCGCTGCTACTTCTAGCTTCACTTCTCTCATCGGCGCTCTCATCGCCGACTCCTTCGCCGGCCGCTTCTCGACCATCATCGTTGCCTCCATCATCTATGAGCTCGTATATATACCCCTCTCTCTCGACACTGTTAAATTTCATTTTAGCACACTTTCCAAGATGATCAAAAGACAGAAAAAGATGGGTGAAAAGACAAGCGCCGGCGCAGCCAGAACAACTAAATCTTCTCATCAAGGCTTAGCGCAAGCGCCTCATGCGGCGATAAGCATCACTTTCCCGCTTGGCCAGCTGGATGCGGTATCAAACTCCAGTAAAGTCATCCTTATTTTTCAGGGAATGGCTACCATCACCATTTCCGCCATACTTCCAAAGCTTCACCCACCACCCTGCCCAACCCAACTCAACTGCATACAAGCCTCCGGCACGCAACTGACGGTCCTCTACCTCTCCCTCCTCCTCACATCCCTCGGCCCGGGCGGCATTCGGCCCTGCGTCGTCACCTTCGCCGCCGACCAGTTCGACATGTCGAAGGTCGGCGTCGCAGGCCGAACGTGGAACTTCTTCAACTGGTACTATTTCTGCATGGGACTGGCGACTCTCACGGCCCTCACCGTGGTGGTCTATATCCAGGACAACGTGGGCTGGGGCTGGGGCTTCGGCCTTCCCACCATTGCCATGGCCTTGTCTGTTGTGGCCTTCGTCGTGGGCTCGCCCCTTTACAACAAACTGAAGCCCAGTGGCAGCCCCTTGGTTCGATTGGCTCAGGTCGTCGTGGCTGCGGTCAGGAAGCGAAAGGCTGTTCTGCCTCATGACTCCAAGCTTTTGTATAGGAACCATAAGCTTGATGCTGCCATTGCCGTTCAGGGAAGGCTCGTCCACACGGATCAATTCAAGTGGTTGGACAAGGCCGCCGTCATAACAAGCCCAGATGAAACAAACTCAAGAAACGACGAGGCACCGAACCTCTGGCGGCTGGCCACTGTGCACCGAATCGAAGAGCTAAAATCCATAATCCGAATGCTTCCAATATGGGCGGCCGGAATCCTCCTGGTGACCGCCTCCTCCCACCAACACAGCTTCACAATCCAACAGGCCCGCACAATGAACCGCCACCTGACACCCACCTTCCAAATCCCGCCGGCCAGTCTCTCCATCTTCGGCATCCTCTCCATGCTCACCGGCCTCGTCCTCTACGAGCGCCTCCTCGTCCCCTTCGCCAAACGGTTCACCAAAAACCCCTCCGGCATCACCTGCCTCCAGCGCATGGGCGTCGGCTTCGCCATCAACATCCTCGCCACGCTCGTCTCCTCCGCCGTCGAGATCCGGAGAAAGAAAGTCGCGGCCAGACATGGCCTGCTGGACGCCTCGACAGCGACGATCCCGTTCAGCGTGTTCTGGCTGGTGCCGCAGTTCTGGCTGCATGGGATCGCAGAGGTTTTCATGTCGGTGGGGCATTTGGAGTTCATGTACGACCAGTCGCCGGAGAGCTTGAGGAGCACGGCGACGGTGCTGTACTGGCTGGCGATATCGGTGGGGAATTACGTGGGGACTTTGATGGTGTATCTTGTGCATAAGTACAGTGGGAAGGAGCATAATTGGTTGCCGGATAGGAATCTGAATAGAGGGAGGTTGGAGTATTATTATTGGCTTGTGAGTGGGATTCAAGCTTTGAATCTTGTGTATTATGTGGTTTGTGCTTCGTTTTATACTTGTAAGCCTTTGGAAGAGGAGACTGAGGAGGGTGCTGAAGAGAAACAGCTCGCTTCTTCTTCCAGCTTCTCTAAGGATAACAATGGGTGTCAACAGACAAACACAGCTTAA

Protein sequence

MTGNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGRFSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLAQAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASGTQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAVRKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPASLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEIRRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVYYVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTNTA
Homology
BLAST of Spg008925 vs. NCBI nr
Match: KAG6596175.1 (Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 959.9 bits (2480), Expect = 1.1e-275
Identity = 506/645 (78.45%), Postives = 527/645 (81.71%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE+CDRFASTGFHANIITYLTQDLNM LVPASNILTNFAATSSFT+LIGALIADSFAGR
Sbjct: 46  GNEICDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGR 105

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           F TI  ASIIYEL                                               
Sbjct: 106 FWTITFASIIYEL----------------------------------------------- 165

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GMAT+TISAI+P+LHPPPCPTQLNCIQAS 
Sbjct: 166 ------------------------------GMATVTISAIIPRLHPPPCPTQLNCIQAST 225

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           TQLTVLYL+LLLTSLG GGIRPCVV FAADQFDM+KVGVAGRTWNFFNWYYFCMGLATLT
Sbjct: 226 TQLTVLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGLATLT 285

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV
Sbjct: 286 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 345

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           +KRKA LP D KLLYRNHKLDAAIA+QGRLVHTDQFKWLDKAAVIT+ + T+    + PN
Sbjct: 346 KKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTDQFKWLDKAAVITTDESTS----DPPN 405

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
           LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFT+QQARTMNRHLTPTF+IPPA
Sbjct: 406 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTVQQARTMNRHLTPTFEIPPA 465

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLSIFGILSMLTGLVLYERLLVPFA+RFTKNPSGITCLQRMGVGFAINILATLVSS +EI
Sbjct: 466 SLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGITCLQRMGVGFAINILATLVSSLIEI 525

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
           RRKKVAA H LLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA
Sbjct: 526 RRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 585

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVY 602
           T LYWLAIS+GNYVGTLMV LVHKYSGKEHNWLPDRNLNRGRLE YYWLVSGIQ LNLVY
Sbjct: 586 TALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDRNLNRGRLERYYWLVSGIQVLNLVY 603

Query: 603 YVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTNTA 648
           YVVCA FYT KPLEEE ++  E     SSS+ SKD  GCQ T TA
Sbjct: 646 YVVCAWFYTYKPLEEEKDDEGE----LSSSTLSKD--GCQDTKTA 603

BLAST of Spg008925 vs. NCBI nr
Match: KAG7027713.1 (Protein NRT1/ PTR FAMILY 3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 959.9 bits (2480), Expect = 1.1e-275
Identity = 506/645 (78.45%), Postives = 527/645 (81.71%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE+CDRFASTGFHANIITYLTQDLNM LVPASNILTNFAATSSFT+LIGALIADSFAGR
Sbjct: 42  GNEICDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGR 101

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           F TI  ASIIYEL                                               
Sbjct: 102 FWTITFASIIYEL----------------------------------------------- 161

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GMAT+TISAI+P+LHPPPCPTQLNCIQAS 
Sbjct: 162 ------------------------------GMATVTISAIIPRLHPPPCPTQLNCIQAST 221

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           TQLTVLYL+LLLTSLG GGIRPCVV FAADQFDM+KVGVAGRTWNFFNWYYFCMGLATLT
Sbjct: 222 TQLTVLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGLATLT 281

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV
Sbjct: 282 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 341

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           +KRKA LP D KLLYRNHKLDAAIA+QGRLVHTDQFKWLDKAAVIT+ + T+    + PN
Sbjct: 342 KKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTDQFKWLDKAAVITTDESTS----DPPN 401

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
           LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFT+QQARTMNRHLTPTF+IPPA
Sbjct: 402 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTVQQARTMNRHLTPTFEIPPA 461

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLSIFGILSMLTGLVLYERLLVPFA+RFTKNPSGITCLQRMGVGFAINILATLVSS +EI
Sbjct: 462 SLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGITCLQRMGVGFAINILATLVSSLIEI 521

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
           RRKKVAA H LLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA
Sbjct: 522 RRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 581

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVY 602
           T LYWLAIS+GNYVGTLMV LVHKYSGKEHNWLPDRNLNRGRLE YYWLVSGIQ LNLVY
Sbjct: 582 TALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDRNLNRGRLERYYWLVSGIQVLNLVY 599

Query: 603 YVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTNTA 648
           YVVCA FYT KPLEEE ++  E     SSS+ SKD  GCQ T TA
Sbjct: 642 YVVCAWFYTYKPLEEEKDDEGE----LSSSTLSKD--GCQDTKTA 599

BLAST of Spg008925 vs. NCBI nr
Match: XP_022971207.1 (protein NRT1/ PTR FAMILY 3.1-like [Cucurbita maxima])

HSP 1 Score: 958.4 bits (2476), Expect = 3.2e-275
Identity = 505/645 (78.29%), Postives = 526/645 (81.55%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE+CDRFASTGFHANIITYLTQDLNM LVPASNILTNFAATSSFT+LIGALIADSFAGR
Sbjct: 45  GNEICDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGR 104

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           F TI  ASIIYEL                                               
Sbjct: 105 FWTITFASIIYEL----------------------------------------------- 164

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GMATITISAI+P+LHPPPCPTQLNCIQAS 
Sbjct: 165 ------------------------------GMATITISAIIPRLHPPPCPTQLNCIQASS 224

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           TQLTV YL+LLLTSLG GGIRPCVV FAADQFDM+KVGVAGRTWNFFNWYYFCMGLATLT
Sbjct: 225 TQLTVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGLATLT 284

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV
Sbjct: 285 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 344

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           +KRKA LP D KLLYRNHKLDAAIA+QGRLVHTDQFKWLDKAAVIT+ + T+    + PN
Sbjct: 345 KKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTDQFKWLDKAAVITTDESTS----DPPN 404

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
           LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFT+QQARTMNRHLTPTF+IPPA
Sbjct: 405 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTVQQARTMNRHLTPTFEIPPA 464

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLSIFGILSMLTGLVLYERLLVPFA+RFTKNPSGITCLQRMGVGFAINILATLVSS +EI
Sbjct: 465 SLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGITCLQRMGVGFAINILATLVSSLIEI 524

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
           RRKKVAA H LLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA
Sbjct: 525 RRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 584

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVY 602
           T LYWLAIS+GNYVGTLMV LVHKYSGKEHNWLPDRNLNRGRLE YYWLVSGIQ LNLVY
Sbjct: 585 TALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDRNLNRGRLERYYWLVSGIQVLNLVY 602

Query: 603 YVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTNTA 648
           YVVCA FYT KPLEEE ++  E     SSS+ SKD  GC+ T TA
Sbjct: 645 YVVCAWFYTYKPLEEEKDDEGE----PSSSTLSKD--GCEDTKTA 602

BLAST of Spg008925 vs. NCBI nr
Match: XP_023540126.1 (protein NRT1/ PTR FAMILY 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 958.0 bits (2475), Expect = 4.2e-275
Identity = 506/645 (78.45%), Postives = 524/645 (81.24%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE+CDRFASTGFHANIITYLTQDLNM LVPASNILTNFAATSSFT+LIGALIADSFAGR
Sbjct: 45  GNEICDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGR 104

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           F TI  ASIIYEL                                               
Sbjct: 105 FWTITFASIIYEL----------------------------------------------- 164

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GMATITISAI+P LHPPPCPTQLNCIQAS 
Sbjct: 165 ------------------------------GMATITISAIIPSLHPPPCPTQLNCIQASS 224

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           TQLTVLYLSLLLTSLG GGIRPCVV FAADQFDM+KVGVAGRTWNFFNWYYFCMGLATLT
Sbjct: 225 TQLTVLYLSLLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGLATLT 284

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRL QVVVAAV
Sbjct: 285 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLTQVVVAAV 344

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           +KRKA LP D KLLYRNHKLDAAIA+QGRLVHT QFKWLDKAAVIT+ + T+    + PN
Sbjct: 345 KKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTHQFKWLDKAAVITTDESTS----DPPN 404

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
           LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFT+QQARTMNRHLTPTF+IPPA
Sbjct: 405 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTVQQARTMNRHLTPTFEIPPA 464

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLSIFGILSMLTGLVLYERLLVPFA+RFTKNPSGITCLQRMGVGFAINILATLVSS +EI
Sbjct: 465 SLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGITCLQRMGVGFAINILATLVSSLIEI 524

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
           RRKKVAA H LLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA
Sbjct: 525 RRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 584

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVY 602
           T LYWLAIS+GNYVGTLMV LVHKYSGKEHNWLPDRNLNRGRLE YYWLVSGIQ LNLVY
Sbjct: 585 TALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDRNLNRGRLERYYWLVSGIQVLNLVY 602

Query: 603 YVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTNTA 648
           YVVCA FYT KPLEEE ++  E     SSS+ SKD  GCQ T TA
Sbjct: 645 YVVCARFYTYKPLEEEKDDEGE----PSSSTLSKD--GCQDTKTA 602

BLAST of Spg008925 vs. NCBI nr
Match: XP_022941237.1 (protein NRT1/ PTR FAMILY 3.1-like [Cucurbita moschata])

HSP 1 Score: 956.1 bits (2470), Expect = 1.6e-274
Identity = 505/645 (78.29%), Postives = 526/645 (81.55%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE+CDRFASTGFHANIITYLTQDLNM LVPASNILTNFAATSSFT+LIGALIADSFAGR
Sbjct: 46  GNEICDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGR 105

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           F TI  ASIIYEL                                               
Sbjct: 106 FWTITFASIIYEL----------------------------------------------- 165

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GMATITISAI+P+LHPPPCPTQLNCIQAS 
Sbjct: 166 ------------------------------GMATITISAIIPRLHPPPCPTQLNCIQAST 225

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           TQLTVLYL+LLLTSLG GGIRPCVV FAADQFDM+KVGVAGRTWNFFNWYYFCMGLATLT
Sbjct: 226 TQLTVLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGLATLT 285

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVV AV
Sbjct: 286 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVTAV 345

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           +KRKA LP D KLLYRNHKLDAAIA+QGRLVHT+QFKWLDKAAVIT+ + T+    + PN
Sbjct: 346 KKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTNQFKWLDKAAVITTDESTS----DPPN 405

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
           LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFT+QQARTMNRHLTPTF+IPPA
Sbjct: 406 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTVQQARTMNRHLTPTFEIPPA 465

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLSIFGILSMLTGLVLYERLLVPFA+RFTKNPSGITCLQRMGVGFAINILATLVSS +EI
Sbjct: 466 SLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGITCLQRMGVGFAINILATLVSSLIEI 525

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
           RRKKVAA H LLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA
Sbjct: 526 RRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 585

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVY 602
           T LYWLAIS+GNYVGTLMV LVHKYSGKEHNWLPDRNLNRGRLE YYWLVSGIQ LNLVY
Sbjct: 586 TALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDRNLNRGRLERYYWLVSGIQVLNLVY 603

Query: 603 YVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTNTA 648
           YVVCA FYT KPLEEE ++  E     SSS+ SKD  GCQ T TA
Sbjct: 646 YVVCAWFYTYKPLEEEKDDEGE----PSSSTLSKD--GCQGTKTA 603

BLAST of Spg008925 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 6.1e-176
Identity = 325/636 (51.10%), Postives = 417/636 (65.57%), Query Frame = 0

Query: 4   NEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGRF 63
           NE+C++ A  GFHAN+I+YLT  L++ L  A+N LTNFA TSS T L+GA IADSFAGRF
Sbjct: 36  NEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRF 95

Query: 64  STIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLAQ 123
            TI  ASIIY++                                                
Sbjct: 96  WTITFASIIYQI------------------------------------------------ 155

Query: 124 APHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASGT 183
                                        GM  +TISAI+P L PPPC  +  C+ A   
Sbjct: 156 -----------------------------GMTLLTISAIIPTLRPPPCKGEEVCVVADTA 215

Query: 184 QLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLTA 243
           QL++LY++LLL +LG GGIRPCVV F ADQFD S      +TWN+FNWYYFCMG A L A
Sbjct: 216 QLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLA 275

Query: 244 LTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAVR 303
           +TV+V+IQDNVGWG G G+PT+AM LSV+AFV G  LY  L P+GSP  RL QV VAA R
Sbjct: 276 VTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFR 335

Query: 304 KRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPNL 363
           KRK  +  D  LLY N ++DA I++ G+L HT    +LDKAA++T  +E N +  + PN 
Sbjct: 336 KRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVT--EEDNLKPGQIPNH 395

Query: 364 WRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPAS 423
           WRL+TVHR+EELKS+IRM PI A+GILL+TA + Q +F++QQA+TMNRHLT +FQIP  S
Sbjct: 396 WRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGS 455

Query: 424 LSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEIR 483
           +S+F  ++MLT ++ Y+R+ V  A++FT    GIT L RMG+GF I+I+ATLV+  VE++
Sbjct: 456 MSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVK 515

Query: 484 RKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTAT 543
           RK VA  HGLLD     +P S  WL+PQ+ LHG+AE FMS+GHLEF YDQ+PES+RSTAT
Sbjct: 516 RKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTAT 575

Query: 544 VLYWLAISVGNYVGTLMVYLVHKYSGKE--HNWLPDRNLNRGRLEYYYWLVSGIQALNLV 603
            L+W+AIS+GNYV TL+V LVHK+S K    NWLPD NLNRGRLEY+YWL++ +QA+NLV
Sbjct: 576 ALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLV 592

Query: 604 YYVVCASFYTCKPLE-----EETEEGAEEKQLASSS 633
           YY+ CA  YT KP++     E++    EE QL++ S
Sbjct: 636 YYLWCAKIYTYKPVQVHHSKEDSSPVKEELQLSNRS 592

BLAST of Spg008925 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 2.9e-109
Identity = 227/613 (37.03%), Postives = 334/613 (54.49%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           G E C+R A  G   N+I YL + +NM  V AS  ++N++ T   T LIGA IAD++ GR
Sbjct: 37  GTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGR 96

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           + TI                                                        
Sbjct: 97  YWTI-------------------------------------------------------- 156

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                +S V++   GM  +TISA +P L  P C  +     A+ 
Sbjct: 157 ---------------------ASFVVIYIAGMTLLTISASVPGL-TPTCSGE--TCHATA 216

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
            Q  + +++L L +LG GGI+PCV +F ADQFD +         +FFNW+YF + +  + 
Sbjct: 217 GQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGAMI 276

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           A +V+V+IQ NVGWGWG G+PT+AMA++VV F  GS  Y   KP GSPL R+ QV+VA+ 
Sbjct: 277 ASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVASC 336

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           RK K  +P D  LLY N   +++I    +L HT    + DKAAV T  D   +    +  
Sbjct: 337 RKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAAKSSS-- 396

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
            W+L TV ++EELK++IR+LPIWA GI+  +  S   +  + Q  T+++H+ P F+IP A
Sbjct: 397 -WKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSA 456

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLS+F  LS+L    +Y++L+VPFA+++T +  G T LQR+G+G  I+I + + +  +E+
Sbjct: 457 SLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEV 516

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
            R      H L +    TIP ++FW VPQ++L G AEVF  +G LEF YDQ+P+++RS  
Sbjct: 517 ARLNYVQTHNLYNEE--TIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLC 563

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKY--SGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNL 602
           + L   AI+ GNY+ T +V LV K   SG    W+  +NLN G L+Y++WL++G+  LN 
Sbjct: 577 SALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIA-KNLNNGHLDYFFWLLAGLSFLNF 563

Query: 603 VYYVVCASFYTCK 614
           + Y+  A +YT K
Sbjct: 637 LVYLWIAKWYTYK 563

BLAST of Spg008925 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 5.1e-106
Identity = 220/613 (35.89%), Postives = 326/613 (53.18%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE C+R A  G   N++ YL   LN     A+N +TN++ T   T LIGA IAD++ GR
Sbjct: 36  GNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGR 95

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           + TI     IY                                                 
Sbjct: 96  YWTIATFVFIY------------------------------------------------- 155

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GM  +T+SA +P L P  C    +    + 
Sbjct: 156 ----------------------------VSGMTLLTLSASVPGLKPGNC--NADTCHPNS 215

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           +Q  V +++L + +LG GGI+PCV +F ADQFD +      +  +FFNW+YF + +  L 
Sbjct: 216 SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALI 275

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           A TV+V+IQ NVGWGWGFG+PT+AM ++V  F  GS  Y   +P GSPL R+ QV+VAA 
Sbjct: 276 AATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAF 335

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           RK    +P D  LL+     ++ I    +LVHTD  K+ DKAAV     +++S  D   N
Sbjct: 336 RKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV---ESQSDSIKDGEVN 395

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
            WRL +V ++EELKSII +LP+WA GI+  T  S   +  + Q  TM++H+   F+IP A
Sbjct: 396 PWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSA 455

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLS+F  +S+L    +Y++ ++P A++FT+N  G T LQRMG+G  ++I A + +  +E+
Sbjct: 456 SLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEV 515

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
            R      H   D     I  S+FW +PQ+ L G AEVF  +G LEF YDQ+P+++RS  
Sbjct: 516 VRLDYVKTHNAYDQK--QIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLC 563

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGK--EHNWLPDRNLNRGRLEYYYWLVSGIQALNL 602
           + L    +++GNY+ T++V +V K + K  +  W+PD NLNRG L+Y+++L++ +  LN 
Sbjct: 576 SALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYFFYLLATLSFLNF 563

Query: 603 VYYVVCASFYTCK 614
           + Y+  +  Y  K
Sbjct: 636 LVYLWISKRYKYK 563

BLAST of Spg008925 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 5.1e-106
Identity = 224/613 (36.54%), Postives = 337/613 (54.98%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE C+R A  G   N+ITYLT  L+   V A+  +T +  T   T LIGA++AD++ GR
Sbjct: 53  GNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGR 112

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           + TI   S IY                                                 
Sbjct: 113 YWTIACFSGIY------------------------------------------------- 172

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                       F GM+ +T+SA +P L P  C     C  A+ 
Sbjct: 173 ----------------------------FIGMSALTLSASVPALKPAECIGDF-CPSATP 232

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
            Q  + +  L L +LG GGI+PCV +F ADQFD +      R  +FFNW+YF + +  L 
Sbjct: 233 AQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALV 292

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           + +++V+IQ+N GWG GFG+PT+ M L++ +F  G+PLY   KP GSP+ R++QVVVA+ 
Sbjct: 293 SSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASF 352

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           RK    +P D+ LLY     ++AIA   ++ HTD  ++LDKAAVI+   E  S++ +  N
Sbjct: 353 RKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS---EEESKSGDYSN 412

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
            WRL TV ++EELK +IRM PIWA+GI+     +   +  +QQ R MN  +  +FQ+PPA
Sbjct: 413 SWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPA 472

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           +L  F   S++  + LY+R +VP A++FT    G T +QRMG+G  +++L    ++ VEI
Sbjct: 473 ALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEI 532

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
            R  +A   GL++ S A +P SV W +PQ+++ G AEVF  +G LEF YDQSP+++RS  
Sbjct: 533 IRLHMANDLGLVE-SGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLC 581

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGK--EHNWLPDRNLNRGRLEYYYWLVSGIQALNL 602
           + L  L  ++GNY+ +L++ LV  ++ +  +  W+ D NLN G L+Y++WL++G+  +N+
Sbjct: 593 SALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISD-NLNSGHLDYFFWLLAGLSLVNM 581

Query: 603 VYYVVCASFYTCK 614
             Y   A+ Y  K
Sbjct: 653 AVYFFSAARYKQK 581

BLAST of Spg008925 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.2e-104
Identity = 226/618 (36.57%), Postives = 339/618 (54.85%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE C+R A  G   N+ITY T +L+ + V A++ +  +  T   T LIGA+IADS+ GR
Sbjct: 64  GNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYITPLIGAVIADSYWGR 123

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           + TI   S IY                                                 
Sbjct: 124 YWTIASFSAIY------------------------------------------------- 183

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCP--TQLNCIQA 182
                                       F GMA +T+SA LP L P  C       C  A
Sbjct: 184 ----------------------------FIGMALLTLSASLPVLKPAACAGVAAALCSPA 243

Query: 183 SGTQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLAT 242
           +  Q  V +  L L +LG GGI+PCV +F ADQFD +      R  +FFNW+YF + + +
Sbjct: 244 TTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGS 303

Query: 243 LTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVA 302
             + T++V++Q+NVGWG GF +PT+ M +S+ +F +G+PLY   KP GSP+ R+ QV+VA
Sbjct: 304 FISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVA 363

Query: 303 AVRKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEA 362
           A RK K  LP D   LY   + ++ IA   ++ HTD +K+LDKAAVI+   E  S++   
Sbjct: 364 AYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVIS---EYESKSGAF 423

Query: 363 PNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIP 422
            N W+L TV ++EE+K++IRM PIWA+GI+     S   +  +QQ R+MNR +  +F+IP
Sbjct: 424 SNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNR-IIRSFEIP 483

Query: 423 PASLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAV 482
           PAS  +F  L +L  + +Y+R LVPF +RFT  P G+T LQRMG+G  +++L+   ++ V
Sbjct: 484 PASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIV 543

Query: 483 EIRRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRS 542
           E  R ++A            +  S+FW +PQ+ L GIAEVF  +G +EF YD+SP+++RS
Sbjct: 544 ETVRLQLAQDF---------VAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRS 590

Query: 543 TATVLYWLAISVGNYVGTLMVYLVHKYS--GKEHNWLPDRNLNRGRLEYYYWLVSGIQAL 602
             + L  L  +VG+Y+ +L++ LV  ++  G +  W+PD +LN+G L+Y++WL+  +  +
Sbjct: 604 VCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPD-DLNKGHLDYFFWLLVSLGLV 590

Query: 603 NL-VYYVVCASFYTCKPL 616
           N+ VY ++C      K L
Sbjct: 664 NIPVYALICVKHTKKKAL 590

BLAST of Spg008925 vs. ExPASy TrEMBL
Match: A0A6J1I1C5 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita maxima OX=3661 GN=LOC111469999 PE=3 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.6e-275
Identity = 505/645 (78.29%), Postives = 526/645 (81.55%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE+CDRFASTGFHANIITYLTQDLNM LVPASNILTNFAATSSFT+LIGALIADSFAGR
Sbjct: 45  GNEICDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGR 104

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           F TI  ASIIYEL                                               
Sbjct: 105 FWTITFASIIYEL----------------------------------------------- 164

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GMATITISAI+P+LHPPPCPTQLNCIQAS 
Sbjct: 165 ------------------------------GMATITISAIIPRLHPPPCPTQLNCIQASS 224

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           TQLTV YL+LLLTSLG GGIRPCVV FAADQFDM+KVGVAGRTWNFFNWYYFCMGLATLT
Sbjct: 225 TQLTVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGLATLT 284

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV
Sbjct: 285 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 344

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           +KRKA LP D KLLYRNHKLDAAIA+QGRLVHTDQFKWLDKAAVIT+ + T+    + PN
Sbjct: 345 KKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTDQFKWLDKAAVITTDESTS----DPPN 404

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
           LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFT+QQARTMNRHLTPTF+IPPA
Sbjct: 405 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTVQQARTMNRHLTPTFEIPPA 464

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLSIFGILSMLTGLVLYERLLVPFA+RFTKNPSGITCLQRMGVGFAINILATLVSS +EI
Sbjct: 465 SLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGITCLQRMGVGFAINILATLVSSLIEI 524

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
           RRKKVAA H LLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA
Sbjct: 525 RRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 584

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVY 602
           T LYWLAIS+GNYVGTLMV LVHKYSGKEHNWLPDRNLNRGRLE YYWLVSGIQ LNLVY
Sbjct: 585 TALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDRNLNRGRLERYYWLVSGIQVLNLVY 602

Query: 603 YVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTNTA 648
           YVVCA FYT KPLEEE ++  E     SSS+ SKD  GC+ T TA
Sbjct: 645 YVVCAWFYTYKPLEEEKDDEGE----PSSSTLSKD--GCEDTKTA 602

BLAST of Spg008925 vs. ExPASy TrEMBL
Match: A0A6J1FLW3 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita moschata OX=3662 GN=LOC111446603 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 7.7e-275
Identity = 505/645 (78.29%), Postives = 526/645 (81.55%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE+CDRFASTGFHANIITYLTQDLNM LVPASNILTNFAATSSFT+LIGALIADSFAGR
Sbjct: 46  GNEICDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGR 105

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           F TI  ASIIYEL                                               
Sbjct: 106 FWTITFASIIYEL----------------------------------------------- 165

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GMATITISAI+P+LHPPPCPTQLNCIQAS 
Sbjct: 166 ------------------------------GMATITISAIIPRLHPPPCPTQLNCIQAST 225

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           TQLTVLYL+LLLTSLG GGIRPCVV FAADQFDM+KVGVAGRTWNFFNWYYFCMGLATLT
Sbjct: 226 TQLTVLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRTWNFFNWYYFCMGLATLT 285

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVV AV
Sbjct: 286 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVTAV 345

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           +KRKA LP D KLLYRNHKLDAAIA+QGRLVHT+QFKWLDKAAVIT+ + T+    + PN
Sbjct: 346 KKRKASLPEDPKLLYRNHKLDAAIAIQGRLVHTNQFKWLDKAAVITTDESTS----DPPN 405

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
           LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFT+QQARTMNRHLTPTF+IPPA
Sbjct: 406 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTVQQARTMNRHLTPTFEIPPA 465

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLSIFGILSMLTGLVLYERLLVPFA+RFTKNPSGITCLQRMGVGFAINILATLVSS +EI
Sbjct: 466 SLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGITCLQRMGVGFAINILATLVSSLIEI 525

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
           RRKKVAA H LLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA
Sbjct: 526 RRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 585

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVY 602
           T LYWLAIS+GNYVGTLMV LVHKYSGKEHNWLPDRNLNRGRLE YYWLVSGIQ LNLVY
Sbjct: 586 TALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDRNLNRGRLERYYWLVSGIQVLNLVY 603

Query: 603 YVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTNTA 648
           YVVCA FYT KPLEEE ++  E     SSS+ SKD  GCQ T TA
Sbjct: 646 YVVCAWFYTYKPLEEEKDDEGE----PSSSTLSKD--GCQGTKTA 603

BLAST of Spg008925 vs. ExPASy TrEMBL
Match: Q96400 (Nitrite transporter OS=Cucumis sativus OX=3659 GN=NiTR1 PE=2 SV=2)

HSP 1 Score: 946.8 bits (2446), Expect = 4.7e-272
Identity = 487/616 (79.06%), Postives = 510/616 (82.79%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNEVCDRFAS+GFH+NIITYLTQDLNM LVPASNILTNFAATSSFTSLIGALIADSFAGR
Sbjct: 47  GNEVCDRFASSGFHSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGR 106

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           F TI +ASIIYEL                                               
Sbjct: 107 FWTITIASIIYEL----------------------------------------------- 166

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GM TITISAILP LHPPPCPTQ+NC QASG
Sbjct: 167 ------------------------------GMVTITISAILPSLHPPPCPTQINCTQASG 226

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           TQL +LYL+LLLTSLG GGIRPCVV FAADQFDM+KVG+AGRTWNFFNWYYFCMG+ATLT
Sbjct: 227 TQLMILYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRTWNFFNWYYFCMGMATLT 286

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAA 
Sbjct: 287 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAF 346

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           + RKAVLP DSKLLYRNH+LDAAIA+QGRLVHTDQFKWLDKAAVITSPD T +     PN
Sbjct: 347 KNRKAVLPDDSKLLYRNHELDAAIAIQGRLVHTDQFKWLDKAAVITSPDSTAN----PPN 406

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
           LWRLATVHR+EELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA
Sbjct: 407 LWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 466

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           +LSIFGILSMLTGLVLY+RLLVPFAK+ T NPSGITCLQRMGVGFAINILATLVSS VEI
Sbjct: 467 TLSIFGILSMLTGLVLYDRLLVPFAKKLTHNPSGITCLQRMGVGFAINILATLVSSIVEI 526

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
           +RKKVAA HGLLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA
Sbjct: 527 KRKKVAANHGLLDNPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 581

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVY 602
           T LYWLAISVGNY+GTLMVYLVHKY+G+EHNWLPDRNLNRGRLEYYYWLVSGIQ +NLVY
Sbjct: 587 TALYWLAISVGNYIGTLMVYLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQVMNLVY 581

Query: 603 YVVCASFYTCKPLEEE 619
           YV+CA FYT KPLEEE
Sbjct: 647 YVICAWFYTYKPLEEE 581

BLAST of Spg008925 vs. ExPASy TrEMBL
Match: A0A1S4DUU1 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo OX=3656 GN=LOC103487163 PE=3 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 2.6e-270
Identity = 489/645 (75.81%), Postives = 519/645 (80.47%), Query Frame = 0

Query: 1   MTGNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFA 60
           + GNEVCDRFAS+GFH+NIITYLTQDLNM LVPASNILTNFAATSSFTSLIGALIADSFA
Sbjct: 12  IVGNEVCDRFASSGFHSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFA 71

Query: 61  GRFSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQG 120
           GRF TI +ASIIYEL                                             
Sbjct: 72  GRFWTITIASIIYEL--------------------------------------------- 131

Query: 121 LAQAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQA 180
                                           GM TITISAILP LHPPPCPTQ+NCIQA
Sbjct: 132 --------------------------------GMVTITISAILPSLHPPPCPTQINCIQA 191

Query: 181 SGTQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLAT 240
           SGTQL  LYL+LLLTSLG GGIRPCVV FAADQFDM+KVG+AGRTWNFFNWYYFCMG+AT
Sbjct: 192 SGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRTWNFFNWYYFCMGMAT 251

Query: 241 LTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVA 300
           LTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVA
Sbjct: 252 LTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVA 311

Query: 301 AVRKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEA 360
           A + RKAVLP DS+LLYRNH+LDAAIAVQG+LVHTDQFKWLDKAAVIT+PD T +     
Sbjct: 312 AFKNRKAVLPDDSELLYRNHELDAAIAVQGKLVHTDQFKWLDKAAVITTPDSTAN----P 371

Query: 361 PNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIP 420
           PNLWRLATVHR+EELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIP
Sbjct: 372 PNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIP 431

Query: 421 PASLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAV 480
           PASLSIFGILSMLTGLVLY+RLLVPFAK+ T NP GITCLQRMGVGFAINILATLVSSAV
Sbjct: 432 PASLSIFGILSMLTGLVLYDRLLVPFAKKLTHNPLGITCLQRMGVGFAINILATLVSSAV 491

Query: 481 EIRRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRS 540
           EI+RKKVAA HGLLD  TATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRS
Sbjct: 492 EIKRKKVAANHGLLDNPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRS 551

Query: 541 TATVLYWLAISVGNYVGTLMVYLVHKYSGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNL 600
           TAT LYWLAIS GNY+GTLMVYLVHKY+G+EHNWLPDRNLNRGRLEYYYWLVSGIQ +NL
Sbjct: 552 TATALYWLAISAGNYIGTLMVYLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQVINL 568

Query: 601 VYYVVCASFYTCKPLEEETEEGAEEKQLASSSSFSKDNNGCQQTN 646
           VYYV+CA FYT KPLE       EEK +  +    ++   C+ T+
Sbjct: 612 VYYVICAWFYTYKPLE-------EEKTIIENKDSDQEGERCEDTS 568

BLAST of Spg008925 vs. ExPASy TrEMBL
Match: A0A5A7UI29 (Protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006470 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 1.2e-254
Identity = 465/617 (75.36%), Postives = 492/617 (79.74%), Query Frame = 0

Query: 29  MALVPASNILTNFAATSSFTSLIGALIADSFAGRFSTIIVASIIYELVYIPLSLDTVKFH 88
           M LVPASNILTNFAATSSFTSLIGALIADSFAGRF TI +ASIIYEL             
Sbjct: 1   MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYEL------------- 60

Query: 89  FSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLAQAPHAAISITFPLGQLDAVSNSSKVI 148
                                                                       
Sbjct: 61  ------------------------------------------------------------ 120

Query: 149 LIFQGMATITISAILPKLHPPPCPTQLNCIQASGTQLTVLYLSLLLTSLGPGGIRPCVVT 208
               GM TITISAILP LHPPPCPTQ+NCIQASGTQL  LYL+LLLTSLG GGIRPCVV 
Sbjct: 121 ----GMVTITISAILPSLHPPPCPTQINCIQASGTQLMFLYLALLLTSLGAGGIRPCVVA 180

Query: 209 FAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMA 268
           FAADQFDM+KVG+AGRTWNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFGLPTIAMA
Sbjct: 181 FAADQFDMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMA 240

Query: 269 LSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAVRKRKAVLPHDSKLLYRNHKLDAAIAV 328
           LSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAA + RKAVLP DS+LLYRNH+LDAAIAV
Sbjct: 241 LSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPDDSELLYRNHELDAAIAV 300

Query: 329 QGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPNLWRLATVHRIEELKSIIRMLPIWAAG 388
           QG+LVHTDQFKWLDKAAVIT+PD T +     PNLWRLATVHR+EELKSIIRMLPIWAAG
Sbjct: 301 QGKLVHTDQFKWLDKAAVITTPDSTAN----PPNLWRLATVHRVEELKSIIRMLPIWAAG 360

Query: 389 ILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPASLSIFGILSMLTGLVLYERLLVPFAK 448
           ILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPASLSIFGILSMLTGLVLY+RLLVPFAK
Sbjct: 361 ILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPASLSIFGILSMLTGLVLYDRLLVPFAK 420

Query: 449 RFTKNPSGITCLQRMGVGFAINILATLVSSAVEIRRKKVAARHGLLDASTATIPFSVFWL 508
           + T NP GITCLQRMGVGFAINILATLVSSAVEI+RKKVAA HGLLD  TATIPFSVFWL
Sbjct: 421 KLTHNPLGITCLQRMGVGFAINILATLVSSAVEIKRKKVAANHGLLDNPTATIPFSVFWL 480

Query: 509 VPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATVLYWLAISVGNYVGTLMVYLVHKYS 568
           VPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTAT LYWLAIS GNY+GTLMVYLVHKY+
Sbjct: 481 VPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISAGNYIGTLMVYLVHKYT 529

Query: 569 GKEHNWLPDRNLNRGRLEYYYWLVSGIQALNLVYYVVCASFYTCKPLEEETEEGAEEKQL 628
           G+EHNWLPDRNLNRGRLEYYYWLVSGIQ +NLVYYV+CA FYT KPLE       EEK +
Sbjct: 541 GEEHNWLPDRNLNRGRLEYYYWLVSGIQVINLVYYVICAWFYTYKPLE-------EEKTI 529

Query: 629 ASSSSFSKDNNGCQQTN 646
             +    ++   C+ T+
Sbjct: 601 IENKDSDQEGERCEDTS 529

BLAST of Spg008925 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 619.0 bits (1595), Expect = 4.3e-177
Identity = 325/636 (51.10%), Postives = 417/636 (65.57%), Query Frame = 0

Query: 4   NEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGRF 63
           NE+C++ A  GFHAN+I+YLT  L++ L  A+N LTNFA TSS T L+GA IADSFAGRF
Sbjct: 36  NEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRF 95

Query: 64  STIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLAQ 123
            TI  ASIIY++                                                
Sbjct: 96  WTITFASIIYQI------------------------------------------------ 155

Query: 124 APHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASGT 183
                                        GM  +TISAI+P L PPPC  +  C+ A   
Sbjct: 156 -----------------------------GMTLLTISAIIPTLRPPPCKGEEVCVVADTA 215

Query: 184 QLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLTA 243
           QL++LY++LLL +LG GGIRPCVV F ADQFD S      +TWN+FNWYYFCMG A L A
Sbjct: 216 QLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLA 275

Query: 244 LTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAVR 303
           +TV+V+IQDNVGWG G G+PT+AM LSV+AFV G  LY  L P+GSP  RL QV VAA R
Sbjct: 276 VTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFR 335

Query: 304 KRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPNL 363
           KRK  +  D  LLY N ++DA I++ G+L HT    +LDKAA++T  +E N +  + PN 
Sbjct: 336 KRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIVT--EEDNLKPGQIPNH 395

Query: 364 WRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPAS 423
           WRL+TVHR+EELKS+IRM PI A+GILL+TA + Q +F++QQA+TMNRHLT +FQIP  S
Sbjct: 396 WRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGS 455

Query: 424 LSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEIR 483
           +S+F  ++MLT ++ Y+R+ V  A++FT    GIT L RMG+GF I+I+ATLV+  VE++
Sbjct: 456 MSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVK 515

Query: 484 RKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTAT 543
           RK VA  HGLLD     +P S  WL+PQ+ LHG+AE FMS+GHLEF YDQ+PES+RSTAT
Sbjct: 516 RKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTAT 575

Query: 544 VLYWLAISVGNYVGTLMVYLVHKYSGKE--HNWLPDRNLNRGRLEYYYWLVSGIQALNLV 603
            L+W+AIS+GNYV TL+V LVHK+S K    NWLPD NLNRGRLEY+YWL++ +QA+NLV
Sbjct: 576 ALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLV 592

Query: 604 YYVVCASFYTCKPLE-----EETEEGAEEKQLASSS 633
           YY+ CA  YT KP++     E++    EE QL++ S
Sbjct: 636 YYLWCAKIYTYKPVQVHHSKEDSSPVKEELQLSNRS 592

BLAST of Spg008925 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 397.5 bits (1020), Expect = 2.0e-110
Identity = 227/613 (37.03%), Postives = 334/613 (54.49%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           G E C+R A  G   N+I YL + +NM  V AS  ++N++ T   T LIGA IAD++ GR
Sbjct: 37  GTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGR 96

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           + TI                                                        
Sbjct: 97  YWTI-------------------------------------------------------- 156

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                +S V++   GM  +TISA +P L  P C  +     A+ 
Sbjct: 157 ---------------------ASFVVIYIAGMTLLTISASVPGL-TPTCSGE--TCHATA 216

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
            Q  + +++L L +LG GGI+PCV +F ADQFD +         +FFNW+YF + +  + 
Sbjct: 217 GQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGAMI 276

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           A +V+V+IQ NVGWGWG G+PT+AMA++VV F  GS  Y   KP GSPL R+ QV+VA+ 
Sbjct: 277 ASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVASC 336

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           RK K  +P D  LLY N   +++I    +L HT    + DKAAV T  D   +    +  
Sbjct: 337 RKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAAKSSS-- 396

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
            W+L TV ++EELK++IR+LPIWA GI+  +  S   +  + Q  T+++H+ P F+IP A
Sbjct: 397 -WKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSA 456

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLS+F  LS+L    +Y++L+VPFA+++T +  G T LQR+G+G  I+I + + +  +E+
Sbjct: 457 SLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEV 516

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
            R      H L +    TIP ++FW VPQ++L G AEVF  +G LEF YDQ+P+++RS  
Sbjct: 517 ARLNYVQTHNLYNEE--TIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLC 563

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKY--SGKEHNWLPDRNLNRGRLEYYYWLVSGIQALNL 602
           + L   AI+ GNY+ T +V LV K   SG    W+  +NLN G L+Y++WL++G+  LN 
Sbjct: 577 SALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIA-KNLNNGHLDYFFWLLAGLSFLNF 563

Query: 603 VYYVVCASFYTCK 614
           + Y+  A +YT K
Sbjct: 637 LVYLWIAKWYTYK 563

BLAST of Spg008925 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 386.7 bits (992), Expect = 3.6e-107
Identity = 224/613 (36.54%), Postives = 337/613 (54.98%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE C+R A  G   N+ITYLT  L+   V A+  +T +  T   T LIGA++AD++ GR
Sbjct: 53  GNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGR 112

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           + TI   S IY                                                 
Sbjct: 113 YWTIACFSGIY------------------------------------------------- 172

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                       F GM+ +T+SA +P L P  C     C  A+ 
Sbjct: 173 ----------------------------FIGMSALTLSASVPALKPAECIGDF-CPSATP 232

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
            Q  + +  L L +LG GGI+PCV +F ADQFD +      R  +FFNW+YF + +  L 
Sbjct: 233 AQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALV 292

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           + +++V+IQ+N GWG GFG+PT+ M L++ +F  G+PLY   KP GSP+ R++QVVVA+ 
Sbjct: 293 SSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASF 352

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           RK    +P D+ LLY     ++AIA   ++ HTD  ++LDKAAVI+   E  S++ +  N
Sbjct: 353 RKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS---EEESKSGDYSN 412

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
            WRL TV ++EELK +IRM PIWA+GI+     +   +  +QQ R MN  +  +FQ+PPA
Sbjct: 413 SWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKI-GSFQLPPA 472

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           +L  F   S++  + LY+R +VP A++FT    G T +QRMG+G  +++L    ++ VEI
Sbjct: 473 ALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEI 532

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
            R  +A   GL++ S A +P SV W +PQ+++ G AEVF  +G LEF YDQSP+++RS  
Sbjct: 533 IRLHMANDLGLVE-SGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLC 581

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGK--EHNWLPDRNLNRGRLEYYYWLVSGIQALNL 602
           + L  L  ++GNY+ +L++ LV  ++ +  +  W+ D NLN G L+Y++WL++G+  +N+
Sbjct: 593 SALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISD-NLNSGHLDYFFWLLAGLSLVNM 581

Query: 603 VYYVVCASFYTCK 614
             Y   A+ Y  K
Sbjct: 653 AVYFFSAARYKQK 581

BLAST of Spg008925 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 386.7 bits (992), Expect = 3.6e-107
Identity = 220/613 (35.89%), Postives = 326/613 (53.18%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE C+R A  G   N++ YL   LN     A+N +TN++ T   T LIGA IAD++ GR
Sbjct: 36  GNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGR 95

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           + TI     IY                                                 
Sbjct: 96  YWTIATFVFIY------------------------------------------------- 155

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCPTQLNCIQASG 182
                                         GM  +T+SA +P L P  C    +    + 
Sbjct: 156 ----------------------------VSGMTLLTLSASVPGLKPGNC--NADTCHPNS 215

Query: 183 TQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLATLT 242
           +Q  V +++L + +LG GGI+PCV +F ADQFD +      +  +FFNW+YF + +  L 
Sbjct: 216 SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALI 275

Query: 243 ALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAV 302
           A TV+V+IQ NVGWGWGFG+PT+AM ++V  F  GS  Y   +P GSPL R+ QV+VAA 
Sbjct: 276 AATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAF 335

Query: 303 RKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEAPN 362
           RK    +P D  LL+     ++ I    +LVHTD  K+ DKAAV     +++S  D   N
Sbjct: 336 RKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV---ESQSDSIKDGEVN 395

Query: 363 LWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPA 422
            WRL +V ++EELKSII +LP+WA GI+  T  S   +  + Q  TM++H+   F+IP A
Sbjct: 396 PWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSA 455

Query: 423 SLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAVEI 482
           SLS+F  +S+L    +Y++ ++P A++FT+N  G T LQRMG+G  ++I A + +  +E+
Sbjct: 456 SLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEV 515

Query: 483 RRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTA 542
            R      H   D     I  S+FW +PQ+ L G AEVF  +G LEF YDQ+P+++RS  
Sbjct: 516 VRLDYVKTHNAYDQK--QIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLC 563

Query: 543 TVLYWLAISVGNYVGTLMVYLVHKYSGK--EHNWLPDRNLNRGRLEYYYWLVSGIQALNL 602
           + L    +++GNY+ T++V +V K + K  +  W+PD NLNRG L+Y+++L++ +  LN 
Sbjct: 576 SALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYFFYLLATLSFLNF 563

Query: 603 VYYVVCASFYTCK 614
           + Y+  +  Y  K
Sbjct: 636 LVYLWISKRYKYK 563

BLAST of Spg008925 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 8.9e-106
Identity = 226/618 (36.57%), Postives = 339/618 (54.85%), Query Frame = 0

Query: 3   GNEVCDRFASTGFHANIITYLTQDLNMALVPASNILTNFAATSSFTSLIGALIADSFAGR 62
           GNE C+R A  G   N+ITY T +L+ + V A++ +  +  T   T LIGA+IADS+ GR
Sbjct: 64  GNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQGTCYITPLIGAVIADSYWGR 123

Query: 63  FSTIIVASIIYELVYIPLSLDTVKFHFSTLSKMIKRQKKMGEKTSAGAARTTKSSHQGLA 122
           + TI   S IY                                                 
Sbjct: 124 YWTIASFSAIY------------------------------------------------- 183

Query: 123 QAPHAAISITFPLGQLDAVSNSSKVILIFQGMATITISAILPKLHPPPCP--TQLNCIQA 182
                                       F GMA +T+SA LP L P  C       C  A
Sbjct: 184 ----------------------------FIGMALLTLSASLPVLKPAACAGVAAALCSPA 243

Query: 183 SGTQLTVLYLSLLLTSLGPGGIRPCVVTFAADQFDMSKVGVAGRTWNFFNWYYFCMGLAT 242
           +  Q  V +  L L +LG GGI+PCV +F ADQFD +      R  +FFNW+YF + + +
Sbjct: 244 TTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINIGS 303

Query: 243 LTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVA 302
             + T++V++Q+NVGWG GF +PT+ M +S+ +F +G+PLY   KP GSP+ R+ QV+VA
Sbjct: 304 FISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVLVA 363

Query: 303 AVRKRKAVLPHDSKLLYRNHKLDAAIAVQGRLVHTDQFKWLDKAAVITSPDETNSRNDEA 362
           A RK K  LP D   LY   + ++ IA   ++ HTD +K+LDKAAVI+   E  S++   
Sbjct: 364 AYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVIS---EYESKSGAF 423

Query: 363 PNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIP 422
            N W+L TV ++EE+K++IRM PIWA+GI+     S   +  +QQ R+MNR +  +F+IP
Sbjct: 424 SNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNR-IIRSFEIP 483

Query: 423 PASLSIFGILSMLTGLVLYERLLVPFAKRFTKNPSGITCLQRMGVGFAINILATLVSSAV 482
           PAS  +F  L +L  + +Y+R LVPF +RFT  P G+T LQRMG+G  +++L+   ++ V
Sbjct: 484 PASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIV 543

Query: 483 EIRRKKVAARHGLLDASTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRS 542
           E  R ++A            +  S+FW +PQ+ L GIAEVF  +G +EF YD+SP+++RS
Sbjct: 544 ETVRLQLAQDF---------VAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRS 590

Query: 543 TATVLYWLAISVGNYVGTLMVYLVHKYS--GKEHNWLPDRNLNRGRLEYYYWLVSGIQAL 602
             + L  L  +VG+Y+ +L++ LV  ++  G +  W+PD +LN+G L+Y++WL+  +  +
Sbjct: 604 VCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPD-DLNKGHLDYFFWLLVSLGLV 590

Query: 603 NL-VYYVVCASFYTCKPL 616
           N+ VY ++C      K L
Sbjct: 664 NIPVYALICVKHTKKKAL 590

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6596175.11.1e-27578.45Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7027713.11.1e-27578.45Protein NRT1/ PTR FAMILY 3.1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022971207.13.2e-27578.29protein NRT1/ PTR FAMILY 3.1-like [Cucurbita maxima][more]
XP_023540126.14.2e-27578.45protein NRT1/ PTR FAMILY 3.1-like [Cucurbita pepo subsp. pepo][more]
XP_022941237.11.6e-27478.29protein NRT1/ PTR FAMILY 3.1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SX206.1e-17651.10Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q9LFB82.9e-10937.03Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Q9M3905.1e-10635.89Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
P460325.1e-10636.54Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z201.2e-10436.57Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I1C51.6e-27578.29protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita maxima OX=3661 GN=LOC111469999 PE... [more]
A0A6J1FLW37.7e-27578.29protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita moschata OX=3662 GN=LOC111446603 ... [more]
Q964004.7e-27279.06Nitrite transporter OS=Cucumis sativus OX=3659 GN=NiTR1 PE=2 SV=2[more]
A0A1S4DUU12.6e-27075.81protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo OX=3656 GN=LOC103487163 PE=3 S... [more]
A0A5A7UI291.2e-25475.36Protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G68570.14.3e-17751.10Major facilitator superfamily protein [more]
AT5G01180.12.0e-11037.03peptide transporter 5 [more]
AT2G02040.13.6e-10736.54peptide transporter 2 [more]
AT3G54140.13.6e-10735.89peptide transporter 1 [more]
AT1G62200.18.9e-10636.57Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 148..575
e-value: 3.6E-86
score: 289.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 2..90
coord: 151..624
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..122
e-value: 1.3E-22
score: 81.7
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 138..618
e-value: 6.5E-144
score: 482.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 180..620
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 621..647
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 626..647
NoneNo IPR availablePANTHERPTHR11654:SF326NITRATE TRANSPORTER 1coord: 151..624
NoneNo IPR availablePANTHERPTHR11654:SF326NITRATE TRANSPORTER 1coord: 2..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008925.1Spg008925.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity