Homology
BLAST of Spg008805 vs. NCBI nr
Match:
XP_022923630.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata])
HSP 1 Score: 1000.7 bits (2586), Expect = 5.0e-288
Identity = 491/576 (85.24%), Postives = 524/576 (90.97%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ LSKCDANYA LTPITFLKRASA Y+DRTSIIYEGTRFTW QTY+RCCRLASSL RLN
Sbjct: 1 MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
V KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+ FVDY+
Sbjct: 61 VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
+IQ+AK+AL L A S LP VVVIDDIDSPTGAR G LEYEQL+H GDVGFTPVEVDD
Sbjct: 121 FIQEAKDALRQLMAASL-PLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTNVCMRNTTAFDI+RNINLHHV+HMCCAPIVFRIIL+A+ G +R G LVN
Sbjct: 241 TWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPA LLEKMEALGFHITHAYGLTEATGP LVCEWQEKWN+LPA EQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAM HPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
PCAFV VH G VSEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAK 540
Query: 541 LFNVSKKEVAGGNNSDQTDDGWQLGYGEHVLTRSRL 573
L S+K V+GGN S + D G Q YG+HV+ SRL
Sbjct: 541 LLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575
BLAST of Spg008805 vs. NCBI nr
Match:
XP_023513711.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1000.0 bits (2584), Expect = 8.5e-288
Identity = 491/576 (85.24%), Postives = 524/576 (90.97%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ LSKCDANYA LTPITFLKRASA Y+DRTSIIYEGTRFTW QTYERCCRLASSL RLN
Sbjct: 1 MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLSRLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
V KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+ FVDY+
Sbjct: 61 VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
+IQ+AK+AL L A S LP VVVIDDIDSPTGAR G LEYEQL+H GDVGFTPVEVDD
Sbjct: 121 FIQEAKDALRQLMAASL-PLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLV+GWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVVGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTNVCMRNTTAFDI+RNINLHHV+HMCCAPIVFRIIL+A+ G +R G LVN
Sbjct: 241 TWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPA LLEKMEALGFHITHAYGLTEATGP LVCEWQEKWN+LPA EQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAM HPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
PCAFV VH G VSEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAK 540
Query: 541 LFNVSKKEVAGGNNSDQTDDGWQLGYGEHVLTRSRL 573
L S+K V+GGN S + D G Q YG+HV+ SRL
Sbjct: 541 LLVGSEKRVSGGNKSGRHDGGRQPEYGQHVMAMSRL 575
BLAST of Spg008805 vs. NCBI nr
Match:
KAG6602004.1 (Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 996.9 bits (2576), Expect = 7.2e-287
Identity = 490/576 (85.07%), Postives = 523/576 (90.80%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ LSKCDANYA LTPITFLKRASA Y+DRTSIIYEGTRFTW QTY+RCCRLASSL RLN
Sbjct: 1 MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
V KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+ FVDY+
Sbjct: 61 VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
+IQ+AK+AL L A S LP VVVIDDIDSPTGAR G LEYEQL+H GDVGFTPVEVDD
Sbjct: 121 FIQEAKDALRQLMAASL-PLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTNVCMRNTTAFDI+RNINLHHV+HMCCAPIVFRIIL+A+ G +R G LVN
Sbjct: 241 TWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPA LLEKMEALGFHITHAYGLTEATGP LVCEWQEKWN+LPA EQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKD AATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDLAATAKAFQNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAM HPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
PCAFV VH G VSEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAK 540
Query: 541 LFNVSKKEVAGGNNSDQTDDGWQLGYGEHVLTRSRL 573
L S+K V+GGN S + D G Q YG+HV+ SRL
Sbjct: 541 LLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575
BLAST of Spg008805 vs. NCBI nr
Match:
XP_022990938.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima])
HSP 1 Score: 992.6 bits (2565), Expect = 1.4e-285
Identity = 487/576 (84.55%), Postives = 521/576 (90.45%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ LSKCDAN+A LTPITFLKRASA Y+DRTSIIYEGT FTW QTYERCC LASSL RLN
Sbjct: 1 MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
V KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+ FVDY+
Sbjct: 61 VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
+IQ+AK+AL L A S LP VVVIDDIDSPTGAR G LEYEQL+H GDVGFTPVEVDD
Sbjct: 121 FIQEAKDALRQLMAASL-PLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTNVC+RNTTAFDI+RNINLHHV+HMCCAPIVFRIIL+A+ G +R G LVN
Sbjct: 241 TWGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPA LLEKMEALGFHITHAYGLTEATGP LVCEWQEKWN+LPA EQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAM HPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
PCAFV VH G VSEAE+I FCRKN+SHYM PKKV+FVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAK 540
Query: 541 LFNVSKKEVAGGNNSDQTDDGWQLGYGEHVLTRSRL 573
L S+K V+GGN S + D G YGEHV+ SRL
Sbjct: 541 LLVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSRL 575
BLAST of Spg008805 vs. NCBI nr
Match:
XP_038906606.1 (trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida])
HSP 1 Score: 969.9 bits (2506), Expect = 9.4e-279
Identity = 477/578 (82.53%), Postives = 513/578 (88.75%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
MD L KCDANY LTPITFL RAS Y+DRTSIIYEGTRFTWHQTYERCCRLASSL LN
Sbjct: 1 MDMLLKCDANYTSLTPITFLTRASTFYADRTSIIYEGTRFTWHQTYERCCRLASSLRHLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
++KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+ FVDY+
Sbjct: 61 ISKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIAVILRHSEAKILFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
YIQ+AK+AL +L A+S +PLVV+IDDIDSPTG R G+ EYE+LIH GDV FTP+EVDD
Sbjct: 121 YIQEAKDALRLLMAKS-FPMPLVVIIDDIDSPTGIRFGNFEYEKLIHDGDVNFTPLEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTN+CMRNTTA DIFRNINLHHV+HMCCAPIVFRIILDA++ R+ +N
Sbjct: 241 TWGIAARGGTNICMRNTTAIDIFRNINLHHVTHMCCAPIVFRIILDANSHDRCRVAWPIN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPAPLLEKMEALGFHITHAYGLTEATGP LVCEWQEKWN LPA +QAKLKARQ
Sbjct: 301 VLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNTLPADKQAKLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNLDTMESVPHDG T+GEIVLRGS +MKGYFKDP ATAAAF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLDTMESVPHDGHTTGEIVLRGSGLMKGYFKDPVATAAAFKNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GD+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLY HPQVAEAAVVAM HPRWGES
Sbjct: 421 GDIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYSHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVHGG---VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
PCAFVAVHG VSEAEIIAFCRKNL HYMVPKKVVFV ELPK + GKIQKFKLREQAK
Sbjct: 481 PCAFVAVHGDAGRVSEAEIIAFCRKNLPHYMVPKKVVFVGELPK-ANGKIQKFKLREQAK 540
Query: 541 LFNVSKKEV--AGGNNSDQTDDGWQLGYGEHVLTRSRL 573
VS+K V G N S + D + YGEHVL SRL
Sbjct: 541 YLEVSEKRVVGTGWNKSSRQDGNRRSDYGEHVLAMSRL 576
BLAST of Spg008805 vs. ExPASy Swiss-Prot
Match:
I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)
HSP 1 Score: 742.7 bits (1916), Expect = 3.2e-213
Identity = 362/553 (65.46%), Postives = 429/553 (77.58%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
MD+L KC ANY PLTP+TFL RA SY++RTSIIY G RFTW QTY+RCCRLASSL LN
Sbjct: 1 MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
+ KN VSV+APN+PA YEMHFAVPMAGAVLNTINTRLD NIAIIL+HSEAK+ FVDYE
Sbjct: 61 IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120
Query: 121 YIQQAKEALHVLAAESAST--------LPLVVVIDDIDSPTG-ARLGSLEYEQLIHRGDV 180
Y+++A++AL +L + + T +P V++IDD+ SPT + LEYEQL+H+G+
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTRIQQQDQLEYEQLVHQGNP 180
Query: 181 GFTPVE-VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTL 240
+ P VDDEWD I LNYTSGTTS PKGVVYSHRGAFLSTL+ +MGWEMG PVYLW+L
Sbjct: 181 EYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPVYLWSL 240
Query: 241 PMFHCNGWTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNG 300
PMFH NGWT TWGIAARGGTNVC+RNTTA +I+ NI LH V+HMCCAP VF I+L+A
Sbjct: 241 PMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILLEAKPH 300
Query: 301 SPKRIGSLVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPA 360
+ I + V VM GGAPPP L+ K+E LGFH+ H YG+TEA G LVCEWQ +WN L
Sbjct: 301 ERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEWNKLSR 360
Query: 361 AEQAKLKARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATA 420
+QA LKARQGIS+L L DVDVKN TM+SVPH+G+T GEI LRGSSIMKGYFK+ A +
Sbjct: 361 EDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKNDKANS 420
Query: 421 AAFRNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVV 480
F+NGWFLTGDV VIH DGYLEIKDR KD+IISGGENISS+E+E + +HP V EAAVV
Sbjct: 421 QVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVIEAAVV 480
Query: 481 AMSHPRWGESPCAFV--AVHGGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQ 540
AM HPRWGE+PCAFV + + EA+II C+K L +MVPK V F+ ELPKT TGK++
Sbjct: 481 AMPHPRWGETPCAFVIKTKNPEIKEADIIVHCKKELPGFMVPKHVQFLEELPKTGTGKVK 540
BLAST of Spg008805 vs. ExPASy Swiss-Prot
Match:
Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)
HSP 1 Score: 687.2 bits (1772), Expect = 1.6e-196
Identity = 336/563 (59.68%), Postives = 419/563 (74.42%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
MD L C+AN PLTPITFLKRAS Y +RTSIIY TRFTW QTY+RCCRLA+SL LN
Sbjct: 1 MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
+T+N VS++APN+PA+YEMHF+VPM GAVLN INTRLD K IAIILRH+E K+ FVDYE
Sbjct: 61 ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGD----VGFTPV 180
+ +E L ++ + P +++I++IDS T L+YE LI +G+ +
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180
Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
V +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240
Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIG 300
GWT TW +AARGGTNVC+R+ TA +I++NI LH V+HM C P VFR +L+
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300
Query: 301 SLVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKL 360
S V V+TGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ++WN LP +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360
Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
+ RQG+ LTLADVDVKN T+ESVP DG+T GEIV++GSS+MKGY K+P AT+ AF++G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420
Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPR 480
W TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAM HP
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480
Query: 481 WGESPCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFK 540
WGE+PCAFV + G SE ++I +CR+N+ H+M PKKVVF ELPK S GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540
Query: 541 LREQAKLFNVSKKEVAGGNNSDQ 554
LR+ AK V +++ AG Q
Sbjct: 541 LRDIAKAL-VVREDDAGSKKVHQ 562
BLAST of Spg008805 vs. ExPASy Swiss-Prot
Match:
Q9SS01 (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 SV=1)
HSP 1 Score: 681.0 bits (1756), Expect = 1.1e-194
Identity = 340/569 (59.75%), Postives = 421/569 (73.99%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
MD L+ C+AN PLTP+TFLKRAS Y +RTSIIY TRFTW QTY+RCCRLA+SL LN
Sbjct: 1 MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
++KN VSV+APN PALYEMHFAVPMAGAVLN INTRLD +IA ILRH++ K+ F+D
Sbjct: 61 ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPV---- 180
+ A+E+LH+L++E ++ V+ I + D P A L+YE LI RG+ + V
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180
Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
+ DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240
Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIG 300
GWTFTWG AARGGT+VCMR+ TA +I++NI +H+V+HMCC P VF I+L ++
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300
Query: 301 SLVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKL 360
V+V+TGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ++WN LP +Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360
Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
KARQGISIL LADVDVKN +T +S P DG+T GEI+++GSSIMKGY K+P AT AF++G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420
Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPR 480
W TGDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAM HP
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480
Query: 481 WGESPCAFVAVHGG-------------VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTS 540
WGE+PCAFV + E +I +CR+NL H+M P+KVVF+ ELPK
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540
Query: 541 TGKIQKFKLREQAK------LFNVSKKEV 546
GKI K KLR+ AK NV KEV
Sbjct: 541 NGKILKPKLRDIAKGLVVEDEINVIAKEV 569
BLAST of Spg008805 vs. ExPASy Swiss-Prot
Match:
Q9SS00 (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE12 PE=2 SV=1)
HSP 1 Score: 677.2 bits (1746), Expect = 1.7e-193
Identity = 332/555 (59.82%), Postives = 416/555 (74.95%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
MD L+ C+AN PLTPITFLKRAS Y +RTSIIY TRFTW QTY+RCCRLA+SL LN
Sbjct: 1 MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
+ KN VSV+APN PA+YEMHFAVPMAGAVLN INTRLD +IA ILRH++ K+ F+
Sbjct: 61 IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVE--- 180
+ A+E L +L++E ++ V+ I +ID P +YE LI RG+ TP+
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEP--TPLLLAR 180
Query: 181 ---VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFH 240
+ DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG PVYLWTLPMFH
Sbjct: 181 MFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFH 240
Query: 241 CNGWTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKR 300
CNGWTFTWG AARGGT+VCMR+ TA +I++NI +H+V+HMCC P VF I+L ++
Sbjct: 241 CNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSH 300
Query: 301 IGSLVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQA 360
V+V+TGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ++WN LP +Q
Sbjct: 301 RSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQM 360
Query: 361 KLKARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFR 420
+LKARQG+SIL L +VDV+N +T ESVP DG+T GEIV++GSSIMKGY K+P AT AF+
Sbjct: 361 ELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFK 420
Query: 421 NGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSH 480
+GW +GDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAM H
Sbjct: 421 HGWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPH 480
Query: 481 PRWGESPCAFVAVHGG------------VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKT 537
P WGE+PCAFV + G E ++I +CR+NL H+M P+KVVF+ ELPK
Sbjct: 481 PTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKN 540
BLAST of Spg008805 vs. ExPASy Swiss-Prot
Match:
F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)
HSP 1 Score: 575.1 bits (1481), Expect = 8.9e-163
Identity = 281/550 (51.09%), Postives = 387/550 (70.36%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ K ANY PLTPI+FL R++ Y+DR SI+Y ++TW QT +RC R+AS+L +L
Sbjct: 3 MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62
Query: 61 V-TKNGVSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
+ T + VSV+APN+PA+ E+HF VPMAGA+L T+N R D +A++LRHS KV F D++
Sbjct: 63 ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGS---LEYEQLIHRGDVGFTPVE 180
++Q A+ A +L+ + LV++ + + + S +EYE ++ G F +
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182
Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+ EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242
Query: 241 WTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGS 300
W WG+ A GGTN+C+RN TA IF NI+ H V+HM AP + +I++A K +
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302
Query: 301 LVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLK 360
V+ +TG APPPA ++ KME LGF + H+YGLTE GPG +C W+ +W+ LP EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362
Query: 361 ARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGW 420
ARQG++ L L ++ VK+ TM ++P DG T GE+V RG+++M GY K+P AT AF+ GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422
Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRW 480
F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482
Query: 481 GESPCAFVAVHGG--VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
GE+ CAFV + G S E+I++CR L HYM P+ +VF +LPKTSTGK+QKF LR +
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRTK 542
Query: 541 AK-LFNVSKK 544
AK L ++SKK
Sbjct: 543 AKALVSLSKK 551
BLAST of Spg008805 vs. ExPASy TrEMBL
Match:
A0A6J1ECD9 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431279 PE=4 SV=1)
HSP 1 Score: 1000.7 bits (2586), Expect = 2.4e-288
Identity = 491/576 (85.24%), Postives = 524/576 (90.97%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ LSKCDANYA LTPITFLKRASA Y+DRTSIIYEGTRFTW QTY+RCCRLASSL RLN
Sbjct: 1 MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
V KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+ FVDY+
Sbjct: 61 VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
+IQ+AK+AL L A S LP VVVIDDIDSPTGAR G LEYEQL+H GDVGFTPVEVDD
Sbjct: 121 FIQEAKDALRQLMAASL-PLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTNVCMRNTTAFDI+RNINLHHV+HMCCAPIVFRIIL+A+ G +R G LVN
Sbjct: 241 TWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPA LLEKMEALGFHITHAYGLTEATGP LVCEWQEKWN+LPA EQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAM HPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
PCAFV VH G VSEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAK 540
Query: 541 LFNVSKKEVAGGNNSDQTDDGWQLGYGEHVLTRSRL 573
L S+K V+GGN S + D G Q YG+HV+ SRL
Sbjct: 541 LLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575
BLAST of Spg008805 vs. ExPASy TrEMBL
Match:
A0A6J1JUR4 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111487678 PE=4 SV=1)
HSP 1 Score: 992.6 bits (2565), Expect = 6.6e-286
Identity = 487/576 (84.55%), Postives = 521/576 (90.45%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ LSKCDAN+A LTPITFLKRASA Y+DRTSIIYEGT FTW QTYERCC LASSL RLN
Sbjct: 1 MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
V KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+ FVDY+
Sbjct: 61 VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
+IQ+AK+AL L A S LP VVVIDDIDSPTGAR G LEYEQL+H GDVGFTPVEVDD
Sbjct: 121 FIQEAKDALRQLMAASL-PLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTNVC+RNTTAFDI+RNINLHHV+HMCCAPIVFRIIL+A+ G +R G LVN
Sbjct: 241 TWGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPA LLEKMEALGFHITHAYGLTEATGP LVCEWQEKWN+LPA EQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAM HPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
PCAFV VH G VSEAE+I FCRKN+SHYM PKKV+FVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAK 540
Query: 541 LFNVSKKEVAGGNNSDQTDDGWQLGYGEHVLTRSRL 573
L S+K V+GGN S + D G YGEHV+ SRL
Sbjct: 541 LLVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSRL 575
BLAST of Spg008805 vs. ExPASy TrEMBL
Match:
A0A5A7TXW0 (Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold284G00160 PE=4 SV=1)
HSP 1 Score: 968.8 bits (2503), Expect = 1.0e-278
Identity = 480/579 (82.90%), Postives = 520/579 (89.81%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ L KCDANY LTPITFLKRASA Y+DRTSIIYEGTRFTW+QTYERCCRLASSL RL+
Sbjct: 1 MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
V+KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDVKNIA+ILRHSEAK+FFVDY+
Sbjct: 61 VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
YIQ+AK+AL +L AES T+PLVVVIDDID+PTGARLG+LEYEQLIH GD FTPVEVDD
Sbjct: 121 YIQEAKDALRLLVAES-FTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTN+CMRNTTA DIFRNINLH V+HMCCAPIVFRIIL+ADNG +R+ VN
Sbjct: 241 TWGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPA LLEKMEALGFHITHAYGLTEATGP LVCEWQEKWNVLPA +QA LKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNL TMESVPHDGRT+GEIVLRGSS+MKGYFKDP ATAAAF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GD+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAM HPRWGES
Sbjct: 421 GDIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
PCAFV +HGG VSEAE+IAFCRKNLSHYMVPKKVVF+AELPK + GK+QKF LREQ
Sbjct: 481 PCAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLREQ 540
Query: 541 AKLFNVSKKEVAGGNNSDQTDDGWQLGYG-EHVLTRSRL 573
AK VS+K +N + YG +HV+ SRL
Sbjct: 541 AKYLEVSEKRRV--SNLGNKSGNRRSDYGDQHVIAMSRL 575
BLAST of Spg008805 vs. ExPASy TrEMBL
Match:
A0A1S3CIM1 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103501376 PE=4 SV=1)
HSP 1 Score: 968.8 bits (2503), Expect = 1.0e-278
Identity = 480/579 (82.90%), Postives = 520/579 (89.81%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ L KCDANY LTPITFLKRASA Y+DRTSIIYEGTRFTW+QTYERCCRLASSL RL+
Sbjct: 1 MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
V+KN VSV+APN+PALYEMHFAVPMAGA+LNTINTRLDVKNIA+ILRHSEAK+FFVDY+
Sbjct: 61 VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDD 180
YIQ+AK+AL +L AES T+PLVVVIDDID+PTGARLG+LEYEQLIH GD FTPVEVDD
Sbjct: 121 YIQEAKDALRLLVAES-FTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDD 180
Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVN 300
TWGIAARGGTN+CMRNTTA DIFRNINLH V+HMCCAPIVFRIIL+ADNG +R+ VN
Sbjct: 241 TWGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVN 300
Query: 301 VMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQ 360
V+TGGAPPPA LLEKMEALGFHITHAYGLTEATGP LVCEWQEKWNVLPA +QA LKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQ 360
Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
GISILTLADVDVKNL TMESVPHDGRT+GEIVLRGSS+MKGYFKDP ATAAAF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLT 420
Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGES 480
GD+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAM HPRWGES
Sbjct: 421 GDIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480
Query: 481 PCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
PCAFV +HGG VSEAE+IAFCRKNLSHYMVPKKVVF+AELPK + GK+QKF LREQ
Sbjct: 481 PCAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLREQ 540
Query: 541 AKLFNVSKKEVAGGNNSDQTDDGWQLGYG-EHVLTRSRL 573
AK VS+K +N + YG +HV+ SRL
Sbjct: 541 AKYLEVSEKRRV--SNLGNKSGNRRSDYGDQHVIAMSRL 575
BLAST of Spg008805 vs. ExPASy TrEMBL
Match:
A0A0A0KT40 (Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G319910 PE=4 SV=1)
HSP 1 Score: 960.3 bits (2481), Expect = 3.6e-276
Identity = 473/575 (82.26%), Postives = 515/575 (89.57%), Query Frame = 0
Query: 4 LSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLNVTK 63
L KCDANY+ LTPITFLKRASA Y+DRTSIIYEGTRFTW QTYERCCRLASSL RL V+K
Sbjct: 2 LLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLTVSK 61
Query: 64 NG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYEYIQ 123
N VSV+APN+PALYEMHFAVPMAGA+LNTINTRLD+KNIA+ILRHSEAK+FFVDY+YIQ
Sbjct: 62 NDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQYIQ 121
Query: 124 QAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVEVDDEWD 183
+AK+AL +L AES S +PLVVVIDDID+PTGARLG+LEYEQLI+ GD FTPVEVDDEWD
Sbjct: 122 EAKDALRLLVAES-SPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDEWD 181
Query: 184 SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG 243
SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG
Sbjct: 182 SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG 241
Query: 244 IAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGSLVNVMT 303
IAARGGTN+CMRNTTA DIFRNINLH V+HMCCAPIVF IIL+ADN +R+ VNV+T
Sbjct: 242 IAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNVLT 301
Query: 304 GGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLKARQGIS 363
GGAPPPAPLLEKMEALGFHITHAYGLTEATGP LVCEWQEKWNVLPA +QA LKARQGIS
Sbjct: 302 GGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQGIS 361
Query: 364 ILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLTGDV 423
ILTLADVDVKNL TMESVPHDGRT+GEIVLRGSS+MKGYFKDPAATAAA +NGWFLTGD+
Sbjct: 362 ILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTGDI 421
Query: 424 GVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRWGESPCA 483
GVI+PDGY+EIKDRSKDVIISGGENISSVE+ETVLYRHPQV+EAAVVAM HPRWGESPCA
Sbjct: 422 GVIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESPCA 481
Query: 484 FVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 543
FV + G VSEAE++AFCRKNLSHYMVPKKVVFVAELPK + GK+QKF LREQAK
Sbjct: 482 FVVLQDGGGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLREQAKY 541
Query: 544 FNVSKKEVAGGNNSDQTDDGWQLGYGEHVLTRSRL 573
VS+K + +N + YGEHV+ SRL
Sbjct: 542 LEVSEKRIV--SNMGNKSGNRRSDYGEHVIAMSRL 572
BLAST of Spg008805 vs. TAIR 10
Match:
AT1G66120.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 687.2 bits (1772), Expect = 1.1e-197
Identity = 336/563 (59.68%), Postives = 419/563 (74.42%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
MD L C+AN PLTPITFLKRAS Y +RTSIIY TRFTW QTY+RCCRLA+SL LN
Sbjct: 1 MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
+T+N VS++APN+PA+YEMHF+VPM GAVLN INTRLD K IAIILRH+E K+ FVDYE
Sbjct: 61 ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGD----VGFTPV 180
+ +E L ++ + P +++I++IDS T L+YE LI +G+ +
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180
Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
V +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240
Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIG 300
GWT TW +AARGGTNVC+R+ TA +I++NI LH V+HM C P VFR +L+
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300
Query: 301 SLVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKL 360
S V V+TGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ++WN LP +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360
Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
+ RQG+ LTLADVDVKN T+ESVP DG+T GEIV++GSS+MKGY K+P AT+ AF++G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420
Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPR 480
W TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAM HP
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480
Query: 481 WGESPCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFK 540
WGE+PCAFV + G SE ++I +CR+N+ H+M PKKVVF ELPK S GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540
Query: 541 LREQAKLFNVSKKEVAGGNNSDQ 554
LR+ AK V +++ AG Q
Sbjct: 541 LRDIAKAL-VVREDDAGSKKVHQ 562
BLAST of Spg008805 vs. TAIR 10
Match:
AT1G65880.1 (benzoyloxyglucosinolate 1 )
HSP 1 Score: 681.0 bits (1756), Expect = 8.2e-196
Identity = 340/569 (59.75%), Postives = 421/569 (73.99%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
MD L+ C+AN PLTP+TFLKRAS Y +RTSIIY TRFTW QTY+RCCRLA+SL LN
Sbjct: 1 MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
++KN VSV+APN PALYEMHFAVPMAGAVLN INTRLD +IA ILRH++ K+ F+D
Sbjct: 61 ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPV---- 180
+ A+E+LH+L++E ++ V+ I + D P A L+YE LI RG+ + V
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180
Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
+ DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240
Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIG 300
GWTFTWG AARGGT+VCMR+ TA +I++NI +H+V+HMCC P VF I+L ++
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300
Query: 301 SLVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKL 360
V+V+TGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ++WN LP +Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360
Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
KARQGISIL LADVDVKN +T +S P DG+T GEI+++GSSIMKGY K+P AT AF++G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420
Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPR 480
W TGDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAM HP
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480
Query: 481 WGESPCAFVAVHGG-------------VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTS 540
WGE+PCAFV + E +I +CR+NL H+M P+KVVF+ ELPK
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540
Query: 541 TGKIQKFKLREQAK------LFNVSKKEV 546
GKI K KLR+ AK NV KEV
Sbjct: 541 NGKILKPKLRDIAKGLVVEDEINVIAKEV 569
BLAST of Spg008805 vs. TAIR 10
Match:
AT1G65890.1 (acyl activating enzyme 12 )
HSP 1 Score: 677.2 bits (1746), Expect = 1.2e-194
Identity = 332/555 (59.82%), Postives = 416/555 (74.95%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
MD L+ C+AN PLTPITFLKRAS Y +RTSIIY TRFTW QTY+RCCRLA+SL LN
Sbjct: 1 MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60
Query: 61 VTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
+ KN VSV+APN PA+YEMHFAVPMAGAVLN INTRLD +IA ILRH++ K+ F+
Sbjct: 61 IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFTPVE--- 180
+ A+E L +L++E ++ V+ I +ID P +YE LI RG+ TP+
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEP--TPLLLAR 180
Query: 181 ---VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFH 240
+ DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG PVYLWTLPMFH
Sbjct: 181 MFCIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFH 240
Query: 241 CNGWTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKR 300
CNGWTFTWG AARGGT+VCMR+ TA +I++NI +H+V+HMCC P VF I+L ++
Sbjct: 241 CNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSH 300
Query: 301 IGSLVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQA 360
V+V+TGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ++WN LP +Q
Sbjct: 301 RSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQM 360
Query: 361 KLKARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFR 420
+LKARQG+SIL L +VDV+N +T ESVP DG+T GEIV++GSSIMKGY K+P AT AF+
Sbjct: 361 ELKARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFK 420
Query: 421 NGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSH 480
+GW +GDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAM H
Sbjct: 421 HGWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPH 480
Query: 481 PRWGESPCAFVAVHGG------------VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKT 537
P WGE+PCAFV + G E ++I +CR+NL H+M P+KVVF+ ELPK
Sbjct: 481 PTWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKN 540
BLAST of Spg008805 vs. TAIR 10
Match:
AT1G20560.1 (acyl activating enzyme 1 )
HSP 1 Score: 575.1 bits (1481), Expect = 6.3e-164
Identity = 281/550 (51.09%), Postives = 387/550 (70.36%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWHQTYERCCRLASSLHRLN 60
M+ K ANY PLTPI+FL R++ Y+DR SI+Y ++TW QT +RC R+AS+L +L
Sbjct: 3 MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62
Query: 61 V-TKNGVSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDYE 120
+ T + VSV+APN+PA+ E+HF VPMAGA+L T+N R D +A++LRHS KV F D++
Sbjct: 63 ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122
Query: 121 YIQQAKEALHVLAAESASTLPLVVVIDDIDSPTGARLGS---LEYEQLIHRGDVGFTPVE 180
++Q A+ A +L+ + LV++ + + + S +EYE ++ G F +
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182
Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+ EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242
Query: 241 WTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPKRIGS 300
W WG+ A GGTN+C+RN TA IF NI+ H V+HM AP + +I++A K +
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302
Query: 301 LVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQAKLK 360
V+ +TG APPPA ++ KME LGF + H+YGLTE GPG +C W+ +W+ LP EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362
Query: 361 ARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGW 420
ARQG++ L L ++ VK+ TM ++P DG T GE+V RG+++M GY K+P AT AF+ GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422
Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMSHPRW 480
F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482
Query: 481 GESPCAFVAVHGG--VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
GE+ CAFV + G S E+I++CR L HYM P+ +VF +LPKTSTGK+QKF LR +
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRTK 542
Query: 541 AK-LFNVSKK 544
AK L ++SKK
Sbjct: 543 AKALVSLSKK 551
BLAST of Spg008805 vs. TAIR 10
Match:
AT5G16370.1 (acyl activating enzyme 5 )
HSP 1 Score: 565.1 bits (1455), Expect = 6.5e-161
Identity = 282/554 (50.90%), Postives = 379/554 (68.41%), Query Frame = 0
Query: 1 MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIY-EGTRFTWHQTYERCCRLASSLHRL 60
M+++ C AN PLTPI FL+RA+ Y D TSI+Y T +TW +T RC R+ASSL +
Sbjct: 1 MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60
Query: 61 NVTKNG-VSVIAPNIPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHSEAKVFFVDY 120
+ ++ VSV++PN PA+YE+ FAVPM+GA+LN INTRLD + ++++LRH +K+ FVD
Sbjct: 61 GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120
Query: 121 EYIQQAKEALHVLAAESASTLPLVVVIDDIDSPTG------ARLGSLEYEQLIHRGDVGF 180
+ A EA+ ++ + P++V I D + G S Y+ LIHRGD+ F
Sbjct: 121 FSVDLAVEAISMMTTDP----PILVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDF 180
Query: 181 TPVEVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMF 240
+ + EWD + LNYTSGTTSAPKGVV+ HRG F+ ++ ++ W + PVYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIF 240
Query: 241 HCNGWTFTWGIAARGGTNVCMRNTTAFDIFRNINLHHVSHMCCAPIVFRIILDADNGSPK 300
H NGW++ WGIAA GGTNVC+R A I+R I H V+HMC AP+V ++ + P
Sbjct: 241 HANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP- 300
Query: 301 RIGSLVNVMTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPGLVCEWQEKWNVLPAAEQ 360
+ VN++T GAPPPA +L + E++GF I+H YGLTE G + C W+ +WN LPA+++
Sbjct: 301 -LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDR 360
Query: 361 AKLKARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAF 420
A+LKARQG+ + ++DV + ++ SV +G T GEIV+RGSSIM GY KDP T A
Sbjct: 361 ARLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL 420
Query: 421 RNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMS 480
+NGWF TGDVGVIH DGYLEIKDRSKD+II+GGEN+SSVE+ETVLY +P V E AVVA
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARP 480
Query: 481 HPRWGESPCAFVAVHGGV----SEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQK 540
WGE+PCAFV++ G+ +E E+I +CRK + YMVPK V FV ELPKTSTGK+ K
Sbjct: 481 DVFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMK 540
Query: 541 FKLREQAKLFNVSK 543
F LRE AK ++
Sbjct: 541 FVLREIAKKMGTTR 548
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022923630.1 | 5.0e-288 | 85.24 | butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata] | [more] |
| XP_023513711.1 | 8.5e-288 | 85.24 | butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo] | [more] |
| KAG6602004.1 | 7.2e-287 | 85.07 | Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sorori... | [more] |
| XP_022990938.1 | 1.4e-285 | 84.55 | butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima] | [more] |
| XP_038906606.1 | 9.4e-279 | 82.53 | trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| I3PB36 | 3.2e-213 | 65.46 | Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 S... | [more] |
| Q9C8D4 | 1.6e-196 | 59.68 | Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11... | [more] |
| Q9SS01 | 1.1e-194 | 59.75 | Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 ... | [more] |
| Q9SS00 | 1.7e-193 | 59.82 | Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 ... | [more] |
| F4HUK6 | 8.9e-163 | 51.09 | Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1ECD9 | 2.4e-288 | 85.24 | butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
| A0A6J1JUR4 | 6.6e-286 | 84.55 | butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
| A0A5A7TXW0 | 1.0e-278 | 82.90 | Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194... | [more] |
| A0A1S3CIM1 | 1.0e-278 | 82.90 | butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC10350... | [more] |
| A0A0A0KT40 | 3.6e-276 | 82.26 | Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 G... | [more] |
