Spg008800 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008800
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionE3 ubiquitin-protein ligase RING1-like
Locationscaffold10: 34911649 .. 34912317 (-)
RNA-Seq ExpressionSpg008800
SyntenySpg008800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACTCTCTACGACTACCGCATATGGCAACCTGAAGAAGACGAAGAAGACGTTAATGTTACCATTTCCAATTCTCACTTGCTTCCCTGCGACTGTTTCCTTTTCCACCTTCACTTTCGACATGTTCTCCAGTCAAATCATCCTCAAGAATTGCAGAGGCTCCTGAATTCGTCTCTATCTTCGTTCTTGATACCTTGCGATGTCTTTTTCGCCAGTGGCTCCAGTATCCTGATCGCCGTTTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCGGCGGTGCCGGACGTTCTCTCCGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATACTTCCGTTGGCGGTGTCCGTTTTAGTCGTTTCGCCGTACAATGAACGCGAGGAAATTGGGCGAGATTTGCGGGAGGCGCAAGCGTTTAAAACGTTGCCGGCGAGTGAGTCGGCAATCGAAGGGCTGAAGAACGTGGAGATTGATGTCGGCGGAGAGTTGTTGATTGGTGACTGCAGAATATGTTTGGATGAACTGATGAATGGAGTGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGACTGCATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGCTGA

mRNA sequence

ATGGATACTCTCTACGACTACCGCATATGGCAACCTGAAGAAGACGAAGAAGACGTTAATGTTACCATTTCCAATTCTCACTTGCTTCCCTGCGACTGTTTCCTTTTCCACCTTCACTTTCGACATGTTCTCCAGTCAAATCATCCTCAAGAATTGCAGAGGCTCCTGAATTCGTCTCTATCTTCGTTCTTGATACCTTGCGATGTCTTTTTCGCCAGTGGCTCCAGTATCCTGATCGCCGTTTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCGGCGGTGCCGGACGTTCTCTCCGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATACTTCCGTTGGCGGTGTCCGTTTTAGTCGTTTCGCCGTACAATGAACGCGAGGAAATTGGGCGAGATTTGCGGGAGGCGCAAGCGTTTAAAACGTTGCCGGCGAGTGAGTCGGCAATCGAAGGGCTGAAGAACGTGGAGATTGATGTCGGCGGAGAGTTGTTGATTGGTGACTGCAGAATATGTTTGGATGAACTGATGAATGGAGTGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGACTGCATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGCTGA

Coding sequence (CDS)

ATGGATACTCTCTACGACTACCGCATATGGCAACCTGAAGAAGACGAAGAAGACGTTAATGTTACCATTTCCAATTCTCACTTGCTTCCCTGCGACTGTTTCCTTTTCCACCTTCACTTTCGACATGTTCTCCAGTCAAATCATCCTCAAGAATTGCAGAGGCTCCTGAATTCGTCTCTATCTTCGTTCTTGATACCTTGCGATGTCTTTTTCGCCAGTGGCTCCAGTATCCTGATCGCCGTTTTCTCCGGCACCGGCGTCGCCCAAGACTTCTTGGACGCGGCGGTGCCGGACGTTCTCTCCGTCGCGGCCGATATAGCATGCAATCCTCTTAATGCTGGCCGTCGGATACTTCCGTTGGCGGTGTCCGTTTTAGTCGTTTCGCCGTACAATGAACGCGAGGAAATTGGGCGAGATTTGCGGGAGGCGCAAGCGTTTAAAACGTTGCCGGCGAGTGAGTCGGCAATCGAAGGGCTGAAGAACGTGGAGATTGATGTCGGCGGAGAGTTGTTGATTGGTGACTGCAGAATATGTTTGGATGAACTGATGAATGGAGTGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGACTGCATCGTCAAATGGCTGGAGACGAGTCATTTGTGCCCGTTGTGTCGCTACGCAATGCCACTGAGCTGA

Protein sequence

MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSLSSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPLAVSVLVVSPYNEREEIGRDLREAQAFKTLPASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Homology
BLAST of Spg008800 vs. NCBI nr
Match: XP_023554199.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421.8 bits (1083), Expect = 3.7e-114
Identity = 206/224 (91.96%), Postives = 214/224 (95.54%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY+IWQPEE E+D+NVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL
Sbjct: 3   MDTLYDYQIWQPEEHEDDINVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SSFLIPCD FFASG SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+LPL
Sbjct: 63  SSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVLPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
           AVSVL VSPYNEREEIGR LRE+  QAFKT PASESAIEGLK VEIDVGGELLIG+CRIC
Sbjct: 123 AVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDELMNG+EVT+LPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. NCBI nr
Match: XP_022963379.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata] >XP_022963389.1 probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata])

HSP 1 Score: 420.2 bits (1079), Expect = 1.1e-113
Identity = 206/224 (91.96%), Postives = 213/224 (95.09%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY+IWQPEE E+DVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL
Sbjct: 3   MDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SSFLIPCD FFASG SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+LPL
Sbjct: 63  SSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVLPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
           AVSVL VSPYNEREEIGR LRE+  Q FKT PASESAIEGLK VEIDVGGELLIG+CRIC
Sbjct: 123 AVSVLAVSPYNEREEIGRVLRESSPQEFKTSPASESAIEGLKTVEIDVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDELMNG+EVT+LPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. NCBI nr
Match: XP_022990569.1 (probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima] >XP_022990570.1 probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima])

HSP 1 Score: 419.9 bits (1078), Expect = 1.4e-113
Identity = 206/224 (91.96%), Postives = 213/224 (95.09%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY+IWQPEE E+DVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL
Sbjct: 3   MDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SSFLIPCD FFASG SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+LPL
Sbjct: 63  SSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVLPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
           AVSVL VSPYNEREEIGR LRE+  QAFKT PASESAIEGLK VEIDVGGELLIG+CRIC
Sbjct: 123 AVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDELMNG+EVT+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. NCBI nr
Match: KAG6602099.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032803.1 E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 419.5 bits (1077), Expect = 1.8e-113
Identity = 206/224 (91.96%), Postives = 214/224 (95.54%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY+IWQPEE E+DVNVTISNSHLLPCDCFLFHLHFRHVLQSN+PQELQRLLNSSL
Sbjct: 3   MDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNNPQELQRLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SSFLIPCD FFASG SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+LPL
Sbjct: 63  SSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVLPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
           AVSVL VSPYNEREEIGR LRE+  QAFKT PASESAIEGLK VEIDVGGELLIG+CRIC
Sbjct: 123 AVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDELMNG+EVT+LPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. NCBI nr
Match: XP_038885922.1 (E3 ubiquitin-protein ligase RING1-like [Benincasa hispida])

HSP 1 Score: 408.3 bits (1048), Expect = 4.2e-110
Identity = 200/224 (89.29%), Postives = 210/224 (93.75%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY IWQP+E E D+NVT+SNSHLLPCDCFLFHLHFRHVLQSNHPQE   LLNSSL
Sbjct: 3   MDTLYDYHIWQPQEGEGDLNVTMSNSHLLPCDCFLFHLHFRHVLQSNHPQEFHLLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SSFLIPC+VF A+GSSILIAVFS TGVAQDFLD AVPDVLSVAADIACNPLNAGRRILPL
Sbjct: 63  SSFLIPCNVFLATGSSILIAVFSDTGVAQDFLDTAVPDVLSVAADIACNPLNAGRRILPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLRE--AQAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
           AVSVLVVSPYNEREEIGR LRE  AQ FKTLPASE+AIEGLKNV+IDVGGELLIG+CRIC
Sbjct: 123 AVSVLVVSPYNEREEIGRVLRESSAQPFKTLPASETAIEGLKNVDIDVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDELMNG+EVT+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELMNGMEVTRLPCAHVYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 4.5e-14
Identity = 40/113 (35.40%), Postives = 58/113 (51.33%), Query Frame = 0

Query: 109 NPLNAGRRILPLAVSVLVVSPYNEREEIGRDLREAQAFKTLPASESAIEGLKNVEIDVGG 168
           NP + G R LP  +    + P  E+        +   + T PAS+SAIE L  V I    
Sbjct: 158 NPSDQGFR-LPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSN 217

Query: 169 -ELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMP 221
                  C +C+D+   G E  Q+PC H YH+DC++ WLE  + CP+CR+ +P
Sbjct: 218 LNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELP 269

BLAST of Spg008800 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.0e-13
Identity = 32/78 (41.03%), Postives = 52/78 (66.67%), Query Frame = 0

Query: 146 FKTLPASESAIEGLKNVEIDVGGELL---IGDCRICLDELMNGVEVTQLPCAHFYHRDCI 205
           + T PAS+SAI+ L  V+  V  ++L   +  C +C+DE  +G +V Q+PC H +H+DC+
Sbjct: 186 YGTPPASKSAIDALPTVK--VTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCL 245

Query: 206 VKWLETSHLCPLCRYAMP 221
           + WLE  + CP+CR+ +P
Sbjct: 246 LPWLELHNSCPVCRFELP 261

BLAST of Spg008800 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.2e-12
Identity = 30/71 (42.25%), Postives = 42/71 (59.15%), Query Frame = 0

Query: 150 PASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETS 209
           PAS+SAIE L  VEI         +C +C +    G+E  ++PC H +H DCIV WL   
Sbjct: 190 PASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIR 249

Query: 210 HLCPLCRYAMP 221
           + CP+CR+ +P
Sbjct: 250 NSCPVCRFELP 260

BLAST of Spg008800 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.2e-12
Identity = 42/118 (35.59%), Postives = 62/118 (52.54%), Query Frame = 0

Query: 113 AGRRILPLAVSVLVVSPYNEREEIGRDLREAQAFKT-------LPASESAIEGLKNVEID 172
           +G R LP +VS +++    ER  +   L + +A  T        PAS+SAIE L  VEI 
Sbjct: 132 SGLRPLPDSVSEILMGSGFER--LLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEIS 191

Query: 173 ---VGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMP 221
              +G E    +C +C +      E  ++PC H +H DCIV WL   + CP+CR+ +P
Sbjct: 192 DCHIGSE---ANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELP 244

BLAST of Spg008800 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-11
Identity = 38/103 (36.89%), Postives = 58/103 (56.31%), Query Frame = 0

Query: 119 PLAVSVLVVSPYNEREEIGRDLREAQAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 178
           P    ++V+   +    + R L E++     PAS+++I+ +  VEID G E   G+C IC
Sbjct: 61  PFTQGMIVLEGSSGMNPLLRSLLESREEGRPPASKASIDAMPIVEID-GCE---GECVIC 120

Query: 179 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPL 222
           L+E  +   V ++PC H +H  CI KWL     CP+CRY MP+
Sbjct: 121 LEEWKSEETVKEMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPV 159

BLAST of Spg008800 vs. ExPASy TrEMBL
Match: A0A6J1HJY0 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC111463602 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 5.2e-114
Identity = 206/224 (91.96%), Postives = 213/224 (95.09%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY+IWQPEE E+DVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL
Sbjct: 3   MDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SSFLIPCD FFASG SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+LPL
Sbjct: 63  SSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVLPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
           AVSVL VSPYNEREEIGR LRE+  Q FKT PASESAIEGLK VEIDVGGELLIG+CRIC
Sbjct: 123 AVSVLAVSPYNEREEIGRVLRESSPQEFKTSPASESAIEGLKTVEIDVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDELMNG+EVT+LPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. ExPASy TrEMBL
Match: A0A6J1JSE1 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111487409 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 6.8e-114
Identity = 206/224 (91.96%), Postives = 213/224 (95.09%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY+IWQPEE E+DVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL
Sbjct: 3   MDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SSFLIPCD FFASG SILIAVFS TGVAQDFLDAAVPDVLSVAADIACNPLNAGRR+LPL
Sbjct: 63  SSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVLPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
           AVSVL VSPYNEREEIGR LRE+  QAFKT PASESAIEGLK VEIDVGGELLIG+CRIC
Sbjct: 123 AVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDELMNG+EVT+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. ExPASy TrEMBL
Match: A0A0A0KDU3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.0e-109
Identity = 198/224 (88.39%), Postives = 209/224 (93.30%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY IWQPEE E+D+NVTISNSHLLPC+CFLFHLHFRH+LQSNH Q+   LLNSSL
Sbjct: 3   MDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQFHYLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SSFLIPCDVFFA+ SSILIAVFSGTGVAQDFLD AVPDVLSVAADIACNPLNAGR ILPL
Sbjct: 63  SSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTILPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
           AVSVLVVSPYNER+EIGR LRE+  QAFKTLPASE AIEGLK VEIDVGGELLIG+CRIC
Sbjct: 123 AVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKKVEIDVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDELMNG+EVT+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. ExPASy TrEMBL
Match: A0A5A7V2F0 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G001370 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.1e-106
Identity = 192/224 (85.71%), Postives = 207/224 (92.41%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY IWQPEE E+D+NVTISNSHLLPC+CFLFHLHFRH+LQSNH Q+   LLNSSL
Sbjct: 3   MDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQSHYLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SS+LIPCDVFFA+ SSILIAVFSGTGVAQDFLD AVPDVLSVAADIACNPLNAGR ILPL
Sbjct: 63  SSYLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTILPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
            VSVLVVSPYNER+EIG+ LRE+  QAFKTLPASE AIE LKNVEI VGGELLIG+CRIC
Sbjct: 123 TVSVLVVSPYNERQEIGQVLRESSPQAFKTLPASEMAIEDLKNVEIGVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDEL+NG+E+T+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELVNGMEITRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. ExPASy TrEMBL
Match: A0A1S3CS28 (E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103504063 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.1e-106
Identity = 192/224 (85.71%), Postives = 207/224 (92.41%), Query Frame = 0

Query: 1   MDTLYDYRIWQPEEDEEDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNSSL 60
           MDTLYDY IWQPEE E+D+NVTISNSHLLPC+CFLFHLHFRH+LQSNH Q+   LLNSSL
Sbjct: 3   MDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQSHYLLNSSL 62

Query: 61  SSFLIPCDVFFASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRILPL 120
           SS+LIPCDVFFA+ SSILIAVFSGTGVAQDFLD AVPDVLSVAADIACNPLNAGR ILPL
Sbjct: 63  SSYLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTILPL 122

Query: 121 AVSVLVVSPYNEREEIGRDLREA--QAFKTLPASESAIEGLKNVEIDVGGELLIGDCRIC 180
            VSVLVVSPYNER+EIG+ LRE+  QAFKTLPASE AIE LKNVEI VGGELLIG+CRIC
Sbjct: 123 TVSVLVVSPYNERQEIGQVLRESSPQAFKTLPASEMAIEDLKNVEIGVGGELLIGECRIC 182

Query: 181 LDELMNGVEVTQLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 223
           LDEL+NG+E+T+LPCAH YHRDCIVKWLETSHLCPLCRYAMPLS
Sbjct: 183 LDELVNGMEITRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

BLAST of Spg008800 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.6e-14
Identity = 32/78 (41.03%), Postives = 52/78 (66.67%), Query Frame = 0

Query: 146 FKTLPASESAIEGLKNVEIDVGGELL---IGDCRICLDELMNGVEVTQLPCAHFYHRDCI 205
           + T PAS+SAI+ L  V+  V  ++L   +  C +C+DE  +G +V Q+PC H +H+DC+
Sbjct: 186 YGTPPASKSAIDALPTVK--VTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCL 245

Query: 206 VKWLETSHLCPLCRYAMP 221
           + WLE  + CP+CR+ +P
Sbjct: 246 LPWLELHNSCPVCRFELP 261

BLAST of Spg008800 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 3.9e-13
Identity = 33/75 (44.00%), Postives = 47/75 (62.67%), Query Frame = 0

Query: 150 PASESAIEGLKNVEIDVGGELLIGD---CRICLDELMNGVEVTQLPCAHFYHRDCIVKWL 209
           PASE  I  L +V+I    + L  D   C +C++E + G + T+LPC H YH+DCIV WL
Sbjct: 198 PASEPTINSLPSVKIT--PQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWL 257

Query: 210 ETSHLCPLCRYAMPL 222
             ++ CP+CR  +PL
Sbjct: 258 RLNNSCPICRRDLPL 270

BLAST of Spg008800 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 72.4 bits (176), Expect = 5.1e-13
Identity = 30/71 (42.25%), Postives = 42/71 (59.15%), Query Frame = 0

Query: 150 PASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKWLETS 209
           PAS+SAIE L  VEI         +C +C +    G+E  ++PC H +H DCIV WL   
Sbjct: 190 PASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIR 249

Query: 210 HLCPLCRYAMP 221
           + CP+CR+ +P
Sbjct: 250 NSCPVCRFELP 260

BLAST of Spg008800 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 5.1e-13
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 0

Query: 146 FKTLPASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKW 205
           + T PA++ A+E L  V+I+    LL   C +CLD+   G+E  ++PC H +H DC++ W
Sbjct: 200 YGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 259

Query: 206 LETSHLCPLCRYAMP 221
           LE    CP+CRY +P
Sbjct: 260 LELHSSCPVCRYLLP 270

BLAST of Spg008800 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 5.1e-13
Identity = 31/75 (41.33%), Postives = 47/75 (62.67%), Query Frame = 0

Query: 146 FKTLPASESAIEGLKNVEIDVGGELLIGDCRICLDELMNGVEVTQLPCAHFYHRDCIVKW 205
           + T PA++ A+E L  V+I+    LL   C +CLD+   G+E  ++PC H +H DC++ W
Sbjct: 200 YGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 259

Query: 206 LETSHLCPLCRYAMP 221
           LE    CP+CRY +P
Sbjct: 260 LELHSSCPVCRYLLP 270

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023554199.13.7e-11491.96probable E3 ubiquitin-protein ligase ATL45 [Cucurbita pepo subsp. pepo][more]
XP_022963379.11.1e-11391.96probable E3 ubiquitin-protein ligase ATL45 [Cucurbita moschata] >XP_022963389.1 ... [more]
XP_022990569.11.4e-11391.96probable E3 ubiquitin-protein ligase ATL45 [Cucurbita maxima] >XP_022990570.1 pr... [more]
KAG6602099.11.8e-11391.96E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038885922.14.2e-11089.29E3 ubiquitin-protein ligase RING1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
P0CH304.5e-1435.40E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q8LPN75.0e-1341.03E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9SNB67.2e-1242.25E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ... [more]
Q940T57.2e-1235.59E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Q9LQX21.2e-1136.89E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HJY05.2e-11491.96probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1JSE16.8e-11491.96probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0KDU31.0e-10988.39RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 P... [more]
A0A5A7V2F01.1e-10685.71E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CS281.1e-10685.71E3 ubiquitin-protein ligase RING1-like OS=Cucumis melo OX=3656 GN=LOC103504063 P... [more]
Match NameE-valueIdentityDescription
AT3G19950.13.6e-1441.03RING/U-box superfamily protein [more]
AT1G60360.13.9e-1344.00RING/U-box superfamily protein [more]
AT3G46620.15.1e-1342.25zinc finger (C3HC4-type RING finger) family protein [more]
AT3G13430.15.1e-1341.33RING/U-box superfamily protein [more]
AT3G13430.25.1e-1341.33RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 174..216
e-value: 0.0018
score: 13.5
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 175..215
e-value: 5.4E-7
score: 39.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 174..216
e-value: 2.1E-13
score: 50.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 175..216
score: 12.858103
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 137..221
e-value: 4.5E-19
score: 69.9
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 137..220
NoneNo IPR availablePANTHERPTHR15710:SF120F6A14.12 PROTEIN-RELATEDcoord: 137..220
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 174..216
e-value: 2.34911E-15
score: 65.7861
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 174..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008800.1Spg008800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding