Spg008721 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg008721
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	AMP dependent ligase
Location	scaffold10: 33182488 .. 33184151 (+)
RNA-Seq Expression	Spg008721
Synteny	Spg008721

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGATTTGAAGCCCAGCCCTGCAAATTCCTCTCCCTTCACGCCACTCGGCTTCCTCGACAGAGCCGCCGCCGTCTACGGCGACTCTCCCTCCATCATCTACAACTCCACCACCTACACCTGGTCCCAAACCCACCGTCGATGCCTTCAACTCGCCTCTTCACTCTCCTCCATCGGCAACCACAGAGGCCACGTCGTCTCCGTCGTCGCACCCAACACTCCCGCCATGTACGAGCTCCAATTCGCCGTTCCCATGGCTGGCGCCATCCTCAACAACGTCAATCTCCGCCTCGACGCTCGAACCATCTCCATCCTCCTCCGTCACAGCGAATCCAAACTCGTCTTCGTCGATCAGGCCTCCTGCGCCCTAATCTCCGAAGCCTTGGCCTTGTTCCCGCCGGAGGCCAAACGCCCGCCGCTCGTCCTCATCGCCGACGACGCTGCGGCAGAGGAATCCTCGTCCTCTGTTCCGATTTCGGCCGATTTTATCGGTTCCTATGAGGAAATGGTTCGGAAAGGGGATTCTGAGTTCCGGTGGATTCGGCCGGAGAGCGAGTGGGATCCCATCGTTCTGAATTACACCTCCGGAACGACGTCGTCGCCGAAGGGAGTGGTTCACTGTCACAGAGGAATTTTCTCAATAACTCTCGATTCGCTTCTCGAATGGGGAGTTCCGAAGCAATCGGTTTATTTATGGACCTTACCGATATTTCATGCCAACGGATGGAGTTACCCCTGGGGAATCGCCGCCGTAGGCGGCACAAATGTCTGTATTCGGAAATTCGATGCGGCGTTGATTTTTGCTCTAATTCGTCGTCATCGCGTGACGCACATGTGCGGAGCTCCGGTGGTCTTGAACATGCTAACGAACTCTACGGACAACCGGCCGCTTGATCGCACTGTCAAGATTCTCACCGCCGGAGCGCCGCCGCCTGCGGCGGTTCTGTTTCGCACCGAATCGTTGGGGTTCGATGTTAGTCATGGATACGGACTGACGGAGACGGCAGGGCTGGTGGTTTGCTGCTCGTGGAAGCCGCAGTGGAACCGGCTACCAGCGACGGAGCGGGCGCGGCTGAAGGCGAGGCAGGGAGTGAGGACGGCGGCGTTTTCGGCGGTTGATGTGGTGGATCCAGCGACCGGAAAGAGCGTGAAACGAGACGGAGCTTCGATCGGGGAGATCGTTCTGAGAGGCGGGTCGATTATGCTTGGGTATTTGAAAGATCCGGAATCGACGGCGAAATCGATGACCAGCGACGGGTGGTTCTTCACCGGAGACGTTGGGGTGATGCATCCAGATGGGTATTTGGAGATCAAGGACCGATCGAAGGATGTGATCATAAGCGGCGGAGAGAATTTAAGCAGCGTGGAGGTGGAATCGGTTCTATATACGAATCCGGCGGTGAATGAGGCGGCGGTGGTGGCGCGGCCGGACGAGTTCTGGGGGGAAACGCCGTGTGCGTTTGTGAGCTTGAGAGAAGGATTGACTTGGAAGCCGACGGAGGAGGAGATTATTGAGTATTGCAGAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTCTTCACGGCGGAGCTGCCGAAGACGTCGACCGGAAAGATCCAGAAGTTTGTGCTCAGGGAAATGGCCAAGTCGATGGGCCCTGGGCAAAAGAGCCGTTT

mRNA sequence

ATGGAAGATTTGAAGCCCAGCCCTGCAAATTCCTCTCCCTTCACGCCACTCGGCTTCCTCGACAGAGCCGCCGCCGTCTACGGCGACTCTCCCTCCATCATCTACAACTCCACCACCTACACCTGGTCCCAAACCCACCGTCGATGCCTTCAACTCGCCTCTTCACTCTCCTCCATCGGCAACCACAGAGGCCACGTCGTCTCCGTCGTCGCACCCAACACTCCCGCCATGTACGAGCTCCAATTCGCCGTTCCCATGGCTGGCGCCATCCTCAACAACGTCAATCTCCGCCTCGACGCTCGAACCATCTCCATCCTCCTCCGTCACAGCGAATCCAAACTCGTCTTCGTCGATCAGGCCTCCTGCGCCCTAATCTCCGAAGCCTTGGCCTTGTTCCCGCCGGAGGCCAAACGCCCGCCGCTCGTCCTCATCGCCGACGACGCTGCGGCAGAGGAATCCTCGTCCTCTGTTCCGATTTCGGCCGATTTTATCGGTTCCTATGAGGAAATGGTTCGGAAAGGGGATTCTGAGTTCCGGTGGATTCGGCCGGAGAGCGAGTGGGATCCCATCGTTCTGAATTACACCTCCGGAACGACGTCGTCGCCGAAGGGAGTGGTTCACTGTCACAGAGGAATTTTCTCAATAACTCTCGATTCGCTTCTCGAATGGGGAGTTCCGAAGCAATCGGTTTATTTATGGACCTTACCGATATTTCATGCCAACGGATGGAGTTACCCCTGGGGAATCGCCGCCGTAGGCGGCACAAATGTCTGTATTCGGAAATTCGATGCGGCGTTGATTTTTGCTCTAATTCGTCGTCATCGCGTGACGCACATGTGCGGAGCTCCGGTGGTCTTGAACATGCTAACGAACTCTACGGACAACCGGCCGCTTGATCGCACTAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTCTTCACGGCGGAGCTGCCGAAGACGTCGACCGGAAAGATCCAGAAGTTTGTGCTCAGGGAAATGGCCAAGTCGATGGGCCCTGGGCAAAAGAGCCGTTT

Coding sequence (CDS)

ATGGAAGATTTGAAGCCCAGCCCTGCAAATTCCTCTCCCTTCACGCCACTCGGCTTCCTCGACAGAGCCGCCGCCGTCTACGGCGACTCTCCCTCCATCATCTACAACTCCACCACCTACACCTGGTCCCAAACCCACCGTCGATGCCTTCAACTCGCCTCTTCACTCTCCTCCATCGGCAACCACAGAGGCCACGTCGTCTCCGTCGTCGCACCCAACACTCCCGCCATGTACGAGCTCCAATTCGCCGTTCCCATGGCTGGCGCCATCCTCAACAACGTCAATCTCCGCCTCGACGCTCGAACCATCTCCATCCTCCTCCGTCACAGCGAATCCAAACTCGTCTTCGTCGATCAGGCCTCCTGCGCCCTAATCTCCGAAGCCTTGGCCTTGTTCCCGCCGGAGGCCAAACGCCCGCCGCTCGTCCTCATCGCCGACGACGCTGCGGCAGAGGAATCCTCGTCCTCTGTTCCGATTTCGGCCGATTTTATCGGTTCCTATGAGGAAATGGTTCGGAAAGGGGATTCTGAGTTCCGGTGGATTCGGCCGGAGAGCGAGTGGGATCCCATCGTTCTGAATTACACCTCCGGAACGACGTCGTCGCCGAAGGGAGTGGTTCACTGTCACAGAGGAATTTTCTCAATAACTCTCGATTCGCTTCTCGAATGGGGAGTTCCGAAGCAATCGGTTTATTTATGGACCTTACCGATATTTCATGCCAACGGATGGAGTTACCCCTGGGGAATCGCCGCCGTAGGCGGCACAAATGTCTGTATTCGGAAATTCGATGCGGCGTTGATTTTTGCTCTAATTCGTCGTCATCGCGTGACGCACATGTGCGGAGCTCCGGTGGTCTTGAACATGCTAACGAACTCTACGGACAACCGGCCGCTTGATCGCACTAGGGAAGCTGCCGCGGTTTATGGTGCCGAAGACGGTGGTCTTCACGGCGGAGCTGCCGAAGACGTCGACCGGAAAGATCCAGAAGTTTGTGCTCAGGGAAATGGCCAAGTCGATGGGCCCTGGGCAAAAGAGCCGTTT

Protein sequence

MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIGNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFRWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPLDRTREAAAVYGAEDGGLHGGAAEDVDRKDPEVCAQGNGQVDGPWAKEPF

Homology

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BLAST of Spg008721 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 515.4 bits (1326), Expect = 3.9e-142
Identity = 248/302 (82.12%), Postives = 275/302 (91.06%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD PSIIYNSTTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RG VVSVV+PNT AMYEL FAVPMAGA+LN +NLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIGSYEEMVRKGDSEF 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS  SSV   ADF+ SYEEMV+KGD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 RWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++TLDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPL 300
           HANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNS DNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

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BLAST of Spg008721 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 514.6 bits (1324), Expect = 6.6e-142
Identity = 248/306 (81.05%), Postives = 275/306 (89.87%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD PSIIYNSTTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RG VVSVV+PNTPAMYEL FAVPMAGA+LN +NLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS------SSVPISADFIGSYEEMVRKG 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS      SSV   ADF+ SYEEMV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSEFRWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWT 240
           D  F+WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++TLDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPIFHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTD 300
           LP+FHANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNS D
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

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BLAST of Spg008721 vs. NCBI nr
Match: XP_008463174.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046220.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18976.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 514.2 bits (1323), Expect = 8.6e-142
Identity = 248/302 (82.12%), Postives = 272/302 (90.07%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANS P TP+GF+ RAA +YGDS SIIYNSTTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RGHVVSV+APNTPAMYEL FAVPM GAILN +NLRLD RTISILLRHSESKL+FVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIGSYEEMVRKGDSEF 180
           SCALI EALALFPPE KRP LVLIADDAA ++SS  S V I  +F+ SYE+MV++GDS+F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 RWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSLLEWGVPKQSVYLWTLP+F
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPL 300
           H NGWS+PWGIAAVGGTNVCIRKFDA LIF+LIRRH VTH+CGAPVVLNMLTNS DN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

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BLAST of Spg008721 vs. NCBI nr
Match: KAG6601999.1 (putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 509.6 bits (1311), Expect = 2.1e-140
Identity = 245/299 (81.94%), Postives = 264/299 (88.29%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP PANSSP TP+GFL+RAA +YGDSPSIIY  TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAAIIYGDSPSIIYGHTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RGHVVSV++PNTP MYEL FAVPM GAILN +N RLDARTISILL HSESKL+FVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFRW 180
            C LI  AL LFPPE  RP LVLIADDA AEESSSSV I  DF+GSYEEM+RKGDS+FRW
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLIADDAVAEESSSSVLIDGDFLGSYEEMIRKGDSDFRW 180

Query: 181 IRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP SEWDPI LNYTSGTTSSPKGVVHCHRG F IT+D LLEWGVPKQSVYLWTLP+FHA
Sbjct: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPLD 300
           NGWS+ WG+AAVGGTN+CIRKFDAAL+++LIRRH VTH+CGAPVVLNMLTNS D RPLD
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAALVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 299

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BLAST of Spg008721 vs. NCBI nr
Match: XP_023550909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.8 bits (1309), Expect = 3.6e-140
Identity = 246/306 (80.39%), Postives = 274/306 (89.54%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD  SIIY+STTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCRSIIYDSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RG VVSVV+PNT AMYEL FAVPMAGA+LN +NLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS------SSVPISADFIGSYEEMVRKG 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS      SSV   ADF+ SYEEMV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVLNRADFVDSYEEMVQKG 180

Query: 181 DSEFRWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWT 240
           D  F+WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++TLDSL+EWGVPK+SVYLWT
Sbjct: 181 DPLFQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWT 240

Query: 241 LPIFHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTD 300
           LP+FHANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNS D
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

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BLAST of Spg008721 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 7.1e-123
Identity = 206/301 (68.44%), Postives = 253/301 (84.05%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP PA+SSP TPLGFL+RAA VYGD  S++Y++ +YTWSQTHRRCL LASS++S+G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
              GHVVSV+APN P MYEL FAVPMAGAILN VNLRLDARTISILL HSESKL+FVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFRW 180
           S  LI EA+ALFP +A  P LV +AD++   ES +S  +  +F  SY++++ +GD +F+W
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADES---ESGNSSELGKEFFCSYKDLIDRGDPDFKW 180

Query: 181 IRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFHA 240
           + P+SEWDP++LNYTSGTTSSPKGVVHCHRGIF +T+DSL++WGVPKQ VYLWTLP+FHA
Sbjct: 181 VMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHA 240

Query: 241 NGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPLDR 300
           NGWSYPWG+AAVGGTN+C+RKFD+ +I+ +I+RH VTHMCGAPVVLNML+N+  + PL  
Sbjct: 241 NGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKT 298

Query: 301 T 302
           T
Sbjct: 301 T 298

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BLAST of Spg008721 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.0e-113
Identity = 198/300 (66.00%), Postives = 239/300 (79.67%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSS-I 60
           ME+LKP  ANS P TPLGFL+RAA VYGD  S++Y+  +YTWSQTH RCL++AS + S +
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           G  +G VVSVVAPN PAMYEL FAVPMAG +LNN+N RL+A TIS++LRHSESKLVFVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSE-F 180
            S  L+ +A++LFP     P LVLIAD+   E  + +V     F+  YE++V KGD + F
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGE--NLTVEDREHFVCCYEDLVEKGDDKTF 180

Query: 181 RWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIF 240
           +W+RP SEWDPIVLNYTSGTTSSPKGVVH HR +F +TLDSL++WGVPKQ VYLWTLP+F
Sbjct: 181 KWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPL 299
           HANGW Y WGIAAVGGTN+C+R+FD  +IF LIRRHRVTHMC AP+VLNML+NS +  PL
Sbjct: 241 HANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPL 298

[Close]

BLAST of Spg008721 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.3e-111
Identity = 194/301 (64.45%), Postives = 239/301 (79.40%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNS-TTYTWSQTHRRCLQLASSLSSI 60
           ME +KP  ANS P TP+GFL+RAA VYGD  SI+Y S T YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           G  R  VVSV++PNTPAMYELQFAVPM+GAILNN+N RLDART+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFR 180
            S  L  EA+++   +   PP+++   D   E   + V     F  +Y++++ +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTD---PPILVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFH 240
           WIRPESEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLPIFH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPLD 300
           ANGWSYPWGIAAVGGTNVC+RKFDA LI+ LIR H VTHMCGAPVVLNML+ + + +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 298

[Close]

BLAST of Spg008721 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.8e-110
Identity = 191/300 (63.67%), Postives = 241/300 (80.33%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNS-TTYTWSQTHRRCLQLASSLSSI 60
           ME++KP  ANS P TP+GFL+RAA VYGD  SI+Y S T YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           G  R  VVSV++PNTPAMYELQFAVPM+GAILNN+N RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFR 180
            S  L  EA+++   +   PP++++  D   E   + V   + F  +Y++++ +GD  F+
Sbjct: 121 FSVDLAVEAVSMMTTD---PPILVVIADKEEEGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFH 240
           WIRPESEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLPIFH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPLD 300
           +NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTHMCGAPVVLNML+ + +++PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 297

[Close]

BLAST of Spg008721 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.1e-102
Identity = 185/296 (62.50%), Postives = 226/296 (76.35%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIY-NSTTYTWSQTHRRCLQLASSLSSI 60
           MEDLKPS ANS P T LGFL+RAA VYGD  SI+Y NST YTW +T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           G  R  VVSV++ NTP MYELQF+VPM+GAILNN+N RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFR 180
               L  EA+ +       PP++++  +   EE  + V   + F   Y +++ +G+ +F+
Sbjct: 121 FYSDLAVEAITML----LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFH 240
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRGIF +TLDSL +W VPK  VYLWTLPIFH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDN 296
           ANGW+YPWGIAAVGGTNVC+RK  A  I+ LIR H VTHM GAP+VL +L+ S ++
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQES 292

[Close]

BLAST of Spg008721 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.9e-142
Identity = 248/302 (82.12%), Postives = 275/302 (91.06%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD PSIIYNSTTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RG VVSVV+PNT AMYEL FAVPMAGA+LN +NLRLDARTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIGSYEEMVRKGDSEF 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS  SSV   ADF+ SYEEMV+KGD  F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 181 RWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++TLDSL+EWGVPK+SVYLWTLP+F
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPL 300
           HANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNS DNRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

[Close]

BLAST of Spg008721 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.2e-142
Identity = 248/306 (81.05%), Postives = 275/306 (89.87%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANSSP TP+GFL+RAA VYGD PSIIYNSTTYTW++THRRCLQLASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RG VVSVV+PNTPAMYEL FAVPMAGA+LN +NLRLD+RTIS++LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS------SSVPISADFIGSYEEMVRKG 180
           SC LIS+ALALFPPE KRPPL+LIAD+AAAE+SS      SSV   ADF+ SYEEMV+KG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 181 DSEFRWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWT 240
           D  F+WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++TLDSL+EWGV KQSVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 241 LPIFHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTD 300
           LP+FHANGWS+PWGIAAVGGTNVCIRKFDAA IFALI R+ VTH+CGAPVVLNMLTNS D
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

[Close]

BLAST of Spg008721 vs. ExPASy TrEMBL
Match: A0A5D3D623 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00690 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 4.2e-142
Identity = 248/302 (82.12%), Postives = 272/302 (90.07%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANS P TP+GF+ RAA +YGDS SIIYNSTTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RGHVVSV+APNTPAMYEL FAVPM GAILN +NLRLD RTISILLRHSESKL+FVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIGSYEEMVRKGDSEF 180
           SCALI EALALFPPE KRP LVLIADDAA ++SS  S V I  +F+ SYE+MV++GDS+F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 RWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSLLEWGVPKQSVYLWTLP+F
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPL 300
           H NGWS+PWGIAAVGGTNVCIRKFDA LIF+LIRRH VTH+CGAPVVLNMLTNS DN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

[Close]

BLAST of Spg008721 vs. ExPASy TrEMBL
Match: A0A1S3CIL0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501381 PE=4 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 4.2e-142
Identity = 248/302 (82.12%), Postives = 272/302 (90.07%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP+PANS P TP+GF+ RAA +YGDS SIIYNSTTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RGHVVSV+APNTPAMYEL FAVPM GAILN +NLRLD RTISILLRHSESKL+FVDQA
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESS--SSVPISADFIGSYEEMVRKGDSEF 180
           SCALI EALALFPPE KRP LVLIADDAA ++SS  S V I  +F+ SYE+MV++GDS+F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 RWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIF 240
           +WIRP SEWDPIVLNYTSGTTSSPKGVVHCHRGIF++ LDSLLEWGVPKQSVYLWTLP+F
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPL 300
           H NGWS+PWGIAAVGGTNVCIRKFDA LIF+LIRRH VTH+CGAPVVLNMLTNS DN PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

[Close]

BLAST of Spg008721 vs. ExPASy TrEMBL
Match: A0A6J1E3B0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430201 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 8.7e-140
Identity = 243/299 (81.27%), Postives = 263/299 (87.96%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNSTTYTWSQTHRRCLQLASSLSSIG 60
           MEDLKP PANSSP TP+GFL+RAA +YGDSPSIIY  TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  NHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQA 120
             RGHVVSV++PNTP MYEL FAVPM GAILN +N RLDARTISILL HSESKL+FVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 SCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFRW 180
            C LI  AL LFPPE  RP LVLIADDA AEESSSSV I  +F+GSYEEM+RKGDS+FRW
Sbjct: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180

Query: 181 IRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFHA 240
           IRP SEWDPI LNYTSGTTSSPKGVVHCHRG F IT+D LLEWGVPKQSVYLWTLP+FHA
Sbjct: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPLD 300
           NGWS+ WG+AAVGGTN+CIRKFDA L+++LIRRH VTH+CGAPVVLNMLTNS D RPLD
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 299

[Close]

BLAST of Spg008721 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 404.4 bits (1038), Expect = 8.9e-113
Identity = 194/301 (64.45%), Postives = 239/301 (79.40%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNS-TTYTWSQTHRRCLQLASSLSSI 60
           ME +KP  ANS P TP+GFL+RAA VYGD  SI+Y S T YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           G  R  VVSV++PNTPAMYELQFAVPM+GAILNN+N RLDART+S+LLRH  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFR 180
            S  L  EA+++   +   PP+++   D   E   + V     F  +Y++++ +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTD---PPILVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFH 240
           WIRPESEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLPIFH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPLD 300
           ANGWSYPWGIAAVGGTNVC+RKFDA LI+ LIR H VTHMCGAPVVLNML+ + + +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 298

[Close]

BLAST of Spg008721 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.3e-111
Identity = 191/300 (63.67%), Postives = 241/300 (80.33%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYNS-TTYTWSQTHRRCLQLASSLSSI 60
           ME++KP  ANS P TP+GFL+RAA VYGD  SI+Y S T YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           G  R  VVSV++PNTPAMYELQFAVPM+GAILNN+N RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFR 180
            S  L  EA+++   +   PP++++  D   E   + V   + F  +Y++++ +GD  F+
Sbjct: 121 FSVDLAVEAVSMMTTD---PPILVVIADKEEEGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFH 240
           WIRPESEWDP+VLNYTSGTTS+PKGVVHCHRGIF +++DSL++W VPK  VYLWTLPIFH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRPLD 300
           +NGW+ PWGIAAVGGTNVC+RKFDA LI+ LIR H VTHMCGAPVVLNML+ + +++PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 297

[Close]

BLAST of Spg008721 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 374.8 bits (961), Expect = 7.6e-104
Identity = 185/296 (62.50%), Postives = 226/296 (76.35%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIY-NSTTYTWSQTHRRCLQLASSLSSI 60
           MEDLKPS ANS P T LGFL+RAA VYGD  SI+Y NST YTW +T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFVDQ 120
           G  R  VVSV++ NTP MYELQF+VPM+GAILNN+N RLDART+S+LLRH ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 ASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSEFR 180
               L  EA+ +       PP++++  +   EE  + V   + F   Y +++ +G+ +F+
Sbjct: 121 FYSDLAVEAITML----LNPPILVLIANEEEEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPIFH 240
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRGIF +TLDSL +W VPK  VYLWTLPIFH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDN 296
           ANGW+YPWGIAAVGGTNVC+RK  A  I+ LIR H VTHM GAP+VL +L+ S ++
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQES 292

[Close]

BLAST of Spg008721 vs. TAIR 10
Match: AT1G21530.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 372.9 bits (956), Expect = 2.9e-103
Identity = 184/301 (61.13%), Postives = 234/301 (77.74%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYN-STTYTWSQTHRRCLQLASSL--S 60
           ME L P P+NS+P T LGFLDRAA+VYGD PSI++  +T +TWS+TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFV 120
           SIG  +G VVSVV PN P++YELQFAVPM+GAILNN+N RLDA  +S+LLRHSES+LVFV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSE 180
           D  S +L+ EA++LF  + ++P LVL+ DD   + SS+S     DF+ +YEE++ +G+S 
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLLDDDQENDSSSAS-----DFLDTYEEIMERGNSR 180

Query: 181 FRWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPI 240
           F+WIRP++EW P+VLNYTSGTTSSPKGVV  HR IF +T+ SLL+W VP + VYLWTLP+
Sbjct: 181 FKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTLPM 240

Query: 241 FHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRP 299
           FHANGW Y WG AAVG TN+C R+ DA  I+ LI +H VTHMC AP+VLNML N   + P
Sbjct: 241 FHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLSTP 295

[Close]

BLAST of Spg008721 vs. TAIR 10
Match: AT1G21530.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 372.9 bits (956), Expect = 2.9e-103
Identity = 184/301 (61.13%), Postives = 234/301 (77.74%), Query Frame = 0


Query: 1   MEDLKPSPANSSPFTPLGFLDRAAAVYGDSPSIIYN-STTYTWSQTHRRCLQLASSL--S 60
           ME L P P+NS+P T LGFLDRAA+VYGD PSI++  +T +TWS+TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGNHRGHVVSVVAPNTPAMYELQFAVPMAGAILNNVNLRLDARTISILLRHSESKLVFV 120
           SIG  +G VVSVV PN P++YELQFAVPM+GAILNN+N RLDA  +S+LLRHSES+LVFV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQASCALISEALALFPPEAKRPPLVLIADDAAAEESSSSVPISADFIGSYEEMVRKGDSE 180
           D  S +L+ EA++LF  + ++P LVL+ DD   + SS+S     DF+ +YEE++ +G+S 
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLLDDDQENDSSSAS-----DFLDTYEEIMERGNSR 180

Query: 181 FRWIRPESEWDPIVLNYTSGTTSSPKGVVHCHRGIFSITLDSLLEWGVPKQSVYLWTLPI 240
           F+WIRP++EW P+VLNYTSGTTSSPKGVV  HR IF +T+ SLL+W VP + VYLWTLP+
Sbjct: 181 FKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTLPM 240

Query: 241 FHANGWSYPWGIAAVGGTNVCIRKFDAALIFALIRRHRVTHMCGAPVVLNMLTNSTDNRP 299
           FHANGW Y WG AAVG TN+C R+ DA  I+ LI +H VTHMC AP+VLNML N   + P
Sbjct: 241 FHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLSTP 295

The following BLAST results are available for this feature:

BLAST of Spg008721 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022939278.1	3.9e-142	82.12	probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057....	[more]
XP_022993747.1	6.6e-142	81.05	probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima]	[more]
XP_008463174.1	8.6e-142	82.12	PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA004...	[more]
KAG6601999.1	2.1e-140	81.94	putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma ...	[more]
XP_023550909.1	3.6e-140	80.39	probable acyl-activating enzyme 5, peroxisomal [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg008721 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IQQ5	7.1e-123	68.44	2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1	[more]
M4ISH2	3.0e-113	66.00	Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1	[more]
Q9FFE6	1.3e-111	64.45	Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9FFE9	1.8e-110	63.67	Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 S...	[more]
Q9LQS1	1.1e-102	62.50	Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 S...	[more]

BLAST of Spg008721 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FL76	1.9e-142	82.12	probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JTQ2	3.2e-142	81.05	probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5D3D623	4.2e-142	82.12	Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3CIL0	4.2e-142	82.12	probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1E3B0	8.7e-140	81.27	probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of Spg008721 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16370.1	8.9e-113	64.45	acyl activating enzyme 5	[more]
AT5G16340.1	1.3e-111	63.67	AMP-dependent synthetase and ligase family protein	[more]
AT1G75960.1	7.6e-104	62.50	AMP-dependent synthetase and ligase family protein	[more]
AT1G21530.1	2.9e-103	61.13	AMP-dependent synthetase and ligase family protein	[more]
AT1G21530.2	2.9e-103	61.13	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 21..296 e-value: 4.0E-52 score: 177.2
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 3..327 e-value: 7.9E-57 score: 194.6
None	No IPR available	PANTHER	PTHR43859	ACYL-ACTIVATING ENZYME	coord: 2..296
None	No IPR available	PANTHER	PTHR43859:SF50	LONG-CHAIN ACYL-COA SYNTHETASE	coord: 2..296
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 11..311
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 192..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg008721.1	Spg008721.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016874	ligase activity