Spg008711 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg008711
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionmetal transporter Nramp5-like
Locationscaffold10: 35098624 .. 35106701 (-)
RNA-Seq ExpressionSpg008711
SyntenySpg008711
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGACGGGATGAAAGCACACGTTGAGACGGCTAGATCCGACGAGATCGTGAGAGCAGAAACCAGATTTGGCGTCGTCGGAGCCGACAAGGACTATGTCGGAGAAGAAGAACTTTTGAGGTTGTCGGCGGCCGGCGTTAGCGGTGGCTGTGGCCGGCGACGGCTGACGGAAGAAGAAGGAAGAAGGCCGACGGAGAAGAAGAAGAATTGGAGGCCGAAGGAGAAGAAGAAAAAGGTAGAAGAAAAGAGAAAAGAGAGAGAAAAAAGAATAAAAGAAGAATATTTTTTTTTTAATTTAATATTATTTTTATTTAAAAAAAATATATATTTTTTTAAATAAAAAATAGGTGCCACGTCATCAATTCGTCAGTAATTAACGGAAAAATGGATGGAAGGACCAAAATGAAACGAAATCCAAACCTCAGGGACTAAAATGTCGAGTTTGAAAACTCAGGGACCAAAATGAAACCTAACCCAAACCTCAGGGACCAAAAGTGTATTTTTCCCAAACTTTAAACTCCCCGCTTAGTAGAGATTAAAAGTTCTCATTATTTGTATTATGTTCACTTTAGGATATTTATTGACACTTTAATGAAAATAAACTTTGACAAAGTATTAAACTTTTAAATAAATTCAAGCTTATGAACTTTTAAATTTATATCTAATAGATTCCTAAATTGTGAGAGTGTTTAAATAGTTTGTAGGTATTAAACTTTTTTCTTCCGGTGTAATAAATCTTTGAATTGTAATTTTTTTTTATTAGGTTTATAAGCTTTCAATTTTATATCTAATAAATCATTGACTGACCACTTATTCAACTATTTTTAAAATTTATGAACATATTAAATAAAAATTGAAAATATAGAAAGATACATTAGGTTTGTTTTCTTCTAATTTCTTTACAATGATTTTCATATATTTGTCAATGACACATTTGAATATTTAGCCAAATTCCAAAAAAGAAAAAAAAAACTTTTGAAAACTACTCTTTTTTTTTTTTATTAACTTTCAAAACTTTGACTCTTGGTTCTTGAAAATGCGAGAAAATGACCAAACAAATAATTTTTTTTTTAAATAACAAAACAAAGAAACTTATTGATGGAATTAGGGTCTGTTTGAAATACATTCTAATTTAAATGTTTTTCAAGAATACATTTTTTCAAAAACATTTTGTATAAAAATTGTTTAGAATTTAAACCCTCATATGTTTGGTTACATTTCCTCATAGGTGTTTTTATACACAAGTTATTGTATACTTAAAAACTCTTTTTAAAAAAGTTGGTTTCGAGTGTTTCTCTTAAAATAATTTATTTCCCAATCTCATTTTCTAATAAGTCATTTTTAGTGGTTGTCAAACACTAGAATTTTTTTCAAAATGATTTATTTTCAAAATTAAACACTTGAAAAGTTAAACAAAACACACCATTTAGTGTTTCTAAGCTTAATTTCCACAAAAAAAAAAAATGGTTATCAAAGAGGACCAATAAGTTTGATACTTTTTAAAATTTAAAATCTTAATTAATTACTAGACACAAATTTAAAAGTTGGACCTGCAAGTTTAGAAAAATTATGTACTAAACTACTTATAATTTAATTTTATTCATATATTTTGTTAACATTTTAACTGTATAGTATAGAACTAGAAAAAAAACACATTTAAACTATACAATAAGAATATCGACCAATTCTCTGTATCATAAATATATGAGGAATTGTCAATAAAAACATAGTTCAATTGACATAAAGTCTCATAAGTATAACATCTATGGTTCAAATTTCTTTAAAAAAAAAAATTGTGTAAAAATGTGATTGACACATCCACGAGAATTTAATACACTCTGGACGGCTGGCTAAGCTGTCGTTATGCAGAGTCACTAACTCTATAAATACTTTAACTGCTGATTCTCTTATATCATCGTCAACTCGGCAGCCACATTGAAGAGAGAGAGAGAAGAAATGGCAAGTCGGCAGAAGGAAGCTCAGATGAGTACGGCGGCGACACCGTCTTTTGGTGGTGGCAGCAACCGCATAGCGGCAGTGACGACCACTCCGGCCAACGCCGTCAAGCCACAAAATCACCATGAAGAACAAACCACCACGCATCAGGTTGCTCTATAAGAAAGAAGAAAAGAAAATTAAAGTGAAAAAACAAAAAATATTTTCTTTTATTTTAAAATTTTTAATTTTAAGCTAATTTTGATTATTCAGAAACCTGGATGGAGAAAATTTCTTTCACACGTTGGCCCTGGCTTCCTCGTCTCATTGGCTTACCTTGATCCCGGCAACTGTAAGCTCCGTGTATATTTGGAAGTGATTTTGAAATAACTAAAATCACTTTTGTTATATTCAAATTAAAATTATCCAGCAAGGAACATACTTCTAATTATTTAAGATCTATTCTAAGTGAGTGTGAAAGAAATATTATTTCCATATATACTTCTGATTTTAGCTTTTTAAAATATTTTATTGATATCATTTTTTTTTTTCAAAAGAAAATAATTTTACGAAGTTTTAATCTGACAGGCTATATTGTGTTTTTTTGTTAATTTTTCAGTGGAAACCGATTTGCAGGCTGGAGCTAATCACGGATACGAGGTAATCAAAGTCAACAAATTATATATAATAGAAAAATAATATCAAGTTCAAGAACAAAATTTTTCAAAAAAAAAAAAAAAAGGTTCAAGAACAAAAAGAAAAAAAAATTATATATTATTAGAACACTAATTATACGTAATTTACCACTAGAAAATAAAAAGAGGCCGACCAAATCGGCTGAATGAAGTGGGTTTGATTTTATCAACTTTGAGATTAAATAAATTATATAAATGGATTCGATATTATAAGAAAATCATTTTTATTTAATAAAATGTTTTTTGTTCAATGGCTGGCAGCTTTTGTGGGTGGTGCTTATCGGATTAATATTTGCGCTCATAATCCAATCTCTCGCTGCAAATCTCGGCGTAAGCACTGGTAAGTAAGAAACTAAAATAAACTGAAAAAGAATTAATAAGATTAATTGACCTCGCAAATAATTTATGGTTATTGATTACTTTATTAATTAACGCTAATTAATTAATTACTTTTTTTTAATTTTATTTTTCAAAGGAAAACACCTTTCAGAAGTTTGCAAAGCCGAATATCCCAAATTTGTGAAGTACTTCCTTTGGTTGCTGGCTGAAGTTGCTGTAATAGCCGCCGATATTCCAGAAGGTATTATTATTATTATTGTATAATTTATTTTATTTATTTTTAACAATTTTGTAATCGTTTTAGATGTTAAAATAATTAATCTGTCTTATATTTCATAATTTTGTGTTTGGTTGCAGTGATAGGAACGGCCTTTGCCCTGAATATACTATTCCATATTCCAGTTTGGGCGGGAGTTCTTCTCACCGGATTAAGCACCCTCTTGCTCCTTGGACTCCAGAGATATGGGGTAAATTATTTAATATTTAAATTATCTTTTAGTCCTCAAACTTTTTATCCTTATTTTAGTTCGACCCTAAACTTTTTAATATTCTATTATTCTTAGATTTCGACTTTACATTCAAATGAGGTGGTTTTGTGATAATTAGCGATAACATATTAAAATTTCTCTTAAAAAATTATTGGTCAAATTACAAGTTTAATCCATGAACATTGTGTCAATTTGATCTCTGAATTTTAAAAAGTGTCAAATAAATAATCGAACTTTCAATTTTGCGTCGAATAGGTCTTTGAACTTTAAAAAGTATCCAATAGGTCCTTACACTTTTACTTTTATGTCCAATAGACTTTTCAACTTTCAATTTTGTACTTAATAACTTCTGGACCTATTTGATATATTTTTTAAAAAAAATTTAGGCCTACTAAATACAAAATTAGAAGTTTAGGGTCTCAATAAGCATAAAATTCAATTGTATATCTTTTTTTCTTAGAAGAATTCAATTGTATATCTAATAGATAGAATTTTAATTAAAATCTATAATGTGTTAGTTAGGGACCTATTAGATGAAAGTTTATGGACTTATTATACATTTTTAAAGTTCAGAGACCTATCAAAGACAAAATTGAAAGTTTAAGCATTTATCAGACACTTTTTAAAGTTTAGAAACATAATCTCGATACAACTTTGAAAATTCAAAAGACTAAACTTATAGTAAAACCTCTTCTAAAAAAAGAAAAAAAAAATGGTAGTAAAACCAAAATTATTTTATTCTGCACTGTTATTTTATGATTAATGAGTAAAGAGTCAAATGAAACTGGCATAAAGTTGCATAAACTAGCATAATTTTTTGCCAACAAGAGCTTAGCTCAGCTGGCATAAAGTTGTACTAATAATTATAAGGTCTGTGGTTCGAATCTTTCTCTCTACATATTGTCAAAAAAAACTAAAATAGATCAGAAAAAGACAGAAATTAAAATAGAAAAAGGAAAGTAATATTTAAATTAAAGATGGTTTTAATTATTTTGTAATTTGCAGGCGAGGAAGCTGGAATTGCTGATAGCAGTTTTGGTATTCGTAATGGCGGCGTGTTTCTTCGGGGAAATGAGTTACGTTAAGCCGCCGGCGACCGGCGTTTTGAAAGGAATGTTTGTGCCGAAACTCAACGGCCAAGGAGCCACCGCCGACGCCATTGCTCTCCTCGGCGCTCTCGTTATGCCGTACGATTCTAATTTTTGATCAACCGACATGTCGGAACTTGGCATACATTTTATTATTATTATTATTATTATTTTTTTTTTTCTGAAATCTGAATTTATGAAATATTTTGGAATTGAATTTGTGGAACAGGCACAATCTCTTTCTTCATTCGGCTCTCGTGCTCTCTAGGAAGGTGCCAAATTCCGTCCGCGGTATCAACGTATGTTTTCTATTACAGCGCTTATTAATTTAATATTATTATTTATTATTACATGGAAAATTTCGTCAATTTAAGTATAGTTCCTTTACTTAAGGTCGGAGTTTTAAATCTTTATTATTACATACGATTGAAAAAAAACAAAATAAATAGAATAAAAGTATCTTTATTTCAATGTTCTTTAAACTTTAGCACACTACTTTGAAATTTACCCCAAAGCGAGGATGAATGATATATTTTCATTAGTGGTAATCTTCTATGTCTTTCATTTTTCCATGTGCTCTCAAAATGGTCCAACTCTTAAATTTTAGTCCGTGTACTTTCAATATTTTTTTTTTCGTATACGTAGAAAAAAATATGGTTTAAATCATTAAATAATATTCTTTTTTATTTGTTTAATTCAACTTTAATTTTAAATTACTCTCTTCTAAAAAATAATAATAATAAGGAAAAAATACACTTTTAGTCCTTGAGATTTGGGGTAGGTTTTATTTTGGTCCTTCGGTTTTCAAACTCAACAATTTAGCCCTTAAGGTTTGGATTTCGTTACACTTTTGGTCCCTCCGTCAAATTACCACTAATGAAACTATATCATTCATCCTCCTTCTTCTCCATCTCCCTTCTTCAACCCTTACCCTATCGGCAGCCATCGACCTCCACTCATCGAAATCCAAACCTTATGAACTAAATTGTTGAGTTTGAAATCGGAGAGACTAAAAGTGTATTTTTCCCCATAATAATTACCTAATATTGTTCAAATCAAATCTCAATTCTTTTCCACTTTCTTTGAATTATTATATTATAAAGAACTCTTTCATTTCTATTTCTATTTTGTTCATAACTTTTTTTCTAAACACCAACGTCAAAAAAAAAAAATGTACCATTTATAAGACAATAGTAGCATTCCATATAAATCCAGTACCCAATCTTTTGATCATATTTGTACTTCAATATCCAAATAATCAAATAAAAATAAAAGCTCAACAAATTGGAAAATAAATAAATAAAATTGGGATGGCGATGTGCAGGACGCGTGCAGGTACTTCCTAATAGAGAGCGGATTTGCACTGTTCGTAGCATTTTTAATAAACGTGGCCGTCGTTTCAGTTTCCGGCACCGTTTGTACCGTCGGAAAAATCTCCGAAACCACCGCCGATCAGTGCTCCGACATAACCCTCAACTCCGCCTCCTTCCTCCTCCAGGTCCGCCGCCGCTGACTATCCATTTATATCTTTCATGTTCAAAACGCTCCGTTTTGATTTTTTTTTTTTTTTTAAATTTTCAGAACGTTTTGGGAAAATCGAGCTCCACTATTTACGCCATTGCGTTGCTCGCCTCTGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGACAATTCATCATGCAGGTATTTTATTTCATAAACTCTATTAATTAAGTTATATAAATATATTTTTAATCTCAATTTCAATTTCAAATTTAGATTTAGATTTTTTTTTATTTATTTTAATTTTTAGGGGTTTTTGGACTTGAAAATGAAAACATGGGTGAGGAACCTTATGACGAGGTCCATTGCCATTACGCCCAGTCTCATTGTCGCCATTATCGGTGGACCTCAGGGAGCTGGACGTCTCATCATTATTGCTTCGGTTTGTATTTTCTTTATTTTGTTACTTTTTTAAAAGAAAATTACAATTTTTTTTTTGACAATACGTGGAGAGAGAGATTCGAACTACAGACCATGTGGTTATCAGTACAACTTTATGTCAATTGGGCTACGTTTTTGTTGGCAGAAAATTACAAATTTGGAGCAACTAGAAAGTGTCAAAAAGATCTCCACTTTCAATCTTTAAAGAAATATCCAATAGATCACTTTTAATTTTAAGTAAAATAAGCTATTGACTTAATCGACATTTTTTAAAATTTAATGGTTTAGTAGATACAAAATTGGAAGTTTAGGATCCTAGCAAACATAAAATTAAATTTTATATTTAGTAGATTTGTTGATCTTTAAAAATATTGAATGTTTTTTTTTTTACAATGTGTGGGGTGGAGGATTCGAACCACAGACCTCGTAGTCATCAGTACAACTTTATGTCGGTTGAGCTAAGCTCTTGTTGATTTATTAGATATTTTTAAAGTTCAAGAACTTATTATACATAAAATTAAAAGTTGAGAGACATATTAGATATTTTTTAAAATCTTGGAACTTAATCAGCTAACCCATTAACTCGCGGATACTCATGTCAAATTTACCCATTTATGTTCTCAAATATCTCACTCTACTTATCTTCAATATGTATGTACGTATATTTCACTTTTTAAACAAATTTAGTAATCACTCTACCTTATCCATTAATCTATGCTTATTCGCGATAAAATATATGATTTAAACTATTTTCATGAATTTCATTCTAAAATATATATATATATGTATATATATATGAATTTTAAAAATAAATTTAATGGGCTAATGCGTAATTTCGCAGATGATTCTATCCTTCGAGCTCCCATTCGCTCTAATTCCGCTACTCAAATTCAGCAGCAGCAGCACGAAGATGGGCCCCCACAAGAATTCCATATACGTAAGCAAGTTTCCCCCTTGTCATTACACTACCGATATTGGGCCGACCCATTAACAACTTTTACATCTATTTGGGCTTTTTTCAGATCATTGTAATCTCATGGATATTGGGCCTGGGAATCATCGGCATCAACATCTACTATCTGAGTACGGGCTTCGTGGGCTGGCTGATCGACAACAATTTACCAAAAGTTGCAAATGTATTCATTGGGATCGTTGTGTTTCCATTGATGGCCTTGTACATTCTTGCCGTTATTTACCTAACGTTTCGAAAGGACAGGGTTGTCACTTACATTGAGCCCGAAAAGTACGACCCTATGGCCCAAGCCCATTTGGAAGGTGGGCTGAATAATTCTTTTGGGCCTGGTGACGTCGCCCCGGTCCCTTTCAGAGAGGACTTGGCCCATATCCCACTTCCGGAGTAGTGGCGTTGTTGTATTTGTCCGGCCCAAATGGGCTGTTGCGAGTCTGGGCCTCGGTCCAACTCCGGATGATGGGCTTTATTGCCACTGTGGCCCACTAATGTGTCTGTATTTTGCTTCTGCTCGTCTTGGCTAGGGGCTAACCTGGAATTTTTGCTTGCGTGTCATGTGCCATGTATTCCCGGAATCTTCTGATAATGTATAATTAAAATAACAACAAAAATAAATTATTGATTTCATTTTCCTT

mRNA sequence

ATGGAGGACGGGATGAAAGCACACGTTGAGACGGCTAGATCCGACGAGATCGTGAGAGCAGAAACCAGATTTGGCGTCGTCGGAGCCGACAAGGACTATGTCGGAGAAGAAGAACTTTTGAGGTTGTCGGCGGCCGGCGTTAGCGGTGGCTGTGGCCGGCGACGGCTGACGGAAGAAGAAGGAAGAAGGCCGACGGAGAAGAAGAAGAATTGGAGGCCGAAGGAGAAGAAGAAAAAGGTGTTTTTATACACAACCACATTGAAGAGAGAGAGAGAAGAAATGGCAAGTCGGCAGAAGGAAGCTCAGATGAGTACGGCGGCGACACCGTCTTTTGGTGGTGGCAGCAACCGCATAGCGGCAGTGACGACCACTCCGGCCAACGCCGTCAAGCCACAAAATCACCATGAAGAACAAACCACCACGCATCAGAAACCTGGATGGAGAAAATTTCTTTCACACGTTGGCCCTGGCTTCCTCGTCTCATTGGCTTACCTTGATCCCGGCAACTTGGAAACCGATTTGCAGGCTGGAGCTAATCACGGATACGAGCTTTTGTGGGTGGTGCTTATCGGATTAATATTTGCGCTCATAATCCAATCTCTCGCTGCAAATCTCGGCGTAAGCACTGAAGTTTGCAAAGCCGAATATCCCAAATTTGTGAAGTACTTCCTTTGGTTGCTGGCTGAAGTTGCTGTAATAGCCGCCGATATTCCAGAAGTGATAGGAACGGCCTTTGCCCTGAATATACTATTCCATATTCCAGTTTGGGCGGGAGTTCTTCTCACCGGATTAAGCACCCTCTTGCTCCTTGGACTCCAGAGATATGGGGCGAGGAAGCTGGAATTGCTGATAGCAGTTTTGGTATTCGTAATGGCGGCGTGTTTCTTCGGGGAAATGAGTTACGTTAAGCCGCCGGCGACCGGCGTTTTGAAAGGAATGTTTGTGCCGAAACTCAACGGCCAAGGAGCCACCGCCGACGCCATTGCTCTCCTCGGCGCTCTCGTTATGCCGCACAATCTCTTTCTTCATTCGGCTCTCGTGCTCTCTAGGAAGGTGCCAAATTCCGTCCGCGGTATCAACGACGCGTGCAGGTACTTCCTAATAGAGAGCGGATTTGCACTGTTCGTAGCATTTTTAATAAACGTGGCCGTCGTTTCAGTTTCCGGCACCGTTTGTACCGTCGGAAAAATCTCCGAAACCACCGCCGATCAGTGCTCCGACATAACCCTCAACTCCGCCTCCTTCCTCCTCCAGAACGTTTTGGGAAAATCGAGCTCCACTATTTACGCCATTGCGTTGCTCGCCTCTGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGACAATTCATCATGCAGGGGTTTTTGGACTTGAAAATGAAAACATGGGTGAGGAACCTTATGACGAGGTCCATTGCCATTACGCCCAGTCTCATTGTCGCCATTATCGGTGGACCTCAGGGAGCTGGACGTCTCATCATTATTGCTTCGATCATTGTAATCTCATGGATATTGGGCCTGGGAATCATCGGCATCAACATCTACTATCTGAGTACGGGCTTCGTGGGCTGGCTGATCGACAACAATTTACCAAAAGTTGCAAATGTATTCATTGGGATCGTTGTGTTTCCATTGATGGCCTTGTACATTCTTGCCGTTATTTACCTAACGTTTCGAAAGGACAGGGTTGTCACTTACATTGAGCCCGAAAAGTACGACCCTATGGCCCAAGCCCATTTGGAAGGTGGGCTGAATAATTCTTTTGGGCCTGGTGACGTCGCCCCGGTCCCTTTCAGAGAGGACTTGGCCCATATCCCACTTCCGGAGTAG

Coding sequence (CDS)

ATGGAGGACGGGATGAAAGCACACGTTGAGACGGCTAGATCCGACGAGATCGTGAGAGCAGAAACCAGATTTGGCGTCGTCGGAGCCGACAAGGACTATGTCGGAGAAGAAGAACTTTTGAGGTTGTCGGCGGCCGGCGTTAGCGGTGGCTGTGGCCGGCGACGGCTGACGGAAGAAGAAGGAAGAAGGCCGACGGAGAAGAAGAAGAATTGGAGGCCGAAGGAGAAGAAGAAAAAGGTGTTTTTATACACAACCACATTGAAGAGAGAGAGAGAAGAAATGGCAAGTCGGCAGAAGGAAGCTCAGATGAGTACGGCGGCGACACCGTCTTTTGGTGGTGGCAGCAACCGCATAGCGGCAGTGACGACCACTCCGGCCAACGCCGTCAAGCCACAAAATCACCATGAAGAACAAACCACCACGCATCAGAAACCTGGATGGAGAAAATTTCTTTCACACGTTGGCCCTGGCTTCCTCGTCTCATTGGCTTACCTTGATCCCGGCAACTTGGAAACCGATTTGCAGGCTGGAGCTAATCACGGATACGAGCTTTTGTGGGTGGTGCTTATCGGATTAATATTTGCGCTCATAATCCAATCTCTCGCTGCAAATCTCGGCGTAAGCACTGAAGTTTGCAAAGCCGAATATCCCAAATTTGTGAAGTACTTCCTTTGGTTGCTGGCTGAAGTTGCTGTAATAGCCGCCGATATTCCAGAAGTGATAGGAACGGCCTTTGCCCTGAATATACTATTCCATATTCCAGTTTGGGCGGGAGTTCTTCTCACCGGATTAAGCACCCTCTTGCTCCTTGGACTCCAGAGATATGGGGCGAGGAAGCTGGAATTGCTGATAGCAGTTTTGGTATTCGTAATGGCGGCGTGTTTCTTCGGGGAAATGAGTTACGTTAAGCCGCCGGCGACCGGCGTTTTGAAAGGAATGTTTGTGCCGAAACTCAACGGCCAAGGAGCCACCGCCGACGCCATTGCTCTCCTCGGCGCTCTCGTTATGCCGCACAATCTCTTTCTTCATTCGGCTCTCGTGCTCTCTAGGAAGGTGCCAAATTCCGTCCGCGGTATCAACGACGCGTGCAGGTACTTCCTAATAGAGAGCGGATTTGCACTGTTCGTAGCATTTTTAATAAACGTGGCCGTCGTTTCAGTTTCCGGCACCGTTTGTACCGTCGGAAAAATCTCCGAAACCACCGCCGATCAGTGCTCCGACATAACCCTCAACTCCGCCTCCTTCCTCCTCCAGAACGTTTTGGGAAAATCGAGCTCCACTATTTACGCCATTGCGTTGCTCGCCTCTGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGACAATTCATCATGCAGGGGTTTTTGGACTTGAAAATGAAAACATGGGTGAGGAACCTTATGACGAGGTCCATTGCCATTACGCCCAGTCTCATTGTCGCCATTATCGGTGGACCTCAGGGAGCTGGACGTCTCATCATTATTGCTTCGATCATTGTAATCTCATGGATATTGGGCCTGGGAATCATCGGCATCAACATCTACTATCTGAGTACGGGCTTCGTGGGCTGGCTGATCGACAACAATTTACCAAAAGTTGCAAATGTATTCATTGGGATCGTTGTGTTTCCATTGATGGCCTTGTACATTCTTGCCGTTATTTACCTAACGTTTCGAAAGGACAGGGTTGTCACTTACATTGAGCCCGAAAAGTACGACCCTATGGCCCAAGCCCATTTGGAAGGTGGGCTGAATAATTCTTTTGGGCCTGGTGACGTCGCCCCGGTCCCTTTCAGAGAGGACTTGGCCCATATCCCACTTCCGGAGTAG

Protein sequence

MEDGMKAHVETARSDEIVRAETRFGVVGADKDYVGEEELLRLSAAGVSGGCGRRRLTEEEGRRPTEKKKNWRPKEKKKKVFLYTTTLKREREEMASRQKEAQMSTAATPSFGGGSNRIAAVTTTPANAVKPQNHHEEQTTTHQKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVSTEVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASIIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPKVANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGDVAPVPFREDLAHIPLPE
Homology
BLAST of Spg008711 vs. NCBI nr
Match: XP_023526032.1 (metal transporter Nramp5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 903.3 bits (2333), Expect = 1.2e-258
Identity = 485/557 (87.07%), Postives = 502/557 (90.13%), Query Frame = 0

Query: 95  ASRQKEAQMSTAATPSFGGGSNRIAAV-----TTTPANAVKPQNHHEEQTTTHQKPGWRK 154
           +S+QKEAQMST ATPSFGGGSNRIAAV     TT PA+AVKPQNH EE TTT QKPGWRK
Sbjct: 3   SSQQKEAQMSTVATPSFGGGSNRIAAVNTAETTTIPADAVKPQNHREEPTTTDQKPGWRK 62

Query: 155 FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST 214
           FLSHVGPGFLVSLAYLDPGNLETDLQAGANHG+ELLWVVL+GLIFALIIQSLAANLGVST
Sbjct: 63  FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLVGLIFALIIQSLAANLGVST 122

Query: 215 -----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 274
                EVCKAEYPK VKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL
Sbjct: 123 GKHLSEVCKAEYPKLVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 182

Query: 275 STLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 334
           STLLL+GLQRYG RKLELLIA+LVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT
Sbjct: 183 STLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 242

Query: 335 ADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAV 394
            DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALF++FLIN+AV
Sbjct: 243 GDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAV 302

Query: 395 VSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 454
           VSVSGTVCTVG +SE+TADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG
Sbjct: 303 VSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 362

Query: 455 TYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS------- 514
           TYAGQFIMQGFLDLKMKTW+RNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS       
Sbjct: 363 TYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFEL 422

Query: 515 -------------------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPK 574
                                    IIVISWILGL IIGINIYYLSTGFVGWLIDNNLPK
Sbjct: 423 PFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTGFVGWLIDNNLPK 482

Query: 575 VANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGD 610
           VANVFIGIVVFPLMA+YILAVIYLTFRKDRVVTYIEP+K DPMAQAHLE GL+NSFGPGD
Sbjct: 483 VANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGD 542

BLAST of Spg008711 vs. NCBI nr
Match: XP_022953164.1 (metal transporter Nramp5-like [Cucurbita moschata])

HSP 1 Score: 902.9 bits (2332), Expect = 1.5e-258
Identity = 486/557 (87.25%), Postives = 502/557 (90.13%), Query Frame = 0

Query: 95  ASRQKEAQMSTAATPSFGGGSNRIAAV-----TTTPANAVKPQNHHEEQTTTHQKPGWRK 154
           +S+QKEAQMST ATPSFGGGSNRIAAV     TT PA+AVK QNH EE TTT QKPGWRK
Sbjct: 3   SSQQKEAQMSTVATPSFGGGSNRIAAVNTAETTTIPADAVKTQNHREEPTTTDQKPGWRK 62

Query: 155 FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST 214
           FLSHVGPGFLVSLAYLDPGNLETDLQAGANHG+ELLWVVLIGLIFALIIQSLAANLGVST
Sbjct: 63  FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST 122

Query: 215 -----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 274
                EVCKAEYPKFVKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL
Sbjct: 123 GKHLSEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 182

Query: 275 STLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 334
           STLLL+GLQRYG RKLELLIA+LVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT
Sbjct: 183 STLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 242

Query: 335 ADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAV 394
            DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALF++FLIN+AV
Sbjct: 243 GDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAV 302

Query: 395 VSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 454
           VSVSGTVCTVG +SE+TADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG
Sbjct: 303 VSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 362

Query: 455 TYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS------- 514
           TYAGQFIMQGFLDLKMKTW+RNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS       
Sbjct: 363 TYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFEL 422

Query: 515 -------------------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPK 574
                                    IIVISWILGL IIGINIYYLSTGFVGWLIDNNLPK
Sbjct: 423 PFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTGFVGWLIDNNLPK 482

Query: 575 VANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGD 610
           VANVFIGIVVFPLMA+YILAVIYLTFRKDRVVTYIEP+K DPMAQAHLE GL+NSFGPGD
Sbjct: 483 VANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGD 542

BLAST of Spg008711 vs. NCBI nr
Match: XP_038886296.1 (metal transporter Nramp5-like [Benincasa hispida])

HSP 1 Score: 895.6 bits (2313), Expect = 2.4e-256
Identity = 484/558 (86.74%), Postives = 493/558 (88.35%), Query Frame = 0

Query: 94  MASRQKEAQMSTAATPSFGGGSNRIAAV-----TTTPANAVKPQNHHEEQTTTHQKPGWR 153
           M S+QKEAQMST ATPSFGGGSNRIAAV     T TP N VKPQNH EEQT  HQKPGWR
Sbjct: 1   MGSQQKEAQMSTVATPSFGGGSNRIAAVNTGVATATPTNTVKPQNHREEQTINHQKPGWR 60

Query: 154 KFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVS 213
           KFLSHVGPGFLVSLAYLDPGNLETDLQAGANHG+ELLWVVLIGLIFALIIQSLAANLGVS
Sbjct: 61  KFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVS 120

Query: 214 T-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTG 273
           T     EVCKAEYP FVKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTG
Sbjct: 121 TGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTG 180

Query: 274 LSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGA 333
           LSTLLLLGLQRYGARKLELLIA+LVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGA
Sbjct: 181 LSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGA 240

Query: 334 TADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVA 393
           TADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGIN+ACRYFLIESGFALF+AFLINVA
Sbjct: 241 TADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINEACRYFLIESGFALFIAFLINVA 300

Query: 394 VVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSIT 453
           VVSVSGTVCTVGK+SETTADQCSDITLNSASFLLQNVLGKSSSTIYAIAL ASGQSSSIT
Sbjct: 301 VVSVSGTVCTVGKVSETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSIT 360

Query: 454 GTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS------ 513
           GTYAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIAS      
Sbjct: 361 GTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFE 420

Query: 514 --------------------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLP 573
                                     IIVISWILGL IIGINIYYLST FVGWLI NNL 
Sbjct: 421 LPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLH 480

Query: 574 KVANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPG 610
           ++ANVFIGIVVFPLMA YILAVIYL FRKDRVVTYIEPEK DPMAQAHLE GLNNSFGP 
Sbjct: 481 RIANVFIGIVVFPLMAAYILAVIYLMFRKDRVVTYIEPEKDDPMAQAHLESGLNNSFGPR 540

BLAST of Spg008711 vs. NCBI nr
Match: XP_022991146.1 (metal transporter Nramp5-like [Cucurbita maxima])

HSP 1 Score: 891.7 bits (2303), Expect = 3.5e-255
Identity = 480/557 (86.18%), Postives = 498/557 (89.41%), Query Frame = 0

Query: 95  ASRQKEAQMSTAATPSFGGGSNRIAAV-----TTTPANAVKPQNHHEEQTTTHQKPGWRK 154
           +S+QKE+QMS  ATPSFG GSNRIAAV     TT PA+AVKPQNH E  TTT QKPGWRK
Sbjct: 3   SSQQKESQMSIVATPSFGSGSNRIAAVNTAKITTIPADAVKPQNHREVPTTTDQKPGWRK 62

Query: 155 FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST 214
           FLSHVGPGFLVSLAYLDPGNLETDLQAGANHG+ELLWVVLIGLIFALIIQSLAANLGVST
Sbjct: 63  FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST 122

Query: 215 -----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 274
                EVC+AEYPK VKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL
Sbjct: 123 GKHLSEVCRAEYPKLVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 182

Query: 275 STLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 334
           STLLL+GLQRYG RKLELLIA+LVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT
Sbjct: 183 STLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 242

Query: 335 ADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAV 394
            DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALF++FLIN+AV
Sbjct: 243 GDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAV 302

Query: 395 VSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 454
           VSVSGTVCTVG +SE+TADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG
Sbjct: 303 VSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 362

Query: 455 TYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS------- 514
           TYAGQFIMQGFLDLKMKTW+RNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS       
Sbjct: 363 TYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFEL 422

Query: 515 -------------------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPK 574
                                    IIVISWILGL IIGINIYYLST FVGWLIDNNLPK
Sbjct: 423 PFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTAFVGWLIDNNLPK 482

Query: 575 VANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGD 610
           VANVFIGIVVFPLMA+YILAVIYLTFRKDRVVTYIEP+K DPMAQAHLE GL+NSFGPGD
Sbjct: 483 VANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGD 542

BLAST of Spg008711 vs. NCBI nr
Match: XP_011658448.2 (metal transporter Nramp5 [Cucumis sativus] >KGN47700.1 hypothetical protein Csa_018232 [Cucumis sativus])

HSP 1 Score: 874.4 bits (2258), Expect = 5.7e-250
Identity = 472/548 (86.13%), Postives = 486/548 (88.69%), Query Frame = 0

Query: 100 EAQMSTAATPSFGGGSNRIAAVT-TTPANAVKPQNHHEEQTTTHQKPGWRKFLSHVGPGF 159
           EAQMSTAATPSFGGGSNRIAAV  TTP NA+KPQNH E + TTHQKPGWRKFLS+VGPGF
Sbjct: 2   EAQMSTAATPSFGGGSNRIAAVNITTPTNALKPQNHQEGKNTTHQKPGWRKFLSYVGPGF 61

Query: 160 LVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST-----EVCK 219
           LVSLAYLDPGNLETDLQAGANHG+ELLWVVLIGLIFALIIQSLAANLGVST     EVCK
Sbjct: 62  LVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTGKHLSEVCK 121

Query: 220 AEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ 279
           AEYPKFVKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ
Sbjct: 122 AEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ 181

Query: 280 RYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGA 339
           RYGARKLELLIA+LVFVMAACFFGEMSYVKPPA+GVLKGMFVPKL G+GATADAIALLGA
Sbjct: 182 RYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLKGEGATADAIALLGA 241

Query: 340 LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAVVSVSGTVCT 399
           LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESG ALF+AFLINVAVVSVSGTVCT
Sbjct: 242 LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAFLINVAVVSVSGTVCT 301

Query: 400 VGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGTYAGQFIMQ 459
           VG +++TTADQCSDITLNSASFLLQNVLGKSSSTIYAIAL ASGQSSSITGTYAGQFIMQ
Sbjct: 302 VGNVTQTTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSITGTYAGQFIMQ 361

Query: 460 GFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS---------------- 519
           GFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIAS                
Sbjct: 362 GFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLK 421

Query: 520 ----------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPKVANVFIGIV 579
                           IIVISWILGL IIGINIYYLST FV WLI NNLPKVANVFIGI 
Sbjct: 422 FSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLIHNNLPKVANVFIGIA 481

Query: 580 VFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGDVAPVPFRED 610
           VFPLMA YI AVIYL FRKDRVVTYIEPEK D +AQAHLE GLNNSFGP DV P+PFRED
Sbjct: 482 VFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAVAQAHLESGLNNSFGPRDVTPLPFRED 541

BLAST of Spg008711 vs. ExPASy Swiss-Prot
Match: Q8H4H5 (Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 9.7e-184
Identity = 341/503 (67.79%), Postives = 399/503 (79.32%), Query Frame = 0

Query: 143 QKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLA 202
           ++P W++FL+HVGPGF+VSLAYLDPGNLETDLQAGANH YELLWV+LIGLIFALIIQSLA
Sbjct: 37  KEPAWKRFLAHVGPGFMVSLAYLDPGNLETDLQAGANHRYELLWVILIGLIFALIIQSLA 96

Query: 203 ANLGVST-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWA 262
           ANLGV T     E+CK+EYPKFVK FLWLLAE+AVIAADIPEVIGTAFA NILFHIPVW 
Sbjct: 97  ANLGVVTGRHLAEICKSEYPKFVKIFLWLLAELAVIAADIPEVIGTAFAFNILFHIPVWV 156

Query: 263 GVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPK 322
           GVL+TG STLLLLGLQ+YG RKLE LI++LVFVMAACFFGE+S VKPPA  V+KG+F+P+
Sbjct: 157 GVLITGTSTLLLLGLQKYGVRKLEFLISMLVFVMAACFFGELSIVKPPAKEVMKGLFIPR 216

Query: 323 LNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVA 382
           LNG GATADAIALLGALVMPHNLFLHSALVLSRK P SVRGI D CR+FL ESGFALFVA
Sbjct: 217 LNGDGATADAIALLGALVMPHNLFLHSALVLSRKTPASVRGIKDGCRFFLYESGFALFVA 276

Query: 383 FLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASG 442
            LIN+AVVSVSGT C+   +S+  AD+C++++L+++SFLL+NVLGKSS+ +Y +ALLASG
Sbjct: 277 LLINIAVVSVSGTACSSANLSQEDADKCANLSLDTSSFLLKNVLGKSSAIVYGVALLASG 336

Query: 443 QSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS 502
           QSS+ITGTYAGQ+IMQGFLD++M+ W+RNLMTR+IAI PSLIV+IIGG +GAGRLIIIAS
Sbjct: 337 QSSTITGTYAGQYIMQGFLDIRMRKWLRNLMTRTIAIAPSLIVSIIGGSRGAGRLIIIAS 396

Query: 503 --------------------------------IIVISWILGLGIIGINIYYLSTGFVGWL 562
                                           IIV SW LGL IIGIN+Y+LST FVGWL
Sbjct: 397 MILSFELPFALIPLLKFSSSKSKMGPHKNSIYIIVFSWFLGLLIIGINMYFLSTSFVGWL 456

Query: 563 IDNNLPKVANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLN 609
           I N+LPK ANV +G  VFP M +YI+AV+YLT RKD VVT++       +  A  E    
Sbjct: 457 IHNDLPKYANVLVGAAVFPFMLVYIVAVVYLTIRKDSVVTFVADSSLAAVVDA--EKADA 516

BLAST of Spg008711 vs. ExPASy Swiss-Prot
Match: Q0D7E4 (Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.8e-167
Identity = 308/458 (67.25%), Postives = 365/458 (79.69%), Query Frame = 0

Query: 143 QKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLA 202
           ++P W++FLSH+GPGF+V LAYLDPGN+ETDLQAGANH YELLWV+LIGLIFALIIQSL+
Sbjct: 28  KEPAWKRFLSHIGPGFMVCLAYLDPGNMETDLQAGANHKYELLWVILIGLIFALIIQSLS 87

Query: 203 ANLGVST-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWA 262
           ANLGV T     E+CK EYP +VK  LWLLAE+AVIA+DIPEVIGT FA N+LFHIPVW 
Sbjct: 88  ANLGVVTGRHLAELCKTEYPVWVKTCLWLLAELAVIASDIPEVIGTGFAFNLLFHIPVWT 147

Query: 263 GVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPK 322
           GVL+ G STLLLLGLQRYG RKLE+++A+LVFVMA CFF EMS VKPP   VL+G+F+P+
Sbjct: 148 GVLIAGSSTLLLLGLQRYGVRKLEVVVALLVFVMAGCFFVEMSIVKPPVNEVLQGLFIPR 207

Query: 323 LNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVA 382
           L+G GAT D+IALLGALVMPHNLFLHSALVLSR  P S +G+ D CR+FL ESG ALFVA
Sbjct: 208 LSGPGATGDSIALLGALVMPHNLFLHSALVLSRNTPASAKGMKDVCRFFLFESGIALFVA 267

Query: 383 FLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASG 442
            L+N+A++SVSGTVC    +S   A +CSD+TL+S+SFLL+NVLGKSS+T+Y +ALLASG
Sbjct: 268 LLVNIAIISVSGTVCNATNLSPEDAVKCSDLTLDSSSFLLRNVLGKSSATVYGVALLASG 327

Query: 443 QSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS 502
           QSS+ITGTYAGQ++MQGFLD+KMK W+RNLMTRSIAI PSLIV+IIGG  GAGRLI+IAS
Sbjct: 328 QSSTITGTYAGQYVMQGFLDIKMKQWLRNLMTRSIAIVPSLIVSIIGGSSGAGRLIVIAS 387

Query: 503 IIV--------------------------------ISWILGLGIIGINIYYLSTGFVGWL 562
           +I+                                 SW+LG  IIGINIY+LST  VGW+
Sbjct: 388 MILSFELPFALIPLLKFSSSSNKMGENKNSIYIVGFSWVLGFVIIGINIYFLSTKLVGWI 447

Query: 563 IDNNLPKVANVFIGIVVFPLMALYILAVIYLTFRKDRV 564
           + N LP  ANV IGIV+FPLM LY++AVIYLTFRKD V
Sbjct: 448 LHNALPTFANVLIGIVLFPLMLLYVVAVIYLTFRKDTV 485

BLAST of Spg008711 vs. ExPASy Swiss-Prot
Match: Q6ZG85 (Metal transporter NRAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAT1 PE=2 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.8e-151
Identity = 291/524 (55.53%), Postives = 374/524 (71.37%), Query Frame = 0

Query: 128 AVKPQNHHEEQTTTHQK-------PGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANH 187
           +V   + ++E+T   +K       P WRKFL+HVGPG LV++ +LDP NLETD+QAGA+ 
Sbjct: 22  SVSETDEYKEKTIDSEKDGQFRVQPRWRKFLAHVGPGALVAIGFLDPSNLETDMQAGADF 81

Query: 188 GYELLWVVLIGLIFALIIQSLAANLGVST-----EVCKAEYPKFVKYFLWLLAEVAVIAA 247
            YELLWV+L+G++FAL+IQ+LAANLGV T     E+C+ EYP +V  FLW++AE+AVI+ 
Sbjct: 82  KYELLWVILVGMVFALLIQTLAANLGVKTGRHLAELCREEYPHYVNIFLWIIAELAVISD 141

Query: 248 DIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACF 307
           DIPEV+GTAFA NIL  IPVWAGV+LT  STLLLLG+QR+GARKLE +IA  +F MAACF
Sbjct: 142 DIPEVLGTAFAFNILLKIPVWAGVILTVFSTLLLLGVQRFGARKLEFIIAAFMFTMAACF 201

Query: 308 FGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNS 367
           FGE+SY++P A  V+KGMFVP L G+GA A+AIAL GA++ P+NLFLHSALVLSRK P S
Sbjct: 202 FGELSYLRPSAGEVVKGMFVPSLQGKGAAANAIALFGAIITPYNLFLHSALVLSRKTPRS 261

Query: 368 VRGINDACRYFLIESGFALFVAFLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASF 427
            + I  ACRYFLIE   A  VAFLINV+VV V+G++C    +S   A+ C D+TL S   
Sbjct: 262 DKSIRAACRYFLIECSLAFIVAFLINVSVVVVAGSICNANNLSPADANTCGDLTLQSTPL 321

Query: 428 LLQNVLGKSSSTIYAIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAIT 487
           LL+NVLG+SSS +YA+ALLASGQS++I+ T+AGQ IMQGFLD+KMK WVRNL+TR IAI 
Sbjct: 322 LLRNVLGRSSSVVYAVALLASGQSTTISCTFAGQVIMQGFLDMKMKNWVRNLITRVIAIA 381

Query: 488 PSLIVAIIGGPQGAGRLIIIASII--------------------------------VISW 547
           PSLIV+I+ GP GAG+LII++S+I                                VI+W
Sbjct: 382 PSLIVSIVSGPSGAGKLIILSSMILSFELPFALIPLLKFCNSSKKVGPLKESIYTVVIAW 441

Query: 548 ILGLGIIGINIYYLSTGFVGWLIDNNLPKVANVFIGIVVFPLMALYILAVIYLTFRKDRV 604
           IL   +I +N Y+L   +V WL+ NNLPK AN  I +VVF LMA Y++AV+YLTFRKD V
Sbjct: 442 ILSFALIVVNTYFLVWTYVDWLVHNNLPKYANGLISVVVFALMAAYLVAVVYLTFRKDTV 501

BLAST of Spg008711 vs. ExPASy Swiss-Prot
Match: Q9SAH8 (Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 2.0e-141
Identity = 273/477 (57.23%), Postives = 345/477 (72.33%), Query Frame = 0

Query: 126 ANAVKPQNHHEEQTTTHQKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELL 185
           +N+   +N    Q    +K  W+ F +++GPGFLVS+AY+DPGN ETDLQAGA++ YELL
Sbjct: 22  SNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSIAYIDPGNFETDLQAGAHYKYELL 81

Query: 186 WVVLIGLIFALIIQSLAANLGVST-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEV 245
           W++L+    AL+IQSLAANLGV T     E C+AEY K   + LW++AE+AV+A DIPEV
Sbjct: 82  WIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYSKVPNFMLWVVAEIAVVACDIPEV 141

Query: 246 IGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMS 305
           IGTAFALN+LF IPVW GVLLTGLSTL+LL LQ+YG RKLE LIA LVF +A CFF E+ 
Sbjct: 142 IGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGVRKLEFLIAFLVFTIAICFFVELH 201

Query: 306 YVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGIN 365
           Y KP    VL G+FVP+L G GAT  AI+LLGA+VMPHNLFLHSALVLSRK+P S  GI 
Sbjct: 202 YSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSASGIK 261

Query: 366 DACRYFLIESGFALFVAFLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNV 425
           +ACR++LIESG AL VAFLINV+V+SVSG VC    +S      C D+ LN ASFLL+NV
Sbjct: 262 EACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNLSPEDRANCEDLDLNKASFLLRNV 321

Query: 426 LGKSSSTIYAIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIV 485
           +GK SS ++AIALLASGQSS+ITGTYAGQ++MQGFLDL+++ W+RNL+TR +AI PSLIV
Sbjct: 322 VGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNLLTRCLAIIPSLIV 381

Query: 486 AIIGGPQGAGRLIIIASIIV--------------------------------ISWILGLG 545
           A+IGG  GAG+LIIIAS+I+                                ++W++G  
Sbjct: 382 ALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSCKTKMGSHVNPMAITALTWVIGGL 441

Query: 546 IIGINIYYLSTGFVGWLIDNNLPKVANVFIGIVVFPLMALYILAVIYLTFRKDRVVT 566
           I+GINIYYL + F+  LI +++  +  VF GI+ F  +ALY+ A+ YL FRK+RV T
Sbjct: 442 IMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAGIALYLAAIAYLVFRKNRVAT 498

BLAST of Spg008711 vs. ExPASy Swiss-Prot
Match: Q653V6 (Metal transporter Nramp3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP3 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.7e-140
Identity = 279/510 (54.71%), Postives = 356/510 (69.80%), Query Frame = 0

Query: 137 EQTTTHQKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFAL 196
           +Q    +K  W+   S++GPGFLVS+AY+DPGN ETDLQAGA + YELLW++LI    AL
Sbjct: 37  DQIVIPEKNSWKNLFSYIGPGFLVSIAYIDPGNFETDLQAGAQYKYELLWIILIASCAAL 96

Query: 197 IIQSLAANLGVST-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILF 256
           IIQSLAA LGV T     E C+AEYPK   + LW+LAE+AV+A DIPEVIGTAFALN+LF
Sbjct: 97  IIQSLAARLGVVTGKHLAEHCRAEYPKATNFILWILAELAVVACDIPEVIGTAFALNMLF 156

Query: 257 HIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLK 316
            IPVW GVL+TGLSTL+LL LQ+YG RKLE LIA+LV ++A CF  E+ Y KP ++ V++
Sbjct: 157 KIPVWCGVLITGLSTLMLLLLQQYGVRKLEFLIAILVSLIATCFLVELGYSKPNSSEVVR 216

Query: 317 GMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESG 376
           G+FVP+L G GAT  AI+LLGA+VMPHNLFLHSALVLSRKVP SV GI +ACR+++IES 
Sbjct: 217 GLFVPELKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKVPRSVHGIKEACRFYMIESA 276

Query: 377 FALFVAFLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAI 436
           FAL +AFLIN++++SVSG VC    +S      CSD+ LN ASFLL+NVLG  SS ++A+
Sbjct: 277 FALTIAFLINISIISVSGAVCGSDNLSPEDQMNCSDLDLNKASFLLKNVLGNWSSKLFAV 336

Query: 437 ALLASGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGR 496
           ALLASGQSS+ITGTYAGQ++MQGFLDL+M  W+RNL+TRS+AI PSLIV+IIGG   AG+
Sbjct: 337 ALLASGQSSTITGTYAGQYVMQGFLDLRMTPWIRNLLTRSLAILPSLIVSIIGGSSAAGQ 396

Query: 497 LIIIASII--------------------------------VISWILGLGIIGINIYYLST 556
           LIIIAS+I                                VI+W +G  I+ IN Y+L T
Sbjct: 397 LIIIASMILSFELPFALVPLLKFTSSRTKMGQHTNSKAISVITWGIGSFIVVINTYFLIT 456

Query: 557 GFVGWLIDNNLPKVANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAH 610
            FV  L+ N L  V+ VF GI  F  M +Y+ A++YL FRK+R  T       +  +   
Sbjct: 457 SFVKLLLHNGLSTVSQVFSGIFGFLGMLIYMAAILYLVFRKNRKATL---PLLEGDSTVR 516

BLAST of Spg008711 vs. ExPASy TrEMBL
Match: A0A6J1GNU9 (metal transporter Nramp5-like OS=Cucurbita moschata OX=3662 GN=LOC111455783 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 7.3e-259
Identity = 486/557 (87.25%), Postives = 502/557 (90.13%), Query Frame = 0

Query: 95  ASRQKEAQMSTAATPSFGGGSNRIAAV-----TTTPANAVKPQNHHEEQTTTHQKPGWRK 154
           +S+QKEAQMST ATPSFGGGSNRIAAV     TT PA+AVK QNH EE TTT QKPGWRK
Sbjct: 3   SSQQKEAQMSTVATPSFGGGSNRIAAVNTAETTTIPADAVKTQNHREEPTTTDQKPGWRK 62

Query: 155 FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST 214
           FLSHVGPGFLVSLAYLDPGNLETDLQAGANHG+ELLWVVLIGLIFALIIQSLAANLGVST
Sbjct: 63  FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST 122

Query: 215 -----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 274
                EVCKAEYPKFVKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL
Sbjct: 123 GKHLSEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 182

Query: 275 STLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 334
           STLLL+GLQRYG RKLELLIA+LVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT
Sbjct: 183 STLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 242

Query: 335 ADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAV 394
            DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALF++FLIN+AV
Sbjct: 243 GDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAV 302

Query: 395 VSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 454
           VSVSGTVCTVG +SE+TADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG
Sbjct: 303 VSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 362

Query: 455 TYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS------- 514
           TYAGQFIMQGFLDLKMKTW+RNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS       
Sbjct: 363 TYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFEL 422

Query: 515 -------------------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPK 574
                                    IIVISWILGL IIGINIYYLSTGFVGWLIDNNLPK
Sbjct: 423 PFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTGFVGWLIDNNLPK 482

Query: 575 VANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGD 610
           VANVFIGIVVFPLMA+YILAVIYLTFRKDRVVTYIEP+K DPMAQAHLE GL+NSFGPGD
Sbjct: 483 VANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGD 542

BLAST of Spg008711 vs. ExPASy TrEMBL
Match: A0A6J1JPX4 (metal transporter Nramp5-like OS=Cucurbita maxima OX=3661 GN=LOC111487830 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.7e-255
Identity = 480/557 (86.18%), Postives = 498/557 (89.41%), Query Frame = 0

Query: 95  ASRQKEAQMSTAATPSFGGGSNRIAAV-----TTTPANAVKPQNHHEEQTTTHQKPGWRK 154
           +S+QKE+QMS  ATPSFG GSNRIAAV     TT PA+AVKPQNH E  TTT QKPGWRK
Sbjct: 3   SSQQKESQMSIVATPSFGSGSNRIAAVNTAKITTIPADAVKPQNHREVPTTTDQKPGWRK 62

Query: 155 FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST 214
           FLSHVGPGFLVSLAYLDPGNLETDLQAGANHG+ELLWVVLIGLIFALIIQSLAANLGVST
Sbjct: 63  FLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST 122

Query: 215 -----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 274
                EVC+AEYPK VKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL
Sbjct: 123 GKHLSEVCRAEYPKLVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGL 182

Query: 275 STLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 334
           STLLL+GLQRYG RKLELLIA+LVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT
Sbjct: 183 STLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 242

Query: 335 ADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAV 394
            DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALF++FLIN+AV
Sbjct: 243 GDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAV 302

Query: 395 VSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 454
           VSVSGTVCTVG +SE+TADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG
Sbjct: 303 VSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITG 362

Query: 455 TYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS------- 514
           TYAGQFIMQGFLDLKMKTW+RNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS       
Sbjct: 363 TYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFEL 422

Query: 515 -------------------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPK 574
                                    IIVISWILGL IIGINIYYLST FVGWLIDNNLPK
Sbjct: 423 PFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTAFVGWLIDNNLPK 482

Query: 575 VANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGD 610
           VANVFIGIVVFPLMA+YILAVIYLTFRKDRVVTYIEP+K DPMAQAHLE GL+NSFGPGD
Sbjct: 483 VANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGD 542

BLAST of Spg008711 vs. ExPASy TrEMBL
Match: A0A0A0KDB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G382880 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.8e-250
Identity = 472/548 (86.13%), Postives = 486/548 (88.69%), Query Frame = 0

Query: 100 EAQMSTAATPSFGGGSNRIAAVT-TTPANAVKPQNHHEEQTTTHQKPGWRKFLSHVGPGF 159
           EAQMSTAATPSFGGGSNRIAAV  TTP NA+KPQNH E + TTHQKPGWRKFLS+VGPGF
Sbjct: 2   EAQMSTAATPSFGGGSNRIAAVNITTPTNALKPQNHQEGKNTTHQKPGWRKFLSYVGPGF 61

Query: 160 LVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST-----EVCK 219
           LVSLAYLDPGNLETDLQAGANHG+ELLWVVLIGLIFALIIQSLAANLGVST     EVCK
Sbjct: 62  LVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTGKHLSEVCK 121

Query: 220 AEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ 279
           AEYPKFVKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ
Sbjct: 122 AEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ 181

Query: 280 RYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGA 339
           RYGARKLELLIA+LVFVMAACFFGEMSYVKPPA+GVLKGMFVPKL G+GATADAIALLGA
Sbjct: 182 RYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLKGEGATADAIALLGA 241

Query: 340 LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAVVSVSGTVCT 399
           LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESG ALF+AFLINVAVVSVSGTVCT
Sbjct: 242 LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAFLINVAVVSVSGTVCT 301

Query: 400 VGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGTYAGQFIMQ 459
           VG +++TTADQCSDITLNSASFLLQNVLGKSSSTIYAIAL ASGQSSSITGTYAGQFIMQ
Sbjct: 302 VGNVTQTTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSITGTYAGQFIMQ 361

Query: 460 GFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS---------------- 519
           GFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIAS                
Sbjct: 362 GFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLK 421

Query: 520 ----------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPKVANVFIGIV 579
                           IIVISWILGL IIGINIYYLST FV WLI NNLPKVANVFIGI 
Sbjct: 422 FSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLIHNNLPKVANVFIGIA 481

Query: 580 VFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGDVAPVPFRED 610
           VFPLMA YI AVIYL FRKDRVVTYIEPEK D +AQAHLE GLNNSFGP DV P+PFRED
Sbjct: 482 VFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAVAQAHLESGLNNSFGPRDVTPLPFRED 541

BLAST of Spg008711 vs. ExPASy TrEMBL
Match: A0A1S3CA24 (metal transporter Nramp5-like OS=Cucumis melo OX=3656 GN=LOC103498358 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 7.8e-245
Identity = 466/548 (85.04%), Postives = 477/548 (87.04%), Query Frame = 0

Query: 100 EAQMSTAATPSFGGGSNRIAAVT-TTPANAVKPQNHHEEQTTTHQKPGWRKFLSHVGPGF 159
           E QMST  TPS GGGSNRIAAV  TTP NAV  QNH EE+ TTHQKPGWRKF+SHVGPGF
Sbjct: 2   EVQMSTTVTPSLGGGSNRIAAVNITTPINAVNLQNHQEEKNTTHQKPGWRKFVSHVGPGF 61

Query: 160 LVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST-----EVCK 219
           LVSLAYLDPGNLETDLQAGANHG+ELLWVVLIGLIFALIIQSLAANLGVST     EVCK
Sbjct: 62  LVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTGKHLSEVCK 121

Query: 220 AEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ 279
           AEYP FVKY LWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ
Sbjct: 122 AEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQ 181

Query: 280 RYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGA 339
           RYGARKLELLIA+LVFVMAACFFGEMSYVKPPA+ VLKGMFVPKL G+GATADAIALLGA
Sbjct: 182 RYGARKLELLIAILVFVMAACFFGEMSYVKPPASSVLKGMFVPKLKGEGATADAIALLGA 241

Query: 340 LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAVVSVSGTVCT 399
           LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESG ALF+AFLINVAVVSVSGTVCT
Sbjct: 242 LVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAFLINVAVVSVSGTVCT 301

Query: 400 VGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGTYAGQFIMQ 459
           VG +++TTADQCSDITLNSASFLLQNVLGKSSSTIYAIAL ASGQSSSITGTYAGQFIMQ
Sbjct: 302 VGNVAQTTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSITGTYAGQFIMQ 361

Query: 460 GFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS---------------- 519
           GFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIAS                
Sbjct: 362 GFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLK 421

Query: 520 ----------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPKVANVFIGIV 579
                           IIVISWILGL IIGINIYYLST FV WLI NNL KVANVFIGI 
Sbjct: 422 FSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLIHNNLHKVANVFIGIA 481

Query: 580 VFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGDVAPVPFRED 610
           VFPLMA YI AVIYL FRKDRVVTYIEPEK D MAQAHLE GLNNSFGP DV PVPFRED
Sbjct: 482 VFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAMAQAHLETGLNNSFGPRDVTPVPFRED 541

BLAST of Spg008711 vs. ExPASy TrEMBL
Match: A0A6J1CJ54 (metal transporter Nramp5-like OS=Momordica charantia OX=3673 GN=LOC111011923 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 2.1e-242
Identity = 465/556 (83.63%), Postives = 482/556 (86.69%), Query Frame = 0

Query: 94  MASRQKEAQM--STAATPSFGGGSNRIAAVT-TTPANAVKPQNHHEEQTTTHQKPGWRKF 153
           M ++QKEAQM  +TAA PSFG GSNRIAA T T PA A K            +KPGWRKF
Sbjct: 3   MGTQQKEAQMITTTAAAPSFGSGSNRIAAETMTPPATAAK----------KIEKPGWRKF 62

Query: 154 LSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST- 213
           LSHVGPGFLVSLAYLDPGNLETDLQAGA+HGYELLWVVLIGLIFALIIQSLAANLGVST 
Sbjct: 63  LSHVGPGFLVSLAYLDPGNLETDLQAGASHGYELLWVVLIGLIFALIIQSLAANLGVSTG 122

Query: 214 ----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLS 273
               EVCKAEYPK VKY LWLLAEVAVIAADIPEV+GTAFALNILFHIPVWAGVLLTGLS
Sbjct: 123 KHLSEVCKAEYPKIVKYCLWLLAEVAVIAADIPEVVGTAFALNILFHIPVWAGVLLTGLS 182

Query: 274 TLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATA 333
           TLLLLGLQRYGARKLELLIA+LVFVMAACFFGEMSYVKPPA+GVLKGMFVPKLNGQGAT 
Sbjct: 183 TLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLNGQGATG 242

Query: 334 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAVV 393
           DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAVV
Sbjct: 243 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLINVAVV 302

Query: 394 SVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGT 453
           SVSGTVCTVG +S  TADQC DITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGT
Sbjct: 303 SVSGTVCTVGNVSPATADQCGDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGT 362

Query: 454 YAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIAS-------- 513
           YAGQFIMQGFLDLKMKTW RNLMTRSIAI PSL+V+IIGG QGAGRLIIIAS        
Sbjct: 363 YAGQFIMQGFLDLKMKTWARNLMTRSIAIAPSLVVSIIGGSQGAGRLIIIASMILSFELP 422

Query: 514 ------------------------IIVISWILGLGIIGINIYYLSTGFVGWLIDNNLPKV 573
                                   IIVISWILGL IIGINIYYLST FVGWLI ++LPKV
Sbjct: 423 FALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHSDLPKV 482

Query: 574 ANVFIGIVVFPLMALYILAVIYLTFRKDRVVTYIEPEKYDPMAQAHLEGGLNNSFGPGDV 610
           ANV IGIVVFPLMA+YILAVIYLTFRKDRVVTYIEPEK+DPMAQAH+E GL+NSFGPGDV
Sbjct: 483 ANVLIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPEKHDPMAQAHMESGLSNSFGPGDV 542

BLAST of Spg008711 vs. TAIR 10
Match: AT1G80830.1 (natural resistance-associated macrophage protein 1 )

HSP 1 Score: 504.2 bits (1297), Expect = 1.5e-142
Identity = 273/477 (57.23%), Postives = 345/477 (72.33%), Query Frame = 0

Query: 126 ANAVKPQNHHEEQTTTHQKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELL 185
           +N+   +N    Q    +K  W+ F +++GPGFLVS+AY+DPGN ETDLQAGA++ YELL
Sbjct: 22  SNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSIAYIDPGNFETDLQAGAHYKYELL 81

Query: 186 WVVLIGLIFALIIQSLAANLGVST-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEV 245
           W++L+    AL+IQSLAANLGV T     E C+AEY K   + LW++AE+AV+A DIPEV
Sbjct: 82  WIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYSKVPNFMLWVVAEIAVVACDIPEV 141

Query: 246 IGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMS 305
           IGTAFALN+LF IPVW GVLLTGLSTL+LL LQ+YG RKLE LIA LVF +A CFF E+ 
Sbjct: 142 IGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGVRKLEFLIAFLVFTIAICFFVELH 201

Query: 306 YVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGIN 365
           Y KP    VL G+FVP+L G GAT  AI+LLGA+VMPHNLFLHSALVLSRK+P S  GI 
Sbjct: 202 YSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSASGIK 261

Query: 366 DACRYFLIESGFALFVAFLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNV 425
           +ACR++LIESG AL VAFLINV+V+SVSG VC    +S      C D+ LN ASFLL+NV
Sbjct: 262 EACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNLSPEDRANCEDLDLNKASFLLRNV 321

Query: 426 LGKSSSTIYAIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIV 485
           +GK SS ++AIALLASGQSS+ITGTYAGQ++MQGFLDL+++ W+RNL+TR +AI PSLIV
Sbjct: 322 VGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNLLTRCLAIIPSLIV 381

Query: 486 AIIGGPQGAGRLIIIASIIV--------------------------------ISWILGLG 545
           A+IGG  GAG+LIIIAS+I+                                ++W++G  
Sbjct: 382 ALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSCKTKMGSHVNPMAITALTWVIGGL 441

Query: 546 IIGINIYYLSTGFVGWLIDNNLPKVANVFIGIVVFPLMALYILAVIYLTFRKDRVVT 566
           I+GINIYYL + F+  LI +++  +  VF GI+ F  +ALY+ A+ YL FRK+RV T
Sbjct: 442 IMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAGIALYLAAIAYLVFRKNRVAT 498

BLAST of Spg008711 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 499.6 bits (1285), Expect = 3.6e-141
Identity = 275/486 (56.58%), Postives = 348/486 (71.60%), Query Frame = 0

Query: 114 GSNRIAAVTTTPANAVKPQNHHEEQTTTHQKPGWRKFLSHVGPGFLVSLAYLDPGNLETD 173
           GSNR      + +N+   +N    Q    +K  W+ F S++GPGFLVS+AY+DPGN ETD
Sbjct: 8   GSNR------SISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETD 67

Query: 174 LQAGANHGYELLWVVLIGLIFALIIQSLAANLGVST-----EVCKAEYPKFVKYFLWLLA 233
           LQ+GA + YELLW++L+    AL+IQSLAANLGV T     E C+AEY K   + LW++A
Sbjct: 68  LQSGAQYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVA 127

Query: 234 EVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAVLV 293
           E+AV+A DIPEVIGTAFALN+LF+IPVW GVLLTGLSTL+LL LQ+YG RKLE LIA LV
Sbjct: 128 EIAVVACDIPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLV 187

Query: 294 FVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVL 353
           F +A CFF E+ Y KP    VL G+FVP+L G GAT  AI+LLGA+VMPHNLFLHSALVL
Sbjct: 188 FTIALCFFVELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVL 247

Query: 354 SRKVPNSVRGINDACRYFLIESGFALFVAFLINVAVVSVSGTVCTVGKISETTADQCSDI 413
           SRK+P SV GI +ACRY+LIESG AL VAFLINV+V+SVSG VC    +S      C D+
Sbjct: 248 SRKIPRSVTGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDL 307

Query: 414 TLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLM 473
            LN ASFLL+NV+GK SS ++AIALLASGQSS+ITGTYAGQ++MQGFLDL+++ W+RN +
Sbjct: 308 DLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFL 367

Query: 474 TRSIAITPSLIVAIIGGPQGAGRLIIIASIIV---------------------------- 533
           TR +AI PSLIVA+IGG  GAG+LIIIAS+I+                            
Sbjct: 368 TRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANSL 427

Query: 534 ----ISWILGLGIIGINIYYLSTGFVGWLIDNNLPKVANVFIGIVVFPLMALYILAVIYL 563
               ++WI+G  I+GINIYYL + F+  L+ +++  VA VF+G++ F  +A Y+ A+ YL
Sbjct: 428 VISSVTWIIGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYL 487

BLAST of Spg008711 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 276.6 bits (706), Expect = 4.9e-74
Identity = 185/463 (39.96%), Postives = 263/463 (56.80%), Query Frame = 0

Query: 135 HEEQTTTHQKPGWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIF 194
           +++      K  W+K     GPGFL+S+A+LDPGNLE+DLQAGA  GY L+W+++     
Sbjct: 37  YDDDPGNSPKFSWKKLWLFTGPGFLMSIAFLDPGNLESDLQAGAIAGYSLIWLLMWATAI 96

Query: 195 ALIIQSLAANLGVST-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNI 254
            L+IQ L+A LGV+T     E+C+ EYP + +  LW++AE+A+I ADI EVIG+A A+ I
Sbjct: 97  GLLIQLLSARLGVATGRHLAELCREEYPTWARMVLWIMAEIALIGADIQEVIGSAIAIKI 156

Query: 255 LFH--IPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPAT 314
           L +  +P+WAGV++T L   + L L+ YG RKLE + A+L+  MA  F       KP  T
Sbjct: 157 LSNGLVPLWAGVVITALDCFIFLFLENYGIRKLEAVFAILIATMALAFAWMFGQTKPSGT 216

Query: 315 GVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKV-PNSVRGINDACRYF 374
            +L G  VPKL+ +     A+ ++G ++MPHN+FLHSALV SR+V P     + +A +Y+
Sbjct: 217 ELLVGALVPKLSSR-TIKQAVGIVGCIIMPHNVFLHSALVQSREVDPKKRFRVKEALKYY 276

Query: 375 LIESGFALFVAFLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSS 434
            IES  AL V+F+INV V +V        +I++T       I L +A   LQ+  G    
Sbjct: 277 SIESTGALAVSFIINVFVTTVFAKSFYGTEIADT-------IGLANAGQYLQDKYGGGFF 336

Query: 435 T---IYAIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVA-- 494
               I+AI +LA+GQSS+ITGTYAGQFIM GFL+LKMK WVR L+TRS AI P++IVA  
Sbjct: 337 PILYIWAIGVLAAGQSSTITGTYAGQFIMGGFLNLKMKKWVRALITRSCAIIPTMIVALV 396

Query: 495 --------------------------------IIGGPQGAGRLIIIASIIVISWILGLGI 553
                                           ++   Q  G   I   +  ISWI+   +
Sbjct: 397 FDSSDSMLDELNEWLNVLQSVQIPFAVIPLLCLVSNEQIMGSFKIQPLVQTISWIVAALV 456

BLAST of Spg008711 vs. TAIR 10
Match: AT2G23150.1 (natural resistance-associated macrophage protein 3 )

HSP 1 Score: 271.9 bits (694), Expect = 1.2e-72
Identity = 161/348 (46.26%), Postives = 223/348 (64.08%), Query Frame = 0

Query: 147 WRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAANLG 206
           W+K     GPGFL+S+A+LDPGNLE DLQAGA  GY LLW+++      L++Q L+A LG
Sbjct: 53  WKKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAVAGYSLLWLLMWATAMGLLVQLLSARLG 112

Query: 207 VST-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFH--IPVWAGV 266
           V+T     E+C+ EYP + +  LW++AE+A+I +DI EVIG+A A+ IL +  +P+WAGV
Sbjct: 113 VATGRHLAELCRDEYPTWARMVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAGV 172

Query: 267 LLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPKLN 326
           ++T L   + L L+ YG RKLE + AVL+  M   F       KP  + +L G+ VPKL+
Sbjct: 173 VITALDCFVFLFLENYGIRKLEAVFAVLIATMGVSFAWMFGQAKPSGSELLIGILVPKLS 232

Query: 327 GQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVR-GINDACRYFLIESGFALFVAF 386
            +     A+ ++G ++MPHN+FLHSALV SR+V    +  + +A  Y+ IES  ALF++F
Sbjct: 233 SR-TIQKAVGVVGCIIMPHNVFLHSALVQSREVDKRQKYRVQEALNYYTIESTIALFISF 292

Query: 387 LINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSST---IYAIALLA 446
           LIN+ V        TV        D  + I L +A   LQ   G        I+AI LLA
Sbjct: 293 LINLFVT-------TVFAKGFYNTDLANSIGLVNAGQYLQEKYGGGVFPILYIWAIGLLA 352

Query: 447 SGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAII 484
           +GQSS+ITGTYAGQFIM GFL+ KMK W+R L+TRS AI P++IVA++
Sbjct: 353 AGQSSTITGTYAGQFIMGGFLNFKMKKWLRALITRSCAIIPTIIVALV 392

BLAST of Spg008711 vs. TAIR 10
Match: AT1G47240.1 (NRAMP metal ion transporter 2 )

HSP 1 Score: 265.8 bits (678), Expect = 8.7e-71
Identity = 173/410 (42.20%), Postives = 243/410 (59.27%), Query Frame = 0

Query: 87  LKREREEMASRQKEAQMSTAATPSFGGGSNRIAAVTTTPANAVKPQNHHEEQTTTHQKP- 146
           +K   EE   R       + + PS    S   AA  T     +      ++ TT    P 
Sbjct: 5   VKENLEEEEDRLLPPPPPSQSLPSTDSESE--AAFETNEKILIVDFESPDDPTTGDTPPP 64

Query: 147 -GWRKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGYELLWVVLIGLIFALIIQSLAAN 206
             WRK     GPGFL+S+A+LDPGNLE DLQAGA  GY LLW+++      L+IQ L+A 
Sbjct: 65  FSWRKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQMLSAR 124

Query: 207 LGVST-----EVCKAEYPKFVKYFLWLLAEVAVIAADIPEVIGTAFALNILFH--IPVWA 266
           +GV+T     E+C+ EYP + +Y LW +AE+A+I ADI EVIG+A A+ IL    +P+WA
Sbjct: 125 VGVATGRHLAELCRDEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLPLWA 184

Query: 267 GVLLTGLSTLLLLGLQRYGARKLELLIAVLVFVMAACFFGEMSYVKPPATGVLKGMFVPK 326
           GV++T     L L L+ YG RKLE + AVL+  M   F       KP    ++ G+ +P+
Sbjct: 185 GVVITASDCFLFLFLENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGILLPR 244

Query: 327 LNGQGATADAIALLGALVMPHNLFLHSALVLSRKV-PNSVRGINDACRYFLIESGFALFV 386
           L+ +     A+ ++G ++MPHN+FLHSALV SRK+ P     + +A  Y+LIES  ALF+
Sbjct: 245 LSSK-TIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESSVALFI 304

Query: 387 AFLINVAVVSVSGTVCTVGKISETTADQCSDITLNSASFLLQNVLGKSSST---IYAIAL 446
           +F+IN+ V     TV   G       ++ ++I L +A   LQ   G        I+ I L
Sbjct: 305 SFMINLFVT----TVFAKGFYG---TEKANNIGLVNAGQYLQEKFGGGLLPILYIWGIGL 364

Query: 447 LASGQSSSITGTYAGQFIMQGFLDLKMKTWVRNLMTRSIAITPSLIVAII 484
           LA+GQSS+ITGTYAGQFIM GFL+L++K W+R ++TRS AI P++IVAI+
Sbjct: 365 LAAGQSSTITGTYAGQFIMGGFLNLRLKKWMRAVITRSCAIVPTMIVAIV 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023526032.11.2e-25887.07metal transporter Nramp5-like [Cucurbita pepo subsp. pepo][more]
XP_022953164.11.5e-25887.25metal transporter Nramp5-like [Cucurbita moschata][more]
XP_038886296.12.4e-25686.74metal transporter Nramp5-like [Benincasa hispida][more]
XP_022991146.13.5e-25586.18metal transporter Nramp5-like [Cucurbita maxima][more]
XP_011658448.25.7e-25086.13metal transporter Nramp5 [Cucumis sativus] >KGN47700.1 hypothetical protein Csa_... [more]
Match NameE-valueIdentityDescription
Q8H4H59.7e-18467.79Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2... [more]
Q0D7E42.8e-16767.25Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2... [more]
Q6ZG852.8e-15155.53Metal transporter NRAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAT1 PE=2 S... [more]
Q9SAH82.0e-14157.23Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1[more]
Q653V61.7e-14054.71Metal transporter Nramp3 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP3 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1GNU97.3e-25987.25metal transporter Nramp5-like OS=Cucurbita moschata OX=3662 GN=LOC111455783 PE=3... [more]
A0A6J1JPX41.7e-25586.18metal transporter Nramp5-like OS=Cucurbita maxima OX=3661 GN=LOC111487830 PE=3 S... [more]
A0A0A0KDB82.8e-25086.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G382880 PE=3 SV=1[more]
A0A1S3CA247.8e-24585.04metal transporter Nramp5-like OS=Cucumis melo OX=3656 GN=LOC103498358 PE=3 SV=1[more]
A0A6J1CJ542.1e-24283.63metal transporter Nramp5-like OS=Momordica charantia OX=3673 GN=LOC111011923 PE=... [more]
Match NameE-valueIdentityDescription
AT1G80830.11.5e-14257.23natural resistance-associated macrophage protein 1 [more]
AT1G15960.13.6e-14156.58NRAMP metal ion transporter 6 [more]
AT5G67330.14.9e-7439.96natural resistance associated macrophage protein 4 [more]
AT2G23150.11.2e-7246.26natural resistance-associated macrophage protein 3 [more]
AT1G47240.18.7e-7142.20NRAMP metal ion transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001046NRAMP familyPRINTSPR00447NATRESASSCMPcoord: 224..250
score: 57.33
coord: 465..482
score: 57.11
coord: 327..350
score: 63.0
coord: 252..271
score: 44.0
coord: 277..298
score: 41.64
coord: 437..456
score: 73.0
IPR001046NRAMP familyTIGRFAMTIGR01197TIGR01197coord: 148..507
e-value: 2.4E-106
score: 354.2
IPR001046NRAMP familyPFAMPF01566Nrampcoord: 171..499
e-value: 6.8E-98
score: 327.9
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 140..500
coord: 498..609
IPR001046NRAMP familyHAMAPMF_00221NRAMPcoord: 131..521
score: 33.353912
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..74
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 99..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..68
NoneNo IPR availablePANTHERPTHR11706:SF77METAL TRANSPORTER NRAMP5coord: 140..500
coord: 498..609

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg008711.1Spg008711.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070574 cadmium ion transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:0030001 metal ion transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015086 cadmium ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity