Spg008659 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg008659 vs. NCBI nr
Match: KAG5584663.1 (hypothetical protein H5410_045097 [Solanum commersonii])

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-31
Identity = 102/325 (31.38%), Postives = 166/325 (51.08%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G+KIQA +FN+ IET  + +  NK Y I  G +  VN  Y++V++E+E+T   +T I+E 
Sbjct: 80  GTKIQATLFNKHIETWKDSLKPNKRYYIAKGRLDRVNPNYSSVHKEVELTFIDNTIIDET 139

Query: 105 EEPFPLHKLTYNFIQISEMESFGMTGKMIGELSMATTMTSNILIEPDFVDSKDLKERREL 164
                  K +  F+ +   +     G ++G   ++T   S+ILI   F  + DL+  RE+
Sbjct: 140 SHEVSTLKFSDGFVSLDHADKL-PNGAILGTFGISTIPVSSILINLMFQKAMDLRAWREI 199

Query: 165 LLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVDDLYCCII---GSIVEIVNKDNPWFEK 224
           +         + PS+ M RA  V +  I+ GL+ D    +      I++++N D PW+  
Sbjct: 200 IKAEN-KDIIVTPSRVMRRAIKVPLPHILDGLLADSQDSMYKFKEKIIDVINIDEPWYSS 259

Query: 225 CKLCNQRVYPNGSQKMAHCNNCN-DLVQFIRKYMLKLKICDGQMTAHITAFEAAEVIVGS 284
           CK C+++V      +   C NCN + V++  +Y L+L++C G+  A +  FEA + ++G 
Sbjct: 260 CKKCHKKV--TFIDETTACTNCNIENVEYEMRYCLRLEMCGGEQRARVILFEATKFLLGC 319

Query: 285 SVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHFVVQEINKVE 344
           +V  Y        KKE+ K YR  +L + K Y FLVR+DK         + + QEI  +E
Sbjct: 320 TVQEYIQS--TSVKKEECKNYRKLVLTKDKEYNFLVRIDK--NEPNPPRNLIAQEIYPLE 379

Query: 345 VEE----SDLETLEVLHVKKKIKIE 362
            E      D E L  L  K+  KI+
Sbjct: 380 DEAPTIVEDEEKLAPLGRKRTKKIK 396

BLAST of Spg008659 vs. NCBI nr
Match: XP_015160730.1 (PREDICTED: replication protein A 70 kDa DNA-binding subunit D-like [Solanum tuberosum])

HSP 1 Score: 137.5 bits (345), Expect = 2.3e-28
Identity = 84/299 (28.09%), Postives = 153/299 (51.17%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G+KIQ  +FN+ +         N+ Y I NG +      + +V++++E+   ++T + E 
Sbjct: 58  GTKIQTTLFNKDVHAWNKSFQLNQSYYIINGKLNGAKPNFLSVHKDLELAFMNNTDVVED 117

Query: 105 EEPFPLHKLTYNFIQISEMESFGMTGKMIGELSMATTMTSNILIEPDFVDSKDLKERREL 164
           +  F   + +  FI   E E     G + G+  ++TT  S++LI P F  + +L+E  + 
Sbjct: 118 KSQFKTEQFSNGFISFDEAEKI-TNGSLFGDFVLSTTPVSSLLINPQFEKANNLQEWNDN 177

Query: 165 LLDHTYMSTFIPPSKKMERAKLVSIKDIIHG---LVDDLYCCIIGSIVEIVNKDNPWFEK 224
           +     +   + PS+ M+ A+ V I +I++G   +V D+Y      +  I N  +PW+  
Sbjct: 178 MKAQK-IDLSLMPSRLMQTARQVKITNILNGSLAIVKDMYYKFNAMVSGIDNNTDPWYHA 237

Query: 225 CKLCNQRVYPNGSQKMAHCNNC-NDLVQFIRKYMLKLKICDGQMTAHITAFEAAEVIVGS 284
           CK C +RV    S   A C  C  + + +  +Y LK+ + D      IT F+AA+  +GS
Sbjct: 238 CKKCYKRVTVINSN--ATCTYCRTEDIDYEARYRLKIDVTDEDQFLRITLFDAAKYFLGS 297

Query: 285 SVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHFVVQEINKV 340
            V  Y   +   +KKE+S+ YR  +L++GK +  LV++D+ F    +  + +  EI++V
Sbjct: 298 HVKDYV--LSTSEKKEESEYYRKLVLSKGKEFSILVKIDRNFPNVEANMNVIAMEIHEV 350

BLAST of Spg008659 vs. NCBI nr
Match: XP_020262698.1 (uncharacterized protein LOC109838687 [Asparagus officinalis])

HSP 1 Score: 136.0 bits (341), Expect = 6.6e-28
Identity = 114/400 (28.50%), Postives = 177/400 (44.25%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G KIQ +MF E IE   + + +N    I NG VK +N  Y NV+E IE+TL+ + +I+E 
Sbjct: 559 GEKIQGIMFGETIEMYKHMLQKNNVLYISNGEVKPINPLYGNVHESIELTLTKNISIKES 618

Query: 105 EEPFPLHKLTYNFIQISEMES--------------------------------------- 164
           +  F   K+  NFI I +  S                                       
Sbjct: 619 KAEFQFDKILNNFISIKDAISNEDTRNINVLAMIANVKPMIKYVTRYNKADTRRDIIIVD 678

Query: 165 ----------FG----MTGKMI---------------------GELSMATTMTSNILIEP 224
                     FG      G MI                     GEL + + + S I I P
Sbjct: 679 KNDITLSVTLFGDLAVNEGSMIEGTKHETTIIALSDFKISLYKGELCLTSQIASTIFINP 738

Query: 225 DFVDSKDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVDD---LYCCIIGS 284
           D   +KD+   RE  L    + T   PS+  + A  ++I DI+    +     Y   IG 
Sbjct: 739 DIQLAKDM---REWALSKENIKTNDAPSRLFKNATEMNIGDIMQASQESSKAQYGSFIGK 798

Query: 285 IVEIVNKDNPWFEKCKLCNQRVYPNGSQKMAHCNNCNDLVQFIRKYMLKLKICDGQMTAH 344
           I  I+N+   W+  C  CN++V  N   K  +C   N+    I +Y++K+ + D    A 
Sbjct: 799 ISNILNRHEAWYYSCTGCNKKVDKNIYDKCQNCQKNNE--DSIPRYVVKIIVEDEIDNAR 858

Query: 345 ITAFEAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRS 363
           +T F+AAE ++G     +   I   +KK  S  ++ F+L  GKTYKFLV++D++ + +R+
Sbjct: 859 VTLFDAAETLIGCDAQKFMDDI--KEKKNQSSFFQKFVLNIGKTYKFLVKLDERSKEERA 918

BLAST of Spg008659 vs. NCBI nr
Match: KAG5574299.1 (hypothetical protein H5410_054433 [Solanum commersonii])

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-27
Identity = 109/391 (27.88%), Postives = 176/391 (45.01%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G+KIQA +FN+ IET  + +  NK Y I  G +  VN  Y++V++E+E+  + +T I+E 
Sbjct: 33  GTKIQATLFNKHIETWKDSLKPNKSYYIAKGRLDRVNPNYSSVHKEVELAFTDNTIIQET 92

Query: 105 EEPFPLHKLTYNFIQISE---------------------------------------MES 164
           E     HK +  F+ +                                         ME 
Sbjct: 93  EHAVSTHKFSDGFLSLEHADKLPNGAILDLICVLVSVNPLIQNVNFKRREIVVTNQFMEH 152

Query: 165 FGMT---------GKMI---------------------GELSMATTMTSNILIEPDFVDS 224
             +T         G  +                     G   ++T   S++LI P F  +
Sbjct: 153 TSVTLWGDFAENDGSFLEKLQDDKPILALCDVRVSIFKGRFGISTIPVSSVLINPMFQKA 212

Query: 225 KDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVDDLYCCII---GSIVEIV 284
            DL+  RE++       T I P+K M+RA  V +  I+ G   D   C+     +I +I+
Sbjct: 213 NDLRAWREIIKADNKDLT-ITPTKVMKRAIEVPLAKILAGFSADSEDCMYKFKATIKDIL 272

Query: 285 NKDNPWFEKCKLCNQRVYPNGSQKMAHCNNCN-DLVQFIRKYMLKLKICDGQMTAHITAF 344
           NKD PW+  CK C+++V     +  A CNNCN D V++  +Y L+L++C+G+  A +  F
Sbjct: 273 NKDEPWYSACKKCHKKV--QFIEDTAACNNCNSDNVEYKMRYCLRLEVCNGERRARVILF 332

Query: 345 EAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHF 360
           EAA+ ++G +V  Y        KK++   YR  +L++   + FLVR+D      R     
Sbjct: 333 EAAKYLLGCNVQEYIES--NSVKKKECYYYRKLVLSKDNQFNFLVRIDMNDPNPRRS--L 392

BLAST of Spg008659 vs. NCBI nr
Match: XP_020242959.1 (replication protein A 70 kDa DNA-binding subunit B-like [Asparagus officinalis])

HSP 1 Score: 134.0 bits (336), Expect = 2.5e-27
Identity = 93/340 (27.35%), Postives = 170/340 (50.00%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G+ IQA++FN++IE     +++ + Y I NG VK  N KY  +N E E+TL++ T I E 
Sbjct: 67  GTLIQAILFNKLIEKFSGLLIKEETYTITNGEVKPPNLKYPTINPEKELTLTTKTIIRET 126

Query: 105 EEPFPLHKLTYNFIQISEMESFG---MTGKMIG-------------------------EL 164
           ++   L KL   F+   ++ +F    M   ++G                          +
Sbjct: 127 KKIVSLEKLKPTFLSFKDVSAFDDGIMPFDVVGFVITVKPIFTNPKSKRREVIIMNKERV 186

Query: 165 SMATTMTSNILIEPDFVDSKDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIH-- 224
           S+ T  ++ +L+ P+     ++ +  +     +  S  +  +K++ +A  V + D+I+  
Sbjct: 187 SITTKSSTLVLVNPEIPQCSEMIDWLKNSNSESSKSVLLTTTKRITQANRVKLIDVINTS 246

Query: 225 -GLVDDLYCCIIGSIVEIVNKDNPWFEKCKLCNQRVYP-NGSQKMAHCNNC-NDLVQFIR 284
             L   ++C  +  I+ I+N + P+F  C  C + V+  NGS   A+C NC N    +  
Sbjct: 247 PQLSKSMFCSFVAKIITIINAEEPFFSACTKCKKAVHEINGS--TANCTNCKNGNSDWTI 306

Query: 285 KYMLKLKICDGQMTAHITAFEAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKT 344
           +Y L+L++ DG  TA++T F+ AE+++G +   Y    ++M + E S+ Y+       K 
Sbjct: 307 RYNLRLEVNDGTDTAYVTLFDEAEILIGCTAIAY----VEMFEDESSECYQKMKSCLEKE 366

Query: 345 YKFLVRMDKKFEGDRSKGHFVVQEINKV-EVEESDLETLE 351
           Y FLV++++K E +R     + QE+ KV E+    +ET E
Sbjct: 367 YVFLVKINEKEESNRKARCIIAQEVRKVEEIHPPTVETEE 400

BLAST of Spg008659 vs. ExPASy Swiss-Prot
Match: Q9FME0 (Replication protein A 70 kDa DNA-binding subunit D OS=Arabidopsis thaliana OX=3702 GN=RPA1D PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.1e-04
Identity = 42/192 (21.88%), Postives = 92/192 (47.92%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G++IQA MFN+      +     K Y I  G++K  N ++  V  + EMTL+ ++ +EE 
Sbjct: 228 GTQIQATMFNDAARKFFDRFQLGKVYYISRGSLKLANKQFKTVQNDYEMTLNENSEVEEA 287

Query: 105 --EEPFPLHKLTYNFIQISEMESFGMTGKMIGEL----SMATTMTSNILIEPDFVDSKDL 164
             EE F + +  +NF+ I E+  +    ++I  +    S++ TM+     + + +  +D+
Sbjct: 288 SSEEMF-IPETKFNFVPIEELGLYVNQKELIDLIGVVQSVSPTMSIRRRTDNEMIPKRDI 347

Query: 165 ---KERRELLLDHTYMSTFIPPSKKM----ERAKLVSIKDIIHGLVDDLYCCIIGSIVEI 224
               E R+ ++   +        +++    +++ +++IK +  G    +    I     +
Sbjct: 348 TLADESRKTVVVSLWNDLATGIGQELLDMADQSPVIAIKSLKVGDFQGVSLSTISRSNVV 407

BLAST of Spg008659 vs. ExPASy TrEMBL
Match: A0A1S4BAI4 (replication protein A 70 kDa DNA-binding subunit B-like OS=Nicotiana tabacum OX=4097 GN=LOC107806315 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 8.7e-26
Identity = 98/369 (26.56%), Postives = 162/369 (43.90%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G+KI A +FN  IE   N + +NK Y I NG + + N  + +V+ E+E+    +T +EE 
Sbjct: 58  GTKIHATLFNGYIELWKNYLQQNKIYYIINGRINSANPNFQSVHNELEIGFKENTLVEES 117

Query: 105 EEPFPLHKLTYNFIQISEMES------FGMTGKMI------------------------- 164
              F     + NFI   E         F + G ++                         
Sbjct: 118 NSHFSTIGFSNNFISFDEASKCTNGTIFDIVGILVDIKPSMGEGSKKRREIVLINEMVDR 177

Query: 165 --------------------------------------GELSMATTMTSNILIEPDFVDS 224
                                                 G  S++TT  S+I+I P F  +
Sbjct: 178 KTVTLWNDFSEIDGQVLQKLIDEKPVVAACDVRKTSYRGNFSISTTYVSSIMINPKFEKA 237

Query: 225 KDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVD---DLYCCIIGSIVEIV 284
             L++  +       +   + PS +++ A+ V I+DI     D   D Y      I +I+
Sbjct: 238 VALQKWHDYKKAKN-IDISLLPSIQLKNAREVKIEDIKDDSFDNKEDTYFKFNARIKDIM 297

Query: 285 NKDNPWFEKCKLCNQRVYPNGSQKMAHCNNCN-DLVQFIRKYMLKLKICDGQMTAHITAF 341
           NKD PW+  CK C ++V  +    +A C++C  + V +  +Y+LKL++ D +   ++T F
Sbjct: 298 NKDEPWYSSCKKCYKKV--DVINNIAKCHHCGVENVDYQERYILKLEVFDKKERCYVTLF 357

BLAST of Spg008659 vs. ExPASy TrEMBL
Match: A0A1S4AI89 (replication protein A 70 kDa DNA-binding subunit B-like OS=Nicotiana tabacum OX=4097 GN=LOC107797997 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.3e-25
Identity = 95/342 (27.78%), Postives = 152/342 (44.44%), Query Frame = 0

Query: 39  VCNLGMGSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSS 98
           V +   G+KI A +FN  IE   N + +NK   I NG + + N  + +V+ E+E+    S
Sbjct: 42  VSDSSWGTKIHATLFNGYIELWKNYLQQNKICYIINGRINSANPNFQSVHNELEIGFKES 101

Query: 99  TTIEEIEEPFPLHKLTYNFIQISEMES------FGMTGKMI------------------- 158
           T +EE    F     + NFI   E         FG+  K +                   
Sbjct: 102 TLVEESNSHFSTIGFSNNFISFDEASKCTNGTIFGLIEKTVTLWNDFSEINGQVLQKLID 161

Query: 159 ----------------GELSMATTMTSNILIEPDFVDSKDLKERRELLLDHTYMSTFIP- 218
                           G  S++TT  S I+I P F  +  L++  +         + +P 
Sbjct: 162 EKPVVATCDIRKTSYRGNFSISTTYVS-IMINPKFEKAVALQKWHDYKKAKNIDISSLPN 221

Query: 219 -PSKKMERAKLVSIKDIIHGLVDDLYCCIIGSIVEIVNKDNPWFEKCKLCNQRVYPNGSQ 278
              K     K+  +KD      +D Y      I +I+NKD PW+  CK C ++V  +   
Sbjct: 222 IQLKNAREVKIEDMKDDSFDNKEDTYFKFNARIKDIMNKDEPWYSSCKKCYKKV--DVIN 281

Query: 279 KMAHCNNCN-DLVQFIRKYMLKLKICDGQMTAHITAFEAAEVIVGSSVSHYATQILQMKK 337
            +  C+ C  + V +  +Y+LKL++ D +   ++T FE+   ++G  V+ Y   I Q   
Sbjct: 282 NITKCHRCGVENVDYQERYILKLEVFDKKERYYVTLFESTRYLLGCDVTTYIQSISQ--N 341

BLAST of Spg008659 vs. ExPASy TrEMBL
Match: M1DXI7 (Replication protein A1 OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-24
Identity = 100/386 (25.91%), Postives = 172/386 (44.56%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G+KIQ  +FN+ IET  + +  NK Y I  G    VN  Y++V++E+E+  + +T I+E 
Sbjct: 58  GTKIQTTLFNKHIETWKDFLKPNKSYYIAKGRYDRVNPNYSSVHKEVELAFTDNTVIKET 117

Query: 105 EEPFPLHKLTYNFIQI-----------------------------SEMESFGMTGKMI-- 164
           +      K +  F+ +                             S+     +T +++  
Sbjct: 118 DHEVSTQKFSDGFLSLDAADNLPNGSVLDLICVLVSVNPIIQNATSKRREIVVTNELMEP 177

Query: 165 --------------------------------------GELSMATTMTSNILIEPDFVDS 224
                                                 G   ++T   S++LI P F  +
Sbjct: 178 TTVTLWGDFAENDGAFLDKLKDDKPILGLCDVRVSIYKGRFGISTIPVSSVLINPIFQKA 237

Query: 225 KDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLV----DDLYCCIIGSIVEI 284
            DL+  RE +++       + P+K M+RA  V +  I++GL+    D +Y     +IV+I
Sbjct: 238 LDLRAWRE-IIEADNKDIIVAPTKAMKRAIEVPLDHILNGLLAESQDSMY-KFKATIVDI 297

Query: 285 VNKDNPWFEKCKLCNQRVYPNGSQKMAHCNNCN-DLVQFIRKYMLKLKICDGQMTAHITA 344
           +NKD PW+  CK C ++V     ++ A C NCN D V++  +Y L+L++C G+  A +  
Sbjct: 298 LNKDEPWYLSCKTCRKKV--KVIKEAAACTNCNIDNVEYAMRYCLRLEVCGGEKRAQVVL 357

Query: 345 FEAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGH 357
           FEAA  ++G              KKE+   YRN +L++ K + FLVR+D      R    
Sbjct: 358 FEAANNLLGC-------------KKEECHYYRNLVLSKDKQFNFLVRIDLTDANPRRS-- 417

BLAST of Spg008659 vs. ExPASy TrEMBL
Match: M1DE88 (Replication protein A1 OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.4e-23
Identity = 74/247 (29.96%), Postives = 132/247 (53.44%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G+KIQA +FN+ IET  + + RNK Y I  G + ++N  Y++V++E+E+T + +T I+E 
Sbjct: 98  GTKIQATLFNKHIETWKDSLKRNKSYYIAKGWLDSINPNYSSVHKEVELTFTDNTIIQET 157

Query: 105 EEPFPLHKLTYNFIQISEMESFGMTGKMIGELSMATTMTSNILIEPDFVDSKDLKERREL 164
                 HK +  F+ +   +     G ++  +         +L+  + V      +RRE+
Sbjct: 158 THAVSTHKFSDGFLSLEHADKL-PNGSILDLIC--------VLVSVNPVVQNANSKRREI 217

Query: 165 LLDHTYMSTF--------IPPSKKMERAKLVSIKDIIHGLVDDLYCCII---GSIVEIVN 224
           ++ +              I P++ M RA  V++ +I+ GL  D   C+     +I +I+N
Sbjct: 218 VVTNQLREIIKADNKDITIMPTEVMRRAIEVTLDNILDGLFADSEDCMYKFKATIEDILN 277

Query: 225 KDNPWFEKCKLCNQRVYPNGSQKMAHCNNCNDL-VQFIRKYMLKLKICDGQMTAHITAFE 280
           KD PW+  CK C+++V     +  A CNNCN   V++  +Y ++L++CDG+  A +  FE
Sbjct: 278 KDEPWYSSCKKCHKKV--RYIEDTATCNNCNSQNVEYEMRYCVRLEVCDGERRAKVILFE 333

BLAST of Spg008659 vs. ExPASy TrEMBL
Match: M1DP79 (Replication factor A OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.1e-23
Identity = 100/398 (25.13%), Postives = 172/398 (43.22%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G K+QA +FN+ I+T  + +  NK Y I  G +  VN  Y +V++EIE+T++ +T I++ 
Sbjct: 33  GIKLQATLFNKHIDTWKDFLKTNKTYYIAKGLLDRVNPNYTSVHKEIELTITDNTIIKDS 92

Query: 105 EEPFPLHKLTYNFIQISEMESFG-----------------------------MTGKMI-- 164
           +     H  +  F+ +   E                                +T +++  
Sbjct: 93  DHVVSTHNFSNGFVSLEHAEKLPNGAIFDLICVLVTVNPIIVKGDSKRREIVVTNELMEC 152

Query: 165 --------------------------------------GELSMATTMTSNILIEPDFVDS 224
                                                 G   ++T   S++LI P F  +
Sbjct: 153 TTVTLWGDFAENDGAFLEKLKDDQPILGLCDVRVSIYKGTFGISTIPVSSVLINPIFQKA 212

Query: 225 KDLKERRELLLDHTYMSTFIPPSKKMERAKLVSIKDIIHGLVDDLYCCII---GSIVEIV 284
            DL+  RE++       T   PSK M +A  V++ +I+ G + D    +      IV+I+
Sbjct: 213 LDLRAWREVIKAEKKPIT-TTPSKVMRKATEVTLLNIMDGTLADTQEALYKFKAKIVDIL 272

Query: 285 NKDNPWFEKCKLCNQRVYPNGSQKMAHCNNCN-DLVQFIRKYMLKLKICDGQMTAHITAF 344
           N + PW+  CK C+++V     Q    C NCN    ++  +Y L+L++CD +  A +T F
Sbjct: 273 NIEEPWYSSCKQCHKKV--QVKQDTVTCPNCNIQNGEYEMRYSLRLEVCDEEQRARVTLF 332

Query: 345 EAAEVIVGSSVSHYATQILQMKKKEDSKIYRNFILAQGKTYKFLVRMDKKFEGDRSKGHF 363
           +AA++++G +V  Y       +K E+S  YRN + ++ K +KFLVR+D            
Sbjct: 333 DAAQMLLGCTVEDYIK--TTSEKNEESMYYRNLVFSKDKEFKFLVRID--MNNSNPCRSL 392

BLAST of Spg008659 vs. TAIR 10
Match: AT5G61000.1 (Replication factor-A protein 1-related )

HSP 1 Score: 47.8 bits (112), Expect = 2.2e-05
Identity = 42/192 (21.88%), Postives = 92/192 (47.92%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G++IQA MFN+      +     K Y I  G++K  N ++  V  + EMTL+ ++ +EE 
Sbjct: 228 GTQIQATMFNDAARKFFDRFQLGKVYYISRGSLKLANKQFKTVQNDYEMTLNENSEVEEA 287

Query: 105 --EEPFPLHKLTYNFIQISEMESFGMTGKMIGEL----SMATTMTSNILIEPDFVDSKDL 164
             EE F + +  +NF+ I E+  +    ++I  +    S++ TM+     + + +  +D+
Sbjct: 288 SSEEMF-IPETKFNFVPIEELGLYVNQKELIDLIGVVQSVSPTMSIRRRTDNEMIPKRDI 347

Query: 165 ---KERRELLLDHTYMSTFIPPSKKM----ERAKLVSIKDIIHGLVDDLYCCIIGSIVEI 224
               E R+ ++   +        +++    +++ +++IK +  G    +    I     +
Sbjct: 348 TLADESRKTVVVSLWNDLATGIGQELLDMADQSPVIAIKSLKVGDFQGVSLSTISRSNVV 407

BLAST of Spg008659 vs. TAIR 10
Match: AT5G08020.1 (RPA70-kDa subunit B )

HSP 1 Score: 44.7 bits (104), Expect = 1.9e-04
Identity = 34/127 (26.77%), Postives = 63/127 (49.61%), Query Frame = 0

Query: 45  GSKIQAVMFNEVIETIGNGILRNKFYLIENGNVKNVNGKYANVNEEIEMTLSSSTTIEEI 104
           G++IQA MFN       +     K Y I  G++K  N ++  V  + EMTL+ ++ +EE 
Sbjct: 204 GTQIQATMFNAAARKFYDRFEMGKVYYISRGSLKLANKQFKTVQNDYEMTLNENSEVEEA 263

Query: 105 --EEPFPLHKLTYNFIQISEMESFGMTGKMIGELSMATTMTSNILIEPDFVDSKDLKERR 164
             EE F   +  +NF+ I E+ ++     +I  + +  +++  + I     D++ + +R 
Sbjct: 264 SNEEMF-TPETKFNFVPIDELGTYVNQKDLIDVIGVVQSVSPTMSIRRK-NDNEMIPKRD 323

Query: 165 ELLLDHT 170
             L D T
Sbjct: 324 ITLADET 328