Spg008645 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg008645
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	NEDD8-specific protease 1-like
Location	scaffold10: 32014865 .. 32015542 (-)
RNA-Seq Expression	Spg008645
Synteny	Spg008645

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATACCACGTTCTTGACTTACAACGATGTCGTGCTTCGAAGATCCGATTTGCATAGTTTGGAAGGTCGTAACTATTTGAACGACACCATCATCGATTTCTATTTCACCTATCTCTCTACATGGTACCAATCTCACGAAATCCTATTGGTTCCAACGAGTATCTCGTTTTTGCTAGCCAACACTCTTGATTGGGATGTTTTCATAGCCACAATCGAATCCCTGAACTTCCATGGAAAGAAACTCATCATCTTCACTGTGAATGACAACTCTAATTTTTCATCATCTGGTGGAGGATCTCATTGGAGCTTGCTTGTGTATTGTAGAAAGCGGAATTTGTTCATGCATTATGATAGTCTTCACATGATGAACTATGAACCTGCTTTCTCTATCTATGAATCTGTTAATAGCTACGTTAGTTATGATTCGGCTGAGTTCATAGAGTGTCGTGCGCCTCGACAAAGGAATGGATATGATTGTGGACTTTACGTGATGGAAATTGCTAGGGTGATATGTGAATGTTATTTTAATGTTGATCAATTTCGAAGCGAGGATGAGATATGTGGATCCTTTAATGTGATGCGAAGTGTGAATTGCAACTACGTTGAGTCCAACATGCGTTCTGAATTGTTGCAGCTGATATCGAGATTGAAGCAAGAAGAAACCAACTCTAAATAG

mRNA sequence

ATGGATACCACGTTCTTGACTTACAACGATGTCGTGCTTCGAAGATCCGATTTGCATAGTTTGGAAGGTCGTAACTATTTGAACGACACCATCATCGATTTCTATTTCACCTATCTCTCTACATGGTACCAATCTCACGAAATCCTATTGGTTCCAACGAGTATCTCGTTTTTGCTAGCCAACACTCTTGATTGGGATGTTTTCATAGCCACAATCGAATCCCTGAACTTCCATGGAAAGAAACTCATCATCTTCACTGTGAATGACAACTCTAATTTTTCATCATCTGGTGGAGGATCTCATTGGAGCTTGCTTGTGTATTGTAGAAAGCGGAATTTGTTCATGCATTATGATAGTCTTCACATGATGAACTATGAACCTGCTTTCTCTATCTATGAATCTGTTAATAGCTACGTTAGTTATGATTCGGCTGAGTTCATAGAGTGTCGTGCGCCTCGACAAAGGAATGGATATGATTGTGGACTTTACGTGATGGAAATTGCTAGGGTGATATGTGAATGTTATTTTAATGTTGATCAATTTCGAAGCGAGGATGAGATATGTGGATCCTTTAATGTGATGCGAAGTGTGAATTGCAACTACGTTGAGTCCAACATGCGTTCTGAATTGTTGCAGCTGATATCGAGATTGAAGCAAGAAGAAACCAACTCTAAATAG

Coding sequence (CDS)

ATGGATACCACGTTCTTGACTTACAACGATGTCGTGCTTCGAAGATCCGATTTGCATAGTTTGGAAGGTCGTAACTATTTGAACGACACCATCATCGATTTCTATTTCACCTATCTCTCTACATGGTACCAATCTCACGAAATCCTATTGGTTCCAACGAGTATCTCGTTTTTGCTAGCCAACACTCTTGATTGGGATGTTTTCATAGCCACAATCGAATCCCTGAACTTCCATGGAAAGAAACTCATCATCTTCACTGTGAATGACAACTCTAATTTTTCATCATCTGGTGGAGGATCTCATTGGAGCTTGCTTGTGTATTGTAGAAAGCGGAATTTGTTCATGCATTATGATAGTCTTCACATGATGAACTATGAACCTGCTTTCTCTATCTATGAATCTGTTAATAGCTACGTTAGTTATGATTCGGCTGAGTTCATAGAGTGTCGTGCGCCTCGACAAAGGAATGGATATGATTGTGGACTTTACGTGATGGAAATTGCTAGGGTGATATGTGAATGTTATTTTAATGTTGATCAATTTCGAAGCGAGGATGAGATATGTGGATCCTTTAATGTGATGCGAAGTGTGAATTGCAACTACGTTGAGTCCAACATGCGTTCTGAATTGTTGCAGCTGATATCGAGATTGAAGCAAGAAGAAACCAACTCTAAATAG

Protein sequence

MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLANTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSLHMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQFRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQEETNSK

Homology

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BLAST of Spg008645 vs. NCBI nr
Match: XP_022988745.1 (NEDD8-specific protease 1-like [Cucurbita maxima])

HSP 1 Score: 354.4 bits (908), Expect = 7.4e-94
Identity = 178/219 (81.28%), Postives = 190/219 (86.76%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLA 60
           MD  FLTYN VVLRRSD+ SLE  NYLND IIDFYF YL   YQS +ILLVPTSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSL 120
           N  DWD FIAT+ESLNFH KKLI FTVNDNSNFSSS GGSHWSLLVYCRKRNLFMHYDSL
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSSS-GGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 HMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQ 180
           HMMN E AF+IYESVNSY+SYDSAEF+ECRAPRQRNGYDCGLYVMEIARVI E YFN DQ
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQ 180

Query: 181 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQ 220
           F++EDEICGSFNV++ VNC +VESNMRSE+LQLI  L Q
Sbjct: 181 FQNEDEICGSFNVVQRVNCKHVESNMRSEMLQLILGLNQ 218

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BLAST of Spg008645 vs. NCBI nr
Match: KAG7014407.1 (NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 349.7 bits (896), Expect = 1.8e-92
Identity = 172/223 (77.13%), Postives = 191/223 (85.65%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLA 60
           M   FLTY DVV+R SDLHSLEGRNYLNDTIIDFYF YLS  YQS+EIL +PTS+S+LL 
Sbjct: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60

Query: 61  NTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSL 120
           NTLDWD+FI+TIESLN H KKLI F +NDNSN SSS GGSHWSLLVYCRKRNLFMHYDSL
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSS-GGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 HMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQ 180
             MN EPAF++YESV+ Y++YD AEF+ECRAPRQ NGYDCGLYVME ARVI E YFNVDQ
Sbjct: 121 STMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQ 180

Query: 181 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQEETN 224
           FRS+DEI G+ NVMRSV   YVESNMRSE++QLI RLKQE+ N
Sbjct: 181 FRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQIN 222

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BLAST of Spg008645 vs. NCBI nr
Match: XP_022953908.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 345.1 bits (884), Expect = 4.5e-91
Identity = 171/221 (77.38%), Postives = 188/221 (85.07%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLA 60
           M   FLTY DVV++  DLHSLEGRNYLNDTIIDFYF YLS  YQS+EIL +PTS+SFLL 
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSL 120
           NTLDWD+FI+TIESLN H KKLI F VNDNSN SSS GGSHWSLLVYCRKRNLFMHYDSL
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSSS-GGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 HMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQ 180
             MN EPAF++YESV+ Y++YD AEF+ECRAPRQ NGYDCGLYVME ARVI E YFNVDQ
Sbjct: 121 STMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQ 180

Query: 181 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQEE 222
           FRS+DEI G  NVMRSV   YVESNMRSE++QLI RLKQE+
Sbjct: 181 FRSKDEISGPINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 220

[Close]

BLAST of Spg008645 vs. NCBI nr
Match: XP_022942008.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 341.7 bits (875), Expect = 4.9e-90
Identity = 169/213 (79.34%), Postives = 184/213 (86.38%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLA 60
           MD  FLTYN VVLRRSD+ SLE  NYLND IIDFYF YL   YQS +ILL+ TSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLG 60

Query: 61  NTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSL 120
           N  DWD F+AT+ESLNFH KKLI FTVNDNSNFSSS GGSHWSLLVYCRKRNLFMHYDSL
Sbjct: 61  NASDWDTFVATVESLNFHEKKLIFFTVNDNSNFSSS-GGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 HMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQ 180
           HMMN E AF+IYESVNSY+SYDSAEF+ECRAPRQRN YDCGLYVMEIARVI E YFN DQ
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQ 180

Query: 181 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQL 214
           F++EDEICGSFNV++SVNC +VESNMRSE + +
Sbjct: 181 FQNEDEICGSFNVVQSVNCKHVESNMRSESINI 212

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BLAST of Spg008645 vs. NCBI nr
Match: XP_023549404.1 (NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 335.5 bits (859), Expect = 3.5e-88
Identity = 169/225 (75.11%), Postives = 186/225 (82.67%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLA 60
           M   FLTY DVV++ SDLHSLEGRNYLNDTIIDFYF YLS  YQS+EIL +PTS+SFLL 
Sbjct: 1   MAAKFLTYGDVVIQGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSL 120
           NTLDWD+FI+TIESLN   KKLI F VNDNSN SSS GGSHWSLLVYCRKRNLFMHYDSL
Sbjct: 61  NTLDWDIFISTIESLNLPEKKLIFFVVNDNSNVSSS-GGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 HMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQ 180
             MN E AF++YESVN Y++Y+ AEF+ECR PRQ NGYDCGLYVME ARVI E Y NVDQ
Sbjct: 121 STMNSESAFTLYESVNGYINYEPAEFMECRTPRQNNGYDCGLYVMETARVIYEHYCNVDQ 180

Query: 181 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQEETNSK 226
           FRS+DEI G  NVMRSV   YVE NMRSE+LQLI  LKQE+ NS+
Sbjct: 181 FRSKDEISGPINVMRSVKSKYVEFNMRSEMLQLIHGLKQEQINSR 224

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BLAST of Spg008645 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.2e-38
Identity = 92/223 (41.26%), Postives = 140/223 (62.78%), Query Frame = 0


Query: 2   DTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLAN 61
           D   L+Y DVVLRRSDL  L G  +LND +I+FY ++LST + S  I L+P SI+F ++N
Sbjct: 7   DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66

Query: 62  TLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSLH 121
             D +     ++ LN   K L+I  VNDNSN   + GG HWSLLVY ++ N F+H+DS  
Sbjct: 67  CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126

Query: 122 MMNYEPAFSIYESVNSYVSYDSAEFIECR-APRQRNGYDCGLYVMEIARVICECYFNVDQ 181
            +N   A  ++++V+ +VS   A + EC   P+Q+NGYDCG++++  ARVICE +F+   
Sbjct: 127 GVNRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGG 186

Query: 182 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQEETN 224
            ++ DE+  + NV  +V    + +++R E+L LI +L  E  +
Sbjct: 187 MKNRDELWFA-NVKETV--PDLVNHLREEILALIKKLMSESVS 225

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BLAST of Spg008645 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 7.1e-23
Identity = 73/230 (31.74%), Postives = 121/230 (52.61%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWY----QSHEILLVPTSIS 60
           MD   L+Y D +LR+SD+  L+  N+LND II F F Y ++        H   + P    
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQ 60

Query: 61  FLLANTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMH 120
           F+   +   ++ +  +E L+   K+++   +NDNSN   + GG+HWSLLVY + +N F H
Sbjct: 61  FIKCTSSPAEIAM-FLEPLDLPHKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFH 120

Query: 121 YDSLHMMNYEPAFSIYESVNSYVSY--DSAEFIECRAPRQRNGYDCGLYVMEIARVICEC 180
           YDS    N   A  + E + +++    D   F+E +AP Q+N YDCG+YV+     +C+ 
Sbjct: 121 YDSHSRSNSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQQNSYDCGMYVICNTEALCQ- 180

Query: 181 YFNVDQFRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQEETNS 225
               + FR + E     + ++ +   Y+ +  R E   LI+RL ++  +S
Sbjct: 181 ----NLFRRQPE-----SPLQLLTPTYI-TKKRGEWKDLIARLAKKNRSS 216

[Close]

BLAST of Spg008645 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.2e-22
Identity = 62/182 (34.07%), Postives = 99/182 (54.40%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWY----QSHEILLVPTSIS 60
           MD   L+Y D +LR+SD+  L+  ++LND II F F Y +         H   + P    
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQ 60

Query: 61  FLLANTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMH 120
           F+   +   ++ +  +E L+   K+++   +NDNSN   + GG+HWSLLVY + +N F H
Sbjct: 61  FIKCTSNPAEIAM-FLEPLDLPNKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFH 120

Query: 121 YDSLHMMNYEPAFSIYESVNSYVSY--DSAEFIECRAPRQRNGYDCGLYVMEIARVICEC 177
           YDS    N   A  + E + +++    D   F+E +AP Q+N YDCG+YV+     +C+ 
Sbjct: 121 YDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQN 179

[Close]

BLAST of Spg008645 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 106.3 bits (264), Expect = 4.6e-22
Identity = 62/182 (34.07%), Postives = 97/182 (53.30%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWY----QSHEILLVPTSIS 60
           MD   L+Y D +LR+SD+  L+  ++LND II F F Y +         H   + P    
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQ 60

Query: 61  FLLANTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMH 120
           F+   +   ++ +  +E L+   K+++   +NDNSN   + GG+HWSLLVY + +N F H
Sbjct: 61  FIKCTSSPAEIAM-FLEPLDLPHKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFH 120

Query: 121 YDSLHMMNYEPAFSIYESVNSYVSY--DSAEFIECRAPRQRNGYDCGLYVMEIARVICEC 177
           YDS    N   A  + E + +++    D   F+E +AP Q N YDCG+YV+     +C+ 
Sbjct: 121 YDSHSRSNSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQENSYDCGMYVICNTEALCQS 179

[Close]

BLAST of Spg008645 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.8e-19
Identity = 64/242 (26.45%), Postives = 122/242 (50.41%), Query Frame = 0


Query: 2   DTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQS------HEILLVPTSI 61
           D   ++YND  + +SD++ L+   +LND+II FY  +L    +       +++LL+  S+
Sbjct: 3   DPLIVSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLSPSV 62

Query: 62  SFLLANT-LDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLF 121
            F  +    + ++ +   + L+   K++I F + +N + +  GGG+HWSLL++ +  N F
Sbjct: 63  VFCCSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSLNKF 122

Query: 122 MHYDSLHMMNYEPAFSIYESVNSYVSYDSAE------FIECRAPRQRNGYDCGLYVMEIA 181
           ++YDS++  N   A  I       +S    +       I  + P+Q+NGYDCGLYV+ I 
Sbjct: 123 IYYDSINSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVLSII 182

Query: 182 RVICECYFNVDQFRSEDEICGSFN-----------VMRSVNCNYVESNMRSELLQLISRL 220
             + +     ++    +E   S+            + + +  NY++ + R E+L  I +L
Sbjct: 183 EELLKLIIKENENNKGEENKISYKDLLLSEFTNELLFKEITPNYIK-DKRVEILNTILKL 242

[Close]

BLAST of Spg008645 vs. ExPASy TrEMBL
Match: A0A6J1JKF6 (NEDD8-specific protease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485989 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.6e-94
Identity = 178/219 (81.28%), Postives = 190/219 (86.76%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLA 60
           MD  FLTYN VVLRRSD+ SLE  NYLND IIDFYF YL   YQS +ILLVPTSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSL 120
           N  DWD FIAT+ESLNFH KKLI FTVNDNSNFSSS GGSHWSLLVYCRKRNLFMHYDSL
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSSS-GGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 HMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQ 180
           HMMN E AF+IYESVNSY+SYDSAEF+ECRAPRQRNGYDCGLYVMEIARVI E YFN DQ
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQ 180

Query: 181 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQ 220
           F++EDEICGSFNV++ VNC +VESNMRSE+LQLI  L Q
Sbjct: 181 FQNEDEICGSFNVVQRVNCKHVESNMRSEMLQLILGLNQ 218

[Close]

BLAST of Spg008645 vs. ExPASy TrEMBL
Match: A0A6J1GPM3 (NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456312 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.2e-91
Identity = 171/221 (77.38%), Postives = 188/221 (85.07%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLA 60
           M   FLTY DVV++  DLHSLEGRNYLNDTIIDFYF YLS  YQS+EIL +PTS+SFLL 
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSL 120
           NTLDWD+FI+TIESLN H KKLI F VNDNSN SSS GGSHWSLLVYCRKRNLFMHYDSL
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSSS-GGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 HMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQ 180
             MN EPAF++YESV+ Y++YD AEF+ECRAPRQ NGYDCGLYVME ARVI E YFNVDQ
Sbjct: 121 STMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQ 180

Query: 181 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQEE 222
           FRS+DEI G  NVMRSV   YVESNMRSE++QLI RLKQE+
Sbjct: 181 FRSKDEISGPINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 220

[Close]

BLAST of Spg008645 vs. ExPASy TrEMBL
Match: A0A6J1FTN2 (NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447200 PE=3 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 2.4e-90
Identity = 169/213 (79.34%), Postives = 184/213 (86.38%), Query Frame = 0


Query: 1   MDTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLA 60
           MD  FLTYN VVLRRSD+ SLE  NYLND IIDFYF YL   YQS +ILL+ TSISFLL 
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLG 60

Query: 61  NTLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSL 120
           N  DWD F+AT+ESLNFH KKLI FTVNDNSNFSSS GGSHWSLLVYCRKRNLFMHYDSL
Sbjct: 61  NASDWDTFVATVESLNFHEKKLIFFTVNDNSNFSSS-GGSHWSLLVYCRKRNLFMHYDSL 120

Query: 121 HMMNYEPAFSIYESVNSYVSYDSAEFIECRAPRQRNGYDCGLYVMEIARVICECYFNVDQ 180
           HMMN E AF+IYESVNSY+SYDSAEF+ECRAPRQRN YDCGLYVMEIARVI E YFN DQ
Sbjct: 121 HMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQ 180

Query: 181 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQL 214
           F++EDEICGSFNV++SVNC +VESNMRSE + +
Sbjct: 181 FQNEDEICGSFNVVQSVNCKHVESNMRSESINI 212

[Close]

BLAST of Spg008645 vs. ExPASy TrEMBL
Match: A0A7J6VHN4 (Sentrin-specific protease OS=Thalictrum thalictroides OX=46969 GN=FRX31_025797 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 2.4e-42
Identity = 107/217 (49.31%), Postives = 141/217 (64.98%), Query Frame = 0


Query: 6   LTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLANTLDW 65
           L+Y+DVVLRRSDL  L GR++LND II+FYF+YL++ Y S +ILLVP SISF + N  D 
Sbjct: 7   LSYSDVVLRRSDLDILSGRHFLNDRIIEFYFSYLASCYPSEDILLVPPSISFWIGNCPDL 66

Query: 66  DVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSLHMMNY 125
           +     +E L F  KK++IFTVNDN + S + GG HWSLL Y RK+N+F+H+DSL   N 
Sbjct: 67  ESLKDFVEPLKFPDKKMVIFTVNDNEDVSMAEGGCHWSLLAYERKKNVFVHHDSLAGSNR 126

Query: 126 EPAFSIYESVNSYVSYDSAE----FIEC-RAPRQRNGYDCGLYVMEIARVICECYFNVDQ 185
             A  +Y+ V  ++   + E    FI+C  +P+Q NGYDCGLYV  IA+VICE Y     
Sbjct: 127 WHAKRLYKVVVRFMGISATEVDAPFIDCTTSPQQINGYDCGLYVTAIAKVICEWYVT--- 186

Query: 186 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRL 218
            RSED      ++   +  + V SNMRSE+L LI  L
Sbjct: 187 GRSEDMDLWFTSLKTKITTSTV-SNMRSEILLLIKDL 219

[Close]

BLAST of Spg008645 vs. ExPASy TrEMBL
Match: A0A2G5D630 (ULP_PROTEASE domain-containing protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_02700117v1 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 4.1e-42
Identity = 107/217 (49.31%), Postives = 142/217 (65.44%), Query Frame = 0


Query: 6   LTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLANTLDW 65
           L+YNDVVLRRSDL  L G ++LND II+FYF+YL++ Y S +ILLVP SISF ++N  D 
Sbjct: 11  LSYNDVVLRRSDLDILSGPHFLNDRIIEFYFSYLASCYASEDILLVPPSISFWISNCPDI 70

Query: 66  DVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSLHMMNY 125
           +     +E L F  KK++IF VN+N + S + GGSHWSLL Y RK+N+F+H+DSL   N 
Sbjct: 71  ESLKDFVEPLKFPDKKMVIFPVNNNDDVSMAEGGSHWSLLAYERKKNVFVHHDSLASSNR 130

Query: 126 EPAFSIYESVNSYV----SYDSAEFIEC-RAPRQRNGYDCGLYVMEIARVICECYFNVDQ 185
             A  +Y+ V  ++    S   A FI+C  +P+Q NGYDCGLYV  IA+VICE Y N   
Sbjct: 131 CHAKRLYKGVVKFMGISDSKVDAPFIDCTTSPQQMNGYDCGLYVTAIAKVICEWYVN--- 190

Query: 186 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRL 218
            RSED      ++   +  + V SNMR+E+L LI  L
Sbjct: 191 GRSEDMDSWFTSLKTQITTSSV-SNMRNEILLLIKGL 223

[Close]

BLAST of Spg008645 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 8.5e-40
Identity = 92/223 (41.26%), Postives = 140/223 (62.78%), Query Frame = 0


Query: 2   DTTFLTYNDVVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLAN 61
           D   L+Y DVVLRRSDL  L G  +LND +I+FY ++LST + S  I L+P SI+F ++N
Sbjct: 7   DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66

Query: 62  TLDWDVFIATIESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYCRKRNLFMHYDSLH 121
             D +     ++ LN   K L+I  VNDNSN   + GG HWSLLVY ++ N F+H+DS  
Sbjct: 67  CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126

Query: 122 MMNYEPAFSIYESVNSYVSYDSAEFIECR-APRQRNGYDCGLYVMEIARVICECYFNVDQ 181
            +N   A  ++++V+ +VS   A + EC   P+Q+NGYDCG++++  ARVICE +F+   
Sbjct: 127 GVNRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGG 186

Query: 182 FRSEDEICGSFNVMRSVNCNYVESNMRSELLQLISRLKQEETN 224
            ++ DE+  + NV  +V    + +++R E+L LI +L  E  +
Sbjct: 187 MKNRDELWFA-NVKETV--PDLVNHLREEILALIKKLMSESVS 225

[Close]

BLAST of Spg008645 vs. TAIR 10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-05
Identity = 50/216 (23.15%), Postives = 79/216 (36.57%), Query Frame = 0


Query: 11  VVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLANTLDWDVFIA 70
           V + + D+  L+   ++NDTIIDFY  YL    Q+ E        SF      D D   +
Sbjct: 426 VSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPS 485

Query: 71  TI--------------ESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYC-------- 130
           +I                ++  GK  I   VN N          HWSL+V C        
Sbjct: 486 SIADGKAAFLRVRKWTRKVDMFGKDYIFVPVNYN---------LHWSLIVICHPGEVANR 545

Query: 131 --------RKRNLFMHYDSLH-----MMNYEPAFSIYE--------SVNSYVSYDSAEFI 183
                   +K    +H DS+      + N    +   E        S +    + +  F+
Sbjct: 546 TDLDLDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFV 605

[Close]

BLAST of Spg008645 vs. TAIR 10
Match: AT1G09730.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-05
Identity = 50/216 (23.15%), Postives = 79/216 (36.57%), Query Frame = 0


Query: 11  VVLRRSDLHSLEGRNYLNDTIIDFYFTYLSTWYQSHEILLVPTSISFLLANTLDWDVFIA 70
           V + + D+  L+   ++NDTIIDFY  YL    Q+ E        SF      D D   +
Sbjct: 394 VSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPS 453

Query: 71  TI--------------ESLNFHGKKLIIFTVNDNSNFSSSGGGSHWSLLVYC-------- 130
           +I                ++  GK  I   VN N          HWSL+V C        
Sbjct: 454 SIADGKAAFLRVRKWTRKVDMFGKDYIFVPVNYN---------LHWSLIVICHPGEVANR 513

Query: 131 --------RKRNLFMHYDSLH-----MMNYEPAFSIYE--------SVNSYVSYDSAEFI 183
                   +K    +H DS+      + N    +   E        S +    + +  F+
Sbjct: 514 TDLDLDDSKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFV 573

The following BLAST results are available for this feature:

BLAST of Spg008645 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988745.1	7.4e-94	81.28	NEDD8-specific protease 1-like [Cucurbita maxima]	[more]
KAG7014407.1	1.8e-92	77.13	NEDD8-specific protease 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022953908.1	4.5e-91	77.38	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
XP_022942008.1	4.9e-90	79.34	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
XP_023549404.1	3.5e-88	75.11	NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg008645 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSS7	1.2e-38	41.26	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
Q5FVJ8	7.1e-23	31.74	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]
Q96LD8	1.2e-22	34.07	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
Q9D2Z4	4.6e-22	34.07	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
Q54XR2	2.8e-19	26.45	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]

BLAST of Spg008645 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JKF6	3.6e-94	81.28	NEDD8-specific protease 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485989 PE=3 ...	[more]
A0A6J1GPM3	2.2e-91	77.38	NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456312 PE=...	[more]
A0A6J1FTN2	2.4e-90	79.34	NEDD8-specific protease 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447200 PE=...	[more]
A0A7J6VHN4	2.4e-42	49.31	Sentrin-specific protease OS=Thalictrum thalictroides OX=46969 GN=FRX31_025797 P...	[more]
A0A2G5D630	4.1e-42	49.31	ULP_PROTEASE domain-containing protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_...	[more]

BLAST of Spg008645 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60190.1	8.5e-40	41.26	Cysteine proteinases superfamily protein	[more]
AT1G09730.1	1.1e-05	23.15	Cysteine proteinases superfamily protein	[more]
AT1G09730.2	1.1e-05	23.15	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 26..172 e-value: 4.3E-11 score: 43.1
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 11..171 score: 15.734093
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 1..220 e-value: 1.8E-56 score: 193.2
None	No IPR available	PANTHER	PTHR46468:SF2	PROTEASE, SENP8, PUTATIVE-RELATED	coord: 145..219 coord: 4..143
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 145..219 coord: 4..143
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 2..218

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg008645.1	Spg008645.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0019784	NEDD8-specific protease activity
molecular_function	GO:0008234	cysteine-type peptidase activity